P0C8G4 (UBC_ASFP4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein
Ubiquitin-protein ligase

Gene names

Name:	UBC
Ordered Locus Names:	Pret-156

Organism

African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV) [Complete proteome]

Taxonomic identifier

561443 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus › African swine fever virus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Induction	Mainly late in infection.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Developmental stage	Late protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Ubiquitin-conjugating enzyme E2		PRO_0000355069
Sites
	Active site	85	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8G4 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 1F11927445A1CEBC	FASTA	217	25,028
	10 20 30 40 50 60 MVSRFLMAEY RHLTENPSEN FKVSVNENNM TEWDVILKGP ADTLYEGGLF KAKIGFPPNY 70 80 90 100 110 120 PYEPPRLTFT SEMWHPNIYS DGRVCISILH GDNAEEQGMT WSPVQKIDTI LLSVISLLNE 130 140 150 160 170 180 PNTDSPANVD AAKSYRRFVH KEDLESYPME VKRTVKKSLD ECSPEDIEYF KNAASNVPPI 190 200 210 PSDAYEDESE EMEDDTYILT YDDEDEEEEE DEEMDDE

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261363 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC_ASFP4

Accession

Primary (citable) accession number: P0C8G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	March 6, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program