P0C7B7 (ELIC_ERWCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cys-loop ligand-gated ion channel Alternative name(s): ELIC
Organism	Erwinia chrysanthemi
Taxonomic identifier	556 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates cation-selective currents but does not discriminate between different monovalent cations. Ref.1
Subunit structure	Homopentamer. Ref.1
Subcellular location	Cell inner membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
Technical term	3D-structure
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 321

321

Cys-loop ligand-gated ion channel

PRO_0000330947

Regions

Topological domain

1 – 203

203

Periplasmic Potential

Transmembrane

204 – 224

21

Helical; Potential

Topological domain

225 – 232

8

Cytoplasmic Potential

Transmembrane

233 – 253

21

Helical; Potential

Topological domain

254 – 263

10

Periplasmic Potential

Transmembrane

264 – 284

21

Helical; Potential

Topological domain

285 – 300

16

Cytoplasmic Potential

Transmembrane

301 – 321

21

Helical; Potential

Motif

115 – 123

9

Cys-loop

Secondary structure

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321

Helix Strand Turn

Details...

Beta strand

12 – 27

16

Turn

28 – 31

4

Beta strand

32 – 43

12

Beta strand

51 – 54

4

Beta strand

56 – 58

3

Helix

60 – 68

9

Beta strand

76 – 78

3

Beta strand

81 – 94

14

Beta strand

100 – 111

12

Helix

119 – 121

3

Beta strand

123 – 134

12

Beta strand

136 – 145

10

Turn

155 – 157

3

Beta strand

158 – 161

4

Beta strand

169 – 173

5

Helix

178 – 180

3

Beta strand

186 – 198

13

Helix

201 – 206

6

Helix

208 – 219

12

Turn

220 – 223

4

Helix

227 – 251

25

Helix

260 – 282

23

Beta strand

285 – 290

6

Turn

294 – 298

5

Turn

300 – 302

3

Beta strand

303 – 305

3

Helix

308 – 311

4

Beta strand

312 – 314

3

Sequences

	Sequence		Length	Mass (Da)
	P0C7B7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: C532E1C4B657896D	FASTA	321	36,808
	10 20 30 40 50 60 APADNAADAR PVDVSVSIFI NKIYGVNTLE QTYKVDGYIV AQWTGKPRKT PGDKPLIVEN 70 80 90 100 110 120 TQIERWINNG LWVPALEFIN VVGSPDTGNK RLMLFPDGRV IYNARFLGSF SNDMDFRLFP 130 140 150 160 170 180 FDRQQFVLEL EPFSYNNQQL RFSDIQVYTE NIDNEEIDEW WIRKASTHIS DIRYDHLSSV 190 200 210 220 230 240 QPNQNEFSRI TVRIDAVRNP SYYLWSFILP LGLIIAASWS VFWLESFSER LQTSFTLMLT 250 260 270 280 290 300 VVAYAFYTSN ILPRLPYTTV IDQMIIAGYG SIFAAILLII FAHHRQAMGV EDDLLIQRCR 310 320 LAFPLGFLAI GCVLVIRGIT L

References

[1]	"X-ray structure of a prokaryotic pentameric ligand-gated ion channel." Hilf R.J., Dutzler R. Nature 452:375-379(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VL0	X-ray	3.30	A/B/C/D/E/F/G/H/I/J	1-321	[»]
2YKS	X-ray	3.30	A/B/C/D/E/F/G/H/I/J	1-321	[»]
2YOE	X-ray	3.90	A/B/C/D/E/F/G/H/I/J	11-316	[»]
3ZKR	X-ray	3.65	A/B/C/D/E/F/G/H/I/J	11-316	[»]
4A97	X-ray	3.34	A/B/C/D/E/F/G/H/I/J	11-316	[»]
4A98	X-ray	3.61	A/B/C/D/E/F/G/H/I/J	11-316	[»]

ProteinModelPortal

P0C7B7.

SMR

P0C7B7. Positions 11-316.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-58973N.

Protein family/group databases

TCDB

1.A.9.9.1. neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.70.170.10. 1 hit.

InterPro

IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
[Graphical view]

PANTHER

PTHR18945. PTHR18945. 1 hit.

Pfam

PF02931. Neur_chan_LBD. 1 hit.
[Graphical view]

SUPFAM

SSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0C7B7.

Entry information

Entry name

ELIC_ERWCH

Accession

Primary (citable) accession number: P0C7B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	April 3, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program