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P0C8G6

- PV21_POMCA

UniProt

P0C8G6 - PV21_POMCA

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Protein

Perivitellin-2 67 kDa subunit

Gene
N/A
Organism
Pomacea canaliculata (Golden apple snail)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Is a source of both structural and energetic molecules during embryonic development.
Neurotoxin present in eggs that is lethal when ingested. Shows wide pH range stability and is resistant to proteases trypsin and pepsin (gastric and intestinal digestion). Specifically binds mature enterocytes (binding due to the tachylectin subunit). May traverse the intestinal barrier, since it is able to orally stimulate the immune system in rats and mice. Also shows hemagglutinating activity against rabbit erythrocytes.

GO - Molecular functioni

  1. nutrient reservoir activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Neurotoxin, Storage protein, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Perivitellin-2 67 kDa subunit
Short name:
PcPV2 67 kDa subunit
Short name:
PcPV2-67
Alternative name(s):
PV2 MACPF subunit
OrganismiPomacea canaliculata (Golden apple snail)
Taxonomic identifieri400727 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaArchitaenioglossaAmpullarioideaAmpullariidaePomacea

Pathology & Biotechi

Toxic dosei

LD50 is 250 µg/kg by intraperitoneal injection into mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 565539Perivitellin-2 67 kDa subunitPRO_0000355071Add
BLAST

Post-translational modificationi

Glycosylated. Contains four O-linked and one N-linked oligosaccharide bonds. The protein contains 2.5% of carbohydrates (high levels of mannose, galactose, and NAcGlucosamine, and small amounts of NacGalactosamine).1 Publication
Very high density lipoprotein (VHDL). Contains 3.75% of lipids. The major lipid classes are free sterols and phospholipids and also have significant quantities of energy-providing triacylglycerides and free fatty acids.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Expressioni

Tissue specificityi

Produced by albumen secretory cells. Found in developing eggs.1 Publication

Developmental stagei

Albumen secretory cells produce perivitellin-2 during the reproductive period.1 Publication

Interactioni

Subunit structurei

Perivitellin-2 is a heterooctamer of 4 identical 98 kDa heterodimers, each composed of one 31 kDa and one 67 kDa subunits. The 98 kDa heterodimer subunits are held together by disulfide bridges while the heterodimers are assembled into the native perivitellin-2 octamer by non-covalent forces.2 Publications

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 340314MACPFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MACPF domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR020864. MACPF.
IPR020863. MACPF_CS.
[Graphical view]
PfamiPF01823. MACPF. 1 hit.
[Graphical view]
PROSITEiPS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C8G6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQLRWWVVS QLLLLIVVCI LDHSEGARVC PKIVPGLDKL RVGVDITKLD
60 70 80 90 100
LLPLFDLGDN GFRSAVADYT CDRGQTTVVD GESFDVPDQV DSVVIESSGQ
110 120 130 140 150
QTSSVTTIKS ESQISQALSI SAGISVDTAK AGFSSSASYA EMQEAITKYG
160 170 180 190 200
RTVSQMSAVY TTCSANLSPN LLLGQNPLQT LSRLPSDFTA DTEGYYDFIK
210 220 230 240 250
TYGTHYFNKG KLGGMFLFTS ETDMSYFQNK NSQQVEANIK ATFASILSTE
260 270 280 290 300
TGGSSDQSKE VIEFKESSLI TAKFFGGRTN LAADGLTKWQ PTIAKLPYFM
310 320 330 340 350
SGTLSTISSL IADTTKRASM ELAVKNYLLK AKVANLDRLT YIRLNSWTVG
360 370 380 390 400
HNELRDLSAQ LQNLKKKTIF SDEDEKLLQS IEDQVSVPAW FSDRTTFCFR
410 420 430 440 450
STAVGSADQC NGQSTSTLCA EPNRYTQQYM DKTYLGDTGC RLVWKLSTTE
460 470 480 490 500
SSDWFKSVKV NFRWYPTWSP CACGPVGTPF TISAPANSWT QDYLDVTNPK
510 520 530 540 550
FGECMLQWMI EVPPTATLWA KNLEFCIDFT CGKKKQCVDA NHWTEPYLDI
560
SAHEACGMSW ALIAK
Length:565
Mass (Da):62,518
Last modified:July 24, 2013 - v2
Checksum:iED003AC8B36F1486
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX155861 mRNA. Translation: AGG40751.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

A snail's sting - Issue 100 of December 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JX155861 mRNA. Translation: AGG40751.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR020864. MACPF.
IPR020863. MACPF_CS.
[Graphical view ]
Pfami PF01823. MACPF. 1 hit.
[Graphical view ]
PROSITEi PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel animal defenses against predation: a snail egg neurotoxin combining lectin and pore-forming chains that resembles plant defense and bacteria attack toxins."
    Dreon M.S., Frassa M.V., Ceolin M., Ituarte S., Qiu J.W., Sun J., Fernandez P.E., Heras H.
    PLoS ONE 8:E63782-E63782(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Albumen gland.
  2. "Synthesis, distribution, and levels of an egg lipoprotein from the apple snail Pomacea canaliculata (Mollusca: Gastropoda)."
    Dreon M., Lavarias S., Garin C.F., Heras H., Pollero R.J.
    J. Exp. Zool. 292:323-330(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-44.
    Tissue: Albumen gland.
  3. "Lipoproteins of the egg perivitelline fluid of Pomacea canaliculata snails (Mollusca: Gastropoda)."
    Garin C.F., Heras H., Pollero R.J.
    J. Exp. Zool. 276:307-314(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, LIPID COMPOSITION.
  4. "Biochemical composition and energy sources during embryo development and in early juveniles of the snail Pomacea canaliculata (Mollusca: Gastropoda)."
    Heras H., Garin C.F., Pollero R.J.
    J. Exp. Zool. 280:375-383(1998)
    Cited for: FUNCTION.
  5. "Characterization of the major egg glycolipoproteins from the perivitellin fluid of the apple snail Pomacea canaliculata."
    Dreon M.S., Heras H., Pollero R.J.
    Mol. Reprod. Dev. 68:359-364(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CARBOHYDRATE COMPOSITION, GLYCOSYLATION PATTERN.
  6. "Pallial oviduct of Pomacea canaliculata (Gastropoda): ultrastructural studies of the parenchymal cellular types involved in the metabolism of perivitellins."
    Catalan M., Dreon M.S., Heras H., Pollero R.J., Fernandez S.N., Winik B.
    Cell Tissue Res. 324:523-533(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. Cited for: FUNCTION, LETHAL DOSE.
  8. "Structure and stability of the neurotoxin PV2 from the eggs of the apple snail Pomacea canaliculata."
    Frassa M.V., Ceolin M., Dreon M.S., Heras H.
    Biochim. Biophys. Acta 1804:1492-1499(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiPV21_POMCA
AccessioniPrimary (citable) accession number: P0C8G6
Secondary accession number(s): M1TA54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: July 24, 2013
Last modified: October 1, 2014
This is version 13 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Mice receiving sublethal doses of toxin become immunized towards the toxin and doses up to LD(100) do not kill them.
Does not show hemolytic activity nor caco-2 (human colorectal adenocarcinoma cells) or rat intestinal cell disruption activity.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3