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P0C6X5

- R1AB_CVHNL

UniProt

P0C6X5 - R1AB_CVHNL

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Protein
Replicase polyprotein 1ab
Gene
rep, 1a-1b
Organism
Human coronavirus NL63 (HCoV-NL63)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.1 Publication
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.1 Publication
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.1 Publication
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G).1 Publication
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.1 Publication
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.1 Publication
Nsp9 is a ssRNA-binding protein By similarity.1 Publication
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei110 – 1112Cleavage; by PL1-PRO By similarity
Sitei898 – 8992Cleavage; by PL1-PRO By similarity
Active sitei1062 – 10621For PL1-PRO activity By similarity
Active sitei1212 – 12121For PL1-PRO activity By similarity
Active sitei1678 – 16781For PL2-PRO activity By similarity
Active sitei1836 – 18361For PL2-PRO activity By similarity
Sitei2462 – 24632Cleavage; by PL2-PRO By similarity
Sitei2939 – 29402Cleavage; by 3CL-PRO By similarity
Active sitei2980 – 29801For 3CL-PRO activity By similarity
Active sitei3083 – 30831For 3CL-PRO activity By similarity
Sitei3242 – 32432Cleavage; by 3CL-PRO By similarity
Sitei3521 – 35222Cleavage; by 3CL-PRO By similarity
Sitei3604 – 36052Cleavage; by 3CL-PRO By similarity
Sitei3799 – 38002Cleavage; by 3CL-PRO By similarity
Sitei3908 – 39092Cleavage; by 3CL-PRO By similarity
Sitei4043 – 40442Cleavage; by 3CL-PRO By similarity
Sitei4970 – 49712Cleavage; by 3CL-PRO By similarity
Sitei6085 – 60862Cleavage; by 3CL-PRO By similarity
Sitei6429 – 64302Cleavage; by 3CL-PRO By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1134 – 116532C4-type 1 By similarity
Add
BLAST
Zinc fingeri1757 – 178832C4-type 2; atypical By similarity
Add
BLAST
Zinc fingeri3982 – 399817 By similarity
Add
BLAST
Zinc fingeri4024 – 403714 By similarity
Add
BLAST
Nucleotide bindingi5253 – 52608ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. endonuclease activity Source: UniProtKB-KW
  6. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  7. helicase activity Source: UniProtKB-KW
  8. methyltransferase activity Source: InterPro
  9. omega peptidase activity Source: InterPro
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of host autophagy Source: UniProtKB-KW
  2. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  3. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral RNA genome replication Source: InterPro
  6. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p9
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p87
Alternative name(s):
PL1-PRO/PL2-PRO
PLP1/PLP2
Papain-like proteinases 1/2
p195
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p34
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p5
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p23
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p14
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
p100
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
p66
p66-HEL
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
p41
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiHuman coronavirus NL63 (HCoV-NL63)
Taxonomic identifieri277944 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008573: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1903 – 192321Helical; Reviewed prediction
Add
BLAST
Transmembranei1968 – 198821Helical; Reviewed prediction
Add
BLAST
Transmembranei2050 – 207021Helical; Reviewed prediction
Add
BLAST
Transmembranei2073 – 209321Helical; Reviewed prediction
Add
BLAST
Transmembranei2111 – 213121Helical; Reviewed prediction
Add
BLAST
Transmembranei2468 – 248821Helical; Reviewed prediction
Add
BLAST
Transmembranei2727 – 274721Helical; Reviewed prediction
Add
BLAST
Transmembranei2752 – 276918Helical; Reviewed prediction
Add
BLAST
Transmembranei2772 – 279221Helical; Reviewed prediction
Add
BLAST
Transmembranei2800 – 282021Helical; Reviewed prediction
Add
BLAST
Transmembranei3254 – 327421Helical; Reviewed prediction
Add
BLAST
Transmembranei3279 – 329921Helical; Reviewed prediction
Add
BLAST
Transmembranei3303 – 332321Helical; Reviewed prediction
Add
BLAST
Transmembranei3342 – 336221Helical; Reviewed prediction
Add
BLAST
Transmembranei3376 – 339621Helical; Reviewed prediction
Add
BLAST
Transmembranei3397 – 341721Helical; Reviewed prediction
Add
BLAST
Transmembranei3442 – 346221Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 110110Non-structural protein 1 By similarity
PRO_0000283874Add
BLAST
Chaini111 – 898788Non-structural protein 2 By similarity
PRO_0000283875Add
BLAST
Chaini899 – 24621564Non-structural protein 3 By similarity
PRO_0000283876Add
BLAST
Chaini2463 – 2939477Non-structural protein 4 By similarity
PRO_0000283877Add
BLAST
Chaini2940 – 32423033C-like proteinase By similarity
PRO_0000283878Add
BLAST
Chaini3243 – 3521279Non-structural protein 6 By similarity
PRO_0000283879Add
BLAST
Chaini3522 – 360483Non-structural protein 7 By similarity
PRO_0000283880Add
BLAST
Chaini3605 – 3799195Non-structural protein 8 By similarity
PRO_0000283881Add
BLAST
Chaini3800 – 3908109Non-structural protein 9 By similarity
PRO_0000283882Add
BLAST
Chaini3909 – 4043135Non-structural protein 10 By similarity
PRO_0000283883Add
BLAST
Chaini4044 – 4970927RNA-directed RNA polymerase By similarity
PRO_0000283884Add
BLAST
Chaini4971 – 5567597Helicase By similarity
PRO_0000283885Add
BLAST
Chaini5568 – 6085518Exoribonuclease By similarity
PRO_0000283886Add
BLAST
Chaini6086 – 6429344Uridylate-specific endoribonuclease By similarity
PRO_0000283887Add
BLAST
Chaini6430 – 6729300Putative 2'-O-methyl transferase By similarity
PRO_0000283888Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6X5.
SMRiP0C6X5. Positions 2940-3240, 3802-3907, 3915-4039.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1021 – 1262242Peptidase C16 1
Add
BLAST
Domaini1263 – 1421159Macro
Add
BLAST
Domaini1640 – 1886247Peptidase C16 2
Add
BLAST
Domaini2940 – 3242303Peptidase C30
Add
BLAST
Domaini4650 – 4812163RdRp catalytic
Add
BLAST
Domaini4971 – 505484CV MBD
Add
BLAST
Domaini5228 – 5409182(+)RNA virus helicase ATP-binding
Add
BLAST
Domaini5410 – 5579170(+)RNA virus helicase C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1903 – 2131229HD1 By similarity
Add
BLAST
Regioni2468 – 2820353HD2 By similarity
Add
BLAST
Regioni3254 – 3462209HD3 By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi625 – 6284Poly-Leu
Compositional biasi1975 – 19784Poly-Leu

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Sequence similaritiesi

Contains 1 Macro domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR011050. Pectin_lyase_fold/virulence.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF51126. SSF51126. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6X5-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MFYNQVTLAV ASDSEISGFG FAIPSVAVRT YSEAAAQGFQ ACRFVAFGLQ     50
DCVTGINDDD YVIALTGTNQ LCAKILPFSD RPLNLRGWLI FSNSNYVLQD 100
FDVVFGHGAG SVVFVDKYMC GFDGKPVLPK NMWEFRDYFN NNTDSIVIGG 150
VTYQLAWDVI RKDLSYEQQN VLAIESIHYL GTTGHTLKSG CKLTNAKPPK 200
YSSKVVLSGE WNAVYRAFGS PFITNGMSLL DIIVKPVFFN AFVKCNCGSE 250
SWSVGAWDGY LSSCCGTPAK KLCVVPGNVV PGDVIITSTS AGCGVKYYAG 300
LVVKHITNIT GVSLWRVTAV HSDGMFVASS SYDALLHRNS LDPFCFDVNT 350
LLSNQLRLAF LGASVTEDVK FAASTGVIDI SAGMFGLYDD ILTNNKPWFV 400
RKASGLFDAI WDAFVAAIKL VPTTTGVLVR FVKSIASTVL TVSNGVIIMC 450
ADVPDAFQSV YRTFTQAICA AFDFSLDVFK IGDVKFKRLG DYVLTENALV 500
RLTTEVVRGV RDARIKKAMF TKVVVGPTTE VKFSVIELAT VNLRLVDCAP 550
VVCPKGKIVV IAGQAFFYSG GFYRFMVDPT TVLNDPVFTG DLFYTIKFSG 600
FKLDGFNHQF VTASSATDAI IAVELLLLDF KTAVFVYTCV VDGCSVIVRR 650
DATFATHVCF KDCYNVWEQF CIDNCGEPWF LTDYNAILQS NNPQCAIVQA 700
SESKVLLERF LPKCPEILLS IDDGHLWNLF VEKFNFVTDW LKTLKLTLTS 750
NGLLGNCAKR FRRVLVKLLD VYNGFLETVC SVAYTAGVCI KYYAVNVPYV 800
VISGFVSRVI RRERCDMTFP CVSCVTFFYE FLDTCFGVSK PNAIDVEHLE 850
LKETVFVEPK DGGQFFVSGD YLWYVVDDIY YPASCNGVLP VAFTKLAGGK 900
ISFSDDVIVH DVEPTHKVKL IFEFEDDVVT SLCKKSFGKS IIYTGDWEGL 950
HEVLTSAMNV IGQHIKLPQF YIYDEEGGYD VSKPVMISQW PISNDSNGCV 1000
VEASTDFHQL ECIVDDSVRE EVDIIEQPFE EVEHVLSIKQ PFSFSFRDEL 1050
GVRVLDQSDN NCWISTTLVQ LQLTKLLDDS IEMQLFKVGK VDSIVQKCYE 1100
LSHLISGSLG DSGKLLSELL KEKYTCSITF EMSCDCGKKF DDQVGCLFWI 1150
MPYTKLFQKG ECCICHKMQT YKLVSMKGTG VFVQDPAPID IDAFPVKPIC 1200
SSVYLGVKGS GHYQTNLYSF NKAIDGFGVF DIKNSSVNTV CFVDVDFHSV 1250
EIEAGEVKPF AVYKNVKFYL GDISHLVNCV SFDFVVNAAN ENLLHGGGVA 1300
RAIDILTEGQ LQSLSKDYIS SNGPLKVGAG VMLECEKFNV FNVVGPRTGK 1350
HEHSLLVEAY NSILFENGIP LMPLLSCGIF GVRIENSLKA LFSCDINKPL 1400
QVFVYSSNEE QAVLKFLDGL DLTPVIDDVD VVKPFRVEGN FSFFDCGVNA 1450
LDGDIYLLFT NSILMLDKQG QLLDTKLNGI LQQAALDYLA TVKTVPAGNL 1500
VKLFVESCTI YMCVVPSIND LSFDKNLGRC VRKLNRLKTC VIANVPAIDV 1550
LKKLLSSLTL TVKFVVESNV MDVNDCFKND NVVLKITEDG INVKDVVVES 1600
SKSLGKQLGV VSDGVDSFEG VLPINTDTVL SVAPEVDWVA FYGFEKAALF 1650
ASLDVKPYGY PNDFVGGFRV LGTTDNNCWV NATCIILQYL KPTFKSKGLN 1700
VLWNKFVTGD VGPFVSFIYF ITMSSKGQKG DAEEALSKLS EYLISDSIVT 1750
LEQYSTCDIC KSTVVEVKSA IVCASVLKDG CDVGFCPHRH KLRSRVKFVN 1800
GRVVITNVGE PIISQPSKLL NGIAYTTFSG SFDNGHYVVY DAANNAVYDG 1850
ARLFSSDLST LAVTAIVVVG GCVTSNVPTI VSEKISVMDK LDTGAQKFFQ 1900
FGDFVMNNIV LFLTWLLSMF SLLRTSIMKH DIKVIAKAPK RTGVILTRSF 1950
KYNIRSALFV IKQKWCVIVT LFKFLLLLYA IYALVFMIVQ FSPFNSLLCG 2000
DIVSGYEKST FNKDIYCGNS MVCKMCLFSY QEFNDLDHTS LVWKHIRDPI 2050
LISLQPFVIL VILLIFGNMY LRFGLLYFVA QFISTFGSFL GFHQKQWFLH 2100
FVPFDVLCNE FLATFIVCKI VLFVRHIIVG CNNADCVACS KSARLKRVPL 2150
QTIINGMHKS FYVNANGGTC FCNKHNFFCV NCDSFGPGNT FINGDIAREL 2200
GNVVKTAVQP TAPAYVIIDK VDFVNGFYRL YSGDTFWRYD FDITESKYSC 2250
KEVLKNCNVL ENFIVYNNSG SNITQIKNAC VYFSQLLCEP IKLVNSELLS 2300
TLSVDFNGVL HKAYVDVLCN SFFKELTANM SMAECKATLG LTVSDDDFVS 2350
AVANAHRYDV LLSDLSFNNF FISYAKPEDK LSVYDIACCM RAGSKVVNHN 2400
VLIKESIPIV WGVKDFNTLS QEGKKYLVKT TKAKGLTFLL TFNDNQAITQ 2450
VPATSIVAKQ GAGFKRTYNF LWYVCLFVVA LFIGVSFIDY TTTVTSFHGY 2500
DFKYIENGQL KVFEAPLHCV RNVFDNFNQW HEAKFGVVTT NSDKCPIVVG 2550
VSERINVVPG VPTNVYLVGK TLVFTLQAAF GNTGVCYDFD GVTTSDKCIF 2600
NSACTRLEGL GGDNVYCYNT DLIEGSKPYS TLQPNAYYKY DAKNYVRFPE 2650
ILARGFGLRT IRTLATRYCR VGECRDSHKG VCFGFDKWYV NDGRVDDGYI 2700
CGDGLIDLLV NVLSIFSSSF SVVAMSGHML FNFLFAAFIT FLCFLVTKFK 2750
RVFGDLSYGV FTVVCATLIN NISYVVTQNL FFMLLYAILY FVFTRTVRYA 2800
WIWHIAYIVA YFLLIPWWLL TWFSFAAFLE LLPNVFKLKI STQLFEGDKF 2850
IGTFESAAAG TFVLDMRSYE RLINTISPEK LKNYAASYNK YKYYSGSASE 2900
ADYRCACYAH LAKAMLDYAK DHNDMLYSPP TISYNSTLQS GLKKMAQPSG 2950
CVERCVVRVC YGSTVLNGVW LGDTVTCPRH VIAPSTTVLI DYDHAYSTMR 3000
LHNFSVSHNG VFLGVVGVTM HGSVLRIKVS QSNVHTPKHV FKTLKPGDSF 3050
NILACYEGIA SGVFGVNLRT NFTIKGSFIN GACGSPGYNV RNDGTVEFCY 3100
LHQIELGSGA HVGSDFTGSV YGNFDDQPSL QVESANLMLS DNVVAFLYAA 3150
LLNGCRWWLC STRVNVDGFN EWAMANGYTS VSSVECYSIL AAKTGVSVEQ 3200
LLASIQHLHE GFGGKNILGY SSLCDEFTLA EVVKQMYGVN LQSGKVIFGL 3250
KTMFLFSVFF TMFWAELFIY TNTIWINPVI LTPIFCLLLF LSLVLTMFLK 3300
HKFLFLQVFL LPTVIATALY NCVLDYYIVK FLADHFNYNV SVLQMDVQGL 3350
VNVLVCLFVV FLHTWRFSKE RFTHWFTYVC SLIAVAYTYF YSGDFLSLLV 3400
MFLCAISSDW YIGAIVFRLS RLIVFFSPES VFSVFGDVKL TLVVYLICGY 3450
LVCTYWGILY WFNRFFKCTM GVYDFKVSAA EFKYMVANGL HAPHGPFDAL 3500
WLSFKLLGIG GDRCIKISTV QSKLTDLKCT NVVLLGCLSS MNIAANSSEW 3550
AYCVDLHNKI NLCDDPEKAQ SMLLALLAFF LSKHSDFGLD GLIDSYFDNS 3600
STLQSVASSF VSMPSYIAYE NARQAYEDAI ANGSSSQLIK QLKRAMNIAK 3650
SEFDHEISVQ KKINRMAEQA ATQMYKEARS VNRKSKVISA MHSLLFGMLR 3700
RLDMSSVETV LNLARDGVVP LSVIPATSAS KLTIVSPDLE SYSKIVCDGS 3750
VHYAGVVWTL NDVKDNDGRP VHVKEITKEN VETLTWPLIL NCERVVKLQN 3800
NEIMPGKLKQ KPMKAEGDGG VLGDGNALYN TEGGKTFMYA YISNKADLKF 3850
VKWEYEGGCN TIELDSPCRF MVETPNGPQV KYLYFVKNLN TLRRGAVLGF 3900
IGATIRLQAG KQTELAVNSG LLTACAFSVD PATTYLEAVK HGAKPVSNCI 3950
KMLSNGAGNG QAITTSVDAN TNQDSYGGAS ICLYCRAHVP HPSMDGYCKF 4000
KGKCVQVPIG CLDPIRFCLE NNVCNVCGCW LGHGCACDRT TIQSVDISYL 4050
NRARGSSAAR LEPCNGTDID KCVRAFDIYN KNVSFLGKCL KMNCVRFKNA 4100
DLKDGYFVIK RCTKSVMEHE QSMYNLLNFS GALAEHDFFT WKDGRVIYGN 4150
VSRHNLTKYT MMDLVYAMRN FDEQNCDVLK EVLVLTGCCD NSYFDSKGWY 4200
DPVENEDIHR VYASLGKIVA RAMLKCVALC DAMVAKGVVG VLTLDNQDLN 4250
GNFYDFGDFV VSLPNMGVPC CTSYYSYMMP IMGLTNCLAS ECFVKSDIFG 4300
SDFKTFDLLK YDFTEHKENL FNKYFKHWSF DYHPNCSDCY DDMCVIHCAN 4350
FNTLFATTIP GTAFGPLCRK VFIDGVPLVT TAGYHFKQLG LVWNKDVNTH 4400
SVRLTITELL QFVTDPSLII ASSPALVDQR TICFSVAALS TGLTNQVVKP 4450
GHFNEEFYNF LRLRGFFDEG SELTLKHFFF AQNGDAAVKD FDFYRYNKPT 4500
ILDICQARVT YKIVSRYFDI YEGGCIKACE VVVTNLNKSA GWPLNKFGKA 4550
SLYYESISYE EQDALFALTK RNVLPTMTQL NLKYAISGKE RARTVGGVSL 4600
LSTMTTRQYH QKHLKSIVNT RNATVVIGTT KFYGGWNNML RTLIDGVENP 4650
MLMGWDYPKC DRALPNMIRM ISAMVLGSKH VNCCTATDRF YRLGNELAQV 4700
LTEVVYSNGG FYFKPGGTTS GDASTAYANS IFNIFQAVSS NINRLLSVPS 4750
DSCNNVNVRD LQRRLYDNCY RLTSVEESFI DDYYGYLRKH FSMMILSDDG 4800
VVCYNKDYAE LGYIADISAF KATLYYQNNV FMSTSKCWVE EDLTKGPHEF 4850
CSQHTMQIVD KDGTYYLPYP DPSRILSAGV FVDDVVKTDA VVLLERYVSL 4900
AIDAYPLSKH PNSEYRKVFY VLLDWVKHLN KNLNEGVLES FSVTLLDNQE 4950
DKFWCEDFYA SMYENSTILQ AAGLCVVCGS QTVLRCGDCL RKPMLCTKCA 5000
YDHVFGTDHK FILAITPYVC NASGCGVSDV KKLYLGGLNY YCTNHKPQLS 5050
FPLCSAGNIF GLYKNSATGS LDVEVFNRLA TSDWTDVRDY KLANDVKDTL 5100
RLFAAETIKA KEESVKSSYA FATLKEVVGP KELLLSWESG KVKPPLNRNS 5150
VFTCFQISKD SKFQIGEFIF EKVEYGSDTV TYKSTVTTKL VPGMIFVLTS 5200
HNVQPLRAPT IANQEKYSSI YKLHPAFNVS DAYANLVPYY QLIGKQKITT 5250
IQGPPGSGKS HCSIGLGLYY PGARIVFVAC AHAAVDSLCA KAMTVYSIDK 5300
CTRIIPARAR VECYSGFKPN NTSAQYIFST VNALPECNAD IVVVDEVSMC 5350
TNYDLSVINQ RLSYKHIVYV GDPQQLPAPR VMITKGVMEP VDYNVVTQRM 5400
CAIGPDVFLH KCYRCPAEIV NTVSELVYEN KFVPVKPASK QCFKVFFKGN 5450
VQVDNGSSIN RKQLEIVKLF LVKNPSWSKA VFISPYNSQN YVASRFLGLQ 5500
IQTVDSSQGS EYDYVIYAQT SDTAHACNVN RFNVAITRAK KGIFCVMCDK 5550
TLFDSLKFFE IKHADLHSSQ VCGLFKNCTR TPLNLPPTHA HTFLSLSDQF 5600
KTTGDLAVQI GSNNVCTYEH VISFMGFRFD ISIPGSHSLF CTRDFAIRNV 5650
RGWLGMDVES AHVCGDNIGT NVPLQVGFSN GVNFVVQTEG CVSTNFGDVI 5700
KPVCAKSPPG EQFRHLIPLL RKGQPWLIVR RRIVQMISDY LSNLSDILVF 5750
VLWAGSLELT TMRYFVKIGP IKYCYCGNSA TCYNSVSNEY CCFKHALGCD 5800
YVYNPYAFDI QQWGYVGSLS QNHHTFCNIH RNEHDASGDA VMTRCLAVHD 5850
CFVKNVDWTV TYPFIANEKF INGCGRNVQG HVVRAALKLY KPSVIHDIGN 5900
PKGVRCAVTD AKWYCYDKQP VNSNVKLLDY DYATHGQLDG LCLFWNCNVD 5950
MYPEFSIVCR FDTRTRSVFN LEGVNGGSLY VNKHAFHTPA YDKRAFVKLK 6000
PMPFFYFDDS DCDVVQEQVN YVPLRASSCV TRCNIGGAVC SKHANLYQKY 6050
VEAYNTFTQA GFNIWVPHSF DVYNLWQIFI ETNLQSLENI AFNVVKKGCF 6100
TGVDGELPVA VVNDKVFVRY GDVDNLVFTN KTTLPTNVAF ELFAKRKMGL 6150
TPPLSILKNL GVVATYKFVL WDYEAERPFT SYTKSVCKYT DFNEDVCVCF 6200
DNSIQGSYER FTLTTNAVLF STVVIKNLTP IKLNFGMLNG MPVSSIKGDK 6250
GVEKLVNWYI YVRKNGQFQD HYDGFYTQGR NLSDFTPRSD MEYDFLNMDM 6300
GVFINKYGLE DFNFEHVVYG DVSKTTLGGL HLLISQFRLS KMGVLKADDF 6350
VTASDTTLRC CTVTYLNELS SKVVCTYMDL LLDDFVTILK SLDLGVISKV 6400
HEVIIDNKPY RWMLWCKDNH LSTFYPQLQS AEWKCGYAMP QIYKLQRMCL 6450
EPCNLYNYGA GIKLPSGIML NVVKYTQLCQ YLNSTTMCVP HNMRVLHYGA 6500
GSDKGVAPGT TVLKRWLPPD AIIIDNDIND YVSDADFSIT GDCATVYLED 6550
KFDLLISDMY DGRIKFCDGE NVSKDGFFTY LNGVIREKLA IGGSVAIKIT 6600
EYSWNKYLYE LIQRFAFWTL FCTSVNTSSS EAFLIGINYL GDFIQGPFIA 6650
GNTVHANYIF WRNSTIMSLS YNSVLDLSKF ECKHKATVVV TLKDSDVNDM 6700
VLSLIKSGRL LLRNNGRFGG FSNHLVSTK 6729

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:6,729
Mass (Da):752,702
Last modified:June 10, 2008 - v1
Checksum:i86F9F04C687A65FA
GO
Isoform Replicase polyprotein 1a (identifier: P0C6U6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6U6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,060
Mass (Da):451,388
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY567487 Genomic RNA. Translation: AAS58176.2.
RefSeqiYP_003766.2. NC_005831.2. [P0C6X5-1]

Genome annotation databases

GeneIDi2943501.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY567487 Genomic RNA. Translation: AAS58176.2 .
RefSeqi YP_003766.2. NC_005831.2. [P0C6X5-1 ]

3D structure databases

ProteinModelPortali P0C6X5.
SMRi P0C6X5. Positions 2940-3240, 3802-3907, 3915-4039.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2943501.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR011050. Pectin_lyase_fold/virulence.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view ]
SMARTi SM00506. A1pp. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF51126. SSF51126. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Amsterdam I.
  2. "Deubiquitinating and interferon antagonism activities of coronavirus papain-like proteases."
    Clementz M.A., Chen Z., Banach B.S., Wang Y., Sun L., Ratia K., Baez-Santos Y.M., Wang J., Takayama J., Ghosh A.K., Li K., Mesecar A.D., Baker S.C.
    J. Virol. 84:4619-4629(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiR1AB_CVHNL
AccessioniPrimary (citable) accession number: P0C6X5
Secondary accession number(s): Q6Q1S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi