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P0C760

- L_PUUMS

UniProt

P0C760 - L_PUUMS

Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Puumala virus (strain Sotkamo/V-2969/81)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cap snatching Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Cap snatching, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L (EC:2.7.7.48)
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Gene namesi
    Name:L
    OrganismiPuumala virus (strain Sotkamo/V-2969/81)
    Taxonomic identifieri39002 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeHantavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]
    ProteomesiUP000008482: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21562156RNA-directed RNA polymerase LPRO_0000379782Add
    BLAST

    Proteomic databases

    PRIDEiP0C760.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini956 – 1142187RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi273 – 2764Poly-Glu
    Compositional biasi1974 – 200431Asp-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the hantavirus RNA polymerase family.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR026382. CapSnatchArena.
    IPR016268. RNA-dir_pol_hantavirus.
    IPR024378. RNA-dir_pol_N_hantavirus.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view]
    PfamiPF04196. Bunya_RdRp. 1 hit.
    PF12426. DUF3674. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000825. L_HantaV. 1 hit.
    TIGRFAMsiTIGR04202. capSnatchArena. 1 hit.
    PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C760-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKYRDIHER VKEAVPGETS AVECLDLLDR LYAVRHDVVD QMIKHDWSDN     50
    KDREQPIGLV LLMAGVPNDV IQSMEKRVIP GSPSGQILRS FFKMTPDNYK 100
    ITGNLIEFIE VTVTADVARG VREKILKYQG GLEFIEQLLQ IEAQKGNCQS 150
    GFKIKFNVVA VRTDGSNIST QWPSRRNEGV VQAMRLIQAD INFVREHLIK 200
    NDERGALEAM FNLKFHVTGP KVRTFDIPNY RPQPLCQPIL ENLVDYCKNW 250
    LGTDHAFAFK EVTGQRVFNV FREEEEIHAS KYGHSRKPRN FLLCQISLQS 300
    PYLPSTIASD QYDTRLACSE ILKNYPETPL QLLARDMAYK YITLDHDDII 350
    NYYNPRVYFK PTQNIKEPGT FKLNLSNMDP KSKALIDVIS KDSKKGVFGE 400
    LIDSVDVASQ VQHNECSKTI EKILSDLEVN LGDVANGLDQ PKKTTGVDDI 450
    LRKFYDNELV KYLISVIRKT TAWHLGHLLR DITESLIAHA GLKRSKYWSA 500
    HGYACGSVLL CILPSKSLEV AGSFIRFFTV FKEGLGLIDT DNLDSKAEID 550
    GVSWCFSKII SLDLNRLLAL NIAFEKSLLA TATWFQYYTE DQGHFPLQHA 600
    LRSVFAFHFL LSVSQKMKLC AIFDNLRYLI PAVTSTYSGF EPLIRKFFER 650
    PFKSALEVYL YGIIKVLLVS LAQNNKIRFY SRVRLLGLTV DQSTIGASGV 700
    YPSLMSRVVY KHYRSLISEA TTCFFLFEKG LHGNLTEEAK IHLETVEWAR 750
    KFREKERELG SYIMEEGYHI QDVLNNQVAV EQQLFCQEVV ELAAQELNTY 800
    LHAKSQVMAS NIMNKHWDKP YFSQTRNISL KGMSGALQED GHLAASVTLI 850
    EAIRFLNHSQ NNPTVLELYE QTKKQRAQAR IVRKYQRTEA DRGFFITTLP 900
    TRVRLEIIED YYDAIAKVVP EEYISYGGER KILNIQQALE KALRWASGES 950
    EIQSSLGHSI KLKRKLMYVS ADATKWSPGD NSAKFRRFTQ SLYDGLRDDK 1000
    LKNCVVDALR NIYETDFFIS RKLHRYIDNM GELSDEVLDF LSFFPNKVSA 1050
    SIKGNWLQGN LNKCSSLFGA AISLLFKRVW AKLYPELECF FEFAHHSDDA 1100
    LFIYGYLEPI DDGTEWFQYV TQQIQAGNFH WHAVNQEMWK SMFNLHEHIL 1150
    LMGSIKISPK KTTVSPTNAE FLSTFFEGCA VSIPFIKILL GSLSDLPGLG 1200
    YFDDLAAAQS RCVKALDMGA CPQLAQLGIV LCTSKVERLY GTAPGMVNNP 1250
    TAYLKVDRNL IPIPLGGDGS MSIMELATAG IGMADKNILK NAFITYKHAK 1300
    KDNDRYVLGL FKFLMSLSDD IFQHDRLGEF SFVGKVQWKV FTPKSEFEFY 1350
    DQYSRKYLEL WSEQHPVYDY IIPRGRDNLL VYLVRKLNDP SIVTAMTMQS 1400
    PLQLRFRMQA KQHMKVCKLG GEWVTFREVL AAADAFASEY RPTLQDMELF 1450
    QTLVNCTFSK EYAWRDFLNE VQCDVLTTRQ IHRPKVARTF TVKERDQTIQ 1500
    NPITAVIGYK YASKVDEISD VLDSALHPDS LSTDLQLMRE GVYRELGLDI 1550
    SQPNVLKKVA PLLYKSGKSR IVIVQGNVEG TAESICSYWL KTMSLVKTIK 1600
    VKPKKEVLKA VSLYGKKEKV GDLTHLAAMR LCIEVWRWCK ANEQDSVTWL 1650
    KYLVFENKTL EQWVDLFCSR GVLPIDPEIQ CLGLLVYDLK GQKGLLQIQA 1700
    NRRAYSGKQY DAYCVQTYNE ETKLYEGDLR VTFNFGIDCA RLEIFWDKKE 1750
    YILETSITQR NVLKILMEEV TKELLRCGMR FKTEQVNSSR SVVLFKTESG 1800
    FEWGKPNVPC IVYRNCTLRT GLRVRHPTNK AFSITIQANG FRAMAQLDEE 1850
    NPRFLLAHAY HNLKDVRYQA LQAVGNVWFK MTQHKLFINP IISAGLLENF 1900
    MKGLPAAIPP AAYSLIMNKA KISVDLFMFN ELLALINPQN VLNLDGIEET 1950
    SEGFTTVSTI SSTQWSEEVS LTLDDSDDDD DASNLDYTID LDDIDFETID 2000
    LKEDIEHFLQ DESAYTGDLL IQTEETEVRK LRGMIKILEP VKLIKSWVSK 2050
    GLSIDKIYNP VNIILMTRYM SKHYNFQAKQ LSLMDPYDLT EFESVVKGWG 2100
    ECVKDRFIEL DQEAQRKVTE ERVLPEDVLP DSFFSFRHAD ILLKRLFPRD 2150
    SASSFY 2156
    Length:2,156
    Mass (Da):246,999
    Last modified:July 28, 2009 - v1
    Checksum:i3DA19810F1A067F8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti157 – 1571N → D in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti1477 – 14771T → I in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti1776 – 17761R → H in strain: isolate Sotkamo 2009/WHO Arbovirus collection.
    Natural varianti2133 – 21331F → L in strain: isolate Sotkamo 2009/WHO Arbovirus collection.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z66548 Genomic RNA. No translation available.
    HE801635 Genomic RNA. Translation: CCH22849.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z66548 Genomic RNA. No translation available.
    HE801635 Genomic RNA. Translation: CCH22849.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0C760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR026382. CapSnatchArena.
    IPR016268. RNA-dir_pol_hantavirus.
    IPR024378. RNA-dir_pol_N_hantavirus.
    IPR007099. RNA-dir_pol_NSvirus.
    IPR007322. RNA_pol_bunyavir.
    [Graphical view ]
    Pfami PF04196. Bunya_RdRp. 1 hit.
    PF12426. DUF3674. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000825. L_HantaV. 1 hit.
    TIGRFAMsi TIGR04202. capSnatchArena. 1 hit.
    PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the Puumala hantavirus Sotkamo strain L segment."
      Piiparinen H., Vapalahti O., Plyusnin A., Vaheri A., Lankinen H.
      Virus Res. 51:1-7(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Resequencing of the Puumala virus strain Sotkamo from the WHO Arbovirus collection."
      Kurolt I.C., Paessler S., Markotic A.
      Virus Genes 45:389-392(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Sotkamo 2009/WHO Arbovirus collection.

    Entry informationi

    Entry nameiL_PUUMS
    AccessioniPrimary (citable) accession number: P0C760
    Secondary accession number(s): I4EPA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3