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Protein

Ghrelin O-acyltransferase

Gene

Mboat4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei307 – 3071Curated
Active sitei338 – 3381Curated

GO - Molecular functioni

  • serine O-acyltransferase activity Source: UniProtKB

GO - Biological processi

  • peptidyl-serine octanoylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-422085. Synthesis, secretion, and deacylation of Ghrelin.

Names & Taxonomyi

Protein namesi
Recommended name:
Ghrelin O-acyltransferase (EC:2.3.1.-)
Alternative name(s):
Membrane-bound O-acyltransferase domain-containing protein 4
Short name:
O-acyltransferase domain-containing protein 4
Gene namesi
Name:Mboat4
Synonyms:Gm171, Goat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2685017. Mboat4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Transmembranei45 – 6521HelicalSequence analysisAdd
BLAST
Transmembranei163 – 18321HelicalSequence analysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence analysisAdd
BLAST
Transmembranei331 – 35121HelicalSequence analysisAdd
BLAST
Transmembranei377 – 39721HelicalSequence analysisAdd
BLAST
Transmembranei408 – 42821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display a complete lack of octanoylated ghrelin in blood.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi181 – 1811C → A: No effect on ability to acylate ghrelin. 1 Publication
Mutagenesisi307 – 3071N → A: Abolished ability to acylate ghrelin. 1 Publication
Mutagenesisi338 – 3381H → A: Abolished ability to acylate ghrelin. 1 Publication

Chemistry

ChEMBLiCHEMBL1255134.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Ghrelin O-acyltransferasePRO_0000329073Add
BLAST

Proteomic databases

PaxDbiP0C7A3.
PRIDEiP0C7A3.

PTM databases

iPTMnetiP0C7A3.
PhosphoSiteiP0C7A3.

Expressioni

Tissue specificityi

Highly expressed in stomach. Lower expression in small intestine and colon. Very low expression in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000071113.
GenevisibleiP0C7A3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092988.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2704. Eukaryota.
COG5202. LUCA.
GeneTreeiENSGT00550000074565.
HOGENOMiHOG000113492.
HOVERGENiHBG108120.
InParanoidiP0C7A3.
OMAiAFSAWWH.
OrthoDBiEOG091G07L0.
PhylomeDBiP0C7A3.
TreeFamiTF314906.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C7A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWLQLFFLH PLSFYQGAAF PFALLFNYLC ILDTFSTRAR YLFLLAGGGV
60 70 80 90 100
LAFAAMGPYS LLIFIPALCA VALVSFLSPQ EVHRLTFFFQ MGWQTLCHLG
110 120 130 140 150
LHYTEYYLGE PPPVRFYITL SSLMLLTQRV TSLSLDICEG KVEAPRRGIR
160 170 180 190 200
SKSSFSEHLW DALPHFSYLL FFPALLGGSL CSFRRFQACV QRSSSLYPSI
210 220 230 240 250
SFRALTWRGL QILGLECLKV ALRSAVSAGA GLDDCQRLEC IYLMWSTAWL
260 270 280 290 300
FKLTYYSHWI LDDSLLHAAG FGAEAGQGPG EEGYVPDVDI WTLETTHRIS
310 320 330 340 350
LFARQWNRST ALWLRRLVFR KSRRWPLLQT FAFSAWWHGL HPGQVFGFLC
360 370 380 390 400
WSVMVKADYL IHTFANVCIR SWPLRLLYRA LTWAHTQLII AYIMLAVEGR
410 420 430
SLSSLCQLCC SYNSLFPVMY GLLLFLLAER KDKRN
Length:435
Mass (Da):49,879
Last modified:April 8, 2008 - v1
Checksum:iC2B7E69E35185620
GO

Sequence cautioni

The sequence AAI47845 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAI47850 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311I → V in AAI47845 (PubMed:15489334).Curated
Sequence conflicti31 – 311I → V in AAI47850 (PubMed:15489334).Curated
Sequence conflicti53 – 531F → L in AAI47845 (PubMed:15489334).Curated
Sequence conflicti53 – 531F → L in AAI47850 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU721729 mRNA. Translation: ACD93144.1.
EU518496 mRNA. Translation: ACB05874.1.
AC167537 Genomic DNA. No translation available.
BC147844 mRNA. Translation: AAI47845.1. Different initiation.
BC147849 mRNA. Translation: AAI47850.1. Different initiation.
CCDSiCCDS52540.1.
RefSeqiNP_001119786.1. NM_001126314.2.
UniGeneiMm.324008.

Genome annotation databases

EnsembliENSMUST00000095345; ENSMUSP00000092988; ENSMUSG00000071113.
GeneIDi234155.
KEGGimmu:234155.
UCSCiuc012gch.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU721729 mRNA. Translation: ACD93144.1.
EU518496 mRNA. Translation: ACB05874.1.
AC167537 Genomic DNA. No translation available.
BC147844 mRNA. Translation: AAI47845.1. Different initiation.
BC147849 mRNA. Translation: AAI47850.1. Different initiation.
CCDSiCCDS52540.1.
RefSeqiNP_001119786.1. NM_001126314.2.
UniGeneiMm.324008.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092988.

Chemistry

ChEMBLiCHEMBL1255134.

PTM databases

iPTMnetiP0C7A3.
PhosphoSiteiP0C7A3.

Proteomic databases

PaxDbiP0C7A3.
PRIDEiP0C7A3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095345; ENSMUSP00000092988; ENSMUSG00000071113.
GeneIDi234155.
KEGGimmu:234155.
UCSCiuc012gch.1. mouse.

Organism-specific databases

CTDi619373.
MGIiMGI:2685017. Mboat4.

Phylogenomic databases

eggNOGiKOG2704. Eukaryota.
COG5202. LUCA.
GeneTreeiENSGT00550000074565.
HOGENOMiHOG000113492.
HOVERGENiHBG108120.
InParanoidiP0C7A3.
OMAiAFSAWWH.
OrthoDBiEOG091G07L0.
PhylomeDBiP0C7A3.
TreeFamiTF314906.

Enzyme and pathway databases

ReactomeiR-MMU-422085. Synthesis, secretion, and deacylation of Ghrelin.

Miscellaneous databases

PROiP0C7A3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071113.
GenevisibleiP0C7A3. MM.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
[Graphical view]
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBOA4_MOUSE
AccessioniPrimary (citable) accession number: P0C7A3
Secondary accession number(s): B1Q004, B2RWL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: September 7, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.