Reviewed, UniProtKB/Swiss-Prot P0C8H7 (RIR1_ASFK5)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase large subunit
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase large subunit

Gene names

Ordered Locus Names:

Ken-057

Organism

African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)

Taxonomic identifier

561445 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small chain By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Developmental stage	Early protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 779	779	Ribonucleoside-diphosphate reductase large subunit		PRO_0000355219
Sites
	Active site	194	1	Hydrogen atom transfer By similarity
	Active site	420	1	Proton acceptor By similarity
	Active site	422	1	Proton acceptor By similarity
	Active site	424	1	Proton acceptor By similarity
	Active site	440	1	Hydrogen atom transfer By similarity
	Active site	748	1	Electron transfer By similarity
	Active site	749	1	Electron transfer By similarity
	Site	201	1	Allosteric effector binding By similarity
	Site	231	1	Allosteric effector binding By similarity
	Site	774	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	777	1	Interacts with thioredoxin/glutaredoxin By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8H7-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 7FF7134ECDC5C861	FASTA	779	87,767
	10 20 30 40 50 60 MENFFIVKKL ASDTYGKALN VDLDRLLQAQ NKYTLQELIS YCSALTILHY DYSTLAARLS 70 80 90 100 110 120 VYLLHQSTAS SFSEAVSLQA AQSCSRLSPQ FVDVVYKYKA IFDSYIDYSR DYKLTLLGIE 130 140 150 160 170 180 TMKNSYLLKN KDGVIMERPQ DAYMRVAIMI YGMGRVVNMK MILLTYDLLS RHVITHASPT 190 200 210 220 230 240 MFNAGTKKPQ LSSCFLLNVN DNLENLYDMV KTAGIISGGG GGIGLCLSGI RAKNSFISGS 250 260 270 280 290 300 GLRSNGIQNY IVLQNASQCY ANQGGLRPGA YAVYLELWHQ DIFTFLQMPR LKGQMAEQRL 310 320 330 340 350 360 NAPNLKYGLW VPDLFMEILE DQIHDRGDGT WYLFSPDQAP NLHKVFDLER SRHKNAHREF 370 380 390 400 410 420 RKLYYQYVAE KRYTGVTTAK EIIKEWFKTV IQVGNPYIGF KDAINRKSNL SHVGTITNSN 430 440 450 460 470 480 LCIEITIPCW EGSEAEQGVC NLAAVNLAAF IRENSYDYRG LIEAAGNVTE NLDNIIDNGY 490 500 510 520 530 540 YPTEATRRSN MRHRPIGIGV FGLADVFASF KMKFGSPEAI AMDEAIHAAL YYGAMRRSVE 550 560 570 580 590 600 LAKEKGSHPS FPGSAASKGL LQPDLWVRCD DLVFSWEERV AQTTQGVLTP KKWWQLRLAA 610 620 630 640 650 660 MQGVRNGYLT ALMPTATSSN STGKNECFEP FTSNLYTRRT LSGEFIVLNK YLIDDLKEIN 670 680 690 700 710 720 LWTEAIQQQL LNAGGSIQHI LDIPAEIRER YKTSREMNQK ILTKHAAARN PFVSQSMSLN 730 740 750 760 770 YYFYEPELSQ VLTVLVLGWK KGLTTGSYYC HFSPGAGTQK KIIRNSEKAC SADCEACLL

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY261360 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_ASFK5

Accession

Primary (citable) accession number: P0C8H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	July 28, 2009
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)