Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0C6W4 (R1AB_BCHK5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    Leader protein
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65 homolog
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name=PL-PRO
  4. Non-structural protein 4
    Short name=nsp4
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    nsp5
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
  8. Non-structural protein 8
    Short name=nsp8
  9. Non-structural protein 9
    Short name=nsp9
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismBat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004) [Complete proteome]
Taxonomic identifier694008 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostPipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus) [TaxID: 105295]

Protein attributes

Sequence length7182 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 1 peptidase C16 domain.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host translation shutoff by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6W4-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6T5-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6T5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Non-structural protein 1 Potential
PRO_0000290303
Chain196 – 851656Non-structural protein 2 Potential
PRO_0000290304
Chain852 – 28301979Non-structural protein 3 Potential
PRO_0000290305
Chain2831 – 3338508Non-structural protein 4 Potential
PRO_0000290306
Chain3339 – 36443063C-like proteinase Potential
PRO_0000290307
Chain3645 – 3936292Non-structural protein 6 Potential
PRO_0000290308
Chain3937 – 401983Non-structural protein 7 Potential
PRO_0000290309
Chain4020 – 4218199Non-structural protein 8 Potential
PRO_0000290310
Chain4219 – 4328110Non-structural protein 9 Potential
PRO_0000290311
Chain4329 – 4467139Non-structural protein 10 Potential
PRO_0000290312
Chain4468 – 5401934RNA-directed RNA polymerase Potential
PRO_0000290313
Chain5402 – 5999598Helicase Potential
PRO_0000290314
Chain6000 – 6523524Exoribonuclease Potential
PRO_0000290315
Chain6524 – 6874351Uridylate-specific endoribonuclease Potential
PRO_0000290316
Chain6875 – 7182308Putative 2'-O-methyl transferase Potential
PRO_0000290317

Regions

Transmembrane2158 – 217821Helical; Potential
Transmembrane2196 – 221621Helical; Potential
Transmembrane2268 – 228821Helical; Potential
Transmembrane2372 – 239221Helical; Potential
Transmembrane2396 – 241621Helical; Potential
Transmembrane2421 – 244121Helical; Potential
Transmembrane2848 – 286821Helical; Potential
Transmembrane3119 – 313921Helical; Potential
Transmembrane3152 – 317221Helical; Potential
Transmembrane3203 – 322321Helical; Potential
Transmembrane3650 – 367021Helical; Potential
Transmembrane3684 – 370421Helical; Potential
Transmembrane3709 – 372921Helical; Potential
Transmembrane3760 – 377718Helical; Potential
Transmembrane3782 – 380221Helical; Potential
Transmembrane3823 – 384321Helical; Potential
Transmembrane3855 – 387521Helical; Potential
Domain1186 – 1345160Macro
Domain1628 – 1902275Peptidase C16
Domain3339 – 3644306Peptidase C30
Domain5081 – 5243163RdRp catalytic
Domain5402 – 548584CV MBD
Domain5658 – 5839182(+)RNA virus helicase ATP-binding
Domain5840 – 6014175(+)RNA virus helicase C-terminal
Zinc finger1748 – 178538C4-type By similarity
Zinc finger4402 – 441817 By similarity
Zinc finger4444 – 445714 By similarity
Nucleotide binding5683 – 56908ATP By similarity
Region2158 – 2441284HD1 By similarity
Region2848 – 3223376HD2 By similarity
Region3650 – 3875226HD3 By similarity
Compositional bias964 – 1066103Glu-rich
Compositional bias5406 – 543126Cys-rich

Sites

Active site16681For PL-PRO activity By similarity
Active site18381For PL-PRO activity By similarity
Active site33791For 3CL-PRO activity By similarity
Active site34861For 3CL-PRO activity By similarity
Site195 – 1962Cleavage Potential
Site851 – 8522Cleavage; by PL-PRO Potential
Site2830 – 28312Cleavage; by PL-PRO Potential
Site3338 – 33392Cleavage; by 3CL-PRO Potential
Site3644 – 36452Cleavage; by 3CL-PRO Potential
Site3936 – 39372Cleavage; by 3CL-PRO Potential
Site4019 – 40202Cleavage; by 3CL-PRO Potential
Site4218 – 42192Cleavage; by 3CL-PRO Potential
Site4328 – 43292Cleavage; by 3CL-PRO Potential
Site4467 – 44682Cleavage; by 3CL-PRO Potential
Site5401 – 54022Cleavage; by 3CL-PRO Potential
Site5999 – 60002Cleavage; by 3CL-PRO Potential
Site6523 – 65242Cleavage; by 3CL-PRO Potential
Site6874 – 68752Cleavage; by 3CL-PRO Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 455E36D8EF6FD1E1

FASTA7,182799,932
        10         20         30         40         50         60 
MSFVAGVAPQ GARGKYRAEL NTEKRTDHVS LKASLCDAGD LVLKISPWFM DGESAYKHVS 

        70         80         90        100        110        120 
EQLSKGSKLL FVPQTLKGFI RHLPGPRVYL VERLTGGTYS DPFMVNQLAY QNAAGEGVIG 

       130        140        150        160        170        180 
TTLQGKRVGM FFPFDADLVT GEFQFLLRKK GFGGNRFRDA PWDYNWTPYS DLMDALEADP 

       190        200        210        220        230        240 
CGKYSQSLLK KLVGGDFTPI DQYMCGKNGK PIAEFAALMA SEGITKLADV EAEVKSRTDS 

       250        260        270        280        290        300 
DRYIVFKNKL YRIVWNVQRK DVAYSKQSAF TMNSIVQLDT MEDVPRHSFT IGSEIQVIAP 

       310        320        330        340        350        360 
STAVQANGHL NLKQRLLYAF YGKQAVSEPN YIYHSAYVDC TSCGKGSWLT GNAVQGFACD 

       370        380        390        400        410        420 
CGAHYCANDV DLQSSGLVRK NAVLLTTCPC NKDGECKHTL PQLVSMMTDK CDVEVVGKTF 

       430        440        450        460        470        480 
ILTYGGVIYA YMGCSGGTMH FIPRAKSCVS KIGDAIFTGC TGTWSKVCET ANLFLERAQH 

       490        500        510        520        530        540 
AINFVNEFVL TETVVALLSG TTSSIEELRD LCRNATFEKV RDYLTPRGWI VTMGSYIEGV 

       550        560        570        580        590        600 
INVGAAGVCN AALNAPFIVL SGLGESFKKV AATPWKLCSS LRETLDHYAD SITYRVFPYD 

       610        620        630        640        650        660 
IPCDVTDYTA LLLDCAVLTG ASAYFVARYV DEKVEQLTNL VFSSCQSAVA AFVQACMSTY 

       670        680        690        700        710        720 
KATAKFISDM FTLIKVVSER LYVYTSVGFV VVGDYSSQLL KQFMHILSKA MQLLHTTVSW 

       730        740        750        760        770        780 
AGSKLPSVVY NGRDSLVFPS GTYYCVSTQG RSLQDQFDLV IPGDLSKKQI GILEPTPNST 

       790        800        810        820        830        840 
TVDKKINTNV VEVVVGQLEP TKEHSPELVV GDYVIISNKI FVRSVEDSET VFYPLCTDGK 

       850        860        870        880        890        900 
IVPTLFRLKG GAPPKGVKFG GEQTKEITAV RSVSVDYDVH PVLDALLAGS ELATFTVEKD 

       910        920        930        940        950        960 
LPVKDFVDVV KDEVIELLSK LLRGYNVDGF DLEDFADTPC YVYNAEGDLA WSSTMTFSVN 

       970        980        990       1000       1010       1020 
PVEEVEEECD DDYVEDEYLS EEMLVEEDEN SWAAAVEAVI PMEDVQLDTL VAEIDVSEPA 

      1030       1040       1050       1060       1070       1080 
DDVAEQASTE EVEVPSACVL EASQVANAAE VESCEAEVSS SIPLHEDANA AKANDCAEGM 

      1090       1100       1110       1120       1130       1140 
PALDSTETVS KLSVDTPVGD VTQDDATSSN ATVISEDVHT ATHSKGLVAV PEVVPEKALG 

      1150       1160       1170       1180       1190       1200 
TSVERMRSTS EWTVVETSLK QETAVIVKND SSAKPQRVKK PKAENPLKNF KHIVLNNDVT 

      1210       1220       1230       1240       1250       1260 
LVFGDAIAVA RATEDCILVN AANTHLKHGG GIAAAIDRAS GGLVQAESDD YVNFYGPLNV 

      1270       1280       1290       1300       1310       1320 
GDSTLLKGHG LATGILHVVG PDARANQDIQ LLKRCYKAFN KYPLVVSPLI SAGIFCVEPR 

      1330       1340       1350       1360       1370       1380 
VSLEYLLSVV HTKTYVVVNS EKVYNDLAAP KPPTGLTYSH EGWRGIIRNA KSFGFTCFIC 

      1390       1400       1410       1420       1430       1440 
TDQSANAKLL KGRGVDLTKK TQTVDGVKYY LYSSKDPLTD IITAANACKG ICAMPIGYVT 

      1450       1460       1470       1480       1490       1500 
HGLDLAQAGQ QVKKITVPYV CLLASKDQVP ILNSDVAVQT PEQSFINTVI ANGGYHCWHL 

      1510       1520       1530       1540       1550       1560 
VTGELIVKGV SYRKLLNWSD QTICYADNKF YVVKGQIALP FDSLEKCRTY LTSRAAQQKN 

      1570       1580       1590       1600       1610       1620 
VDVLVTIDGV NFRTVVLNNT TTYRVQLGSV FYKGSDISDT IPTEKMSGEA VYLADNLSEA 

      1630       1640       1650       1660       1670       1680 
EKAVLSEVYG TADTAFLHRY YSLLALVKKW KYTVHDGVKS LKLNSNNCYV NVTMLMLDML 

      1690       1700       1710       1720       1730       1740 
KEIKFIVPAL QAAYLKHKGG DSTEFIALIM AYGDCTYGEP DDASRLLHTI LSKAELTTQA 

      1750       1760       1770       1780       1790       1800 
KMVWRQWCNV CGVQDTTTTG LKACIYVGMN SLDELHATHE ECCQCGDVRK RQLVEHNAPW 

      1810       1820       1830       1840       1850       1860 
LLLSGLNEAK VMTPTSQSAG PDYTAFNVFQ GVETSVGHYL HVRVKDNLLY KYDSGSLSKT 

      1870       1880       1890       1900       1910       1920 
SDMKCKMTDV YYPKQRYSAD CNVVVYSLDG NTWADVDPDL SAFYMKDGKY FTKKPVIEYS 

      1930       1940       1950       1960       1970       1980 
PATILSGSVY TNSCLVGHDG TIGSDAISSS FNNLLGFDNS KPVSKKLTYS FFPDFEGDVI 

      1990       2000       2010       2020       2030       2040 
LTEYSTYDPI YKNGAMLHGK PILWVNNSKF DSALNKFNRA TLRQVYDIAP VTLENKYTVL 

      2050       2060       2070       2080       2090       2100 
QDNQIQQVEV EAPKEDAKPQ SPVQVAEDID NKLPIIKCKG LKKPFVKDGY SFVNDPQGVN 

      2110       2120       2130       2140       2150       2160 
VIDTLGIDDL RALYVDRNLR LIVLKENNWS ALFNIHTVEK GDLSVIAASG SITRRVKILL 

      2170       2180       2190       2200       2210       2220 
GASSLFAQFA SVTVNVTTAM GKALGRMTRN VITNTGIIGQ GFALLKMLLI LPFTFWKSKN 

      2230       2240       2250       2260       2270       2280 
QSTVKVEVGA LRTAGIVTTN VVKQCASAAY DVLVVKFKRI DWKSTLRLLF LICTTGLLLS 

      2290       2300       2310       2320       2330       2340 
SLYYLFLFHQ VLTSDVMLDG AEGMLATYRE LRSYLGIHSL CDGMVEAYRN VSYDVNDFCS 

      2350       2360       2370       2380       2390       2400 
NRSALCNWCL IGQDSLTRYS AFQMIQTHVT SYVINIDWVW FVMEFALAYV LYTSTFNVLL 

      2410       2420       2430       2440       2450       2460 
LVVSSQYFFS YTGAFVNWRS YNYLVSGYFF CVTHIPLLGL VRIYNFLACL WFLRRFYNHV 

      2470       2480       2490       2500       2510       2520 
INGCKDTACL LCYKRNRLTR VEASTVVCGS KRTFYIVANG GTSFCCRHNW NCVDCDTAGI 

      2530       2540       2550       2560       2570       2580 
GNTFICEEVA NDLTTSLRRL VKPTDKSHYY VESVTVKDSV VQLHYSREGA SCYERYPLCY 

      2590       2600       2610       2620       2630       2640 
FTNLDKLKFK EVCKTPTGIP EHNFLIYDSS DRGQENLARS ACVYYSQVLS KPMLLVDSNM 

      2650       2660       2670       2680       2690       2700 
VTTVGDSREI ASKMLDSYVN SFISLFGVNR DKLDKLVATA RDCVKRGDDF QTVIKTFTDA 

      2710       2720       2730       2740       2750       2760 
ARGPAGVESD VETSSIVDAL QYAYKHDLQL TTEGFNNYVP SYIKPDSVAT ADLGCLIDLN 

      2770       2780       2790       2800       2810       2820 
AASVNQTSIR NANGACIWNS SDYMKLSDSL KRQIRIACRK CNIPFRLTTS RLRSADNILS 

      2830       2840       2850       2860       2870       2880 
VKFSATKLSG GAPKWLLKLR DFTWKSYCVV TLVVFAMAVL SYLCLPAFNM SQVSFHEDRI 

      2890       2900       2910       2920       2930       2940 
LTYKVVENGI IRDITPSDTC FANKYQSFSK WFNEHYGGLF NNDISCPVTV AVIAGVAGAR 

      2950       2960       2970       2980       2990       3000 
VPNLPANVAW VGRQIVLFVS RVFASSNNVC YTPTAEIPYE RFSDSGCVLA SECTLFRDAE 

      3010       3020       3030       3040       3050       3060 
GKINPYCYDP TVLPGASAYD QMKPHVRYDM YDSDMYIKFP EVVFESTLRI TKTLATRYCR 

      3070       3080       3090       3100       3110       3120 
FGSCEDANEG VCITTNGSWA IYNDHYANKP GVYCGDNYFD IVRRLGLSLF QPVTYFQLST 

      3130       3140       3150       3160       3170       3180 
SLALGVMLCI FLTIAFYYVN KVKRALADYT QCAVVAVAAA LLNSLCLCFV VSNPLLVLPY 

      3190       3200       3210       3220       3230       3240 
TALYYYATFY LTGEPAFVMH VSWFVMFGTV VPIWMVFAYI VGVCLRHLLW VMAYFSKKHV 

      3250       3260       3270       3280       3290       3300 
EVFTDGKLNC SFQDAAANIF VINKDTYVAL RNSITQDSYN RYLSMFNKYK YYSGAMDTAS 

      3310       3320       3330       3340       3350       3360 
YREASAAHLC KALQVYSETG SDVLFQPPNC SVTSSVLQSG LVKMAAPSGV VENCMVQVTC 

      3370       3380       3390       3400       3410       3420 
GSMTLNGLWL DNYVWCPRHV MCPADQLSDP NYDALLVSKT NLSFIVQKNV GAPANLRVVG 

      3430       3440       3450       3460       3470       3480 
HTMVGTLLKL TVESANPQTP AYTFTTVKPG ASFSVLACYN GRPTGVFMVN MRQNSTIKGS 

      3490       3500       3510       3520       3530       3540 
FLCGSCGSVG YTQEGNVINF CYMHQMELSN GTHTGCAFDG VMYGAFEDRQ VHQVQLSDKY 

      3550       3560       3570       3580       3590       3600 
CTINIVAWLY AAILNGCNWF VKPNKTGIAT FNEWAMSNQF TEFIGTQSVD MLAHKTGVSV 

      3610       3620       3630       3640       3650       3660 
EQLLYAIQTL HKGFQGKTIL GNSMLEDEFT PDDVNMQVMG VVMQSGVKRI SYGLVHWLFT 

      3670       3680       3690       3700       3710       3720 
TLLLAYVATL QLTKFTIWNY LFEVIPLQLT PLVLCVMACV MLTVKHKHTF LTLFLLPTAI 

      3730       3740       3750       3760       3770       3780 
CLTYANIVYE PQTPVSSALI AVANWLNPAS VYMRTTHTDL GVYLSLCFAL AVVVRRLYRP 

      3790       3800       3810       3820       3830       3840 
NASNLALALG SAMVWFYTYT TGDCSSPLTY LMFLTTLTSD YTVTVFLAVN VAKFFARVVF 

      3850       3860       3870       3880       3890       3900 
LYAPHAGFIF PEVKLVLLMY LAVGYFCTVY FGVFSLLNLK LRVPLGVYDY TVSTQEFRYL 

      3910       3920       3930       3940       3950       3960 
TGNGLHAPRN SWEALRLNMK LIGIGGTPCI KIASVQSKLT DLKCTSVVLL SVLQQLHLEA 

      3970       3980       3990       4000       4010       4020 
NSKAWAHCVK LHNDILAATD PTEAFDNFVC LFATLMSFSA NVDLEALASD LLDHPSVLQA 

      4030       4040       4050       4060       4070       4080 
TLSEFSHLAS YAELEAAQSS YQKALNSGDA SPQVLKALQK AVNIAKNAYE KDKAVARKLE 

      4090       4100       4110       4120       4130       4140 
RMAEQAMTSM YKQARAEDKK AKIVSAMQTM LFGMIKKLDN DVLNGVISNA RNGCVPLSVV 

      4150       4160       4170       4180       4190       4200 
PLCASNKLRV VIPDITIWNK VVTWPSLSYA GALWDISLIN NVDGEVVKSS DVTETNESLT 

      4210       4220       4230       4240       4250       4260 
WPLVLECTRA ASSAVTLQNN EIRPSGLKTM VVSAGIDHAN CNTSSLAYYE PVEGRKMLMG 

      4270       4280       4290       4300       4310       4320 
ILSENAHLKW AKVEGRDGFV NIELQPPCKF LIAGPKGPEV RYLYFVKNLN NLHRGQLLGH 

      4330       4340       4350       4360       4370       4380 
IAATVRLQAG SNTEFAINSS VLSAVTFSVD PGKAYLDFVN AGGAPLTNCV KMLTPKTGTG 

      4390       4400       4410       4420       4430       4440 
IAVSVKPEAN ADQDTYGGAS VCLYCRAHIE HPDVTGVCKF KGKFVQVPLH IRDPVGFCLQ 

      4450       4460       4470       4480       4490       4500 
NTPCNVCQFW IGHGCNCDAL RGTTIPQSKD SNFLNRVRGS IVNARIEPCA SGLTTDVVFR 

      4510       4520       4530       4540       4550       4560 
AFDICNYKAK VAGIGKYYKT NTCRFVEVDD EGHRLDSFFV VKRHTMENYE LEKRCYDLVK 

      4570       4580       4590       4600       4610       4620 
DCDAVAVHDF FIFDVDKVKT PHIVRQRLTE YTMMDLVYAL RHFDQNNCEV LKSILVKYGC 

      4630       4640       4650       4660       4670       4680 
CDASYFDNKL WFDFVENPNV ISVYHKLGER IRQAVLNTVK FCDQMVKSGL VGVLTLDNQD 

      4690       4700       4710       4720       4730       4740 
LNGKWYDFGD FVITQPGAGV AIVDSYYSYL MPVLSMTNCL AAETHRDCDL TKPLIEWPLL 

      4750       4760       4770       4780       4790       4800 
EYDYTDYKIG LFEKYFKXWD QQYHPNCVNC TDDRCVLHCA NFNVLFSMTL PGTSFGPIVR 

      4810       4820       4830       4840       4850       4860 
KIFVDGVPFV ISCGYHYKEL GLVMNMDVSL HRHRLSLKEL MMYAADPAMH IASASALWDL 

      4870       4880       4890       4900       4910       4920 
RTPCFSVAAL TTGLTFQTVR PGNFNKDFYD FVVSKGFFKE GSSVTLRHFF FAQDGHAAIT 

      4930       4940       4950       4960       4970       4980 
DYSYYAYNLP TMCDIKQMLF CMEVVDRYFE IYDGGCLNAS EVIVNNLDKS AGHPFNKFGK 

      4990       5000       5010       5020       5030       5040 
ARVYYESLSY QEQDELFAMT KRNVLPTITQ MNLKYAISAK NRARTVAGVS ILSTMTNRQY 

      5050       5060       5070       5080       5090       5100 
HQKMLKSMAA TRGSTCVIGT TKFYGGWDFM LKTLYKDVDN PHLMGWDYPK CDRAMPNMCR 

      5110       5120       5130       5140       5150       5160 
IFASLILARK HSTCCTNTDR FYRLANECAQ VLSEYVLCGG GYYVKPGGTS SGDATTAYAN 

      5170       5180       5190       5200       5210       5220 
SVFNILQATT ANVSALMGAN GNTIVDEEVK DMQFELYVNV YRKSQPDPKF VDRYYAFLNK 

      5230       5240       5250       5260       5270       5280 
HFSMMILSDD GVVCYNSDYA TKGYIASIQN FKETLYYQNN VFMSEAKCWV ETDLKKGPHE 

      5290       5300       5310       5320       5330       5340 
FCSQHTLFIK DGDDGYFLPY PDPSRILSAG CFVDDIVKTD GTLMVERFVS LAIDAYPLTK 

      5350       5360       5370       5380       5390       5400 
HDDPEYQNVF WVYLQYIEKL YKDLTGHMLD SYSVMLCGDN SAKFWEESFY RDLYTAPTTL 

      5410       5420       5430       5440       5450       5460 
QAVGSCVVCH SQTSLRCGTC IRRPFLCCKC CYDHVIATPH KMVLSVSPYV CNAPGCDVAD 

      5470       5480       5490       5500       5510       5520 
VTKLYLGGMS YFCIDHRPVC SFPLCANGLV FGLYKNMCTG SPSVTEFNRL ATCDWTESGD 

      5530       5540       5550       5560       5570       5580 
YTLANTTTEP LKLFAAETLR ATEEASKQSY AIATIKEIVG ERELLLVWEA GKAKPPLNRN 

      5590       5600       5610       5620       5630       5640 
YVFTGYHITK NSKVQLGEYV FERIDYSDAV SYKSSTTYKL AVGDIFVLTS HSVATLQAPT 

      5650       5660       5670       5680       5690       5700 
IVNQERYVKI TGLYPTLTVP EEFANHVANF QKAGFSKFVT VQGPPGTGKS HFAIGLAIYY 

      5710       5720       5730       5740       5750       5760 
PTARVVYTAC SHAAVDALCE KAFKYLNIAK CSRIIPAKAR VECYDQFKVN ETNSQYLFST 

      5770       5780       5790       5800       5810       5820 
INALPETSAD ILVVDEVSMC TNYDLSVINA RIKAKHIVYV GDPAQLPAPR TLLTRGTLEP 

      5830       5840       5850       5860       5870       5880 
ENFNSVTRLM CNLGPDIFLS VCYRCPEEIV NTVSALVYNN KLVAKKPASG QCFKILYKGS 

      5890       5900       5910       5920       5930       5940 
VTHDASSAIN RPQLNFVKSF IAANPNWSKA VFISPYNSQN AVARSVLGLT TQTVDSSQGS 

      5950       5960       5970       5980       5990       6000 
EYPYVIFCQT ADTAHANNIN RFNVAVTRAQ KGILCVMTSQ ALFDSLEFAE VSLNNYKLQS 

      6010       6020       6030       6040       6050       6060 
QIVTGLYKDC SRESSGLHPA YAPTYVSVDD KYKTSDELCV NLNVPANVPY SRVISRMGFK 

      6070       6080       6090       6100       6110       6120 
LDASIPNYPK LFITRDEAIR QVRSWIGFDV EGAHASRNAC GTNVPLQLGF STGVNFVVQP 

      6130       6140       6150       6160       6170       6180 
VGVVDTEWGS MLTSIAARPP PGEQFKHLVP LMNKGAAWPI VRRRIVQMLS DTLDKLSDYC 

      6190       6200       6210       6220       6230       6240 
TFVCWAHGFE LTSASYFCKI GKEQRCCMCN RRASTYSSPL HSYACWSHSS GYDYVYNPFF 

      6250       6260       6270       6280       6290       6300 
VDVQQWGYIG NLATNHDRYC SVHQGAHVAS NDAVMTRCLA IHDCFIERVE WDITYPYISH 

      6310       6320       6330       6340       6350       6360 
EKRLNSCCRA VERNVVRAAL LAGRFERVYD IGNPKGIPIV DDPVVDWHYY DAQPLSKKVQ 

      6370       6380       6390       6400       6410       6420 
QLFYTEDCAK NFSDGLCLFW NCNVPRYPNN AIVCRFDTRV HSEFNLPGCD GGSLYVNKHA 

      6430       6440       6450       6460       6470       6480 
FHTPAYDASA FRDLKPLPFF YYSTTPCEVH GNGNMLEDID YVPLKSAVCI TACNLGGAVC 

      6490       6500       6510       6520       6530       6540 
RKHAAEYRDY MEAYNLVSAS GFRLWCYKTF DVYNLWSTFT KIQGLENIAY NVIKQGHFTG 

      6550       6560       6570       6580       6590       6600 
VEGELPVAVV NDKIYTKSDV NDVCIFENKT TLPTNIAFEL YAKRAVRSHP DFNLLRNLEV 

      6610       6620       6630       6640       6650       6660 
DVCYKFVLWD YERSNIYGSA TIGVCKYTDI DVNSALNICF DIRDNGSLER FMSLPNGILI 

      6670       6680       6690       6700       6710       6720 
SDRKVKNYPC IVSSNYAYFN GTLIRDNTGN SQSSDGEVKQ PVTFYIYKKV NNEFVQFTDT 

      6730       6740       6750       6760       6770       6780 
YYTLGRTVSD FTPVSEMEKD FLALDSDVFI KKYKLEAYAF EHVVYGDFSR TTLGGLHLLI 

      6790       6800       6810       6820       6830       6840 
GLYKKHQEGH IIMEEMLKER ATVHNYFVTE SNTASFKAVC SVIDLKLDDF VDIIKAMDLS 

      6850       6860       6870       6880       6890       6900 
VVSKVVKIPI DLTMIEFMLW CKDGQVQTFY PRLQAINDWK PGLAMPSLFK VQNSNLEPCM 

      6910       6920       6930       6940       6950       6960 
LPNYKQSIPM PQGVHMNIAK YMQLCQYLNT CTIAVPANMR VMHFGAGSDK GVAPGSSVLR 

      6970       6980       6990       7000       7010       7020 
QWLPTDAILI DNDLNEYVSD ADITLFGDCV TVRVGQQVDL LISDMYDPST KVVGETNEAK 

      7030       7040       7050       7060       7070       7080 
ALFFVYLCNF IKNNLALGGS VAIKITEHSW SAELYELMGR FAWWTVFCTN ANASSSEGFL 

      7090       7100       7110       7120       7130       7140 
IGINYLGELK EVIDGNVMHA NYIFWRNTTL MNLSTYSLFD LSRFPLKLKG TPVLQLKESQ 

      7150       7160       7170       7180 
INELVISLLS QGKLIIRDND TLSVSTDVLV NFYRKPHKRS KC 

« Hide

Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6T5.

References

[1]"Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features."
Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y.
J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HKU5-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF065509 Genomic RNA. Translation: ABN10874.1.
RefSeqYP_001039961.1. NC_009020.1.

3D structure databases

ProteinModelPortalP0C6W4.
SMRP0C6W4. Positions 3937-4007, 4268-4328, 4335-4459.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0C6W4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4836003.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_BCHK5
AccessionPrimary (citable) accession number: P0C6W4
Secondary accession number(s): A3EXC9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries