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P0C6Y4

- R1AB_PEDV7

UniProt

P0C6Y4 - R1AB_PEDV7

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Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Porcine epidemic diarrhea virus (strain CV777) (PEDV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).By similarity
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei110 – 1112Cleavage; by PL1-PROBy similarity
Sitei895 – 8962Cleavage; by PL1-PROBy similarity
Active sitei1091 – 10911For PL1-PRO activityPROSITE-ProRule annotation
Active sitei1239 – 12391For PL1-PRO activityPROSITE-ProRule annotation
Active sitei1729 – 17291For PL2-PRO activityPROSITE-ProRule annotation
Active sitei1888 – 18881For PL2-PRO activityPROSITE-ProRule annotation
Sitei2516 – 25172Cleavage; by PL2-PROBy similarity
Sitei2997 – 29982Cleavage; by 3CL-PROBy similarity
Active sitei3038 – 30381For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3141 – 31411For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3299 – 33002Cleavage; by 3CL-PROBy similarity
Sitei3579 – 35802Cleavage; by 3CL-PROBy similarity
Sitei3662 – 36632Cleavage; by 3CL-PROBy similarity
Sitei3857 – 38582Cleavage; by 3CL-PROBy similarity
Sitei3965 – 39662Cleavage; by 3CL-PROBy similarity
Sitei4100 – 41012Cleavage; by 3CL-PROBy similarity
Sitei5027 – 50282Cleavage; by 3CL-PROBy similarity
Sitei5546 – 55472Cleavage; by 3CL-PROBy similarity
Sitei6141 – 61422Cleavage; by 3CL-PROBy similarity
Sitei6480 – 64812Cleavage; by 3CL-PROBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1162 – 119332C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4039 – 405517By similarityAdd
BLAST
Zinc fingeri4081 – 409414By similarityAdd
BLAST
Nucleotide bindingi5310 – 53178ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. methyltransferase activity Source: InterPro
  7. omega peptidase activity Source: InterPro
  8. RNA binding Source: UniProtKB-KW
  9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of host autophagy Source: UniProtKB-KW
  2. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  3. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral genome replication Source: InterPro
  6. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p9
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p87
Alternative name(s):
PL1-PRO/PL2-PRO
PLP1/PLP2
Papain-like proteinases 1/2
p195
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p34
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p5
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p23
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p14
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
p100
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
p66
p66-HEL
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiPorcine epidemic diarrhea virus (strain CV777) (PEDV)
Taxonomic identifieri229032 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008159: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1959 – 197921HelicalSequence AnalysisAdd
BLAST
Transmembranei2022 – 204221HelicalSequence AnalysisAdd
BLAST
Transmembranei2105 – 212521HelicalSequence AnalysisAdd
BLAST
Transmembranei2127 – 214721HelicalSequence AnalysisAdd
BLAST
Transmembranei2150 – 217021HelicalSequence AnalysisAdd
BLAST
Transmembranei2528 – 254821HelicalSequence AnalysisAdd
BLAST
Transmembranei2619 – 263921HelicalSequence AnalysisAdd
BLAST
Transmembranei2654 – 267421HelicalSequence AnalysisAdd
BLAST
Transmembranei2754 – 277421HelicalSequence AnalysisAdd
BLAST
Transmembranei2787 – 280721HelicalSequence AnalysisAdd
BLAST
Transmembranei2814 – 283421HelicalSequence AnalysisAdd
BLAST
Transmembranei2863 – 288321HelicalSequence AnalysisAdd
BLAST
Transmembranei3336 – 335621HelicalSequence AnalysisAdd
BLAST
Transmembranei3361 – 338121HelicalSequence AnalysisAdd
BLAST
Transmembranei3399 – 341921HelicalSequence AnalysisAdd
BLAST
Transmembranei3431 – 345121HelicalSequence AnalysisAdd
BLAST
Transmembranei3454 – 347421HelicalSequence AnalysisAdd
BLAST
Transmembranei3476 – 349621HelicalSequence AnalysisAdd
BLAST
Transmembranei3500 – 352021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 110110Non-structural protein 1By similarityPRO_0000037369Add
BLAST
Chaini111 – 895785Non-structural protein 2By similarityPRO_0000037370Add
BLAST
Chaini896 – 25161621Non-structural protein 3By similarityPRO_0000037371Add
BLAST
Chaini2517 – 2997481Non-structural protein 4By similarityPRO_0000037372Add
BLAST
Chaini2998 – 32993023C-like proteinaseBy similarityPRO_0000037373Add
BLAST
Chaini3300 – 3579280Non-structural protein 6By similarityPRO_0000037374Add
BLAST
Chaini3580 – 366283Non-structural protein 7By similarityPRO_0000037375Add
BLAST
Chaini3663 – 3857195Non-structural protein 8By similarityPRO_0000037376Add
BLAST
Chaini3858 – 3965108Non-structural protein 9By similarityPRO_0000037377Add
BLAST
Chaini3966 – 4100135Non-structural protein 10By similarityPRO_0000037378Add
BLAST
Chaini4101 – 5027927RNA-directed RNA polymeraseBy similarityPRO_0000037379Add
BLAST
Chaini5028 – 5546519HelicasePRO_0000037380Add
BLAST
Chaini5547 – 6141595ExoribonucleaseBy similarityPRO_0000037381Add
BLAST
Chaini6142 – 6480339Uridylate-specific endoribonucleaseBy similarityPRO_0000037382Add
BLAST
Chaini6481 – 6781301Putative 2'-O-methyl transferaseBy similarityPRO_0000037383Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6Y4.
SMRiP0C6Y4. Positions 2998-3297, 3860-3964, 3972-4096.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1057 – 1296240Peptidase C16 1PROSITE-ProRule annotationAdd
BLAST
Domaini1297 – 1465169MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1691 – 1951261Peptidase C16 2PROSITE-ProRule annotationAdd
BLAST
Domaini2998 – 3299302Peptidase C30PROSITE-ProRule annotationAdd
BLAST
Domaini4707 – 4869163RdRp catalyticAdd
BLAST
Domaini5028 – 511184CV MBDAdd
BLAST
Domaini5275 – 5466192(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini5467 – 5636170(+)RNA virus helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1959 – 2170212HD1Add
BLAST
Regioni2528 – 2883356HD2Add
BLAST
Regioni3336 – 3520185HD3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1816 – 1932117Val-richAdd
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1162 – 119332C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4039 – 405517By similarityAdd
BLAST
Zinc fingeri4081 – 409414By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR011050. Pectin_lyase_fold/virulence.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF51126. SSF51126. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6Y4-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MASNHVTLAF ANDAEISAFG FCTASEAVSY YSEAAASGFM QCRFVSLDLA
60 70 80 90 100
DTVEGLLPED YVMVVIGTTK LSAYVDTFGS RPRNICGWLL FSNCNYFLEE
110 120 130 140 150
LELTFGRRGG NIVPVDQYMC GADGKPVLQE SEWEYTDFFA DSEDGQLNIA
160 170 180 190 200
GITYVKAWIV ERSDVSYASQ NLTSIKSITY CSTYEHTFLD GTAMKVARTP
210 220 230 240 250
KIKKNVVLSE PLATIYREIG SPFVDNGSDA RSIIRRPVFL HAFVKCKCGS
260 270 280 290 300
YHWTVGDWTS YVSTCCGFKC KPVLVASCSA MPGSVVVTRA GAGTGVKYYN
310 320 330 340 350
NMFLRHVADI DGLAFWRILK VQSKDDLACS GKFLEHHEEG FTDPCYFLND
360 370 380 390 400
SSLATKLKFD ILSGKFSDEV KQAIIAGHVV VGSALVDIVD DALGQPWFIR
410 420 430 440 450
KLGDLASAPW EQLKAVVRGL GLLSDEVVLF GKRLSCATLS IVNGVFEFLA
460 470 480 490 500
DVPEKLAAAV TVFVNFLNEF FESACDCLKV GGKTFNKVGS YVLFDNALVK
510 520 530 540 550
LVKAKARGPR QAGICEVRYT SLVVGSTTKV VSKRVENANV NLVVVDEDVT
560 570 580 590 600
LNTTGRTVVV DGLAFFESDG FYRHLADADV VIEHPVYKSA CELKPVFECD
610 620 630 640 650
PIPDFPLPVA ASVAELCVQT DLLLKNYNTP YKTYSCVVRG DKCCITCTLQ
660 670 680 690 700
FKAPSYVEDA VNFVDLCTKN IGTAGFHEFY ITAHEQQDLQ GFLTTCCTMS
710 720 730 740 750
GFECFMPTIP QCPAVLEEID GGSIWRSFIT GLNTMWDFCK RLKVSFGLDG
760 770 780 790 800
IVVTVARKFK RLGALLAEMY NTYLSTVVEN LVLAGVSFKY YATSVPKIVL
810 820 830 840 850
GGCFHSVKSV FASVFQIPVQ AGIEKFKVFL NCVHPVVPRV IETSFVELEE
860 870 880 890 900
TTFKPPALNG GIAIVDGFAF YYDGTLYYPT DGNSVVPICF KKKGGGDVKF
910 920 930 940 950
SDEVSVKTID PVYKVSLEFE FESETIMAVL NKAVGNRIKV TGGWDDVVEY
960 970 980 990 1000
INVAIEVLKD HVEVPKYYIY DEEGGTDPNL PVMVSQWPLN DDTISQDLLD
1010 1020 1030 1040 1050
VEVVTDAPID SEGDEVDSSA PEKVADVANS EPGDDGLPVA PETNVESEVE
1060 1070 1080 1090 1100
EVAATLSFIK DTPSTVTKDP FAFDFVSYGG LKVLRQSHNN CWVTSTLVQL
1110 1120 1130 1140 1150
QLLGIVDDPA MELFSAGRVG PMVRKCYESQ KAILGSLGDV SACLESLTKD
1160 1170 1180 1190 1200
LHTLKITCSV VCGCGTGERI YEGCAFRMTP TLEPFPYGAC AQCAQVLMHT
1210 1220 1230 1240 1250
FKSIVGTGIF CRDTTALSLD SLVVKPLCAA AFIGKDSGHY VTNFYDAAMA
1260 1270 1280 1290 1300
IDGYGRHQIK YDTLNTICVK DVNWTAPLVP AVDSVVEPVV KPFYSYKNVD
1310 1320 1330 1340 1350
FYQGDFSDLV KLPCDFVVNA ANEKLSHGGG IAKAIDVYTK GMLQKCSNDY
1360 1370 1380 1390 1400
IKAHGPIKVG RGVMLEALGL KVFNVVGPRK GKHAPELLVK AYKSVFANSG
1410 1420 1430 1440 1450
VALTPLISVG IFSVPLEESL SAFLACVGDR HCKCFCYGDK EREAIIKYMD
1460 1470 1480 1490 1500
GLVDAIFKEA LVDTTPVQED VQQVSQKPVL PNFEPFRIEG AHAFYECNPE
1510 1520 1530 1540 1550
GLMSLGADKL VLFTNSNLDF CSVGKCLNDV TSGALLEAIN VFKKSNKTVP
1560 1570 1580 1590 1600
AGNCVTLDCA NMISITMVVL PFDGDANYDK NYARAVVKVS KLKGKLVLAV
1610 1620 1630 1640 1650
DDATLYSKLS HLSVLGFVST PDDVERFYAN KSVVIKVTED TRSVKAVKVE
1660 1670 1680 1690 1700
STATYGQQIG PCLVNDTVVT DNKPVVADVV AKVVPNANWD SHYGFDKAGE
1710 1720 1730 1740 1750
FHMLDHTGFT FPSEVVNGRR VIKTTDNNCW VNVTCLQLQF ARFRFKSAGL
1760 1770 1780 1790 1800
QAMWESYCTG DVAMFVHWLY WLTGVDKGQP SDSENALNML SKYIVPAGSV
1810 1820 1830 1840 1850
TIERVTHDGC CCSKRVVTAP VVNASVLKLG VEDGLCPHGL NYIGKVVVVK
1860 1870 1880 1890 1900
GTTIVVNVGK PVVAPSHLFL KGVSYTTFLD NGNGVVGHYT VFDHGTGMVH
1910 1920 1930 1940 1950
DGDAFVPGDL NVSPVTNVVV SEQTAVVIKD PVKKAELDAT KLLDTMNYAS
1960 1970 1980 1990 2000
ERFFSFGDFM SRNLITVFLY ILSILGLCFR AFRKRDVKVL AGVPQRTGII
2010 2020 2030 2040 2050
LRKSMRYNAK ALGVFFKLKL YWFKVLGKFS LGIYALYALL FMTIRFTPIG
2060 2070 2080 2090 2100
SPVCDDVVAG YANSSFDKNE YCNSVICKVC LYGYQELSDF SHTQVVWQHL
2110 2120 2130 2140 2150
RDPLIGNVMP FFYLAFLAIF GGVYVKAITL YFIFQYLNSL GVFLGLQQSI
2160 2170 2180 2190 2200
WFLQLVPFDV FGDEIVVFFI VTRVLMFIKH VCLGCDKASC VACSKSARLK
2210 2220 2230 2240 2250
RVPVQTIFQG TSKSFYVHAN GGSKFCKKHN FFCLNCDSYG PGCTFINDVI
2260 2270 2280 2290 2300
ATEVGNVVKL NVQPTGPATI LIDKVEFSNG FYYLYSGDTF WKYNFDITDS
2310 2320 2330 2340 2350
KYTCKEALKN CSIITDFIVF NNNGSNVNQV KNACVYFSQM LCKPVKLVDS
2360 2370 2380 2390 2400
ALLASLSVDF GASLHSAFVS VLSNSFGKDL SSCNDMQDCK STLGFDDVPL
2410 2420 2430 2440 2450
DTFNAAVAEA HRYDVLLTDM SFNNFTTSYA KPEEKFPVHD IATCMRVGAK
2460 2470 2480 2490 2500
IVNHNVLVKD SIPVVWLVRD FIALSEETRK YIIRTTKVKG ITFMLTFNDC
2510 2520 2530 2540 2550
RMHTTIPTVC IANKKGAGLP SFSKVKKFFW FLCLFIVAAF FALSFLDFST
2560 2570 2580 2590 2600
QVSSDSDYDF KYIESGQLKT FDNPLSCVHN VFINFDQWHD AKFGFTPVNN
2610 2620 2630 2640 2650
PSCPIVVGVS DEARTVPGIP AGVYLAGKTL VFAINTIFGT SGLCFDASGV
2660 2670 2680 2690 2700
ADKGACIFNS ACTTLSGLGG TAVYCYKNGL VEGAKLYSEL APHSYYKMVD
2710 2720 2730 2740 2750
GNAVSLPEII SRGFGIRTIR TKAMTYCRVG QCVQSAEGVC FGADRFFVYN
2760 2770 2780 2790 2800
AESGSDFVCG TGLFTLLMNV ISVFSKTVPV TVLSGQILFN CIIAFVAVAV
2810 2820 2830 2840 2850
CFLFTKFKRM FGDMSVGVFT VGACTLLNNV SYIVTQNTLG MLGYATLYFL
2860 2870 2880 2890 2900
CTKGVRYMWI WHLGFLISYI LIAPWWVLMV YAFSAIFEFM PNLFKLKVST
2910 2920 2930 2940 2950
QLFEGDKFVG SFENAAAGTF VLDMHAYERL ANSISTEKLR QYASTYNKYK
2960 2970 2980 2990 3000
YYSGSASEAD YRLACFAHLA KAMMDYASNH NDTLYTPPTV SYNSTLQAGL
3010 3020 3030 3040 3050
RKMAQPSGVV EKCIVRVCYG NMALNGLWLG DIVMCPRHVI ASSTTSTIDY
3060 3070 3080 3090 3100
DYALSVLRLH NFSISSGNVF LGVVSATMRG ALLQIKVNQN NVHTPKYTYR
3110 3120 3130 3140 3150
TVRPGESFNI LACYDGAAAG VYGVNMRSNY TIRGSFINGA CGSPGYNINN
3160 3170 3180 3190 3200
GTVEFCYLHQ LELGSGCHVG SDLDGVMYGG YEDQPTLQVE GASSLFTENV
3210 3220 3230 3240 3250
LAFLYAALIN GSTWWLSSSR IAVDRFNEWA VHNGMTTVGN TDCFSILAAK
3260 3270 3280 3290 3300
TGVDVQRLLA SIQSLHKNFG GKQILGHTSL TDEFTTGEVV RQMYGVNLQG
3310 3320 3330 3340 3350
GYVSRACRNV LLVGSFLTFF WSELVSYTKF FWVNPGYVTP MFACLSLLSS
3360 3370 3380 3390 3400
LLMFTLKHKT LFFQVFLIPA LIVTSCINLA FDVEVYNYLA EHFDYHVSLM
3410 3420 3430 3440 3450
GFNAQGLVNI FVCFVVTILH GTYTWRFFNT PASSVTYVVA LLTAAYNYFY
3460 3470 3480 3490 3500
ASDILSCAMT LFASVTGNWF VGAVCYKVAV YMALRFPTFV AIFGDIKSVM
3510 3520 3530 3540 3550
FCYLVLGYFT CCFYGILYWF NRFFKVSVGV YDYTVSAAEF KYMVANGLRA
3560 3570 3580 3590 3600
PTGTLDSLLL SAKLIGIGGE RNIKISSVQS KLTDIKCSNV VLLGCLSSMN
3610 3620 3630 3640 3650
VSANSTEWAY CVDLHNKINL CNDPEKAQEM LLALLAFFLS KNSAFGLDDL
3660 3670 3680 3690 3700
LESYFNDNSM LQSVASTYVG LPSYVIYENA RQQYEDAVNN GSPPQLVKQL
3710 3720 3730 3740 3750
RHAMNVAKSE FDREASTQRK LDRMAEQAAA QMYKEARAVN RKSKVVSAMH
3760 3770 3780 3790 3800
SLLFGMLRRL DMSSVDTILN LAKDGVVPLS VIPAVSATKL NIVTSDIDSY
3810 3820 3830 3840 3850
NRIQREGCVH YAGTIWNIID IKDNDGKVVH VKEVTAQNAE SLSWPLVLGC
3860 3870 3880 3890 3900
ERIVKLQNNE IIPGKLKQRS IKAEGDGIVG EGKALYNNEG GRTFMYAFIS
3910 3920 3930 3940 3950
DKPDLRVVKW EFDGGCNTIE LEPPRKFLVD SPNGAQIKYL YFVRNLNTLR
3960 3970 3980 3990 4000
RGAVLGYIGA TVRLQAGKQT EQAINSSLLT LCAFAVDPAK TYIDAVKSGH
4010 4020 4030 4040 4050
KPVGNCVKML ANGSGNGQAV TNGVEASTNQ DSYGGASVCL YCRAHVEHPS
4060 4070 4080 4090 4100
MDGFCRLKGK YVQVPLGTVD PIRFVLENDV CKVCGCWLSN GCTCDRSIMQ
4110 4120 4130 4140 4150
STDMAYLNRV RGSSAARLEP CNGTDTQHVY RAFDIYNKDV ACLGKFLKVN
4160 4170 4180 4190 4200
CVRLKNLDKH DAFYVVKRCT KSAMEHEQSI YSRLEKCGAI AEHDFFTWKD
4210 4220 4230 4240 4250
GRAIYGNVCR KDLTEYTMMD LCYALRNFDE NNCDVLKSIL IKVGACEESY
4260 4270 4280 4290 4300
FNNKVWFDPV ENEDIHRVYA LLGTIVARAM LKCVKFCDAM VEQGIVGVVT
4310 4320 4330 4340 4350
LDNQDLNGDF YDFGDFTCSI KGMGVPICTS YYSYMMPVMG MTNCLASECF
4360 4370 4380 4390 4400
VKSDIFGEDF KSYDLLEYDF TEHKTALFNK YFKYWGLQYH PNCVDCSDEQ
4410 4420 4430 4440 4450
CIVHCANFNT LFSTTIPITA FGPLCRKCWI DGVPLVTTAG YHFKQLGIVW
4460 4470 4480 4490 4500
NNDLNLHSSR LSINELLQFC SDPALLIASS PALVDQRTVC FSVAALGTGM
4510 4520 4530 4540 4550
TNQTVKPGHF NKEFYDFLLE QGFFSEGSEL TLKHFFFAQK VDAAVKDFDY
4560 4570 4580 4590 4600
YRYNRPTVLD ICQARVVYQI VQRYFDIYEG GCITAKEVVV TNLNKSAGYP
4610 4620 4630 4640 4650
LNKFGKAGLY YESLSYEEQD ELYAYTKRNI LPTMTQLNLK YAISGKERAR
4660 4670 4680 4690 4700
TVGGVSLLST MTTRQYHQKH LKSIVNTRGA SVVIGTTKFY GGWDNMLKNL
4710 4720 4730 4740 4750
IDGVENPCLM GWDYPKCDRA LPNMIRMISA MILGSKHTTC CSSTDRFFRL
4760 4770 4780 4790 4800
CNELAQVLTE VVYSNGGFYL KPGGTTSGDA TTAYANSVFN IFQAVSANVN
4810 4820 4830 4840 4850
KLLSVDSNVC HNLEVKQLQR KLYECCYRST IVDDQFVVEY YGYLRKHFSM
4860 4870 4880 4890 4900
MILSDDGVVC YNNDYASLGY VADLNAFKAV LYYQNNVFMS ASKCWIEPDI
4910 4920 4930 4940 4950
NKGPHEFCSQ HTMQIVDKEG TYYLPYPDPS RILSAGVFVD DVVKTDAVVL
4960 4970 4980 4990 5000
LERYVSLAID AYPLSKHENP EYKKVFYVLL DWVKHLYKTL NAGVLESFSV
5010 5020 5030 5040 5050
TLLEDSTAKF WDESFYANMY EKSAVLQSAG LCVVCGSQTV LRCGDCLRRP
5060 5070 5080 5090 5100
MLCTKCAYDH VIGTTHKFIL AITPYVCCAS DCGVNDVTKL YLGGLSYWCH
5110 5120 5130 5140 5150
EHKPRLAFPL CSAGNVFGLY KNSATGSPDV EDFNRIATSD WTDVSDYRLA
5160 5170 5180 5190 5200
NDVKDSLRLF AAETIKAKEE SVKSSYACAT LHEVVGPKEL LLKWEVGRPK
5210 5220 5230 5240 5250
PPLNRNSVFT CYHITKNTKF QIGEFVFEKA EYDNDAVTYK TTATTKLVPG
5260 5270 5280 5290 5300
MVFVLTSHNV QPLRAPTIAN QERYSTIHKL HPAFNIPEAY SSLVPYYQLI
5310 5320 5330 5340 5350
GKQKITTIQG PPGSGKSHCV IGLGLYYPGA RIVFTACSHA AVDSLCVKAS
5360 5370 5380 5390 5400
TAYSNDKCSR IIPQRARVEC YDGFKSNNTS AQYLFSTVNA LPECNADIVV
5410 5420 5430 5440 5450
VDEVSMCTNY DLSVINQRIS YRHVVYVGDP QQLPAPRVMI SRGTLEPKDY
5460 5470 5480 5490 5500
NVVTQRMCAL KPDVFLHKCY RCPAEIVRTV SEMVYENQFI PVHPDSKQCF
5510 5520 5530 5540 5550
KIFCKGNVQV DNGSSINRRQ LDVVRMFLAK NPRWSKAVFI SPYNSQNYVA
5560 5570 5580 5590 5600
SRLLGLQIQT VDSSQGSEYD YVIYAQTSDT AHASNVNRFN VAITRAKKGI
5610 5620 5630 5640 5650
LCIMCDRSLF DLLKFFELKL SDLQANEGCG LFKDCSRGDD LLPPSHANTF
5660 5670 5680 5690 5700
MSLADNFKTD QYLAVQIGVN GPIKYEHVIS FMGFRFDINI PNHHTLFCTR
5710 5720 5730 5740 5750
DFAMRNVRGW LGFDVEGAHV VGSNVGTNVP LQLGFSNGVD FVVRPEGCVV
5760 5770 5780 5790 5800
TESGDYIKPV RARAPPGEQF AHLLPLLKRG QPWDVVRKRI VQMCSDYLAN
5810 5820 5830 5840 5850
LSDILIFVLW AGGLELTTMR YFVKIGPSKS CDCGKVATCY NSALHTYCCF
5860 5870 5880 5890 5900
KHALGCDYLY NPYCIDIQQW GYKGSLSLNH HEHCNVHRNE HVASGDAIMT
5910 5920 5930 5940 5950
RCLAIHDCFV KNVDWSITYP FIGNEAVINK SGRIVQSHTM RSVLKLYNPK
5960 5970 5980 5990 6000
AIYDIGNPKG IRCAVTDAKW FCFDKNPTNS NVKTLEYDYI THGQFDGLCL
6010 6020 6030 6040 6050
FWNCNVDMYP EFSVVCRFDT RCRSPLNLEG CNGGSLYVNN HAFHTPAFDK
6060 6070 6080 6090 6100
RAFAKLKPMP FFFYDDTECD KLQDSINYVP LRASNCITKC NVGGAVCSKH
6110 6120 6130 6140 6150
CAMYHSYVNA YNTFTSAGFT IWVPTSFDTY NLWQTFSNNL QGLENIAFNV
6160 6170 6180 6190 6200
LKKGSFVGDE GELPVAVVND KVLVRDGTVD TLVFTNKTSL PTNVAFELYA
6210 6220 6230 6240 6250
KRKVGLTPPI TILRNLGVVC TSKCVIWDYE AERPLTTFTK DVCKYTDFEG
6260 6270 6280 6290 6300
DVCTLFDNSI VGSLERFSMT QNAVLMSLTA VKKLTGIKLT YGYLNGVPVN
6310 6320 6330 6340 6350
THEDKPFTWY IYTRKNGKFE DYPDGYFTQG RTTADFSPRS DMEKDFLSMD
6360 6370 6380 6390 6400
MGLFINKYGL EDYGFEHVVY GDVSKTTLGG LHLLISQVRL ACMGVLKIDE
6410 6420 6430 6440 6450
FVSSNDSTLK SCTVTYADNP SSKMVCTYMD LLLDDFVSIL KSLDLSVVSK
6460 6470 6480 6490 6500
VHEVMVDCKM WRWMLWCKDH KLQTFYPQLQ ASEWKCGYSM PSIYKIQRMC
6510 6520 6530 6540 6550
LEPCNLYNYG AGVKLPDGIM FNVVKYTQLC QYLNSTTMCV PHHMRVLHLG
6560 6570 6580 6590 6600
AGSDKGVAPG TAVLRRWLPL DAIIVDNDSV DYVSDADYSV TGDCSTLYLS
6610 6620 6630 6640 6650
DKFDLVISDM YDGKIKSCDG ENVSKEGFFP YINGVITEKL ALGGTVAIKV
6660 6670 6680 6690 6700
TEFSWNKKLY ELIQKFEYWT MFCTSVNTSS SEAFLIGVHY LGDFASGAVI
6710 6720 6730 6740 6750
DGNTMHANYI FWRNSTIMTM SYNSVLDLSK FNCKHKATVV VNLKDSSISD
6760 6770 6780
VVLGLLKNGK LLVRNNDAIC GFSNHLVNVN K

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:6,781
Mass (Da):753,158
Last modified:June 10, 2008 - v1
Checksum:iC5E29CBA752AD5BB
GO
Isoform Replicase polyprotein 1a (identifier: P0C6V6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6V6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,117
Mass (Da):452,818
GO

Sequence cautioni

The sequence AAK38661.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353511 Genomic RNA. Translation: AAK38661.1. Sequence problems.
RefSeqiNP_598309.2. NC_003436.1.

Genome annotation databases

GeneIDi935181.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353511 Genomic RNA. Translation: AAK38661.1 . Sequence problems.
RefSeqi NP_598309.2. NC_003436.1.

3D structure databases

ProteinModelPortali P0C6Y4.
SMRi P0C6Y4. Positions 2998-3297, 3860-3964, 3972-4096.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 935181.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR011050. Pectin_lyase_fold/virulence.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view ]
SMARTi SM00506. A1pp. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF51126. SSF51126. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Further analysis of the genome of porcine epidemic diarrhea virus."
    Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
    Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
    Kocherhans R., Bridgen A., Ackermann M., Tobler K.
    Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1AB_PEDV7
AccessioniPrimary (citable) accession number: P0C6Y4
Secondary accession number(s): Q91AV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3