P0C7A1 (ENASE_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic endo-beta-N-acetylglucosaminidase
Short name=ENGase
EC=3.2.1.96

Gene names

Name:

ENGASE

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
Catalytic activity	Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
Subcellular location	Cytoplasm › cytosol Probable Ref.2.
Sequence similarities	Belongs to the glycosyl hydrolase 85 family. Contains 1 BRCT domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Cytosolic endo-beta-N-acetylglucosaminidase		PRO_0000328869
Regions
	Domain	287 – 381	95	BRCT

Sequences

	Sequence		Length	Mass (Da)
	P0C7A1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: CAA311512C095918	FASTA	728	80,889
	10 20 30 40 50 60 MQSQSVMLEL REQDEVWVRL YKGERENAVF SDEYDTYITF SGHLINFQPA AEPLGTTVLH 70 80 90 100 110 120 AAVDTRPQPA RYFDTGTTEP VSFFLSGLEE LLAWHPSSDD EFNVCAVPLA QRQPPLHSRR 130 140 150 160 170 180 PRTLLCHDMR GGYLEDRFIQ GSATRNPYVF YHWRYVDIFV YFSHHTVTIP PVCWTNAAHR 190 200 210 220 230 240 NGVPVLGTFI TEWADGEKLC EAFLAGGEDA YRAVSHQLAR IAQHYRFDGW LINIENALSA 250 260 270 280 290 300 AAVGNLSPFL RHLTAEVHGA VPGGLVIWYD SILESGTLRW QNELNQQNRV FFDACDGLFV 310 320 330 340 350 360 NYNWKEEHLE RTRELAGQRH ADVYIGVDVF ARGDVVGGGF DTNKSLSLIR KHGLSAAIFA 370 380 390 400 410 420 PGWVYKHLGE ENFLLNEDKF WGLLEDYLPT HSICTLPLAT SFSVGMGTGM FLAGKEEEAG 430 440 450 460 470 480 PWYNLSAQEI QPLYPERRGW LSTSCCLQDA WCGGSSLRVQ GTIPPGEERV AIRLSLWVDL 490 500 510 520 530 540 GSSGFSLSIC GTLASGPHRD DFTVALELTT WHSSRCHDGT VTVLPSEDEP HGRHHPHLLP 550 560 570 580 590 600 APPPALSRML AACSHGAQGW TSRCYEQELR GCSLRDLSLL VSRQQASPQE TSFSCLLGEL 610 620 630 640 650 660 RVLDAGSMAA SPPQVQSLTA SQLWWQDGPS AEQLSLSLTL RWAFPPGRAA CFRVLSQGAR 670 680 690 700 710 720 CHRAQPAQPQ LLGLAHGCQY RAVGLAVPRP APGQSCQLEL LVEPVLPSEL PVGPERWGRL LLVYSEPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution." Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A. , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K. Nature 432:695-716(2004) [PubMed: 15592404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Red jungle fowl.
[2]	"Endo-beta-N-acetylglucosaminidase, an enzyme involved in processing of free oligosaccharides in the cytosol." Suzuki T., Yano K., Sugimoto S., Kitajima K., Lennarz W.J., Inoue S., Inoue Y., Emori Y. Proc. Natl. Acad. Sci. U.S.A. 99:9691-9696(2002) [PubMed: 12114544] [Abstract] Cited for: PROTEIN SEQUENCE OF 51-76, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
IPI	IPI00587511.
UniGene	Gga.39266.
3D structure databases
ProteinModelPortal	P0C7A1.
ModBase	Search...
Protein-protein interaction databases
STRING	P0C7A1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	veNOG04354.
GeneTree	ENSGT00390000018512.
HOGENOM	HBG446184.
HOVERGEN	HBG107848.
OrthoDB	EOG44J2HF.
Family and domain databases
InterPro	IPR001073. C1q. IPR005201. Glyco_hydro_85. IPR008983. Tumour_necrosis_fac-like. [Graphical view]
Gene3D	G3DSA:2.60.120.40. Tumour_necrosis_fac-like. 1 hit.
Pfam	PF00386. C1q. 1 hit. PF03644. Glyco_hydro_85. 1 hit. [Graphical view]
SUPFAM	SSF49842. TNF_like. 1 hit.
PROSITE	PS50172. BRCT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENASE_CHICK

Accession

Primary (citable) accession number: P0C7A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	September 21, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program