Reviewed, UniProtKB/Swiss-Prot P0C8H9 (RIR1_ASFWA)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase large subunit
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase large subunit

Gene names

Ordered Locus Names:

War-055

Organism

African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV)

Taxonomic identifier

561444 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small chain By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Developmental stage	Early protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 778	778	Ribonucleoside-diphosphate reductase large subunit		PRO_0000355221
Sites
	Active site	193	1	Hydrogen atom transfer By similarity
	Active site	419	1	Proton acceptor By similarity
	Active site	421	1	Proton acceptor By similarity
	Active site	423	1	Proton acceptor By similarity
	Active site	439	1	Hydrogen atom transfer By similarity
	Active site	747	1	Electron transfer By similarity
	Active site	748	1	Electron transfer By similarity
	Site	200	1	Allosteric effector binding By similarity
	Site	230	1	Allosteric effector binding By similarity
	Site	773	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	776	1	Interacts with thioredoxin/glutaredoxin By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8H9-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 5F0D50259308371F	FASTA	778	87,402
	10 20 30 40 50 60 METFCIETLA SDTYGKALNV DLDRLSQAQI KYTLQELISY CSALTILHYD YSTLAARLSV 70 80 90 100 110 120 YQLHQSTASS FSKAVRLQAA QSCSRLSPQF VDVVYKYKAI FDSYIDYNRD YKLSLLGIET 130 140 150 160 170 180 MKNSYLLKNK DGVIMERPQD AYMRVAIMIY GMGKVVNIKM ILLTYDLLSQ HIITHASPTM 190 200 210 220 230 240 FNAGTKKPQL SSCFLLNVND NLENLYDMVK TAGIISGGGG GIGLCLSGIR AKNSFISGSG 250 260 270 280 290 300 LRSNGIQNYI MLQNASQCYA NQGGLRPGAY AVYLELWHQD IFTFLEMPRL KGQMAEQRLN 310 320 330 340 350 360 APNLKYGLWV PDLFMEILED QIHNRGDGRW YLFSPDQAPN LHKVFDLERS QHENAHREFK 370 380 390 400 410 420 KLYYQYVAEK RYTGVTTAKE IIKAWFKTVI QVGNPYIGFK DAINRKSNLS HVGTITNSNL 430 440 450 460 470 480 CIEVTIPCWE GDKAEQGVCN LAAVNLAAFI RENGYDYRGL IEASGNVTEN LDNIIDNGYY 490 500 510 520 530 540 PTEATRRSNM RHRPIGIGVF GLADVFASLK IKFGSPEAIA MDEAIHAALY YGAMRRSIEL 550 560 570 580 590 600 AKEKGSHPSF PGSAASKGLL QPDLWVRCGD LIPSWEERVA QTTQGVLTRK SWRQLRLAAI 610 620 630 640 650 660 QGVRNGYLTA LMPTATSSNS TGKNECFEPF TSNLYTRRTL SGEFIVLNKY LIDDLKEINL 670 680 690 700 710 720 WTEAIQLQLL NAGGSIQHIL DIPAEIRDRY KTSREMNQKI LTKHAAARNP FVSQSMSLNY 730 740 750 760 770 YFYEPELSQV LTVLVLGWKK GLTTGSYYCH FSPGAGTQKK IIRNSEKACN ADCEACLL

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY261366 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_ASFWA

Accession

Primary (citable) accession number: P0C8H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	July 28, 2009
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)