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Protein

3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase

Gene

paaJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the thiolytic cleavage of the beta-keto C8 intermediate 3-oxo-5,6-dehydrosuberyl-CoA with CoA to yield the C6 intermediate 2,3-dehydroadipyl-CoA and acetyl-CoA. Besides it catalyzes also the last step of the pathway, in which 3-oxoadipyl-CoA similarly is cleaved to acetyl-CoA and succinyl-CoA.3 Publications

Catalytic activityi

Succinyl-CoA + acetyl-CoA = CoA + 3-oxoadipyl-CoA.2 Publications
2,3-didehydroadipyl-CoA + acetyl-CoA = CoA + 3-oxo-5,6-didehydrosuberyl-CoA.2 Publications

Pathwayi: phenylacetate degradation

This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Acyl-thioester intermediateBy similarity1
Active sitei357Proton acceptorPROSITE-ProRule annotation1
Active sitei387Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • 3-oxoadipyl-CoA thiolase activity Source: UniProtKB
  • transferase activity Source: EcoCyc

GO - Biological processi

  • 3,4-dihydroxybenzoate catabolic process Source: InterPro
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • phenylacetate catabolic process Source: UniProtKB

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6718-MONOMER
MetaCyc:G6718-MONOMER
UniPathwayiUPA00930

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase (EC:2.3.1.1742 Publications, EC:2.3.1.2232 Publications)
Gene namesi
Name:paaJ
Synonyms:paaE, ydbW
Ordered Locus Names:b1397, JW1392
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13744 paaJ

Pathology & Biotechi

Disruption phenotypei

Mutants are unable to use phenylacetate as a carbon source.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002064211 – 4013-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolaseAdd BLAST401

Proteomic databases

PaxDbiP0C7L2
PRIDEiP0C7L2

Expressioni

Inductioni

Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4263012239 interactors.
IntActiP0C7L2 5 interactors.
STRINGi316385.ECDH10B_1522

Structurei

3D structure databases

ProteinModelPortaliP0C7L2
SMRiP0C7L2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Phylogenomic databases

eggNOGiENOG4105CHU Bacteria
COG0183 LUCA
HOGENOMiHOG000012239
InParanoidiP0C7L2
KOiK02615
OMAiFAYCVEE
PhylomeDBiP0C7L2

Family and domain databases

CDDicd00751 thiolase, 1 hit
Gene3Di3.40.47.104 hits
InterProiView protein in InterPro
IPR012793 PcaF
IPR002155 Thiolase
IPR016039 Thiolase-like
IPR020615 Thiolase_acyl_enz_int_AS
IPR020610 Thiolase_AS
IPR020617 Thiolase_C
IPR020613 Thiolase_CS
IPR020616 Thiolase_N
PfamiView protein in Pfam
PF02803 Thiolase_C, 1 hit
PF00108 Thiolase_N, 1 hit
PIRSFiPIRSF000429 Ac-CoA_Ac_transf, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
TIGR02430 pcaF, 1 hit
PROSITEiView protein in PROSITE
PS00098 THIOLASE_1, 1 hit
PS00737 THIOLASE_2, 1 hit
PS00099 THIOLASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P0C7L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREAFICDGI RTPIGRYGGA LSSVRADDLA AIPLRELLVR NPRLDAECID
60 70 80 90 100
DVILGCANQA GEDNRNVARM ATLLAGLPQS VSGTTINRLC GSGLDALGFA
110 120 130 140 150
ARAIKAGDGD LLIAGGVESM SRAPFVMGKA ASAFSRQAEM FDTTIGWRFV
160 170 180 190 200
NPLMAQQFGT DSMPETAENV AELLKISRED QDSFALRSQQ RTAKAQSSGI
210 220 230 240 250
LAEEIVPVVL KNKKGVVTEI QHDEHLRPET TLEQLRGLKA PFRANGVITA
260 270 280 290 300
GNASGVNDGA AALIIASEQM AAAQGLTPRA RIVAMATAGV EPRLMGLGPV
310 320 330 340 350
PATRRVLERA GLSIHDMDVI ELNEAFAAQA LGVLRELGLP DDAPHVNPNG
360 370 380 390 400
GAIALGHPLG MSGARLALAA SHELHRRNGR YALCTMCIGV GQGIAMILER

V
Length:401
Mass (Da):42,277
Last modified:June 10, 2008 - v1
Checksum:i6B8C26ECB4CFEC00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74479.1
AP009048 Genomic DNA Translation: BAA15002.1
PIRiH64890
RefSeqiNP_415915.1, NC_000913.3
WP_001206197.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74479; AAC74479; b1397
BAA15002; BAA15002; BAA15002
GeneIDi946121
KEGGiecj:JW1392
eco:b1397
PATRICifig|1411691.4.peg.874

Similar proteinsi

Entry informationi

Entry nameiPAAJ_ECOLI
AccessioniPrimary (citable) accession number: P0C7L2
Secondary accession number(s): O53017, P77525
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome