P0C7J3 (XANB_XANCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthan biosynthesis protein XanB

Including the following 2 domains:

1. Mannose-6-phosphate isomerase
   EC=5.3.1.8
   Alternative name(s):
   Phosphohexomutase
   Phosphomannose isomerase
   Short name=PMI
2. Mannose-1-phosphate guanylyl transferase
   EC=2.7.7.13
   Alternative name(s):
   GDP-mannose pyrophosphorylase
   Short name=GMP
   Short name=GMPP

Gene names

Name:	xanB
Ordered Locus Names:	XCC0625

Organism

Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568) [Reference proteome] [HAMAP]

Taxonomic identifier

190485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas campestris › Xanthomonas campestris pv. campestris

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in xanthan production By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate. GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Exopolysaccharide synthesis Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Isomerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Xanthan biosynthesis protein XanB		PRO_0000194252

Sequences

	Sequence		Length	Mass (Da)
	P0C7J3 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 77C8B346D7F25E3B	FASTA	467	50,962
	10 20 30 40 50 60 MSDVLPIILS GGSGTRLWPL SRESYPKQFL PLVGEHSMLQ ATWLRSAPVA AHAPIVVANE 70 80 90 100 110 120 EHRFMAAEQL QQLGVKPSAI LLEPKGRNTA PAIAVAALEA TRNGGDPLLL VLPSDHVIRD 130 140 150 160 170 180 EAAFQAAVTV AAAAAEQGKL VTFGIKPTAP ETGYGYIKAG VGTGATAVER FVEKPDLATA 190 200 210 220 230 240 QGYLASGEYY WNSGMFLFRA SRYLEELRKF QPAIADACQK AWEGGKRDAD FTRLDKDAFA 250 260 270 280 290 300 SSPSDSIDYA VMEKTADAVV VPLDAGWNDV GSWSSLLDVS EQDGQGNAHH GDVIQLDCKN 310 320 330 340 350 360 TYAYGSRLIA MVGLENVVVV ETDDAVLVGH RDRIQEVKEV VSQIKSAGRS EATWHRKVYR 370 380 390 400 410 420 PWGAYDSIDM GQRFQVKRIT VKPGATLSLQ MHHHRAEHWI VVSGTAEVTR GEEVLLLTEN 430 440 450 460 QSTYIPLGVT HRLKNPGKLP LELIEVQSGS YLGEDDIVRF EDTYGRT

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008922 Genomic DNA. Translation: AAM39941.1.
RefSeq	NP_636017.1. NC_003902.1.
3D structure databases
ProteinModelPortal	P0C7J3.
ModBase	Search...
Protein-protein interaction databases
STRING	190485.XCC0625.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM39941; AAM39941; XCC0625.
GeneID	1000899.
KEGG	xcc:XCC0625.
PATRIC	24071902. VBIXanCam115730_0686.
Phylogenomic databases
eggNOG	COG0662.
HOGENOM	HOG000153549.
KO	K16011.
OMA	HHEALER.
ProtClustDB	CLSK445961.
Enzyme and pathway databases
UniPathway	UPA00126; UER00423. UPA00126; UER00930.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

XANB_XANCP

Accession

Primary (citable) accession number: P0C7J3
Secondary accession number(s): P29956, Q93S98

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program