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P0C6Y0 (R1AB_CVMJH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p28
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PL1/PL2
    Papain-like proteinases 1/2
    p210
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
    p44
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p27
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p10
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p22
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p15
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
    p100
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
    p67
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
    p35
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus) [Complete proteome]
Taxonomic identifier11144 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length7180 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential Ref.6.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential Ref.6.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential Ref.6.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity Ref.6. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Ref.6

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity Ref.6. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Ref.6

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity Ref.6. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Ref.6

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity Ref.6. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Ref.6

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity. Ref.6

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity Ref.6.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Sequence caution

The sequence AAA46457.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAA46457.1 differs from that shown. Reason: Frameshift at positions 690, 915, 1529, 2069 and 3316.

The sequence AAA46458.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host translation shutoff by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6Y0-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6V1-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6V1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247Non-structural protein 1 Probable
PRO_0000037354
Chain248 – 832585Non-structural protein 2 Probable
PRO_0000037355
Chain833 – 28402008Non-structural protein 3 Probable
PRO_0000037356
Chain2841 – 3336496Non-structural protein 4 Probable
PRO_0000037357
Chain3337 – 36393033C-like proteinase Probable
PRO_0000037358
Chain3640 – 3927288Non-structural protein 6 Probable
PRO_0000037359
Chain3928 – 401992Non-structural protein 7 Probable
PRO_0000037360
Chain4020 – 4213194Non-structural protein 8
PRO_0000037361
Chain4214 – 4323110Non-structural protein 9 Probable
PRO_0000037362
Chain4324 – 4460137Non-structural protein 10 Probable
PRO_0000037363
Chain4461 – 5388928RNA-directed RNA polymerase Probable
PRO_0000037364
Chain5389 – 5988600Helicase Probable
PRO_0000037365
Chain5989 – 6507519Exoribonuclease By similarity
PRO_0000037366
Chain6508 – 6881374Uridylate-specific endoribonuclease By similarity
PRO_0000037367
Chain6882 – 7180299Putative 2'-O-methyl transferase By similarity
PRO_0000037368

Regions

Transmembrane2228 – 224821Helical; Potential
Transmembrane2289 – 230921Helical; Potential
Transmembrane2320 – 234021Helical; Potential
Transmembrane2403 – 242321Helical; Potential
Transmembrane2445 – 246521Helical; Potential
Transmembrane2846 – 286621Helical; Potential
Transmembrane3099 – 311921Helical; Potential
Transmembrane3121 – 314121Helical; Potential
Transmembrane3153 – 317321Helical; Potential
Transmembrane3180 – 320021Helical; Potential
Transmembrane3205 – 322521Helical; Potential
Transmembrane3648 – 366821Helical; Potential
Transmembrane3678 – 369821Helical; Potential
Transmembrane3705 – 372521Helical; Potential
Transmembrane3748 – 376821Helical; Potential
Transmembrane3775 – 379521Helical; Potential
Transmembrane3802 – 382221Helical; Potential
Transmembrane3846 – 386621Helical; Potential
Domain1083 – 1320238Peptidase C16 1
Domain1321 – 1481161Macro
Domain1677 – 1936260Peptidase C16 2
Domain3337 – 3639303Peptidase C30
Domain5068 – 5230163RdRp catalytic
Domain5389 – 547284CV MBD
Domain5644 – 5825182(+)RNA virus helicase ATP-binding
Domain5826 – 6003178(+)RNA virus helicase C-terminal
Zinc finger1197 – 122529C4-type 1
Zinc finger1793 – 182937C4-type 2
Zinc finger4397 – 441317 By similarity
Zinc finger4439 – 445214 By similarity
Nucleotide binding5669 – 56768ATP By similarity
Region2228 – 2465238HD1
Region2846 – 3225380HD2
Region3648 – 3866219HD3

Sites

Active site11201For PL1-PRO activity By similarity
Active site12711For PL1-PRO activity By similarity
Active site17151For PL2-PRO activity By similarity
Active site18721For PL2-PRO activity By similarity
Active site33771For 3CL-PRO activity By similarity
Active site34811For 3CL-PRO activity By similarity
Site247 – 2482Cleavage; by PL1-PRO Probable
Site832 – 8332Cleavage; by PL1-PRO Probable
Site2840 – 28412Cleavage; by PL2-PRO Probable
Site3336 – 33372Cleavage; by 3CL-PRO Probable
Site3639 – 36402Cleavage; by 3CL-PRO Probable
Site3927 – 39282Cleavage; by 3CL-PRO Probable
Site4019 – 40202Cleavage; by 3CL-PRO Probable
Site4213 – 42142Cleavage; by 3CL-PRO Probable
Site4323 – 43242Cleavage; by 3CL-PRO Probable
Site4460 – 44612Cleavage; by 3CL-PRO Probable
Site5388 – 53892Cleavage; by 3CL-PRO Probable
Site5988 – 59892Cleavage; by 3CL-PRO Probable
Site6507 – 65082Cleavage; by 3CL-PRO Probable
Site6881 – 68822Cleavage; by 3CL-PRO Probable

Experimental info

Mutagenesis28351F → A: No processing between p210 and peptide HD2. Ref.5
Mutagenesis28361S → A: No effect. Ref.5
Mutagenesis28371L → A: No effect. Ref.5
Mutagenesis28381K → A: No effect. Ref.5
Mutagenesis28381K → N: No effect. Ref.5
Mutagenesis28391G → A: Partial processing between p210 and peptide HD2. Ref.5
Mutagenesis28391G → N: No processing between p210 and peptide HD2. Ref.5
Mutagenesis28391G → V: No processing between p210 and peptide HD2. Ref.5
Mutagenesis28401G → A: No processing between p210 and peptide HD2. Ref.5
Mutagenesis28401G → N: No processing between p210 and peptide HD2. Ref.5
Mutagenesis28401G → V: No processing between p210 and peptide HD2. Ref.5
Mutagenesis28411A → N: No effect. Ref.5
Mutagenesis28421V → N or M: No effect. Ref.5
Mutagenesis28461V → M: No effect. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 313A78E1CC87B347

FASTA7,180803,440
        10         20         30         40         50         60 
MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK GKTLVNHVRV 

        70         80         90        100        110        120 
DCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG SAVLVKPSKR LSVQAWAKLG 

       130        140        150        160        170        180 
VLPKTPAMGL FKRFCLCNTR ECVCDAHVAF QLFTVQPDGV CLGNGRFIGW FVPVTAIPEY 

       190        200        210        220        230        240 
AKQWLQPWSI LLRKGGNKGS VTSGHFRRAV TMPVYDFNVE DACEEVHLNP RGKYSCKAYA 

       250        260        270        280        290        300 
LLRGYRGVKP ILFVDQYGCD YTGCLAKGLE DYGDLTLSEM KELSPVWRDS LDNEVVVAWH 

       310        320        330        340        350        360 
VDRDPRAVMR LQTLATVRSI EYVGQPIEDM VDGDVVMREP AHLLAPNAIV KRLPRLVETM 

       370        380        390        400        410        420 
LYTDSSVTEF CYKTKLCDCG FITQFGYVDC CGDTCGFRGW VPGNMMDGFP CPGCCKSYMP 

       430        440        450        460        470        480 
WELEAQSSGV IPEGGVLFTQ STDTVNRESF KLYGHAVVPF GGAAYWSPYP GMWLPVIWSS 

       490        500        510        520        530        540 
VKSYSYLTYT GVVGCKAIVQ ETDAICRFLY MDYVQHKCGN LEQRAILGLD DVYHRQLLVN 

       550        560        570        580        590        600 
RGDYSLLLEN VDLFVKRRAE FACKFATCGD GLVPLLLDGL VPRSYYLIKS GQAFTSLMVN 

       610        620        630        640        650        660 
FSREVVDMCM DMALLFMHDV KVATKYVKKV TGKVAVRFKA LGIAVVRKIT EWFDLAVDTA 

       670        680        690        700        710        720 
ASAAGWLCYQ LVNGLFAVAN GVITFIQEVP ELVKNFVDKF KTFFKVLIDS MSVSILSGLT 

       730        740        750        760        770        780 
VVKTASNRVC LAGSKVYEVV QKSLPAYIMP VGCSEATCLV GEIEPAVFED DVVDVVKAPL 

       790        800        810        820        830        840 
TYQGCCKPPS SFEKICIVDK LYMAKCGDQF YPVVVDNDTV GVLDQCWRFP CAGKKVVFND 

       850        860        870        880        890        900 
KPKVKEVPST RKIKIIFALD ATFDSVLSKA CSEFEVDKDV TLDELLDVVL DAVESTLSPC 

       910        920        930        940        950        960 
KEHGVIGTKV CALLERLVDD YVYLFDEGGE EVIASRMYCS FSAPDEDCVA TDVVYADENQ 

       970        980        990       1000       1010       1020 
DDDADDPVVL VADTQEEDGV AREQVDSADS EICVAHTGGQ EMTEPDVVGS QTPIASAEET 

      1030       1040       1050       1060       1070       1080 
EVGEACDREG IAEVKATVCA DALDACPDQV EAFDIEKVED SILSELQTEL NAPADKTYED 

      1090       1100       1110       1120       1130       1140 
VLAFDAIYSE TLSAFYAVPS DETHFKVCGF YSPAIERTNC WLRSTLIVMQ SLPLEFKDLG 

      1150       1160       1170       1180       1190       1200 
MQKLWLSYKA GYDQCFVDKL VKSAPKSIIL PQGGYVADFA YFFLSQCSFK VHANWRCLKC 

      1210       1220       1230       1240       1250       1260 
GMELKLQGLD AVFFYGDVVS HMCKCGNSMT LLSADIPYTF DFGVRDDKFC AFYTPRKVFR 

      1270       1280       1290       1300       1310       1320 
AACAVDVNDC HSMAVVDGKQ IDGKVVTKFN GDKFDFMVGH GMTFSMSPFE IAQLYGSCIT 

      1330       1340       1350       1360       1370       1380 
PNVCFVKGDV IKVLRRVGAE VIVNPANGRM AHGAGVAGAI AKAAGKAFIN ETADMVKAQG 

      1390       1400       1410       1420       1430       1440 
VCQVGGCYES TGGKLCKKVL NIVGPDARGH GNECYSLLER AYQHINKCDN VVTTLISAGI 

      1450       1460       1470       1480       1490       1500 
FSVPTDVSLT YLLGVVTKNV ILVSNNQDDF DVIEKCQVTS VAGTKALSFQ LAKNLCRDVK 

      1510       1520       1530       1540       1550       1560 
FVTNACSSLF SESSFVSSYD VLQEVEALRH DIQLDDDARV FVQANMDCLP TDWRLVNKFD 

      1570       1580       1590       1600       1610       1620 
SVDGVRTIKY FECPGEVFVS SQGKKFGYVQ NGSFKEASVS QIRALLANKV DVLCTVDGVN 

      1630       1640       1650       1660       1670       1680 
FRSCCVAEGE VFGKTLGSVF CDGINVTKVR CSAIHKGKVF FQYSGLSAAD LAAVKDAFGF 

      1690       1700       1710       1720       1730       1740 
DEPQLLQYYS MLGMCKWPVV VCGNYFAFKQ SNNNCYINVA CLMLQHLSLK FPKWQWRRPG 

      1750       1760       1770       1780       1790       1800 
NEFRSGKPLR FVSLVLAKGS FKFNEPSDST DFIRVELREA DLSGATCDLE FICKCGVKQE 

      1810       1820       1830       1840       1850       1860 
QRKGVDAVMH FGTLDKSGLV KGYNIACTCG DKLVHCTQFN VPFLICSNTP EGKKLPDDVV 

      1870       1880       1890       1900       1910       1920 
AANIFTGGSV GHYTHVKCKP KYQLYDACNV SKVSEAKGNF TDCLYLKNLK QTFSSVLTTY 

      1930       1940       1950       1960       1970       1980 
YLDDVKCVAY KPDLSQYYCE SGKYYTKPII KAQFRTFEKV EGVYTNFKLV GHDIAEKLNA 

      1990       2000       2010       2020       2030       2040 
KLGFDCNSPF MEYKITEWPT ATGDVVLASD DLYVSRYSGG CVTFGKPVIW RGHEEASLKS 

      2050       2060       2070       2080       2090       2100 
LTYFNRPSVV CENKFNVLPV DVSEPTDRRP VPSAVLVTGA ASGADASAIS TEPGTAKEQK 

      2110       2120       2130       2140       2150       2160 
ACASDSVEDQ IVMEAQKKSS VTTVAVKEVK LNGVKKPVKW NCSVVVNDPT SETKVVKSLS 

      2170       2180       2190       2200       2210       2220 
IVDVYDMFLT GCRYVVWTAN ELSRLINSPT VREYVKWGMS KLIIPANLLL LRDEKQEFVA 

      2230       2240       2250       2260       2270       2280 
PKVVKAKAIA CYGAVKWFLL YCFSWIKFNT DNKVIYTTEV ASKLTFKLCC LAFKNALQTF 

      2290       2300       2310       2320       2330       2340 
NWSVVSRGFF LVATVFLLWF NFLYANVILS DFYLPNIGPL PMFVGQIVAW VKTTFGVLTI 

      2350       2360       2370       2380       2390       2400 
CDFYQVTDLG YRSSFCNGSM VCELCFSGFD MLDNYESINV VQHVVDRRVS FDYISLFKLV 

      2410       2420       2430       2440       2450       2460 
VELVIGYSLY TVCFYPLFVL VGMQLLTTWL PEFFMLGTMH WSARLFVFVA NMLPAFTLLR 

      2470       2480       2490       2500       2510       2520 
FYIVVTAMYK VYCLCRHVMY GCSKPGCLFC YKRNRSVRVK CSTVVGGSLR YYDVMANGGT 

      2530       2540       2550       2560       2570       2580 
GFCTKHQWNC LNCNSWKPGN TFITHEAAAD LSKELKRPVN PTDSAYYSVI EVKQVGCSMR 

      2590       2600       2610       2620       2630       2640 
LFYERDGQRV YDDVSASLFV DMNGLLHSKV KGVPETHVVV VENEADKAGF LNAAVFYAQS 

      2650       2660       2670       2680       2690       2700 
LYRPMLMVEK KLITTANTGL SVSRTMFDLY VYSLLRHLDV DRKSLTSFVN AAHNSLKEGV 

      2710       2720       2730       2740       2750       2760 
QLEQVMDTFV GCARRKCAID SDVETKSITK SVMAAVNAGV EVTDESCNNL VPTYVKSDTI 

      2770       2780       2790       2800       2810       2820 
VAADLGVLIQ NNAKHVQSNV AKAANVACIW SVDAFNQLSA DLQHRLRKAC VKTGLKIKLT 

      2830       2840       2850       2860       2870       2880 
YNKQEANVPI LTTPFSLKGG AVFSRVLQWL FVANLICFIV LWALMPTYAV HKSDMQLPLY 

      2890       2900       2910       2920       2930       2940 
ASFKVIDNGV LRDVSVTDAC FANKFNQFDQ WYESTFGLVY YRNSKACPVV VAVIDQDIGH 

      2950       2960       2970       2980       2990       3000 
TLFNVPTKVL RYGFHVLHFI THAFATDRVQ CYTPHMQIPY DNFYASGCVL SSLCTMLAHA 

      3010       3020       3030       3040       3050       3060 
DGTPHPYCYT EGVMHNASLY SSLVPHVRYN LASSNGYIRF PEVVSEGIVR VVRTRSMTYC 

      3070       3080       3090       3100       3110       3120 
RVGLCEEAEE GICFNFNSSW VLNNPYYRAM PGTFCGRNAF DLIHQVLGGL VQPIDFFALT 

      3130       3140       3150       3160       3170       3180 
ASSVAGAILA IIVVLAFYYL IKLKRAFGDY TSVVVINVIV WCINFLMLFV FQVYPTLSCL 

      3190       3200       3210       3220       3230       3240 
YACFYFYTTL YFPSEISVVM HLQWLVMYGA IMPLWFCITY VAVVVSNHAL WLFSYCRKIG 

      3250       3260       3270       3280       3290       3300 
TDVRSDGTFE EMALTTFMIT KESYCKLKNS VSDVAFNRYL SLYNKYRYFS GKMDTATYRE 

      3310       3320       3330       3340       3350       3360 
AACSQLAKAM ETFNHNNGND VLYQPPTASV TTSFLQSGIV KMVSPTSKVE PCVVSVTYGN 

      3370       3380       3390       3400       3410       3420 
MTLNGLWLDD KVYCPRHVIC SSADMTDPDY PNLLCRVTSS DFCVMSDRMS LTVMSYQMQG 

      3430       3440       3450       3460       3470       3480 
SLLVLTVTLQ NPNTPKYSFG VVKPGETFTV LAAYNGRPQG AFHVVMRSSH TIKGSFLCGS 

      3490       3500       3510       3520       3530       3540 
CGSVGYVLTG DSVRFVYMHQ LELSTGCHTG TDFSGNFYGP YRDAQVVQLP VQDYTQTVNV 

      3550       3560       3570       3580       3590       3600 
VAWLYAAILN RCNWFVQSDS CSLEEFNVWA MTNGFSSIKA DLVLDALASM TGVTVEQVLA 

      3610       3620       3630       3640       3650       3660 
AIKRLHSGFQ GKQILGSCVL EDELTPSDVY QQLAGVKLQS KRTRVIKGTC CWILASTFLF 

      3670       3680       3690       3700       3710       3720 
CSIISAFVKW TMFMYVTTHM LGVTLCALCF VIFAMLLIKH KHLYLTMYIM PVLCTLFYTN 

      3730       3740       3750       3760       3770       3780 
YLVVGYKQSF RGLAYAWLSY FVPAVDYTYM DEVLYGVVLL VAMVFVTMRS INHDVFSTMF 

      3790       3800       3810       3820       3830       3840 
LVGRLVSLVS MWYFGANLEE EVLLFLTSLF GTYTWTTMLS LATAKVIAKW LAVNVLYFTD 

      3850       3860       3870       3880       3890       3900 
IPQIKLVLLS YLCIGYVCCC YWGVLSLLNS IFRMPLGVYN YKISVQELRY MNANGLRPPR 

      3910       3920       3930       3940       3950       3960 
NSFEALMLNF KLLGIGGVPV IEVSQIQSRL TDVKCANVVL LNCLQHLHIA SNSKLWQYCS 

      3970       3980       3990       4000       4010       4020 
TLHNEILATS DLSVAFDKLA QLLVVLFANP AAVDSKCLAS IEEVSDDYVR DNTVLQALQS 

      4030       4040       4050       4060       4070       4080 
EFVNMASFVE YELAKKNLDE AKASGSANQQ QIKQLEKACN IAKSAYERDR AVARKLERMA 

      4090       4100       4110       4120       4130       4140 
DLALTNMYKE ARINDKKSKV VSALQTMLFS MVRKLDNQAL NSILDNAVKG CVPLNAIPPL 

      4150       4160       4170       4180       4190       4200 
TSNTLTIIVP DKQVFDQVVD NVYVTYAPNV WHIQSIQDAD GAVKQLNEID VNSTWPLVIS 

      4210       4220       4230       4240       4250       4260 
ANRHNEVSTV VLQNNELMPQ KLRTQVVNSG SDMNCNIPTQ CYYNTTGTGK IVYAILSDCD 

      4270       4280       4290       4300       4310       4320 
GLKYTKIVKE DGNCVVLELD PPCKFSVQDV KGLKIKYLYF VKGCNTLARG WVVGTLSSTV 

      4330       4340       4350       4360       4370       4380 
RLQAGTATEY ASNSAILSLC AFSVDPKKTY LDYIQQGGVP VTNCVKMLCD HAGTGMAITI 

      4390       4400       4410       4420       4430       4440 
KPEATTNQDS YGGASVCIYC RSRVEHPDVD GLCKLRGKFV QVPLGIKDPV SYVLTHDVCQ 

      4450       4460       4470       4480       4490       4500 
VCGFWRDGSC SCVGTGSQFQ SKDTNFLNRV RGTSVNARLV PCASGLDTDV QLRAFDICNA 

      4510       4520       4530       4540       4550       4560 
NRAGIGLYYK VNCFRFQRVD EEGNKLDKFF VVKRTNLEVY NKEKECYELT KDCGVVAEHE 

      4570       4580       4590       4600       4610       4620 
FFTFDVEGSR VPHIVRKDLS KFTMLDLCYA LRHFDRNDCS TLKEILLTYA ECDESYFQKK 

      4630       4640       4650       4660       4670       4680 
DWYDFVENPD IINVYKKLGP IFNRALLNTA NFADTLVEAG LVGVLTLDNQ DLYGQWYDFG 

      4690       4700       4710       4720       4730       4740 
DFVKTVPCCG VAVADSYYSY MMPMLTMCHA LDSELFVNGT YREFDLVQYD FTDFKLELFN 

      4750       4760       4770       4780       4790       4800 
KYFKHWSMTY HPNTSECEDD RCIIHCANFN ILFSMVLPKT CFGPLVRQIF VDGVPFVVSI 

      4810       4820       4830       4840       4850       4860 
GYHYKELGVV MNMDVDTHRY RLSLKDLLLY AADPALHVAS ASALLDLRTC CFSVAAITSG 

      4870       4880       4890       4900       4910       4920 
VKFQTVKPGN FNQDFYEFIL SKGLLKEGSS VDLKHFFFTQ DGNAAITDYN YYKYNLPTMV 

      4930       4940       4950       4960       4970       4980 
DIKQLLFVVE VVNKYFEIYE GGCIPATQVI VNNYDKSAGY PFNKFGKARL YYEALSFEEQ 

      4990       5000       5010       5020       5030       5040 
DEIYAYTKRN VLPTLTQMNL KYAISAKNRA RTVAGVSILS TMTGRMFHQK CLKSIAATRG 

      5050       5060       5070       5080       5090       5100 
VPVVIGTTKF YGGWDDMLRR LIKDVDSPVL MGWDYPKCDR AMPNILRIVS SLVLARKHDS 

      5110       5120       5130       5140       5150       5160 
CCSHTDRFYR LANECAQVLG EIVMCGGCYY VKPGGTSSGD ATTAFANSVF NICQAVSANV 

      5170       5180       5190       5200       5210       5220 
CSLMACNGHK IEDLSIRELQ KRLYSNVYRA DHVDPAFVSE YYEFLNKHFS MIILSDDGVV 

      5230       5240       5250       5260       5270       5280 
CYNSEFASKG YIANISDFQQ VLYYQNNVFM SEAKCWVETD IEKGPHEFCS QHTMLVKMDG 

      5290       5300       5310       5320       5330       5340 
DEVYLPYPDP SRILGAGCFV DDLLKTDSVL LIERFVSLAI DAYPLVYHEN PEYQNVFRVY 

      5350       5360       5370       5380       5390       5400 
LEYIKKLYND LGNQILDSIS VILSTCDGQK FTDETFYKNM YLRSAVMQSV GACVVCSSQT 

      5410       5420       5430       5440       5450       5460 
SLRCGSCIRK PLLCCKCAYD HVMSTDHKYV LSVSPYVCNS PGCDVNDVTK LYLGGMSYYC 

      5470       5480       5490       5500       5510       5520 
EAHKPQYSFK LVMNGMVFGL YKQSCTGSPY IEDFNKIASC KWTEVDDYVL ANECTERLKL 

      5530       5540       5550       5560       5570       5580 
FAAETQKATE EAFKQCYASA TIREIVSDRE LILSWEIGKV RPPLNKNYVF TGYHFTNNGK 

      5590       5600       5610       5620       5630       5640 
TVLGEYVFDK SELTNGVYYR ATTTYKLSVG DVFILTSHAV SSLSAPTLVP QENYTSVRFA 

      5650       5660       5670       5680       5690       5700 
SAYSVPETFQ NNVPNYQHIG IKRYCTVQGP PGTGKSHLAI GHAVYYCTAR VVYTAASHAA 

      5710       5720       5730       5740       5750       5760 
VDALCEKAHK FLNINDCARI VPAKLRVDCY DKFNVNDTTR KYVFTTINAL PELVTDIIVV 

      5770       5780       5790       5800       5810       5820 
DEVSMLTNYE LSVINSRVRA KHYVYIGDPA QLPAPRVLLN KGTLEPRYFN SVTKLMCCLG 

      5830       5840       5850       5860       5870       5880 
PDIFLGTCYR CPKEIVDTVS ALVYNNKLKA KNDNSAMCFK VYYKGQTTHE SSSAVNMQQI 

      5890       5900       5910       5920       5930       5940 
HLISKLLKAN PSWSNAVFIS PYNSQNYVAK RVLGLQTQTA DSAQGSAYDF VIYSQTAQTA 

      5950       5960       5970       5980       5990       6000 
HSVNVNRFNV AITRAKKGIL CVMSSMQLIG VFNFTTLTLD KINNPRLQCT TNLFKDCSKS 

      6010       6020       6030       6040       6050       6060 
YVGIPPCAFL LAVDDKYKVS GNLAVCLNVA DSAVTYSRLI SLMGFKLDLT LDGYCKLFIT 

      6070       6080       6090       6100       6110       6120 
RDEAIKRVRA WVGFDAEGAH ATRDSIGTNF PLQLGFSTGI DFVVEATGMF AERDGYVFKK 

      6130       6140       6150       6160       6170       6180 
AAARAPPGEQ FKHLVPLMSR GQKWDVVRIR IVQMLSDHLV DLADSVVLVT WAASFELTCL 

      6190       6200       6210       6220       6230       6240 
RYFAKVGKEV VCSVCNKRAT CFNSRTGYYG CWRHSYSCDY LYNPLIVDIQ QWGYTGSLTS 

      6250       6260       6270       6280       6290       6300 
NHDPICSVHK GAHVASSDAI MTRCLAVHDC FCKSVNWNLE YPIISNEVSV NTSCRLLQRV 

      6310       6320       6330       6340       6350       6360 
MFRAAMLCNR YDVCYDIGNP KGLACVKGYD FKFYDASPVV KSVKQFVYKY EAHKDQFLDG 

      6370       6380       6390       6400       6410       6420 
LCMFWNCNVD KYPANAVVCR FDTRVLSKLN LPGCNGGSLY VNKHAFHTNP FTRAAFENLK 

      6430       6440       6450       6460       6470       6480 
PMPFFYYSDT PCVYMEGMES KQVDYVPLRS ATCITRCNLG GAVCLKHAEE YREYLESYNT 

      6490       6500       6510       6520       6530       6540 
ATTAGFTFWV YKTFDFYNLW NTFTRLQSLE NVVYNLVNAG HFDGRAGELP CAVIGEKVIA 

      6550       6560       6570       6580       6590       6600 
KIQNEDVVVF KNNTPFPTNV AVELFAERSI RPHPELKLFR SSNIHVCWNH VLWDYAKDSV 

      6610       6620       6630       6640       6650       6660 
FCSSTYKVCK YTDLQCIESL NVLFDGRDNG ALEAFKKCRN GVYINTTKIK SLSMIKGPQR 

      6670       6680       6690       6700       6710       6720 
ADLNGVVVEK VGDSDVEFWF AMRRDGDDVI FSRTGSLEPS HYRSPQGNPG GNRVGDLSGN 

      6730       6740       6750       6760       6770       6780 
EALARGTIFT QSRFLSSFSP RSEMEKDFMD LDEDVFIAKY SLQDYAFEHV VYGSFNQKII 

      6790       6800       6810       6820       6830       6840 
GGLHLLIGLA RRPKKSNLVI QEFVPYDSSI HSYFITDENS GSSESVCTVI DLLLDDFVDI 

      6850       6860       6870       6880       6890       6900 
VKSLNLKCVS KVVNVNVDFK DFQFMLWCNE EKVMTFYPRL QAAADWKPGY VMPVLYKYLE 

      6910       6920       6930       6940       6950       6960 
SPMERVNLWN YGKPITLPTG CMMNVAKYTQ LCQYLSTTTL AVPANMRVLH LGAGSDKGVA 

      6970       6980       6990       7000       7010       7020 
PGSAVLRQWL PSGSILVDND MNPFVSDSVA SYYGNCITLP FDCQWDLIIS DMYDPLTKNI 

      7030       7040       7050       7060       7070       7080 
GEYNVSKDGF FTYLCHLIRD KLALGGSVAI KITEFSWNAE LYSLMGKFAF WTIFCTNVNA 

      7090       7100       7110       7120       7130       7140 
SSSEGFLIGI NWLNRTRNEI DGKTMHANYL FWRNSTMWNG GAYSLFDMTK FPLKAAGTAV 

      7150       7160       7170       7180 
VSLKPDQIND LVLSLIEKGK LLVRDTRKEV FVGDSLVNVK 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6V1.

References

[1]"The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase."
Lee H.-J., Shieh C.-K., Gorbalenya A.E., Koonin E.V., la Monica N., Tuler J., Bagdzhardzhyan A., Lai M.M.C.
Virology 180:567-582(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Sequence and translation of the murine coronavirus 5'-end genomic RNA reveals the N-terminal structure of the putative RNA polymerase."
Soe L.H., Shieh C.-K., Baker S.C., Chang M.F., Lai M.M.C.
J. Virol. 61:3968-3976(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-595.
[3]"Mouse hepatitis virus strain A59 RNA polymerase gene ORF 1a: heterogeneity among MHV strains."
Bonilla P.J., Gorbalenya A.E., Weiss S.R.
Virology 198:736-740(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"Murine coronavirus gene 1 polyprotein contains an autoproteolytic activity."
Baker S.C., La Monica N., Shieh C.K., Lai M.M.
Adv. Exp. Med. Biol. 276:283-289(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1021-1326.
[5]"Identification of the murine coronavirus MP1 cleavage site recognized by papain-like proteinase 2."
Kanjanahaluethai A., Jukneliene D., Baker S.C.
J. Virol. 77:7376-7382(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, MUTAGENESIS OF PHE-2835; SER-2836; LEU-2837; LYS-2838; GLY-2839; GLY-2840; ALA-2841; VAL-2842 AND VAL-2846.
[6]"Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a."
Schiller J.J., Kanjanahaluethai A., Baker S.C.
Virology 242:288-302(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, SUBCELLULAR LOCATION.
[7]"Conservation of substrate specificities among coronavirus main proteases."
Hegyi A., Ziebuhr J.
J. Gen. Virol. 83:595-599(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55148 Genomic RNA. Translation: AAA46457.1. Sequence problems.
M55148 Genomic RNA. Translation: AAA46458.2. Sequence problems.
M18040 Genomic RNA. Translation: AAA46466.1.
S51684 Genomic RNA. Translation: AAB19566.1.
PIRRRIHM2. A36815.
VFIHJH. B36815.
RefSeqYP_209229.2. AC_000192.1.

3D structure databases

ProteinModelPortalP0C6Y0.
SMRP0C6Y0. Positions 4055-4205, 4329-4453, 6508-6876.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3283258.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR022570. Coronavirus_NSP1.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR002705. Pept_C30/C16_sub.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF11963. DUF3477. 2 hits.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_CVMJH
AccessionPrimary (citable) accession number: P0C6Y0
Secondary accession number(s): P19751 expand/collapse secondary AC list , P29982, Q66194, Q90045
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries