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P0C8G7

- PV22_POMCA

UniProt

P0C8G7 - PV22_POMCA

Protein

Perivitellin-2 31 kDa subunit

Gene
N/A
Organism
Pomacea canaliculata (Golden apple snail)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 13 (01 Oct 2014)
      Sequence version 2 (24 Jul 2013)
      Previous versions | rss
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    Functioni

    Is a source of both structural and energetic molecules during embryonic development.
    Neurotoxin present in eggs that is lethal when ingested. Shows wide pH range stability and is resistant to proteases trypsin and pepsin (gastric and intestinal digestion). Specifically binds mature enterocytes (binding due to the tachylectin subunit). May traverse the intestinal barrier, since it is able to orally stimulate the immune system in rats and mice. Also shows hemagglutinating activity against rabbit erythrocytes.

    GO - Molecular functioni

    1. nutrient reservoir activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Neurotoxin, Storage protein, Toxin

    Keywords - Ligandi

    Lectin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Perivitellin-2 31 kDa subunit
    Short name:
    PcPV2 31 kDa subunit
    Short name:
    PcPV2-31
    Alternative name(s):
    PV2 tachylectin subunit
    OrganismiPomacea canaliculata (Golden apple snail)
    Taxonomic identifieri400727 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaArchitaenioglossaAmpullarioideaAmpullariidaePomacea

    Pathology & Biotechi

    Toxic dosei

    LD50 is 250 µg/kg by intraperitoneal injection into mice.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 30301 PublicationAdd
    BLAST
    Chaini31 – 286256Perivitellin-2 31 kDa subunitPRO_0000355072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated. Contains four O-linked and one N-linked oligosaccharide bonds. The protein contains 2.5% of carbohydrates (high levels of mannose, galactose, and NAcGlucosamine, and small amounts of NacGalactosamine).1 Publication
    Very high density lipoprotein (VHDL). Contains 3.75% of lipids. The major lipid classes are free sterols and phospholipids and also have significant quantities of energy-providing triacylglycerides and free fatty acids.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein

    Expressioni

    Tissue specificityi

    Produced by albumen secretory cells. Found in developing eggs.1 Publication

    Developmental stagei

    Albumen secretory cells produce perivitellin-2 during the reproductive period.1 Publication

    Interactioni

    Subunit structurei

    Perivitellin-2 is a heterooctamer of 4 identical 98 kDa heterodimers, each composed of one 31 kDa and one 67 kDa subunits. The 98 kDa heterodimer subunits are held together by disulfide bridges while the heterodimers are assembled into the native perivitellin-2 octamer by non-covalent forces.2 Publications

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR006624. Beta-propeller_rpt_TECPR.
    [Graphical view]
    SMARTiSM00706. TECPR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C8G7-1 [UniParc]FASTAAdd to Basket

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    MVKKIHFIME RHASIVAFLL AVLALTESQA FTSVKLPRDE HWPYNYVSIG    50
    PAGVWAVNRQ NKLFYRTGTY GDNANMGSGW QFKQDGVGQV DVGKDKVGYI 100
    NLSGGSLFRI DGISQGNPVG GSPKSWEWWT KYIGMSLRED TRFSSRIENQ 150
    NKVLTFTFRT CFWASRITNW CFADSSYTET VTAGGSGTWI TKSQLKYKSG 200
    TFGNPDTEGG DWITVDSGSF QHVSSGSGVV LAVRSNGELV KRTGITCSLP 250
    QGSGWTSMLN GMSRVDTYGT VAWAVDTYGD LYFINL 286
    Length:286
    Mass (Da):31,584
    Last modified:July 24, 2013 - v2
    Checksum:i3DB891F4E71253CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JX155862 mRNA. Translation: AGG40752.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A snail's sting - Issue 100 of December 2008

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JX155862 mRNA. Translation: AGG40752.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR006624. Beta-propeller_rpt_TECPR.
    [Graphical view ]
    SMARTi SM00706. TECPR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel animal defenses against predation: a snail egg neurotoxin combining lectin and pore-forming chains that resembles plant defense and bacteria attack toxins."
      Dreon M.S., Frassa M.V., Ceolin M., Ituarte S., Qiu J.W., Sun J., Fernandez P.E., Heras H.
      PLoS ONE 8:E63782-E63782(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Albumen gland.
    2. "Synthesis, distribution, and levels of an egg lipoprotein from the apple snail Pomacea canaliculata (Mollusca: Gastropoda)."
      Dreon M., Lavarias S., Garin C.F., Heras H., Pollero R.J.
      J. Exp. Zool. 292:323-330(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 31-45.
      Tissue: Albumen gland.
    3. "Lipoproteins of the egg perivitelline fluid of Pomacea canaliculata snails (Mollusca: Gastropoda)."
      Garin C.F., Heras H., Pollero R.J.
      J. Exp. Zool. 276:307-314(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, LIPID COMPOSITION.
    4. "Biochemical composition and energy sources during embryo development and in early juveniles of the snail Pomacea canaliculata (Mollusca: Gastropoda)."
      Heras H., Garin C.F., Pollero R.J.
      J. Exp. Zool. 280:375-383(1998)
      Cited for: FUNCTION.
    5. "Characterization of the major egg glycolipoproteins from the perivitellin fluid of the apple snail Pomacea canaliculata."
      Dreon M.S., Heras H., Pollero R.J.
      Mol. Reprod. Dev. 68:359-364(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CARBOHYDRATE COMPOSITION, GLYCOSYLATION PATTERN.
    6. "Pallial oviduct of Pomacea canaliculata (Gastropoda): ultrastructural studies of the parenchymal cellular types involved in the metabolism of perivitellins."
      Catalan M., Dreon M.S., Heras H., Pollero R.J., Fernandez S.N., Winik B.
      Cell Tissue Res. 324:523-533(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    7. Cited for: FUNCTION, LETHAL DOSE.
    8. "Structure and stability of the neurotoxin PV2 from the eggs of the apple snail Pomacea canaliculata."
      Frassa M.V., Ceolin M., Dreon M.S., Heras H.
      Biochim. Biophys. Acta 1804:1492-1499(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiPV22_POMCA
    AccessioniPrimary (citable) accession number: P0C8G7
    Secondary accession number(s): M1TI98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: July 24, 2013
    Last modified: October 1, 2014
    This is version 13 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Mice receiving sublethal doses of toxin become immunized towards the toxin and doses up to LD(100) do not kill them.
    Does not show hemolytic activity nor caco-2 (human colorectal adenocarcinoma cells) or rat intestinal cell disruption activity.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

    External Data

    Dasty 3