Reviewed,
UniProtKB/Swiss-Prot P0C865 (MK07_RAT)
Last modified
October 13, 2009.
Version 11.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Mitogen-activated protein kinase 7 EC=2.7.11.24 Alternative name(s): Extracellular signal-regulated kinase 5 Short name=ERK-5 Big MAP kinase 1 Short name=BMK1 kinase | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 806 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Enzyme regulation | Activated by tyrosine and threonine phosphorylation. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF) By similarity. |
| Subunit structure | Interacts with MAP2K5. Forms oligomers By similarity. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D. |
| Subcellular location | Cytoplasm. Nucleus. Note: Translocates to the nucleus upon activation By similarity. |
| Domain | The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. |
| Post-translational modification | Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell cycle Differentiation |
| Cellular component | Cytoplasm Nucleus |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiationInferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 806 | 806 | Mitogen-activated protein kinase 7 | PRO_0000349105 | |||||
Regions | |||||||||
| Domain | 55 – 347 | 293 | Protein kinase | ||||||
| Nucleotide binding | 61 – 69 | 9 | ATP By similarity | ||||||
| Region | 1 – 77 | 77 | Required for cytoplasmic targeting By similarity | ||||||
| Region | 78 – 139 | 62 | Required for binding to MAP2K5 By similarity | ||||||
| Region | 140 – 406 | 267 | Necessary for oligomerization By similarity | ||||||
| Region | 407 – 806 | 400 | May not be required for kinase activity; required to stimulate MEF2C activity By similarity | ||||||
| Region | 505 – 539 | 35 | Nuclear localization signal By similarity | ||||||
| Motif | 219 – 221 | 3 | TXY By similarity | ||||||
| Compositional bias | 403 – 465 | 63 | Pro-rich | ||||||
| Compositional bias | 513 – 543 | 31 | Arg-rich | ||||||
| Compositional bias | 578 – 692 | 115 | Pro-rich | ||||||
Sites | |||||||||
| Active site | 182 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 84 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 219 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 221 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 710 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 723 | 1 | Phosphothreonine By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. Collins F.S.Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway. |
| [2] | "Interaction of myocyte enhancer factor 2 (MEF2) with a mitogen-activated protein kinase, ERK5/BMK1." Yang C.-C., Ornatsky O.I., McDermott J.C., Cruz T.F., Prody C.A. Nucleic Acids Res. 26:4771-4777(1998) [PubMed: 9753748] [Abstract] Cited for: FUNCTION, INTERACTION WITH MEF2A; MEF2C AND MEF2D. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AABR03073216 Genomic DNA. No translation available. | |
| IPI | IPI00209365. |
| UniGene | Rn.144629 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P0C865. |
PTM databases | |
| PhosphoSite | P0C865. |
Organism-specific databases | |
| RGD | 621505. Mapk7. |
Gene expression databases | |
| Genevestigator | P0C865. |
Family and domain databases | |
| InterPro | IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MK07_RAT | ||||||||
| Accession | Primary (citable) accession number: P0C865 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


