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P0C6W3 (R1AB_BCHK4) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    Leader protein
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65 homolog
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name=PL-PRO
  4. Non-structural protein 4
    Short name=nsp4
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    nsp5
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
  8. Non-structural protein 8
    Short name=nsp8
  9. Non-structural protein 9
    Short name=nsp9
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismBat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004) [Complete proteome]
Taxonomic identifier694007 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostTylonycteris pachypus (Lesser bamboo bat) [TaxID: 258959]

Protein attributes

Sequence length7119 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 1 peptidase C16 domain.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Eukaryotic host gene expression shutoff by virus
Eukaryotic host translation shutoff by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6W3-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6T4-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6T4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Non-structural protein 1 Potential
PRO_0000290288
Chain196 – 847652Non-structural protein 2 Potential
PRO_0000290289
Chain848 – 27841937Non-structural protein 3 Potential
PRO_0000290290
Chain2785 – 3291507Non-structural protein 4 Potential
PRO_0000290291
Chain3292 – 35973063C-like proteinase Potential
PRO_0000290292
Chain3598 – 3889292Non-structural protein 6 Potential
PRO_0000290293
Chain3890 – 397283Non-structural protein 7 Potential
PRO_0000290294
Chain3973 – 4171199Non-structural protein 8 Potential
PRO_0000290295
Chain4172 – 4281110Non-structural protein 9 Potential
PRO_0000290296
Chain4282 – 4420139Non-structural protein 10 Potential
PRO_0000290297
Chain4421 – 5354934RNA-directed RNA polymerase Potential
PRO_0000290298
Chain5355 – 5952598Helicase Potential
PRO_0000290299
Chain5953 – 6475523Exoribonuclease Potential
PRO_0000290300
Chain6476 – 6817342Uridylate-specific endoribonuclease Potential
PRO_0000290301
Chain6818 – 7119302Putative 2'-O-methyl transferase Potential
PRO_0000290302

Regions

Transmembrane2112 – 213221Helical; Potential
Transmembrane2145 – 216521Helical; Potential
Transmembrane2222 – 224221Helical; Potential
Transmembrane2326 – 234621Helical; Potential
Transmembrane2350 – 237021Helical; Potential
Transmembrane2375 – 239521Helical; Potential
Transmembrane2800 – 282021Helical; Potential
Transmembrane3072 – 309221Helical; Potential
Transmembrane3105 – 312521Helical; Potential
Transmembrane3149 – 316921Helical; Potential
Transmembrane3603 – 362321Helical; Potential
Transmembrane3637 – 365721Helical; Potential
Transmembrane3662 – 368221Helical; Potential
Transmembrane3707 – 372721Helical; Potential
Transmembrane3735 – 375521Helical; Potential
Transmembrane3784 – 380421Helical; Potential
Transmembrane3808 – 382821Helical; Potential
Domain1152 – 1321170Macro
Domain1593 – 1864272Peptidase C16
Domain3292 – 3597306Peptidase C30
Domain5034 – 5196163RdRp catalytic
Domain5355 – 543884CV MBD
Domain5611 – 5792182(+)RNA virus helicase ATP-binding
Domain5793 – 5967175(+)RNA virus helicase C-terminal
Zinc finger1714 – 175138C4-type By similarity
Zinc finger4355 – 437117 By similarity
Zinc finger4397 – 441014 By similarity
Nucleotide binding5636 – 56438ATP By similarity
Region2112 – 2395284HD1 By similarity
Region2800 – 3169370HD2 By similarity
Region3603 – 3828226HD3 By similarity
Compositional bias960 – 104990Glu-rich
Compositional bias4161 – 41666Poly-Ser
Compositional bias5359 – 538426Cys-rich

Sites

Active site16341For PL-PRO activity By similarity
Active site18001For PL-PRO activity By similarity
Active site33321For 3CL-PRO activity By similarity
Active site34391For 3CL-PRO activity By similarity
Site195 – 1962Cleavage Potential
Site847 – 8482Cleavage; by PL-PRO Potential
Site2784 – 27852Cleavage; by PL-PRO Potential
Site3291 – 32922Cleavage; by 3CL-PRO Potential
Site3597 – 35982Cleavage; by 3CL-PRO Potential
Site3889 – 38902Cleavage; by 3CL-PRO Potential
Site3972 – 39732Cleavage; by 3CL-PRO Potential
Site4171 – 41722Cleavage; by 3CL-PRO Potential
Site4281 – 42822Cleavage; by 3CL-PRO Potential
Site4420 – 44212Cleavage; by 3CL-PRO Potential
Site5354 – 53552Cleavage; by 3CL-PRO Potential
Site5952 – 59532Cleavage; by 3CL-PRO Potential
Site6475 – 64762Cleavage; by 3CL-PRO Potential
Site6817 – 68182Cleavage; by 3CL-PRO Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: E85475854586DD79

FASTA7,119795,200
        10         20         30         40         50         60 
MLSKASVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTDD MACKLTPWFE DGETAFNQVS 

        70         80         90        100        110        120 
SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS KHFLVNQLAY KDQVGAAMMR 

       130        140        150        160        170        180 
TTLNAKPLGM FFPYDSSLET GEYTFLLRKN GLGGQLFRER PWDRKETPYV EILDDLEADP 

       190        200        210        220        230        240 
TGKYSQNLLK KLIGGDCIPI DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS 

       250        260        270        280        290        300 
DRFIVLNKKL YRVVWNVTRR NVPYPKQTAF TIVSVVQCDD KDSVPEHTFT IGSQILMVSP 

       310        320        330        340        350        360 
LKATNNKNFN LKQRLLYTFY GKDAVQQPGY IYHSAYVDCN ACGRGTWCTG NAIQGFACDC 

       370        380        390        400        410        420 
GANYSANDVD LQSSGLVPRN ALFLANCPCA NNGACSHSAA QVYNILDGKA CVEVGGKSFT 

       430        440        450        460        470        480 
LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR IGDAIFTGCT GTWDKVVETA NLFLEKAQRS 

       490        500        510        520        530        540 
LNFCQQFALT EVVLAILSGT TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI 

       550        560        570        580        590        600 
NIGANGVCNA TINAPFIAFT GLGESFKKVS AIPWKICSNL KSALDYYSSN IMFRVFPYDI 

       610        620        630        640        650        660 
PCDVSNFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SSACQTALSS FLNACVAASR 

       670        680        690        700        710        720 
ATAGFINDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ QVLDIMSKAM KLLHTNVSWA 

       730        740        750        760        770        780 
GTKLSAIIYE GREALLFNSG TYFCLSTKAK TLQGQMNLVL PGDYNKKTLG ILDPVPNADT 

       790        800        810        820        830        840 
IDVNANSTVV DVVHGQLEPT NEHGPSMIVG NYVLVSDKLF VRTEDEEFYP LCTNGKVVST 

       850        860        870        880        890        900 
LFRLKGGMPS KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE 

       910        920        930        940        950        960 
DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN MTFSINPVED 

       970        980        990       1000       1010       1020 
VEEVEEFIED DYLSDELPIA DDEEAWARAV EEVMPLDDIL VAEIELEEDP PLETALESVE 

      1030       1040       1050       1060       1070       1080 
AEVVETAEAQ EPSVESIDST PSTSTVVGEN DLSVKPMSRV AETDDVLELE TAVVGGPVSD 

      1090       1100       1110       1120       1130       1140 
VTAIVTNDIV SVEQAQQCGV SSLPIQDEAS ENQVHQVSDL QGNELLCSET KVEIVQPRQD 

      1150       1160       1170       1180       1190       1200 
LKPRRSRKSK VDLSKYKHTV INNSVTLVLG DAIQIASLLP KCILVNAANR HLKHGGGIAG 

      1210       1220       1230       1240       1250       1260 
VINKASGGDV QEESDEYISN NGPLHVGDSV LLKGHGLADA ILHVVGPDAR NNEDAALLKR 

      1270       1280       1290       1300       1310       1320 
CYKAFNKHTI VVTPLISAGI FSVDPKVSFE YLLANVTTTT YVVVNNEDIY NTLATPSKPD 

      1330       1340       1350       1360       1370       1380 
GLVYSFEGWR GTVRTAKNYG FTCFICTEYS ANVKFLRTKG VDTTKKIQTV DGVSYYLYSA 

      1390       1400       1410       1420       1430       1440 
RDALTDVIAA ANGCSGICAM PFGYVTHGLD LAQSGNYVRQ VKVPYVCLLA SKEQIPIMNS 

      1450       1460       1470       1480       1490       1500 
DVAIQTPETA FINNVTSNGG YHSWHLVSGD LIVKDVCYKK LLHWSGQTIC YADNKFYVVK 

      1510       1520       1530       1540       1550       1560 
NDVALPFSDL EACRAYLTSR AAQQVNIEVL VTIDGVNFRT VILNDTTTFR KQLGATFYKG 

      1570       1580       1590       1600       1610       1620 
VDISDAFPTV KMGGESLFVA DNLSESEKVV LKEYYGTSDV TFLQRYYSLQ PLVQQWKFVV 

      1630       1640       1650       1660       1670       1680 
HDGVKSLKLS NYNCYINATI MMIDMLHDIK FVVPALQNAY LRYKGGDPYD FLALIMAYGD 

      1690       1700       1710       1720       1730       1740 
CTFDNPDDEA KLLHTLLAKA ELTVSAKMVW REWCTVCGIR DIEYTGMRAC VYAGVNSMEE 

      1750       1760       1770       1780       1790       1800 
LQSVFNETCV CGSVKHRQLV EHSAPWLLVS GLNEVKVSTS TDPIYRAFNV FQGVETSVGH 

      1810       1820       1830       1840       1850       1860 
YVHIRVKDGL FYKYDSGSLT KTSDMKCKMT SVWYPTVRYT ADCNVVVYDL DGVTKVEVNP 

      1870       1880       1890       1900       1910       1920 
DLSNYYMKDG KYYTSKPTIK YSPATILPGS VYSNSCLVGV DGTPGSDTIS KFFNDLLGFD 

      1930       1940       1950       1960       1970       1980 
ETKPISKKLT YSLLPNEDGD VLLSEFSNYN PVYKKGVMLK GKPILWVNNG VCDSALNKPN 

      1990       2000       2010       2020       2030       2040 
RASLRQLYDV APIVLDNKYT VLQDNTSQLV EHNVPVVDDV PITTRKLIEV KCKGLNKPFV 

      2050       2060       2070       2080       2090       2100 
KGNFSFVNDP NGVTVVDTLG LTELRALYVD INTRYIVLRD NNWSSLFKLH TVESGDLQIV 

      2110       2120       2130       2140       2150       2160 
AAGGSVTRRA RVLLGASSLF ASFAKITVTA TTAACKTAGR GFCKFVVNYG VLQNMFVFLK 

      2170       2180       2190       2200       2210       2220 
MLFFLPFNYL WPKKQPTVDI GVSGLRTAGI VTTNIVKQCG TAAYYMLLGK FKRVDWKATL 

      2230       2240       2250       2260       2270       2280 
RLFLLLCTTI LLLSSIYHLV LFNQVLSSDV MLEDATGILA IYKEVRSYLG IRTLCDGLVV 

      2290       2300       2310       2320       2330       2340 
EYRNTSFDVM EFCSNRSVLC QWCLIGQDSL TRYSALQMLQ THITSYVLNI DWIWFALEFF 

      2350       2360       2370       2380       2390       2400 
LAYVLYTSSF NVLLLVVTAQ YFFAYTSAFV NWRAYNYIVS GLFFLVTHIP LHGLVRVYNF 

      2410       2420       2430       2440       2450       2460 
LACLWFLRKF YSHVINGCKD TACLLCYKRN RLTRVEASTI VCGTKRTFYI AANGGTSYCC 

      2470       2480       2490       2500       2510       2520 
KHNWNCVECD TAGVGNTFIC TEVANDLTTT LRRLIKPTDQ SHYYVDSVVV KDAVVELHYN 

      2530       2540       2550       2560       2570       2580 
RDGSSCYERY PLCYFTNLEK LKFKEVCKTP TGIPEHNFLI YDTNDRGQEN LARSACVYYS 

      2590       2600       2610       2620       2630       2640 
QVLCKPMLLV DVNLVTTVGD SREIAIKMLD SFINSFISLF SVSRDKLEKL INTARDCVRR 

      2650       2660       2670       2680       2690       2700 
GDDFQNVLKT FTDAARGHAG VESDVETTMV VDALQYAHKN DIQLTTECYN NYVPGYIKPD 

      2710       2720       2730       2740       2750       2760 
SINTLDLGCL IDLKAASVNQ TSMRNANGAC VWNSGDYMKL SDSFKRQIRI ACRKCNIPFR 

      2770       2780       2790       2800       2810       2820 
LTTSKLRAAD NILSVKFSAT KIVGGAPSWL LRVRDLTVKG YCILTLFVFT VAVLSWFCLP 

      2830       2840       2850       2860       2870       2880 
SYSIATVNFN DDRILTYKVI ENGIVRDIAP NDVCFANKYG HFSKWFNENH GGVYRNSMDC 

      2890       2900       2910       2920       2930       2940 
PITIAVIAGV AGARVANVPA NLAWVGKQIV LFVSRVFANT NVCFTPINEI PYDTFSDSGC 

      2950       2960       2970       2980       2990       3000 
VLSSECTLFR DAEGNLNPFC YDPTVLPGAS SYADMKPHVR YDMYDSDMYI KFPEVIVEST 

      3010       3020       3030       3040       3050       3060 
LRITKTLATQ YCRFGSCEES AAGVCISTNG SWALYNQNYS TRPGIYCGDD YFDIVRRLAI 

      3070       3080       3090       3100       3110       3120 
SLFQPVTYFQ LSTSLAMGLV LCVFLTAAFY YINKVKRALA DYTQCAVVAV VAALLNSLCL 

      3130       3140       3150       3160       3170       3180 
CFIVANPLLV APYTAMYYYA TFYLTGEPAF IMHISWYVMF GAVVPIWMLA SYTVGVMLRH 

      3190       3200       3210       3220       3230       3240 
LFWVLAYFSK KHVDVFTDGK LNCSFQDAAS NIFVIGKDTY VALRNAITQD SFVRYLSLFN 

      3250       3260       3270       3280       3290       3300 
KYKYYSGAMD TASYREACAA HLCKALQTYS ETGSDILYQP PNCSVTSSVL QSGLVKMSAP 

      3310       3320       3330       3340       3350       3360 
SGAVENCIVQ VTCGSMTLNG LWLDNTVWCP RHIMCPADQL TDPNYDALLI SKTNHSFIVQ 

      3370       3380       3390       3400       3410       3420 
KHIGAQANLR VVAHSMVGVL LKLTVDVANP STPAYTFSTV KPGASFSVLA CYNGKPTGVF 

      3430       3440       3450       3460       3470       3480 
TVNLRHNSTI KGSFLCGSCG SVGYTENGGV INFVYMHQME LSNGTHTGSS FDGVMYGAFE 

      3490       3500       3510       3520       3530       3540 
DKQTHQLQLT DKYCTINVVA WLYAAVLNGC KWFVKPTRVG IVTYNEWALS NQFTEFVGTQ 

      3550       3560       3570       3580       3590       3600 
SIDMLAHRTG VSVEQMLAAI QSLHAGFQGK TILGQSTLED EFTPDDVNMQ VMGVVMQSGV 

      3610       3620       3630       3640       3650       3660 
KRISYGFIHW LISTFVLAYV SVMQLTKFTM WTYLFETIPT QMTPLLLGFM ACVMFTVKHK 

      3670       3680       3690       3700       3710       3720 
HTFMSLFLLP VALCLTYANI VYEPQTLISS TLIAVANWLT PTSVYMRTTH FDFGLYISLS 

      3730       3740       3750       3760       3770       3780 
FVLAIIVRRL YRPSMSNLAL ALCSGVMWFY TYVIGDHSSP ITYLMFITTL TSDYTITVFA 

      3790       3800       3810       3820       3830       3840 
TVNLAKFISG LVFFYAPHLG FILPEVKLVL LIYLGLGYMC TMYFGVFSLL NLKLRVPLGV 

      3850       3860       3870       3880       3890       3900 
YDYSVSTQEF RFLTGNGLHA PRNSWEALIL NFKLLGIGGT PCIKVATVQS KLTDLKCTSV 

      3910       3920       3930       3940       3950       3960 
VLLTVLQQLH LESNSKAWSY CVKLHNEILA AVDPTEAFER FVCLFATLMS FSANVDLDAL 

      3970       3980       3990       4000       4010       4020 
ANDLFENSSV LQATLTEFSH LATYAELETA QSSYQKALNS GDASPQVLKA LQKAVNVAKN 

      4030       4040       4050       4060       4070       4080 
AYEKDKAVAR KLERMAEQAM TSMYKQARAE DKKAKIVSAM QTMLFGMIKK LDNDVLNGVI 

      4090       4100       4110       4120       4130       4140 
ANARNGCVPL SIVPLCASNK LRVVIPDISV WNKVVNWPSV SYAGSLWDIT VINNVDNEVV 

      4150       4160       4170       4180       4190       4200 
KPTDVVETNE SLTWPLVIEC SRSSSSAVKL QNNEIHPKGL KTMVITAGVD QVNCNSSAVA 

      4210       4220       4230       4240       4250       4260 
YYEPVQGHRM VMGLLSENAH LKWAKVEGKD GFINIELQPP CKFLIAGPKG PEIRYLYFVK 

      4270       4280       4290       4300       4310       4320 
NLNNLHRGQL LGHIAATVRL QAGANTEFAS NSTVLTLVAF AVDPAKAYLD YVGSGGTPLS 

      4330       4340       4350       4360       4370       4380 
NYVKMLAPKT GTGVAISVKP EATADQETYG GASVCLYCRA HIEHPDVSGV CKYKTRFVQI 

      4390       4400       4410       4420       4430       4440 
PAHVRDPVGF LLKNVPCNVC QYWVGYGCNC DALRNNTVPQ SKDTNFLNRV RGSSVNARLE 

      4450       4460       4470       4480       4490       4500 
PCSSGLTTDV VYRAFDICNF KARVAGIGKY YKTNTCRFVQ VDDEGHKLDS YFIVKRHTMS 

      4510       4520       4530       4540       4550       4560 
NYELEKRCYD LLKDCDAVAI HDFFIFDVDK TKTPHIVRQS LTEYTMMDLV YALRHFDQNN 

      4570       4580       4590       4600       4610       4620 
CEVLKSILVK YGCCEQSYFD NKLWFDFVEN PSVIGVYHKL GERIRQAMLN TVKMCDHMVK 

      4630       4640       4650       4660       4670       4680 
SGLVGVLTLD NQDLNGKWYD FGDFVITQPG AGVAIVDSYY SYLMPVLSMT NCLAAETHKD 

      4690       4700       4710       4720       4730       4740 
CDFNKPLIEW PLLEYDYTDY KIGLFNKYFK YWDQTYHPNC VNCSDDRCIL HCANFNVLFS 

      4750       4760       4770       4780       4790       4800 
MVLPNTSFGP IVRKIFVDGV PFIVSCGYHY KELGLVMNMD FNIHRHRLAL KELMMYAADP 

      4810       4820       4830       4840       4850       4860 
AMHIASASAL WDLRTPCFSV AALTTGLTFQ TVRPGNFNKD FYDFVVSRGF FKEGSSVTLK 

      4870       4880       4890       4900       4910       4920 
HFFFAQDGHA AITDYSYYAY NLPTMVDIKQ MLFCMEVVDK YFDIYDGGCL NASEVIVNNL 

      4930       4940       4950       4960       4970       4980 
DKSAGHPFNK FGKARVYYES MSYQEQDELF AVTKRNVLPT ITQMNLKYAI SAKNRARTVA 

      4990       5000       5010       5020       5030       5040 
GVSILSTMTN RQYHQKMLKS MAATRGATCV IGTTKFYGGW DFMLKTLYKD VESPHLMGWD 

      5050       5060       5070       5080       5090       5100 
YPKCDRAMPN MCRILASLIL ARKHSTCCTN SDRFYRLANE CAQVLSEYVL CGGGYYVKPG 

      5110       5120       5130       5140       5150       5160 
GTSSGDATTA YANSVFNILQ ATTANVSALM SANGNTIIDR EIKDMQFDLY INVYRKVVPD 

      5170       5180       5190       5200       5210       5220 
PKFVDKYYAF LNKHFSMMIL SDDGVVCYNS DYAAKGYVAS IQNFKETLYY QNNVFMSEAK 

      5230       5240       5250       5260       5270       5280 
CWVETNLEKG PHEFCSQHTL YIKDGDDGYF LPYPDPSRIL SAGCFVDDIV KTDGTVMMER 

      5290       5300       5310       5320       5330       5340 
YVSLAIDAYP LTKHDDTEYQ NVFWVYLQYI EKLYKDLTGH MLDSYSVMLC GDDSAKFWEE 

      5350       5360       5370       5380       5390       5400 
GFYRDLYSSP TTLQAVGSCV VCHSQTSLRC GTCIRRPFLC CKCCYDHVIA TPHKMVLSVS 

      5410       5420       5430       5440       5450       5460 
PYVCNAPGCD VSDVTKLYLG GMSYYCNDHR PVCSFPLCAN GLVFGLYKNM CTGSSSIMEF 

      5470       5480       5490       5500       5510       5520 
NRLATCDWSD SGDYTLANTT TEPLKLFAAE TLRATEEASK QSYAIATIKE IVGERELILV 

      5530       5540       5550       5560       5570       5580 
WEVGKSKPPL NRNYVFTGYH LTKNSKVQLG EYVFERIDYS DAVSYKSSTT YKLAVGDIFV 

      5590       5600       5610       5620       5630       5640 
LTSHSVATLS APTIVNQERY LKITGIYPTI TVPEEFANHV VNFQKAGFSK YVTVQGPPGT 

      5650       5660       5670       5680       5690       5700 
GKSHFAIGLA IYYPTARIVY TACSHAAVDA LCEKAFKYLN IAKCSRIIPA KARVECYDRF 

      5710       5720       5730       5740       5750       5760 
KVNDTNSQYL FSTVNALPEI SVDILVVDEV SMCTNYDLSI INSRVKAKHI VYVGDPAQLP 

      5770       5780       5790       5800       5810       5820 
APRTLLIRGT LEPENFNSVT RLMCNLGPDI FLSVCYRCPK EIVSTVSALV YNNKLSAKKD 

      5830       5840       5850       5860       5870       5880 
ASGQCFKILF KGSVTHDASS AINRPQLNFV KTFIAANPNW SKAVFISPYN SQNAVARSML 

      5890       5900       5910       5920       5930       5940 
GLTTQTVDSS QGSEYPYVIF CQTADTAHAN NLNRFNVAVT RAQKGILCVM TSQVLFDSLE 

      5950       5960       5970       5980       5990       6000 
FAELSLNNYK LQSQIVTGLF KDCSREDTGL PPAYAPTYLS VDAKYKTTDE LCVNLNITPN 

      6010       6020       6030       6040       6050       6060 
VTYSRVISRM GFKLDATIPG YPKLFITRDE AIRQVRSWVG FDVEGAHASR NACGTNVPLQ 

      6070       6080       6090       6100       6110       6120 
LGFSTGVNFV VQPVGVVDTE WGSMLTTISA RPPPGEQFKH LVPLMNKGAT WPIVRRRIVQ 

      6130       6140       6150       6160       6170       6180 
MLSDTLDKLS DYCTFVCWAH GFELTSASYF CKIGKEQRCS MCSRRASTFS SPLQSYACWS 

      6190       6200       6210       6220       6230       6240 
HSSGYDYVYN PFFVDVQQWG YVGNLATNHD RYCGIHAGAH VASSDAIMTR CLAIYDCFIE 

      6250       6260       6270       6280       6290       6300 
RVDWDVTYPY ISHEQKLNSC CRTVERNVVR SAVLSGKFEK IYDIGNPKGI AIISEPVEWH 

      6310       6320       6330       6340       6350       6360 
FYDAQPLSNK VKKLFYTDDV SKQFEDGLCL FWNCNVSKYP SNAVVCRFDT RVHSEFNLPG 

      6370       6380       6390       6400       6410       6420 
CNGGSLYVNK HAFHTPAYDI NAFRDLKPLP FFYYSTTPCE VHGSGNMLED IDYVPLKSAV 

      6430       6440       6450       6460       6470       6480 
CITACNLGGA VCRKHAAEYR DYMEAYNIVS AAGFRLWVYK TFDIYNLWST FVKVQGLENI 

      6490       6500       6510       6520       6530       6540 
AFNVIKQGHF TGVDGELPVA VVNDKIFTKN GTDDVCIFKN ETALPTNVAF ELYAKRAVRS 

      6550       6560       6570       6580       6590       6600 
HPDLNLLRNL EVDVCYNFVL WDYDRNNIYG TTTIGVCKYT DIDVNPNLNM CFDIRDKGSL 

      6610       6620       6630       6640       6650       6660 
ERFMSMPNGV LISDRKIKNY PCISGPKHAY FNGAILRNID AKQPVIFYLY KKVNNEFVSF 

      6670       6680       6690       6700       6710       6720 
SDTFYTCGRT VGDFTVLTPM EEDFLVLDSD VFIKKYGLED YAFEHVVYGD FSHTTLGGLH 

      6730       6740       6750       6760       6770       6780 
LLIGLYKKMR EGHILMEEML KDRATVHNYF ITDSNTASYK AVCSVIDLRL DDFVTIIKEM 

      6790       6800       6810       6820       6830       6840 
DLDVVSKVVK VPIDLTMIEF MLWCRDGKVQ TFYPRLQATN DWKPGLTMPS LFKVQQMNLE 

      6850       6860       6870       6880       6890       6900 
PCLLANYKQS IPMPNGVHMN VAKYMQLCQY LNTCTLAVPA NMRVIHFGAG CEKGVAPGTS 

      6910       6920       6930       6940       6950       6960 
VLRQWLPLDA VLIDNDLNEF VSDADITIFG DCVTVHVGQQ VDLLISDMYD PCTKAVGEVN 

      6970       6980       6990       7000       7010       7020 
QTKALFFVYL CNFIKNNLAL GGSVAIKITE HSWSADLYKI MGRFAYWTVF CTNANASSSE 

      7030       7040       7050       7060       7070       7080 
GFLIGINFLG ELKEEIDGNV MHANYIFWRN STPMNLSTYS LFDLSRFPLK LKGTPVLQLK 

      7090       7100       7110 
ESQINELVIS LLSQGKLLIR DNDTLNVSTD VLVNFRKRL 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6T4.

References

[1]"Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features."
Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y.
J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate HKU4-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF065505 Genomic RNA. Translation: ABN10838.1.
RefSeqYP_001039952.1. NC_009019.1. [P0C6W3-1]

3D structure databases

ProteinModelPortalP0C6W3.
SMRP0C6W3. Positions 3889-3972, 4221-4281, 4291-4411.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0C6W3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4835998.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_BCHK4
AccessionPrimary (citable) accession number: P0C6W3
Secondary accession number(s): A3EX93
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries