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P0C6X0 (R1AB_CVBQ) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p28
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PL1/PL2
    Papain-like proteinases 1/2
    p210
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
    p44
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p27
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p10
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p22
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p15
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
    p100
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
    p67
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
    p35
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismBovine coronavirus (strain Quebec) (BCoV) (BCV) [Complete proteome]
Taxonomic identifier11133 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length7059 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Sequence caution

The sequence AAL40396.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAL40397.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Eukaryotic host gene expression shutoff by virus
Eukaryotic host translation shutoff by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6X0-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6U1-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6U1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Non-structural protein 1 By similarity
PRO_0000037274
Chain247 – 851605Non-structural protein 2 By similarity
PRO_0000037275
Chain852 – 27501899Non-structural protein 3 By similarity
PRO_0000037276
Chain2751 – 3246496Non-structural protein 4 By similarity
PRO_0000037277
Chain3247 – 35493033C-like proteinase By similarity
PRO_0000037278
Chain3550 – 3836287Non-structural protein 6 By similarity
PRO_0000037279
Chain3837 – 392589Non-structural protein 7 By similarity
PRO_0000037280
Chain3926 – 4122197Non-structural protein 8 By similarity
PRO_0000037281
Chain4123 – 4232110Non-structural protein 9 By similarity
PRO_0000037282
Chain4233 – 4369137Non-structural protein 10 By similarity
PRO_0000037283
Chain4370 – 5297928RNA-directed RNA polymerase By similarity
PRO_0000037284
Chain5298 – 5900603Helicase By similarity
PRO_0000037285
Chain5901 – 6421521Exoribonuclease By similarity
PRO_0000037286
Chain6422 – 6795374Uridylate-specific endoribonuclease By similarity
PRO_0000037287
Chain6796 – 7059264Putative 2'-O-methyl transferase By similarity
PRO_0000037288

Regions

Transmembrane2138 – 215821Helical; Potential
Transmembrane2199 – 221921Helical; Potential
Transmembrane2221 – 224121Helical; Potential
Transmembrane2313 – 233321Helical; Potential
Transmembrane2343 – 236321Helical; Potential
Transmembrane2365 – 238521Helical; Potential
Transmembrane2752 – 277221Helical; Potential
Transmembrane2824 – 284421Helical; Potential
Transmembrane3009 – 302921Helical; Potential
Transmembrane3031 – 305121Helical; Potential
Transmembrane3063 – 308321Helical; Potential
Transmembrane3090 – 311021Helical; Potential
Transmembrane3115 – 313521Helical; Potential
Transmembrane3558 – 357821Helical; Potential
Transmembrane3588 – 360821Helical; Potential
Transmembrane3615 – 363521Helical; Potential
Transmembrane3657 – 367721Helical; Potential
Transmembrane3684 – 370421Helical; Potential
Transmembrane3711 – 373121Helical; Potential
Transmembrane3755 – 377521Helical; Potential
Domain1036 – 1274239Peptidase C16 1
Domain1275 – 1435161Macro
Domain1631 – 1892262Peptidase C16 2
Domain3247 – 3549303Peptidase C30
Domain4977 – 5139163RdRp catalytic
Domain5298 – 538184CV MBD
Domain5553 – 5734182(+)RNA virus helicase ATP-binding
Domain5735 – 5904170(+)RNA virus helicase C-terminal
Zinc finger1151 – 117929C4-type 1
Zinc finger1749 – 178537C4-type 2
Zinc finger4306 – 432217 By similarity
Zinc finger4348 – 436114 By similarity
Nucleotide binding5578 – 55858ATP By similarity
Region2138 – 2385248HD1
Region2752 – 3135384HD2
Region3319 – 3775457HD3

Sites

Active site10741For PL1-PRO activity By similarity
Active site12251For PL1-PRO activity By similarity
Active site16711For PL2-PRO activity By similarity
Active site18281For PL2-PRO activity By similarity
Active site32871For 3CL-PRO activity By similarity
Active site33911For 3CL-PRO activity By similarity
Site246 – 2472Cleavage; by PL1-PRO By similarity
Site851 – 8522Cleavage; by PL1-PRO By similarity
Site2750 – 27512Cleavage; by PL2-PRO By similarity
Site3246 – 32472Cleavage; by 3CL-PRO By similarity
Site3549 – 35502Cleavage; by 3CL-PRO By similarity
Site3836 – 38372Cleavage; by 3CL-PRO By similarity
Site3925 – 39262Cleavage; by 3CL-PRO By similarity
Site4122 – 41232Cleavage; by 3CL-PRO By similarity
Site4232 – 42332Cleavage; by 3CL-PRO By similarity
Site4369 – 43702Cleavage; by 3CL-PRO By similarity
Site5297 – 52982Cleavage; by 3CL-PRO By similarity
Site5900 – 59012Cleavage; by 3CL-PRO By similarity
Site6421 – 64222Cleavage; by 3CL-PRO By similarity
Site6795 – 67962Cleavage; by 3CL-PRO By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 98721241C296B7D6

FASTA7,059792,766
        10         20         30         40         50         60 
MSKINKYGLE LHWAPEFPWM FEDAEEKLDN PSSSEVDIVC STTAQKLETG GICPENHVMV 

        70         80         90        100        110        120 
DCRRLLKQEC CVQSSLIREI VMNTRPYDLE VLLQDALQSR EAVLVTPPLG MSLEACYVRG 

       130        140        150        160        170        180 
CNPNGWTMGL FRRRSVCNTG RCAVNKHVAY QLYMIDPAGV CFGAGQFVGW VIPLAFMPVQ 

       190        200        210        220        230        240 
SRKFIAPWVM YLRKCGEKGA YIKDYKRGGF EHVYNFKVED AYDLVHDEPK GKFSKKAYAL 

       250        260        270        280        290        300 
IRGYRGVKPL LYVDQYGCDY TGGLADGLEA YADKTLQEMK ALFPIWSQEL PFDVTVAWHV 

       310        320        330        340        350        360 
VRDPRYVMRL QSASTIRSVA YVANPTEDLC DGSVVIKEPV HVYADDSIIL RQHNLVDIMS 

       370        380        390        400        410        420 
CFYMEADAVV NAFYGVDLKD CGFVMQFGYI DCEQDLCDFK GWVPGNMIDG FACTTCGHVY 

       430        440        450        460        470        480 
ETGDLLAQSS GVLPVNPVLH TKSAAGYGGF GCKDSFTLYG QTVVYFGGCV YWSPARNIWI 

       490        500        510        520        530        540 
PILKSSVKSY DGLVYTGVVG CKAIVKETNL ICKALYLDYV QHKCGNLHQR ELLGVSDVWH 

       550        560        570        580        590        600 
KQLLLNRGVY KPLLENIDYF NMRRAKFSLE TFTVCADGFM PFLLDDLVPR AYYLAVSGQA 

       610        620        630        640        650        660 
FCDYAGKICH AVVSKSKELL DVSVDSLGAA IHYLNSKIVD LAQHFSDFGT SFVSKIVHFF 

       670        680        690        700        710        720 
KTFTTSTALA FAWVLFHVLH GAYIVVESDI YFGKNIPRYA SAVAQAFRSG AKVGLDSLRV 

       730        740        750        760        770        780 
TFIDGLSCFK IGRRRICLSG SKIYEVERGL LHSSQLPLDV YDLTMPSQVQ KTKQKGIYLK 

       790        800        810        820        830        840 
GSGSDFSLAD SVVEVVTTSL TPCGYSEPPK VADKICIVDN VYMAKAGDKY YPVVVDGHVG 

       850        860        870        880        890        900 
LLDQAWRVPC AGRCVTFKEQ PTVNEIASTP KTIKVFYELD KDFNTILNTA CGEFEVDDTV 

       910        920        930        940        950        960 
DMEEFYAVVI DAIEEKLSPC KELEGVGAKV SAFLQKLEDN SLFLFDEAGE EVLAPKLYCA 

       970        980        990       1000       1010       1020 
FTAPEDDDFL EESGVEEDDV EGEETDLTVT SAGEPCVASE QEESSEILED TLDDGPCVET 

      1030       1040       1050       1060       1070       1080 
SDSQVEEDVQ MSDFGDLESV IQDYENVCFE FYTTEPEFVK VLDLYVPKAT RNNCWLRSVL 

      1090       1100       1110       1120       1130       1140 
AVMQKLPCQF KDKNLQDLWV LYKQQYSQLF VDTLVNKIPA NIVVPQGGYV ADFAYWFLTL 

      1150       1160       1170       1180       1190       1200 
CDWQCVAYWK CIKCDLALKL KGLDAMFFYG DVVSHVCKCG ESMVLIDVDV PFTAHFALKD 

      1210       1220       1230       1240       1250       1260 
KLFCAFITKR SVYKAACVVD VNDSHSMAVV DGKQIDDHRI TSITSDKFDF IIGHGTSFSM 

      1270       1280       1290       1300       1310       1320 
TTFEIAQLYG SCITPNVCFV KGDIIKVSKR VKAEVVVNPA NGHMAHGGGV AKAIAVAAGQ 

      1330       1340       1350       1360       1370       1380 
QFVKETTDMV KSKGVCATGD CYVSTGGKLC KTVLNVVGPD ARTQGKQSYA LLERVYKHLN 

      1390       1400       1410       1420       1430       1440 
KYDCVVTTLI SAGIFSVPSD VSLTYLLGTA KKQVVLVSNN QEDFDLISKC QITAVEGTKK 

      1450       1460       1470       1480       1490       1500 
LAERLSFNVG RSIVYETDAN KLILSNDVAF VSTFNVLQDV LSLRHDIALD DDARTFVQSN 

      1510       1520       1530       1540       1550       1560 
VDVVPEGWRV VNKFYQINGV RPVKYFECPG GIDICSQDKV FGYVQQGSFN KATVAQIKAL 

      1570       1580       1590       1600       1610       1620 
FLDKVDILLT VDGVNFTNRF VPVGESFGKS LGNVFCDGVN VTKHKCDINY KGKVFFQFDN 

      1630       1640       1650       1660       1670       1680 
LSSEDLKAVR SSFNFDQKEL LAYYNMLVNC SKWQVVFNGK YFTFKQANNN CFVNVSCLML 

      1690       1700       1710       1720       1730       1740 
QSLNLKFKIV QWQEAWLEFR SGRPARFVSL VLAKGGFKFG DPADSRDFLR VVFSQVDLTG 

      1750       1760       1770       1780       1790       1800 
AICDFEIACK CGVKQEQRTG VDAVMHFGTL SREDLEIGYT VDCSCGKKLI HCVRFDVPFL 

      1810       1820       1830       1840       1850       1860 
ICSNTPASVK LPKGVGSANI FKGDKVGHYV HVKCEQSYQL YDASNVKKVT DVTGNLSDCL 

      1870       1880       1890       1900       1910       1920 
YLKNLKQTFK SVLTTYYLDD VKKIEYKPDL SQYYCDGGKY YTQRIIKAQF KTFEKVDGVY 

      1930       1940       1950       1960       1970       1980 
TNFKLIGHTV CDILNAKLGF DSSKEFVEYK VTEWPTATGD VVLATDDLYV KRYERGCITF 

      1990       2000       2010       2020       2030       2040 
GKPVIWLSHE QASLNSLTYF NRPLLVDENK FDVLKVDDVD DGGDISESDA KEPKEINIIK 

      2050       2060       2070       2080       2090       2100 
LSGVKKPFKV EDSVIVNDDT SEIKYVKSLS IVDVYDMWLT GCRCVVRTAN ALSRAVNVPT 

      2110       2120       2130       2140       2150       2160 
IRKFIKFGMT LVSIPIDLLN LREIKPVFNV VKAVRNKISA CFNFIKWLFV LLFGWIKISA 

      2170       2180       2190       2200       2210       2220 
DNKVIYTTEV ASKLTCKLVA LAFKNAFLTF KWSVVARGAC IIATIFLLWF NFIYANVIFS 

      2230       2240       2250       2260       2270       2280 
DFYLPKIGFL PTFVGKIVQW IKNTFSLVTI CDLYSIQDVG FKNQYCNGSI ACQFCLAGFD 

      2290       2300       2310       2320       2330       2340 
MLDNYKAIDV VQYEADRRAF VDYTGVLKIV IELIVSYALY TAWFYPLFAL ISIQILTTWL 

      2350       2360       2370       2380       2390       2400 
PELLMLSTLH WSVRLLVSLA NMLPAHVFMR FYIIIASFIK LFSLFRHVAY GCSKSGCLFC 

      2410       2420       2430       2440       2450       2460 
YKRNRSLRVK CSTIVGGMIR YYDVMANGGT GFCSKHQWNC IDCDSYKPGN TFITVEAALD 

      2470       2480       2490       2500       2510       2520 
LSKELKRPIQ PTDVAYHTVT DVKQVGCYMR LFYDRDGQRT YDDVNASLFV DYSNLLHSKV 

      2530       2540       2550       2560       2570       2580 
KSVPNMHVVV VENDADKANF LNAAVFYAQS LFRPILMVDK ILITTANTGT SVTETMFDVY 

      2590       2600       2610       2620       2630       2640 
VDTFLSMFDV DKKSLNALIA TAHSSIKQGT QICKVLDTFL SCARKSCSID SDVDTKCLAD 

      2650       2660       2670       2680       2690       2700 
SVMSAVSAGL ELTDESCNNL VPTYLKGDNI VAADLGVLIQ NSAKHVQGNV AKIAGVSCIW 

      2710       2720       2730       2740       2750       2760 
SVDAFNQLSS DFQHKLKKAC CKTGLKLELT YNKQMANVSV LTTPFSLKGG AVFSYFVYVC 

      2770       2780       2790       2800       2810       2820 
FVLSLVCFIG LWCLMPTYTV HKSDFQLPVY ASYKVLDNGV IRDVSVEDVC FANKFEQFDQ 

      2830       2840       2850       2860       2870       2880 
WYESTFGLSY YSNSMACPIV VAVVDQDFGS TVFNVPTKVL RYGYHVLHFI THALSADGVQ 

      2890       2900       2910       2920       2930       2940 
CYTPHSQISY SNFYASGCVL SSACTMFAMA DGSPQPYCYT DGLMQNASLY SSLVPHVRYN 

      2950       2960       2970       2980       2990       3000 
LANAKGFIRL PEVLREGLVR IVRTRSMSYC RVGLCEEADE GICFNFNGSW VLNNDYYRSL 

      3010       3020       3030       3040       3050       3060 
PGTFCGRDVF DLIYQLFKGL AQPVDFLALT ASSIAGAILA VIVVLGFYYL IKLKRAFGDY 

      3070       3080       3090       3100       3110       3120 
TSIVFVNVIV WCVNFMMLFV FQVYPTLSCV YAICYFYATL YFPSEISVIM HLQWLVMYGT 

      3130       3140       3150       3160       3170       3180 
IMPLWFCLLY ISVVVSNHAF WVFSYCRQLG TSVRSDGTFE EMALTTFMIT KDSYCKLKNS 

      3190       3200       3210       3220       3230       3240 
LSDVAFNRYL SLYNKYRYYS GKMDTAAYRE AACSQLAKAM DTFTNNNGSD VLYQPPTASV 

      3250       3260       3270       3280       3290       3300 
STSFLQSGIV KMVNPTSKVE PCIVSVTYGN MTLNGLWLDD KVYCPRHVIC SASDMTNPDY 

      3310       3320       3330       3340       3350       3360 
TNLLCRVTSS DFTVLFDRLS LTVMSYQMQG CMLVLTVTLQ NSRTPKYTFG VVKPGETFTV 

      3370       3380       3390       3400       3410       3420 
LAAYNGKPQG AFHVTMRSSY TIKGSFLCGS CGSVGYVIMG DCVKFVYMHQ LELSTGCHTG 

      3430       3440       3450       3460       3470       3480 
TDFNGDFYGP YKDAQVVQLP VQDYIQSVNF VAWLYAAILN NCNWFVQSDK CSVEDFNVWA 

      3490       3500       3510       3520       3530       3540 
LSNGFSQVKS DLVIDALASM TGVSLETLLA AIKRLKNGFQ GRQIMGSCSF EDELTPSDVY 

      3550       3560       3570       3580       3590       3600 
QQLAGIKLQS KRTRLVKGIV CWIMASTFLF SCIITAFVKW TMFMYVTTNM LSITFCALCV 

      3610       3620       3630       3640       3650       3660 
ISLAMLLVKH KHLYLTMYII PVLFTLLYNN YLVVYKQTFR GYVYAWLSYY VPSVEYTYTD 

      3670       3680       3690       3700       3710       3720 
EVIYGMLLLI GMVFVTLRSI NHDLFSFIMF VGRVISVVSL WYMGSNLEEE ILLMLASLFG 

      3730       3740       3750       3760       3770       3780 
TYTWTTALSM AAAKVIAKWV AVNVLYFTDI PQIKIVLVCY LFIGYIISCY WGLFSLMNSL 

      3790       3800       3810       3820       3830       3840 
FRMPLGVYNY KISVQELRYM NANGLRPPKN SFEALMLNFK LLGIGGVPII EVSQFQSKLT 

      3850       3860       3870       3880       3890       3900 
DVKCANGGLL NCLQHLHVAS NSKLWQYCST LHNEILATSD LGVAFEKLAQ LLIVLFANPA 

      3910       3920       3930       3940       3950       3960 
AVDSKCLTSI EEVCDDYAKD NTVLQALQSE FVNMASFVEY EVAKKNLDEA CSSGSANQQQ 

      3970       3980       3990       4000       4010       4020 
LKQLEKACNI AKSAYERDRA VARKLERMAD LALTNMYKEA RINDKKSKVV SALQTMLFSM 

      4030       4040       4050       4060       4070       4080 
VRKLDNQALN SILDNAVKGC VPLNAIPSLA ANTLTIIVPD KSVYDQVVDN VYVTYAGNVW 

      4090       4100       4110       4120       4130       4140 
QIQTIQDSDG TNKQLHEISD DCNWPLVIIA NRHNEVSATV LQNNELMPAK LKTQVVNSGP 

      4150       4160       4170       4180       4190       4200 
DQTCNTPTQC YYNNSYNGKI VYAILSDVDG LKYTKILKDD GNFVVLELDP PCKFTVQDVK 

      4210       4220       4230       4240       4250       4260 
GLKIKYLYFV KGCNTLARGW VVGTISSTVR LQAGTATEYA SNSSILSLCA FSVDPKKTYL 

      4270       4280       4290       4300       4310       4320 
DFIQQGGTPI ANCVKMLCDH AGTGMAITVK PDATTSQDSY GGASVCIYCR ARVEHPDVDG 

      4330       4340       4350       4360       4370       4380 
LCKLRGKFVQ VPVGIKDPVS YVLTHDVCQV CGFWRDGSCS CVSTDTTVQS KDTNFLNRVR 

      4390       4400       4410       4420       4430       4440 
GTSVDARLVP CASGLSTDVQ LRAFDICNAS VAGIGLHLKV NCCRFQRVDE NGDKLDQFFV 

      4450       4460       4470       4480       4490       4500 
VKRTDLTIYN REMECYERVK DCKFVAEHDF FTFDVEGSRV PHIVRKDLTK YTMLDLCYAL 

      4510       4520       4530       4540       4550       4560 
RHFDRNDCML LCDILSIYAG CEQSYFTKKD WYDFVENPDI INVYKKLGPI FNRALVSATE 

      4570       4580       4590       4600       4610       4620 
FADKLVEVGL VGILTLDNQD LNGKWYDFGD YVIAAPGCGV AIADSYYSYM MPMLTMCHAL 

      4630       4640       4650       4660       4670       4680 
DCELYVNNAY RLFDLVQYDF TDYKLELFNK YFKHWSMPYH PNTVDCQDDR CIIHCANFNI 

      4690       4700       4710       4720       4730       4740 
LFSMVLPNTC FGPLVRQIFV DGVPFVVSIG YHYKELGIVM NMDVDTHRYR LSLKDLLLYA 

      4750       4760       4770       4780       4790       4800 
ADPALHVASA SALYDLRTCC FSVAAITSGV KFQTVKPGNF NQDFYDFILS KGLLKEGSSV 

      4810       4820       4830       4840       4850       4860 
DLKHFFFTQD GNAAITDYNY YKYNLPTMVD IKQLLFVLEV VYKYFEIYDG GCIPAAQVIV 

      4870       4880       4890       4900       4910       4920 
NNYDKSAGYP FNKFGKARLY YEALSFEEQD EIYAYTKRNV LPTLTQMNLK YAISAKNRAR 

      4930       4940       4950       4960       4970       4980 
TVAGVSILST MTGRMFHQKC LKSIAATRGV PVVIGTTKFY GGWDDMLRRL IKDVDNPVLM 

      4990       5000       5010       5020       5030       5040 
GWDYPKCDRA MPNILRIVSS LVLARKHEAC CSQSDRFYRL ANECAQVLSE IVMCGGCYYV 

      5050       5060       5070       5080       5090       5100 
KPGGTSSGDA TTAFANSVFN ICQAVSANVC ALMSCNGNKI EDLSIRALQK RLYSHVYRSD 

      5110       5120       5130       5140       5150       5160 
MVDSTFVTEY YEFLNKHFSM MILSDDGVVC YNSDYASKGY IANISAFQQV LYYQNNVFMS 

      5170       5180       5190       5200       5210       5220 
ESKCWVENDI NNGPHEFCSQ HTMLVKMDGD DVYLPYPVPS RILGAGCFVD DLLKTDSVLL 

      5230       5240       5250       5260       5270       5280 
IERFVSLAID AYPLVYHENE EYQKVFRVYL EYIKKLYNEL GNQILDSYSV ILSTCDGQKF 

      5290       5300       5310       5320       5330       5340 
TDESFYKNMY LRSAVMQSVG ACVVCSSQTS LRCGSCIRKP LLCCKCCYDH VMATDHKYVL 

      5350       5360       5370       5380       5390       5400 
SVSPYVCNAP GCDVNDVTKL YLGGMSYYCE DHKPQYSFKL VMNGMVFGLY KQSCTGSPYI 

      5410       5420       5430       5440       5450       5460 
DDFNRIASCK WTDVDDYILA NECTERLKLF AAETQKATEE AFKQSYASAT IQEIVSEREL 

      5470       5480       5490       5500       5510       5520 
ILSWEIGKVK PPLNKNYVFT GYHFTKNGKT VLGEYVFDKS ELTNGVYYRA TTTYKLSVGD 

      5530       5540       5550       5560       5570       5580 
VFVLTSHSVA NLSAPTLVPQ ENYSSIRFAS VYSVLETFQN NVVNYQHIGM KRYCTVQGPP 

      5590       5600       5610       5620       5630       5640 
GTGKSHLAIG LAVYYCTARV VYTAASHAAV DALCEKAYKF LNINDCTRIV PAKVRVECYD 

      5650       5660       5670       5680       5690       5700 
KFKINDTTRK YVFTTINALP EMVTDIVVVD EVSMLTNYEL SVINARIRAK HYVYIGDPAQ 

      5710       5720       5730       5740       5750       5760 
LPAPRVLLSK GTLEPKYFNT VTKLMCCLGP DIFLGTCYRC PKEIVDTVSA LVYENKLKAK 

      5770       5780       5790       5800       5810       5820 
NESSSLCFKV YYKGVTTHES SSAVNMQQIY LINKFLKANP LWHKAVFISP YNSQNFAAKR 

      5830       5840       5850       5860       5870       5880 
VLGLQTQTVD SAQGSEYDYV IYSQTAETAH SVNVNRFNVA ITRAKKGILC VMSNMQLFEA 

      5890       5900       5910       5920       5930       5940 
LQFTTLTVDK VPQAVETRVQ CSTNLFKDCS KSYSGYHPAH APSFLAVDDK YKATGDLAVC 

      5950       5960       5970       5980       5990       6000 
LGIGDSAVTY SRLISLMGFK LDVTLDGYCK LFITKEEAVK RVRAWVGFDA EGAHATRDSI 

      6010       6020       6030       6040       6050       6060 
GTNFPLQLGF STGIDFVVEA TGLFADRDGY SFKKAVAKAP PGEQFKHLIP LMTRGQRWDV 

      6070       6080       6090       6100       6110       6120 
VRPRIVQMFA DHLIDLSDCV VLVTWAANFE LTCLRYFAKV GREISCNVST KRATAYNSRT 

      6130       6140       6150       6160       6170       6180 
GYYGCWRHSV TCDYLYNPLI VDIQQWGYIG SLSSNHDLYC SVHKGAHVAS SDAIMTRCLA 

      6190       6200       6210       6220       6230       6240 
VYDCFCNNIN WNVEYPIISN ELSINTSCRV LQRVMLKAAM LCNRYTLCYD IGNPKAIACV 

      6250       6260       6270       6280       6290       6300 
KDFDFKFYDA QPIVKSVKTL LYFFEAHKDS FKDGLCMFWN CNVDKYPPNA VVCRFDTRVL 

      6310       6320       6330       6340       6350       6360 
NNLNLPGCNG GSLYVNKHAF HTKPFSRAAF EHLKPMPFFY YSDTPCVYMD GMDAKQVDYV 

      6370       6380       6390       6400       6410       6420 
PLKSATCITR CNLGGAVCLK HAEEYREYLE SYNTATTAGF TFWVYKTFDF YNLWNTFTKL 

      6430       6440       6450       6460       6470       6480 
QSLENVVYNL VKTGHYTGQA GEMPCAIIND KVVAKIDKED VVIFINNTTY PTNVAVELFA 

      6490       6500       6510       6520       6530       6540 
KRSIRHHPEL KLFRNLNIDV CWKHVIWDYA RESIFCSNTY GVCMYTDLKL IDKLNVLFDG 

      6550       6560       6570       6580       6590       6600 
RDNGALEAFK RSNNGVYIST TKVKSLSMIR GPPRAELNGV VVDKVGDTDC VFYFAVRKEG 

      6610       6620       6630       6640       6650       6660 
QDVIFSQFDS LRVSSNQSPQ GNLGSNEPGN VGGNDALATS TIFTQSRVIS SFTCRTDMEK 

      6670       6680       6690       6700       6710       6720 
DFIALDQDVF IQKYGLEDYA FEHIVYGNFN QKIIGGLHLL IGLYRRQQTS NLVIQEFVSY 

      6730       6740       6750       6760       6770       6780 
DSSIHSYFIT DEKSGGSKSV CTVIDILLDD FVALVKSLNL NCVSKVVNVN VDFKDFQFML 

      6790       6800       6810       6820       6830       6840 
WCNDEKVMTF YPRLQAASDW KPGYSMPVLY KYLNSPMERV SLWNYGKPVT LPTGCMMNVA 

      6850       6860       6870       6880       6890       6900 
KYTQLCQYLN TTTLAVPVNT RVLHLGAGSE KGVAPGSAVL RQWLPAGTIL RQWLPAGTIL 

      6910       6920       6930       6940       6950       6960 
VHNDLYPFVS DSVATYFGDC ITLPFDCQWD LIISDMYDLL LDIGVHVVRC SYIHCHMIRD 

      6970       6980       6990       7000       7010       7020 
KLALGGSVAI KITEFSWNAE LYKLMGYFAF WTVFCTNANA SSSEGFLIGI NYLGKPKVEI 

      7030       7040       7050 
DGNVMHAIIC FGEIPQFGTG VLIACLIWLN SRLSWLVMP 

« Hide

Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6U1.

References

[1]"Bovine coronavirus (Quebec strain) full-length genomic sequence."
Yoo D., Pei Y., Parker M.D., Cox G.J.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF220295 Genomic RNA. Translation: AAL40396.1. Sequence problems.
AF220295 Genomic RNA. Translation: AAL40397.1. Sequence problems.

3D structure databases

ProteinModelPortalP0C6X0.
SMRP0C6X0. Positions 3964-4114, 4238-4362, 6422-6790.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR022570. Coronavirus_NSP1.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR002705. Pept_C30/C16_sub.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF11963. DUF3477. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_CVBQ
AccessionPrimary (citable) accession number: P0C6X0
Secondary accession number(s): Q8V6W6, Q8V6W7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries