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P0C6W4

- R1AB_BCHK5

UniProt

P0C6W4 - R1AB_BCHK5

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Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity
Host translation inhibitor nsp1: Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.By similarity
Non-structural protein 2: May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.By similarity
Papain-like proteinase: Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.By similarity
Non-structural protein 4: Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.By similarity
Proteinase 3CL-PRO: Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).By similarityPROSITE-ProRule annotation
Non-structural protein 6: Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.By similarity
Non-structural protein 7: Forms an hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity
Non-structural protein 8: Forms an hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity
Non-structural protein 9: May participate in viral replication by acting as a ssRNA-binding protein.By similarity
Non-structural protein 10: Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.By similarity
RNA-directed RNA polymerase: Responsible for replication and transcription of the viral RNA genome.By similarity
Helicase: Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.By similarity
Guanine-N7 methyltransferase: Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.By similarity
Uridylate-specific endoribonuclease: Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
2'-O-methyltransferase: Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei195 – 1962CleavageSequence Analysis
Sitei851 – 8522Cleavage; by PL-PROSequence Analysis
Active sitei1668 – 16681For PL-PRO activityPROSITE-ProRule annotation
Active sitei1838 – 18381For PL-PRO activityPROSITE-ProRule annotation
Sitei2830 – 28312Cleavage; by PL-PROSequence Analysis
Sitei3338 – 33392Cleavage; by 3CL-PROSequence Analysis
Active sitei3379 – 33791For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3486 – 34861For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3644 – 36452Cleavage; by 3CL-PROSequence Analysis
Sitei3936 – 39372Cleavage; by 3CL-PROSequence Analysis
Sitei4019 – 40202Cleavage; by 3CL-PROSequence Analysis
Sitei4218 – 42192Cleavage; by 3CL-PROSequence Analysis
Sitei4328 – 43292Cleavage; by 3CL-PROSequence Analysis
Sitei4467 – 44682Cleavage; by 3CL-PROSequence Analysis
Sitei5401 – 54022Cleavage; by 3CL-PROSequence Analysis
Sitei5999 – 60002Cleavage; by 3CL-PROSequence Analysis
Sitei6523 – 65242Cleavage; by 3CL-PROSequence Analysis
Sitei6874 – 68752Cleavage; by 3CL-PROSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1748 – 178538C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4402 – 441817By similarityAdd
BLAST
Zinc fingeri4444 – 445714By similarityAdd
BLAST
Nucleotide bindingi5683 – 56908ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. methyltransferase activity Source: InterPro
  7. omega peptidase activity Source: InterPro
  8. RNA binding Source: UniProtKB-KW
  9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host gene expression Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral protein processing Source: InterPro
  9. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Inhibition of host NF-kappa-B by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Papain-like proteinase (EC:3.4.19.12, EC:3.4.22.69)
Short name:
PL-PRO
Alternative name(s):
Non-structural protein 3
Short name:
nsp3
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)
Taxonomic identifieri694008 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiPipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus) [TaxID: 105295]
ProteomesiUP000007451: Genome

Subcellular locationi

Chain Papain-like proteinase : Host membrane; Multi-pass membrane protein. Host cytoplasm By similarity
Chain Non-structural protein 4 : Host membrane; Multi-pass membrane protein. Host cytoplasm
Note: Localizes in virally-induced cytoplasmic double-membrane vesicles.By similarity
Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2158 – 217821HelicalSequence AnalysisAdd
BLAST
Transmembranei2196 – 221621HelicalSequence AnalysisAdd
BLAST
Transmembranei2268 – 228821HelicalSequence AnalysisAdd
BLAST
Transmembranei2372 – 239221HelicalSequence AnalysisAdd
BLAST
Transmembranei2396 – 241621HelicalSequence AnalysisAdd
BLAST
Transmembranei2421 – 244121HelicalSequence AnalysisAdd
BLAST
Transmembranei2848 – 286821HelicalSequence AnalysisAdd
BLAST
Transmembranei3119 – 313921HelicalSequence AnalysisAdd
BLAST
Transmembranei3152 – 317221HelicalSequence AnalysisAdd
BLAST
Transmembranei3203 – 322321HelicalSequence AnalysisAdd
BLAST
Transmembranei3650 – 367021HelicalSequence AnalysisAdd
BLAST
Transmembranei3684 – 370421HelicalSequence AnalysisAdd
BLAST
Transmembranei3709 – 372921HelicalSequence AnalysisAdd
BLAST
Transmembranei3760 – 377718HelicalSequence AnalysisAdd
BLAST
Transmembranei3782 – 380221HelicalSequence AnalysisAdd
BLAST
Transmembranei3823 – 384321HelicalSequence AnalysisAdd
BLAST
Transmembranei3855 – 387521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Host translation inhibitor nsp1By similarityPRO_0000290303Add
BLAST
Chaini196 – 851656Non-structural protein 2By similarityPRO_0000290304Add
BLAST
Chaini852 – 28301979Papain-like proteinaseBy similarityPRO_0000290305Add
BLAST
Chaini2831 – 3338508Non-structural protein 4By similarityPRO_0000290306Add
BLAST
Chaini3339 – 36443063C-like proteinaseBy similarityPRO_0000290307Add
BLAST
Chaini3645 – 3936292Non-structural protein 6By similarityPRO_0000290308Add
BLAST
Chaini3937 – 401983Non-structural protein 7By similarityPRO_0000290309Add
BLAST
Chaini4020 – 4218199Non-structural protein 8By similarityPRO_0000290310Add
BLAST
Chaini4219 – 4328110Non-structural protein 9By similarityPRO_0000290311Add
BLAST
Chaini4329 – 4467139Non-structural protein 10By similarityPRO_0000290312Add
BLAST
Chaini4468 – 5401934RNA-directed RNA polymeraseBy similarityPRO_0000290313Add
BLAST
Chaini5402 – 5999598HelicaseBy similarityPRO_0000290314Add
BLAST
Chaini6000 – 6523524Guanine-N7 methyltransferaseBy similarityPRO_0000290315Add
BLAST
Chaini6524 – 6874351Uridylate-specific endoribonucleaseBy similarityPRO_0000290316Add
BLAST
Chaini6875 – 71823082'-O-methyltransferaseBy similarityPRO_0000290317Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Proteomic databases

PRIDEiP0C6W4.

Interactioni

Subunit structurei

Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6W4.
SMRiP0C6W4. Positions 3937-4007, 4268-4328, 4335-4459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1186 – 1345160MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1628 – 1902275Peptidase C16PROSITE-ProRule annotationAdd
BLAST
Domaini3339 – 3644306Peptidase C30PROSITE-ProRule annotationAdd
BLAST
Domaini5081 – 5243163RdRp catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini5402 – 548584CV MBDAdd
BLAST
Domaini5658 – 5839182(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini5840 – 6014175(+)RNA virus helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2158 – 2441284HD1By similarityAdd
BLAST
Regioni2848 – 3223376HD2By similarityAdd
BLAST
Regioni3650 – 3875226HD3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi964 – 1066103Glu-richAdd
BLAST
Compositional biasi5406 – 543126Cys-richAdd
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1748 – 178538C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4402 – 441817By similarityAdd
BLAST
Zinc fingeri4444 – 445714By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W4-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MSFVAGVAPQ GARGKYRAEL NTEKRTDHVS LKASLCDAGD LVLKISPWFM
60 70 80 90 100
DGESAYKHVS EQLSKGSKLL FVPQTLKGFI RHLPGPRVYL VERLTGGTYS
110 120 130 140 150
DPFMVNQLAY QNAAGEGVIG TTLQGKRVGM FFPFDADLVT GEFQFLLRKK
160 170 180 190 200
GFGGNRFRDA PWDYNWTPYS DLMDALEADP CGKYSQSLLK KLVGGDFTPI
210 220 230 240 250
DQYMCGKNGK PIAEFAALMA SEGITKLADV EAEVKSRTDS DRYIVFKNKL
260 270 280 290 300
YRIVWNVQRK DVAYSKQSAF TMNSIVQLDT MEDVPRHSFT IGSEIQVIAP
310 320 330 340 350
STAVQANGHL NLKQRLLYAF YGKQAVSEPN YIYHSAYVDC TSCGKGSWLT
360 370 380 390 400
GNAVQGFACD CGAHYCANDV DLQSSGLVRK NAVLLTTCPC NKDGECKHTL
410 420 430 440 450
PQLVSMMTDK CDVEVVGKTF ILTYGGVIYA YMGCSGGTMH FIPRAKSCVS
460 470 480 490 500
KIGDAIFTGC TGTWSKVCET ANLFLERAQH AINFVNEFVL TETVVALLSG
510 520 530 540 550
TTSSIEELRD LCRNATFEKV RDYLTPRGWI VTMGSYIEGV INVGAAGVCN
560 570 580 590 600
AALNAPFIVL SGLGESFKKV AATPWKLCSS LRETLDHYAD SITYRVFPYD
610 620 630 640 650
IPCDVTDYTA LLLDCAVLTG ASAYFVARYV DEKVEQLTNL VFSSCQSAVA
660 670 680 690 700
AFVQACMSTY KATAKFISDM FTLIKVVSER LYVYTSVGFV VVGDYSSQLL
710 720 730 740 750
KQFMHILSKA MQLLHTTVSW AGSKLPSVVY NGRDSLVFPS GTYYCVSTQG
760 770 780 790 800
RSLQDQFDLV IPGDLSKKQI GILEPTPNST TVDKKINTNV VEVVVGQLEP
810 820 830 840 850
TKEHSPELVV GDYVIISNKI FVRSVEDSET VFYPLCTDGK IVPTLFRLKG
860 870 880 890 900
GAPPKGVKFG GEQTKEITAV RSVSVDYDVH PVLDALLAGS ELATFTVEKD
910 920 930 940 950
LPVKDFVDVV KDEVIELLSK LLRGYNVDGF DLEDFADTPC YVYNAEGDLA
960 970 980 990 1000
WSSTMTFSVN PVEEVEEECD DDYVEDEYLS EEMLVEEDEN SWAAAVEAVI
1010 1020 1030 1040 1050
PMEDVQLDTL VAEIDVSEPA DDVAEQASTE EVEVPSACVL EASQVANAAE
1060 1070 1080 1090 1100
VESCEAEVSS SIPLHEDANA AKANDCAEGM PALDSTETVS KLSVDTPVGD
1110 1120 1130 1140 1150
VTQDDATSSN ATVISEDVHT ATHSKGLVAV PEVVPEKALG TSVERMRSTS
1160 1170 1180 1190 1200
EWTVVETSLK QETAVIVKND SSAKPQRVKK PKAENPLKNF KHIVLNNDVT
1210 1220 1230 1240 1250
LVFGDAIAVA RATEDCILVN AANTHLKHGG GIAAAIDRAS GGLVQAESDD
1260 1270 1280 1290 1300
YVNFYGPLNV GDSTLLKGHG LATGILHVVG PDARANQDIQ LLKRCYKAFN
1310 1320 1330 1340 1350
KYPLVVSPLI SAGIFCVEPR VSLEYLLSVV HTKTYVVVNS EKVYNDLAAP
1360 1370 1380 1390 1400
KPPTGLTYSH EGWRGIIRNA KSFGFTCFIC TDQSANAKLL KGRGVDLTKK
1410 1420 1430 1440 1450
TQTVDGVKYY LYSSKDPLTD IITAANACKG ICAMPIGYVT HGLDLAQAGQ
1460 1470 1480 1490 1500
QVKKITVPYV CLLASKDQVP ILNSDVAVQT PEQSFINTVI ANGGYHCWHL
1510 1520 1530 1540 1550
VTGELIVKGV SYRKLLNWSD QTICYADNKF YVVKGQIALP FDSLEKCRTY
1560 1570 1580 1590 1600
LTSRAAQQKN VDVLVTIDGV NFRTVVLNNT TTYRVQLGSV FYKGSDISDT
1610 1620 1630 1640 1650
IPTEKMSGEA VYLADNLSEA EKAVLSEVYG TADTAFLHRY YSLLALVKKW
1660 1670 1680 1690 1700
KYTVHDGVKS LKLNSNNCYV NVTMLMLDML KEIKFIVPAL QAAYLKHKGG
1710 1720 1730 1740 1750
DSTEFIALIM AYGDCTYGEP DDASRLLHTI LSKAELTTQA KMVWRQWCNV
1760 1770 1780 1790 1800
CGVQDTTTTG LKACIYVGMN SLDELHATHE ECCQCGDVRK RQLVEHNAPW
1810 1820 1830 1840 1850
LLLSGLNEAK VMTPTSQSAG PDYTAFNVFQ GVETSVGHYL HVRVKDNLLY
1860 1870 1880 1890 1900
KYDSGSLSKT SDMKCKMTDV YYPKQRYSAD CNVVVYSLDG NTWADVDPDL
1910 1920 1930 1940 1950
SAFYMKDGKY FTKKPVIEYS PATILSGSVY TNSCLVGHDG TIGSDAISSS
1960 1970 1980 1990 2000
FNNLLGFDNS KPVSKKLTYS FFPDFEGDVI LTEYSTYDPI YKNGAMLHGK
2010 2020 2030 2040 2050
PILWVNNSKF DSALNKFNRA TLRQVYDIAP VTLENKYTVL QDNQIQQVEV
2060 2070 2080 2090 2100
EAPKEDAKPQ SPVQVAEDID NKLPIIKCKG LKKPFVKDGY SFVNDPQGVN
2110 2120 2130 2140 2150
VIDTLGIDDL RALYVDRNLR LIVLKENNWS ALFNIHTVEK GDLSVIAASG
2160 2170 2180 2190 2200
SITRRVKILL GASSLFAQFA SVTVNVTTAM GKALGRMTRN VITNTGIIGQ
2210 2220 2230 2240 2250
GFALLKMLLI LPFTFWKSKN QSTVKVEVGA LRTAGIVTTN VVKQCASAAY
2260 2270 2280 2290 2300
DVLVVKFKRI DWKSTLRLLF LICTTGLLLS SLYYLFLFHQ VLTSDVMLDG
2310 2320 2330 2340 2350
AEGMLATYRE LRSYLGIHSL CDGMVEAYRN VSYDVNDFCS NRSALCNWCL
2360 2370 2380 2390 2400
IGQDSLTRYS AFQMIQTHVT SYVINIDWVW FVMEFALAYV LYTSTFNVLL
2410 2420 2430 2440 2450
LVVSSQYFFS YTGAFVNWRS YNYLVSGYFF CVTHIPLLGL VRIYNFLACL
2460 2470 2480 2490 2500
WFLRRFYNHV INGCKDTACL LCYKRNRLTR VEASTVVCGS KRTFYIVANG
2510 2520 2530 2540 2550
GTSFCCRHNW NCVDCDTAGI GNTFICEEVA NDLTTSLRRL VKPTDKSHYY
2560 2570 2580 2590 2600
VESVTVKDSV VQLHYSREGA SCYERYPLCY FTNLDKLKFK EVCKTPTGIP
2610 2620 2630 2640 2650
EHNFLIYDSS DRGQENLARS ACVYYSQVLS KPMLLVDSNM VTTVGDSREI
2660 2670 2680 2690 2700
ASKMLDSYVN SFISLFGVNR DKLDKLVATA RDCVKRGDDF QTVIKTFTDA
2710 2720 2730 2740 2750
ARGPAGVESD VETSSIVDAL QYAYKHDLQL TTEGFNNYVP SYIKPDSVAT
2760 2770 2780 2790 2800
ADLGCLIDLN AASVNQTSIR NANGACIWNS SDYMKLSDSL KRQIRIACRK
2810 2820 2830 2840 2850
CNIPFRLTTS RLRSADNILS VKFSATKLSG GAPKWLLKLR DFTWKSYCVV
2860 2870 2880 2890 2900
TLVVFAMAVL SYLCLPAFNM SQVSFHEDRI LTYKVVENGI IRDITPSDTC
2910 2920 2930 2940 2950
FANKYQSFSK WFNEHYGGLF NNDISCPVTV AVIAGVAGAR VPNLPANVAW
2960 2970 2980 2990 3000
VGRQIVLFVS RVFASSNNVC YTPTAEIPYE RFSDSGCVLA SECTLFRDAE
3010 3020 3030 3040 3050
GKINPYCYDP TVLPGASAYD QMKPHVRYDM YDSDMYIKFP EVVFESTLRI
3060 3070 3080 3090 3100
TKTLATRYCR FGSCEDANEG VCITTNGSWA IYNDHYANKP GVYCGDNYFD
3110 3120 3130 3140 3150
IVRRLGLSLF QPVTYFQLST SLALGVMLCI FLTIAFYYVN KVKRALADYT
3160 3170 3180 3190 3200
QCAVVAVAAA LLNSLCLCFV VSNPLLVLPY TALYYYATFY LTGEPAFVMH
3210 3220 3230 3240 3250
VSWFVMFGTV VPIWMVFAYI VGVCLRHLLW VMAYFSKKHV EVFTDGKLNC
3260 3270 3280 3290 3300
SFQDAAANIF VINKDTYVAL RNSITQDSYN RYLSMFNKYK YYSGAMDTAS
3310 3320 3330 3340 3350
YREASAAHLC KALQVYSETG SDVLFQPPNC SVTSSVLQSG LVKMAAPSGV
3360 3370 3380 3390 3400
VENCMVQVTC GSMTLNGLWL DNYVWCPRHV MCPADQLSDP NYDALLVSKT
3410 3420 3430 3440 3450
NLSFIVQKNV GAPANLRVVG HTMVGTLLKL TVESANPQTP AYTFTTVKPG
3460 3470 3480 3490 3500
ASFSVLACYN GRPTGVFMVN MRQNSTIKGS FLCGSCGSVG YTQEGNVINF
3510 3520 3530 3540 3550
CYMHQMELSN GTHTGCAFDG VMYGAFEDRQ VHQVQLSDKY CTINIVAWLY
3560 3570 3580 3590 3600
AAILNGCNWF VKPNKTGIAT FNEWAMSNQF TEFIGTQSVD MLAHKTGVSV
3610 3620 3630 3640 3650
EQLLYAIQTL HKGFQGKTIL GNSMLEDEFT PDDVNMQVMG VVMQSGVKRI
3660 3670 3680 3690 3700
SYGLVHWLFT TLLLAYVATL QLTKFTIWNY LFEVIPLQLT PLVLCVMACV
3710 3720 3730 3740 3750
MLTVKHKHTF LTLFLLPTAI CLTYANIVYE PQTPVSSALI AVANWLNPAS
3760 3770 3780 3790 3800
VYMRTTHTDL GVYLSLCFAL AVVVRRLYRP NASNLALALG SAMVWFYTYT
3810 3820 3830 3840 3850
TGDCSSPLTY LMFLTTLTSD YTVTVFLAVN VAKFFARVVF LYAPHAGFIF
3860 3870 3880 3890 3900
PEVKLVLLMY LAVGYFCTVY FGVFSLLNLK LRVPLGVYDY TVSTQEFRYL
3910 3920 3930 3940 3950
TGNGLHAPRN SWEALRLNMK LIGIGGTPCI KIASVQSKLT DLKCTSVVLL
3960 3970 3980 3990 4000
SVLQQLHLEA NSKAWAHCVK LHNDILAATD PTEAFDNFVC LFATLMSFSA
4010 4020 4030 4040 4050
NVDLEALASD LLDHPSVLQA TLSEFSHLAS YAELEAAQSS YQKALNSGDA
4060 4070 4080 4090 4100
SPQVLKALQK AVNIAKNAYE KDKAVARKLE RMAEQAMTSM YKQARAEDKK
4110 4120 4130 4140 4150
AKIVSAMQTM LFGMIKKLDN DVLNGVISNA RNGCVPLSVV PLCASNKLRV
4160 4170 4180 4190 4200
VIPDITIWNK VVTWPSLSYA GALWDISLIN NVDGEVVKSS DVTETNESLT
4210 4220 4230 4240 4250
WPLVLECTRA ASSAVTLQNN EIRPSGLKTM VVSAGIDHAN CNTSSLAYYE
4260 4270 4280 4290 4300
PVEGRKMLMG ILSENAHLKW AKVEGRDGFV NIELQPPCKF LIAGPKGPEV
4310 4320 4330 4340 4350
RYLYFVKNLN NLHRGQLLGH IAATVRLQAG SNTEFAINSS VLSAVTFSVD
4360 4370 4380 4390 4400
PGKAYLDFVN AGGAPLTNCV KMLTPKTGTG IAVSVKPEAN ADQDTYGGAS
4410 4420 4430 4440 4450
VCLYCRAHIE HPDVTGVCKF KGKFVQVPLH IRDPVGFCLQ NTPCNVCQFW
4460 4470 4480 4490 4500
IGHGCNCDAL RGTTIPQSKD SNFLNRVRGS IVNARIEPCA SGLTTDVVFR
4510 4520 4530 4540 4550
AFDICNYKAK VAGIGKYYKT NTCRFVEVDD EGHRLDSFFV VKRHTMENYE
4560 4570 4580 4590 4600
LEKRCYDLVK DCDAVAVHDF FIFDVDKVKT PHIVRQRLTE YTMMDLVYAL
4610 4620 4630 4640 4650
RHFDQNNCEV LKSILVKYGC CDASYFDNKL WFDFVENPNV ISVYHKLGER
4660 4670 4680 4690 4700
IRQAVLNTVK FCDQMVKSGL VGVLTLDNQD LNGKWYDFGD FVITQPGAGV
4710 4720 4730 4740 4750
AIVDSYYSYL MPVLSMTNCL AAETHRDCDL TKPLIEWPLL EYDYTDYKIG
4760 4770 4780 4790 4800
LFEKYFKXWD QQYHPNCVNC TDDRCVLHCA NFNVLFSMTL PGTSFGPIVR
4810 4820 4830 4840 4850
KIFVDGVPFV ISCGYHYKEL GLVMNMDVSL HRHRLSLKEL MMYAADPAMH
4860 4870 4880 4890 4900
IASASALWDL RTPCFSVAAL TTGLTFQTVR PGNFNKDFYD FVVSKGFFKE
4910 4920 4930 4940 4950
GSSVTLRHFF FAQDGHAAIT DYSYYAYNLP TMCDIKQMLF CMEVVDRYFE
4960 4970 4980 4990 5000
IYDGGCLNAS EVIVNNLDKS AGHPFNKFGK ARVYYESLSY QEQDELFAMT
5010 5020 5030 5040 5050
KRNVLPTITQ MNLKYAISAK NRARTVAGVS ILSTMTNRQY HQKMLKSMAA
5060 5070 5080 5090 5100
TRGSTCVIGT TKFYGGWDFM LKTLYKDVDN PHLMGWDYPK CDRAMPNMCR
5110 5120 5130 5140 5150
IFASLILARK HSTCCTNTDR FYRLANECAQ VLSEYVLCGG GYYVKPGGTS
5160 5170 5180 5190 5200
SGDATTAYAN SVFNILQATT ANVSALMGAN GNTIVDEEVK DMQFELYVNV
5210 5220 5230 5240 5250
YRKSQPDPKF VDRYYAFLNK HFSMMILSDD GVVCYNSDYA TKGYIASIQN
5260 5270 5280 5290 5300
FKETLYYQNN VFMSEAKCWV ETDLKKGPHE FCSQHTLFIK DGDDGYFLPY
5310 5320 5330 5340 5350
PDPSRILSAG CFVDDIVKTD GTLMVERFVS LAIDAYPLTK HDDPEYQNVF
5360 5370 5380 5390 5400
WVYLQYIEKL YKDLTGHMLD SYSVMLCGDN SAKFWEESFY RDLYTAPTTL
5410 5420 5430 5440 5450
QAVGSCVVCH SQTSLRCGTC IRRPFLCCKC CYDHVIATPH KMVLSVSPYV
5460 5470 5480 5490 5500
CNAPGCDVAD VTKLYLGGMS YFCIDHRPVC SFPLCANGLV FGLYKNMCTG
5510 5520 5530 5540 5550
SPSVTEFNRL ATCDWTESGD YTLANTTTEP LKLFAAETLR ATEEASKQSY
5560 5570 5580 5590 5600
AIATIKEIVG ERELLLVWEA GKAKPPLNRN YVFTGYHITK NSKVQLGEYV
5610 5620 5630 5640 5650
FERIDYSDAV SYKSSTTYKL AVGDIFVLTS HSVATLQAPT IVNQERYVKI
5660 5670 5680 5690 5700
TGLYPTLTVP EEFANHVANF QKAGFSKFVT VQGPPGTGKS HFAIGLAIYY
5710 5720 5730 5740 5750
PTARVVYTAC SHAAVDALCE KAFKYLNIAK CSRIIPAKAR VECYDQFKVN
5760 5770 5780 5790 5800
ETNSQYLFST INALPETSAD ILVVDEVSMC TNYDLSVINA RIKAKHIVYV
5810 5820 5830 5840 5850
GDPAQLPAPR TLLTRGTLEP ENFNSVTRLM CNLGPDIFLS VCYRCPEEIV
5860 5870 5880 5890 5900
NTVSALVYNN KLVAKKPASG QCFKILYKGS VTHDASSAIN RPQLNFVKSF
5910 5920 5930 5940 5950
IAANPNWSKA VFISPYNSQN AVARSVLGLT TQTVDSSQGS EYPYVIFCQT
5960 5970 5980 5990 6000
ADTAHANNIN RFNVAVTRAQ KGILCVMTSQ ALFDSLEFAE VSLNNYKLQS
6010 6020 6030 6040 6050
QIVTGLYKDC SRESSGLHPA YAPTYVSVDD KYKTSDELCV NLNVPANVPY
6060 6070 6080 6090 6100
SRVISRMGFK LDASIPNYPK LFITRDEAIR QVRSWIGFDV EGAHASRNAC
6110 6120 6130 6140 6150
GTNVPLQLGF STGVNFVVQP VGVVDTEWGS MLTSIAARPP PGEQFKHLVP
6160 6170 6180 6190 6200
LMNKGAAWPI VRRRIVQMLS DTLDKLSDYC TFVCWAHGFE LTSASYFCKI
6210 6220 6230 6240 6250
GKEQRCCMCN RRASTYSSPL HSYACWSHSS GYDYVYNPFF VDVQQWGYIG
6260 6270 6280 6290 6300
NLATNHDRYC SVHQGAHVAS NDAVMTRCLA IHDCFIERVE WDITYPYISH
6310 6320 6330 6340 6350
EKRLNSCCRA VERNVVRAAL LAGRFERVYD IGNPKGIPIV DDPVVDWHYY
6360 6370 6380 6390 6400
DAQPLSKKVQ QLFYTEDCAK NFSDGLCLFW NCNVPRYPNN AIVCRFDTRV
6410 6420 6430 6440 6450
HSEFNLPGCD GGSLYVNKHA FHTPAYDASA FRDLKPLPFF YYSTTPCEVH
6460 6470 6480 6490 6500
GNGNMLEDID YVPLKSAVCI TACNLGGAVC RKHAAEYRDY MEAYNLVSAS
6510 6520 6530 6540 6550
GFRLWCYKTF DVYNLWSTFT KIQGLENIAY NVIKQGHFTG VEGELPVAVV
6560 6570 6580 6590 6600
NDKIYTKSDV NDVCIFENKT TLPTNIAFEL YAKRAVRSHP DFNLLRNLEV
6610 6620 6630 6640 6650
DVCYKFVLWD YERSNIYGSA TIGVCKYTDI DVNSALNICF DIRDNGSLER
6660 6670 6680 6690 6700
FMSLPNGILI SDRKVKNYPC IVSSNYAYFN GTLIRDNTGN SQSSDGEVKQ
6710 6720 6730 6740 6750
PVTFYIYKKV NNEFVQFTDT YYTLGRTVSD FTPVSEMEKD FLALDSDVFI
6760 6770 6780 6790 6800
KKYKLEAYAF EHVVYGDFSR TTLGGLHLLI GLYKKHQEGH IIMEEMLKER
6810 6820 6830 6840 6850
ATVHNYFVTE SNTASFKAVC SVIDLKLDDF VDIIKAMDLS VVSKVVKIPI
6860 6870 6880 6890 6900
DLTMIEFMLW CKDGQVQTFY PRLQAINDWK PGLAMPSLFK VQNSNLEPCM
6910 6920 6930 6940 6950
LPNYKQSIPM PQGVHMNIAK YMQLCQYLNT CTIAVPANMR VMHFGAGSDK
6960 6970 6980 6990 7000
GVAPGSSVLR QWLPTDAILI DNDLNEYVSD ADITLFGDCV TVRVGQQVDL
7010 7020 7030 7040 7050
LISDMYDPST KVVGETNEAK ALFFVYLCNF IKNNLALGGS VAIKITEHSW
7060 7070 7080 7090 7100
SAELYELMGR FAWWTVFCTN ANASSSEGFL IGINYLGELK EVIDGNVMHA
7110 7120 7130 7140 7150
NYIFWRNTTL MNLSTYSLFD LSRFPLKLKG TPVLQLKESQ INELVISLLS
7160 7170 7180
QGKLIIRDND TLSVSTDVLV NFYRKPHKRS KC

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,182
Mass (Da):799,932
Last modified:June 10, 2008 - v1
Checksum:i455E36D8EF6FD1E1
GO
Isoform Replicase polyprotein 1a (identifier: P0C6T5-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6T5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,481
Mass (Da):494,801
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065509 Genomic RNA. Translation: ABN10874.1.
RefSeqiYP_001039961.1. NC_009020.1. [P0C6W4-1]

Genome annotation databases

GeneIDi4836003.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065509 Genomic RNA. Translation: ABN10874.1 .
RefSeqi YP_001039961.1. NC_009020.1. [P0C6W4-1 ]

3D structure databases

ProteinModelPortali P0C6W4.
SMRi P0C6W4. Positions 3937-4007, 4268-4328, 4335-4459.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0C6W4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4836003.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features."
    Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y.
    J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HKU5-1.

Entry informationi

Entry nameiR1AB_BCHK5
AccessioniPrimary (citable) accession number: P0C6W4
Secondary accession number(s): A3EXC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3