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P0C6W6

- R1AB_BCRP3

UniProt

P0C6W6 - R1AB_BCRP3

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Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity
Host translation inhibitor nsp1: Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.By similarity
Non-structural protein 2: May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.By similarity
Papain-like proteinase: Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.By similarity
Non-structural protein 4: Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.By similarity
Proteinase 3CL-PRO: Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).By similarityPROSITE-ProRule annotation
Non-structural protein 6: Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.By similarity
Non-structural protein 7: Forms an hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity
Non-structural protein 8: Forms an hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity
Non-structural protein 9: May participate in viral replication by acting as a ssRNA-binding protein.By similarity
Non-structural protein 10: Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.By similarity
RNA-directed RNA polymerase: Responsible for replication and transcription of the viral RNA genome.By similarity
Helicase: Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.By similarity
Guanine-N7 methyltransferase: Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.By similarity
Uridylate-specific endoribonuclease: Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
2'-O-methyltransferase: Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1802CleavageBy similarity
Sitei818 – 8192Cleavage; by PL-PROBy similarity
Active sitei1649 – 16491For PL-PRO activityPROSITE-ProRule annotation
Active sitei1810 – 18101For PL-PRO activityPROSITE-ProRule annotation
Sitei3238 – 32392Cleavage; by PL-PROBy similarity
Active sitei3279 – 32791For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3383 – 33831For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3544 – 35452Cleavage; by 3CL-PROBy similarity
Sitei3834 – 38352Cleavage; by 3CL-PROBy similarity
Sitei3917 – 39182Cleavage; by 3CL-PROBy similarity
Sitei4115 – 41162Cleavage; by 3CL-PROBy similarity
Sitei4228 – 42292Cleavage; by 3CL-PROBy similarity
Sitei4367 – 43682Cleavage; by 3CL-PROBy similarity
Sitei5299 – 53002Cleavage; by 3CL-PROBy similarity
Sitei5900 – 59012Cleavage; by 3CL-PROBy similarity
Sitei6427 – 64282Cleavage; by 3CL-PROBy similarity
Sitei6773 – 67742Cleavage; by 3CL-PROBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1727 – 176438C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4302 – 431817By similarityAdd
BLAST
Zinc fingeri4345 – 435814By similarityAdd
BLAST
Nucleotide bindingi5581 – 55888ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. methyltransferase activity Source: InterPro
  7. omega peptidase activity Source: InterPro
  8. RNA binding Source: UniProtKB-KW
  9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host gene expression Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral protein processing Source: InterPro
  9. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Inhibition of host NF-kappa-B by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Papain-like proteinase (EC:3.4.19.12, EC:3.4.22.69)
Short name:
PL-PRO
Alternative name(s):
Non-structural protein 3
Short name:
nsp3
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
Taxonomic identifieri349344 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRhinolophus ferrumequinum (Greater horseshoe bat) [TaxID: 59479]
Rhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
Rhinolophus pearsonii [TaxID: 188571]
Rhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399]
ProteomesiUP000006570: Genome

Subcellular locationi

Chain Papain-like proteinase : Host membrane; Multi-pass membrane protein. Host cytoplasm By similarity
Chain Non-structural protein 4 : Host membrane; Multi-pass membrane protein. Host cytoplasm
Note: Localizes in virally-induced cytoplasmic double-membrane vesicles.By similarity
Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2201 – 222121HelicalSequence AnalysisAdd
BLAST
Transmembranei2312 – 233423HelicalSequence AnalysisAdd
BLAST
Transmembranei2349 – 236921HelicalSequence AnalysisAdd
BLAST
Transmembranei2753 – 277321HelicalSequence AnalysisAdd
BLAST
Transmembranei3020 – 304021HelicalSequence AnalysisAdd
BLAST
Transmembranei3059 – 307921HelicalSequence AnalysisAdd
BLAST
Transmembranei3081 – 310121HelicalSequence AnalysisAdd
BLAST
Transmembranei3103 – 312321HelicalSequence AnalysisAdd
BLAST
Transmembranei3140 – 316021HelicalSequence AnalysisAdd
BLAST
Transmembranei3562 – 358221HelicalSequence AnalysisAdd
BLAST
Transmembranei3584 – 360421HelicalSequence AnalysisAdd
BLAST
Transmembranei3610 – 363021HelicalSequence AnalysisAdd
BLAST
Transmembranei3657 – 367620HelicalSequence AnalysisAdd
BLAST
Transmembranei3683 – 370220HelicalSequence AnalysisAdd
BLAST
Transmembranei3726 – 374621HelicalSequence AnalysisAdd
BLAST
Transmembranei3754 – 377421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Host translation inhibitor nsp1By similarityPRO_0000043079Add
BLAST
Chaini180 – 818639Non-structural protein 2By similarityPRO_0000043080Add
BLAST
Chaini819 – 27381920Papain-like proteinaseBy similarityPRO_0000043081Add
BLAST
Chaini2739 – 3238500Non-structural protein 4By similarityPRO_0000283840Add
BLAST
Chaini3239 – 35443063C-like proteinaseBy similarityPRO_0000043082Add
BLAST
Chaini3545 – 3834290Non-structural protein 6By similarityPRO_0000043083Add
BLAST
Chaini3835 – 391783Non-structural protein 7By similarityPRO_0000043084Add
BLAST
Chaini3918 – 4115198Non-structural protein 8By similarityPRO_0000043085Add
BLAST
Chaini4116 – 4228113Non-structural protein 9By similarityPRO_0000043086Add
BLAST
Chaini4229 – 4367139Non-structural protein 10By similarityPRO_0000043087Add
BLAST
Chaini4368 – 5299932RNA-directed RNA polymeraseBy similarityPRO_0000043088Add
BLAST
Chaini5300 – 5900601HelicaseBy similarityPRO_0000043089Add
BLAST
Chaini5901 – 6427527Guanine-N7 methyltransferaseBy similarityPRO_0000043090Add
BLAST
Chaini6428 – 6773346Uridylate-specific endoribonucleaseBy similarityPRO_0000043091Add
BLAST
Chaini6774 – 70712982'-O-methyltransferaseBy similarityPRO_0000043092Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Interactioni

Subunit structurei

Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6W6.
SMRiP0C6W6. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360, 6428-6771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1001 – 1167167MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1609 – 1873265Peptidase C16PROSITE-ProRule annotationAdd
BLAST
Domaini3239 – 3544306Peptidase C30PROSITE-ProRule annotationAdd
BLAST
Domaini4979 – 5141163RdRp catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini5300 – 538384CV MBDAdd
BLAST
Domaini5556 – 5737182(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini5738 – 5907170(+)RNA virus helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2201 – 2369169HD1By similarityAdd
BLAST
Regioni2753 – 3160408HD2By similarityAdd
BLAST
Regioni3562 – 3774213HD3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi930 – 99970Glu-richAdd
BLAST
Compositional biasi2208 – 22114Poly-Leu
Compositional biasi3764 – 37674Poly-Cys

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1727 – 176438C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4302 – 431817By similarityAdd
BLAST
Zinc fingeri4345 – 435814By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MESLVLGINE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKSGT
60 70 80 90 100
CGIVELEKGV LPQPEQPYVF IKRSDAQGTD HGHRVRELVA ELDGVQYGRS
110 120 130 140 150
GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG
160 170 180 190 200
TDPIEDYEQN WNTKHGSGVL RELTRELNGG ALTRYVDNNF CGPDGYPLEC
210 220 230 240 250
IKDLLARAGK SMCTLSEQLD YIESKRGVYC CRDHGHEIAW FTERSDKSYE
260 270 280 290 300
HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI
310 320 330 340 350
RSVYPVATPQ ECNNMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV
360 370 380 390 400
SEGPNTCGYL PTNAVVKMPC PACQDPEIGP EHSAADYHNH SNIETRLRKG
410 420 430 440 450
GRTRCFGGCV FAYVGCYNKR AYWVPRASAD IGSGHTGITG DNVETLNEDL
460 470 480 490 500
LEILSRERVN INIVGDFQLN EEVAIILASF SASTSAFIDT IKSLDYKSFK
510 520 530 540 550
TIVESCGNYK VTKGKPIKGA WNIGQHRSVL TPLCGFPSQA AGVIRSIFSR
560 570 580 590 600
TLDAANHSIP DLQRAAVTIL DSISEQSLRL VDAMVYTSNL LTNSVIIMAY
610 620 630 640 650
VTGGLVQQTS QWLSNLLDTT VEKLRPIFAW IEAKLSAGVE FLKDAWEILK
660 670 680 690 700
FLITGVFDIV KGQIQVASDN IKDCVKCFVD VVNKALEMCI DQVTIAGAKL
710 720 730 740 750
RSLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDSHDTV
760 770 780 790 800
LTSEEVVLKN GELEALETPV DSFTNGAVVG TPVCINGLML LEIKANEQYC
810 820 830 840 850
ALSPGLLATN NVFRLKGGAP TKGVTFGEDT VVEVQGYKNV RITFELDERV
860 870 880 890 900
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE
910 920 930 940 950
WSVATFYLFD DSGEEKLSSR MYCSFYPPDE EEDCEEYEEE EEVSERTCEH
960 970 980 990 1000
EYGTEEDYKG LPLEFGASTD IIQVEEQEEE DWLDDAVEAE PEPEPLHEEP
1010 1020 1030 1040 1050
VNQLTGYLKL TDNVAIKCVD IVEEAQNANP MVIVNAANIH LKHGGGVAGA
1060 1070 1080 1090 1100
LNKATNGAMQ KESDHYIKLN GPLTVGGSCL LSGHNLAKKC LHVVGPNLNA
1110 1120 1130 1140 1150
GEDIQLLKAA YENFNSQDIL LAPLLSAGIF GAKPLQSLQM CVQTVRTQVY
1160 1170 1180 1190 1200
IVVNDKVLYE QVVMDYLDSL KPKVEAPKQE VLPKAEYPKV DEKSVVQKTI
1210 1220 1230 1240 1250
DVKPKIKACI DEVTTTLEET KFLTNKLLLF TDINGKLYQD SKNMLRGEDM
1260 1270 1280 1290 1300
SFLEKDAPYM VGDVITSGDI TCVVIPSKKA GGTTEMLSRA LKKVPINEYI
1310 1320 1330 1340 1350
TTYPGQGCAG YTLEEAKTAL KKCKSAFYVL PSETPNAKEE ILGTVSWNLR
1360 1370 1380 1390 1400
EMLAHAEETR KLMPVCMDVR AIMATIQRKY KGIKIQEGIV DYGVRFFFYT
1410 1420 1430 1440 1450
SKEPVASIIT KLNSLNEPLV TMPIGYVTHG FNLEEAARCM RSLKAPAIVS
1460 1470 1480 1490 1500
VSSPDAVTTY NGYLTSSSKT SEDHFVETVS LAGSYRDWSY SGQRTELGVE
1510 1520 1530 1540 1550
FLKRGEKIVY HTLESPVKFH LDGEVLPLDK LKSLLSLREV KTIKVFTTVD
1560 1570 1580 1590 1600
NTNLHTQLVD MSMTYGQQLG PTYLEGADVT KIKPHVNHEG KTFFVLPSDD
1610 1620 1630 1640 1650
TLRSEAFEYY HTLDESFLGR YMSALNHTKK WKFPQVGGLT SIKWADNNCY
1660 1670 1680 1690 1700
LSSVLLALQQ IEVKFNAPAL QEAYYRARAG DAANFCALIL AYSNKTVGEL
1710 1720 1730 1740 1750
GDVRETMTHL LQHANLESAK RVLNVVCKHC GQKTTTLTGV EAVMYMGTLS
1760 1770 1780 1790 1800
YDNLKMGVSI PCVCGRDATQ YLVQQESSFV MMSAPPAEYK LQQGTFLCAN
1810 1820 1830 1840 1850
EYTGNYQCGH YTHITAKETL YRIDGAHLTK MSEYKGPVTD VFYKETSYTT
1860 1870 1880 1890 1900
TIKPVSYKLD GVTYTEIEPK LDGYYKKDNA YYTEQPIDLI PTQPLPNASF
1910 1920 1930 1940 1950
DNFKLTCSNT KFADDLNQMT GFTKPASREL SVTFFPDLNG DVVAIDYRHY
1960 1970 1980 1990 2000
SASFKKGAKL LHKPIVWHIN QATTKTTFKP NTWCLRCLWS TKPVDTSNSF
2010 2020 2030 2040 2050
EVLAVEDTQG MDNLACESQQ PTPEEVVENP TIQKEVIECD VKTTEVVGNV
2060 2070 2080 2090 2100
ILKPSDEGVK VTQELDHEDL MAAYVENTSI TIKKPNELSL ALGLKTIATH
2110 2120 2130 2140 2150
GIAAINSVPW GKILAYVKPF LGQAAVTTSN CAKRLVQRMF NNYMPYVLTL
2160 2170 2180 2190 2200
LFQLCTFTKS TNSRIRASLP TTIAKNSVRG IVRLCLDAGI NYVKSPKFSK
2210 2220 2230 2240 2250
LFTIAMWLLL LSICLGSLIY VTAALGVLLS NFGAPSYCSG VRESYLNSSN
2260 2270 2280 2290 2300
VTTMDFCEGS FPCSVCLSGL DSLDSYPALE TIQVTISSYK LDLTILGLAA
2310 2320 2330 2340 2350
EWFFAYMLFT KFFYLLGLSA IMQVFFGYFA SHFISNSWLM WFIISIVQMA
2360 2370 2380 2390 2400
PVSAMVRMYI FFASFYYIWK SYVHIMDGCT SSTCMMCYKR NRATRVECTT
2410 2420 2430 2440 2450
IVNGMKRSFY VYANGGRGFC KTHNWNCLNC DTFCAGSTFI SDEVARDLSL
2460 2470 2480 2490 2500
QFKRPINPTD QSSYVVDSVA VKNGALHLYF DKAGQKTYER HPLSHFVNLD
2510 2520 2530 2540 2550
NLRANNTKGS LPINVIVFDG KSKCDESAAK SASVYYSQLM CQPILLLDQA
2560 2570 2580 2590 2600
LVSDVGDSTE VSVKMFDAYV DTFSATFSVP MEKLKALVAT AHSELAKGVA
2610 2620 2630 2640 2650
LDGVLSTFVS ASRQGVVDTD VDTKDVIECL KLSHHSDLEV TGDSCNNFML
2660 2670 2680 2690 2700
TYNKVENMTP RDLGACIDCN ARHINAQVAR SHNVSLIWNV KDYMSLSEQL
2710 2720 2730 2740 2750
RKQIRSAAKK NNIPFRLTCA TTRQVVNVIT TKISLKGGKI VSTWFKIMLK
2760 2770 2780 2790 2800
ATLLCVLAAL VCYIVMPVHI LSVHGGYTNE IIGYKAIQDG VTRDIVSTDD
2810 2820 2830 2840 2850
CFANKHAGFD SWFSQRGGSY KNDKSCPVVA AIITREIGFI VPGLPGTVLR
2860 2870 2880 2890 2900
AINGDFLHFL PRVFSAVGNI CYTPSKLIEY SDFSTSACVL AAECTIFKDA
2910 2920 2930 2940 2950
MGKPVPYCYD TNLLEGSISY SELRPDTRYV LMDGSIIQFP NAYLEGSVRV
2960 2970 2980 2990 3000
VTTFDAEYCR HGTCERSEAG ICLSTSGRWV LNNEHYRALP GVFCGVDAMN
3010 3020 3030 3040 3050
LIANIFTPLV QPVGALDVSA SVVAGGIIAI LVTCAAYYFM KFRRAFGEYN
3060 3070 3080 3090 3100
HVVAANAPLF LMSFTILCLA PAYSFLPGVY SVFYLYLTFY FTNDVSFLAH
3110 3120 3130 3140 3150
LQWFAMFSPI VPFWITAIYV FCISLKHFHW FFNNYLRKRV VFNGVTFSTF
3160 3170 3180 3190 3200
EEAALCTFLL NKEMYLKLRS ETLLPLTQYN RYLALYNKYK YFSGALDTTS
3210 3220 3230 3240 3250
YREAACCHLA KALNDFSNSG ADVLYQPPQT SITSAVLQSG FRKMAFPSGK
3260 3270 3280 3290 3300
VEGCMVQVTC GTTTLNGLWL DDTVYCPRHV ICTAEDMLNP NYEDLLIRKS
3310 3320 3330 3340 3350
NHSFLVQAGN VQLRVIGHSM QNCLLRLKVD TSNPKTPKYK FVRIQPGQTF
3360 3370 3380 3390 3400
SVLACYNGSP SGVYQCAMRP NHTIKGSFLN GSCGSVGFNI DYDCVSFCYM
3410 3420 3430 3440 3450
HHMELPTEVH AGTDLEGKFY GPFVDRQTAQ AAGTDTTITL NVLAWLYAAV
3460 3470 3480 3490 3500
INGDRWFLNR FTTTLNDFNL VAMKYNYEPL TQDHVDILGP LSAQTGIAVL
3510 3520 3530 3540 3550
DMCAALKELL QNGMNGRTIL GSTILEDEFT PFDVVRQCSG VTFQGKFKRI
3560 3570 3580 3590 3600
VKGTHHWMLL TFLTSLLILV QSTQWSLFFF VYENAFLPFT LGIMAVAACA
3610 3620 3630 3640 3650
MLLVKHKHAF LCLFLLPSLA TVAYFNMVYM PASWVMRIMT WLELADTSLS
3660 3670 3680 3690 3700
GYRLKDCVMY ASALVLLVLM TARTVYDDAA RRVWTLMNVI TLVYKVYYGN
3710 3720 3730 3740 3750
ALDQAISMWA LVISVTSNYS GVVTTIMFLA RAIVFVCVEY YPLLFITGNT
3760 3770 3780 3790 3800
LQCIMLVYCF LGYCCCCYFG LFCLLNRYFR LTLGVYDYLV STQEFRYMNS
3810 3820 3830 3840 3850
QGLLPPKSSI DAFKLNIKLL GIGGKPCIKV ATVQSKMSDV KCTSVVLLSV
3860 3870 3880 3890 3900
LQQLRVESSS KLWAQCVQLH NDILLAKDTT EAFEKMVSLL SVLLSMQGAV
3910 3920 3930 3940 3950
DINKLCEEML DNRATLQAIA SEFSSLPSYA AYATAQEAYE QAVANGDSEV
3960 3970 3980 3990 4000
VLKKLKKSLN VAKSEFDRNA AMQRKLEKMA DQAMTQMYKQ ARSEDKRAKV
4010 4020 4030 4040 4050
TSAMQTMLFT MLRKLDNDAL NNIINNARDG CVPLNIIPLT TAAKLMVVVP
4060 4070 4080 4090 4100
DYGTYKNTCD GNTFTYASAL WEIQQVVDAD SKIVQLSEIN MENSSNLAWP
4110 4120 4130 4140 4150
LIVTALRANS AVKLQNNELS PVALRQMSCA AGTTQTACTD DNALAYYNNS
4160 4170 4180 4190 4200
KGGRFVLALL SDHQDLKWAR FPKSDGTGTI YTELEPPCRF VTDTPKGPKV
4210 4220 4230 4240 4250
KYLHFIKGLN NLNRGMVLGS LAATVRLQAG NATEVPANST VLSFCAFAVD
4260 4270 4280 4290 4300
PAKAYKDYLA SGGQPITNCV KMLCTHTGTG QAITVTPEAN MDQESFGGAS
4310 4320 4330 4340 4350
CCLYCRCHID HPNPKGFCDL KGKYVQIPTT CANDPVGFTL RNTVCTVCGM
4360 4370 4380 4390 4400
WKGYGCSCDQ LREPMMQSAD ASTFLNRVCG VSAARLTPCG TGISTDVVYR
4410 4420 4430 4440 4450
AFDIYNEKVA GFAKFLKTNC CRFQEKDEEG NLLDSYFVVK RHTMSNYQHE
4460 4470 4480 4490 4500
ETIYNLVKDC PAVAVHDFFK FRVDGDMVPH ISRQRLTKYT MADLVYALRH
4510 4520 4530 4540 4550
FDEGNCDTLK EILVTYNCCD DDYFNKKDWY DFVENPDILR VYANLGERVR
4560 4570 4580 4590 4600
QALLKTVQFC DAMRDAGIVG VLTLDNQDLN GNWYDFGDFV QVAPGCGVPI
4610 4620 4630 4640 4650
VDSYYSLLMP ILTLTRALAA ESHMDADLAK PLIKWDLLKY DFTAERLCLF
4660 4670 4680 4690 4700
DRYFKYWDQT YHPNCINCLD DRCILHCANF NVLFSTVFPP TSFGPLVRKI
4710 4720 4730 4740 4750
FVDGVPFVVS TGYHFRELGV VHNQDVNLHS SRLSFKELLV YAADPAMHAA
4760 4770 4780 4790 4800
SGNLLLDKRT TCFSVAALTN NVAFQTVKPG NFNKDFYDFA VSKGFFKEGS
4810 4820 4830 4840 4850
SVELKHFFFA QDGNAAISDY DYYRYNLPTM CDIRQLLFVV EVVDKYFDCY
4860 4870 4880 4890 4900
DGGCINANQV IVNNLDKSAG FPFNKWGKAR LYYDSMSYED QDALFAYTKR
4910 4920 4930 4940 4950
NVIPTITQMN LKYAISAKNR ARTVAGVSIC STMTNRQFHQ KLLKSIAATR
4960 4970 4980 4990 5000
GATVVIGTSK FYGGWHNMLK TVYSDVETPH LMGWDYPKCD RAMPNMLRIM
5010 5020 5030 5040 5050
ASLVLARKHS TCCNLSHRFY RLANECAQVL SEMVMCGGSL YVKPGGTSSG
5060 5070 5080 5090 5100
DATTAYANSV FNICQAVTAN VNALLSTDGN KIADKYVRNL QHRLYECLYR
5110 5120 5130 5140 5150
NRDVDHEFVG EFYAYLRKHF SMMILSDDAV VCYNSNYAAQ GLVASIKNFK
5160 5170 5180 5190 5200
AVLYYQNNVF MSEAKCWTET DLTKGPHEFC SQHTMLVKQG DDYVYLPYPD
5210 5220 5230 5240 5250
PSRILGAGCF VDDIVKTDGT LMIERFVSLA IDAYPLTKHP NQEYADVFHL
5260 5270 5280 5290 5300
YLQYIRKLHD ELTGHMLDMY SVMLTNDNTS RYWEPEFYEA MYTPHTVLQA
5310 5320 5330 5340 5350
VGACVLCNSQ TSLRCGACIR RPFLCCKCCY DHVISTSHKL VLSVNPYVCN
5360 5370 5380 5390 5400
APGCDVTDVT QLYLGGMSYY CKSHKPPISF PLCANGQVFG LYKNTCVGSD
5410 5420 5430 5440 5450
NVTDFNAIAT CDWTNAGDYI LANTCTERLK LFAAETLKAT EETFKLSYGI
5460 5470 5480 5490 5500
ATVREVLSDR ELHLSWEVGK PRPPLNRNYV FTGYRVTKNS KVQIGEYTFE
5510 5520 5530 5540 5550
KGDYGDAVVY RGTTTYKLNI GDYFVLTSHT VMPLSAPTLV PQEHYVRITG
5560 5570 5580 5590 5600
LYPTLNISDE FSSNVANYQK VGMQKYSTLQ GPPGTGKSHF AIGLALYYPS
5610 5620 5630 5640 5650
ARIVYTACSH AAVDALCEKA LKYLPIDKCS RIIPARARVE CFDKFKVNST
5660 5670 5680 5690 5700
LEQYVFCTVN ALPETTADIV VFDEISMATN YDLSVVNARL RAKHYVYIGD
5710 5720 5730 5740 5750
PAQLPAPRTL LTKGTLEPEY FNSVCRLMKT IGPDMFLGTC RRCPAEIVDT
5760 5770 5780 5790 5800
VSALVYDNKL KAHKEKSAQC FKMFYKGVIT HDVSSAINRP QIGVVREFLT
5810 5820 5830 5840 5850
RNPAWRKAVF ISPYNSQNAV ASKILGLPTQ TVDSSQGSEY DYVIFTQTTE
5860 5870 5880 5890 5900
TAHSCNVNRF NVAITRAKIG ILCIMSDRDL YDKLQFTSLE VPRRNVATLQ
5910 5920 5930 5940 5950
AENVTGLFKD CSKIITGLHP TQAPTHLSVD TKFKTEGLCV DIPGIPKDMT
5960 5970 5980 5990 6000
YRRLISMMGF KMNYQVNGYP NMFITREEAI RHVRAWIGFD VEGCHATRDA
6010 6020 6030 6040 6050
VGTNLPLQLG FSTGVNLVAI PTGYVDTENN TEFTRVNAKP PPGDQFKHLI
6060 6070 6080 6090 6100
PLMYKGLPWN VVRIKIVQML SDTLKGLSDR VVFVLWAHGF ELTSMKYFVK
6110 6120 6130 6140 6150
IGPERTCCLC DKRATCFSTS SDTYACWNHS VGFDYVYNPF MIDVQQWGFT
6160 6170 6180 6190 6200
GNLQSNHDQH CQVHGNAHVA SCDAIMTRCL AVHECFVKRV DWSVEYPIIG
6210 6220 6230 6240 6250
DELKINSACR KVQHMVVKSA LLADKFPVLH DIGNPKAIKC VPQAEVEWKF
6260 6270 6280 6290 6300
YDAQPCSDKA YKIEELFYSY ATHHDKFTDG VCLFWNCNVD RYPANAIVCR
6310 6320 6330 6340 6350
FDTRVLSNLN LPGCDGGSLY VNKHAFHTPA FDKSAFTNLK QLPFFYYSDS
6360 6370 6380 6390 6400
PCESHGKQVV SDIDYVPLKS ATCITRCNLG GAVCRHHANE YRQYLDAYNM
6410 6420 6430 6440 6450
MISAGFSLWI YKQFDTYNLW NTFTRLQSLE NVAYNVVNKG HFDGQAGETP
6460 6470 6480 6490 6500
VSIINNAVYT KVDGFDVEIF ENKTTLPVNV AFELWAKRNI KSVPEIKILN
6510 6520 6530 6540 6550
NLGVDIAANT VIWDHKREAP VHMSTIGVCT MTDIAKKPTE SACSSLTVLF
6560 6570 6580 6590 6600
DGRVEGQVDL FRNARNGVLI TEGSVKGLTP SKGPAQASVN GVTLIGESVK
6610 6620 6630 6640 6650
TQFNYFKKVD GIIQQLPETY FTQSRDLEDF KPRSQMETDF LELAMDEFIQ
6660 6670 6680 6690 6700
RYKLEGYAFE HIVYGDFSHG QLGGLHLMIG LAKRSRDSPL KLEDFIPMDS
6710 6720 6730 6740 6750
TVKNYFITDA QTGSSKCVCS VIDLLLDDFV EIIKSQDLSV VSKVVKVTID
6760 6770 6780 6790 6800
YAEISFMLWC KDGHVETFYP KLQASQAWQP GVAMPNLYKM QRMLLEKCDL
6810 6820 6830 6840 6850
QNYGENAVIP KGIMMNVAKY TQLCQYLNTL TLAVPYNMRV IHFGAGSDKG
6860 6870 6880 6890 6900
VAPGTAVLRQ WLPTGTLLVD SDLNDFVSDA DSTLIGDCAT VHTANKWDLI
6910 6920 6930 6940 6950
VSDMYDPKAK HVTKENDSKE GFFTYLCGFI KQKLALGGSV AVKITEHSWN
6960 6970 6980 6990 7000
ADLYKLMGHF SWWTAFVTNV NASSSEAFLI GVNYLGKPKE QIDGYTMHAN
7010 7020 7030 7040 7050
YIFWRNTNPI QLSSYSLFDM SKFPLKLRGT AVMSLKENQI NDMIYSLLEK
7060 7070
GRLIIRENNR VVVSSDILVN N

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,071
Mass (Da):790,103
Last modified:June 10, 2008 - v1
Checksum:i711112FAF32354AB
GO
Isoform Replicase polyprotein 1a (identifier: P0C6T7-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6T7.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,380
Mass (Da):486,337
GO

Sequence cautioni

The sequence AAZ67050.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAZ67051.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ071615 Genomic RNA. Translation: AAZ67050.1. Sequence problems.
DQ071615 Genomic RNA. Translation: AAZ67051.1. Sequence problems.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ071615 Genomic RNA. Translation: AAZ67050.1 . Sequence problems.
DQ071615 Genomic RNA. Translation: AAZ67051.1 . Sequence problems.

3D structure databases

ProteinModelPortali P0C6W6.
SMRi P0C6W6. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360, 6428-6771.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1AB_BCRP3
AccessioniPrimary (citable) accession number: P0C6W6
Secondary accession number(s): Q3I5J6, Q3I5J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3