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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

Pret-056

Organism
African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathway:iDNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 2By similarity
Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
Active sitei108 – 1081PROSITE-ProRule annotation
Metal bindingi164 – 1641Iron 2By similarity
Metal bindingi198 – 1981Iron 2By similarity
Metal bindingi201 – 2011Iron 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Ordered Locus Names:Pret-056
OrganismiAfrican swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Taxonomic identifieri561443 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000859 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Ribonucleoside-diphosphate reductase small chainPRO_0000355533Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C8I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C8I1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELLIENSH RFTIFPIQHP ECWNWYKKLE SLTWTAQEVD MCKDIDDWEA
60 70 80 90 100
MPKSQREFYK QILAFFVVAD EIVIENLLTN FMREIKVKEV LYFYTMQAAQ
110 120 130 140 150
ECVHSEAYSI QVKTLIPDEK EQQRIFSGIE KHPIIKKMAQ WVRQWMDPTK
160 170 180 190 200
NSLGERLVGF AAVEGILFQN HFVAIQFLKE QNIMPGLVSY NEFISRDEGV
210 220 230 240 250
HCSFACFLIS NYVYNIPEER IIHKILKEAV ELVDEFINYA FDKARGRVPG
260 270 280 290 300
FSKEMLFQYI RYFTDNLCFM MQCKSIYKVG NPFPQMTKFF LNEVEKTNFF
310 320
ELRPTQYQNC VKDDAFAFKL FLNDDDF
Length:327
Mass (Da):38,958
Last modified:November 25, 2008 - v1
Checksum:i7739D9CC17B31494
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261363 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261363 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortaliP0C8I1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "African swine fever virus genomes."
    Kutish G.F., Rock D.L.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR2_ASFP4
AccessioniPrimary (citable) accession number: P0C8I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: April 1, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.