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P0C8H7

- RIR1_ASFK5

UniProt

P0C8H7 - RIR1_ASFK5

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
Ken-057
Organism
African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei178 – 1781Substrate By similarity
Sitei194 – 1941Important for hydrogen atom transfer By similarity
Sitei201 – 2011Allosteric effector binding By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Sitei231 – 2311Allosteric effector binding By similarity
Active sitei420 – 4201Proton acceptor By similarity
Active sitei422 – 4221Cysteine radical intermediate By similarity
Active sitei424 – 4241Proton acceptor By similarity
Sitei440 – 4401Important for hydrogen atom transfer By similarity
Sitei748 – 7481Important for electron transfer By similarity
Sitei749 – 7491Important for electron transfer By similarity
Sitei774 – 7741Interacts with thioredoxin/glutaredoxin By similarity
Sitei777 – 7771Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Ordered Locus Names:Ken-057
OrganismiAfrican swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)
Taxonomic identifieri561445 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000861: Genome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Ribonucleoside-diphosphate reductase large subunitPRO_0000355219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi194 ↔ 440Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP0C8H7.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 1942Substrate binding By similarity
Regioni420 – 4245Substrate binding By similarity
Regioni614 – 6185Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C8H7-1 [UniParc]FASTAAdd to Basket

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MENFFIVKKL ASDTYGKALN VDLDRLLQAQ NKYTLQELIS YCSALTILHY    50
DYSTLAARLS VYLLHQSTAS SFSEAVSLQA AQSCSRLSPQ FVDVVYKYKA 100
IFDSYIDYSR DYKLTLLGIE TMKNSYLLKN KDGVIMERPQ DAYMRVAIMI 150
YGMGRVVNMK MILLTYDLLS RHVITHASPT MFNAGTKKPQ LSSCFLLNVN 200
DNLENLYDMV KTAGIISGGG GGIGLCLSGI RAKNSFISGS GLRSNGIQNY 250
IVLQNASQCY ANQGGLRPGA YAVYLELWHQ DIFTFLQMPR LKGQMAEQRL 300
NAPNLKYGLW VPDLFMEILE DQIHDRGDGT WYLFSPDQAP NLHKVFDLER 350
SRHKNAHREF RKLYYQYVAE KRYTGVTTAK EIIKEWFKTV IQVGNPYIGF 400
KDAINRKSNL SHVGTITNSN LCIEITIPCW EGSEAEQGVC NLAAVNLAAF 450
IRENSYDYRG LIEAAGNVTE NLDNIIDNGY YPTEATRRSN MRHRPIGIGV 500
FGLADVFASF KMKFGSPEAI AMDEAIHAAL YYGAMRRSVE LAKEKGSHPS 550
FPGSAASKGL LQPDLWVRCD DLVFSWEERV AQTTQGVLTP KKWWQLRLAA 600
MQGVRNGYLT ALMPTATSSN STGKNECFEP FTSNLYTRRT LSGEFIVLNK 650
YLIDDLKEIN LWTEAIQQQL LNAGGSIQHI LDIPAEIRER YKTSREMNQK 700
ILTKHAAARN PFVSQSMSLN YYFYEPELSQ VLTVLVLGWK KGLTTGSYYC 750
HFSPGAGTQK KIIRNSEKAC SADCEACLL 779
Length:779
Mass (Da):87,767
Last modified:November 25, 2008 - v1
Checksum:i7FF7134ECDC5C861
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY261360 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY261360 Genomic DNA. No translation available.

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0C8H7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "African swine fever virus genomes."
    Kutish G.F., Rock D.L.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_ASFK5
AccessioniPrimary (citable) accession number: P0C8H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: October 16, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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