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P0C6Y4

- R1AB_PEDV7

UniProt

P0C6Y4 - R1AB_PEDV7

Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Porcine epidemic diarrhea virus (strain CV777) (PEDV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
    The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.By similarity
    The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
    ATP + H2O = ADP + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei110 – 1112Cleavage; by PL1-PROBy similarity
    Sitei895 – 8962Cleavage; by PL1-PROBy similarity
    Active sitei1091 – 10911For PL1-PRO activityPROSITE-ProRule annotation
    Active sitei1239 – 12391For PL1-PRO activityPROSITE-ProRule annotation
    Active sitei1729 – 17291For PL2-PRO activityPROSITE-ProRule annotation
    Active sitei1888 – 18881For PL2-PRO activityPROSITE-ProRule annotation
    Sitei2516 – 25172Cleavage; by PL2-PROBy similarity
    Sitei2997 – 29982Cleavage; by 3CL-PROBy similarity
    Active sitei3038 – 30381For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3141 – 31411For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3299 – 33002Cleavage; by 3CL-PROBy similarity
    Sitei3579 – 35802Cleavage; by 3CL-PROBy similarity
    Sitei3662 – 36632Cleavage; by 3CL-PROBy similarity
    Sitei3857 – 38582Cleavage; by 3CL-PROBy similarity
    Sitei3965 – 39662Cleavage; by 3CL-PROBy similarity
    Sitei4100 – 41012Cleavage; by 3CL-PROBy similarity
    Sitei5027 – 50282Cleavage; by 3CL-PROBy similarity
    Sitei5546 – 55472Cleavage; by 3CL-PROBy similarity
    Sitei6141 – 61422Cleavage; by 3CL-PROBy similarity
    Sitei6480 – 64812Cleavage; by 3CL-PROBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1162 – 119332C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4039 – 405517By similarityAdd
    BLAST
    Zinc fingeri4081 – 409414By similarityAdd
    BLAST
    Nucleotide bindingi5310 – 53178ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. helicase activity Source: UniProtKB-KW
    6. methyltransferase activity Source: InterPro
    7. omega peptidase activity Source: InterPro
    8. RNA binding Source: UniProtKB-KW
    9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    10. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of host autophagy Source: UniProtKB-KW
    2. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    3. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    4. transcription, DNA-templated Source: InterPro
    5. viral genome replication Source: InterPro
    6. viral protein processing Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Activation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Short name:
    pp1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 15 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    p9
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p87
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PLP1/PLP2
    Papain-like proteinases 1/2
    p195
    Non-structural protein 4
    Short name:
    nsp4
    Alternative name(s):
    Peptide HD2
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    M-PRO
    nsp5
    p34
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Alternative name(s):
    p5
    Non-structural protein 8
    Short name:
    nsp8
    Alternative name(s):
    p23
    Non-structural protein 9
    Short name:
    nsp9
    Alternative name(s):
    p12
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    p14
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    nsp12
    p100
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    nsp13
    p66
    p66-HEL
    Exoribonuclease (EC:3.1.13.-)
    Short name:
    ExoN
    Alternative name(s):
    nsp14
    Alternative name(s):
    NendoU
    nsp15
    Alternative name(s):
    nsp16
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiPorcine epidemic diarrhea virus (strain CV777) (PEDV)
    Taxonomic identifieri229032 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008159: Genome

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
    Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. host cell membrane Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 110110Non-structural protein 1By similarityPRO_0000037369Add
    BLAST
    Chaini111 – 895785Non-structural protein 2By similarityPRO_0000037370Add
    BLAST
    Chaini896 – 25161621Non-structural protein 3By similarityPRO_0000037371Add
    BLAST
    Chaini2517 – 2997481Non-structural protein 4By similarityPRO_0000037372Add
    BLAST
    Chaini2998 – 32993023C-like proteinaseBy similarityPRO_0000037373Add
    BLAST
    Chaini3300 – 3579280Non-structural protein 6By similarityPRO_0000037374Add
    BLAST
    Chaini3580 – 366283Non-structural protein 7By similarityPRO_0000037375Add
    BLAST
    Chaini3663 – 3857195Non-structural protein 8By similarityPRO_0000037376Add
    BLAST
    Chaini3858 – 3965108Non-structural protein 9By similarityPRO_0000037377Add
    BLAST
    Chaini3966 – 4100135Non-structural protein 10By similarityPRO_0000037378Add
    BLAST
    Chaini4101 – 5027927RNA-directed RNA polymeraseBy similarityPRO_0000037379Add
    BLAST
    Chaini5028 – 5546519HelicasePRO_0000037380Add
    BLAST
    Chaini5547 – 6141595ExoribonucleaseBy similarityPRO_0000037381Add
    BLAST
    Chaini6142 – 6480339Uridylate-specific endoribonucleaseBy similarityPRO_0000037382Add
    BLAST
    Chaini6481 – 6781301Putative 2'-O-methyl transferaseBy similarityPRO_0000037383Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C6Y4.
    SMRiP0C6Y4. Positions 2998-3297, 3860-3964, 3972-4096.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1959 – 197921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2022 – 204221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2105 – 212521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2127 – 214721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2150 – 217021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2528 – 254821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2619 – 263921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2654 – 267421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2754 – 277421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2787 – 280721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2814 – 283421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2863 – 288321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3336 – 335621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3361 – 338121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3399 – 341921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3431 – 345121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3454 – 347421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3476 – 349621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3500 – 352021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1057 – 1296240Peptidase C16 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1297 – 1465169MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1691 – 1951261Peptidase C16 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini2998 – 3299302Peptidase C30PROSITE-ProRule annotationAdd
    BLAST
    Domaini4707 – 4869163RdRp catalyticAdd
    BLAST
    Domaini5028 – 511184CV MBDAdd
    BLAST
    Domaini5275 – 5466192(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini5467 – 5636170(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1959 – 2170212HD1Add
    BLAST
    Regioni2528 – 2883356HD2Add
    BLAST
    Regioni3336 – 3520185HD3Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1816 – 1932117Val-richAdd
    BLAST

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1162 – 119332C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4039 – 405517By similarityAdd
    BLAST
    Zinc fingeri4081 – 409414By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR009466. NSP11.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR011050. Pectin_lyase_fold/virulence.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 2 hits.
    [Graphical view]
    SMARTiSM00506. A1pp. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF51126. SSF51126. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 2 hits.
    PS51657. PSRV_HELICASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1ab (identifier: P0C6Y4-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MASNHVTLAF ANDAEISAFG FCTASEAVSY YSEAAASGFM QCRFVSLDLA     50
    DTVEGLLPED YVMVVIGTTK LSAYVDTFGS RPRNICGWLL FSNCNYFLEE 100
    LELTFGRRGG NIVPVDQYMC GADGKPVLQE SEWEYTDFFA DSEDGQLNIA 150
    GITYVKAWIV ERSDVSYASQ NLTSIKSITY CSTYEHTFLD GTAMKVARTP 200
    KIKKNVVLSE PLATIYREIG SPFVDNGSDA RSIIRRPVFL HAFVKCKCGS 250
    YHWTVGDWTS YVSTCCGFKC KPVLVASCSA MPGSVVVTRA GAGTGVKYYN 300
    NMFLRHVADI DGLAFWRILK VQSKDDLACS GKFLEHHEEG FTDPCYFLND 350
    SSLATKLKFD ILSGKFSDEV KQAIIAGHVV VGSALVDIVD DALGQPWFIR 400
    KLGDLASAPW EQLKAVVRGL GLLSDEVVLF GKRLSCATLS IVNGVFEFLA 450
    DVPEKLAAAV TVFVNFLNEF FESACDCLKV GGKTFNKVGS YVLFDNALVK 500
    LVKAKARGPR QAGICEVRYT SLVVGSTTKV VSKRVENANV NLVVVDEDVT 550
    LNTTGRTVVV DGLAFFESDG FYRHLADADV VIEHPVYKSA CELKPVFECD 600
    PIPDFPLPVA ASVAELCVQT DLLLKNYNTP YKTYSCVVRG DKCCITCTLQ 650
    FKAPSYVEDA VNFVDLCTKN IGTAGFHEFY ITAHEQQDLQ GFLTTCCTMS 700
    GFECFMPTIP QCPAVLEEID GGSIWRSFIT GLNTMWDFCK RLKVSFGLDG 750
    IVVTVARKFK RLGALLAEMY NTYLSTVVEN LVLAGVSFKY YATSVPKIVL 800
    GGCFHSVKSV FASVFQIPVQ AGIEKFKVFL NCVHPVVPRV IETSFVELEE 850
    TTFKPPALNG GIAIVDGFAF YYDGTLYYPT DGNSVVPICF KKKGGGDVKF 900
    SDEVSVKTID PVYKVSLEFE FESETIMAVL NKAVGNRIKV TGGWDDVVEY 950
    INVAIEVLKD HVEVPKYYIY DEEGGTDPNL PVMVSQWPLN DDTISQDLLD 1000
    VEVVTDAPID SEGDEVDSSA PEKVADVANS EPGDDGLPVA PETNVESEVE 1050
    EVAATLSFIK DTPSTVTKDP FAFDFVSYGG LKVLRQSHNN CWVTSTLVQL 1100
    QLLGIVDDPA MELFSAGRVG PMVRKCYESQ KAILGSLGDV SACLESLTKD 1150
    LHTLKITCSV VCGCGTGERI YEGCAFRMTP TLEPFPYGAC AQCAQVLMHT 1200
    FKSIVGTGIF CRDTTALSLD SLVVKPLCAA AFIGKDSGHY VTNFYDAAMA 1250
    IDGYGRHQIK YDTLNTICVK DVNWTAPLVP AVDSVVEPVV KPFYSYKNVD 1300
    FYQGDFSDLV KLPCDFVVNA ANEKLSHGGG IAKAIDVYTK GMLQKCSNDY 1350
    IKAHGPIKVG RGVMLEALGL KVFNVVGPRK GKHAPELLVK AYKSVFANSG 1400
    VALTPLISVG IFSVPLEESL SAFLACVGDR HCKCFCYGDK EREAIIKYMD 1450
    GLVDAIFKEA LVDTTPVQED VQQVSQKPVL PNFEPFRIEG AHAFYECNPE 1500
    GLMSLGADKL VLFTNSNLDF CSVGKCLNDV TSGALLEAIN VFKKSNKTVP 1550
    AGNCVTLDCA NMISITMVVL PFDGDANYDK NYARAVVKVS KLKGKLVLAV 1600
    DDATLYSKLS HLSVLGFVST PDDVERFYAN KSVVIKVTED TRSVKAVKVE 1650
    STATYGQQIG PCLVNDTVVT DNKPVVADVV AKVVPNANWD SHYGFDKAGE 1700
    FHMLDHTGFT FPSEVVNGRR VIKTTDNNCW VNVTCLQLQF ARFRFKSAGL 1750
    QAMWESYCTG DVAMFVHWLY WLTGVDKGQP SDSENALNML SKYIVPAGSV 1800
    TIERVTHDGC CCSKRVVTAP VVNASVLKLG VEDGLCPHGL NYIGKVVVVK 1850
    GTTIVVNVGK PVVAPSHLFL KGVSYTTFLD NGNGVVGHYT VFDHGTGMVH 1900
    DGDAFVPGDL NVSPVTNVVV SEQTAVVIKD PVKKAELDAT KLLDTMNYAS 1950
    ERFFSFGDFM SRNLITVFLY ILSILGLCFR AFRKRDVKVL AGVPQRTGII 2000
    LRKSMRYNAK ALGVFFKLKL YWFKVLGKFS LGIYALYALL FMTIRFTPIG 2050
    SPVCDDVVAG YANSSFDKNE YCNSVICKVC LYGYQELSDF SHTQVVWQHL 2100
    RDPLIGNVMP FFYLAFLAIF GGVYVKAITL YFIFQYLNSL GVFLGLQQSI 2150
    WFLQLVPFDV FGDEIVVFFI VTRVLMFIKH VCLGCDKASC VACSKSARLK 2200
    RVPVQTIFQG TSKSFYVHAN GGSKFCKKHN FFCLNCDSYG PGCTFINDVI 2250
    ATEVGNVVKL NVQPTGPATI LIDKVEFSNG FYYLYSGDTF WKYNFDITDS 2300
    KYTCKEALKN CSIITDFIVF NNNGSNVNQV KNACVYFSQM LCKPVKLVDS 2350
    ALLASLSVDF GASLHSAFVS VLSNSFGKDL SSCNDMQDCK STLGFDDVPL 2400
    DTFNAAVAEA HRYDVLLTDM SFNNFTTSYA KPEEKFPVHD IATCMRVGAK 2450
    IVNHNVLVKD SIPVVWLVRD FIALSEETRK YIIRTTKVKG ITFMLTFNDC 2500
    RMHTTIPTVC IANKKGAGLP SFSKVKKFFW FLCLFIVAAF FALSFLDFST 2550
    QVSSDSDYDF KYIESGQLKT FDNPLSCVHN VFINFDQWHD AKFGFTPVNN 2600
    PSCPIVVGVS DEARTVPGIP AGVYLAGKTL VFAINTIFGT SGLCFDASGV 2650
    ADKGACIFNS ACTTLSGLGG TAVYCYKNGL VEGAKLYSEL APHSYYKMVD 2700
    GNAVSLPEII SRGFGIRTIR TKAMTYCRVG QCVQSAEGVC FGADRFFVYN 2750
    AESGSDFVCG TGLFTLLMNV ISVFSKTVPV TVLSGQILFN CIIAFVAVAV 2800
    CFLFTKFKRM FGDMSVGVFT VGACTLLNNV SYIVTQNTLG MLGYATLYFL 2850
    CTKGVRYMWI WHLGFLISYI LIAPWWVLMV YAFSAIFEFM PNLFKLKVST 2900
    QLFEGDKFVG SFENAAAGTF VLDMHAYERL ANSISTEKLR QYASTYNKYK 2950
    YYSGSASEAD YRLACFAHLA KAMMDYASNH NDTLYTPPTV SYNSTLQAGL 3000
    RKMAQPSGVV EKCIVRVCYG NMALNGLWLG DIVMCPRHVI ASSTTSTIDY 3050
    DYALSVLRLH NFSISSGNVF LGVVSATMRG ALLQIKVNQN NVHTPKYTYR 3100
    TVRPGESFNI LACYDGAAAG VYGVNMRSNY TIRGSFINGA CGSPGYNINN 3150
    GTVEFCYLHQ LELGSGCHVG SDLDGVMYGG YEDQPTLQVE GASSLFTENV 3200
    LAFLYAALIN GSTWWLSSSR IAVDRFNEWA VHNGMTTVGN TDCFSILAAK 3250
    TGVDVQRLLA SIQSLHKNFG GKQILGHTSL TDEFTTGEVV RQMYGVNLQG 3300
    GYVSRACRNV LLVGSFLTFF WSELVSYTKF FWVNPGYVTP MFACLSLLSS 3350
    LLMFTLKHKT LFFQVFLIPA LIVTSCINLA FDVEVYNYLA EHFDYHVSLM 3400
    GFNAQGLVNI FVCFVVTILH GTYTWRFFNT PASSVTYVVA LLTAAYNYFY 3450
    ASDILSCAMT LFASVTGNWF VGAVCYKVAV YMALRFPTFV AIFGDIKSVM 3500
    FCYLVLGYFT CCFYGILYWF NRFFKVSVGV YDYTVSAAEF KYMVANGLRA 3550
    PTGTLDSLLL SAKLIGIGGE RNIKISSVQS KLTDIKCSNV VLLGCLSSMN 3600
    VSANSTEWAY CVDLHNKINL CNDPEKAQEM LLALLAFFLS KNSAFGLDDL 3650
    LESYFNDNSM LQSVASTYVG LPSYVIYENA RQQYEDAVNN GSPPQLVKQL 3700
    RHAMNVAKSE FDREASTQRK LDRMAEQAAA QMYKEARAVN RKSKVVSAMH 3750
    SLLFGMLRRL DMSSVDTILN LAKDGVVPLS VIPAVSATKL NIVTSDIDSY 3800
    NRIQREGCVH YAGTIWNIID IKDNDGKVVH VKEVTAQNAE SLSWPLVLGC 3850
    ERIVKLQNNE IIPGKLKQRS IKAEGDGIVG EGKALYNNEG GRTFMYAFIS 3900
    DKPDLRVVKW EFDGGCNTIE LEPPRKFLVD SPNGAQIKYL YFVRNLNTLR 3950
    RGAVLGYIGA TVRLQAGKQT EQAINSSLLT LCAFAVDPAK TYIDAVKSGH 4000
    KPVGNCVKML ANGSGNGQAV TNGVEASTNQ DSYGGASVCL YCRAHVEHPS 4050
    MDGFCRLKGK YVQVPLGTVD PIRFVLENDV CKVCGCWLSN GCTCDRSIMQ 4100
    STDMAYLNRV RGSSAARLEP CNGTDTQHVY RAFDIYNKDV ACLGKFLKVN 4150
    CVRLKNLDKH DAFYVVKRCT KSAMEHEQSI YSRLEKCGAI AEHDFFTWKD 4200
    GRAIYGNVCR KDLTEYTMMD LCYALRNFDE NNCDVLKSIL IKVGACEESY 4250
    FNNKVWFDPV ENEDIHRVYA LLGTIVARAM LKCVKFCDAM VEQGIVGVVT 4300
    LDNQDLNGDF YDFGDFTCSI KGMGVPICTS YYSYMMPVMG MTNCLASECF 4350
    VKSDIFGEDF KSYDLLEYDF TEHKTALFNK YFKYWGLQYH PNCVDCSDEQ 4400
    CIVHCANFNT LFSTTIPITA FGPLCRKCWI DGVPLVTTAG YHFKQLGIVW 4450
    NNDLNLHSSR LSINELLQFC SDPALLIASS PALVDQRTVC FSVAALGTGM 4500
    TNQTVKPGHF NKEFYDFLLE QGFFSEGSEL TLKHFFFAQK VDAAVKDFDY 4550
    YRYNRPTVLD ICQARVVYQI VQRYFDIYEG GCITAKEVVV TNLNKSAGYP 4600
    LNKFGKAGLY YESLSYEEQD ELYAYTKRNI LPTMTQLNLK YAISGKERAR 4650
    TVGGVSLLST MTTRQYHQKH LKSIVNTRGA SVVIGTTKFY GGWDNMLKNL 4700
    IDGVENPCLM GWDYPKCDRA LPNMIRMISA MILGSKHTTC CSSTDRFFRL 4750
    CNELAQVLTE VVYSNGGFYL KPGGTTSGDA TTAYANSVFN IFQAVSANVN 4800
    KLLSVDSNVC HNLEVKQLQR KLYECCYRST IVDDQFVVEY YGYLRKHFSM 4850
    MILSDDGVVC YNNDYASLGY VADLNAFKAV LYYQNNVFMS ASKCWIEPDI 4900
    NKGPHEFCSQ HTMQIVDKEG TYYLPYPDPS RILSAGVFVD DVVKTDAVVL 4950
    LERYVSLAID AYPLSKHENP EYKKVFYVLL DWVKHLYKTL NAGVLESFSV 5000
    TLLEDSTAKF WDESFYANMY EKSAVLQSAG LCVVCGSQTV LRCGDCLRRP 5050
    MLCTKCAYDH VIGTTHKFIL AITPYVCCAS DCGVNDVTKL YLGGLSYWCH 5100
    EHKPRLAFPL CSAGNVFGLY KNSATGSPDV EDFNRIATSD WTDVSDYRLA 5150
    NDVKDSLRLF AAETIKAKEE SVKSSYACAT LHEVVGPKEL LLKWEVGRPK 5200
    PPLNRNSVFT CYHITKNTKF QIGEFVFEKA EYDNDAVTYK TTATTKLVPG 5250
    MVFVLTSHNV QPLRAPTIAN QERYSTIHKL HPAFNIPEAY SSLVPYYQLI 5300
    GKQKITTIQG PPGSGKSHCV IGLGLYYPGA RIVFTACSHA AVDSLCVKAS 5350
    TAYSNDKCSR IIPQRARVEC YDGFKSNNTS AQYLFSTVNA LPECNADIVV 5400
    VDEVSMCTNY DLSVINQRIS YRHVVYVGDP QQLPAPRVMI SRGTLEPKDY 5450
    NVVTQRMCAL KPDVFLHKCY RCPAEIVRTV SEMVYENQFI PVHPDSKQCF 5500
    KIFCKGNVQV DNGSSINRRQ LDVVRMFLAK NPRWSKAVFI SPYNSQNYVA 5550
    SRLLGLQIQT VDSSQGSEYD YVIYAQTSDT AHASNVNRFN VAITRAKKGI 5600
    LCIMCDRSLF DLLKFFELKL SDLQANEGCG LFKDCSRGDD LLPPSHANTF 5650
    MSLADNFKTD QYLAVQIGVN GPIKYEHVIS FMGFRFDINI PNHHTLFCTR 5700
    DFAMRNVRGW LGFDVEGAHV VGSNVGTNVP LQLGFSNGVD FVVRPEGCVV 5750
    TESGDYIKPV RARAPPGEQF AHLLPLLKRG QPWDVVRKRI VQMCSDYLAN 5800
    LSDILIFVLW AGGLELTTMR YFVKIGPSKS CDCGKVATCY NSALHTYCCF 5850
    KHALGCDYLY NPYCIDIQQW GYKGSLSLNH HEHCNVHRNE HVASGDAIMT 5900
    RCLAIHDCFV KNVDWSITYP FIGNEAVINK SGRIVQSHTM RSVLKLYNPK 5950
    AIYDIGNPKG IRCAVTDAKW FCFDKNPTNS NVKTLEYDYI THGQFDGLCL 6000
    FWNCNVDMYP EFSVVCRFDT RCRSPLNLEG CNGGSLYVNN HAFHTPAFDK 6050
    RAFAKLKPMP FFFYDDTECD KLQDSINYVP LRASNCITKC NVGGAVCSKH 6100
    CAMYHSYVNA YNTFTSAGFT IWVPTSFDTY NLWQTFSNNL QGLENIAFNV 6150
    LKKGSFVGDE GELPVAVVND KVLVRDGTVD TLVFTNKTSL PTNVAFELYA 6200
    KRKVGLTPPI TILRNLGVVC TSKCVIWDYE AERPLTTFTK DVCKYTDFEG 6250
    DVCTLFDNSI VGSLERFSMT QNAVLMSLTA VKKLTGIKLT YGYLNGVPVN 6300
    THEDKPFTWY IYTRKNGKFE DYPDGYFTQG RTTADFSPRS DMEKDFLSMD 6350
    MGLFINKYGL EDYGFEHVVY GDVSKTTLGG LHLLISQVRL ACMGVLKIDE 6400
    FVSSNDSTLK SCTVTYADNP SSKMVCTYMD LLLDDFVSIL KSLDLSVVSK 6450
    VHEVMVDCKM WRWMLWCKDH KLQTFYPQLQ ASEWKCGYSM PSIYKIQRMC 6500
    LEPCNLYNYG AGVKLPDGIM FNVVKYTQLC QYLNSTTMCV PHHMRVLHLG 6550
    AGSDKGVAPG TAVLRRWLPL DAIIVDNDSV DYVSDADYSV TGDCSTLYLS 6600
    DKFDLVISDM YDGKIKSCDG ENVSKEGFFP YINGVITEKL ALGGTVAIKV 6650
    TEFSWNKKLY ELIQKFEYWT MFCTSVNTSS SEAFLIGVHY LGDFASGAVI 6700
    DGNTMHANYI FWRNSTIMTM SYNSVLDLSK FNCKHKATVV VNLKDSSISD 6750
    VVLGLLKNGK LLVRNNDAIC GFSNHLVNVN K 6781

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:6,781
    Mass (Da):753,158
    Last modified:June 10, 2008 - v1
    Checksum:iC5E29CBA752AD5BB
    GO
    Isoform Replicase polyprotein 1a (identifier: P0C6V6-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform can be found in the external entry P0C6V6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by conventional translation.

    Length:4,117
    Mass (Da):452,818
    GO

    Sequence cautioni

    The sequence AAK38661.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF353511 Genomic RNA. Translation: AAK38661.1. Sequence problems.
    RefSeqiNP_598309.2. NC_003436.1.

    Genome annotation databases

    GeneIDi935181.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF353511 Genomic RNA. Translation: AAK38661.1 . Sequence problems.
    RefSeqi NP_598309.2. NC_003436.1.

    3D structure databases

    ProteinModelPortali P0C6Y4.
    SMRi P0C6Y4. Positions 2998-3297, 3860-3964, 3972-4096.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 935181.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR009466. NSP11.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR011050. Pectin_lyase_fold/virulence.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF06478. Corona_RPol_N. 1 hit.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 2 hits.
    [Graphical view ]
    SMARTi SM00506. A1pp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF51126. SSF51126. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEi PS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 2 hits.
    PS51657. PSRV_HELICASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Further analysis of the genome of porcine epidemic diarrhea virus."
      Bridgen A., Kocherhans R., Tobler K., Carvajal A., Ackermann M.
      Adv. Exp. Med. Biol. 440:781-786(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence."
      Kocherhans R., Bridgen A., Ackermann M., Tobler K.
      Virus Genes 23:137-144(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiR1AB_PEDV7
    AccessioniPrimary (citable) accession number: P0C6Y4
    Secondary accession number(s): Q91AV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3