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Protein

Envelope glycoprotein B

Gene

gB

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding to its host receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress.

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein B
Short name:
gB
Gene namesi
Name:gB
ORF Names:BALF4
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007641 Componenti: Genome

Subcellular locationi

  • Virion membrane ; Single-pass type I membrane protein
  • Host cell membrane ; Single-pass type I membrane protein
  • Host endosome membrane ; Single-pass type I membrane protein
  • Host Golgi apparatus membrane ; Single-pass type I membrane protein
  • Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN).

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 732Virion surfaceAdd BLAST711
Transmembranei733 – 753HelicalAdd BLAST21
Topological domaini754 – 857IntravirionAdd BLAST104

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host endosome, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000038546822 – 857Envelope glycoprotein BAdd BLAST836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 528
Disulfide bondi68 ↔ 484
Glycosylationi76N-linked (GlcNAc...) asparagine; by host1
Disulfide bondi141 ↔ 206
Glycosylationi163N-linked (GlcNAc...) asparagine; by host1
Glycosylationi290N-linked (GlcNAc...) asparagine; by host1
Disulfide bondi295 ↔ 342
Glycosylationi329N-linked (GlcNAc...) asparagine; by host1
Glycosylationi348N-linked (GlcNAc...) asparagine; by host1
Glycosylationi395N-linked (GlcNAc...) asparagine; by host1
Glycosylationi436N-linked (GlcNAc...) asparagine; by host1
Disulfide bondi551 ↔ 588
Glycosylationi563N-linked (GlcNAc...) asparagine; by host1
Glycosylationi629N-linked (GlcNAc...) asparagine; by host1

Post-translational modificationi

A proteolytic cleavage by host furin generates two subunits that remain linked by disulfide bonds.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei432 – 433Cleavage; by host furinSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Binds to heparan sulfate proteoglycans. Interacts with gH/gL heterodimer.

Protein-protein interaction databases

IntActiP0C762. 15 interactors.

Structurei

3D structure databases

SMRiP0C762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 114Involved in fusion and/or binding to host membrane7
Regioni192 – 200Involved in fusion and/or binding to host membrane9
Regioni678 – 730Hydrophobic membrane proximal regionAdd BLAST53
Regioni709 – 729Hydrophobic membrane proximal regionAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi402 – 416Pro-richAdd BLAST15
Compositional biasi428 – 432Poly-Arg5
Compositional biasi836 – 839Poly-Arg4

Sequence similaritiesi

Belongs to the herpesviridae glycoprotein B family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900002K.

Family and domain databases

HAMAPiMF_04032. HSV_GB. 1 hit.
InterProiView protein in InterPro
IPR035377. Glycoprot_B_PH1.
IPR035381. Glycoprot_B_PH2.
IPR000234. Herpes_Glycoprot_B.
PfamiView protein in Pfam
PF17416. Glycoprot_B_PH1. 1 hit.
PF17417. Glycoprot_B_PH2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRRRVLSVV VLLAALACRL GAQTPEQPAP PATTVQPTAT RQQTSFPFRV
60 70 80 90 100
CELSSHGDLF RFSSDIQCPS FGTRENHTEG LLMVFKDNII PYSFKVRSYT
110 120 130 140 150
KIVTNILIYN GWYADSVTNR HEEKFSVDSY ETDQMDTIYQ CYNAVKMTKD
160 170 180 190 200
GLTRVYVDRD GVNITVNLKP TGGLANGVRR YASQTELYDA PGWLIWTYRT
210 220 230 240 250
RTTVNCLITD MMAKSNSPFD FFVTTTGQTV EMSPFYDGKN KETFHERADS
260 270 280 290 300
FHVRTNYKIV DYDNRGTNPQ GERRAFLDKG TYTLSWKLEN RTAYCPLQHW
310 320 330 340 350
QTFDSTIATE TGKSIHFVTD EGTSSFVTNT TVGIELPDAF KCIEEQVNKT
360 370 380 390 400
MHEKYEAVQD RYTKGQEAIT YFITSGGLLL AWLPLTPRSL ATVKNLTELT
410 420 430 440 450
TPTSSPPSSP SPPAPPAARG STSAAVLRRR RRDAGNATTP VPPAAPGKSL
460 470 480 490 500
GTLNNPATVQ IQFAYDSLRR QINRMLGDLA RAWCLEQKRQ NMVLRELTKI
510 520 530 540 550
NPTTVMSSIY GKAVAAKRLG DVISVSQCVP VNQATVTLRK SMRVPGSETM
560 570 580 590 600
CYSRPLVSFS FINDTKTYEG QLGTDNEIFL TKKMTEVCQA TSQYYFQSGN
610 620 630 640 650
EIHVYNDYHH FKTIELDGIA TLQTFISLNT SLIENIDFAS LELYSRDEQR
660 670 680 690 700
ASNVFDLEGI FREYNFQAQN IAGLRKDLDN AVSNGRNQFV DGLGELMDSL
710 720 730 740 750
GSVGQSITNL VSTVGGLFSS LVSGFISFFK NPFGGMLILV LVAGVVILVI
760 770 780 790 800
SLTRRTRQMS QQPVQMLYPG IDELAQQHAS GEGPGINPIS KTELQAIMLA
810 820 830 840 850
LHEQNQEQKR AAQRAAGPSV ASRALQAARD RFPGLRRRRY HDPETAAALL

GEAETEF
Length:857
Mass (Da):95,609
Last modified:September 22, 2009 - v1
Checksum:i928490D0B9EB8488
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41155.1.

Similar proteinsi

Entry informationi

Entry nameiGB_EBVG
AccessioniPrimary (citable) accession number: P0C762
Secondary accession number(s): Q3KSP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: October 25, 2017
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families