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Protein

WAS/WASL-interacting protein family member 3

Gene

Wipf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a regulator of cytoskeletal organization (Potential). May have a role in spermatogenesis.Curated1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
WAS/WASL-interacting protein family member 3
Alternative name(s):
Corticosteroids and regional expression protein 16 homolog
Gene namesi
Name:Wipf3
Synonyms:Cr16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:3044681. Wipf3.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: In hippocampal neurons colocalizes with WASL in the cell body, axons and the growth cone (By similarity). Localizes to the actin filaments at the Sertoli cell-spermatid junctions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are normal but males are sterile due to defects in spermatogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485WAS/WASL-interacting protein family member 3PRO_0000337998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei161 – 1611PhosphoserineCombined sources
Modified residuei211 – 2111PhosphoserineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0C7L0.
PaxDbiP0C7L0.
PRIDEiP0C7L0.

PTM databases

iPTMnetiP0C7L0.
PhosphoSiteiP0C7L0.

Expressioni

Tissue specificityi

Isoform 1 is expressed in brain and testis and isoform 2 is expressed only in brain (at protein level).1 Publication

Interactioni

Subunit structurei

Interacts with WASL, and monomeric and filamentous actin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120240.

Structurei

3D structure databases

ProteinModelPortaliP0C7L0.
SMRiP0C7L0. Positions 419-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 7318WH2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi3 – 86Profilin-binding motif
Motifi11 – 166Profilin-binding motif
Motifi31 – 366Profilin-binding motif
Motifi69 – 724RLRK
Motifi424 – 44825WASP-binding motifAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi184 – 20118Poly-ProAdd
BLAST
Compositional biasi232 – 24615Poly-ProAdd
BLAST
Compositional biasi296 – 31217Poly-ProAdd
BLAST

Domaini

The WH2 domain is found in a number of putative actin-binding proteins.By similarity
The profilin-binding motif has been implicated in the interaction with profilin and SH3 domains.By similarity
The KLKR motif is essential for G-actin binding and for actin polymerization.By similarity

Sequence similaritiesi

Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J09R. Eukaryota.
ENOG410Y0AH. LUCA.
HOGENOMiHOG000090003.
HOVERGENiHBG108677.
InParanoidiP0C7L0.
PhylomeDBiP0C7L0.

Family and domain databases

InterProiIPR003124. WH2_dom.
[Graphical view]
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P0C7L0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVPPPPPPP PPPPPPPPPP LGAPPPPPLG APPPPPPPGP PVSTDTPSLR
60 70 80 90 100
KPDLKGRSAL LADIQQGTRL RKVTQINDRS APQIEGSKGT SKEGGAAGSN
110 120 130 140 150
ARGGNTPPAL GDLFAGGFPV LRPAGQRDVS GGKSGQGPGS RAPSPRLPIK
160 170 180 190 200
AISGPLPAPA SPRLGNASET HSSARPVPPR PSVPAPPPPT PPPPPPPPLP
210 220 230 240 250
PASPIKAQLV SPPAPPTKVN PSVVPPPLPC APPLPPPPPT PPPLPPASAL
260 270 280 290 300
SDKAVRPQLA PLHLPPIPPP LPLLPPCGYP GLHSEPNSPA QEVREPPAPP
310 320 330 340 350
PPPPPPPPPP LPTYASCSSR TAVAPPPLPG ANNSGSETPP PLPPKSPSFQ
360 370 380 390 400
TQKALPTPPG APGPQAILQK KRRGPGTSGG KLNPPPAPPA RSPTTELSSK
410 420 430 440 450
SQQPGGQLRN GGQHAIDDFE SKFTFHSMED FPPPDEYKPC QKIYPSKVPR
460 470 480
SRTPGSWLQA EAAGQSSDDI KSRNSQLSLK ALPVR
Length:485
Mass (Da):49,453
Last modified:June 10, 2008 - v1
Checksum:i1ACA37E855D40447
GO
Isoform 2 (identifier: P0C7L0-2) [UniParc]FASTAAdd to basket

Also known as: deltains

The sequence of this isoform differs from the canonical sequence as follows:
     417-451: DDFESKFTFHSMEDFPPPDEYKPCQKIYPSKVPRS → G

Show »
Length:451
Mass (Da):45,334
Checksum:i1E61F3B3E4769E20
GO

Sequence cautioni

The sequence CB723002 differs from that shown. Reason: Frameshift at position 621. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti482 – 4821L → LPV (PubMed:17573773).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei417 – 45135DDFES…KVPRS → G in isoform 2. 1 PublicationVSP_034030Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087841 Genomic DNA. No translation available.
BQ952480 mRNA. No translation available.
CB723002 mRNA. No translation available.
UniGeneiMm.434326.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087841 Genomic DNA. No translation available.
BQ952480 mRNA. No translation available.
CB723002 mRNA. No translation available.
UniGeneiMm.434326.

3D structure databases

ProteinModelPortaliP0C7L0.
SMRiP0C7L0. Positions 419-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120240.

PTM databases

iPTMnetiP0C7L0.
PhosphoSiteiP0C7L0.

Proteomic databases

MaxQBiP0C7L0.
PaxDbiP0C7L0.
PRIDEiP0C7L0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:3044681. Wipf3.

Phylogenomic databases

eggNOGiENOG410J09R. Eukaryota.
ENOG410Y0AH. LUCA.
HOGENOMiHOG000090003.
HOVERGENiHBG108677.
InParanoidiP0C7L0.
PhylomeDBiP0C7L0.

Miscellaneous databases

ChiTaRSiWipf3. mouse.
PROiP0C7L0.
SOURCEiSearch...

Family and domain databases

InterProiIPR003124. WH2_dom.
[Graphical view]
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-68 AND 203-485.
    Tissue: Brain and Mammary tumor.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart and Testis.

Entry informationi

Entry nameiWIPF3_MOUSE
AccessioniPrimary (citable) accession number: P0C7L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 6, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.