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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

BORF2

Organism
Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathway:iDNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711SubstrateBy similarity
Sitei187 – 1871Important for hydrogen atom transferBy similarity
Binding sitei217 – 2171Substrate; via amide nitrogenBy similarity
Active sitei387 – 3871Proton acceptorBy similarity
Active sitei389 – 3891Cysteine radical intermediateBy similarity
Active sitei391 – 3911Proton acceptorBy similarity
Sitei403 – 4031Important for hydrogen atom transferBy similarity
Sitei725 – 7251Important for electron transferBy similarity
Sitei726 – 7261Important for electron transferBy similarity
Sitei822 – 8221Interacts with thioredoxin/glutaredoxinBy similarity
Sitei825 – 8251Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase 140 kDa subunit
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:BORF2
OrganismiEpstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri82830 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007639 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 826826Ribonucleoside-diphosphate reductase large subunitPRO_0000375965Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi187 ↔ 403Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit BaRF1 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1872Substrate bindingBy similarity
Regioni387 – 3915Substrate bindingBy similarity
Regioni594 – 5985Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

KOiK10807.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTSHVEHE LLSKLIDELK VKANSDPEAD VLAGRLLHRL KAESVTHTVA
60 70 80 90 100
EYLEVFSDKF YDEEFFQMHR DELETRVSAF AQSPAYERIV SSGYLSALRY
110 120 130 140 150
YDTYLYVGRS GKQESVQHFY MRLAGFCAST TCLYAGLRAA LQRARPEIES
160 170 180 190 200
DMEVFDYYFE HLTSQTVCCS TPFMRFAGVE NSTLASCILT TPDLSSEWDV
210 220 230 240 250
TQALYRHLGR YLFQRAGVGV GVTGAGQDGK HISLLMRMIN SHVEYHNYGC
260 270 280 290 300
KRPVSVAAYM EPWHSQIFKF LETKLPENHE RCPGIFTGLF VPELFFKLFR
310 320 330 340 350
DTPWSDWYLF DPKDAGDLER LYGEEFEREY YRLVTAGKFC GRVSIKSLMF
360 370 380 390 400
SIVNCAVKAG SPFILLKEAC NAHFWRDLQG EAMNAANLCA EVLQPSRKSV
410 420 430 440 450
ATCNLANICL PRCLVNAPLA VRAQRADTQG DELLLALPRL SVTLPGEGAV
460 470 480 490 500
GDGFSLARLR DATQCATFVV ACSILQGSPT YDSRDMASMG LGVQGLADVF
510 520 530 540 550
ADLGWQYTDP PSRSLNKEIF EHMYFTALCT SSLIGLHTRK IFPGFKQSKY
560 570 580 590 600
AGGWFHWHDW AGTDLSIPRE IWSRLSERIV RDGLFNSQFI ALMPTSGCAQ
610 620 630 640 650
VTGCSDAFYP FYANASTKVT NKEEALRPNR SFWRHVRLDD REALNLVGGR
660 670 680 690 700
VSCLPEALRQ RYLRFQTAFD YNQEDLIQMS RDRAPFVDQS QSHSLFLREE
710 720 730 740 750
DAARASTLAN LLVRSYELGL KTIMYYCRIE KAADLGVMEC KASAALSVPR
760 770 780 790 800
EEQNERSPAE QMPPRPMEPA QVAGPVDIMS KGPGEGPGGW CVPGGLEVCY
810 820
KYRQLFSEDD LLETDGFTER ACESCQ
Length:826
Mass (Da):93,031
Last modified:May 26, 2009 - v1
Checksum:i6E5C25623D371EF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ279927 Genomic DNA. Translation: ABB89232.1.
RefSeqiYP_001129452.1. NC_009334.1.
YP_401655.1. NC_007605.1.

Genome annotation databases

GeneIDi3783725.
5176186.
KEGGivg:3783725.
vg:5176186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ279927 Genomic DNA. Translation: ABB89232.1.
RefSeqiYP_001129452.1. NC_009334.1.
YP_401655.1. NC_007605.1.

3D structure databases

ProteinModelPortaliP0C702.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783725.
5176186.
KEGGivg:3783725.
vg:5176186.

Phylogenomic databases

KOiK10807.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Epstein-Barr virus type 2 strain AG876."
    Dolan A., Addison C., Gatherer D., Davison A.J., McGeoch D.J.
    Virology 350:164-170(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_EBVA8
AccessioniPrimary (citable) accession number: P0C702
Secondary accession number(s): Q777G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: April 1, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.