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P0C6Y4 (R1AB_PEDV7) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p9
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p87
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.-
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PLP1/PLP2
    Papain-like proteinases 1/2
    p195
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p34
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p5
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p23
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p14
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
    p100
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
    p66
    p66-HEL
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismPorcine epidemic diarrhea virus (strain CV777) (PEDV) [Complete proteome]
Taxonomic identifier229032 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length6781 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Sequence caution

The sequence AAK38661.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host IRF3 activity

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6Y4-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6V6-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6V6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 110110Non-structural protein 1 By similarity
PRO_0000037369
Chain111 – 895785Non-structural protein 2 By similarity
PRO_0000037370
Chain896 – 25161621Non-structural protein 3 By similarity
PRO_0000037371
Chain2517 – 2997481Non-structural protein 4 By similarity
PRO_0000037372
Chain2998 – 32993023C-like proteinase By similarity
PRO_0000037373
Chain3300 – 3579280Non-structural protein 6 By similarity
PRO_0000037374
Chain3580 – 366283Non-structural protein 7 By similarity
PRO_0000037375
Chain3663 – 3857195Non-structural protein 8 By similarity
PRO_0000037376
Chain3858 – 3965108Non-structural protein 9 By similarity
PRO_0000037377
Chain3966 – 4100135Non-structural protein 10 By similarity
PRO_0000037378
Chain4101 – 5027927RNA-directed RNA polymerase By similarity
PRO_0000037379
Chain5028 – 5546519Helicase
PRO_0000037380
Chain5547 – 6141595Exoribonuclease By similarity
PRO_0000037381
Chain6142 – 6480339Uridylate-specific endoribonuclease By similarity
PRO_0000037382
Chain6481 – 6781301Putative 2'-O-methyl transferase By similarity
PRO_0000037383

Regions

Transmembrane1959 – 197921Helical; Potential
Transmembrane2022 – 204221Helical; Potential
Transmembrane2105 – 212521Helical; Potential
Transmembrane2127 – 214721Helical; Potential
Transmembrane2150 – 217021Helical; Potential
Transmembrane2528 – 254821Helical; Potential
Transmembrane2619 – 263921Helical; Potential
Transmembrane2654 – 267421Helical; Potential
Transmembrane2754 – 277421Helical; Potential
Transmembrane2787 – 280721Helical; Potential
Transmembrane2814 – 283421Helical; Potential
Transmembrane2863 – 288321Helical; Potential
Transmembrane3336 – 335621Helical; Potential
Transmembrane3361 – 338121Helical; Potential
Transmembrane3399 – 341921Helical; Potential
Transmembrane3431 – 345121Helical; Potential
Transmembrane3454 – 347421Helical; Potential
Transmembrane3476 – 349621Helical; Potential
Transmembrane3500 – 352021Helical; Potential
Domain1057 – 1296240Peptidase C16 1
Domain1297 – 1465169Macro
Domain1691 – 1951261Peptidase C16 2
Domain2998 – 3299302Peptidase C30
Domain4707 – 4869163RdRp catalytic
Domain5028 – 511184CV MBD
Domain5275 – 5466192(+)RNA virus helicase ATP-binding
Domain5467 – 5636170(+)RNA virus helicase C-terminal
Zinc finger1162 – 119332C4-type
Zinc finger4039 – 405517 By similarity
Zinc finger4081 – 409414 By similarity
Nucleotide binding5310 – 53178ATP By similarity
Region1959 – 2170212HD1
Region2528 – 2883356HD2
Region3336 – 3520185HD3
Compositional bias1816 – 1932117Val-rich

Sites

Active site10911For PL1-PRO activity By similarity
Active site12391For PL1-PRO activity By similarity
Active site17291For PL2-PRO activity By similarity
Active site18881For PL2-PRO activity By similarity
Active site30381For 3CL-PRO activity By similarity
Active site31411For 3CL-PRO activity By similarity
Site110 – 1112Cleavage; by PL1-PRO By similarity
Site895 – 8962Cleavage; by PL1-PRO By similarity
Site2516 – 25172Cleavage; by PL2-PRO By similarity
Site2997 – 29982Cleavage; by 3CL-PRO By similarity
Site3299 – 33002Cleavage; by 3CL-PRO By similarity
Site3579 – 35802Cleavage; by 3CL-PRO By similarity
Site3662 – 36632Cleavage; by 3CL-PRO By similarity
Site3857 – 38582Cleavage; by 3CL-PRO By similarity
Site3965 – 39662Cleavage; by 3CL-PRO By similarity
Site4100 – 41012Cleavage; by 3CL-PRO By similarity
Site5027 – 50282Cleavage; by 3CL-PRO By similarity
Site5546 – 55472Cleavage; by 3CL-PRO By similarity
Site6141 – 61422Cleavage; by 3CL-PRO By similarity
Site6480 – 64812Cleavage; by 3CL-PRO By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: C5E29CBA752AD5BB

FASTA6,781753,158
        10         20         30         40         50         60 
MASNHVTLAF ANDAEISAFG FCTASEAVSY YSEAAASGFM QCRFVSLDLA DTVEGLLPED 

        70         80         90        100        110        120 
YVMVVIGTTK LSAYVDTFGS RPRNICGWLL FSNCNYFLEE LELTFGRRGG NIVPVDQYMC 

       130        140        150        160        170        180 
GADGKPVLQE SEWEYTDFFA DSEDGQLNIA GITYVKAWIV ERSDVSYASQ NLTSIKSITY 

       190        200        210        220        230        240 
CSTYEHTFLD GTAMKVARTP KIKKNVVLSE PLATIYREIG SPFVDNGSDA RSIIRRPVFL 

       250        260        270        280        290        300 
HAFVKCKCGS YHWTVGDWTS YVSTCCGFKC KPVLVASCSA MPGSVVVTRA GAGTGVKYYN 

       310        320        330        340        350        360 
NMFLRHVADI DGLAFWRILK VQSKDDLACS GKFLEHHEEG FTDPCYFLND SSLATKLKFD 

       370        380        390        400        410        420 
ILSGKFSDEV KQAIIAGHVV VGSALVDIVD DALGQPWFIR KLGDLASAPW EQLKAVVRGL 

       430        440        450        460        470        480 
GLLSDEVVLF GKRLSCATLS IVNGVFEFLA DVPEKLAAAV TVFVNFLNEF FESACDCLKV 

       490        500        510        520        530        540 
GGKTFNKVGS YVLFDNALVK LVKAKARGPR QAGICEVRYT SLVVGSTTKV VSKRVENANV 

       550        560        570        580        590        600 
NLVVVDEDVT LNTTGRTVVV DGLAFFESDG FYRHLADADV VIEHPVYKSA CELKPVFECD 

       610        620        630        640        650        660 
PIPDFPLPVA ASVAELCVQT DLLLKNYNTP YKTYSCVVRG DKCCITCTLQ FKAPSYVEDA 

       670        680        690        700        710        720 
VNFVDLCTKN IGTAGFHEFY ITAHEQQDLQ GFLTTCCTMS GFECFMPTIP QCPAVLEEID 

       730        740        750        760        770        780 
GGSIWRSFIT GLNTMWDFCK RLKVSFGLDG IVVTVARKFK RLGALLAEMY NTYLSTVVEN 

       790        800        810        820        830        840 
LVLAGVSFKY YATSVPKIVL GGCFHSVKSV FASVFQIPVQ AGIEKFKVFL NCVHPVVPRV 

       850        860        870        880        890        900 
IETSFVELEE TTFKPPALNG GIAIVDGFAF YYDGTLYYPT DGNSVVPICF KKKGGGDVKF 

       910        920        930        940        950        960 
SDEVSVKTID PVYKVSLEFE FESETIMAVL NKAVGNRIKV TGGWDDVVEY INVAIEVLKD 

       970        980        990       1000       1010       1020 
HVEVPKYYIY DEEGGTDPNL PVMVSQWPLN DDTISQDLLD VEVVTDAPID SEGDEVDSSA 

      1030       1040       1050       1060       1070       1080 
PEKVADVANS EPGDDGLPVA PETNVESEVE EVAATLSFIK DTPSTVTKDP FAFDFVSYGG 

      1090       1100       1110       1120       1130       1140 
LKVLRQSHNN CWVTSTLVQL QLLGIVDDPA MELFSAGRVG PMVRKCYESQ KAILGSLGDV 

      1150       1160       1170       1180       1190       1200 
SACLESLTKD LHTLKITCSV VCGCGTGERI YEGCAFRMTP TLEPFPYGAC AQCAQVLMHT 

      1210       1220       1230       1240       1250       1260 
FKSIVGTGIF CRDTTALSLD SLVVKPLCAA AFIGKDSGHY VTNFYDAAMA IDGYGRHQIK 

      1270       1280       1290       1300       1310       1320 
YDTLNTICVK DVNWTAPLVP AVDSVVEPVV KPFYSYKNVD FYQGDFSDLV KLPCDFVVNA 

      1330       1340       1350       1360       1370       1380 
ANEKLSHGGG IAKAIDVYTK GMLQKCSNDY IKAHGPIKVG RGVMLEALGL KVFNVVGPRK 

      1390       1400       1410       1420       1430       1440 
GKHAPELLVK AYKSVFANSG VALTPLISVG IFSVPLEESL SAFLACVGDR HCKCFCYGDK 

      1450       1460       1470       1480       1490       1500 
EREAIIKYMD GLVDAIFKEA LVDTTPVQED VQQVSQKPVL PNFEPFRIEG AHAFYECNPE 

      1510       1520       1530       1540       1550       1560 
GLMSLGADKL VLFTNSNLDF CSVGKCLNDV TSGALLEAIN VFKKSNKTVP AGNCVTLDCA 

      1570       1580       1590       1600       1610       1620 
NMISITMVVL PFDGDANYDK NYARAVVKVS KLKGKLVLAV DDATLYSKLS HLSVLGFVST 

      1630       1640       1650       1660       1670       1680 
PDDVERFYAN KSVVIKVTED TRSVKAVKVE STATYGQQIG PCLVNDTVVT DNKPVVADVV 

      1690       1700       1710       1720       1730       1740 
AKVVPNANWD SHYGFDKAGE FHMLDHTGFT FPSEVVNGRR VIKTTDNNCW VNVTCLQLQF 

      1750       1760       1770       1780       1790       1800 
ARFRFKSAGL QAMWESYCTG DVAMFVHWLY WLTGVDKGQP SDSENALNML SKYIVPAGSV 

      1810       1820       1830       1840       1850       1860 
TIERVTHDGC CCSKRVVTAP VVNASVLKLG VEDGLCPHGL NYIGKVVVVK GTTIVVNVGK 

      1870       1880       1890       1900       1910       1920 
PVVAPSHLFL KGVSYTTFLD NGNGVVGHYT VFDHGTGMVH DGDAFVPGDL NVSPVTNVVV 

      1930       1940       1950       1960       1970       1980 
SEQTAVVIKD PVKKAELDAT KLLDTMNYAS ERFFSFGDFM SRNLITVFLY ILSILGLCFR 

      1990       2000       2010       2020       2030       2040 
AFRKRDVKVL AGVPQRTGII LRKSMRYNAK ALGVFFKLKL YWFKVLGKFS LGIYALYALL 

      2050       2060       2070       2080       2090       2100 
FMTIRFTPIG SPVCDDVVAG YANSSFDKNE YCNSVICKVC LYGYQELSDF SHTQVVWQHL 

      2110       2120       2130       2140       2150       2160 
RDPLIGNVMP FFYLAFLAIF GGVYVKAITL YFIFQYLNSL GVFLGLQQSI WFLQLVPFDV 

      2170       2180       2190       2200       2210       2220 
FGDEIVVFFI VTRVLMFIKH VCLGCDKASC VACSKSARLK RVPVQTIFQG TSKSFYVHAN 

      2230       2240       2250       2260       2270       2280 
GGSKFCKKHN FFCLNCDSYG PGCTFINDVI ATEVGNVVKL NVQPTGPATI LIDKVEFSNG 

      2290       2300       2310       2320       2330       2340 
FYYLYSGDTF WKYNFDITDS KYTCKEALKN CSIITDFIVF NNNGSNVNQV KNACVYFSQM 

      2350       2360       2370       2380       2390       2400 
LCKPVKLVDS ALLASLSVDF GASLHSAFVS VLSNSFGKDL SSCNDMQDCK STLGFDDVPL 

      2410       2420       2430       2440       2450       2460 
DTFNAAVAEA HRYDVLLTDM SFNNFTTSYA KPEEKFPVHD IATCMRVGAK IVNHNVLVKD 

      2470       2480       2490       2500       2510       2520 
SIPVVWLVRD FIALSEETRK YIIRTTKVKG ITFMLTFNDC RMHTTIPTVC IANKKGAGLP 

      2530       2540       2550       2560       2570       2580 
SFSKVKKFFW FLCLFIVAAF FALSFLDFST QVSSDSDYDF KYIESGQLKT FDNPLSCVHN 

      2590       2600       2610       2620       2630       2640 
VFINFDQWHD AKFGFTPVNN PSCPIVVGVS DEARTVPGIP AGVYLAGKTL VFAINTIFGT 

      2650       2660       2670       2680       2690       2700 
SGLCFDASGV ADKGACIFNS ACTTLSGLGG TAVYCYKNGL VEGAKLYSEL APHSYYKMVD 

      2710       2720       2730       2740       2750       2760 
GNAVSLPEII SRGFGIRTIR TKAMTYCRVG QCVQSAEGVC FGADRFFVYN AESGSDFVCG 

      2770       2780       2790       2800       2810       2820 
TGLFTLLMNV ISVFSKTVPV TVLSGQILFN CIIAFVAVAV CFLFTKFKRM FGDMSVGVFT 

      2830       2840       2850       2860       2870       2880 
VGACTLLNNV SYIVTQNTLG MLGYATLYFL CTKGVRYMWI WHLGFLISYI LIAPWWVLMV 

      2890       2900       2910       2920       2930       2940 
YAFSAIFEFM PNLFKLKVST QLFEGDKFVG SFENAAAGTF VLDMHAYERL ANSISTEKLR 

      2950       2960       2970       2980       2990       3000 
QYASTYNKYK YYSGSASEAD YRLACFAHLA KAMMDYASNH NDTLYTPPTV SYNSTLQAGL 

      3010       3020       3030       3040       3050       3060 
RKMAQPSGVV EKCIVRVCYG NMALNGLWLG DIVMCPRHVI ASSTTSTIDY DYALSVLRLH 

      3070       3080       3090       3100       3110       3120 
NFSISSGNVF LGVVSATMRG ALLQIKVNQN NVHTPKYTYR TVRPGESFNI LACYDGAAAG 

      3130       3140       3150       3160       3170       3180 
VYGVNMRSNY TIRGSFINGA CGSPGYNINN GTVEFCYLHQ LELGSGCHVG SDLDGVMYGG 

      3190       3200       3210       3220       3230       3240 
YEDQPTLQVE GASSLFTENV LAFLYAALIN GSTWWLSSSR IAVDRFNEWA VHNGMTTVGN 

      3250       3260       3270       3280       3290       3300 
TDCFSILAAK TGVDVQRLLA SIQSLHKNFG GKQILGHTSL TDEFTTGEVV RQMYGVNLQG 

      3310       3320       3330       3340       3350       3360 
GYVSRACRNV LLVGSFLTFF WSELVSYTKF FWVNPGYVTP MFACLSLLSS LLMFTLKHKT 

      3370       3380       3390       3400       3410       3420 
LFFQVFLIPA LIVTSCINLA FDVEVYNYLA EHFDYHVSLM GFNAQGLVNI FVCFVVTILH 

      3430       3440       3450       3460       3470       3480 
GTYTWRFFNT PASSVTYVVA LLTAAYNYFY ASDILSCAMT LFASVTGNWF VGAVCYKVAV 

      3490       3500       3510       3520       3530       3540 
YMALRFPTFV AIFGDIKSVM FCYLVLGYFT CCFYGILYWF NRFFKVSVGV YDYTVSAAEF 

      3550       3560       3570       3580       3590       3600 
KYMVANGLRA PTGTLDSLLL SAKLIGIGGE RNIKISSVQS KLTDIKCSNV VLLGCLSSMN 

      3610       3620       3630       3640       3650       3660 
VSANSTEWAY CVDLHNKINL CNDPEKAQEM LLALLAFFLS KNSAFGLDDL LESYFNDNSM 

      3670       3680       3690       3700       3710       3720 
LQSVASTYVG LPSYVIYENA RQQYEDAVNN GSPPQLVKQL RHAMNVAKSE FDREASTQRK 

      3730       3740       3750       3760       3770       3780 
LDRMAEQAAA QMYKEARAVN RKSKVVSAMH SLLFGMLRRL DMSSVDTILN LAKDGVVPLS 

      3790       3800       3810       3820       3830       3840 
VIPAVSATKL NIVTSDIDSY NRIQREGCVH YAGTIWNIID IKDNDGKVVH VKEVTAQNAE 

      3850       3860       3870       3880       3890       3900 
SLSWPLVLGC ERIVKLQNNE IIPGKLKQRS IKAEGDGIVG EGKALYNNEG GRTFMYAFIS 

      3910       3920       3930       3940       3950       3960 
DKPDLRVVKW EFDGGCNTIE LEPPRKFLVD SPNGAQIKYL YFVRNLNTLR RGAVLGYIGA 

      3970       3980       3990       4000       4010       4020 
TVRLQAGKQT EQAINSSLLT LCAFAVDPAK TYIDAVKSGH KPVGNCVKML ANGSGNGQAV 

      4030       4040       4050       4060       4070       4080 
TNGVEASTNQ DSYGGASVCL YCRAHVEHPS MDGFCRLKGK YVQVPLGTVD PIRFVLENDV 

      4090       4100       4110       4120       4130       4140 
CKVCGCWLSN GCTCDRSIMQ STDMAYLNRV RGSSAARLEP CNGTDTQHVY RAFDIYNKDV 

      4150       4160       4170       4180       4190       4200 
ACLGKFLKVN CVRLKNLDKH DAFYVVKRCT KSAMEHEQSI YSRLEKCGAI AEHDFFTWKD 

      4210       4220       4230       4240       4250       4260 
GRAIYGNVCR KDLTEYTMMD LCYALRNFDE NNCDVLKSIL IKVGACEESY FNNKVWFDPV 

      4270       4280       4290       4300       4310       4320 
ENEDIHRVYA LLGTIVARAM LKCVKFCDAM VEQGIVGVVT LDNQDLNGDF YDFGDFTCSI 

      4330       4340       4350       4360       4370       4380 
KGMGVPICTS YYSYMMPVMG MTNCLASECF VKSDIFGEDF KSYDLLEYDF TEHKTALFNK 

      4390       4400       4410       4420       4430       4440 
YFKYWGLQYH PNCVDCSDEQ CIVHCANFNT LFSTTIPITA FGPLCRKCWI DGVPLVTTAG 

      4450       4460       4470       4480       4490       4500 
YHFKQLGIVW NNDLNLHSSR LSINELLQFC SDPALLIASS PALVDQRTVC FSVAALGTGM 

      4510       4520       4530       4540       4550       4560 
TNQTVKPGHF NKEFYDFLLE QGFFSEGSEL TLKHFFFAQK VDAAVKDFDY YRYNRPTVLD 

      4570       4580       4590       4600       4610       4620 
ICQARVVYQI VQRYFDIYEG GCITAKEVVV TNLNKSAGYP LNKFGKAGLY YESLSYEEQD 

      4630       4640       4650       4660       4670       4680 
ELYAYTKRNI LPTMTQLNLK YAISGKERAR TVGGVSLLST MTTRQYHQKH LKSIVNTRGA 

      4690       4700       4710       4720       4730       4740 
SVVIGTTKFY GGWDNMLKNL IDGVENPCLM GWDYPKCDRA LPNMIRMISA MILGSKHTTC 

      4750       4760       4770       4780       4790       4800 
CSSTDRFFRL CNELAQVLTE VVYSNGGFYL KPGGTTSGDA TTAYANSVFN IFQAVSANVN 

      4810       4820       4830       4840       4850       4860 
KLLSVDSNVC HNLEVKQLQR KLYECCYRST IVDDQFVVEY YGYLRKHFSM MILSDDGVVC 

      4870       4880       4890       4900       4910       4920 
YNNDYASLGY VADLNAFKAV LYYQNNVFMS ASKCWIEPDI NKGPHEFCSQ HTMQIVDKEG 

      4930       4940       4950       4960       4970       4980 
TYYLPYPDPS RILSAGVFVD DVVKTDAVVL LERYVSLAID AYPLSKHENP EYKKVFYVLL 

      4990       5000       5010       5020       5030       5040 
DWVKHLYKTL NAGVLESFSV TLLEDSTAKF WDESFYANMY EKSAVLQSAG LCVVCGSQTV 

      5050       5060       5070       5080       5090       5100 
LRCGDCLRRP MLCTKCAYDH VIGTTHKFIL AITPYVCCAS DCGVNDVTKL YLGGLSYWCH 

      5110       5120       5130       5140       5150       5160 
EHKPRLAFPL CSAGNVFGLY KNSATGSPDV EDFNRIATSD WTDVSDYRLA NDVKDSLRLF 

      5170       5180       5190       5200       5210       5220 
AAETIKAKEE SVKSSYACAT LHEVVGPKEL LLKWEVGRPK PPLNRNSVFT CYHITKNTKF 

      5230       5240       5250       5260       5270       5280 
QIGEFVFEKA EYDNDAVTYK TTATTKLVPG MVFVLTSHNV QPLRAPTIAN QERYSTIHKL 

      5290       5300       5310       5320       5330       5340 
HPAFNIPEAY SSLVPYYQLI GKQKITTIQG PPGSGKSHCV IGLGLYYPGA RIVFTACSHA 

      5350       5360       5370       5380       5390       5400 
AVDSLCVKAS TAYSNDKCSR IIPQRARVEC YDGFKSNNTS AQYLFSTVNA LPECNADIVV 

      5410       5420       5430       5440       5450       5460 
VDEVSMCTNY DLSVINQRIS YRHVVYVGDP QQLPAPRVMI SRGTLEPKDY NVVTQRMCAL 

      5470       5480       5490       5500       5510       5520 
KPDVFLHKCY RCPAEIVRTV SEMVYENQFI PVHPDSKQCF KIFCKGNVQV DNGSSINRRQ 

      5530       5540       5550       5560       5570       5580 
LDVVRMFLAK NPRWSKAVFI SPYNSQNYVA SRLLGLQIQT VDSSQGSEYD YVIYAQTSDT 

      5590       5600       5610       5620       5630       5640 
AHASNVNRFN VAITRAKKGI LCIMCDRSLF DLLKFFELKL SDLQANEGCG LFKDCSRGDD 

      5650       5660       5670       5680       5690       5700 
LLPPSHANTF MSLADNFKTD QYLAVQIGVN GPIKYEHVIS FMGFRFDINI PNHHTLFCTR 

      5710       5720       5730       5740       5750       5760 
DFAMRNVRGW LGFDVEGAHV VGSNVGTNVP LQLGFSNGVD FVVRPEGCVV TESGDYIKPV 

      5770       5780       5790       5800       5810       5820 
RARAPPGEQF AHLLPLLKRG QPWDVVRKRI VQMCSDYLAN LSDILIFVLW AGGLELTTMR 

      5830       5840       5850       5860       5870       5880 
YFVKIGPSKS CDCGKVATCY NSALHTYCCF KHALGCDYLY NPYCIDIQQW GYKGSLSLNH 

      5890       5900       5910       5920       5930       5940 
HEHCNVHRNE HVASGDAIMT RCLAIHDCFV KNVDWSITYP FIGNEAVINK SGRIVQSHTM 

      5950       5960       5970       5980       5990       6000 
RSVLKLYNPK AIYDIGNPKG IRCAVTDAKW FCFDKNPTNS NVKTLEYDYI THGQFDGLCL 

      6010       6020       6030       6040       6050       6060 
FWNCNVDMYP EFSVVCRFDT RCRSPLNLEG CNGGSLYVNN HAFHTPAFDK RAFAKLKPMP 

      6070       6080       6090       6100       6110       6120 
FFFYDDTECD KLQDSINYVP LRASNCITKC NVGGAVCSKH CAMYHSYVNA YNTFTSAGFT 

      6130       6140       6150       6160       6170       6180 
IWVPTSFDTY NLWQTFSNNL QGLENIAFNV LKKGSFVGDE GELPVAVVND KVLVRDGTVD 

      6190       6200       6210       6220       6230       6240 
TLVFTNKTSL PTNVAFELYA KRKVGLTPPI TILRNLGVVC TSKCVIWDYE AERPLTTFTK 

      6250       6260       6270       6280       6290       6300 
DVCKYTDFEG DVCTLFDNSI VGSLERFSMT QNAVLMSLTA VKKLTGIKLT YGYLNGVPVN 

      6310       6320       6330       6340       6350       6360 
THEDKPFTWY IYTRKNGKFE DYPDGYFTQG RTTADFSPRS DMEKDFLSMD MGLFINKYGL 

      6370       6380       6390       6400       6410       6420 
EDYGFEHVVY GDVSKTTLGG LHLLISQVRL ACMGVLKIDE FVSSNDSTLK SCTVTYADNP 

      6430       6440       6450       6460       6470       6480 
SSKMVCTYMD LLLDDFVSIL KSLDLSVVSK VHEVMVDCKM WRWMLWCKDH KLQTFYPQLQ 

      6490       6500       6510       6520       6530       6540 
ASEWKCGYSM PSIYKIQRMC LEPCNLYNYG AGVKLPDGIM FNVVKYTQLC QYLNSTTMCV 

      6550       6560       6570       6580       6590       6600 
PHHMRVLHLG AGSDKGVAPG TAVLRRWLPL DAIIVDNDSV DYVSDADYSV TGDCSTLYLS 

      6610       6620       6630       6640       6650       6660 
DKFDLVISDM YDGKIKSCDG ENVSKEGFFP YINGVITEKL ALGGTVAIKV TEFSWNKKLY 

      6670       6680       6690       6700       6710       6720 
ELIQKFEYWT MFCTSVNTSS SEAFLIGVHY LGDFASGAVI DGNTMHANYI FWRNSTIMTM 

      6730       6740       6750       6760       6770       6780 
SYNSVLDLSK FNCKHKATVV VNLKDSSISD VVLGLLKNGK LLVRNNDAIC GFSNHLVNVN 


K 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6V6.

References

[1]"Further analysis of the genome of porcine epidemic diarrhea virus."
Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
Kocherhans R., Bridgen A., Ackermann M., Tobler K.
Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF353511 Genomic RNA. Translation: AAK38661.1. Sequence problems.
RefSeqNP_598309.2. NC_003436.1.

3D structure databases

ProteinModelPortalP0C6Y4.
SMRP0C6Y4. Positions 2998-3297, 3860-3964, 3972-4096.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID935181.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR011050. Pectin_lyase_fold/virulence.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF51126. SSF51126. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_PEDV7
AccessionPrimary (citable) accession number: P0C6Y4
Secondary accession number(s): Q91AV2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries