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P0C6W5 (R1AB_BCHK9) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    Leader protein
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65 homolog
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name=PL-PRO
  4. Non-structural protein 4
    Short name=nsp4
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    nsp5
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
  8. Non-structural protein 8
    Short name=nsp8
  9. Non-structural protein 9
    Short name=nsp9
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismBat coronavirus HKU9 (BtCoV) (BtCoV/HKU9) [Complete proteome]
Taxonomic identifier694006 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostRousettus leschenaultii (Leschenault's rousette) [TaxID: 9408]

Protein attributes

Sequence length6930 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity. Ref.2

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity. Ref.2

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity. Ref.2

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity. Ref.2

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity. Ref.2

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity. Ref.2

Nsp9 is a ssRNA-binding protein By similarity. Ref.2

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity. Ref.2

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response. Ref.2

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 1 peptidase C16 domain.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host translation shutoff by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6W5-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6T6-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6T6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 175175Non-structural protein 1 Potential
PRO_0000291350
Chain176 – 772597Non-structural protein 2 Potential
PRO_0000291351
Chain773 – 26091837Non-structural protein 3 Potential
PRO_0000291352
Chain2610 – 3103494Non-structural protein 4 Potential
PRO_0000291353
Chain3104 – 34093063C-like proteinase Potential
PRO_0000291354
Chain3410 – 3699290Non-structural protein 6 Potential
PRO_0000291355
Chain3700 – 378283Non-structural protein 7 Potential
PRO_0000291356
Chain3783 – 3982200Non-structural protein 8 Potential
PRO_0000291357
Chain3983 – 4094112Non-structural protein 9 Potential
PRO_0000291358
Chain4095 – 4233139Non-structural protein 10 Potential
PRO_0000291359
Chain4234 – 5165932RNA-directed RNA polymerase Potential
PRO_0000291360
Chain5166 – 5766601Helicase Potential
PRO_0000291361
Chain5767 – 6296530Exoribonuclease Potential
PRO_0000291362
Chain6297 – 6633337Uridylate-specific endoribonuclease Potential
PRO_0000291363
Chain6634 – 6930297Putative 2'-O-methyl transferase Potential
PRO_0000291364

Regions

Transmembrane2015 – 203521Helical; Potential
Transmembrane2040 – 206021Helical; Potential
Transmembrane2081 – 210121Helical; Potential
Transmembrane2162 – 218221Helical; Potential
Transmembrane2183 – 220321Helical; Potential
Transmembrane2218 – 223821Helical; Potential
Transmembrane2621 – 264121Helical; Potential
Transmembrane2719 – 273921Helical; Potential
Transmembrane2865 – 288521Helical; Potential
Transmembrane2887 – 290721Helical; Potential
Transmembrane2916 – 293621Helical; Potential
Transmembrane2946 – 296621Helical; Potential
Transmembrane2970 – 299021Helical; Potential
Transmembrane3423 – 344321Helical; Potential
Transmembrane3449 – 346921Helical; Potential
Transmembrane3474 – 349421Helical; Potential
Transmembrane3517 – 353721Helical; Potential
Transmembrane3569 – 358921Helical; Potential
Transmembrane3592 – 361221Helical; Potential
Transmembrane3620 – 364021Helical; Potential
Domain930 – 1097168Macro
Domain1492 – 1757266Peptidase C16
Domain3104 – 3409306Peptidase C30
Domain4845 – 5007163RdRp catalytic
Domain5166 – 524984CV MBD
Domain5412 – 5603192(+)RNA virus helicase ATP-binding
Domain5604 – 5778175(+)RNA virus helicase C-terminal
Zinc finger1610 – 164738C4-type By similarity
Zinc finger4168 – 418417 By similarity
Zinc finger4211 – 422414 By similarity
Nucleotide binding5447 – 54548ATP Potential
Region2015 – 2238224HD1 By similarity
Region2621 – 2990370HD2 By similarity
Region3423 – 3640218HD3 By similarity
Compositional bias5170 – 519526Cys-rich
Compositional bias6832 – 68354Poly-Ser

Sites

Active site15331For PL-PRO activity By similarity
Active site16941For PL-PRO activity By similarity
Active site31441For 3CL-PRO activity By similarity
Active site32481For 3CL-PRO activity By similarity
Site175 – 1762Cleavage Potential
Site772 – 7732Cleavage; by PL-PRO Potential
Site2609 – 26102Cleavage; by PL-PRO Potential
Site3103 – 31042Cleavage; by 3CL-PRO Potential
Site3409 – 34102Cleavage; by 3CL-PRO Potential
Site3699 – 37002Cleavage; by 3CL-PRO Potential
Site3782 – 37832Cleavage; by 3CL-PRO Potential
Site3982 – 39832Cleavage; by 3CL-PRO Potential
Site4094 – 40952Cleavage; by 3CL-PRO Potential
Site4233 – 42342Cleavage; by 3CL-PRO Potential
Site5165 – 51662Cleavage; by 3CL-PRO Potential
Site5766 – 57672Cleavage; by 3CL-PRO Potential
Site6296 – 62972Cleavage; by 3CL-PRO Potential
Site6633 – 66342Cleavage; by 3CL-PRO Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 9ED06D0888C7EC7B

FASTA6,930769,729
        10         20         30         40         50         60 
MEGVPDPPKL KSMVVTTLKW CDPFANPNVT GWDIPIEEAL EYAKQQLRTP EPQLVFVPYY 

        70         80         90        100        110        120 
LSHAPGISGD RVVITDSIWY ATNFGWQPIR ELAMDKDGVR YGRGGTHGVL LPMQDPSFIM 

       130        140        150        160        170        180 
GDIDIQIRKY GIGANSPPDV LPLWDGFSDP GPDVGPYLDF PDNCCPTKPK AKRGGDVYLS 

       190        200        210        220        230        240 
DQYGFDNNGI LVEPVMKLLG VIKSDFTLEQ LLAALGKYRT EDGYDLPDGY VKVAIKVGRK 

       250        260        270        280        290        300 
AVPVLKQSIF TVVGVTEQLV PGYYYPFSTS SVVEHTKPTR GGPVGKTVEA VMLSLYGTNN 

       310        320        330        340        350        360 
YNPATPVARL KCSYCDYYGW TPLKDIGTVN CLCGAEFQLT SSCVDAESAG VIKPGCVMLL 

       370        380        390        400        410        420 
DKSPGMRLIP GNRTYVSFGG AIWSPIGKVN GVTVWVPRAY SIVAGEHSGA VGSGDTVAIN 

       430        440        450        460        470        480 
KELVEYLIEG IRVDADTLDN PTCATFIANL DCDTKAPVVH TVESLQGLCL ANKIMLGDKP 

       490        500        510        520        530        540 
LPTDEFHPFI VGLAYHVQRA CWYGALASRT FEAFRDFVRT EEERFAQFFG KVCAPINGCV 

       550        560        570        580        590        600 
YLAYTTGRVT LFSAYQVLNT AIAKSKDAFG GVAAIVVDML KPILEWVLKK MSIAKGAWLP 

       610        620        630        640        650        660 
YAEGLLALFK AQFTVVKGKF QFLRASLNSK CHSLCDLLTT IMSKLLTSVK WAGCKVDALY 

       670        680        690        700        710        720 
TGTYYYFSRK GVLTEVQLCA KRLGLLLTPK QQKMEVEVLD GDFDAPVTLT DLELEECTGV 

       730        740        750        760        770        780 
LEEVFGASDV KLVKGTLVSL ASKLFVRTED GFLYRYVKSG GVLGKAFRLR GGGVSKVTFG 

       790        800        810        820        830        840 
DEEVHTIPNT VTVNFSYDVC EGLDAILDKV MAPFQVEEGT KLEDLACVVQ KAVYERLSDL 

       850        860        870        880        890        900 
FSDCPAELRP INLEDFLTSE CFVYSKDYEK ILMPEMYFSL EDAVPVDDEM VDDIEDTVEQ 

       910        920        930        940        950        960 
ASDSDDQWLG DEGAEDCDNT IQDVDVATSM TTPCGYTKIA EHVYIKCADI VQEARNYSYA 

       970        980        990       1000       1010       1020 
VLVNAANVNL HHGGGVAGAL NRATNNAMQK ESSEYIKANG SLQPGGHVLL SSHGLASHGI 

      1030       1040       1050       1060       1070       1080 
LHVVGPDKRL GQDLALLDAV YAAYTGFDSV LTPLVSAGIF GFTVEESLCS LVKNVACTTY 

      1090       1100       1110       1120       1130       1140 
VVVYDRQLYE RALATSFDVP GPQSSVQHVP AIDWAEAVEV QESIVDQVET PSLGAVDTVD 

      1150       1160       1170       1180       1190       1200 
SNADSGLNET ARSPENVVGS VPDDVVADVE SCVRDLVRQV VKKVKRDKRP PPIVPQQTVE 

      1210       1220       1230       1240       1250       1260 
QQPQEISSPG DCNTVLVDVV SMSFSAMVNF GKEKGLLIPV VIDYPAFLKV LKRFSPKEGL 

      1270       1280       1290       1300       1310       1320 
FSSNGYEFYG YSRDKPLHEV SKDLNSLGRP LIMIPFGFIV NGQTLAVSAV SMRGLTVPHT 

      1330       1340       1350       1360       1370       1380 
VVVPSESSVP LYRAYFNGVF SGDTTAVQDF VVDILLNGAR DWDVLQTTCT VDRKVYKTIC 

      1390       1400       1410       1420       1430       1440 
KRGNTYLCFD DTNLYAITGD VVLKFATVSK ARAYLETKLC APEPLIKVLT TVDGINYSTV 

      1450       1460       1470       1480       1490       1500 
LVSTAQSYRA QIGTVFCDGH DWSNKNPMPT DEGTHLYKQD NFSSAEVTAI REYYGVDDSN 

      1510       1520       1530       1540       1550       1560 
IIARAMSIRK TVQTWPYTVV DGRVLLAQRD SNCYLNVAIS LLQDIDVSFS TPWVCRAYDA 

      1570       1580       1590       1600       1610       1620 
LKGGNPLPMA EVLIALGKAT PGVSDDAHMV LSAVLNHGTV TARRVMQTVC EHCGVSQMVF 

      1630       1640       1650       1660       1670       1680 
TGTDACTFYG SVVLDDLYAP VSVVCQCGRP AIRYVSEQKS PWLLMSCTPT QVPLDTSGIW 

      1690       1700       1710       1720       1730       1740 
KTAIVFRGPV TAGHYMYAVN GTLISVYDAN TRRRTSDLKL PATDILYGPT SFTSDSKVET 

      1750       1760       1770       1780       1790       1800 
YYLDGVKRTT IDPDFSKYVK RGDYYFTTAP IEVVAAPKLV TSYDGFYLSS CQNPQLAESF 

      1810       1820       1830       1840       1850       1860 
NKAINATKTG PMKLLTMYPN VAGDVVAISD DNVVAHPYGS LHMGKPVLFV TRPNTWKKLV 

      1870       1880       1890       1900       1910       1920 
PLLSTVVVNT PNTYDVLAVD PLPVNNETSE EPISVKAPIP LYGLKATMVL NGTTYVPGNK 

      1930       1940       1950       1960       1970       1980 
GHLLCLKEFT LTDLQTFYVE GVQPFVLLKA SHLSKVLGLR VSDSSLHVNH LSKGVVYAYA 

      1990       2000       2010       2020       2030       2040 
ATRLTTRVTT SLLGGLVTRS VRKTADFVRS TNPGSKCVGL LCLFYQLFMR FWLLVKKPPI 

      2050       2060       2070       2080       2090       2100 
VKVSGIIAYN TGCGVTTCVL NYLRSRCGNI SWSRLLKLLR YMLYIWFVWT CLTICGVWLS 

      2110       2120       2130       2140       2150       2160 
EPYAPSLVTR FKYFLGIVMP CDYVLVNETG TGWLHHLCMA GMDSLDYPAL RMQQHRYGSP 

      2170       2180       2190       2200       2210       2220 
YNYTYILMLL EAFFAYLLYT PALPIVGILA VLHLIVLYLP IPLGNSWLVV FLYYIIRLVP 

      2230       2240       2250       2260       2270       2280 
FTSMLRMYIV IAFLWLCYKG FLHVRYGCNN VACLMCYKKN VAKRIECSTV VNGVKRMFYV 

      2290       2300       2310       2320       2330       2340 
NANGGTHFCT KHNWNCVSCD TYTVDSTFIC RQVALDLSAQ FKRPIIHTDE AYYEVTSVEV 

      2350       2360       2370       2380       2390       2400 
RNGYVYCYFE SDGQRSYERF PMDAFTNVSK LHYSELKGAA PAFNVLVFDA TNRIEENAVK 

      2410       2420       2430       2440       2450       2460 
TAAIYYAQLA CKPILLVDKR MVGVVGDDAT IARAMFEAYA QNYLLKYSIA MDKVKHLYST 

      2470       2480       2490       2500       2510       2520 
ALQQISSGMT VESVLKVFVG STRAEAKDLE SDVDTNDLVS CIRLCHQEGW EWTTDSWNNL 

      2530       2540       2550       2560       2570       2580 
VPTYIKQDTL STLEVGQFMT ANAKYVNANI AKGAAVNLIW RYADFIKLSE SMRRQLKVAA 

      2590       2600       2610       2620       2630       2640 
RKTGLNLLVT TSSLKADVPC MVTPFKIIGG HRRIVSWRRV LIHVFMLLVV LNPQWFTPWY 

      2650       2660       2670       2680       2690       2700 
IMRPIEYNVV DFKVIDNAVI RDITSADQCF ANKFSAFENW YSNRYGSYVN SRGCPMVVGV 

      2710       2720       2730       2740       2750       2760 
VSDIVGSLVP GLPARFLRVG TTLLPLVNYG LGAVGSVCYT PHYAINYDVF DTSACVLAAT 

      2770       2780       2790       2800       2810       2820 
CTLFSSASGE RMPYCADAAL IQNASRYDML KPHVMYPFYE HSGYIRFPEV ISAGVHIVRT 

      2830       2840       2850       2860       2870       2880 
MAMEYCKVGR CDVSEAGLCM SLQPRWVVNN AYFRQQSGVY CGTSAFDLFM NMLLPIFTPV 

      2890       2900       2910       2920       2930       2940 
GAVDITTSIL MGALLAVVVS MSLYYLLRFR RAFGDYSGVI FTNILAFVLN VIVLCLEGPY 

      2950       2960       2970       2980       2990       3000 
PMLPSIYAMV FLYATCYFGS DIACMMHVSF LIMFAGVVPL WVTVLYIVVV LSRHILWFAS 

      3010       3020       3030       3040       3050       3060 
LCTKRTVQVG DLAFHSFQDA ALQTFMLDKE VFLRLKREIS SDAYFKYLAM YNKYKYYSGP 

      3070       3080       3090       3100       3110       3120 
MDTAAYREAA CSHLVMALEK YSNGGGDTIY QPPRCSVASA ALQAGLTRMA HPSGLVEPCL 

      3130       3140       3150       3160       3170       3180 
VKVNYGSMTL NGIWLDNFVI CPRHVMCSRD ELANPDYPRL SMRAANYDFH VSQNGHNIRV 

      3190       3200       3210       3220       3230       3240 
IGHTMEGSLL KLTVDVNNPK TPAYSFIRVS TGQAMSLLAC YDGLPTGVYT CTLRSNGTMR 

      3250       3260       3270       3280       3290       3300 
ASFLCGSCGS PGFVMNGKEV QFCYLHQLEL PNGTHTGTDF SGVFYGPFED KQVPQLAAPD 

      3310       3320       3330       3340       3350       3360 
CTITVNVLAW LYAAVLSGEN WFLTKSSISP AEFNNCAVKY MCQSVTSESL QVLQPLAAKT 

      3370       3380       3390       3400       3410       3420 
GISVERMLSA LKVLLSAGFC GRTIMGSCSL EDEHTPYDIG RQMLGVKLQG KFQSMFRWTL 

      3430       3440       3450       3460       3470       3480 
QWFAIIFVLT ILILLQLAQW TFVGALPFTL LLPLIGFVAV CVGFVSLLIK HKHTYLTVYL 

      3490       3500       3510       3520       3530       3540 
LPVAMVTAYY NFQYTPEGVQ GYLLSLYNYV NPGRIDVIGT DLLTMLIISV ACTLLSVRMV 

      3550       3560       3570       3580       3590       3600 
RTDAYSRIWY VCTAVGWLYN CWTGSADTVA ISYLTFMVSV FTNYTGVACA SLYAAQFMVW 

      3610       3620       3630       3640       3650       3660 
VLKFLDPTIL LLYGRFRCVL VCYLLVGYLC TCYFGVFNLI NRLFRCTLGN YEYVVSSQEL 

      3670       3680       3690       3700       3710       3720 
RYMNSHGLLP PTNSWQALML NIKLAGIGGI PIYRVSTIQS NMTDLKCTSV VLLSVLQQLR 

      3730       3740       3750       3760       3770       3780 
VESSSKLWAL CVKLHNEILA SNSPTEAFEA FVSLLSVLLS LPGAINLDEL CSSILENNSV 

      3790       3800       3810       3820       3830       3840 
LQAVASEFSN LSSYVDYENA QKAYDTAVAT GAPASTVNAL KKAMNVAKSV LDKDVATTRK 

      3850       3860       3870       3880       3890       3900 
LERMSELAMT AMYKQARAED RRSKVTAAMQ TMLFNMIRRL DSDALSNILN NARNGVVPLG 

      3910       3920       3930       3940       3950       3960 
VIPRTAANKL LLVVPDFSVY TATITMPTLT YAGSAWDVMQ VADADGKTVN ATDITRENSV 

      3970       3980       3990       4000       4010       4020 
NLAWPLVVTA QRQQATSPVK LQNNELMPQT VKRMNVVAGV SQTACVTDAV AYYNATKEGR 

      4030       4040       4050       4060       4070       4080 
HVMAILADTD GLAFAKVEKS TGDGFVILEL EPPCKFMVDT PKGPALKYLY FTKGLKNLCR 

      4090       4100       4110       4120       4130       4140 
GTVLGTLACT VRLHAGSATE VASNSSILSL CSFSVDPEAT YKDYLDNGGS PIGNCVKMLT 

      4150       4160       4170       4180       4190       4200 
PHTGTGLAIT AKPDANIDQE SFGGASCCLY CRCHIEHPGA SGVCKYKGKF VQIPLVGVND 

      4210       4220       4230       4240       4250       4260 
PIGFCIRNVV CAVCNMWQGY GCPCSSLREI NLQARDECFL NRVRGTSGVA RLVPLGSGVQ 

      4270       4280       4290       4300       4310       4320 
PDIVLRAFDI CNTKVAGFGL HLKNNCCRYQ ELDADGTQLD SYFVVKRHTE SNYLLEQRCY 

      4330       4340       4350       4360       4370       4380 
EKLKDCGVVA RHDFFKFNIE GVMTPHVSRE RLTKYTMADL VYSLRHFDNN NCDTLKEILV 

      4390       4400       4410       4420       4430       4440 
LRGCCTADYF DRKDWYDPVE NPDIIRVYHN LGETVRKAVL SAVKMADSMV EQGLIGVLTL 

      4450       4460       4470       4480       4490       4500 
DNQDLNGQWY DFGDFIEGPA GAGVAVMDTY YSLAMPVYTM TNMLAAECHV DGDFSKPKRV 

      4510       4520       4530       4540       4550       4560 
WDICKYDYTQ FKYSLFSKYF KYWDMQYHPN CVACADDRCI LHCANFNILF SMVLPNTSFG 

      4570       4580       4590       4600       4610       4620 
PLVQKIYVDG VPFVVSTGYH YRELGVVMNQ DIRQHAQRLS LRELLVYAAD PAMHVAASNA 

      4630       4640       4650       4660       4670       4680 
LADKRTVCMS VAAMTTGVTF QTVKPGQFNE DFYNFAVKCG FFKEGSTISF KHFFYAQDGN 

      4690       4700       4710       4720       4730       4740 
AAISDYDYYR YNLPTMCDIK QLLFSLEVVD KYFDCYDGGC LQASQVVVAN YDKSAGFPFN 

      4750       4760       4770       4780       4790       4800 
KFGKARLYYE SLSYADQDEL FAYTKRNVLP TITQMNLKYA ISAKNRARTV AGVSIASTMT 

      4810       4820       4830       4840       4850       4860 
NRQFHQKMLK SIAAARGASV VIGTTKFYGG WNRMLRTLCE GVENPHLMGW DYPKCDRAMP 

      4870       4880       4890       4900       4910       4920 
NLLRIFASLI LARKHATCCN ASERFYRLAN ECAQVLSEMV LCGGGFYVKP GGTSSGDSTT 

      4930       4940       4950       4960       4970       4980 
AYANSVFNIC QAVSANLNTF LSIDGNKIYT TYVQELQRRL YLGIYRSNTV DNELVLDYYN 

      4990       5000       5010       5020       5030       5040 
YLRKHFSMMI LSDDGVVCYN ADYAQKGYVA DIQGFKELLY FQNNVFMSES KCWVEPDITK 

      5050       5060       5070       5080       5090       5100 
GPHEFCSQHT MLVDMKGEQV YLPYPDPSRI LGAGCFVDDL LKTDGTLMME RYVSLAIDAY 

      5110       5120       5130       5140       5150       5160 
PLTKHPDPEY QNVFWCYLQY IKKLHEELTG HLLDTYSVML ASDNASKYWE VEFYENMYME 

      5170       5180       5190       5200       5210       5220 
SATLQSVGTC VVCNSQTSLR CGGCIRRPFL CCKCCYDHVV STTHKLVLSV TPYVCNNPSC 

      5230       5240       5250       5260       5270       5280 
DVADVTQLYL GGMSYYCRDH RPPISFPLCA NGQVFGLYKN ICTGSPDVAD FNSLATCDWS 

      5290       5300       5310       5320       5330       5340 
NSKDYVLANT ATERLKLFAA ETLRATEENA KQAYASAVVK EVLSDRELVL SWETGKTRPP 

      5350       5360       5370       5380       5390       5400 
LNRNYVFTGF HITKNSKVQL GEYIFEKGDY GDVVNYRSST TYKLQVGDYF VLTSHSVQPL 

      5410       5420       5430       5440       5450       5460 
SSPTLLPQER YTKLVGLYPA MNVPESFASN VVHYQRVGMS RYTTVQGPPG TGKSHLSIGL 

      5470       5480       5490       5500       5510       5520 
ALYYPSAKIV YTACSHAAVD ALCEKAHKNL PINRCSRIVP AKARVECFSK FKVNDVGAQY 

      5530       5540       5550       5560       5570       5580 
VFSTINALPE TTADILVVDE VSMCTNYDLS MINARVRAKH IVYVGDPAQL PAPRTLLTKG 

      5590       5600       5610       5620       5630       5640 
TLAPEHFNSV CRLMVAVGPD IFLATCYRCP KEIVDTVSAL VYDKKLKANK VTTGECYKCY 

      5650       5660       5670       5680       5690       5700 
YKGSVTHDSS SAINKPQLGL VKEFLIKNPK WQSAVFISPY NSQNSVARRM LGLQTQTVDS 

      5710       5720       5730       5740       5750       5760 
SQGSEFDYVI YCQTSDTAHA LNVNRFNVAI TRAKKGILCV MSDSTLYESL EFTPLDVNDY 

      5770       5780       5790       5800       5810       5820 
VKPKMQSEVT VGLFKDCAKA EPLGPAYAPT FVSVNDKFKL NESLCVHFDT TELQMPYNRL 

      5830       5840       5850       5860       5870       5880 
ISKMGFKFDL NIPGYSKLFI TREQAIREVR GWVGFDVEGA HACGPNIGTN LPLQIGFSTG 

      5890       5900       5910       5920       5930       5940 
VNFVVTPSGY IDTESGSRLA NVVSKAPPGD QFKHLIPLMR KGEPWSVVRK RIVEMLCDTL 

      5950       5960       5970       5980       5990       6000 
DGVSDTVTFV TWAHGFELTT LHYFAKVGPE RKCFMCPRRA TLFSSVYGAY SCWSHHRHIG 

      6010       6020       6030       6040       6050       6060 
GADFVYNPFL VDVQQWGYVG NLQVNHDNVC DVHKGAHVAS CDAIMTRCLA IHDCFCGEVN 

      6070       6080       6090       6100       6110       6120 
WDVEYPIIAN ELAINRACRS VQRVVLKAAV KALHIETIYD IGNPKAIKVY GVNVNNWNFY 

      6130       6140       6150       6160       6170       6180 
DTNPVVEGVK QLHYVYDVHR DQFKDGLAMF WNCNVDCYPH NALVCRFDTR VLSKLNLAGC 

      6190       6200       6210       6220       6230       6240 
NGGSLYVNQH AFHTDAFNKN AFVNLKPLPF FYYSDTACEN ATGVSTNYVS EVDYVPLKSN 

      6250       6260       6270       6280       6290       6300 
VCITRCNLGG AVCKKHADEY RNFLESYNTM VSAGFTLWVD KTFDVFNLWS TFVKLQSLEN 

      6310       6320       6330       6340       6350       6360 
VAYNVLKSGH FTAVAGELPV AILNDRLYIK EDGADKLLFT NNTCLPTNVA FELWAKRSVN 

      6370       6380       6390       6400       6410       6420 
VVPEVKLLRN LGVTCTYNLV IWDYESNAPL VPNTVGICTY TDLTKLDDQV VLVDGRQLDA 

      6430       6440       6450       6460       6470       6480 
YSKFCQLKNA IYFSPSKPKC VCTRGPTHAS INGVVVEAPD RGTAFWYAMR KDGAFVQPTD 

      6490       6500       6510       6520       6530       6540 
GYFTQSRTVD DFQPRTQLEI DFLDLEQSCF LDKYDLHDLG LEHIVYGQFD GTIGGLHLLI 

      6550       6560       6570       6580       6590       6600 
GAVRRKRTAH LVMETVLGTD TVTSYAVIDQ PTASSKQVCS VVDIILDDFI ALIKAQDRSV 

      6610       6620       6630       6640       6650       6660 
VSKVVQCCLD FKVFRFMLWC KGGKISTFYP QLQAKQDWKP GYSMPALYKV QNAVLEPCLL 

      6670       6680       6690       6700       6710       6720 
HNYGQAARLP SGTLMNVAKY TQLCQYLNTC SLAVPAKMRV MHFGAGSDKG VCPGTAVLKQ 

      6730       6740       6750       6760       6770       6780 
WLPADAYLVD NDLCYCASDA DSTYVGSCET FFSVNKWDFI FSDMYDARTK NTSGDNTSKE 

      6790       6800       6810       6820       6830       6840 
GFFTYLTGFI RSKLALGGSI AIKITEHSWS ADLYAIMGHF NWWTCFCTSV NSSSSEAFLI 

      6850       6860       6870       6880       6890       6900 
GVNYIGVGAL LDGWQMHANY VFWRNSTVMQ LSSYSLYDLQ RFPLRLKGTP VMSLKEDQLN 

      6910       6920       6930 
ELVLNLIRAG RLIVRDAVDI GVRGVACSGV 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6T6.

References

[1]"Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features."
Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y.
J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HKU9-1.
[2]"Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses."
Tohya Y., Narayanan K., Kamitani W., Huang C., Lokugamage K., Makino S.
J. Virol. 83:5282-5288(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF NSP1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF065513 Genomic RNA. Translation: ABN10910.1.
RefSeqYP_001039970.1. NC_009021.1.

3D structure databases

ProteinModelPortalP0C6W5.
SMRP0C6W5. Positions 934-1096, 3106-3403, 3699-3782, 3820-3972, 3985-4094, 4104-4224.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0C6W5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4836014.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_BCHK9
AccessionPrimary (citable) accession number: P0C6W5
Secondary accession number(s): A3EXG5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries