Reviewed, UniProtKB/Swiss-Prot P0C7J3 (XANB_XANCP)

Names and origin

Protein names

Recommended name:
    Xanthan biosynthesis protein xanB
Including the following 2 domains:
    1- Recommended name:
            Mannose-6-phosphate isomerase
              EC=5.3.1.8
        Alternative name(s):
            Phosphomannose isomerase
              Short name=PMI
            Phosphohexomutase
    2- Recommended name:
            Mannose-1-phosphate guanylyl transferase
              EC=2.7.7.13
        Alternative name(s):
            GDP-mannose pyrophosphorylase
              Short name=GMPP
              Short name=GMP

Gene names

Name:	xanB
Ordered Locus Names:	XCC0625

Organism

Xanthomonas campestris pv. campestris [Complete proteome] [HAMAP]

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in xanthan production By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate. GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Exopolysaccharide synthesis Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Isomerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Xanthan biosynthesis protein xanB		PRO_0000194252

Sequences

	Sequence		Length	Mass (Da)
	P0C7J3-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 77C8B346D7F25E3B	FASTA	467	50,962
	10 20 30 40 50 60 MSDVLPIILS GGSGTRLWPL SRESYPKQFL PLVGEHSMLQ ATWLRSAPVA AHAPIVVANE 70 80 90 100 110 120 EHRFMAAEQL QQLGVKPSAI LLEPKGRNTA PAIAVAALEA TRNGGDPLLL VLPSDHVIRD 130 140 150 160 170 180 EAAFQAAVTV AAAAAEQGKL VTFGIKPTAP ETGYGYIKAG VGTGATAVER FVEKPDLATA 190 200 210 220 230 240 QGYLASGEYY WNSGMFLFRA SRYLEELRKF QPAIADACQK AWEGGKRDAD FTRLDKDAFA 250 260 270 280 290 300 SSPSDSIDYA VMEKTADAVV VPLDAGWNDV GSWSSLLDVS EQDGQGNAHH GDVIQLDCKN 310 320 330 340 350 360 TYAYGSRLIA MVGLENVVVV ETDDAVLVGH RDRIQEVKEV VSQIKSAGRS EATWHRKVYR 370 380 390 400 410 420 PWGAYDSIDM GQRFQVKRIT VKPGATLSLQ MHHHRAEHWI VVSGTAEVTR GEEVLLLTEN 430 440 450 460 QSTYIPLGVT HRLKNPGKLP LELIEVQSGS YLGEDDIVRF EDTYGRT

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008922 Genomic DNA. Translation: AAM39941.1.
RefSeq	NP_636017.1.
3D structure databases
SMR	P0C7J3. Positions 4-269, 356-466.
ModBase	Search...
Genome annotation databases
GeneID	1000899.
GenomeReviews	Gene locus XCC0625 in contig AE008922_GR.
KEGG	xcc:XCC0625.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG422488.
OMA	AEHRMVA.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

XANB_XANCP

Accession

Primary (citable) accession number: P0C7J3
Secondary accession number(s): P29956, Q93S98

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)