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P0C6W6

- R1AB_BCRP3

UniProt

P0C6W6 - R1AB_BCRP3

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Protein
Replicase polyprotein 1ab
Gene
rep, 1a-1b
Organism
Bat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.
Nsp9 is a ssRNA-binding protein By similarity.
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1802Cleavage By similarity
Sitei818 – 8192Cleavage; by PL-PRO By similarity
Active sitei1649 – 16491For PL-PRO activity By similarity
Active sitei1810 – 18101For PL-PRO activity By similarity
Sitei3238 – 32392Cleavage; by PL-PRO By similarity
Active sitei3279 – 32791For 3CL-PRO activity By similarity
Active sitei3383 – 33831For 3CL-PRO activity By similarity
Sitei3544 – 35452Cleavage; by 3CL-PRO By similarity
Sitei3834 – 38352Cleavage; by 3CL-PRO By similarity
Sitei3917 – 39182Cleavage; by 3CL-PRO By similarity
Sitei4115 – 41162Cleavage; by 3CL-PRO By similarity
Sitei4228 – 42292Cleavage; by 3CL-PRO By similarity
Sitei4367 – 43682Cleavage; by 3CL-PRO By similarity
Sitei5299 – 53002Cleavage; by 3CL-PRO By similarity
Sitei5900 – 59012Cleavage; by 3CL-PRO By similarity
Sitei6427 – 64282Cleavage; by 3CL-PRO By similarity
Sitei6773 – 67742Cleavage; by 3CL-PRO By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1727 – 176438C4-type By similarity
Add
BLAST
Zinc fingeri4302 – 431817 By similarity
Add
BLAST
Zinc fingeri4345 – 435814 By similarity
Add
BLAST
Nucleotide bindingi5581 – 55888ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. endonuclease activity Source: UniProtKB-KW
  6. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  7. helicase activity Source: UniProtKB-KW
  8. methyltransferase activity Source: InterPro
  9. omega peptidase activity Source: InterPro
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  5. suppression by virus of host translation Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral RNA genome replication Source: InterPro
  9. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
Taxonomic identifieri349344 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRhinolophus ferrumequinum (Greater horseshoe bat) [TaxID: 59479]
Rhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
Rhinolophus pearsonii [TaxID: 188571]
Rhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399]
ProteomesiUP000006570: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2201 – 222121Helical; Reviewed prediction
Add
BLAST
Transmembranei2312 – 233423Helical; Reviewed prediction
Add
BLAST
Transmembranei2349 – 236921Helical; Reviewed prediction
Add
BLAST
Transmembranei2753 – 277321Helical; Reviewed prediction
Add
BLAST
Transmembranei3020 – 304021Helical; Reviewed prediction
Add
BLAST
Transmembranei3059 – 307921Helical; Reviewed prediction
Add
BLAST
Transmembranei3081 – 310121Helical; Reviewed prediction
Add
BLAST
Transmembranei3103 – 312321Helical; Reviewed prediction
Add
BLAST
Transmembranei3140 – 316021Helical; Reviewed prediction
Add
BLAST
Transmembranei3562 – 358221Helical; Reviewed prediction
Add
BLAST
Transmembranei3584 – 360421Helical; Reviewed prediction
Add
BLAST
Transmembranei3610 – 363021Helical; Reviewed prediction
Add
BLAST
Transmembranei3657 – 367620Helical; Reviewed prediction
Add
BLAST
Transmembranei3683 – 370220Helical; Reviewed prediction
Add
BLAST
Transmembranei3726 – 374621Helical; Reviewed prediction
Add
BLAST
Transmembranei3754 – 377421Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Non-structural protein 1 By similarity
PRO_0000043079Add
BLAST
Chaini180 – 818639Non-structural protein 2 By similarity
PRO_0000043080Add
BLAST
Chaini819 – 27381920Non-structural protein 3 By similarity
PRO_0000043081Add
BLAST
Chaini2739 – 3238500Non-structural protein 4 By similarity
PRO_0000283840Add
BLAST
Chaini3239 – 35443063C-like proteinase By similarity
PRO_0000043082Add
BLAST
Chaini3545 – 3834290Non-structural protein 6 By similarity
PRO_0000043083Add
BLAST
Chaini3835 – 391783Non-structural protein 7 By similarity
PRO_0000043084Add
BLAST
Chaini3918 – 4115198Non-structural protein 8 By similarity
PRO_0000043085Add
BLAST
Chaini4116 – 4228113Non-structural protein 9 By similarity
PRO_0000043086Add
BLAST
Chaini4229 – 4367139Non-structural protein 10 By similarity
PRO_0000043087Add
BLAST
Chaini4368 – 5299932RNA-directed RNA polymerase By similarity
PRO_0000043088Add
BLAST
Chaini5300 – 5900601Helicase By similarity
PRO_0000043089Add
BLAST
Chaini5901 – 6427527Exoribonuclease By similarity
PRO_0000043090Add
BLAST
Chaini6428 – 6773346Uridylate-specific endoribonuclease By similarity
PRO_0000043091Add
BLAST
Chaini6774 – 7071298Putative 2'-O-methyl transferase By similarity
PRO_0000043092Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6W6.
SMRiP0C6W6. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360, 6428-6771.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1001 – 1167167Macro
Add
BLAST
Domaini1609 – 1873265Peptidase C16
Add
BLAST
Domaini3239 – 3544306Peptidase C30
Add
BLAST
Domaini4979 – 5141163RdRp catalytic
Add
BLAST
Domaini5300 – 538384CV MBD
Add
BLAST
Domaini5556 – 5737182(+)RNA virus helicase ATP-binding
Add
BLAST
Domaini5738 – 5907170(+)RNA virus helicase C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2201 – 2369169HD1 By similarity
Add
BLAST
Regioni2753 – 3160408HD2 By similarity
Add
BLAST
Regioni3562 – 3774213HD3 By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi930 – 99970Glu-rich
Add
BLAST
Compositional biasi2208 – 22114Poly-Leu
Compositional biasi3764 – 37674Poly-Cys

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MESLVLGINE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKSGT     50
CGIVELEKGV LPQPEQPYVF IKRSDAQGTD HGHRVRELVA ELDGVQYGRS 100
GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG 150
TDPIEDYEQN WNTKHGSGVL RELTRELNGG ALTRYVDNNF CGPDGYPLEC 200
IKDLLARAGK SMCTLSEQLD YIESKRGVYC CRDHGHEIAW FTERSDKSYE 250
HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI 300
RSVYPVATPQ ECNNMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV 350
SEGPNTCGYL PTNAVVKMPC PACQDPEIGP EHSAADYHNH SNIETRLRKG 400
GRTRCFGGCV FAYVGCYNKR AYWVPRASAD IGSGHTGITG DNVETLNEDL 450
LEILSRERVN INIVGDFQLN EEVAIILASF SASTSAFIDT IKSLDYKSFK 500
TIVESCGNYK VTKGKPIKGA WNIGQHRSVL TPLCGFPSQA AGVIRSIFSR 550
TLDAANHSIP DLQRAAVTIL DSISEQSLRL VDAMVYTSNL LTNSVIIMAY 600
VTGGLVQQTS QWLSNLLDTT VEKLRPIFAW IEAKLSAGVE FLKDAWEILK 650
FLITGVFDIV KGQIQVASDN IKDCVKCFVD VVNKALEMCI DQVTIAGAKL 700
RSLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDSHDTV 750
LTSEEVVLKN GELEALETPV DSFTNGAVVG TPVCINGLML LEIKANEQYC 800
ALSPGLLATN NVFRLKGGAP TKGVTFGEDT VVEVQGYKNV RITFELDERV 850
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE 900
WSVATFYLFD DSGEEKLSSR MYCSFYPPDE EEDCEEYEEE EEVSERTCEH 950
EYGTEEDYKG LPLEFGASTD IIQVEEQEEE DWLDDAVEAE PEPEPLHEEP 1000
VNQLTGYLKL TDNVAIKCVD IVEEAQNANP MVIVNAANIH LKHGGGVAGA 1050
LNKATNGAMQ KESDHYIKLN GPLTVGGSCL LSGHNLAKKC LHVVGPNLNA 1100
GEDIQLLKAA YENFNSQDIL LAPLLSAGIF GAKPLQSLQM CVQTVRTQVY 1150
IVVNDKVLYE QVVMDYLDSL KPKVEAPKQE VLPKAEYPKV DEKSVVQKTI 1200
DVKPKIKACI DEVTTTLEET KFLTNKLLLF TDINGKLYQD SKNMLRGEDM 1250
SFLEKDAPYM VGDVITSGDI TCVVIPSKKA GGTTEMLSRA LKKVPINEYI 1300
TTYPGQGCAG YTLEEAKTAL KKCKSAFYVL PSETPNAKEE ILGTVSWNLR 1350
EMLAHAEETR KLMPVCMDVR AIMATIQRKY KGIKIQEGIV DYGVRFFFYT 1400
SKEPVASIIT KLNSLNEPLV TMPIGYVTHG FNLEEAARCM RSLKAPAIVS 1450
VSSPDAVTTY NGYLTSSSKT SEDHFVETVS LAGSYRDWSY SGQRTELGVE 1500
FLKRGEKIVY HTLESPVKFH LDGEVLPLDK LKSLLSLREV KTIKVFTTVD 1550
NTNLHTQLVD MSMTYGQQLG PTYLEGADVT KIKPHVNHEG KTFFVLPSDD 1600
TLRSEAFEYY HTLDESFLGR YMSALNHTKK WKFPQVGGLT SIKWADNNCY 1650
LSSVLLALQQ IEVKFNAPAL QEAYYRARAG DAANFCALIL AYSNKTVGEL 1700
GDVRETMTHL LQHANLESAK RVLNVVCKHC GQKTTTLTGV EAVMYMGTLS 1750
YDNLKMGVSI PCVCGRDATQ YLVQQESSFV MMSAPPAEYK LQQGTFLCAN 1800
EYTGNYQCGH YTHITAKETL YRIDGAHLTK MSEYKGPVTD VFYKETSYTT 1850
TIKPVSYKLD GVTYTEIEPK LDGYYKKDNA YYTEQPIDLI PTQPLPNASF 1900
DNFKLTCSNT KFADDLNQMT GFTKPASREL SVTFFPDLNG DVVAIDYRHY 1950
SASFKKGAKL LHKPIVWHIN QATTKTTFKP NTWCLRCLWS TKPVDTSNSF 2000
EVLAVEDTQG MDNLACESQQ PTPEEVVENP TIQKEVIECD VKTTEVVGNV 2050
ILKPSDEGVK VTQELDHEDL MAAYVENTSI TIKKPNELSL ALGLKTIATH 2100
GIAAINSVPW GKILAYVKPF LGQAAVTTSN CAKRLVQRMF NNYMPYVLTL 2150
LFQLCTFTKS TNSRIRASLP TTIAKNSVRG IVRLCLDAGI NYVKSPKFSK 2200
LFTIAMWLLL LSICLGSLIY VTAALGVLLS NFGAPSYCSG VRESYLNSSN 2250
VTTMDFCEGS FPCSVCLSGL DSLDSYPALE TIQVTISSYK LDLTILGLAA 2300
EWFFAYMLFT KFFYLLGLSA IMQVFFGYFA SHFISNSWLM WFIISIVQMA 2350
PVSAMVRMYI FFASFYYIWK SYVHIMDGCT SSTCMMCYKR NRATRVECTT 2400
IVNGMKRSFY VYANGGRGFC KTHNWNCLNC DTFCAGSTFI SDEVARDLSL 2450
QFKRPINPTD QSSYVVDSVA VKNGALHLYF DKAGQKTYER HPLSHFVNLD 2500
NLRANNTKGS LPINVIVFDG KSKCDESAAK SASVYYSQLM CQPILLLDQA 2550
LVSDVGDSTE VSVKMFDAYV DTFSATFSVP MEKLKALVAT AHSELAKGVA 2600
LDGVLSTFVS ASRQGVVDTD VDTKDVIECL KLSHHSDLEV TGDSCNNFML 2650
TYNKVENMTP RDLGACIDCN ARHINAQVAR SHNVSLIWNV KDYMSLSEQL 2700
RKQIRSAAKK NNIPFRLTCA TTRQVVNVIT TKISLKGGKI VSTWFKIMLK 2750
ATLLCVLAAL VCYIVMPVHI LSVHGGYTNE IIGYKAIQDG VTRDIVSTDD 2800
CFANKHAGFD SWFSQRGGSY KNDKSCPVVA AIITREIGFI VPGLPGTVLR 2850
AINGDFLHFL PRVFSAVGNI CYTPSKLIEY SDFSTSACVL AAECTIFKDA 2900
MGKPVPYCYD TNLLEGSISY SELRPDTRYV LMDGSIIQFP NAYLEGSVRV 2950
VTTFDAEYCR HGTCERSEAG ICLSTSGRWV LNNEHYRALP GVFCGVDAMN 3000
LIANIFTPLV QPVGALDVSA SVVAGGIIAI LVTCAAYYFM KFRRAFGEYN 3050
HVVAANAPLF LMSFTILCLA PAYSFLPGVY SVFYLYLTFY FTNDVSFLAH 3100
LQWFAMFSPI VPFWITAIYV FCISLKHFHW FFNNYLRKRV VFNGVTFSTF 3150
EEAALCTFLL NKEMYLKLRS ETLLPLTQYN RYLALYNKYK YFSGALDTTS 3200
YREAACCHLA KALNDFSNSG ADVLYQPPQT SITSAVLQSG FRKMAFPSGK 3250
VEGCMVQVTC GTTTLNGLWL DDTVYCPRHV ICTAEDMLNP NYEDLLIRKS 3300
NHSFLVQAGN VQLRVIGHSM QNCLLRLKVD TSNPKTPKYK FVRIQPGQTF 3350
SVLACYNGSP SGVYQCAMRP NHTIKGSFLN GSCGSVGFNI DYDCVSFCYM 3400
HHMELPTEVH AGTDLEGKFY GPFVDRQTAQ AAGTDTTITL NVLAWLYAAV 3450
INGDRWFLNR FTTTLNDFNL VAMKYNYEPL TQDHVDILGP LSAQTGIAVL 3500
DMCAALKELL QNGMNGRTIL GSTILEDEFT PFDVVRQCSG VTFQGKFKRI 3550
VKGTHHWMLL TFLTSLLILV QSTQWSLFFF VYENAFLPFT LGIMAVAACA 3600
MLLVKHKHAF LCLFLLPSLA TVAYFNMVYM PASWVMRIMT WLELADTSLS 3650
GYRLKDCVMY ASALVLLVLM TARTVYDDAA RRVWTLMNVI TLVYKVYYGN 3700
ALDQAISMWA LVISVTSNYS GVVTTIMFLA RAIVFVCVEY YPLLFITGNT 3750
LQCIMLVYCF LGYCCCCYFG LFCLLNRYFR LTLGVYDYLV STQEFRYMNS 3800
QGLLPPKSSI DAFKLNIKLL GIGGKPCIKV ATVQSKMSDV KCTSVVLLSV 3850
LQQLRVESSS KLWAQCVQLH NDILLAKDTT EAFEKMVSLL SVLLSMQGAV 3900
DINKLCEEML DNRATLQAIA SEFSSLPSYA AYATAQEAYE QAVANGDSEV 3950
VLKKLKKSLN VAKSEFDRNA AMQRKLEKMA DQAMTQMYKQ ARSEDKRAKV 4000
TSAMQTMLFT MLRKLDNDAL NNIINNARDG CVPLNIIPLT TAAKLMVVVP 4050
DYGTYKNTCD GNTFTYASAL WEIQQVVDAD SKIVQLSEIN MENSSNLAWP 4100
LIVTALRANS AVKLQNNELS PVALRQMSCA AGTTQTACTD DNALAYYNNS 4150
KGGRFVLALL SDHQDLKWAR FPKSDGTGTI YTELEPPCRF VTDTPKGPKV 4200
KYLHFIKGLN NLNRGMVLGS LAATVRLQAG NATEVPANST VLSFCAFAVD 4250
PAKAYKDYLA SGGQPITNCV KMLCTHTGTG QAITVTPEAN MDQESFGGAS 4300
CCLYCRCHID HPNPKGFCDL KGKYVQIPTT CANDPVGFTL RNTVCTVCGM 4350
WKGYGCSCDQ LREPMMQSAD ASTFLNRVCG VSAARLTPCG TGISTDVVYR 4400
AFDIYNEKVA GFAKFLKTNC CRFQEKDEEG NLLDSYFVVK RHTMSNYQHE 4450
ETIYNLVKDC PAVAVHDFFK FRVDGDMVPH ISRQRLTKYT MADLVYALRH 4500
FDEGNCDTLK EILVTYNCCD DDYFNKKDWY DFVENPDILR VYANLGERVR 4550
QALLKTVQFC DAMRDAGIVG VLTLDNQDLN GNWYDFGDFV QVAPGCGVPI 4600
VDSYYSLLMP ILTLTRALAA ESHMDADLAK PLIKWDLLKY DFTAERLCLF 4650
DRYFKYWDQT YHPNCINCLD DRCILHCANF NVLFSTVFPP TSFGPLVRKI 4700
FVDGVPFVVS TGYHFRELGV VHNQDVNLHS SRLSFKELLV YAADPAMHAA 4750
SGNLLLDKRT TCFSVAALTN NVAFQTVKPG NFNKDFYDFA VSKGFFKEGS 4800
SVELKHFFFA QDGNAAISDY DYYRYNLPTM CDIRQLLFVV EVVDKYFDCY 4850
DGGCINANQV IVNNLDKSAG FPFNKWGKAR LYYDSMSYED QDALFAYTKR 4900
NVIPTITQMN LKYAISAKNR ARTVAGVSIC STMTNRQFHQ KLLKSIAATR 4950
GATVVIGTSK FYGGWHNMLK TVYSDVETPH LMGWDYPKCD RAMPNMLRIM 5000
ASLVLARKHS TCCNLSHRFY RLANECAQVL SEMVMCGGSL YVKPGGTSSG 5050
DATTAYANSV FNICQAVTAN VNALLSTDGN KIADKYVRNL QHRLYECLYR 5100
NRDVDHEFVG EFYAYLRKHF SMMILSDDAV VCYNSNYAAQ GLVASIKNFK 5150
AVLYYQNNVF MSEAKCWTET DLTKGPHEFC SQHTMLVKQG DDYVYLPYPD 5200
PSRILGAGCF VDDIVKTDGT LMIERFVSLA IDAYPLTKHP NQEYADVFHL 5250
YLQYIRKLHD ELTGHMLDMY SVMLTNDNTS RYWEPEFYEA MYTPHTVLQA 5300
VGACVLCNSQ TSLRCGACIR RPFLCCKCCY DHVISTSHKL VLSVNPYVCN 5350
APGCDVTDVT QLYLGGMSYY CKSHKPPISF PLCANGQVFG LYKNTCVGSD 5400
NVTDFNAIAT CDWTNAGDYI LANTCTERLK LFAAETLKAT EETFKLSYGI 5450
ATVREVLSDR ELHLSWEVGK PRPPLNRNYV FTGYRVTKNS KVQIGEYTFE 5500
KGDYGDAVVY RGTTTYKLNI GDYFVLTSHT VMPLSAPTLV PQEHYVRITG 5550
LYPTLNISDE FSSNVANYQK VGMQKYSTLQ GPPGTGKSHF AIGLALYYPS 5600
ARIVYTACSH AAVDALCEKA LKYLPIDKCS RIIPARARVE CFDKFKVNST 5650
LEQYVFCTVN ALPETTADIV VFDEISMATN YDLSVVNARL RAKHYVYIGD 5700
PAQLPAPRTL LTKGTLEPEY FNSVCRLMKT IGPDMFLGTC RRCPAEIVDT 5750
VSALVYDNKL KAHKEKSAQC FKMFYKGVIT HDVSSAINRP QIGVVREFLT 5800
RNPAWRKAVF ISPYNSQNAV ASKILGLPTQ TVDSSQGSEY DYVIFTQTTE 5850
TAHSCNVNRF NVAITRAKIG ILCIMSDRDL YDKLQFTSLE VPRRNVATLQ 5900
AENVTGLFKD CSKIITGLHP TQAPTHLSVD TKFKTEGLCV DIPGIPKDMT 5950
YRRLISMMGF KMNYQVNGYP NMFITREEAI RHVRAWIGFD VEGCHATRDA 6000
VGTNLPLQLG FSTGVNLVAI PTGYVDTENN TEFTRVNAKP PPGDQFKHLI 6050
PLMYKGLPWN VVRIKIVQML SDTLKGLSDR VVFVLWAHGF ELTSMKYFVK 6100
IGPERTCCLC DKRATCFSTS SDTYACWNHS VGFDYVYNPF MIDVQQWGFT 6150
GNLQSNHDQH CQVHGNAHVA SCDAIMTRCL AVHECFVKRV DWSVEYPIIG 6200
DELKINSACR KVQHMVVKSA LLADKFPVLH DIGNPKAIKC VPQAEVEWKF 6250
YDAQPCSDKA YKIEELFYSY ATHHDKFTDG VCLFWNCNVD RYPANAIVCR 6300
FDTRVLSNLN LPGCDGGSLY VNKHAFHTPA FDKSAFTNLK QLPFFYYSDS 6350
PCESHGKQVV SDIDYVPLKS ATCITRCNLG GAVCRHHANE YRQYLDAYNM 6400
MISAGFSLWI YKQFDTYNLW NTFTRLQSLE NVAYNVVNKG HFDGQAGETP 6450
VSIINNAVYT KVDGFDVEIF ENKTTLPVNV AFELWAKRNI KSVPEIKILN 6500
NLGVDIAANT VIWDHKREAP VHMSTIGVCT MTDIAKKPTE SACSSLTVLF 6550
DGRVEGQVDL FRNARNGVLI TEGSVKGLTP SKGPAQASVN GVTLIGESVK 6600
TQFNYFKKVD GIIQQLPETY FTQSRDLEDF KPRSQMETDF LELAMDEFIQ 6650
RYKLEGYAFE HIVYGDFSHG QLGGLHLMIG LAKRSRDSPL KLEDFIPMDS 6700
TVKNYFITDA QTGSSKCVCS VIDLLLDDFV EIIKSQDLSV VSKVVKVTID 6750
YAEISFMLWC KDGHVETFYP KLQASQAWQP GVAMPNLYKM QRMLLEKCDL 6800
QNYGENAVIP KGIMMNVAKY TQLCQYLNTL TLAVPYNMRV IHFGAGSDKG 6850
VAPGTAVLRQ WLPTGTLLVD SDLNDFVSDA DSTLIGDCAT VHTANKWDLI 6900
VSDMYDPKAK HVTKENDSKE GFFTYLCGFI KQKLALGGSV AVKITEHSWN 6950
ADLYKLMGHF SWWTAFVTNV NASSSEAFLI GVNYLGKPKE QIDGYTMHAN 7000
YIFWRNTNPI QLSSYSLFDM SKFPLKLRGT AVMSLKENQI NDMIYSLLEK 7050
GRLIIRENNR VVVSSDILVN N 7071

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,071
Mass (Da):790,103
Last modified:June 10, 2008 - v1
Checksum:i711112FAF32354AB
GO
Isoform Replicase polyprotein 1a (identifier: P0C6T7-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6T7.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,380
Mass (Da):486,337
GO

Sequence cautioni

The sequence AAZ67050.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAZ67051.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ071615 Genomic RNA. Translation: AAZ67050.1. Sequence problems.
DQ071615 Genomic RNA. Translation: AAZ67051.1. Sequence problems.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ071615 Genomic RNA. Translation: AAZ67050.1 . Sequence problems.
DQ071615 Genomic RNA. Translation: AAZ67051.1 . Sequence problems.

3D structure databases

ProteinModelPortali P0C6W6.
SMRi P0C6W6. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360, 6428-6771.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1AB_BCRP3
AccessioniPrimary (citable) accession number: P0C6W6
Secondary accession number(s): Q3I5J6, Q3I5J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi