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P0C6W3

- R1AB_BCHK4

UniProt

P0C6W3 - R1AB_BCHK4

Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity
    The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.By similarity
    The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
    Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    ATP + H2O = ADP + phosphate.
    TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei195 – 1962CleavageSequence Analysis
    Sitei847 – 8482Cleavage; by PL-PROSequence Analysis
    Active sitei1634 – 16341For PL-PRO activityPROSITE-ProRule annotation
    Active sitei1800 – 18001For PL-PRO activityPROSITE-ProRule annotation
    Sitei2784 – 27852Cleavage; by PL-PROSequence Analysis
    Sitei3291 – 32922Cleavage; by 3CL-PROSequence Analysis
    Active sitei3332 – 33321For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3439 – 34391For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3597 – 35982Cleavage; by 3CL-PROSequence Analysis
    Sitei3889 – 38902Cleavage; by 3CL-PROSequence Analysis
    Sitei3972 – 39732Cleavage; by 3CL-PROSequence Analysis
    Sitei4171 – 41722Cleavage; by 3CL-PROSequence Analysis
    Sitei4281 – 42822Cleavage; by 3CL-PROSequence Analysis
    Sitei4420 – 44212Cleavage; by 3CL-PROSequence Analysis
    Sitei5354 – 53552Cleavage; by 3CL-PROSequence Analysis
    Sitei5952 – 59532Cleavage; by 3CL-PROSequence Analysis
    Sitei6475 – 64762Cleavage; by 3CL-PROSequence Analysis
    Sitei6817 – 68182Cleavage; by 3CL-PROSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1714 – 175138C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4355 – 437117By similarityAdd
    BLAST
    Zinc fingeri4397 – 441014By similarityAdd
    BLAST
    Nucleotide bindingi5636 – 56438ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. helicase activity Source: UniProtKB-KW
    6. methyltransferase activity Source: InterPro
    7. omega peptidase activity Source: InterPro
    8. RNA binding Source: UniProtKB-KW
    9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    10. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    2. induction by virus of host autophagy Source: UniProtKB-KW
    3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    4. suppression by virus of host gene expression Source: UniProtKB-KW
    5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    7. transcription, DNA-templated Source: InterPro
    8. viral protein processing Source: InterPro
    9. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Short name:
    pp1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 15 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    Leader protein
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p65 homolog
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name:
    PL-PRO
    Non-structural protein 4
    Short name:
    nsp4
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    nsp5
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Non-structural protein 8
    Short name:
    nsp8
    Non-structural protein 9
    Short name:
    nsp9
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    nsp12
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    nsp13
    Exoribonuclease (EC:3.1.13.-)
    Short name:
    ExoN
    Alternative name(s):
    nsp14
    Alternative name(s):
    NendoU
    nsp15
    Alternative name(s):
    nsp16
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiBat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004)
    Taxonomic identifieri694007 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiTylonycteris pachypus (Lesser bamboo bat) [TaxID: 258959]
    ProteomesiUP000006574: Genome

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
    Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. host cell membrane Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Non-structural protein 1Sequence AnalysisPRO_0000290288Add
    BLAST
    Chaini196 – 847652Non-structural protein 2Sequence AnalysisPRO_0000290289Add
    BLAST
    Chaini848 – 27841937Non-structural protein 3Sequence AnalysisPRO_0000290290Add
    BLAST
    Chaini2785 – 3291507Non-structural protein 4Sequence AnalysisPRO_0000290291Add
    BLAST
    Chaini3292 – 35973063C-like proteinaseSequence AnalysisPRO_0000290292Add
    BLAST
    Chaini3598 – 3889292Non-structural protein 6Sequence AnalysisPRO_0000290293Add
    BLAST
    Chaini3890 – 397283Non-structural protein 7Sequence AnalysisPRO_0000290294Add
    BLAST
    Chaini3973 – 4171199Non-structural protein 8Sequence AnalysisPRO_0000290295Add
    BLAST
    Chaini4172 – 4281110Non-structural protein 9Sequence AnalysisPRO_0000290296Add
    BLAST
    Chaini4282 – 4420139Non-structural protein 10Sequence AnalysisPRO_0000290297Add
    BLAST
    Chaini4421 – 5354934RNA-directed RNA polymeraseSequence AnalysisPRO_0000290298Add
    BLAST
    Chaini5355 – 5952598HelicaseSequence AnalysisPRO_0000290299Add
    BLAST
    Chaini5953 – 6475523ExoribonucleaseSequence AnalysisPRO_0000290300Add
    BLAST
    Chaini6476 – 6817342Uridylate-specific endoribonucleaseSequence AnalysisPRO_0000290301Add
    BLAST
    Chaini6818 – 7119302Putative 2'-O-methyl transferaseSequence AnalysisPRO_0000290302Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Proteomic databases

    PRIDEiP0C6W3.

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C6W3.
    SMRiP0C6W3. Positions 3889-3972, 4221-4281, 4291-4411.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2112 – 213221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2145 – 216521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2222 – 224221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2326 – 234621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2350 – 237021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2375 – 239521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2800 – 282021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3072 – 309221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3105 – 312521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3149 – 316921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3603 – 362321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3637 – 365721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3662 – 368221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3707 – 372721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3735 – 375521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3784 – 380421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3808 – 382821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1152 – 1321170MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1593 – 1864272Peptidase C16PROSITE-ProRule annotationAdd
    BLAST
    Domaini3292 – 3597306Peptidase C30PROSITE-ProRule annotationAdd
    BLAST
    Domaini5034 – 5196163RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini5355 – 543884CV MBDAdd
    BLAST
    Domaini5611 – 5792182(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini5793 – 5967175(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2112 – 2395284HD1By similarityAdd
    BLAST
    Regioni2800 – 3169370HD2By similarityAdd
    BLAST
    Regioni3603 – 3828226HD3By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi960 – 104990Glu-richAdd
    BLAST
    Compositional biasi4161 – 41666Poly-Ser
    Compositional biasi5359 – 538426Cys-richAdd
    BLAST

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1714 – 175138C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4355 – 437117By similarityAdd
    BLAST
    Zinc fingeri4397 – 441014By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SMARTiSM00506. A1pp. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1ab (identifier: P0C6W3-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MLSKASVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTDD MACKLTPWFE     50
    DGETAFNQVS SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS 100
    KHFLVNQLAY KDQVGAAMMR TTLNAKPLGM FFPYDSSLET GEYTFLLRKN 150
    GLGGQLFRER PWDRKETPYV EILDDLEADP TGKYSQNLLK KLIGGDCIPI 200
    DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS DRFIVLNKKL 250
    YRVVWNVTRR NVPYPKQTAF TIVSVVQCDD KDSVPEHTFT IGSQILMVSP 300
    LKATNNKNFN LKQRLLYTFY GKDAVQQPGY IYHSAYVDCN ACGRGTWCTG 350
    NAIQGFACDC GANYSANDVD LQSSGLVPRN ALFLANCPCA NNGACSHSAA 400
    QVYNILDGKA CVEVGGKSFT LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR 450
    IGDAIFTGCT GTWDKVVETA NLFLEKAQRS LNFCQQFALT EVVLAILSGT 500
    TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI NIGANGVCNA 550
    TINAPFIAFT GLGESFKKVS AIPWKICSNL KSALDYYSSN IMFRVFPYDI 600
    PCDVSNFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SSACQTALSS 650
    FLNACVAASR ATAGFINDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ 700
    QVLDIMSKAM KLLHTNVSWA GTKLSAIIYE GREALLFNSG TYFCLSTKAK 750
    TLQGQMNLVL PGDYNKKTLG ILDPVPNADT IDVNANSTVV DVVHGQLEPT 800
    NEHGPSMIVG NYVLVSDKLF VRTEDEEFYP LCTNGKVVST LFRLKGGMPS 850
    KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE 900
    DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN 950
    MTFSINPVED VEEVEEFIED DYLSDELPIA DDEEAWARAV EEVMPLDDIL 1000
    VAEIELEEDP PLETALESVE AEVVETAEAQ EPSVESIDST PSTSTVVGEN 1050
    DLSVKPMSRV AETDDVLELE TAVVGGPVSD VTAIVTNDIV SVEQAQQCGV 1100
    SSLPIQDEAS ENQVHQVSDL QGNELLCSET KVEIVQPRQD LKPRRSRKSK 1150
    VDLSKYKHTV INNSVTLVLG DAIQIASLLP KCILVNAANR HLKHGGGIAG 1200
    VINKASGGDV QEESDEYISN NGPLHVGDSV LLKGHGLADA ILHVVGPDAR 1250
    NNEDAALLKR CYKAFNKHTI VVTPLISAGI FSVDPKVSFE YLLANVTTTT 1300
    YVVVNNEDIY NTLATPSKPD GLVYSFEGWR GTVRTAKNYG FTCFICTEYS 1350
    ANVKFLRTKG VDTTKKIQTV DGVSYYLYSA RDALTDVIAA ANGCSGICAM 1400
    PFGYVTHGLD LAQSGNYVRQ VKVPYVCLLA SKEQIPIMNS DVAIQTPETA 1450
    FINNVTSNGG YHSWHLVSGD LIVKDVCYKK LLHWSGQTIC YADNKFYVVK 1500
    NDVALPFSDL EACRAYLTSR AAQQVNIEVL VTIDGVNFRT VILNDTTTFR 1550
    KQLGATFYKG VDISDAFPTV KMGGESLFVA DNLSESEKVV LKEYYGTSDV 1600
    TFLQRYYSLQ PLVQQWKFVV HDGVKSLKLS NYNCYINATI MMIDMLHDIK 1650
    FVVPALQNAY LRYKGGDPYD FLALIMAYGD CTFDNPDDEA KLLHTLLAKA 1700
    ELTVSAKMVW REWCTVCGIR DIEYTGMRAC VYAGVNSMEE LQSVFNETCV 1750
    CGSVKHRQLV EHSAPWLLVS GLNEVKVSTS TDPIYRAFNV FQGVETSVGH 1800
    YVHIRVKDGL FYKYDSGSLT KTSDMKCKMT SVWYPTVRYT ADCNVVVYDL 1850
    DGVTKVEVNP DLSNYYMKDG KYYTSKPTIK YSPATILPGS VYSNSCLVGV 1900
    DGTPGSDTIS KFFNDLLGFD ETKPISKKLT YSLLPNEDGD VLLSEFSNYN 1950
    PVYKKGVMLK GKPILWVNNG VCDSALNKPN RASLRQLYDV APIVLDNKYT 2000
    VLQDNTSQLV EHNVPVVDDV PITTRKLIEV KCKGLNKPFV KGNFSFVNDP 2050
    NGVTVVDTLG LTELRALYVD INTRYIVLRD NNWSSLFKLH TVESGDLQIV 2100
    AAGGSVTRRA RVLLGASSLF ASFAKITVTA TTAACKTAGR GFCKFVVNYG 2150
    VLQNMFVFLK MLFFLPFNYL WPKKQPTVDI GVSGLRTAGI VTTNIVKQCG 2200
    TAAYYMLLGK FKRVDWKATL RLFLLLCTTI LLLSSIYHLV LFNQVLSSDV 2250
    MLEDATGILA IYKEVRSYLG IRTLCDGLVV EYRNTSFDVM EFCSNRSVLC 2300
    QWCLIGQDSL TRYSALQMLQ THITSYVLNI DWIWFALEFF LAYVLYTSSF 2350
    NVLLLVVTAQ YFFAYTSAFV NWRAYNYIVS GLFFLVTHIP LHGLVRVYNF 2400
    LACLWFLRKF YSHVINGCKD TACLLCYKRN RLTRVEASTI VCGTKRTFYI 2450
    AANGGTSYCC KHNWNCVECD TAGVGNTFIC TEVANDLTTT LRRLIKPTDQ 2500
    SHYYVDSVVV KDAVVELHYN RDGSSCYERY PLCYFTNLEK LKFKEVCKTP 2550
    TGIPEHNFLI YDTNDRGQEN LARSACVYYS QVLCKPMLLV DVNLVTTVGD 2600
    SREIAIKMLD SFINSFISLF SVSRDKLEKL INTARDCVRR GDDFQNVLKT 2650
    FTDAARGHAG VESDVETTMV VDALQYAHKN DIQLTTECYN NYVPGYIKPD 2700
    SINTLDLGCL IDLKAASVNQ TSMRNANGAC VWNSGDYMKL SDSFKRQIRI 2750
    ACRKCNIPFR LTTSKLRAAD NILSVKFSAT KIVGGAPSWL LRVRDLTVKG 2800
    YCILTLFVFT VAVLSWFCLP SYSIATVNFN DDRILTYKVI ENGIVRDIAP 2850
    NDVCFANKYG HFSKWFNENH GGVYRNSMDC PITIAVIAGV AGARVANVPA 2900
    NLAWVGKQIV LFVSRVFANT NVCFTPINEI PYDTFSDSGC VLSSECTLFR 2950
    DAEGNLNPFC YDPTVLPGAS SYADMKPHVR YDMYDSDMYI KFPEVIVEST 3000
    LRITKTLATQ YCRFGSCEES AAGVCISTNG SWALYNQNYS TRPGIYCGDD 3050
    YFDIVRRLAI SLFQPVTYFQ LSTSLAMGLV LCVFLTAAFY YINKVKRALA 3100
    DYTQCAVVAV VAALLNSLCL CFIVANPLLV APYTAMYYYA TFYLTGEPAF 3150
    IMHISWYVMF GAVVPIWMLA SYTVGVMLRH LFWVLAYFSK KHVDVFTDGK 3200
    LNCSFQDAAS NIFVIGKDTY VALRNAITQD SFVRYLSLFN KYKYYSGAMD 3250
    TASYREACAA HLCKALQTYS ETGSDILYQP PNCSVTSSVL QSGLVKMSAP 3300
    SGAVENCIVQ VTCGSMTLNG LWLDNTVWCP RHIMCPADQL TDPNYDALLI 3350
    SKTNHSFIVQ KHIGAQANLR VVAHSMVGVL LKLTVDVANP STPAYTFSTV 3400
    KPGASFSVLA CYNGKPTGVF TVNLRHNSTI KGSFLCGSCG SVGYTENGGV 3450
    INFVYMHQME LSNGTHTGSS FDGVMYGAFE DKQTHQLQLT DKYCTINVVA 3500
    WLYAAVLNGC KWFVKPTRVG IVTYNEWALS NQFTEFVGTQ SIDMLAHRTG 3550
    VSVEQMLAAI QSLHAGFQGK TILGQSTLED EFTPDDVNMQ VMGVVMQSGV 3600
    KRISYGFIHW LISTFVLAYV SVMQLTKFTM WTYLFETIPT QMTPLLLGFM 3650
    ACVMFTVKHK HTFMSLFLLP VALCLTYANI VYEPQTLISS TLIAVANWLT 3700
    PTSVYMRTTH FDFGLYISLS FVLAIIVRRL YRPSMSNLAL ALCSGVMWFY 3750
    TYVIGDHSSP ITYLMFITTL TSDYTITVFA TVNLAKFISG LVFFYAPHLG 3800
    FILPEVKLVL LIYLGLGYMC TMYFGVFSLL NLKLRVPLGV YDYSVSTQEF 3850
    RFLTGNGLHA PRNSWEALIL NFKLLGIGGT PCIKVATVQS KLTDLKCTSV 3900
    VLLTVLQQLH LESNSKAWSY CVKLHNEILA AVDPTEAFER FVCLFATLMS 3950
    FSANVDLDAL ANDLFENSSV LQATLTEFSH LATYAELETA QSSYQKALNS 4000
    GDASPQVLKA LQKAVNVAKN AYEKDKAVAR KLERMAEQAM TSMYKQARAE 4050
    DKKAKIVSAM QTMLFGMIKK LDNDVLNGVI ANARNGCVPL SIVPLCASNK 4100
    LRVVIPDISV WNKVVNWPSV SYAGSLWDIT VINNVDNEVV KPTDVVETNE 4150
    SLTWPLVIEC SRSSSSAVKL QNNEIHPKGL KTMVITAGVD QVNCNSSAVA 4200
    YYEPVQGHRM VMGLLSENAH LKWAKVEGKD GFINIELQPP CKFLIAGPKG 4250
    PEIRYLYFVK NLNNLHRGQL LGHIAATVRL QAGANTEFAS NSTVLTLVAF 4300
    AVDPAKAYLD YVGSGGTPLS NYVKMLAPKT GTGVAISVKP EATADQETYG 4350
    GASVCLYCRA HIEHPDVSGV CKYKTRFVQI PAHVRDPVGF LLKNVPCNVC 4400
    QYWVGYGCNC DALRNNTVPQ SKDTNFLNRV RGSSVNARLE PCSSGLTTDV 4450
    VYRAFDICNF KARVAGIGKY YKTNTCRFVQ VDDEGHKLDS YFIVKRHTMS 4500
    NYELEKRCYD LLKDCDAVAI HDFFIFDVDK TKTPHIVRQS LTEYTMMDLV 4550
    YALRHFDQNN CEVLKSILVK YGCCEQSYFD NKLWFDFVEN PSVIGVYHKL 4600
    GERIRQAMLN TVKMCDHMVK SGLVGVLTLD NQDLNGKWYD FGDFVITQPG 4650
    AGVAIVDSYY SYLMPVLSMT NCLAAETHKD CDFNKPLIEW PLLEYDYTDY 4700
    KIGLFNKYFK YWDQTYHPNC VNCSDDRCIL HCANFNVLFS MVLPNTSFGP 4750
    IVRKIFVDGV PFIVSCGYHY KELGLVMNMD FNIHRHRLAL KELMMYAADP 4800
    AMHIASASAL WDLRTPCFSV AALTTGLTFQ TVRPGNFNKD FYDFVVSRGF 4850
    FKEGSSVTLK HFFFAQDGHA AITDYSYYAY NLPTMVDIKQ MLFCMEVVDK 4900
    YFDIYDGGCL NASEVIVNNL DKSAGHPFNK FGKARVYYES MSYQEQDELF 4950
    AVTKRNVLPT ITQMNLKYAI SAKNRARTVA GVSILSTMTN RQYHQKMLKS 5000
    MAATRGATCV IGTTKFYGGW DFMLKTLYKD VESPHLMGWD YPKCDRAMPN 5050
    MCRILASLIL ARKHSTCCTN SDRFYRLANE CAQVLSEYVL CGGGYYVKPG 5100
    GTSSGDATTA YANSVFNILQ ATTANVSALM SANGNTIIDR EIKDMQFDLY 5150
    INVYRKVVPD PKFVDKYYAF LNKHFSMMIL SDDGVVCYNS DYAAKGYVAS 5200
    IQNFKETLYY QNNVFMSEAK CWVETNLEKG PHEFCSQHTL YIKDGDDGYF 5250
    LPYPDPSRIL SAGCFVDDIV KTDGTVMMER YVSLAIDAYP LTKHDDTEYQ 5300
    NVFWVYLQYI EKLYKDLTGH MLDSYSVMLC GDDSAKFWEE GFYRDLYSSP 5350
    TTLQAVGSCV VCHSQTSLRC GTCIRRPFLC CKCCYDHVIA TPHKMVLSVS 5400
    PYVCNAPGCD VSDVTKLYLG GMSYYCNDHR PVCSFPLCAN GLVFGLYKNM 5450
    CTGSSSIMEF NRLATCDWSD SGDYTLANTT TEPLKLFAAE TLRATEEASK 5500
    QSYAIATIKE IVGERELILV WEVGKSKPPL NRNYVFTGYH LTKNSKVQLG 5550
    EYVFERIDYS DAVSYKSSTT YKLAVGDIFV LTSHSVATLS APTIVNQERY 5600
    LKITGIYPTI TVPEEFANHV VNFQKAGFSK YVTVQGPPGT GKSHFAIGLA 5650
    IYYPTARIVY TACSHAAVDA LCEKAFKYLN IAKCSRIIPA KARVECYDRF 5700
    KVNDTNSQYL FSTVNALPEI SVDILVVDEV SMCTNYDLSI INSRVKAKHI 5750
    VYVGDPAQLP APRTLLIRGT LEPENFNSVT RLMCNLGPDI FLSVCYRCPK 5800
    EIVSTVSALV YNNKLSAKKD ASGQCFKILF KGSVTHDASS AINRPQLNFV 5850
    KTFIAANPNW SKAVFISPYN SQNAVARSML GLTTQTVDSS QGSEYPYVIF 5900
    CQTADTAHAN NLNRFNVAVT RAQKGILCVM TSQVLFDSLE FAELSLNNYK 5950
    LQSQIVTGLF KDCSREDTGL PPAYAPTYLS VDAKYKTTDE LCVNLNITPN 6000
    VTYSRVISRM GFKLDATIPG YPKLFITRDE AIRQVRSWVG FDVEGAHASR 6050
    NACGTNVPLQ LGFSTGVNFV VQPVGVVDTE WGSMLTTISA RPPPGEQFKH 6100
    LVPLMNKGAT WPIVRRRIVQ MLSDTLDKLS DYCTFVCWAH GFELTSASYF 6150
    CKIGKEQRCS MCSRRASTFS SPLQSYACWS HSSGYDYVYN PFFVDVQQWG 6200
    YVGNLATNHD RYCGIHAGAH VASSDAIMTR CLAIYDCFIE RVDWDVTYPY 6250
    ISHEQKLNSC CRTVERNVVR SAVLSGKFEK IYDIGNPKGI AIISEPVEWH 6300
    FYDAQPLSNK VKKLFYTDDV SKQFEDGLCL FWNCNVSKYP SNAVVCRFDT 6350
    RVHSEFNLPG CNGGSLYVNK HAFHTPAYDI NAFRDLKPLP FFYYSTTPCE 6400
    VHGSGNMLED IDYVPLKSAV CITACNLGGA VCRKHAAEYR DYMEAYNIVS 6450
    AAGFRLWVYK TFDIYNLWST FVKVQGLENI AFNVIKQGHF TGVDGELPVA 6500
    VVNDKIFTKN GTDDVCIFKN ETALPTNVAF ELYAKRAVRS HPDLNLLRNL 6550
    EVDVCYNFVL WDYDRNNIYG TTTIGVCKYT DIDVNPNLNM CFDIRDKGSL 6600
    ERFMSMPNGV LISDRKIKNY PCISGPKHAY FNGAILRNID AKQPVIFYLY 6650
    KKVNNEFVSF SDTFYTCGRT VGDFTVLTPM EEDFLVLDSD VFIKKYGLED 6700
    YAFEHVVYGD FSHTTLGGLH LLIGLYKKMR EGHILMEEML KDRATVHNYF 6750
    ITDSNTASYK AVCSVIDLRL DDFVTIIKEM DLDVVSKVVK VPIDLTMIEF 6800
    MLWCRDGKVQ TFYPRLQATN DWKPGLTMPS LFKVQQMNLE PCLLANYKQS 6850
    IPMPNGVHMN VAKYMQLCQY LNTCTLAVPA NMRVIHFGAG CEKGVAPGTS 6900
    VLRQWLPLDA VLIDNDLNEF VSDADITIFG DCVTVHVGQQ VDLLISDMYD 6950
    PCTKAVGEVN QTKALFFVYL CNFIKNNLAL GGSVAIKITE HSWSADLYKI 7000
    MGRFAYWTVF CTNANASSSE GFLIGINFLG ELKEEIDGNV MHANYIFWRN 7050
    STPMNLSTYS LFDLSRFPLK LKGTPVLQLK ESQINELVIS LLSQGKLLIR 7100
    DNDTLNVSTD VLVNFRKRL 7119

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:7,119
    Mass (Da):795,200
    Last modified:June 10, 2008 - v1
    Checksum:iE85475854586DD79
    GO
    Isoform Replicase polyprotein 1a (identifier: P0C6T4-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform can be found in the external entry P0C6T4.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by conventional translation.

    Length:4,434
    Mass (Da):491,823
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF065505 Genomic RNA. Translation: ABN10838.1.
    RefSeqiYP_001039952.1. NC_009019.1. [P0C6W3-1]

    Genome annotation databases

    GeneIDi4835998.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF065505 Genomic RNA. Translation: ABN10838.1 .
    RefSeqi YP_001039952.1. NC_009019.1. [P0C6W3-1 ]

    3D structure databases

    ProteinModelPortali P0C6W3.
    SMRi P0C6W3. Positions 3889-3972, 4221-4281, 4291-4411.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0C6W3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4835998.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF06478. Corona_RPol_N. 1 hit.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view ]
    SMARTi SM00506. A1pp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEi PS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features."
      Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y.
      J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate HKU4-1.

    Entry informationi

    Entry nameiR1AB_BCHK4
    AccessioniPrimary (citable) accession number: P0C6W3
    Secondary accession number(s): A3EX93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3