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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171SubstrateUniRule annotation1
Sitei187Important for hydrogen atom transferUniRule annotation1
Binding sitei217Substrate; via amide nitrogenUniRule annotation1
Active sitei387Proton acceptorUniRule annotation1
Active sitei389Cysteine radical intermediateUniRule annotation1
Active sitei391Proton acceptorUniRule annotation1
Sitei403Important for hydrogen atom transferUniRule annotation1
Sitei725Important for electron transferUniRule annotation1
Sitei726Important for electron transferUniRule annotation1
Sitei822Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei825Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
ORF Names:BORF2
OrganismiEpstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri82830 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007639 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003759651 – 826Ribonucleoside-diphosphate reductase large subunitAdd BLAST826

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi187 ↔ 403Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP0C702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni186 – 187Substrate bindingUniRule annotation2
Regioni387 – 391Substrate bindingUniRule annotation5
Regioni594 – 598Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK10807.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTSHVEHE LLSKLIDELK VKANSDPEAD VLAGRLLHRL KAESVTHTVA
60 70 80 90 100
EYLEVFSDKF YDEEFFQMHR DELETRVSAF AQSPAYERIV SSGYLSALRY
110 120 130 140 150
YDTYLYVGRS GKQESVQHFY MRLAGFCAST TCLYAGLRAA LQRARPEIES
160 170 180 190 200
DMEVFDYYFE HLTSQTVCCS TPFMRFAGVE NSTLASCILT TPDLSSEWDV
210 220 230 240 250
TQALYRHLGR YLFQRAGVGV GVTGAGQDGK HISLLMRMIN SHVEYHNYGC
260 270 280 290 300
KRPVSVAAYM EPWHSQIFKF LETKLPENHE RCPGIFTGLF VPELFFKLFR
310 320 330 340 350
DTPWSDWYLF DPKDAGDLER LYGEEFEREY YRLVTAGKFC GRVSIKSLMF
360 370 380 390 400
SIVNCAVKAG SPFILLKEAC NAHFWRDLQG EAMNAANLCA EVLQPSRKSV
410 420 430 440 450
ATCNLANICL PRCLVNAPLA VRAQRADTQG DELLLALPRL SVTLPGEGAV
460 470 480 490 500
GDGFSLARLR DATQCATFVV ACSILQGSPT YDSRDMASMG LGVQGLADVF
510 520 530 540 550
ADLGWQYTDP PSRSLNKEIF EHMYFTALCT SSLIGLHTRK IFPGFKQSKY
560 570 580 590 600
AGGWFHWHDW AGTDLSIPRE IWSRLSERIV RDGLFNSQFI ALMPTSGCAQ
610 620 630 640 650
VTGCSDAFYP FYANASTKVT NKEEALRPNR SFWRHVRLDD REALNLVGGR
660 670 680 690 700
VSCLPEALRQ RYLRFQTAFD YNQEDLIQMS RDRAPFVDQS QSHSLFLREE
710 720 730 740 750
DAARASTLAN LLVRSYELGL KTIMYYCRIE KAADLGVMEC KASAALSVPR
760 770 780 790 800
EEQNERSPAE QMPPRPMEPA QVAGPVDIMS KGPGEGPGGW CVPGGLEVCY
810 820
KYRQLFSEDD LLETDGFTER ACESCQ
Length:826
Mass (Da):93,031
Last modified:May 26, 2009 - v1
Checksum:i6E5C25623D371EF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ279927 Genomic DNA. Translation: ABB89232.1.
RefSeqiYP_001129452.1. NC_009334.1.
YP_401655.1. NC_007605.1.

Genome annotation databases

GeneIDi3783725.
5176186.
KEGGivg:3783725.
vg:5176186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ279927 Genomic DNA. Translation: ABB89232.1.
RefSeqiYP_001129452.1. NC_009334.1.
YP_401655.1. NC_007605.1.

3D structure databases

ProteinModelPortaliP0C702.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783725.
5176186.
KEGGivg:3783725.
vg:5176186.

Phylogenomic databases

KOiK10807.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_EBVA8
AccessioniPrimary (citable) accession number: P0C702
Secondary accession number(s): Q777G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: June 8, 2016
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.