Reviewed, UniProtKB/Swiss-Prot P0C8G8 (FLSO_ASFK5)

Names and origin

Protein names

Recommended name:
    FAD-linked sulfhydryl oxidase
    EC=1.8.3.2
Alternative name(s):
    p14

Gene names

Ordered Locus Names:

Ken-085

Organism

African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)

Taxonomic identifier

561445 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Involved in virion maturation By similarity.
Catalytic activity	4 R'C(R)SH + O2 = 2 R'C(R)S-S(R)CR' + 2 H2O.
Cofactor	FAD By similarity.
Subunit structure	Interacts with A151R By similarity.
Subcellular location	Host cytoplasm By similarity.
Induction	Expressed late in the infection cycle.
Sequence similarities	Belongs to the asfivirus B119L family. Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Host cytoplasm
Developmental stage	Late protein
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	FAD-linked sulfhydryl oxidase		PRO_0000355535
Regions
	Domain	1 – 97	97	ERV/ALR sulfhydryl oxidase
Amino acid modifications
	Disulfide bond	44 ↔ 47		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P0C8G8-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 7EFEBDF44C97DF40	FASTA	119	14,390
	10 20 30 40 50 60 MLHWGPKFWR ALHLYAIFFS DAPNWKEKYE AIQWILNFIE SLPCTMCRHH AFSYLTKNPL 70 80 90 100 110 TLNNSEDFQY WTFAFHNNVN KRLNKKIISW SEYKNIYEQS ILKTIEYGKT DFIGAWSSL

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY261360 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit.
Pfam	PF04777. Evr1_Alr. 1 hit. [Graphical view]
PROSITE	PS51324. ERV_ALR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLSO_ASFK5

Accession

Primary (citable) accession number: P0C8G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)