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Protein

RWD domain-containing protein 3

Gene

Rwdd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Positively regulates the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Negatively regulates the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Has no effect on ubiquitination.By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).

Names & Taxonomyi

Protein namesi
Recommended name:
RWD domain-containing protein 3
Alternative name(s):
RWD domain-containing sumoylation enhancer
Short name:
RSUME1 Publication
Gene namesi
Name:Rwdd3
Synonyms:Rsume
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi620472. Rwdd3.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Colocalizes with UBC9/UBE2I in nuclear spots.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003400851 – 267RWD domain-containing protein 3Add BLAST267

Proteomic databases

PaxDbiP0C7N0.
PRIDEiP0C7N0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000029893.
GenevisibleiP0C7N0. RN.

Interactioni

Subunit structurei

Interacts with UBE2I/UBC9, NFKBIA, HIF1A and NCOA2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039966.

Structurei

3D structure databases

ProteinModelPortaliP0C7N0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 114RWDPROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15Interaction with UBE2I/UBC9By similarity3
Regioni100 – 102Interaction with UBE2I/UBC9By similarity3

Domaini

The RWD domain is required for the sumoylation enhancement activity.By similarity

Sequence similaritiesi

Contains 1 RWD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGD3. Eukaryota.
ENOG4111KZ9. LUCA.
GeneTreeiENSGT00390000000954.
HOGENOMiHOG000133077.
HOVERGENiHBG057524.
InParanoidiP0C7N0.
OMAiQLTRAQC.
OrthoDBiEOG091G0OZC.
PhylomeDBiP0C7N0.
TreeFamiTF324344.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C7N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEMRQELS ALAAIFCGPH EWEMLSCSET DGAVFRIHTT AEGLAGEDVP
60 70 80 90 100
LELAFHLPAG YPSCLPGISV NSERLTRAQC VTVKEKLLGE ARRLLSEPMV
110 120 130 140 150
HELVLWTQQN LRHILSQTET ESSNGTCTLP ESSTVDGGLW MTLLRLDHMR
160 170 180 190 200
ARTKYVKVVE KWASELRLTG RLMFMGKMIL ILLQGDRSNI KEYLILQKTS
210 220 230 240 250
KVDVDSSGKK CKEKMISVLS ETKVQTEHKR FLAFEVKEYS TLEELQKEFE
260
TAGLQELFSE CVLGLVK
Length:267
Mass (Da):30,138
Last modified:June 10, 2008 - v1
Checksum:i764BFB3384E6407A
GO

Sequence databases

RefSeqiNP_001121624.1. NM_001128152.1.
UniGeneiRn.54470.

Genome annotation databases

EnsembliENSRNOT00000040080; ENSRNOP00000039966; ENSRNOG00000029893.
GeneIDi65026.
KEGGirno:65026.
UCSCiRGD:620472. rat.

Cross-referencesi

Sequence databases

RefSeqiNP_001121624.1. NM_001128152.1.
UniGeneiRn.54470.

3D structure databases

ProteinModelPortaliP0C7N0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039966.

Proteomic databases

PaxDbiP0C7N0.
PRIDEiP0C7N0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040080; ENSRNOP00000039966; ENSRNOG00000029893.
GeneIDi65026.
KEGGirno:65026.
UCSCiRGD:620472. rat.

Organism-specific databases

CTDi25950.
RGDi620472. Rwdd3.

Phylogenomic databases

eggNOGiENOG410IGD3. Eukaryota.
ENOG4111KZ9. LUCA.
GeneTreeiENSGT00390000000954.
HOGENOMiHOG000133077.
HOVERGENiHBG057524.
InParanoidiP0C7N0.
OMAiQLTRAQC.
OrthoDBiEOG091G0OZC.
PhylomeDBiP0C7N0.
TreeFamiTF324344.

Enzyme and pathway databases

ReactomeiR-RNO-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).

Miscellaneous databases

PROiP0C7N0.

Gene expression databases

BgeeiENSRNOG00000029893.
GenevisibleiP0C7N0. RN.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRWDD3_RAT
AccessioniPrimary (citable) accession number: P0C7N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.