Reviewed, UniProtKB/Swiss-Prot P0C7U2 (ASA2C_HUMAN)

Names and origin

Protein names

Recommended name:
    Putative neutral ceramidase C
    EC=3.5.1.23
Alternative name(s):
    N-acylsphingosine amidohydrolase 2C
    Non-lysosomal ceramidase C

Gene names

Name:

ASAH2C

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May hydrolyze the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Sequence similarities	Belongs to the neutral ceramidase family.
Caution	In contrast to other mammalian members of the family, ASAH2C has no predicted transmembrane domain.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Putative neutral ceramidase C		PRO_0000343742
Regions
	Region	612 – 622	11	Required for correct folding and localization By similarity
Sites
	Active site	196	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C7U2-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 2FB96681179A7D4C	FASTA	622	68,749
	10 20 30 40 50 60 MDSEKSSEWR SDVLNRLQSK YGSLYRRDNV ILSGTHTHSG PAGYFQYTVF VIASEGFSNQ 70 80 90 100 110 120 TFQHMVTGIL KSIDIAHTNM KPGKIFINKG NVDGVQINRS PYSYLQNPQS ERARYSSNTD 130 140 150 160 170 180 KEMIVLKMVD LNGDDLGLIS WFAIHPVSMN NSNHLVNSDN VGYASYLLEQ EKNKGYLPGQ 190 200 210 220 230 240 GPFVAAFSSS NLGDVSPNIL GPRCINTGES CDNANSTCPI GGPSMCIAKG PGQDMFDSTQ 250 260 270 280 290 300 IIGRAMYQRA KELYASASQE VTGPLASAHQ WVDMTDVTVW LNSTHASKTC KPALGYSFAA 310 320 330 340 350 360 GTIDGVGGLN FTQGKTEGDP FWDTIRDQIL GKPSEEIKEC HKPKPILLHT GELSKPHPWH 370 380 390 400 410 420 PDIVDVQIIT LGSLAITAIP GEFTTMSGRR LREAVQAEFA SHGMQNMTVV ISGLCNVYTH 430 440 450 460 470 480 YITTYEEYQA QRYEAASTIY GPHTLSAYIQ LFRNLAKAIA TDTVANLSRG PEPPFFKQLI 490 500 510 520 530 540 VPLIPSIVDR APKGRTFGDV LQPAKPEYRV GEVAEVIFVG ANPKNSVQNQ NHQTFLTVEK 550 560 570 580 590 600 YEATSTSWQI VCNDASWETR FYWHKGLLGL SNATVEWHIP DTAQPGIYRI RYFGHNRKQD 610 620 ILKPAVILSF EGTSPAFEVV TI

References

[1]

"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL954360 Genomic DNA. No translation available.
IPI	IPI00472292.
RefSeq	XP_932179.1.
UniGene	Hs.537110
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P0C7U2.
Proteomic databases
PRIDE	P0C7U2.
Genome annotation databases
Ensembl	ENST00000420079; ENSP00000392320; ENSG00000072444; Homo sapiens. [Genome view] ENST00000426610; ENSP00000399947; ENSG00000072444; Homo sapiens. [Genome view]
GeneID	653365.
KEGG	hsa:653365.
Organism-specific databases
CTD	653365.
GeneCards	GC10M047523.
HGNC	HGNC:23457. ASAH2C.
GenAtlas	Search...
Gene expression databases
CleanEx	HS_ASAH2C.
Genevestigator	P0C7U2.
Family and domain databases
InterPro	IPR006823. Ceramidase_alk. [Graphical view]
PANTHER	PTHR12670. Ceramidase_alk. 1 hit.
Pfam	PF04734. Ceramidase_alk. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASA2C_HUMAN

Accession

Primary (citable) accession number: P0C7U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	October 13, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.