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P0C6W6

- R1AB_BCRP3

UniProt

P0C6W6 - R1AB_BCRP3

Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity
    The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.By similarity
    The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
    Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    ATP + H2O = ADP + phosphate.
    TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei179 – 1802CleavageBy similarity
    Sitei818 – 8192Cleavage; by PL-PROBy similarity
    Active sitei1649 – 16491For PL-PRO activityPROSITE-ProRule annotation
    Active sitei1810 – 18101For PL-PRO activityPROSITE-ProRule annotation
    Sitei3238 – 32392Cleavage; by PL-PROBy similarity
    Active sitei3279 – 32791For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3383 – 33831For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3544 – 35452Cleavage; by 3CL-PROBy similarity
    Sitei3834 – 38352Cleavage; by 3CL-PROBy similarity
    Sitei3917 – 39182Cleavage; by 3CL-PROBy similarity
    Sitei4115 – 41162Cleavage; by 3CL-PROBy similarity
    Sitei4228 – 42292Cleavage; by 3CL-PROBy similarity
    Sitei4367 – 43682Cleavage; by 3CL-PROBy similarity
    Sitei5299 – 53002Cleavage; by 3CL-PROBy similarity
    Sitei5900 – 59012Cleavage; by 3CL-PROBy similarity
    Sitei6427 – 64282Cleavage; by 3CL-PROBy similarity
    Sitei6773 – 67742Cleavage; by 3CL-PROBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1727 – 176438C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4302 – 431817By similarityAdd
    BLAST
    Zinc fingeri4345 – 435814By similarityAdd
    BLAST
    Nucleotide bindingi5581 – 55888ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. helicase activity Source: UniProtKB-KW
    6. methyltransferase activity Source: InterPro
    7. omega peptidase activity Source: InterPro
    8. RNA binding Source: UniProtKB-KW
    9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    10. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    2. induction by virus of host autophagy Source: UniProtKB-KW
    3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    4. suppression by virus of host gene expression Source: UniProtKB-KW
    5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    7. transcription, DNA-templated Source: InterPro
    8. viral protein processing Source: InterPro
    9. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Short name:
    pp1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 15 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    Leader protein
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p65 homolog
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name:
    PL-PRO
    Non-structural protein 4
    Short name:
    nsp4
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    nsp5
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Non-structural protein 8
    Short name:
    nsp8
    Non-structural protein 9
    Short name:
    nsp9
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    nsp12
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    nsp13
    Exoribonuclease (EC:3.1.13.-)
    Short name:
    ExoN
    Alternative name(s):
    nsp14
    Alternative name(s):
    NendoU
    nsp15
    Alternative name(s):
    nsp16
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiBat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
    Taxonomic identifieri349344 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiRhinolophus ferrumequinum (Greater horseshoe bat) [TaxID: 59479]
    Rhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
    Rhinolophus pearsonii [TaxID: 188571]
    Rhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399]
    ProteomesiUP000006570: Genome

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
    Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. host cell membrane Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Non-structural protein 1By similarityPRO_0000043079Add
    BLAST
    Chaini180 – 818639Non-structural protein 2By similarityPRO_0000043080Add
    BLAST
    Chaini819 – 27381920Non-structural protein 3By similarityPRO_0000043081Add
    BLAST
    Chaini2739 – 3238500Non-structural protein 4By similarityPRO_0000283840Add
    BLAST
    Chaini3239 – 35443063C-like proteinaseBy similarityPRO_0000043082Add
    BLAST
    Chaini3545 – 3834290Non-structural protein 6By similarityPRO_0000043083Add
    BLAST
    Chaini3835 – 391783Non-structural protein 7By similarityPRO_0000043084Add
    BLAST
    Chaini3918 – 4115198Non-structural protein 8By similarityPRO_0000043085Add
    BLAST
    Chaini4116 – 4228113Non-structural protein 9By similarityPRO_0000043086Add
    BLAST
    Chaini4229 – 4367139Non-structural protein 10By similarityPRO_0000043087Add
    BLAST
    Chaini4368 – 5299932RNA-directed RNA polymeraseBy similarityPRO_0000043088Add
    BLAST
    Chaini5300 – 5900601HelicaseBy similarityPRO_0000043089Add
    BLAST
    Chaini5901 – 6427527ExoribonucleaseBy similarityPRO_0000043090Add
    BLAST
    Chaini6428 – 6773346Uridylate-specific endoribonucleaseBy similarityPRO_0000043091Add
    BLAST
    Chaini6774 – 7071298Putative 2'-O-methyl transferaseBy similarityPRO_0000043092Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C6W6.
    SMRiP0C6W6. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360, 6428-6771.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2201 – 222121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2312 – 233423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2349 – 236921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2753 – 277321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3020 – 304021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3059 – 307921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3081 – 310121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3103 – 312321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3140 – 316021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3562 – 358221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3584 – 360421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3610 – 363021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3657 – 367620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3683 – 370220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3726 – 374621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3754 – 377421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1001 – 1167167MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1609 – 1873265Peptidase C16PROSITE-ProRule annotationAdd
    BLAST
    Domaini3239 – 3544306Peptidase C30PROSITE-ProRule annotationAdd
    BLAST
    Domaini4979 – 5141163RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini5300 – 538384CV MBDAdd
    BLAST
    Domaini5556 – 5737182(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini5738 – 5907170(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2201 – 2369169HD1By similarityAdd
    BLAST
    Regioni2753 – 3160408HD2By similarityAdd
    BLAST
    Regioni3562 – 3774213HD3By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi930 – 99970Glu-richAdd
    BLAST
    Compositional biasi2208 – 22114Poly-Leu
    Compositional biasi3764 – 37674Poly-Cys

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1727 – 176438C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4302 – 431817By similarityAdd
    BLAST
    Zinc fingeri4345 – 435814By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1ab (identifier: P0C6W6-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MESLVLGINE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKSGT     50
    CGIVELEKGV LPQPEQPYVF IKRSDAQGTD HGHRVRELVA ELDGVQYGRS 100
    GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG 150
    TDPIEDYEQN WNTKHGSGVL RELTRELNGG ALTRYVDNNF CGPDGYPLEC 200
    IKDLLARAGK SMCTLSEQLD YIESKRGVYC CRDHGHEIAW FTERSDKSYE 250
    HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI 300
    RSVYPVATPQ ECNNMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV 350
    SEGPNTCGYL PTNAVVKMPC PACQDPEIGP EHSAADYHNH SNIETRLRKG 400
    GRTRCFGGCV FAYVGCYNKR AYWVPRASAD IGSGHTGITG DNVETLNEDL 450
    LEILSRERVN INIVGDFQLN EEVAIILASF SASTSAFIDT IKSLDYKSFK 500
    TIVESCGNYK VTKGKPIKGA WNIGQHRSVL TPLCGFPSQA AGVIRSIFSR 550
    TLDAANHSIP DLQRAAVTIL DSISEQSLRL VDAMVYTSNL LTNSVIIMAY 600
    VTGGLVQQTS QWLSNLLDTT VEKLRPIFAW IEAKLSAGVE FLKDAWEILK 650
    FLITGVFDIV KGQIQVASDN IKDCVKCFVD VVNKALEMCI DQVTIAGAKL 700
    RSLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDSHDTV 750
    LTSEEVVLKN GELEALETPV DSFTNGAVVG TPVCINGLML LEIKANEQYC 800
    ALSPGLLATN NVFRLKGGAP TKGVTFGEDT VVEVQGYKNV RITFELDERV 850
    DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE 900
    WSVATFYLFD DSGEEKLSSR MYCSFYPPDE EEDCEEYEEE EEVSERTCEH 950
    EYGTEEDYKG LPLEFGASTD IIQVEEQEEE DWLDDAVEAE PEPEPLHEEP 1000
    VNQLTGYLKL TDNVAIKCVD IVEEAQNANP MVIVNAANIH LKHGGGVAGA 1050
    LNKATNGAMQ KESDHYIKLN GPLTVGGSCL LSGHNLAKKC LHVVGPNLNA 1100
    GEDIQLLKAA YENFNSQDIL LAPLLSAGIF GAKPLQSLQM CVQTVRTQVY 1150
    IVVNDKVLYE QVVMDYLDSL KPKVEAPKQE VLPKAEYPKV DEKSVVQKTI 1200
    DVKPKIKACI DEVTTTLEET KFLTNKLLLF TDINGKLYQD SKNMLRGEDM 1250
    SFLEKDAPYM VGDVITSGDI TCVVIPSKKA GGTTEMLSRA LKKVPINEYI 1300
    TTYPGQGCAG YTLEEAKTAL KKCKSAFYVL PSETPNAKEE ILGTVSWNLR 1350
    EMLAHAEETR KLMPVCMDVR AIMATIQRKY KGIKIQEGIV DYGVRFFFYT 1400
    SKEPVASIIT KLNSLNEPLV TMPIGYVTHG FNLEEAARCM RSLKAPAIVS 1450
    VSSPDAVTTY NGYLTSSSKT SEDHFVETVS LAGSYRDWSY SGQRTELGVE 1500
    FLKRGEKIVY HTLESPVKFH LDGEVLPLDK LKSLLSLREV KTIKVFTTVD 1550
    NTNLHTQLVD MSMTYGQQLG PTYLEGADVT KIKPHVNHEG KTFFVLPSDD 1600
    TLRSEAFEYY HTLDESFLGR YMSALNHTKK WKFPQVGGLT SIKWADNNCY 1650
    LSSVLLALQQ IEVKFNAPAL QEAYYRARAG DAANFCALIL AYSNKTVGEL 1700
    GDVRETMTHL LQHANLESAK RVLNVVCKHC GQKTTTLTGV EAVMYMGTLS 1750
    YDNLKMGVSI PCVCGRDATQ YLVQQESSFV MMSAPPAEYK LQQGTFLCAN 1800
    EYTGNYQCGH YTHITAKETL YRIDGAHLTK MSEYKGPVTD VFYKETSYTT 1850
    TIKPVSYKLD GVTYTEIEPK LDGYYKKDNA YYTEQPIDLI PTQPLPNASF 1900
    DNFKLTCSNT KFADDLNQMT GFTKPASREL SVTFFPDLNG DVVAIDYRHY 1950
    SASFKKGAKL LHKPIVWHIN QATTKTTFKP NTWCLRCLWS TKPVDTSNSF 2000
    EVLAVEDTQG MDNLACESQQ PTPEEVVENP TIQKEVIECD VKTTEVVGNV 2050
    ILKPSDEGVK VTQELDHEDL MAAYVENTSI TIKKPNELSL ALGLKTIATH 2100
    GIAAINSVPW GKILAYVKPF LGQAAVTTSN CAKRLVQRMF NNYMPYVLTL 2150
    LFQLCTFTKS TNSRIRASLP TTIAKNSVRG IVRLCLDAGI NYVKSPKFSK 2200
    LFTIAMWLLL LSICLGSLIY VTAALGVLLS NFGAPSYCSG VRESYLNSSN 2250
    VTTMDFCEGS FPCSVCLSGL DSLDSYPALE TIQVTISSYK LDLTILGLAA 2300
    EWFFAYMLFT KFFYLLGLSA IMQVFFGYFA SHFISNSWLM WFIISIVQMA 2350
    PVSAMVRMYI FFASFYYIWK SYVHIMDGCT SSTCMMCYKR NRATRVECTT 2400
    IVNGMKRSFY VYANGGRGFC KTHNWNCLNC DTFCAGSTFI SDEVARDLSL 2450
    QFKRPINPTD QSSYVVDSVA VKNGALHLYF DKAGQKTYER HPLSHFVNLD 2500
    NLRANNTKGS LPINVIVFDG KSKCDESAAK SASVYYSQLM CQPILLLDQA 2550
    LVSDVGDSTE VSVKMFDAYV DTFSATFSVP MEKLKALVAT AHSELAKGVA 2600
    LDGVLSTFVS ASRQGVVDTD VDTKDVIECL KLSHHSDLEV TGDSCNNFML 2650
    TYNKVENMTP RDLGACIDCN ARHINAQVAR SHNVSLIWNV KDYMSLSEQL 2700
    RKQIRSAAKK NNIPFRLTCA TTRQVVNVIT TKISLKGGKI VSTWFKIMLK 2750
    ATLLCVLAAL VCYIVMPVHI LSVHGGYTNE IIGYKAIQDG VTRDIVSTDD 2800
    CFANKHAGFD SWFSQRGGSY KNDKSCPVVA AIITREIGFI VPGLPGTVLR 2850
    AINGDFLHFL PRVFSAVGNI CYTPSKLIEY SDFSTSACVL AAECTIFKDA 2900
    MGKPVPYCYD TNLLEGSISY SELRPDTRYV LMDGSIIQFP NAYLEGSVRV 2950
    VTTFDAEYCR HGTCERSEAG ICLSTSGRWV LNNEHYRALP GVFCGVDAMN 3000
    LIANIFTPLV QPVGALDVSA SVVAGGIIAI LVTCAAYYFM KFRRAFGEYN 3050
    HVVAANAPLF LMSFTILCLA PAYSFLPGVY SVFYLYLTFY FTNDVSFLAH 3100
    LQWFAMFSPI VPFWITAIYV FCISLKHFHW FFNNYLRKRV VFNGVTFSTF 3150
    EEAALCTFLL NKEMYLKLRS ETLLPLTQYN RYLALYNKYK YFSGALDTTS 3200
    YREAACCHLA KALNDFSNSG ADVLYQPPQT SITSAVLQSG FRKMAFPSGK 3250
    VEGCMVQVTC GTTTLNGLWL DDTVYCPRHV ICTAEDMLNP NYEDLLIRKS 3300
    NHSFLVQAGN VQLRVIGHSM QNCLLRLKVD TSNPKTPKYK FVRIQPGQTF 3350
    SVLACYNGSP SGVYQCAMRP NHTIKGSFLN GSCGSVGFNI DYDCVSFCYM 3400
    HHMELPTEVH AGTDLEGKFY GPFVDRQTAQ AAGTDTTITL NVLAWLYAAV 3450
    INGDRWFLNR FTTTLNDFNL VAMKYNYEPL TQDHVDILGP LSAQTGIAVL 3500
    DMCAALKELL QNGMNGRTIL GSTILEDEFT PFDVVRQCSG VTFQGKFKRI 3550
    VKGTHHWMLL TFLTSLLILV QSTQWSLFFF VYENAFLPFT LGIMAVAACA 3600
    MLLVKHKHAF LCLFLLPSLA TVAYFNMVYM PASWVMRIMT WLELADTSLS 3650
    GYRLKDCVMY ASALVLLVLM TARTVYDDAA RRVWTLMNVI TLVYKVYYGN 3700
    ALDQAISMWA LVISVTSNYS GVVTTIMFLA RAIVFVCVEY YPLLFITGNT 3750
    LQCIMLVYCF LGYCCCCYFG LFCLLNRYFR LTLGVYDYLV STQEFRYMNS 3800
    QGLLPPKSSI DAFKLNIKLL GIGGKPCIKV ATVQSKMSDV KCTSVVLLSV 3850
    LQQLRVESSS KLWAQCVQLH NDILLAKDTT EAFEKMVSLL SVLLSMQGAV 3900
    DINKLCEEML DNRATLQAIA SEFSSLPSYA AYATAQEAYE QAVANGDSEV 3950
    VLKKLKKSLN VAKSEFDRNA AMQRKLEKMA DQAMTQMYKQ ARSEDKRAKV 4000
    TSAMQTMLFT MLRKLDNDAL NNIINNARDG CVPLNIIPLT TAAKLMVVVP 4050
    DYGTYKNTCD GNTFTYASAL WEIQQVVDAD SKIVQLSEIN MENSSNLAWP 4100
    LIVTALRANS AVKLQNNELS PVALRQMSCA AGTTQTACTD DNALAYYNNS 4150
    KGGRFVLALL SDHQDLKWAR FPKSDGTGTI YTELEPPCRF VTDTPKGPKV 4200
    KYLHFIKGLN NLNRGMVLGS LAATVRLQAG NATEVPANST VLSFCAFAVD 4250
    PAKAYKDYLA SGGQPITNCV KMLCTHTGTG QAITVTPEAN MDQESFGGAS 4300
    CCLYCRCHID HPNPKGFCDL KGKYVQIPTT CANDPVGFTL RNTVCTVCGM 4350
    WKGYGCSCDQ LREPMMQSAD ASTFLNRVCG VSAARLTPCG TGISTDVVYR 4400
    AFDIYNEKVA GFAKFLKTNC CRFQEKDEEG NLLDSYFVVK RHTMSNYQHE 4450
    ETIYNLVKDC PAVAVHDFFK FRVDGDMVPH ISRQRLTKYT MADLVYALRH 4500
    FDEGNCDTLK EILVTYNCCD DDYFNKKDWY DFVENPDILR VYANLGERVR 4550
    QALLKTVQFC DAMRDAGIVG VLTLDNQDLN GNWYDFGDFV QVAPGCGVPI 4600
    VDSYYSLLMP ILTLTRALAA ESHMDADLAK PLIKWDLLKY DFTAERLCLF 4650
    DRYFKYWDQT YHPNCINCLD DRCILHCANF NVLFSTVFPP TSFGPLVRKI 4700
    FVDGVPFVVS TGYHFRELGV VHNQDVNLHS SRLSFKELLV YAADPAMHAA 4750
    SGNLLLDKRT TCFSVAALTN NVAFQTVKPG NFNKDFYDFA VSKGFFKEGS 4800
    SVELKHFFFA QDGNAAISDY DYYRYNLPTM CDIRQLLFVV EVVDKYFDCY 4850
    DGGCINANQV IVNNLDKSAG FPFNKWGKAR LYYDSMSYED QDALFAYTKR 4900
    NVIPTITQMN LKYAISAKNR ARTVAGVSIC STMTNRQFHQ KLLKSIAATR 4950
    GATVVIGTSK FYGGWHNMLK TVYSDVETPH LMGWDYPKCD RAMPNMLRIM 5000
    ASLVLARKHS TCCNLSHRFY RLANECAQVL SEMVMCGGSL YVKPGGTSSG 5050
    DATTAYANSV FNICQAVTAN VNALLSTDGN KIADKYVRNL QHRLYECLYR 5100
    NRDVDHEFVG EFYAYLRKHF SMMILSDDAV VCYNSNYAAQ GLVASIKNFK 5150
    AVLYYQNNVF MSEAKCWTET DLTKGPHEFC SQHTMLVKQG DDYVYLPYPD 5200
    PSRILGAGCF VDDIVKTDGT LMIERFVSLA IDAYPLTKHP NQEYADVFHL 5250
    YLQYIRKLHD ELTGHMLDMY SVMLTNDNTS RYWEPEFYEA MYTPHTVLQA 5300
    VGACVLCNSQ TSLRCGACIR RPFLCCKCCY DHVISTSHKL VLSVNPYVCN 5350
    APGCDVTDVT QLYLGGMSYY CKSHKPPISF PLCANGQVFG LYKNTCVGSD 5400
    NVTDFNAIAT CDWTNAGDYI LANTCTERLK LFAAETLKAT EETFKLSYGI 5450
    ATVREVLSDR ELHLSWEVGK PRPPLNRNYV FTGYRVTKNS KVQIGEYTFE 5500
    KGDYGDAVVY RGTTTYKLNI GDYFVLTSHT VMPLSAPTLV PQEHYVRITG 5550
    LYPTLNISDE FSSNVANYQK VGMQKYSTLQ GPPGTGKSHF AIGLALYYPS 5600
    ARIVYTACSH AAVDALCEKA LKYLPIDKCS RIIPARARVE CFDKFKVNST 5650
    LEQYVFCTVN ALPETTADIV VFDEISMATN YDLSVVNARL RAKHYVYIGD 5700
    PAQLPAPRTL LTKGTLEPEY FNSVCRLMKT IGPDMFLGTC RRCPAEIVDT 5750
    VSALVYDNKL KAHKEKSAQC FKMFYKGVIT HDVSSAINRP QIGVVREFLT 5800
    RNPAWRKAVF ISPYNSQNAV ASKILGLPTQ TVDSSQGSEY DYVIFTQTTE 5850
    TAHSCNVNRF NVAITRAKIG ILCIMSDRDL YDKLQFTSLE VPRRNVATLQ 5900
    AENVTGLFKD CSKIITGLHP TQAPTHLSVD TKFKTEGLCV DIPGIPKDMT 5950
    YRRLISMMGF KMNYQVNGYP NMFITREEAI RHVRAWIGFD VEGCHATRDA 6000
    VGTNLPLQLG FSTGVNLVAI PTGYVDTENN TEFTRVNAKP PPGDQFKHLI 6050
    PLMYKGLPWN VVRIKIVQML SDTLKGLSDR VVFVLWAHGF ELTSMKYFVK 6100
    IGPERTCCLC DKRATCFSTS SDTYACWNHS VGFDYVYNPF MIDVQQWGFT 6150
    GNLQSNHDQH CQVHGNAHVA SCDAIMTRCL AVHECFVKRV DWSVEYPIIG 6200
    DELKINSACR KVQHMVVKSA LLADKFPVLH DIGNPKAIKC VPQAEVEWKF 6250
    YDAQPCSDKA YKIEELFYSY ATHHDKFTDG VCLFWNCNVD RYPANAIVCR 6300
    FDTRVLSNLN LPGCDGGSLY VNKHAFHTPA FDKSAFTNLK QLPFFYYSDS 6350
    PCESHGKQVV SDIDYVPLKS ATCITRCNLG GAVCRHHANE YRQYLDAYNM 6400
    MISAGFSLWI YKQFDTYNLW NTFTRLQSLE NVAYNVVNKG HFDGQAGETP 6450
    VSIINNAVYT KVDGFDVEIF ENKTTLPVNV AFELWAKRNI KSVPEIKILN 6500
    NLGVDIAANT VIWDHKREAP VHMSTIGVCT MTDIAKKPTE SACSSLTVLF 6550
    DGRVEGQVDL FRNARNGVLI TEGSVKGLTP SKGPAQASVN GVTLIGESVK 6600
    TQFNYFKKVD GIIQQLPETY FTQSRDLEDF KPRSQMETDF LELAMDEFIQ 6650
    RYKLEGYAFE HIVYGDFSHG QLGGLHLMIG LAKRSRDSPL KLEDFIPMDS 6700
    TVKNYFITDA QTGSSKCVCS VIDLLLDDFV EIIKSQDLSV VSKVVKVTID 6750
    YAEISFMLWC KDGHVETFYP KLQASQAWQP GVAMPNLYKM QRMLLEKCDL 6800
    QNYGENAVIP KGIMMNVAKY TQLCQYLNTL TLAVPYNMRV IHFGAGSDKG 6850
    VAPGTAVLRQ WLPTGTLLVD SDLNDFVSDA DSTLIGDCAT VHTANKWDLI 6900
    VSDMYDPKAK HVTKENDSKE GFFTYLCGFI KQKLALGGSV AVKITEHSWN 6950
    ADLYKLMGHF SWWTAFVTNV NASSSEAFLI GVNYLGKPKE QIDGYTMHAN 7000
    YIFWRNTNPI QLSSYSLFDM SKFPLKLRGT AVMSLKENQI NDMIYSLLEK 7050
    GRLIIRENNR VVVSSDILVN N 7071

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:7,071
    Mass (Da):790,103
    Last modified:June 10, 2008 - v1
    Checksum:i711112FAF32354AB
    GO
    Isoform Replicase polyprotein 1a (identifier: P0C6T7-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform can be found in the external entry P0C6T7.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by conventional translation.

    Length:4,380
    Mass (Da):486,337
    GO

    Sequence cautioni

    The sequence AAZ67050.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAZ67051.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ071615 Genomic RNA. Translation: AAZ67050.1. Sequence problems.
    DQ071615 Genomic RNA. Translation: AAZ67051.1. Sequence problems.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ071615 Genomic RNA. Translation: AAZ67050.1 . Sequence problems.
    DQ071615 Genomic RNA. Translation: AAZ67051.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali P0C6W6.
    SMRi P0C6W6. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360, 6428-6771.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF06478. Corona_RPol_N. 1 hit.
    PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEi PS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiR1AB_BCRP3
    AccessioniPrimary (citable) accession number: P0C6W6
    Secondary accession number(s): Q3I5J6, Q3I5J7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3