Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0C8H8

- RIR1_ASFP4

UniProt

P0C8H8 - RIR1_ASFP4

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

Pret-057

Organism
African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei177 – 1771SubstrateBy similarity
Sitei193 – 1931Important for hydrogen atom transferBy similarity
Sitei200 – 2001Allosteric effector bindingBy similarity
Binding sitei221 – 2211Substrate; via amide nitrogenBy similarity
Sitei230 – 2301Allosteric effector bindingBy similarity
Active sitei419 – 4191Proton acceptorBy similarity
Active sitei421 – 4211Cysteine radical intermediateBy similarity
Active sitei423 – 4231Proton acceptorBy similarity
Sitei439 – 4391Important for hydrogen atom transferBy similarity
Sitei747 – 7471Important for electron transferBy similarity
Sitei748 – 7481Important for electron transferBy similarity
Sitei773 – 7731Interacts with thioredoxin/glutaredoxinBy similarity
Sitei776 – 7761Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Ordered Locus Names:Pret-057
OrganismiAfrican swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Taxonomic identifieri561443 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000859: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 778778Ribonucleoside-diphosphate reductase large subunitPRO_0000355220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi193 ↔ 439Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1932Substrate bindingBy similarity
Regioni419 – 4235Substrate bindingBy similarity
Regioni613 – 6175Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C8H8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METFFIETLA SDVYGKALNV DLDRLSQAQI KYTLQELISY CSALTILHYD
60 70 80 90 100
YSTLAARLSV YQLHQSTASS FSKAVRLQAA QSCSRLSPQF VDVVYKYKAI
110 120 130 140 150
FDSYIDYSRD YKLSLLGIET MKNSYLLKNK DGVIMERPQD AYMRVAIMIY
160 170 180 190 200
GMGKVVNIKM ILLTYDLLSR HIITHASPTM FNAGTKKPQL SSCFLLNVND
210 220 230 240 250
NLENLYDMVK TAGIISGGGG GIGLCLSGIR AKNSFISGSG LRSNGIQNYI
260 270 280 290 300
MLQNASQCYA NQGGLRPGAY AVYLELWHQD IFTFLEMPRL KGQMAEQRLN
310 320 330 340 350
APNLKYGLWV PDLFMEILED QIHNRGDGRW YLFSPDQAPN LHKVFDLERS
360 370 380 390 400
QHENAHREFK KLYYQYVAEK RYTGVTTAKE IIKAWFKTVI QVGNPYIGFK
410 420 430 440 450
DAINRKSNLS HVGTITNSNL CIEVTIPCWE GDKAEQGVCN LAAVNLAAFI
460 470 480 490 500
RENGYDYRGL IEASGNVTEN LDNIIDNGYY PTEATRRSNM RHRPIGIGVF
510 520 530 540 550
GLADVFASLK IKFGSPEAIA MDEAIHAALY YGAMRRSIEL AKEKGSHPSF
560 570 580 590 600
PGSAASKGLL QPDLWVRCGD LIPSWEERVA QTTQGVLTRK SWRQLRLAAI
610 620 630 640 650
QGVRNGYLTA LMPTATSSNS TGKNECFEPF TSNLYTRRTL SGEFIVLNKY
660 670 680 690 700
LIDDLKEINL WTEAIQLQLL NAGGSIQHIL DIPAEIRDRY KTSREMNQKI
710 720 730 740 750
LTKHAAARNP FVSQSMSLNY YFYEPELSQV LTVLVLGWKK GLTTGSYYCH
760 770
FSPGAGTQKK IIRNSEKACN ADCEACLL
Length:778
Mass (Da):87,445
Last modified:November 25, 2008 - v1
Checksum:i9C57F84C0F3E8AE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261363 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261363 Genomic DNA. No translation available.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "African swine fever virus genomes."
    Kutish G.F., Rock D.L.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_ASFP4
AccessioniPrimary (citable) accession number: P0C8H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: October 29, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3