Reviewed, UniProtKB/Swiss-Prot P0C8I0 (RIR2_ASFK5)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase small chain
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase small subunit

Gene names

Ordered Locus Names:

Ken-056

Organism

African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)

Taxonomic identifier

561445 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small chain By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Ribonucleoside-diphosphate reductase small chain		PRO_0000355532
Sites
	Active site	108	1	By similarity
	Metal binding	70	1	Iron 1 By similarity
	Metal binding	101	1	Iron 1 By similarity
	Metal binding	101	1	Iron 2 By similarity
	Metal binding	104	1	Iron 1 By similarity
	Metal binding	164	1	Iron 2 By similarity
	Metal binding	198	1	Iron 2 By similarity
	Metal binding	201	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8I0-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 63C2B342A1A6C94C	FASTA	327	38,885
	10 20 30 40 50 60 MEELLIEDSQ RFTIFPIQHP ECWNWYKKLE SLTWTAQEVD MCKDIDDWEA MPKPQREFYK 70 80 90 100 110 120 QILAFFVVAD EIVIENLLTN FMREIKVKEV LYFYTMQAAQ ECVHSEAYSI QVKTLIPDEK 130 140 150 160 170 180 EQQRIFSGIE KHPIIRKMAQ WVQQWMDPVK NSLGERLVGF AAVEGILFQN HFVAIQFLKE 190 200 210 220 230 240 QNIMPGLVSY NEFISRDEGM HCSFACFLIS NYLNNIPEEK IIHKILKEAV ELVDEFISYA 250 260 270 280 290 300 FDKARGSVPG FSKEMLFQYI RYFTDNLCFM LQCKSIYKVE NPFPQMTKFF LNEVEKTNFF 310 320 ELRPTQYQNC VKDDAFAFKL FLNDDDF

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261360 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000358. Ribonucl_redctse. [Graphical view]
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_ASFK5

Accession

Primary (citable) accession number: P0C8I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	June 16, 2009
This is version 3 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)