Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0C8G6

- PV21_POMCA

UniProt

P0C8G6 - PV21_POMCA

Protein

Perivitellin-2 67 kDa subunit

Gene
N/A
Organism
Pomacea canaliculata (Golden apple snail)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 13 (01 Oct 2014)
      Sequence version 2 (24 Jul 2013)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Is a source of both structural and energetic molecules during embryonic development.
    Neurotoxin present in eggs that is lethal when ingested. Shows wide pH range stability and is resistant to proteases trypsin and pepsin (gastric and intestinal digestion). Specifically binds mature enterocytes (binding due to the tachylectin subunit). May traverse the intestinal barrier, since it is able to orally stimulate the immune system in rats and mice. Also shows hemagglutinating activity against rabbit erythrocytes.

    GO - Molecular functioni

    1. nutrient reservoir activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Neurotoxin, Storage protein, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Perivitellin-2 67 kDa subunit
    Short name:
    PcPV2 67 kDa subunit
    Short name:
    PcPV2-67
    Alternative name(s):
    PV2 MACPF subunit
    OrganismiPomacea canaliculata (Golden apple snail)
    Taxonomic identifieri400727 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaArchitaenioglossaAmpullarioideaAmpullariidaePomacea

    Pathology & Biotechi

    Toxic dosei

    LD50 is 250 µg/kg by intraperitoneal injection into mice.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 565539Perivitellin-2 67 kDa subunitPRO_0000355071Add
    BLAST

    Post-translational modificationi

    Glycosylated. Contains four O-linked and one N-linked oligosaccharide bonds. The protein contains 2.5% of carbohydrates (high levels of mannose, galactose, and NAcGlucosamine, and small amounts of NacGalactosamine).1 Publication
    Very high density lipoprotein (VHDL). Contains 3.75% of lipids. The major lipid classes are free sterols and phospholipids and also have significant quantities of energy-providing triacylglycerides and free fatty acids.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein

    Expressioni

    Tissue specificityi

    Produced by albumen secretory cells. Found in developing eggs.1 Publication

    Developmental stagei

    Albumen secretory cells produce perivitellin-2 during the reproductive period.1 Publication

    Interactioni

    Subunit structurei

    Perivitellin-2 is a heterooctamer of 4 identical 98 kDa heterodimers, each composed of one 31 kDa and one 67 kDa subunits. The 98 kDa heterodimer subunits are held together by disulfide bridges while the heterodimers are assembled into the native perivitellin-2 octamer by non-covalent forces.2 Publications

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 340314MACPFPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MACPF domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR020864. MACPF.
    IPR020863. MACPF_CS.
    [Graphical view]
    PfamiPF01823. MACPF. 1 hit.
    [Graphical view]
    PROSITEiPS00279. MACPF_1. 1 hit.
    PS51412. MACPF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0C8G6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLRWWVVS QLLLLIVVCI LDHSEGARVC PKIVPGLDKL RVGVDITKLD    50
    LLPLFDLGDN GFRSAVADYT CDRGQTTVVD GESFDVPDQV DSVVIESSGQ 100
    QTSSVTTIKS ESQISQALSI SAGISVDTAK AGFSSSASYA EMQEAITKYG 150
    RTVSQMSAVY TTCSANLSPN LLLGQNPLQT LSRLPSDFTA DTEGYYDFIK 200
    TYGTHYFNKG KLGGMFLFTS ETDMSYFQNK NSQQVEANIK ATFASILSTE 250
    TGGSSDQSKE VIEFKESSLI TAKFFGGRTN LAADGLTKWQ PTIAKLPYFM 300
    SGTLSTISSL IADTTKRASM ELAVKNYLLK AKVANLDRLT YIRLNSWTVG 350
    HNELRDLSAQ LQNLKKKTIF SDEDEKLLQS IEDQVSVPAW FSDRTTFCFR 400
    STAVGSADQC NGQSTSTLCA EPNRYTQQYM DKTYLGDTGC RLVWKLSTTE 450
    SSDWFKSVKV NFRWYPTWSP CACGPVGTPF TISAPANSWT QDYLDVTNPK 500
    FGECMLQWMI EVPPTATLWA KNLEFCIDFT CGKKKQCVDA NHWTEPYLDI 550
    SAHEACGMSW ALIAK 565
    Length:565
    Mass (Da):62,518
    Last modified:July 24, 2013 - v2
    Checksum:iED003AC8B36F1486
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JX155861 mRNA. Translation: AGG40751.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A snail's sting - Issue 100 of December 2008

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JX155861 mRNA. Translation: AGG40751.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR020864. MACPF.
    IPR020863. MACPF_CS.
    [Graphical view ]
    Pfami PF01823. MACPF. 1 hit.
    [Graphical view ]
    PROSITEi PS00279. MACPF_1. 1 hit.
    PS51412. MACPF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel animal defenses against predation: a snail egg neurotoxin combining lectin and pore-forming chains that resembles plant defense and bacteria attack toxins."
      Dreon M.S., Frassa M.V., Ceolin M., Ituarte S., Qiu J.W., Sun J., Fernandez P.E., Heras H.
      PLoS ONE 8:E63782-E63782(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Albumen gland.
    2. "Synthesis, distribution, and levels of an egg lipoprotein from the apple snail Pomacea canaliculata (Mollusca: Gastropoda)."
      Dreon M., Lavarias S., Garin C.F., Heras H., Pollero R.J.
      J. Exp. Zool. 292:323-330(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-44.
      Tissue: Albumen gland.
    3. "Lipoproteins of the egg perivitelline fluid of Pomacea canaliculata snails (Mollusca: Gastropoda)."
      Garin C.F., Heras H., Pollero R.J.
      J. Exp. Zool. 276:307-314(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, LIPID COMPOSITION.
    4. "Biochemical composition and energy sources during embryo development and in early juveniles of the snail Pomacea canaliculata (Mollusca: Gastropoda)."
      Heras H., Garin C.F., Pollero R.J.
      J. Exp. Zool. 280:375-383(1998)
      Cited for: FUNCTION.
    5. "Characterization of the major egg glycolipoproteins from the perivitellin fluid of the apple snail Pomacea canaliculata."
      Dreon M.S., Heras H., Pollero R.J.
      Mol. Reprod. Dev. 68:359-364(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CARBOHYDRATE COMPOSITION, GLYCOSYLATION PATTERN.
    6. "Pallial oviduct of Pomacea canaliculata (Gastropoda): ultrastructural studies of the parenchymal cellular types involved in the metabolism of perivitellins."
      Catalan M., Dreon M.S., Heras H., Pollero R.J., Fernandez S.N., Winik B.
      Cell Tissue Res. 324:523-533(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    7. Cited for: FUNCTION, LETHAL DOSE.
    8. "Structure and stability of the neurotoxin PV2 from the eggs of the apple snail Pomacea canaliculata."
      Frassa M.V., Ceolin M., Dreon M.S., Heras H.
      Biochim. Biophys. Acta 1804:1492-1499(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiPV21_POMCA
    AccessioniPrimary (citable) accession number: P0C8G6
    Secondary accession number(s): M1TA54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: July 24, 2013
    Last modified: October 1, 2014
    This is version 13 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Mice receiving sublethal doses of toxin become immunized towards the toxin and doses up to LD(100) do not kill them.
    Does not show hemolytic activity nor caco-2 (human colorectal adenocarcinoma cells) or rat intestinal cell disruption activity.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3