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P0C6W5

- R1AB_BCHK9

UniProt

P0C6W5 - R1AB_BCHK9

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Protein

Replicase polyprotein 1ab

Gene
rep, 1a-1b
Organism
Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.1 Publication
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.1 Publication
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.1 Publication
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.1 Publication
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.1 Publication
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.1 Publication
Nsp9 is a ssRNA-binding protein By similarity.1 Publication
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.1 Publication
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei175 – 1762Cleavage Reviewed prediction
Sitei772 – 7732Cleavage; by PL-PRO Reviewed prediction
Active sitei1533 – 15331For PL-PRO activity By similarity
Active sitei1694 – 16941For PL-PRO activity By similarity
Sitei2609 – 26102Cleavage; by PL-PRO Reviewed prediction
Sitei3103 – 31042Cleavage; by 3CL-PRO Reviewed prediction
Active sitei3144 – 31441For 3CL-PRO activity By similarity
Active sitei3248 – 32481For 3CL-PRO activity By similarity
Sitei3409 – 34102Cleavage; by 3CL-PRO Reviewed prediction
Sitei3699 – 37002Cleavage; by 3CL-PRO Reviewed prediction
Sitei3782 – 37832Cleavage; by 3CL-PRO Reviewed prediction
Sitei3982 – 39832Cleavage; by 3CL-PRO Reviewed prediction
Sitei4094 – 40952Cleavage; by 3CL-PRO Reviewed prediction
Sitei4233 – 42342Cleavage; by 3CL-PRO Reviewed prediction
Sitei5165 – 51662Cleavage; by 3CL-PRO Reviewed prediction
Sitei5766 – 57672Cleavage; by 3CL-PRO Reviewed prediction
Sitei6296 – 62972Cleavage; by 3CL-PRO Reviewed prediction
Sitei6633 – 66342Cleavage; by 3CL-PRO Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1610 – 164738C4-type By similarityAdd
BLAST
Zinc fingeri4168 – 418417 By similarityAdd
BLAST
Zinc fingeri4211 – 422414 By similarityAdd
BLAST
Nucleotide bindingi5447 – 54548ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. methyltransferase activity Source: InterPro
  7. omega peptidase activity Source: InterPro
  8. RNA binding Source: UniProtKB-KW
  9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  5. suppression by virus of host translation Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral protein processing Source: InterPro
  9. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus HKU9 (BtCoV) (BtCoV/HKU9)
Taxonomic identifieri694006 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRousettus leschenaultii (Leschenault's rousette) [TaxID: 9408]
ProteomesiUP000006576: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2015 – 203521Helical; Reviewed predictionAdd
BLAST
Transmembranei2040 – 206021Helical; Reviewed predictionAdd
BLAST
Transmembranei2081 – 210121Helical; Reviewed predictionAdd
BLAST
Transmembranei2162 – 218221Helical; Reviewed predictionAdd
BLAST
Transmembranei2183 – 220321Helical; Reviewed predictionAdd
BLAST
Transmembranei2218 – 223821Helical; Reviewed predictionAdd
BLAST
Transmembranei2621 – 264121Helical; Reviewed predictionAdd
BLAST
Transmembranei2719 – 273921Helical; Reviewed predictionAdd
BLAST
Transmembranei2865 – 288521Helical; Reviewed predictionAdd
BLAST
Transmembranei2887 – 290721Helical; Reviewed predictionAdd
BLAST
Transmembranei2916 – 293621Helical; Reviewed predictionAdd
BLAST
Transmembranei2946 – 296621Helical; Reviewed predictionAdd
BLAST
Transmembranei2970 – 299021Helical; Reviewed predictionAdd
BLAST
Transmembranei3423 – 344321Helical; Reviewed predictionAdd
BLAST
Transmembranei3449 – 346921Helical; Reviewed predictionAdd
BLAST
Transmembranei3474 – 349421Helical; Reviewed predictionAdd
BLAST
Transmembranei3517 – 353721Helical; Reviewed predictionAdd
BLAST
Transmembranei3569 – 358921Helical; Reviewed predictionAdd
BLAST
Transmembranei3592 – 361221Helical; Reviewed predictionAdd
BLAST
Transmembranei3620 – 364021Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Non-structural protein 1 Reviewed predictionPRO_0000291350Add
BLAST
Chaini176 – 772597Non-structural protein 2 Reviewed predictionPRO_0000291351Add
BLAST
Chaini773 – 26091837Non-structural protein 3 Reviewed predictionPRO_0000291352Add
BLAST
Chaini2610 – 3103494Non-structural protein 4 Reviewed predictionPRO_0000291353Add
BLAST
Chaini3104 – 34093063C-like proteinase Reviewed predictionPRO_0000291354Add
BLAST
Chaini3410 – 3699290Non-structural protein 6 Reviewed predictionPRO_0000291355Add
BLAST
Chaini3700 – 378283Non-structural protein 7 Reviewed predictionPRO_0000291356Add
BLAST
Chaini3783 – 3982200Non-structural protein 8 Reviewed predictionPRO_0000291357Add
BLAST
Chaini3983 – 4094112Non-structural protein 9 Reviewed predictionPRO_0000291358Add
BLAST
Chaini4095 – 4233139Non-structural protein 10 Reviewed predictionPRO_0000291359Add
BLAST
Chaini4234 – 5165932RNA-directed RNA polymerase Reviewed predictionPRO_0000291360Add
BLAST
Chaini5166 – 5766601Helicase Reviewed predictionPRO_0000291361Add
BLAST
Chaini5767 – 6296530Exoribonuclease Reviewed predictionPRO_0000291362Add
BLAST
Chaini6297 – 6633337Uridylate-specific endoribonuclease Reviewed predictionPRO_0000291363Add
BLAST
Chaini6634 – 6930297Putative 2'-O-methyl transferase Reviewed predictionPRO_0000291364Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Proteomic databases

PRIDEiP0C6W5.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6W5.
SMRiP0C6W5. Positions 934-1096, 3106-3403, 3699-3782, 3820-3972, 3985-4094, 4104-4224.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini930 – 1097168MacroAdd
BLAST
Domaini1492 – 1757266Peptidase C16Add
BLAST
Domaini3104 – 3409306Peptidase C30Add
BLAST
Domaini4845 – 5007163RdRp catalyticAdd
BLAST
Domaini5166 – 524984CV MBDAdd
BLAST
Domaini5412 – 5603192(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini5604 – 5778175(+)RNA virus helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2015 – 2238224HD1 By similarityAdd
BLAST
Regioni2621 – 2990370HD2 By similarityAdd
BLAST
Regioni3423 – 3640218HD3 By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5170 – 519526Cys-richAdd
BLAST
Compositional biasi6832 – 68354Poly-Ser

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1610 – 164738C4-type By similarityAdd
BLAST
Zinc fingeri4168 – 418417 By similarityAdd
BLAST
Zinc fingeri4211 – 422414 By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W5-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MEGVPDPPKL KSMVVTTLKW CDPFANPNVT GWDIPIEEAL EYAKQQLRTP     50
EPQLVFVPYY LSHAPGISGD RVVITDSIWY ATNFGWQPIR ELAMDKDGVR 100
YGRGGTHGVL LPMQDPSFIM GDIDIQIRKY GIGANSPPDV LPLWDGFSDP 150
GPDVGPYLDF PDNCCPTKPK AKRGGDVYLS DQYGFDNNGI LVEPVMKLLG 200
VIKSDFTLEQ LLAALGKYRT EDGYDLPDGY VKVAIKVGRK AVPVLKQSIF 250
TVVGVTEQLV PGYYYPFSTS SVVEHTKPTR GGPVGKTVEA VMLSLYGTNN 300
YNPATPVARL KCSYCDYYGW TPLKDIGTVN CLCGAEFQLT SSCVDAESAG 350
VIKPGCVMLL DKSPGMRLIP GNRTYVSFGG AIWSPIGKVN GVTVWVPRAY 400
SIVAGEHSGA VGSGDTVAIN KELVEYLIEG IRVDADTLDN PTCATFIANL 450
DCDTKAPVVH TVESLQGLCL ANKIMLGDKP LPTDEFHPFI VGLAYHVQRA 500
CWYGALASRT FEAFRDFVRT EEERFAQFFG KVCAPINGCV YLAYTTGRVT 550
LFSAYQVLNT AIAKSKDAFG GVAAIVVDML KPILEWVLKK MSIAKGAWLP 600
YAEGLLALFK AQFTVVKGKF QFLRASLNSK CHSLCDLLTT IMSKLLTSVK 650
WAGCKVDALY TGTYYYFSRK GVLTEVQLCA KRLGLLLTPK QQKMEVEVLD 700
GDFDAPVTLT DLELEECTGV LEEVFGASDV KLVKGTLVSL ASKLFVRTED 750
GFLYRYVKSG GVLGKAFRLR GGGVSKVTFG DEEVHTIPNT VTVNFSYDVC 800
EGLDAILDKV MAPFQVEEGT KLEDLACVVQ KAVYERLSDL FSDCPAELRP 850
INLEDFLTSE CFVYSKDYEK ILMPEMYFSL EDAVPVDDEM VDDIEDTVEQ 900
ASDSDDQWLG DEGAEDCDNT IQDVDVATSM TTPCGYTKIA EHVYIKCADI 950
VQEARNYSYA VLVNAANVNL HHGGGVAGAL NRATNNAMQK ESSEYIKANG 1000
SLQPGGHVLL SSHGLASHGI LHVVGPDKRL GQDLALLDAV YAAYTGFDSV 1050
LTPLVSAGIF GFTVEESLCS LVKNVACTTY VVVYDRQLYE RALATSFDVP 1100
GPQSSVQHVP AIDWAEAVEV QESIVDQVET PSLGAVDTVD SNADSGLNET 1150
ARSPENVVGS VPDDVVADVE SCVRDLVRQV VKKVKRDKRP PPIVPQQTVE 1200
QQPQEISSPG DCNTVLVDVV SMSFSAMVNF GKEKGLLIPV VIDYPAFLKV 1250
LKRFSPKEGL FSSNGYEFYG YSRDKPLHEV SKDLNSLGRP LIMIPFGFIV 1300
NGQTLAVSAV SMRGLTVPHT VVVPSESSVP LYRAYFNGVF SGDTTAVQDF 1350
VVDILLNGAR DWDVLQTTCT VDRKVYKTIC KRGNTYLCFD DTNLYAITGD 1400
VVLKFATVSK ARAYLETKLC APEPLIKVLT TVDGINYSTV LVSTAQSYRA 1450
QIGTVFCDGH DWSNKNPMPT DEGTHLYKQD NFSSAEVTAI REYYGVDDSN 1500
IIARAMSIRK TVQTWPYTVV DGRVLLAQRD SNCYLNVAIS LLQDIDVSFS 1550
TPWVCRAYDA LKGGNPLPMA EVLIALGKAT PGVSDDAHMV LSAVLNHGTV 1600
TARRVMQTVC EHCGVSQMVF TGTDACTFYG SVVLDDLYAP VSVVCQCGRP 1650
AIRYVSEQKS PWLLMSCTPT QVPLDTSGIW KTAIVFRGPV TAGHYMYAVN 1700
GTLISVYDAN TRRRTSDLKL PATDILYGPT SFTSDSKVET YYLDGVKRTT 1750
IDPDFSKYVK RGDYYFTTAP IEVVAAPKLV TSYDGFYLSS CQNPQLAESF 1800
NKAINATKTG PMKLLTMYPN VAGDVVAISD DNVVAHPYGS LHMGKPVLFV 1850
TRPNTWKKLV PLLSTVVVNT PNTYDVLAVD PLPVNNETSE EPISVKAPIP 1900
LYGLKATMVL NGTTYVPGNK GHLLCLKEFT LTDLQTFYVE GVQPFVLLKA 1950
SHLSKVLGLR VSDSSLHVNH LSKGVVYAYA ATRLTTRVTT SLLGGLVTRS 2000
VRKTADFVRS TNPGSKCVGL LCLFYQLFMR FWLLVKKPPI VKVSGIIAYN 2050
TGCGVTTCVL NYLRSRCGNI SWSRLLKLLR YMLYIWFVWT CLTICGVWLS 2100
EPYAPSLVTR FKYFLGIVMP CDYVLVNETG TGWLHHLCMA GMDSLDYPAL 2150
RMQQHRYGSP YNYTYILMLL EAFFAYLLYT PALPIVGILA VLHLIVLYLP 2200
IPLGNSWLVV FLYYIIRLVP FTSMLRMYIV IAFLWLCYKG FLHVRYGCNN 2250
VACLMCYKKN VAKRIECSTV VNGVKRMFYV NANGGTHFCT KHNWNCVSCD 2300
TYTVDSTFIC RQVALDLSAQ FKRPIIHTDE AYYEVTSVEV RNGYVYCYFE 2350
SDGQRSYERF PMDAFTNVSK LHYSELKGAA PAFNVLVFDA TNRIEENAVK 2400
TAAIYYAQLA CKPILLVDKR MVGVVGDDAT IARAMFEAYA QNYLLKYSIA 2450
MDKVKHLYST ALQQISSGMT VESVLKVFVG STRAEAKDLE SDVDTNDLVS 2500
CIRLCHQEGW EWTTDSWNNL VPTYIKQDTL STLEVGQFMT ANAKYVNANI 2550
AKGAAVNLIW RYADFIKLSE SMRRQLKVAA RKTGLNLLVT TSSLKADVPC 2600
MVTPFKIIGG HRRIVSWRRV LIHVFMLLVV LNPQWFTPWY IMRPIEYNVV 2650
DFKVIDNAVI RDITSADQCF ANKFSAFENW YSNRYGSYVN SRGCPMVVGV 2700
VSDIVGSLVP GLPARFLRVG TTLLPLVNYG LGAVGSVCYT PHYAINYDVF 2750
DTSACVLAAT CTLFSSASGE RMPYCADAAL IQNASRYDML KPHVMYPFYE 2800
HSGYIRFPEV ISAGVHIVRT MAMEYCKVGR CDVSEAGLCM SLQPRWVVNN 2850
AYFRQQSGVY CGTSAFDLFM NMLLPIFTPV GAVDITTSIL MGALLAVVVS 2900
MSLYYLLRFR RAFGDYSGVI FTNILAFVLN VIVLCLEGPY PMLPSIYAMV 2950
FLYATCYFGS DIACMMHVSF LIMFAGVVPL WVTVLYIVVV LSRHILWFAS 3000
LCTKRTVQVG DLAFHSFQDA ALQTFMLDKE VFLRLKREIS SDAYFKYLAM 3050
YNKYKYYSGP MDTAAYREAA CSHLVMALEK YSNGGGDTIY QPPRCSVASA 3100
ALQAGLTRMA HPSGLVEPCL VKVNYGSMTL NGIWLDNFVI CPRHVMCSRD 3150
ELANPDYPRL SMRAANYDFH VSQNGHNIRV IGHTMEGSLL KLTVDVNNPK 3200
TPAYSFIRVS TGQAMSLLAC YDGLPTGVYT CTLRSNGTMR ASFLCGSCGS 3250
PGFVMNGKEV QFCYLHQLEL PNGTHTGTDF SGVFYGPFED KQVPQLAAPD 3300
CTITVNVLAW LYAAVLSGEN WFLTKSSISP AEFNNCAVKY MCQSVTSESL 3350
QVLQPLAAKT GISVERMLSA LKVLLSAGFC GRTIMGSCSL EDEHTPYDIG 3400
RQMLGVKLQG KFQSMFRWTL QWFAIIFVLT ILILLQLAQW TFVGALPFTL 3450
LLPLIGFVAV CVGFVSLLIK HKHTYLTVYL LPVAMVTAYY NFQYTPEGVQ 3500
GYLLSLYNYV NPGRIDVIGT DLLTMLIISV ACTLLSVRMV RTDAYSRIWY 3550
VCTAVGWLYN CWTGSADTVA ISYLTFMVSV FTNYTGVACA SLYAAQFMVW 3600
VLKFLDPTIL LLYGRFRCVL VCYLLVGYLC TCYFGVFNLI NRLFRCTLGN 3650
YEYVVSSQEL RYMNSHGLLP PTNSWQALML NIKLAGIGGI PIYRVSTIQS 3700
NMTDLKCTSV VLLSVLQQLR VESSSKLWAL CVKLHNEILA SNSPTEAFEA 3750
FVSLLSVLLS LPGAINLDEL CSSILENNSV LQAVASEFSN LSSYVDYENA 3800
QKAYDTAVAT GAPASTVNAL KKAMNVAKSV LDKDVATTRK LERMSELAMT 3850
AMYKQARAED RRSKVTAAMQ TMLFNMIRRL DSDALSNILN NARNGVVPLG 3900
VIPRTAANKL LLVVPDFSVY TATITMPTLT YAGSAWDVMQ VADADGKTVN 3950
ATDITRENSV NLAWPLVVTA QRQQATSPVK LQNNELMPQT VKRMNVVAGV 4000
SQTACVTDAV AYYNATKEGR HVMAILADTD GLAFAKVEKS TGDGFVILEL 4050
EPPCKFMVDT PKGPALKYLY FTKGLKNLCR GTVLGTLACT VRLHAGSATE 4100
VASNSSILSL CSFSVDPEAT YKDYLDNGGS PIGNCVKMLT PHTGTGLAIT 4150
AKPDANIDQE SFGGASCCLY CRCHIEHPGA SGVCKYKGKF VQIPLVGVND 4200
PIGFCIRNVV CAVCNMWQGY GCPCSSLREI NLQARDECFL NRVRGTSGVA 4250
RLVPLGSGVQ PDIVLRAFDI CNTKVAGFGL HLKNNCCRYQ ELDADGTQLD 4300
SYFVVKRHTE SNYLLEQRCY EKLKDCGVVA RHDFFKFNIE GVMTPHVSRE 4350
RLTKYTMADL VYSLRHFDNN NCDTLKEILV LRGCCTADYF DRKDWYDPVE 4400
NPDIIRVYHN LGETVRKAVL SAVKMADSMV EQGLIGVLTL DNQDLNGQWY 4450
DFGDFIEGPA GAGVAVMDTY YSLAMPVYTM TNMLAAECHV DGDFSKPKRV 4500
WDICKYDYTQ FKYSLFSKYF KYWDMQYHPN CVACADDRCI LHCANFNILF 4550
SMVLPNTSFG PLVQKIYVDG VPFVVSTGYH YRELGVVMNQ DIRQHAQRLS 4600
LRELLVYAAD PAMHVAASNA LADKRTVCMS VAAMTTGVTF QTVKPGQFNE 4650
DFYNFAVKCG FFKEGSTISF KHFFYAQDGN AAISDYDYYR YNLPTMCDIK 4700
QLLFSLEVVD KYFDCYDGGC LQASQVVVAN YDKSAGFPFN KFGKARLYYE 4750
SLSYADQDEL FAYTKRNVLP TITQMNLKYA ISAKNRARTV AGVSIASTMT 4800
NRQFHQKMLK SIAAARGASV VIGTTKFYGG WNRMLRTLCE GVENPHLMGW 4850
DYPKCDRAMP NLLRIFASLI LARKHATCCN ASERFYRLAN ECAQVLSEMV 4900
LCGGGFYVKP GGTSSGDSTT AYANSVFNIC QAVSANLNTF LSIDGNKIYT 4950
TYVQELQRRL YLGIYRSNTV DNELVLDYYN YLRKHFSMMI LSDDGVVCYN 5000
ADYAQKGYVA DIQGFKELLY FQNNVFMSES KCWVEPDITK GPHEFCSQHT 5050
MLVDMKGEQV YLPYPDPSRI LGAGCFVDDL LKTDGTLMME RYVSLAIDAY 5100
PLTKHPDPEY QNVFWCYLQY IKKLHEELTG HLLDTYSVML ASDNASKYWE 5150
VEFYENMYME SATLQSVGTC VVCNSQTSLR CGGCIRRPFL CCKCCYDHVV 5200
STTHKLVLSV TPYVCNNPSC DVADVTQLYL GGMSYYCRDH RPPISFPLCA 5250
NGQVFGLYKN ICTGSPDVAD FNSLATCDWS NSKDYVLANT ATERLKLFAA 5300
ETLRATEENA KQAYASAVVK EVLSDRELVL SWETGKTRPP LNRNYVFTGF 5350
HITKNSKVQL GEYIFEKGDY GDVVNYRSST TYKLQVGDYF VLTSHSVQPL 5400
SSPTLLPQER YTKLVGLYPA MNVPESFASN VVHYQRVGMS RYTTVQGPPG 5450
TGKSHLSIGL ALYYPSAKIV YTACSHAAVD ALCEKAHKNL PINRCSRIVP 5500
AKARVECFSK FKVNDVGAQY VFSTINALPE TTADILVVDE VSMCTNYDLS 5550
MINARVRAKH IVYVGDPAQL PAPRTLLTKG TLAPEHFNSV CRLMVAVGPD 5600
IFLATCYRCP KEIVDTVSAL VYDKKLKANK VTTGECYKCY YKGSVTHDSS 5650
SAINKPQLGL VKEFLIKNPK WQSAVFISPY NSQNSVARRM LGLQTQTVDS 5700
SQGSEFDYVI YCQTSDTAHA LNVNRFNVAI TRAKKGILCV MSDSTLYESL 5750
EFTPLDVNDY VKPKMQSEVT VGLFKDCAKA EPLGPAYAPT FVSVNDKFKL 5800
NESLCVHFDT TELQMPYNRL ISKMGFKFDL NIPGYSKLFI TREQAIREVR 5850
GWVGFDVEGA HACGPNIGTN LPLQIGFSTG VNFVVTPSGY IDTESGSRLA 5900
NVVSKAPPGD QFKHLIPLMR KGEPWSVVRK RIVEMLCDTL DGVSDTVTFV 5950
TWAHGFELTT LHYFAKVGPE RKCFMCPRRA TLFSSVYGAY SCWSHHRHIG 6000
GADFVYNPFL VDVQQWGYVG NLQVNHDNVC DVHKGAHVAS CDAIMTRCLA 6050
IHDCFCGEVN WDVEYPIIAN ELAINRACRS VQRVVLKAAV KALHIETIYD 6100
IGNPKAIKVY GVNVNNWNFY DTNPVVEGVK QLHYVYDVHR DQFKDGLAMF 6150
WNCNVDCYPH NALVCRFDTR VLSKLNLAGC NGGSLYVNQH AFHTDAFNKN 6200
AFVNLKPLPF FYYSDTACEN ATGVSTNYVS EVDYVPLKSN VCITRCNLGG 6250
AVCKKHADEY RNFLESYNTM VSAGFTLWVD KTFDVFNLWS TFVKLQSLEN 6300
VAYNVLKSGH FTAVAGELPV AILNDRLYIK EDGADKLLFT NNTCLPTNVA 6350
FELWAKRSVN VVPEVKLLRN LGVTCTYNLV IWDYESNAPL VPNTVGICTY 6400
TDLTKLDDQV VLVDGRQLDA YSKFCQLKNA IYFSPSKPKC VCTRGPTHAS 6450
INGVVVEAPD RGTAFWYAMR KDGAFVQPTD GYFTQSRTVD DFQPRTQLEI 6500
DFLDLEQSCF LDKYDLHDLG LEHIVYGQFD GTIGGLHLLI GAVRRKRTAH 6550
LVMETVLGTD TVTSYAVIDQ PTASSKQVCS VVDIILDDFI ALIKAQDRSV 6600
VSKVVQCCLD FKVFRFMLWC KGGKISTFYP QLQAKQDWKP GYSMPALYKV 6650
QNAVLEPCLL HNYGQAARLP SGTLMNVAKY TQLCQYLNTC SLAVPAKMRV 6700
MHFGAGSDKG VCPGTAVLKQ WLPADAYLVD NDLCYCASDA DSTYVGSCET 6750
FFSVNKWDFI FSDMYDARTK NTSGDNTSKE GFFTYLTGFI RSKLALGGSI 6800
AIKITEHSWS ADLYAIMGHF NWWTCFCTSV NSSSSEAFLI GVNYIGVGAL 6850
LDGWQMHANY VFWRNSTVMQ LSSYSLYDLQ RFPLRLKGTP VMSLKEDQLN 6900
ELVLNLIRAG RLIVRDAVDI GVRGVACSGV 6930

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:6,930
Mass (Da):769,729
Last modified:June 10, 2008 - v1
Checksum:i9ED06D0888C7EC7B
GO
Isoform Replicase polyprotein 1a (identifier: P0C6T6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6T6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,248
Mass (Da):468,590
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF065513 Genomic RNA. Translation: ABN10910.1.
RefSeqiYP_001039970.1. NC_009021.1. [P0C6W5-1]

Genome annotation databases

GeneIDi4836014.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF065513 Genomic RNA. Translation: ABN10910.1 .
RefSeqi YP_001039970.1. NC_009021.1. [P0C6W5-1 ]

3D structure databases

ProteinModelPortali P0C6W5.
SMRi P0C6W5. Positions 934-1096, 3106-3403, 3699-3782, 3820-3972, 3985-4094, 4104-4224.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0C6W5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4836014.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features."
    Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y.
    J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HKU9-1.
  2. "Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses."
    Tohya Y., Narayanan K., Kamitani W., Huang C., Lokugamage K., Makino S.
    J. Virol. 83:5282-5288(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF NSP1.

Entry informationi

Entry nameiR1AB_BCHK9
AccessioniPrimary (citable) accession number: P0C6W5
Secondary accession number(s): A3EXG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi