Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Perivitellin-2 31 kDa subunit

Gene
N/A
Organism
Pomacea canaliculata (Golden apple snail)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is a source of both structural and energetic molecules during embryonic development.
Neurotoxin present in eggs that is lethal when ingested. Shows wide pH range stability and is resistant to proteases trypsin and pepsin (gastric and intestinal digestion). Specifically binds mature enterocytes (binding due to the tachylectin subunit). May traverse the intestinal barrier, since it is able to orally stimulate the immune system in rats and mice. Also shows hemagglutinating activity against rabbit erythrocytes.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Neurotoxin, Storage protein, Toxin

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Perivitellin-2 31 kDa subunit
Short name:
PcPV2 31 kDa subunit
Short name:
PcPV2-31
Alternative name(s):
PV2 tachylectin subunit
OrganismiPomacea canaliculata (Golden apple snail)
Taxonomic identifieri400727 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaArchitaenioglossaAmpullarioideaAmpullariidaePomacea

Pathology & Biotechi

Toxic dosei

LD50 is 250 µg/kg by intraperitoneal injection into mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 301 PublicationAdd BLAST30
ChainiPRO_000035507231 – 286Perivitellin-2 31 kDa subunitAdd BLAST256

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi101N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated. Contains four O-linked and one N-linked oligosaccharide bonds. The protein contains 2.5% of carbohydrates (high levels of mannose, galactose, and NAcGlucosamine, and small amounts of NacGalactosamine).1 Publication
Very high density lipoprotein (VHDL). Contains 3.75% of lipids. The major lipid classes are free sterols and phospholipids and also have significant quantities of energy-providing triacylglycerides and free fatty acids.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Expressioni

Tissue specificityi

Produced by albumen secretory cells. Found in developing eggs.1 Publication

Developmental stagei

Albumen secretory cells produce perivitellin-2 during the reproductive period.1 Publication

Interactioni

Subunit structurei

Perivitellin-2 is a heterooctamer of 4 identical 98 kDa heterodimers, each composed of one 31 kDa and one 67 kDa subunits. The 98 kDa heterodimer subunits are held together by disulfide bridges while the heterodimers are assembled into the native perivitellin-2 octamer by non-covalent forces.2 Publications

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR006624. Beta-propeller_rpt_TECPR.
[Graphical view]
PfamiPF06462. Hyd_WA. 1 hit.
[Graphical view]
SMARTiSM00706. TECPR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C8G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKIHFIME RHASIVAFLL AVLALTESQA FTSVKLPRDE HWPYNYVSIG
60 70 80 90 100
PAGVWAVNRQ NKLFYRTGTY GDNANMGSGW QFKQDGVGQV DVGKDKVGYI
110 120 130 140 150
NLSGGSLFRI DGISQGNPVG GSPKSWEWWT KYIGMSLRED TRFSSRIENQ
160 170 180 190 200
NKVLTFTFRT CFWASRITNW CFADSSYTET VTAGGSGTWI TKSQLKYKSG
210 220 230 240 250
TFGNPDTEGG DWITVDSGSF QHVSSGSGVV LAVRSNGELV KRTGITCSLP
260 270 280
QGSGWTSMLN GMSRVDTYGT VAWAVDTYGD LYFINL
Length:286
Mass (Da):31,584
Last modified:July 24, 2013 - v2
Checksum:i3DB891F4E71253CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX155862 mRNA. Translation: AGG40752.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

A snail's sting - Issue 100 of December 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX155862 mRNA. Translation: AGG40752.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR006624. Beta-propeller_rpt_TECPR.
[Graphical view]
PfamiPF06462. Hyd_WA. 1 hit.
[Graphical view]
SMARTiSM00706. TECPR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPV22_POMCA
AccessioniPrimary (citable) accession number: P0C8G7
Secondary accession number(s): M1TI98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: July 24, 2013
Last modified: September 16, 2015
This is version 15 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Mice receiving sublethal doses of toxin become immunized towards the toxin and doses up to LD(100) do not kill them.
Does not show hemolytic activity nor caco-2 (human colorectal adenocarcinoma cells) or rat intestinal cell disruption activity.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.