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Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Replicase polyprotein 1ab: Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.
Host translation inhibitor nsp1: Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.1 Publication
Non-structural protein 2: May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.1 Publication
Papain-like proteinase: Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.2 Publications
Non-structural protein 4: Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.1 Publication
Proteinase 3CL-PRO: Ccleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).PROSITE-ProRule annotation1 Publication
Non-structural protein 6: Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.1 Publication
Non-structural protein 7: Forms an hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.1 Publication
Non-structural protein 8: Forms an hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.1 Publication
Non-structural protein 9: May participate in viral replication by acting as a ssRNA-binding protein.1 Publication
Non-structural protein 10: Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.1 Publication
RNA-directed RNA polymerase: Responsible for replication and transcription of the viral RNA genome.1 Publication
Helicase: Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.2 Publications
Guanine-N7 methyltransferase: Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.3 Publications
Uridylate-specific endoribonuclease: Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
2'-O-methyltransferase: Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.Curated2 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation1 Publication
ATP + H2O = ADP + phosphate.1 Publication
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.1 Publication
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Kineticsi

The kinetic parameters are studied for the 3C-like proteinase domain. The cleavage takes place at the /.

  1. KM=1.15 mM for peptide TSAVLQ/SGFRK-NH21 Publication
  2. KM=0.58 mM for peptide SGVTFQ/GKFKK1 Publication
  3. KM=1.44 mM for peptide ATVRLQ/AGNAT1 Publication

    pH dependencei

    Optimum pH is 7.0 for 3C-like proteinase activity.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei180 – 1812CleavageBy similarity
    Sitei818 – 8192Cleavage; by PL-PROBy similarity
    Active sitei1651 – 16511For PL-PRO activityPROSITE-ProRule annotation
    Active sitei1812 – 18121For PL-PRO activityPROSITE-ProRule annotation
    Sitei2740 – 27412Cleavage; by PL-PROBy similarity
    Sitei3240 – 32412Cleavage; by 3CL-PROBy similarity
    Active sitei3281 – 32811For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3385 – 33851For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3546 – 35472Cleavage; by 3CL-PROBy similarity
    Sitei3836 – 38372Cleavage; by 3CL-PROBy similarity
    Sitei3919 – 39202Cleavage; by 3CL-PROBy similarity
    Sitei4117 – 41182Cleavage; by 3CL-PROBy similarity
    Sitei4230 – 42312Cleavage; by 3CL-PROBy similarity
    Metal bindingi4304 – 43041Zinc
    Metal bindingi4307 – 43071Zinc
    Metal bindingi4313 – 43131Zinc
    Metal bindingi4320 – 43201Zinc
    Metal bindingi4347 – 43471Zinc
    Metal bindingi4350 – 43501Zinc
    Metal bindingi4358 – 43581Zinc
    Sitei4369 – 43702Cleavage; by 3CL-PROBy similarity
    Sitei5301 – 53022Cleavage; by 3CL-PROBy similarity
    Sitei5902 – 59032Cleavage; by 3CL-PROBy similarity
    Sitei6429 – 64302Cleavage; by 3CL-PROBy similarity
    Sitei6775 – 67762Cleavage; by 3CL-PROBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1729 – 176638C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4304 – 432017Add
    BLAST
    Zinc fingeri4347 – 436014Add
    BLAST
    Nucleotide bindingi5583 – 55908ATPBy similarity

    GO - Molecular functioni

    • 3'-5'-exoribonuclease activity Source: CACAO
    • ATP binding Source: UniProtKB-KW
    • cysteine-type endopeptidase activity Source: InterPro
    • endonuclease activity Source: UniProtKB-KW
    • helicase activity Source: UniProtKB
    • Lys48-specific deubiquitinase activity Source: UniProtKB
    • methyltransferase activity Source: InterPro
    • mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
    • mRNA (nucleoside-2'-O-)-methyltransferase activity Source: UniProtKB
    • omega peptidase activity Source: InterPro
    • RNA binding Source: UniProtKB-KW
    • RNA-directed RNA polymerase activity Source: UniProtKB
    • single-stranded RNA binding Source: UniProtKB
    • zinc ion binding Source: InterPro

    GO - Biological processi

    • 7-methylguanosine mRNA capping Source: UniProtKB
    • induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    • induction by virus of host autophagy Source: UniProtKB-KW
    • metabolic process Source: GOC
    • modulation by virus of host autophagy Source: UniProtKB
    • modulation by virus of host protein ubiquitination Source: UniProtKB
    • mRNA methylation Source: UniProtKB
    • positive regulation of ubiquitin-specific protease activity Source: UniProtKB
    • protein K48-linked deubiquitination Source: GOC
    • RNA (guanine-N7)-methylation Source: GOC
    • RNA phosphodiester bond hydrolysis, exonucleolytic Source: UniProtKB
    • suppression by virus of host IRF3 activity by inhibition of IRF3 phosphorylation Source: UniProtKB
    • suppression by virus of host ISG15 activity Source: UniProtKB
    • suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB
    • suppression by virus of host translation Source: UniProtKB
    • suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB
    • transcription, DNA-templated Source: InterPro
    • transcription, RNA-templated Source: UniProtKB
    • viral protein processing Source: InterPro
    • viral RNA genome replication Source: InterPro
    • viral transcription Source: CACAO
    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Inhibition of host NF-kappa-B by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.7.48. 7599.
    3.4.22.B14. 7599.
    3.6.4.12. 7599.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Short name:
    pp1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 15 chains:
    Alternative name(s):
    Leader protein
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p65 homolog
    Papain-like proteinase (EC:3.4.19.12, EC:3.4.22.69)
    Short name:
    PL-PRO
    Alternative name(s):
    Non-structural protein 3
    Short name:
    nsp3
    PL2-PRO
    SARS coronavirus main proteinase
    Non-structural protein 4
    Short name:
    nsp4
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    nsp5
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Non-structural protein 8
    Short name:
    nsp8
    Non-structural protein 9
    Short name:
    nsp9
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    nsp12
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    nsp13
    Alternative name(s):
    nsp14
    Alternative name(s):
    NendoU
    nsp15
    Alternative name(s):
    nsp16
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
    Taxonomic identifieri227859 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Paguma larvata (Masked palm civet) [TaxID: 9675]
    ProteomesiUP000000354 Componenti: Genome

    Subcellular locationi

    Chain Papain-like proteinase :
    Chain Non-structural protein 4 :
    Chain Non-structural protein 7 :
    • Host cytoplasmhost perinuclear region By similarity

    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
    Chain Non-structural protein 8 :
    • Host cytoplasmhost perinuclear region By similarity

    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
    Chain Non-structural protein 9 :
    • Host cytoplasmhost perinuclear region By similarity

    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
    Chain Non-structural protein 10 :
    • Host cytoplasmhost perinuclear region By similarity

    • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
    Chain Helicase :

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2092 – 211221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2203 – 222321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2304 – 232421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2326 – 234621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2351 – 237121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2755 – 277521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2830 – 285021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2879 – 289921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2992 – 301221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3022 – 304221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3054 – 307421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3077 – 309721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3105 – 312521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3142 – 316221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3564 – 358421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3586 – 360621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3612 – 363221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3658 – 367821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3707 – 372721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3728 – 374821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3756 – 377621HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi4217 – 42171G → E: Complete loss of nsp9 dimerization. 1 Publication
    Mutagenesisi4221 – 42211G → E: Complete loss of nsp9 dimerization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 180180Host translation inhibitor nsp1By similarityPRO_0000037309Add
    BLAST
    Chaini181 – 818638Non-structural protein 2By similarityPRO_0000037310Add
    BLAST
    Chaini819 – 27401922Papain-like proteinaseBy similarityPRO_0000037311Add
    BLAST
    Chaini2741 – 3240500Non-structural protein 4Sequence AnalysisPRO_0000283841Add
    BLAST
    Chaini3241 – 35463063C-like proteinaseBy similarityPRO_0000037312Add
    BLAST
    Chaini3547 – 3836290Non-structural protein 6By similarityPRO_0000037313Add
    BLAST
    Chaini3837 – 391983Non-structural protein 7By similarityPRO_0000037314Add
    BLAST
    Chaini3920 – 4117198Non-structural protein 8By similarityPRO_0000037315Add
    BLAST
    Chaini4118 – 4230113Non-structural protein 9By similarityPRO_0000037316Add
    BLAST
    Chaini4231 – 4369139Non-structural protein 10By similarityPRO_0000037317Add
    BLAST
    Chaini4370 – 5301932RNA-directed RNA polymeraseBy similarityPRO_0000037318Add
    BLAST
    Chaini5302 – 5902601HelicaseBy similarityPRO_0000037319Add
    BLAST
    Chaini5903 – 6429527Guanine-N7 methyltransferaseBy similarityPRO_0000037320Add
    BLAST
    Chaini6430 – 6775346Uridylate-specific endoribonucleaseBy similarityPRO_0000037321Add
    BLAST
    Chaini6776 – 70732982'-O-methyltransferaseBy similarityPRO_0000037322Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

    Proteomic databases

    PRIDEiP0C6X7.

    Interactioni

    Subunit structurei

    Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.8 Publications

    Protein-protein interaction databases

    MINTiMINT-1487760.

    Structurei

    Secondary structure

    1
    7073
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi14 – 207Combined sources
    Turni23 – 253Combined sources
    Helixi35 – 4814Combined sources
    Beta strandi51 – 544Combined sources
    Helixi61 – 633Combined sources
    Beta strandi68 – 725Combined sources
    Beta strandi87 – 937Combined sources
    Turni95 – 984Combined sources
    Beta strandi99 – 1024Combined sources
    Beta strandi105 – 1095Combined sources
    Beta strandi116 – 1238Combined sources
    Beta strandi827 – 8293Combined sources
    Turni831 – 8333Combined sources
    Beta strandi839 – 8413Combined sources
    Beta strandi848 – 8514Combined sources
    Turni852 – 8565Combined sources
    Beta strandi860 – 8623Combined sources
    Turni869 – 8713Combined sources
    Helixi872 – 88211Combined sources
    Helixi888 – 8947Combined sources
    Helixi898 – 9014Combined sources
    Beta strandi907 – 9093Combined sources
    Beta strandi911 – 9155Combined sources
    Beta strandi918 – 9203Combined sources
    Beta strandi922 – 9265Combined sources
    Beta strandi1013 – 10219Combined sources
    Helixi1023 – 10308Combined sources
    Beta strandi1033 – 10386Combined sources
    Helixi1048 – 10569Combined sources
    Turni1057 – 10593Combined sources
    Helixi1060 – 107213Combined sources
    Beta strandi1080 – 10845Combined sources
    Turni1086 – 10883Combined sources
    Beta strandi1090 – 10956Combined sources
    Helixi1100 – 11023Combined sources
    Helixi1108 – 11147Combined sources
    Helixi1115 – 11184Combined sources
    Beta strandi1119 – 11246Combined sources
    Helixi1130 – 11323Combined sources
    Helixi1136 – 114611Combined sources
    Beta strandi1149 – 11568Combined sources
    Helixi1158 – 116811Combined sources
    Beta strandi1345 – 13473Combined sources
    Helixi1351 – 136111Combined sources
    Beta strandi1364 – 13685Combined sources
    Helixi1372 – 138110Combined sources
    Beta strandi1389 – 140113Combined sources
    Helixi1407 – 141711Combined sources
    Beta strandi1421 – 14244Combined sources
    Beta strandi1426 – 14283Combined sources
    Helixi1429 – 14313Combined sources
    Helixi1435 – 14428Combined sources
    Beta strandi1449 – 14524Combined sources
    Helixi1457 – 146711Combined sources
    Beta strandi1544 – 155512Combined sources
    Beta strandi1557 – 15626Combined sources
    Helixi1567 – 15715Combined sources
    Beta strandi1572 – 15765Combined sources
    Helixi1588 – 15903Combined sources
    Beta strandi1594 – 15974Combined sources
    Helixi1602 – 161211Combined sources
    Helixi1619 – 163012Combined sources
    Helixi1651 – 166111Combined sources
    Beta strandi1667 – 16693Combined sources
    Helixi1670 – 168011Combined sources
    Helixi1685 – 169410Combined sources
    Helixi1705 – 17139Combined sources
    Beta strandi1722 – 17287Combined sources
    Beta strandi1730 – 17323Combined sources
    Beta strandi1734 – 17407Combined sources
    Helixi1742 – 17454Combined sources
    Beta strandi1746 – 17494Combined sources
    Helixi1753 – 17586Combined sources
    Beta strandi1760 – 17634Combined sources
    Beta strandi1765 – 178521Combined sources
    Beta strandi1790 – 17934Combined sources
    Beta strandi1799 – 18079Combined sources
    Beta strandi1810 – 182617Combined sources
    Beta strandi1829 – 18346Combined sources
    Beta strandi1836 – 184611Combined sources
    Beta strandi1904 – 19118Combined sources
    Helixi1912 – 192110Combined sources
    Beta strandi1929 – 19368Combined sources
    Beta strandi1942 – 19487Combined sources
    Helixi1949 – 19513Combined sources
    Helixi1954 – 19563Combined sources
    Beta strandi1963 – 19664Combined sources
    Beta strandi1969 – 19713Combined sources
    Helixi1977 – 199014Combined sources
    Helixi3251 – 32544Combined sources
    Beta strandi3257 – 32626Combined sources
    Beta strandi3265 – 32728Combined sources
    Beta strandi3275 – 32795Combined sources
    Helixi3280 – 32834Combined sources
    Helixi3288 – 32903Combined sources
    Helixi3294 – 32996Combined sources
    Helixi3303 – 33053Combined sources
    Beta strandi3306 – 33105Combined sources
    Beta strandi3313 – 33153Combined sources
    Beta strandi3317 – 33237Combined sources
    Beta strandi3326 – 33338Combined sources
    Beta strandi3340 – 33434Combined sources
    Beta strandi3351 – 33588Combined sources
    Beta strandi3361 – 33699Combined sources
    Helixi3379 – 33813Combined sources
    Beta strandi3388 – 33936Combined sources
    Beta strandi3396 – 340611Combined sources
    Turni3408 – 34103Combined sources
    Beta strandi3412 – 34154Combined sources
    Beta strandi3421 – 34244Combined sources
    Beta strandi3427 – 34304Combined sources
    Helixi3441 – 345313Combined sources
    Turni3458 – 34603Combined sources
    Helixi3467 – 347610Combined sources
    Beta strandi3477 – 34793Combined sources
    Helixi3484 – 34896Combined sources
    Helixi3491 – 34977Combined sources
    Helixi3501 – 351414Combined sources
    Beta strandi3516 – 35183Combined sources
    Beta strandi3521 – 35233Combined sources
    Beta strandi3524 – 35263Combined sources
    Helixi3533 – 35397Combined sources
    Turni3540 – 35423Combined sources
    Helixi3837 – 385519Combined sources
    Helixi3862 – 387615Combined sources
    Beta strandi3878 – 38803Combined sources
    Helixi3881 – 390525Combined sources
    Helixi3906 – 39094Combined sources
    Turni3910 – 39123Combined sources
    Helixi3913 – 39164Combined sources
    Turni3959 – 39635Combined sources
    Turni3970 – 39723Combined sources
    Helixi3973 – 398715Combined sources
    Helixi3996 – 401722Combined sources
    Helixi4020 – 403011Combined sources
    Beta strandi4034 – 40374Combined sources
    Beta strandi4046 – 40516Combined sources
    Helixi4054 – 40607Combined sources
    Beta strandi4065 – 40684Combined sources
    Beta strandi4071 – 40799Combined sources
    Helixi4088 – 40903Combined sources
    Turni4093 – 40953Combined sources
    Helixi4096 – 40983Combined sources
    Beta strandi4103 – 41097Combined sources
    Beta strandi4120 – 41256Combined sources
    Beta strandi4127 – 41315Combined sources
    Beta strandi4134 – 41374Combined sources
    Beta strandi4145 – 41506Combined sources
    Beta strandi4153 – 41553Combined sources
    Beta strandi4157 – 41648Combined sources
    Beta strandi4170 – 41745Combined sources
    Beta strandi4181 – 41866Combined sources
    Beta strandi4191 – 41944Combined sources
    Beta strandi4201 – 42088Combined sources
    Helixi4213 – 422210Combined sources
    Helixi4224 – 42274Combined sources
    Helixi4243 – 42486Combined sources
    Beta strandi4250 – 42523Combined sources
    Helixi4253 – 426210Combined sources
    Beta strandi4284 – 42885Combined sources
    Beta strandi4295 – 43006Combined sources
    Helixi4301 – 43033Combined sources
    Helixi4305 – 43084Combined sources
    Beta strandi4314 – 43185Combined sources
    Beta strandi4325 – 43306Combined sources
    Helixi4331 – 43333Combined sources
    Helixi4337 – 43437Combined sources
    Turni4348 – 43503Combined sources
    Turni4354 – 43574Combined sources
    Helixi6431 – 644111Combined sources
    Beta strandi6453 – 64564Combined sources
    Beta strandi6459 – 64646Combined sources
    Beta strandi6467 – 64737Combined sources
    Beta strandi6476 – 64783Combined sources
    Helixi6480 – 64889Combined sources
    Beta strandi6493 – 64953Combined sources
    Helixi6498 – 65036Combined sources
    Beta strandi6508 – 65136Combined sources
    Turni6517 – 65204Combined sources
    Beta strandi6521 – 653010Combined sources
    Turni6532 – 65343Combined sources
    Beta strandi6535 – 65395Combined sources
    Helixi6543 – 65453Combined sources
    Beta strandi6550 – 65534Combined sources
    Helixi6559 – 65657Combined sources
    Beta strandi6567 – 65759Combined sources
    Beta strandi6589 – 65913Combined sources
    Beta strandi6594 – 65963Combined sources
    Beta strandi6599 – 66013Combined sources
    Beta strandi6606 – 66116Combined sources
    Beta strandi6614 – 66163Combined sources
    Helixi6629 – 66313Combined sources
    Helixi6637 – 66448Combined sources
    Helixi6647 – 66537Combined sources
    Helixi6661 – 66644Combined sources
    Beta strandi6670 – 66734Combined sources
    Beta strandi6677 – 66793Combined sources
    Helixi6680 – 668910Combined sources
    Beta strandi6692 – 66987Combined sources
    Beta strandi6703 – 67119Combined sources
    Turni6712 – 67143Combined sources
    Beta strandi6717 – 67248Combined sources
    Helixi6728 – 67369Combined sources
    Beta strandi6741 – 675111Combined sources
    Beta strandi6754 – 676310Combined sources
    Beta strandi6766 – 67727Combined sources
    Helixi6777 – 67804Combined sources
    Beta strandi6781 – 67855Combined sources
    Helixi6788 – 67914Combined sources
    Helixi6817 – 682913Combined sources
    Beta strandi6841 – 68466Combined sources
    Helixi6855 – 68639Combined sources
    Beta strandi6869 – 68768Combined sources
    Beta strandi6881 – 68888Combined sources
    Helixi6890 – 68923Combined sources
    Beta strandi6893 – 68975Combined sources
    Beta strandi6899 – 69046Combined sources
    Helixi6924 – 693512Combined sources
    Beta strandi6936 – 694611Combined sources
    Beta strandi6948 – 69503Combined sources
    Helixi6953 – 69597Combined sources
    Beta strandi6962 – 69709Combined sources
    Helixi6971 – 69733Combined sources
    Beta strandi6979 – 69868Combined sources
    Helixi6996 – 700914Combined sources
    Helixi7017 – 70204Combined sources
    Beta strandi7033 – 70353Combined sources
    Helixi7039 – 70413Combined sources
    Helixi7044 – 70518Combined sources
    Beta strandi7055 – 70573Combined sources
    Beta strandi7065 – 70673Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1O5Smodel-A4745-5301[»]
    1P76model-A3241-3541[»]
    B4225-4231[»]
    1P9Tmodel-A3241-3544[»]
    1PA5model-A3241-3546[»]
    1PUKmodel-A3241-3550[»]
    1Q1Xmodel-A3241-3542[»]
    1Q2WX-ray1.86A/B3241-3544[»]
    1QZ8X-ray2.70A/B4118-4230[»]
    1SXFmodel-A4765-5244[»]
    1UJ1X-ray1.90A/B3241-3546[»]
    1UK2X-ray2.20A/B3241-3546[»]
    1UK3X-ray2.40A/B3241-3546[»]
    1UK4X-ray2.50A/B3241-3546[»]
    1UW7X-ray2.80A4118-4230[»]
    1WOFX-ray2.00A/B3241-3546[»]
    1YSYNMR-A3837-3919[»]
    1Z1IX-ray2.80A3241-3546[»]
    1Z1JX-ray2.80A/B3241-3546[»]
    2A5AX-ray2.08A3241-3546[»]
    2A5IX-ray1.88A3241-3546[»]
    2A5KX-ray2.30A/B3241-3546[»]
    2ACFX-ray1.40A/B/C/D1002-1176[»]
    2AHMX-ray2.40A/B/C/D3837-3919[»]
    E/F/G/H3920-4117[»]
    2AJ5model-A3241-3546[»]
    2ALVX-ray1.90A3241-3543[»]
    2AMDX-ray1.85A/B3241-3546[»]
    2AMQX-ray2.30A/B3241-3546[»]
    2BX3X-ray2.00A3241-3546[»]
    2BX4X-ray2.79A3241-3546[»]
    2C3SX-ray1.90A3241-3546[»]
    2D2DX-ray2.70A/B3241-3546[»]
    2DUCX-ray1.70A/B3241-3546[»]
    2FAVX-ray1.80A/B/C1000-1173[»]
    2FE8X-ray1.85A/B/C1541-1854[»]
    2FYGX-ray1.80A4240-4362[»]
    2G1Fmodel-A/B5302-5877[»]
    2G9TX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X4231-4378[»]
    2GA6X-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X4231-4378[»]
    2GDTNMR-A13-127[»]
    2GRINMR-A819-930[»]
    2GT7X-ray1.82A/B3241-3546[»]
    2GT8X-ray2.00A3241-3546[»]
    2GTBX-ray2.00A3241-3546[»]
    2GX4X-ray1.93A3241-3546[»]
    2GZ7X-ray1.86A3241-3546[»]
    2GZ8X-ray1.97A3241-3546[»]
    2GZ9X-ray2.17A3241-3546[»]
    2H2ZX-ray1.60A3241-3546[»]
    2H85X-ray2.60A6429-6774[»]
    2HOBX-ray1.95A3241-3546[»]
    2HSXNMR-A13-127[»]
    2IDYNMR-A819-930[»]
    2JZDNMR-A1345-1469[»]
    2JZENMR-A1345-1469[»]
    2JZFNMR-A1331-1469[»]
    2K87NMR-A1884-1998[»]
    2OP9X-ray1.80A/B3241-3541[»]
    2OZKX-ray2.90A/B/C/D6430-6775[»]
    2PWXX-ray2.50A3241-3546[»]
    2Q6GX-ray2.50A/B3241-3546[»]
    2QC2X-ray2.70A/B3241-3546[»]
    2QCYX-ray1.75A3241-3546[»]
    2QIQX-ray1.90A3242-3541[»]
    2RHBX-ray2.80A/B/C/D/E/F6430-6775[»]
    2RNKNMR-A1331-1469[»]
    2V6NX-ray1.98A3241-3546[»]
    2VJ1X-ray2.25A/B3242-3544[»]
    2XYQX-ray2.00A6776-7065[»]
    B4240-4361[»]
    2XYRX-ray2.50A6776-7067[»]
    B4240-4361[»]
    2XYVX-ray2.06A6776-7067[»]
    B4240-4361[»]
    2YY4X-ray2.20A/B3241-3556[»]
    2Z3CX-ray1.79A3241-3546[»]
    2Z3DX-ray2.10A3241-3546[»]
    2Z3EX-ray2.32A3241-3546[»]
    2Z94X-ray1.78A3241-3546[»]
    2Z9GX-ray1.86A3241-3546[»]
    2Z9JX-ray1.95A/B3241-3546[»]
    2Z9KX-ray1.85A/B3241-3546[»]
    2Z9LX-ray2.10A/B3241-3546[»]
    3D62X-ray2.70A3243-3541[»]
    3E9SX-ray2.50A1541-1855[»]
    3EBNX-ray2.40A/B/C/D3429-3546[»]
    3R24X-ray2.00A6776-7073[»]
    B4240-4382[»]
    4TWWX-ray2.42A/B3241-3546[»]
    4TWYX-ray1.60A3241-3546[»]
    4WY3X-ray1.89A3241-3546[»]
    ProteinModelPortaliP0C6X7.
    SMRiP0C6X7. Positions 13-127, 819-930, 1002-1176, 1331-1469, 1541-1854, 3241-3546, 3837-3910, 3921-4111, 4118-4230, 4240-4362, 6430-6773.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C6X7.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1003 – 1169167MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1611 – 1875265Peptidase C16PROSITE-ProRule annotationAdd
    BLAST
    Domaini3241 – 3546306Peptidase C30PROSITE-ProRule annotationAdd
    BLAST
    Domaini4981 – 5143163RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini5302 – 538584CV MBDAdd
    BLAST
    Domaini5558 – 5739182(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini5740 – 5909170(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2092 – 2371280HD1Add
    BLAST
    Regioni2755 – 3162408HD2Add
    BLAST
    Regioni3564 – 3776213HD3Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi930 – 100172Glu-richAdd
    BLAST
    Compositional biasi2210 – 22134Poly-Leu
    Compositional biasi3766 – 37694Poly-Cys

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1729 – 176638C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4304 – 432017Add
    BLAST
    Zinc fingeri4347 – 436014Add
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

    Isoform Replicase polyprotein 1ab (identifier: P0C6X7-1) [UniParc]FASTAAdd to basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MESLVLGVNE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKNGT
    60 70 80 90 100
    CGLVELEKGV LPQLEQPYVF IKRSDALSTN HGHKVVELVA EMDGIQYGRS
    110 120 130 140 150
    GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSYGIDL KSYDLGDELG
    160 170 180 190 200
    TDPIEDYEQN WNTKHGSGAL RELTRELNGG AVTRYVDNNF CGPDGYPLDC
    210 220 230 240 250
    IKDFLARAGK SMCTLSEQLD YIESKRGVYC CRDHEHEIAW FTERSDKSYE
    260 270 280 290 300
    HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI
    310 320 330 340 350
    RSVYPVASPQ ECNNMHLSTL MKCNHCDEVS WQTCDFLKAT CEHCGTENLV
    360 370 380 390 400
    IEGPTTCGYL PTNAVVKMPC PACQDPEIGP EHSVADYHNH SNIETRLRKG
    410 420 430 440 450
    GRTRCFGGCV FAYVGCYNKR AYWVPRASAD IGSGHTGITG DNVETLNEDL
    460 470 480 490 500
    LEILSRERVN INIVGDFHLN EEVAIILASF SASTSAFIDT IKSLDYKSFK
    510 520 530 540 550
    TIVESCGNYK VTKGKPVKGA WNIGQQRSVL TPLCGFPSQA AGVIRSIFAR
    560 570 580 590 600
    TLDAANHSIP DLQRAAVTIL DGISEQSLRL VDAMVYTSDL LTNSVIIMAY
    610 620 630 640 650
    VTGGLVQQTS QWLSNLLGTT VEKLRPIFEW IEAKLSAGVE FLKDAWEILK
    660 670 680 690 700
    FLITGVFDIV KGQIQVASDN IKDCVKCFID VVNKALEMCI DQVTIAGAKL
    710 720 730 740 750
    RSLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDSHDTV
    760 770 780 790 800
    LTSEEVVLKN GELEALETPV DSFTNGAIVG TPVCVNGLML LEIKDKEQYC
    810 820 830 840 850
    ALSPGLLATN NVFRLKGGAP IKGVTFGEDT VWEVQGYKNV RITFELDERV
    860 870 880 890 900
    DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE
    910 920 930 940 950
    WSVATFYLFD DAGEENFSSR MYCSFYPPDE EEEDDAECEE EEIDETCEHE
    960 970 980 990 1000
    YGTEDDYQGL PLEFGASAET VRVEEEEEED WLDDTTEQSE IEPEPEPTPE
    1010 1020 1030 1040 1050
    EPVNQFTGYL KLTDNVAIKC VDIVKEAQSA NPMVIVNAAN IHLKHGGGVA
    1060 1070 1080 1090 1100
    GALNKATNGA MQKESDDYIK LNGPLTVGGS CLLSGHNLAK KCLHVVGPNL
    1110 1120 1130 1140 1150
    NAGEDIQLLK AAYENFNSQD ILLAPLLSAG IFGAKPLQSL QVCVQTVRTQ
    1160 1170 1180 1190 1200
    VYIAVNDKAL YEQVVMDYLD NLKPRVEAPK QEEPPNTEDS KTEEKSVVQK
    1210 1220 1230 1240 1250
    PVDVKPKIKA CIDEVTTTLE ETKFLTNKLL LFADINGKLY HDSQNMLRGE
    1260 1270 1280 1290 1300
    DMSFLEKDAP YMVGDVITSG DITCVVIPSK KAGGTTEMLS RALKKVPVDE
    1310 1320 1330 1340 1350
    YITTYPGQGC AGYTLEEAKT ALKKCKSAFY VLPSEAPNAK EEILGTVSWN
    1360 1370 1380 1390 1400
    LREMLAHAEE TRKLMPICMD VRAIMATIQR KYKGIKIQEG IVDYGVRFFF
    1410 1420 1430 1440 1450
    YTSKEPVASI ITKLNSLNEP LVTMPIGYVT HGFNLEEAAR CMRSLKAPAV
    1460 1470 1480 1490 1500
    VSVSSPDAVT TYNGYLTSSS KTSEEHFVET VSLAGSYRDW SYSGQRTELG
    1510 1520 1530 1540 1550
    VEFLKRGDKI VYHTLESPVE FHLDGEVLSL DKLKSLLSLR EVKTIKVFTT
    1560 1570 1580 1590 1600
    VDNTNLHTQL VDMSMTYGQQ FGPTYLDGAD VTKIKPHVNH EGKTFFVLPS
    1610 1620 1630 1640 1650
    DDTLRSEAFE YYHTLDESFL GRYMSALNHT KKWKFPQVGG LTSIKWADNN
    1660 1670 1680 1690 1700
    CYLSSVLLAL QQLEVKFNAP ALQEAYYRAR AGDAANFCAL ILAYSNKTVG
    1710 1720 1730 1740 1750
    ELGDVRETMT HLLQHANLES AKRVLNVVCK HCGQKTTTLT GVEAVMYMGT
    1760 1770 1780 1790 1800
    LSYDNLKTGV SIPCVCGRDA TQYLVQQESS FVMMSAPPAE YKLQQGTFLC
    1810 1820 1830 1840 1850
    ANEYTGNYQC GHYTHITAKE TLYRIDGAHL TKMSEYKGPV TDVFYKETSY
    1860 1870 1880 1890 1900
    TTTIKPVSYK LDGVTYTEIE PKLDGYYKKD NAYYTEQPID LVPTQPLPNA
    1910 1920 1930 1940 1950
    SFDNFKLTCS NTKFADDLNQ MTGFTKPASR ELSVTFFPDL NGDVVAIDYR
    1960 1970 1980 1990 2000
    HYSASFKKGA KLLHKPIVWH INQATTKTTF KPNTWCLRCL WSTKPVDTSN
    2010 2020 2030 2040 2050
    SFEVLAVEDT QGMDNLACES QQPTSEEVVE NPTIQKEVIE CDVKTTEVVG
    2060 2070 2080 2090 2100
    NVILKPSDEG VKVTQELGHE DLMAAYVENT SITIKKPNEL SLALGLKTIA
    2110 2120 2130 2140 2150
    THGIAAINSV PWSKILAYVK PFLGQAAITT SNCAKRLAQR VFNNYMPYVF
    2160 2170 2180 2190 2200
    TLLFQLCTFT KSTNSRIRAS LPTTIAKNSV KSVAKLCLDA GINYVKSPKF
    2210 2220 2230 2240 2250
    SKLFTIAMWL LLLSICLGSL ICVTAAFGVL LSNFGAPSYC NGVRELYLNS
    2260 2270 2280 2290 2300
    SNVTTMDFCE GSFPCSICLS GLDSLDSYPA LETIQVTISS YKLDLTILGL
    2310 2320 2330 2340 2350
    AAEWVLAYML FTKFFYLLGL SAIMQVFFGY FASHFISNSW LMWFIISIVQ
    2360 2370 2380 2390 2400
    MAPVSAMVRM YIFFASFYYI WKSYVHIMDG CTSSTCMMCY KRNRATRVEC
    2410 2420 2430 2440 2450
    TTIVNGMKRS FYVYANGGRG FCKTHNWNCL NCDTFCTGST FISDEVARDL
    2460 2470 2480 2490 2500
    SLQFKRPINP TDQSSYIVDS VAVKNGALHL YFDKAGQKTY ERHPLSHFVN
    2510 2520 2530 2540 2550
    LDNLRANNTK GSLPINVIVF DGKSKCDESA SKSASVYYSQ LMCQPILLLD
    2560 2570 2580 2590 2600
    QALVSDVGDS TEVSVKMFDA YVDTFSATFS VPMEKLKALV ATAHSELAKG
    2610 2620 2630 2640 2650
    VALDGVLSTF VSAARQGVVD TDVDTKDVIE CLKLSHHSDL EVTGDSCNNF
    2660 2670 2680 2690 2700
    MLTYNKVENM TPRDLGACID CNARHINAQV AKSHNVSLIW NVKDYMSLSE
    2710 2720 2730 2740 2750
    QLRKQIRSAA KKNNIPFRLT CATTRQVVNV ITTKISLKGG KIVSTCFKLM
    2760 2770 2780 2790 2800
    LKATLLCVLA ALVCYIVMPV HTLSIHDGYT NEIIGYKAIQ DGVTRDIIST
    2810 2820 2830 2840 2850
    DDCFANKHAG FDAWFSQRGG SYKNDKSCPV VAAIITREIG FIVPGLPGTV
    2860 2870 2880 2890 2900
    LRAINGDFLH FLPRVFSAVG NICYTPSKLI EYSDFATSAC VLAAECTIFK
    2910 2920 2930 2940 2950
    DAMGKPVPYC YDTNLLEGSI SYSELRPDTR YVLMDGSIIQ FPNTYLEGSV
    2960 2970 2980 2990 3000
    RVVTTFDAEY CRHGTCERSE VGICLSTSGR WVLNNEHYRA LSGVFCGVDA
    3010 3020 3030 3040 3050
    MNLIANIFTP LVQPVGALDV SASVVAGGII AILVTCAAYY FMKFRRVFGE
    3060 3070 3080 3090 3100
    YNHVVAANAL LFLMSFTILC LVPAYSFLPG VYSVFYLYLT FYFTNDVSFL
    3110 3120 3130 3140 3150
    AHLQWFAMFS PIVPFWITAI YVFCISLKHC HWFFNNYLRK RVMFNGVTFS
    3160 3170 3180 3190 3200
    TFEEAALCTF LLNKEMYLKL RSETLLPLTQ YNRYLALYNK YKYFSGALDT
    3210 3220 3230 3240 3250
    TSYREAACCH LAKALNDFSN SGADVLYQPP QTSITSAVLQ SGFRKMAFPS
    3260 3270 3280 3290 3300
    GKVEGCMVQV TCGTTTLNGL WLDDTVYCPR HVICTAEDML NPNYEDLLIR
    3310 3320 3330 3340 3350
    KSNHSFLVQA GNVQLRVIGH SMQNCLLRLK VDTSNPKTPK YKFVRIQPGQ
    3360 3370 3380 3390 3400
    TFSVLACYNG SPSGVYQCAM RPNHTIKGSF LNGSCGSVGF NIDYDCVSFC
    3410 3420 3430 3440 3450
    YMHHMELPTG VHAGTDLEGK FYGPFVDRQT AQAAGTDTTI TLNVLAWLYA
    3460 3470 3480 3490 3500
    AVINGDRWFL NRFTTTLNDF NLVAMKYNYE PLTQDHVDIL GPLSAQTGIA
    3510 3520 3530 3540 3550
    VLDMCAALKE LLQNGMNGRT ILGSTILEDE FTPFDVVRQC SGVTFQGKFK
    3560 3570 3580 3590 3600
    KIVKGTHHWM LLTFLTSLLI LVQSTQWSLF FFVYENAFLP FTLGIMAIAA
    3610 3620 3630 3640 3650
    CAMLLVKHKH AFLCLFLLPS LATVAYFNMV YMPASWVMRI MTWLELADTS
    3660 3670 3680 3690 3700
    LSGYRLKDCV MYASALVLLI LMTARTVYDD AARRVWTLMN VITLVYKVYY
    3710 3720 3730 3740 3750
    GNALDQAISM WALVISVTSN YSGVVTTIMF LARAIVFVCV EYYPLLFITG
    3760 3770 3780 3790 3800
    NTLQCIMLVY CFLGYCCCCY FGLFCLLNRY FRLTLGVYDY LVSTQEFRYM
    3810 3820 3830 3840 3850
    NSQGLLPPKS SIDAFKLNIK LLGIGGKPCI KVATVQSKMS DVKCTSVVLL
    3860 3870 3880 3890 3900
    SVLQQLRVES SSKLWAQCVQ LHNDILLAKD TTEAFEKMVS LLSVLLSMQG
    3910 3920 3930 3940 3950
    AVDINRLCEE MLDNRATLQA IASEFSSLPS YAAYATAQEA YEQAVANGDS
    3960 3970 3980 3990 4000
    EVVLKKLKKS LNVAKSEFDR DAAMQRKLEK MADQAMTQMY KQARSEDKRA
    4010 4020 4030 4040 4050
    KVTSAMQTML FTMLRKLDND ALNNIINNAR DGCVPLNIIP LTTAAKLMVV
    4060 4070 4080 4090 4100
    VPDYGTYKNT CDGNTFTYAS ALWEIQQVVD ADSKIVQLSE INMDNSPNLA
    4110 4120 4130 4140 4150
    WPLIVTALRA NSAVKLQNNE LSPVALRQMS CAAGTTQTAC TDDNALAYYN
    4160 4170 4180 4190 4200
    NSKGGRFVLA LLSDHQDLKW ARFPKSDGTG TIYTELEPPC RFVTDTPKGP
    4210 4220 4230 4240 4250
    KVKYLYFIKG LNNLNRGMVL GSLAATVRLQ AGNATEVPAN STVLSFCAFA
    4260 4270 4280 4290 4300
    VDPAKAYKDY LASGGQPITN CVKMLCTHTG TGQAITVTPE ANMDQESFGG
    4310 4320 4330 4340 4350
    ASCCLYCRCH IDHPNPKGFC DLKGKYVQIP TTCANDPVGF TLRNTVCTVC
    4360 4370 4380 4390 4400
    GMWKGYGCSC DQLREPLMQS ADASTFLNRV CGVSAARLTP CGTGTSTDVV
    4410 4420 4430 4440 4450
    YRAFDIYNEK VAGFAKFLKT NCCRFQEKDE EGNLLDSYFV VKRHTMSNYQ
    4460 4470 4480 4490 4500
    HEETIYNLVK DCPAVAVHDF FKFRVDGDMV PHISRQRLTK YTMADLVYAL
    4510 4520 4530 4540 4550
    RHFDEGNCDT LKEILVTYNC CDDDYFNKKD WYDFVENPDI LRVYANLGER
    4560 4570 4580 4590 4600
    VRQSLLKTVQ FCDAMRDAGI VGVLTLDNQD LNGNWYDFGD FVQVAPGCGV
    4610 4620 4630 4640 4650
    PIVDSYYSLL MPILTLTRAL AAESHMDADL AKPLIKWDLL KYDFTEERLC
    4660 4670 4680 4690 4700
    LFDRYFKYWD QTYHPNCINC LDDRCILHCA NFNVLFSTVF PPTSFGPLVR
    4710 4720 4730 4740 4750
    KIFVDGVPFV VSTGYHFREL GVVHNQDVNL HSSRLSFKEL LVYAADPAMH
    4760 4770 4780 4790 4800
    AASGNLLLDK RTTCFSVAAL TNNVAFQTVK PGNFNKDFYD FAVSKGFFKE
    4810 4820 4830 4840 4850
    GSSVELKHFF FAQDGNAAIS DYDYYRYNLP TMCDIRQLLF VVEVVDKYFD
    4860 4870 4880 4890 4900
    CYDGGCINAN QVIVNNLDKS AGFPFNKWGK ARLYYDSMSY EDQDALFAYT
    4910 4920 4930 4940 4950
    KRNVIPTITQ MNLKYAISAK NRARTVAGVS ICSTMTNRQF HQKLLKSIAA
    4960 4970 4980 4990 5000
    TRGATVVIGT SKFYGGWHNM LKTVYSDVET PHLMGWDYPK CDRAMPNMLR
    5010 5020 5030 5040 5050
    IMASLVLARK HNTCCNLSHR FYRLANECAQ VLSEMVMCGG SLYVKPGGTS
    5060 5070 5080 5090 5100
    SGDATTAYAN SVFNICQAVT ANVNALLSTD GNKIADKYVR NLQHRLYECL
    5110 5120 5130 5140 5150
    YRNRDVDHEF VDEFYAYLRK HFSMMILSDD AVVCYNSNYA AQGLVASIKN
    5160 5170 5180 5190 5200
    FKAVLYYQNN VFMSEAKCWT ETDLTKGPHE FCSQHTMLVK QGDDYVYLPY
    5210 5220 5230 5240 5250
    PDPSRILGAG CFVDDIVKTD GTLMIERFVS LAIDAYPLTK HPNQEYADVF
    5260 5270 5280 5290 5300
    HLYLQYIRKL HDELTGHMLD MYSVMLTNDN TSRYWEPEFY EAMYTPHTVL
    5310 5320 5330 5340 5350
    QAVGACVLCN SQTSLRCGAC IRRPFLCCKC CYDHVISTSH KLVLSVNPYV
    5360 5370 5380 5390 5400
    CNAPGCDVTD VTQLYLGGMS YYCKSHKPPI SFPLCANGQV FGLYKNTCVG
    5410 5420 5430 5440 5450
    SDNVTDFNAI ATCDWTNAGD YILANTCTER LKLFAAETLK ATEETFKLSY
    5460 5470 5480 5490 5500
    GIATVREVLS DRELHLSWEV GKPRPPLNRN YVFTGYRVTK NSKVQIGEYT
    5510 5520 5530 5540 5550
    FEKGDYGDAV VYRGTTTYKL NVGDYFVLTS HTVMPLSAPT LVPQEHYVRI
    5560 5570 5580 5590 5600
    TGLYPTLNIS DEFSSNVANY QKVGMQKYST LQGPPGTGKS HFAIGLALYY
    5610 5620 5630 5640 5650
    PSARIVYTAC SHAAVDALCE KALKYLPIDK CSRIIPARAR VECFDKFKVN
    5660 5670 5680 5690 5700
    STLEQYVFCT VNALPETTAD IVVFDEISMA TNYDLSVVNA RLRAKHYVYI
    5710 5720 5730 5740 5750
    GDPAQLPAPR TLLTKGTLEP EYFNSVCRLM KTIGPDMFLG TCRRCPAEIV
    5760 5770 5780 5790 5800
    DTVSALVYDN KLKAHKDKSA QCFKMFYKGV ITHDVSSAIN RPQIGVVREF
    5810 5820 5830 5840 5850
    LTRNPAWRKA VFISPYNSQN AVASKILGLP TQTVDSSQGS EYDYVIFTQT
    5860 5870 5880 5890 5900
    TETAHSCNVN RFNVAITRAK IGILCIMSDR DLYDKLQFTS LEIPRRNVAT
    5910 5920 5930 5940 5950
    LQAENVTGLF KDCSKIITGL HPTQAPTHLS VDIKFKTEGL CVDIPGIPKD
    5960 5970 5980 5990 6000
    MTYRRLISMM GFKMNYQVNG YPNMFITREE AIRHVRAWIG FDVEGCHATR
    6010 6020 6030 6040 6050
    DAVGTNLPLQ LGFSTGVNLV AVPTGYVDTE NNTEFTRVNA KPPPGDQFKH
    6060 6070 6080 6090 6100
    LIPLMYKGLP WNVVRIKIVQ MLSDTLKGLS DRVVFVLWAH GFELTSMKYF
    6110 6120 6130 6140 6150
    VKIGPERTCC LCDKRATCFS TSSDTYACWN HSVGFDYVYN PFMIDVQQWG
    6160 6170 6180 6190 6200
    FTGNLQSNHD QHCQVHGNAH VASCDAIMTR CLAVHECFVK RVDWSVEYPI
    6210 6220 6230 6240 6250
    IGDELRVNSA CRKVQHMVVK SALLADKFPV LHDIGNPKAI KCVPQAEVEW
    6260 6270 6280 6290 6300
    KFYDAQPCSD KAYKIEELFY SYATHHDKFT DGVCLFWNCN VDRYPANAIV
    6310 6320 6330 6340 6350
    CRFDTRVLSN LNLPGCDGGS LYVNKHAFHT PAFDKSAFTN LKQLPFFYYS
    6360 6370 6380 6390 6400
    DSPCESHGKQ VVSDIDYVPL KSATCITRCN LGGAVCRHHA NEYRQYLDAY
    6410 6420 6430 6440 6450
    NMMISAGFSL WIYKQFDTYN LWNTFTRLQS LENVAYNVVN KGHFDGHAGE
    6460 6470 6480 6490 6500
    APVSIINNAV YTKVDGIDVE IFENKTTLPV NVAFELWAKR NIKPVPEIKI
    6510 6520 6530 6540 6550
    LNNLGVDIAA NTVIWDYKRE APAHVSTIGV CTMTDIAKKP TESACSSLTV
    6560 6570 6580 6590 6600
    LFDGRVEGQV DLFRNARNGV LITEGSVKGL TPSKGPAQAS VNGVTLIGES
    6610 6620 6630 6640 6650
    VKTQFNYFKK VDGIIQQLPE TYFTQSRDLE DFKPRSQMET DFLELAMDEF
    6660 6670 6680 6690 6700
    IQRYKLEGYA FEHIVYGDFS HGQLGGLHLM IGLAKRSQDS PLKLEDFIPM
    6710 6720 6730 6740 6750
    DSTVKNYFIT DAQTGSSKCV CSVIDLLLDD FVEIIKSQDL SVISKVVKVT
    6760 6770 6780 6790 6800
    IDYAEISFML WCKDGHVETF YPKLQASQAW QPGVAMPNLY KMQRMLLEKC
    6810 6820 6830 6840 6850
    DLQNYGENAV IPKGIMMNVA KYTQLCQYLN TLTLAVPYNM RVIHFGAGSD
    6860 6870 6880 6890 6900
    KGVAPGTAVL RQWLPTGTLL VDSDLNDFVS DADSTLIGDC ATVHTANKWD
    6910 6920 6930 6940 6950
    LIISDMYDPR TKHVTKENDS KEGFFTYLCG FIKQKLALGG SIAVKITEHS
    6960 6970 6980 6990 7000
    WNADLYKLMG HFSWWTAFVT NVNASSSEAF LIGANYLGKP KEQIDGYTMH
    7010 7020 7030 7040 7050
    ANYIFWRNTN PIQLSSYSLF DMSKFPLKLR GTAVMSLKEN QINDMIYSLL
    7060 7070
    EKGRLIIREN NRVVVSSDIL VNN

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:7,073
    Mass (Da):790,248
    Last modified:June 10, 2008 - v1
    Checksum:iE6504CAFDC36BC09
    GO
    Isoform Replicase polyprotein 1a (identifier: P0C6U8-1) [UniParc]FASTAAdd to basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform can be found in the external entry P0C6U8.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by conventional translation.

    Length:4,382
    Mass (Da):486,373
    GO

    Sequence cautioni

    The sequence AAP13440.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence AAP41036.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence AAP82975.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence AAP97881.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence AAQ01596.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence AAQ01608.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti82 – 821G → C in strain: Isolate GD01.
    Natural varianti130 – 1301G → R in strain: Isolate GD01.
    Natural varianti138 – 1381I → T in strain: Isolate SZ16.
    Natural varianti181 – 1811A → V in strain: Isolate Shanghai LY.
    Natural varianti225 – 2251K → Q in strain: Isolate GD01.
    Natural varianti249 – 2491Y → C in strain: Isolate Shanghai LY.
    Natural varianti306 – 3061V → F in strain: Isolate BJ04.
    Natural varianti549 – 5491A → S in strain: Isolate SZ3.
    Natural varianti765 – 7651A → T in strain: Isolate FRA and Isolate Frankfurt-1.
    Natural varianti852 – 8521K → R in strain: Isolate SZ16.
    Natural varianti1004 – 10041N → H in strain: Isolate BJ03.
    Natural varianti1021 – 10211V → A in strain: Isolate SZ3 and Isolate SZ16.
    Natural varianti1023 – 10231I → T in strain: Isolate Shanghai QXC1.
    Natural varianti1121 – 11211I → T in strain: Isolate GD01, Isolate SZ3 and Isolate SZ16.
    Natural varianti1136 – 11361P → L in strain: Isolate SZ3 and Isolate SZ16.
    Natural varianti1257 – 12571K → E in strain: Isolate Shanghai QXC1.
    Natural varianti1319 – 13191K → R in strain: Isolate GD01.
    Natural varianti1329 – 13291F → S in strain: Isolate GD01.
    Natural varianti1361 – 13611T → A in strain: Isolate Shanghai QXC1.
    Natural varianti1385 – 13851I → V in strain: Isolate Shanghai QXC1.
    Natural varianti1538 – 15381S → T in strain: Isolate GD01.
    Natural varianti1563 – 15631M → K in strain: Isolate BJ02.
    Natural varianti1663 – 16631L → I in strain: Isolate SZ3 and Isolate SZ16.
    Natural varianti1762 – 17621I → L in strain: Isolate BJ03.
    Natural varianti1776 – 17772QQ → PP in strain: Isolate BJ03.
    Natural varianti1790 – 17901E → G in strain: Isolate Shanghai QXC1.
    Natural varianti1806 – 18061G → V in strain: Isolate BJ02.
    Natural varianti1962 – 19621L → I in strain: Isolate BJ04.
    Natural varianti2116 – 21161L → F in strain: Isolate GD01, Isolate SZ3 and Isolate SZ16.
    Natural varianti2222 – 22221C → Y in strain: Isolate GD01, Isolate SZ3 and Isolate SZ16.
    Natural varianti2269 – 22691L → S in strain: Isolate SZ3 and Isolate SZ16.
    Natural varianti2326 – 23261V → A in strain: Isolate Shanghai QXC1.
    Natural varianti2392 – 23943RNR → CNH in strain: Isolate Shanghai QXC1.
    Natural varianti2480 – 24801L → P in strain: Isolate Shanghai QXC1.
    Natural varianti2552 – 25521A → V in strain: Isolate Urbani and Isolate Taiwan TC2.
    Natural varianti2556 – 25561D → N in strain: Isolate HKU-39849.
    Natural varianti2564 – 25641S → P in strain: Isolate GD01.
    Natural varianti2648 – 26481N → Y in strain: Isolate Shanghai QXC1.
    Natural varianti2708 – 27081S → T in strain: Isolate HKU-39849.
    Natural varianti2718 – 27181R → T in strain: Isolate HKU-39849.
    Natural varianti2746 – 27461C → W in strain: Isolate SZ3 and Isolate SZ16.
    Natural varianti2770 – 27701V → L in strain: Isolate BJ01 and Isolate BJ02.
    Natural varianti2944 – 29441T → I in strain: Isolate SIN2500, Isolate GD01 and Isolate GZ50.
    Natural varianti2971 – 29711V → A in strain: Isolate GD01 and Isolate SZ16.
    Natural varianti3020 – 30201V → A in strain: Isolate Shanghai QXC1.
    Natural varianti3047 – 30471V → A in strain: Isolate CUHK-W1, Isolate GD01, Isolate SZ3, Isolate SZ16, Isolate BJ01, Isolate BJ02, Isolate BJ03 and Isolate Shanghai QXC1.
    Natural varianti3072 – 30721V → A in strain: Isolate CUHK-W1, Isolate SZ3, Isolate SZ16 and Isolate GD01.
    Natural varianti3197 – 31971A → V in strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate Shanghai QXC1.
    Natural varianti3429 – 34291Q → P in strain: Isolate BJ02.
    Natural varianti3488 – 34881D → E in strain: Isolate BJ04.
    Natural varianti3717 – 37171V → A in strain: Isolate Shanghai QXC1.
    Natural varianti3818 – 38181N → T in strain: Isolate BJ04.
    Natural varianti3903 – 39031D → N in strain: Isolate BJ03.
    Natural varianti3904 – 39041I → F in strain: Isolate BJ02.
    Natural varianti3911 – 39111M → V in strain: Isolate Shanghai QXC1.
    Natural varianti4001 – 40011K → Q in strain: Isolate Shanghai LY.
    Natural varianti4003 – 40031T → A in strain: Isolate Shanghai LY.
    Natural varianti4085 – 40851I → H in strain: Isolate ZJ01.
    Natural varianti4114 – 41141V → A in strain: Isolate Shanghai QXC1.
    Natural varianti4202 – 42021V → M in strain: Isolate Shanghai QXC1.
    Natural varianti4240 – 42401N → H in strain: Isolate ZJ01.
    Natural varianti4296 – 42961E → G in strain: Isolate Shanghai QXC1.
    Natural varianti4377 – 43782LN → FK in strain: Isolate Shanghai QXC1.
    Natural varianti4411 – 44111V → S in strain: Isolate HKU-39849.
    Natural varianti4459 – 44591V → I in strain: Isolate Shanghai QXC1.
    Natural varianti4592 – 45921V → E in strain: Isolate ZJ01.
    Natural varianti4910 – 49101Q → L in strain: Isolate ZJ01.
    Natural varianti5112 – 51121D → G in strain: Isolate SZ3.
    Natural varianti5131 – 51311A → G in strain: Isolate Taiwan.
    Natural varianti5134 – 51352CY → VL in strain: Isolate Taiwan.
    Natural varianti5623 – 56231L → S in strain: Isolate GD01.
    Natural varianti5720 – 57201P → S in strain: Isolate GZ50 and Isolate SIN2500.
    Natural varianti5744 – 57441R → C in strain: Isolate ZJ01.
    Natural varianti5767 – 57671D → E in strain: Isolate CUHK-W1, Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04, Isolate SIN2500, Isolate GD01, Isolate GZ50, Isolate SZ3, Isolate SZ16 and Isolate Shanghai QXC1.
    Natural varianti6274 – 62741T → I in strain: Isolate FRA, Isolate Frankfurt-1 Isolate SIN2677, Isolate SIN2679 and Isolate SIN2748.
    Natural varianti6474 – 64741N → S in strain: Isolate Shanghai QXC1.
    Natural varianti6700 – 67001M → I in strain: Isolate BJ03.
    Natural varianti6721 – 67211C → R in strain: Isolate Shanghai QXC1.
    Natural varianti6729 – 67291D → N in strain: Isolate GD01.
    Natural varianti6840 – 68401M → L in strain: Isolate BJ02.
    Natural varianti6862 – 68621Q → P in strain: Isolate BJ04.
    Natural varianti6877 – 68771D → E in strain: Isolate GD01.
    Natural varianti6910 – 69101R → K in strain: Isolate SZ3 and Isolate SZ16.
    Natural varianti6937 – 69371A → P in strain: Isolate BJ03.
    Natural varianti6992 – 69921E → D in strain: Isolate BJ04.
    Natural varianti7008 – 70081N → K in strain: Isolate GD01.
    Natural varianti7024 – 70241K → Q in strain: Isolate BJ04.

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY278741 Genomic RNA. Translation: AAP13442.1.
    AY278741 Genomic RNA. Translation: AAP13440.1. Sequence problems.
    AY274119 Genomic RNA. Translation: AAP41036.1. Sequence problems.
    AY278554 Genomic RNA. Translation: AAP13566.1.
    AY282752 Genomic RNA. Translation: AAP30711.1.
    AY304495 Genomic RNA. No translation available.
    AY304486 Genomic RNA. No translation available.
    AY304488 Genomic RNA. No translation available.
    AY278491 Genomic RNA. No translation available.
    AY283794 Genomic RNA. No translation available.
    AY283795 Genomic RNA. No translation available.
    AY283796 Genomic RNA. No translation available.
    AY283797 Genomic RNA. No translation available.
    AY283798 Genomic RNA. No translation available.
    AY286320 Genomic RNA. Translation: AAP49011.4.
    AY278488 Genomic RNA. Translation: AAP30028.1.
    AY278489 Genomic RNA. Translation: AAP51225.1.
    AY278490 Genomic RNA. No translation available.
    AY279354 Genomic RNA. No translation available.
    AY291451 Genomic RNA. Translation: AAP37015.1.
    AY310120 Genomic RNA. Translation: AAP50483.1.
    AY291315 Genomic RNA. Translation: AAP33696.1.
    AY323977 Genomic RNA. Translation: AAP72973.2.
    AY321118 Genomic RNA. No translation available.
    AY338174 Genomic RNA. Translation: AAQ01594.1.
    AY338174 Genomic RNA. Translation: AAQ01596.1. Sequence problems.
    AY338175 Genomic RNA. Translation: AAQ01606.1.
    AY338175 Genomic RNA. Translation: AAQ01608.1. Sequence problems.
    AY348314 Genomic RNA. Translation: AAP97879.1.
    AY348314 Genomic RNA. Translation: AAP97881.1. Sequence problems.
    AP006557 Genomic RNA. Translation: BAC81346.1.
    AP006558 Genomic RNA. Translation: BAC81360.1.
    AP006559 Genomic RNA. Translation: BAC81374.1.
    AP006560 Genomic RNA. Translation: BAC81388.1.
    AP006561 Genomic RNA. Translation: BAC81402.1.
    AY427439 Genomic RNA. Translation: AAQ94058.1.
    AY322205 Genomic RNA. Translation: AAP82966.1.
    AY322206 Genomic RNA. Translation: AAP82975.1. Sequence problems.
    AY322207 Genomic RNA. Translation: AAP82967.1.
    AY463059 Genomic RNA. Translation: AAP82978.2.
    AY269391 Genomic RNA. Translation: AAP04003.1.
    AY268049 Genomic RNA. Translation: AAP04587.1.
    RefSeqiNP_828849.2. NC_004718.3. [P0C6X7-1]

    Genome annotation databases

    GeneIDi1489680.
    KEGGivg:1489680.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY278741 Genomic RNA. Translation: AAP13442.1.
    AY278741 Genomic RNA. Translation: AAP13440.1. Sequence problems.
    AY274119 Genomic RNA. Translation: AAP41036.1. Sequence problems.
    AY278554 Genomic RNA. Translation: AAP13566.1.
    AY282752 Genomic RNA. Translation: AAP30711.1.
    AY304495 Genomic RNA. No translation available.
    AY304486 Genomic RNA. No translation available.
    AY304488 Genomic RNA. No translation available.
    AY278491 Genomic RNA. No translation available.
    AY283794 Genomic RNA. No translation available.
    AY283795 Genomic RNA. No translation available.
    AY283796 Genomic RNA. No translation available.
    AY283797 Genomic RNA. No translation available.
    AY283798 Genomic RNA. No translation available.
    AY286320 Genomic RNA. Translation: AAP49011.4.
    AY278488 Genomic RNA. Translation: AAP30028.1.
    AY278489 Genomic RNA. Translation: AAP51225.1.
    AY278490 Genomic RNA. No translation available.
    AY279354 Genomic RNA. No translation available.
    AY291451 Genomic RNA. Translation: AAP37015.1.
    AY310120 Genomic RNA. Translation: AAP50483.1.
    AY291315 Genomic RNA. Translation: AAP33696.1.
    AY323977 Genomic RNA. Translation: AAP72973.2.
    AY321118 Genomic RNA. No translation available.
    AY338174 Genomic RNA. Translation: AAQ01594.1.
    AY338174 Genomic RNA. Translation: AAQ01596.1. Sequence problems.
    AY338175 Genomic RNA. Translation: AAQ01606.1.
    AY338175 Genomic RNA. Translation: AAQ01608.1. Sequence problems.
    AY348314 Genomic RNA. Translation: AAP97879.1.
    AY348314 Genomic RNA. Translation: AAP97881.1. Sequence problems.
    AP006557 Genomic RNA. Translation: BAC81346.1.
    AP006558 Genomic RNA. Translation: BAC81360.1.
    AP006559 Genomic RNA. Translation: BAC81374.1.
    AP006560 Genomic RNA. Translation: BAC81388.1.
    AP006561 Genomic RNA. Translation: BAC81402.1.
    AY427439 Genomic RNA. Translation: AAQ94058.1.
    AY322205 Genomic RNA. Translation: AAP82966.1.
    AY322206 Genomic RNA. Translation: AAP82975.1. Sequence problems.
    AY322207 Genomic RNA. Translation: AAP82967.1.
    AY463059 Genomic RNA. Translation: AAP82978.2.
    AY269391 Genomic RNA. Translation: AAP04003.1.
    AY268049 Genomic RNA. Translation: AAP04587.1.
    RefSeqiNP_828849.2. NC_004718.3. [P0C6X7-1]

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1O5Smodel-A4745-5301[»]
    1P76model-A3241-3541[»]
    B4225-4231[»]
    1P9Tmodel-A3241-3544[»]
    1PA5model-A3241-3546[»]
    1PUKmodel-A3241-3550[»]
    1Q1Xmodel-A3241-3542[»]
    1Q2WX-ray1.86A/B3241-3544[»]
    1QZ8X-ray2.70A/B4118-4230[»]
    1SXFmodel-A4765-5244[»]
    1UJ1X-ray1.90A/B3241-3546[»]
    1UK2X-ray2.20A/B3241-3546[»]
    1UK3X-ray2.40A/B3241-3546[»]
    1UK4X-ray2.50A/B3241-3546[»]
    1UW7X-ray2.80A4118-4230[»]
    1WOFX-ray2.00A/B3241-3546[»]
    1YSYNMR-A3837-3919[»]
    1Z1IX-ray2.80A3241-3546[»]
    1Z1JX-ray2.80A/B3241-3546[»]
    2A5AX-ray2.08A3241-3546[»]
    2A5IX-ray1.88A3241-3546[»]
    2A5KX-ray2.30A/B3241-3546[»]
    2ACFX-ray1.40A/B/C/D1002-1176[»]
    2AHMX-ray2.40A/B/C/D3837-3919[»]
    E/F/G/H3920-4117[»]
    2AJ5model-A3241-3546[»]
    2ALVX-ray1.90A3241-3543[»]
    2AMDX-ray1.85A/B3241-3546[»]
    2AMQX-ray2.30A/B3241-3546[»]
    2BX3X-ray2.00A3241-3546[»]
    2BX4X-ray2.79A3241-3546[»]
    2C3SX-ray1.90A3241-3546[»]
    2D2DX-ray2.70A/B3241-3546[»]
    2DUCX-ray1.70A/B3241-3546[»]
    2FAVX-ray1.80A/B/C1000-1173[»]
    2FE8X-ray1.85A/B/C1541-1854[»]
    2FYGX-ray1.80A4240-4362[»]
    2G1Fmodel-A/B5302-5877[»]
    2G9TX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X4231-4378[»]
    2GA6X-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X4231-4378[»]
    2GDTNMR-A13-127[»]
    2GRINMR-A819-930[»]
    2GT7X-ray1.82A/B3241-3546[»]
    2GT8X-ray2.00A3241-3546[»]
    2GTBX-ray2.00A3241-3546[»]
    2GX4X-ray1.93A3241-3546[»]
    2GZ7X-ray1.86A3241-3546[»]
    2GZ8X-ray1.97A3241-3546[»]
    2GZ9X-ray2.17A3241-3546[»]
    2H2ZX-ray1.60A3241-3546[»]
    2H85X-ray2.60A6429-6774[»]
    2HOBX-ray1.95A3241-3546[»]
    2HSXNMR-A13-127[»]
    2IDYNMR-A819-930[»]
    2JZDNMR-A1345-1469[»]
    2JZENMR-A1345-1469[»]
    2JZFNMR-A1331-1469[»]
    2K87NMR-A1884-1998[»]
    2OP9X-ray1.80A/B3241-3541[»]
    2OZKX-ray2.90A/B/C/D6430-6775[»]
    2PWXX-ray2.50A3241-3546[»]
    2Q6GX-ray2.50A/B3241-3546[»]
    2QC2X-ray2.70A/B3241-3546[»]
    2QCYX-ray1.75A3241-3546[»]
    2QIQX-ray1.90A3242-3541[»]
    2RHBX-ray2.80A/B/C/D/E/F6430-6775[»]
    2RNKNMR-A1331-1469[»]
    2V6NX-ray1.98A3241-3546[»]
    2VJ1X-ray2.25A/B3242-3544[»]
    2XYQX-ray2.00A6776-7065[»]
    B4240-4361[»]
    2XYRX-ray2.50A6776-7067[»]
    B4240-4361[»]
    2XYVX-ray2.06A6776-7067[»]
    B4240-4361[»]
    2YY4X-ray2.20A/B3241-3556[»]
    2Z3CX-ray1.79A3241-3546[»]
    2Z3DX-ray2.10A3241-3546[»]
    2Z3EX-ray2.32A3241-3546[»]
    2Z94X-ray1.78A3241-3546[»]
    2Z9GX-ray1.86A3241-3546[»]
    2Z9JX-ray1.95A/B3241-3546[»]
    2Z9KX-ray1.85A/B3241-3546[»]
    2Z9LX-ray2.10A/B3241-3546[»]
    3D62X-ray2.70A3243-3541[»]
    3E9SX-ray2.50A1541-1855[»]
    3EBNX-ray2.40A/B/C/D3429-3546[»]
    3R24X-ray2.00A6776-7073[»]
    B4240-4382[»]
    4TWWX-ray2.42A/B3241-3546[»]
    4TWYX-ray1.60A3241-3546[»]
    4WY3X-ray1.89A3241-3546[»]
    ProteinModelPortaliP0C6X7.
    SMRiP0C6X7. Positions 13-127, 819-930, 1002-1176, 1331-1469, 1541-1854, 3241-3546, 3837-3910, 3921-4111, 4118-4230, 4240-4362, 6430-6773.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    MINTiMINT-1487760.

    Chemistry

    BindingDBiP0C6X7.
    ChEMBLiCHEMBL5118.

    Proteomic databases

    PRIDEiP0C6X7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi1489680.
    KEGGivg:1489680.

    Enzyme and pathway databases

    BRENDAi2.7.7.48. 7599.
    3.4.22.B14. 7599.
    3.6.4.12. 7599.

    Miscellaneous databases

    EvolutionaryTraceiP0C6X7.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Urbani.
    2. "The genome sequence of the SARS-associated coronavirus."
      Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H.
      , Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L.
      Science 300:1399-1404(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Tor2.
    3. "Coronavirus genomic-sequence variations and the epidemiology of the severe acute respiratory syndrome."
      Tsui S.K.W., Chim S.S.C., Lo Y.M.D.
      N. Engl. J. Med. 349:187-188(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate CUHK-Su10 and Isolate CUHK-W1.
    4. "Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China."
      Guan Y., Zheng B.J., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S.W., Li P.H., Zhang L.J., Guan Y.J., Butt K.M., Wong K.L., Chan K.W., Lim W., Shortridge K.F., Yuen K.Y., Peiris J.S.M., Poon L.L.M.
      Science 302:276-278(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate GZ50, Isolate SZ16 and Isolate SZ3.
    5. "The complete genome sequence of severe acute respiratory syndrome coronavirus strain HKU-39849 (HK-39)."
      Zeng F.Y., Chan C.W., Chan M.N., Chen J.D., Chow K.Y.C., Hon C.C.C., Hui R.K.H., Li J., Li V.Y.Y., Wang C.Y., Wang P.Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M., Leung F.C.
      Exp. Biol. Med. 228:866-873(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate HKU-39849.
    6. "Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection."
      Ruan Y., Wei C.L., Ling A.E., Vega V.B., Thoreau H., Se Thoe S.Y., Chia J.-M., Ng P., Chiu K.P., Lim L., Zhang T., Chan K.P., Oon L.E.L., Ng M.L., Leo S.Y., Ng L.F.P., Ren E.C., Stanton L.W., Long P.M., Liu E.T.
      Lancet 361:1779-1785(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Sin2500, Isolate Sin2677, Isolate Sin2679, Isolate Sin2748 and Isolate sin2774.
    7. "Severe acute respiratory syndrome-associated coronavirus genotype and its characterization."
      Li L., Wang Z., Lu Y., Bao Q., Chen S., Wu N., Cheng S., Weng J., Zhang Y., Yan J., Mei L., Wang X., Zhu H., Yu Y., Zhang M., Li M., Yao J., Lu Q.
      , Yao P., Bo X., Wo J., Wang S., Hu S.
      Chin. Med. J. 116:1288-1292(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate ZJ01.
    8. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate BJ01, Isolate BJ02, Isolate BJ03, Isolate BJ04 and Isolate GD01.
    9. "The complete genome of SARS coronavirus clone TW1."
      Yeh S.-H., Kao C.-L., Tsai C.-Y., Liu C.-J., Chen D.-S., Chen P.-J.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TW1.
    10. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate FRA.
    11. Thiel V., Hertzig T., Putics A., Ivanov K.A., Schelle B., Bayer S., Scheiner B., Weinand H., Weissbrich B., Ziebuhr J.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Frankfurt-1.
    12. "Genomic sequence of SARS isolate from the first fatal case in Taiwan."
      Yang J.-Y., Lin J.-H., Chiu S.-C., Wang S.-F., Lee S.C., Lin Y.-C., Hsu C.-K., Chen H.-Y., Chang J.G., Chen P.-J., Su I.-J.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TWC.
    13. "Analysis of SARS coronavirus genome in Shanghai isolates."
      Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Shanghai QXC1.
    14. Canducci F., Clementi M., Poli G., Vicenzi E.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate HSR 1.
    15. Chang J.-G.C., Lin T.-H., Chen C.-M., Lin C.-S., Chan W.-L., Shih M.-C.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Taiwan TC1, Isolate Taiwan TC2 and Isolate Taiwan TC3.
    16. Shu H.Y., Wu K.M., Tsai S.F.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate TWH, Isolate TWJ, Isolate TWK, Isolate TWS and Isolate TWY.
    17. Balotta C., Corvasce S., Violin M., Galli M., Moroni M., Vigevani G.M., Ruan Y.J., Salemi M.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate AS.
    18. Wang Z., Cheng S., Zhang Y.
      Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate ZJ01.
    19. Yuan Z., Zhang X., Hu Y., Lan S., Wang H., Zhou Z., Wen Y.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-507; 1655-5170 AND 6903-7073.
      Strain: Isolate Shanghai LY.
    20. Emery S., Erdman D.D., Peret T.C.T., Ksiazek T.G.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 4993-5127.
      Strain: Isolate Vietnam.
    21. "Detection of a novel human coronavirus in a severe acute respiratory syndrome patient in Taiwan."
      Lin J.-H., Chiu S.-C., Yang J.-Y., Wang S.-F., Chen H.-Y.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 4993-5136.
      Strain: Isolate Taiwan.
    22. "The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases."
      Tanner J.A., Watt R.M., Chai Y.-B., Lu L.-Y., Lin M.C., Peiris J.S., Poon L.L.M., Kung H.-F., Huang J.-D.
      J. Biol. Chem. 278:39578-39582(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION (HELICASE).
    23. Cited for: PROTEOLYTIC PROCESSING (REPLICASE POLYPROTEIN 1AB).
    24. "Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase."
      Fan K., Wei P., Feng Q., Chen S., Huang C., Ma L., Lai B., Pei J., Liu Y., Chen J., Lai L.
      J. Biol. Chem. 279:1637-1642(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY (3C-LIKE PROTEINASE), SUBUNIT (3C-LIKE PROTEINASE), BIOPHYSICOCHEMICAL PROPERTIES (3C-LIKE PROTEINASE).
    25. "Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins."
      Prentice E., McAuliffe J., Lu X., Subbarao K., Denison M.R.
      J. Virol. 78:9977-9986(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING (REPLICASE POLYPROTEIN 1AB).
    26. "Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity."
      Harcourt B.H., Jukneliene D., Kanjanahaluethai A., Bechill J., Severson K.M., Smith C.M., Rota P.A., Baker S.C.
      J. Virol. 78:13600-13612(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING (REPLICASE POLYPROTEIN 1AB).
      Strain: Isolate Urbani.
    27. "Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis."
      Minskaia E., Hertzig T., Gorbalenya A.E., Campanacci V., Cambillau C., Canard B., Ziebuhr J.
      Proc. Natl. Acad. Sci. U.S.A. 103:5108-5113(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION (GUANINE-N7 METHYLTRANSFERASE).
      Strain: Isolate Frankfurt-1.