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P0C6W2

- R1AB_BCHK3

UniProt

P0C6W2 - R1AB_BCHK3

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Protein
Replicase polyprotein 1ab
Gene
rep, 1a-1b
Organism
Bat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.
Nsp9 is a ssRNA-binding protein By similarity.
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1802Cleavage; by PL-PRO By similarity
Sitei818 – 8192Cleavage; by PL-PRO By similarity
Active sitei1645 – 16451For PL-PRO activity By similarity
Active sitei1806 – 18061For PL-PRO activity By similarity
Sitei3234 – 32352Cleavage; by 3CL-PRO By similarity
Active sitei3275 – 32751For 3CL-PRO activity By similarity
Active sitei3379 – 33791For 3CL-PRO activity By similarity
Sitei3540 – 35412Cleavage; by 3CL-PRO By similarity
Sitei3830 – 38312Cleavage; by 3CL-PRO By similarity
Sitei3913 – 39142Cleavage; by 3CL-PRO By similarity
Sitei4111 – 41122Cleavage; by 3CL-PRO By similarity
Sitei4224 – 42252Cleavage; by 3CL-PRO By similarity
Sitei4363 – 43642Cleavage; by 3CL-PRO By similarity
Sitei5295 – 52962Cleavage; by 3CL-PRO By similarity
Sitei5896 – 58972Cleavage; by 3CL-PRO By similarity
Sitei6423 – 64242Cleavage; by 3CL-PRO By similarity
Sitei6769 – 67702Cleavage; by 3CL-PRO By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1723 – 176038C4-type By similarity
Add
BLAST
Zinc fingeri4298 – 431417 By similarity
Add
BLAST
Zinc fingeri4341 – 435414 By similarity
Add
BLAST
Nucleotide bindingi5577 – 55848ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. endonuclease activity Source: UniProtKB-KW
  6. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  7. helicase activity Source: UniProtKB-KW
  8. methyltransferase activity Source: InterPro
  9. omega peptidase activity Source: InterPro
  10. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  5. suppression by virus of host translation Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral RNA genome replication Source: InterPro
  9. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Protein family/group databases

MEROPSiC16.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3)
Taxonomic identifieri442736 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399]
ProteomesiUP000007450: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2197 – 221721Helical; Reviewed prediction
Add
BLAST
Transmembranei2298 – 231821Helical; Reviewed prediction
Add
BLAST
Transmembranei2345 – 236521Helical; Reviewed prediction
Add
BLAST
Transmembranei2744 – 276421Helical; Reviewed prediction
Add
BLAST
Transmembranei2986 – 300621Helical; Reviewed prediction
Add
BLAST
Transmembranei3016 – 303621Helical; Reviewed prediction
Add
BLAST
Transmembranei3048 – 306821Helical; Reviewed prediction
Add
BLAST
Transmembranei3071 – 309121Helical; Reviewed prediction
Add
BLAST
Transmembranei3099 – 311921Helical; Reviewed prediction
Add
BLAST
Transmembranei3136 – 315621Helical; Reviewed prediction
Add
BLAST
Transmembranei3558 – 357821Helical; Reviewed prediction
Add
BLAST
Transmembranei3580 – 360021Helical; Reviewed prediction
Add
BLAST
Transmembranei3606 – 362621Helical; Reviewed prediction
Add
BLAST
Transmembranei3652 – 367221Helical; Reviewed prediction
Add
BLAST
Transmembranei3679 – 369820Helical; Reviewed prediction
Add
BLAST
Transmembranei3722 – 374221Helical; Reviewed prediction
Add
BLAST
Transmembranei3750 – 377021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Non-structural protein 1 By similarity
PRO_0000291335Add
BLAST
Chaini180 – 818639Non-structural protein 2 By similarity
PRO_0000291336Add
BLAST
Chaini819 – 27341916Non-structural protein 3 By similarity
PRO_0000291337Add
BLAST
Chaini2735 – 3234500Non-structural protein 4 By similarity
PRO_0000291338Add
BLAST
Chaini3235 – 35403063C-like proteinase By similarity
PRO_0000291339Add
BLAST
Chaini3541 – 3830290Non-structural protein 6 By similarity
PRO_0000291340Add
BLAST
Chaini3831 – 391383Non-structural protein 7 By similarity
PRO_0000291341Add
BLAST
Chaini3914 – 4111198Non-structural protein 8 By similarity
PRO_0000291342Add
BLAST
Chaini4112 – 4224113Non-structural protein 9 By similarity
PRO_0000291343Add
BLAST
Chaini4225 – 4363139Non-structural protein 10 By similarity
PRO_0000291344Add
BLAST
Chaini4364 – 5295932RNA-directed RNA polymerase By similarity
PRO_0000291345Add
BLAST
Chaini5296 – 5896601Helicase By similarity
PRO_0000291346Add
BLAST
Chaini5897 – 6423527Exoribonuclease By similarity
PRO_0000291347Add
BLAST
Chaini6424 – 6769346Uridylate-specific endoribonuclease By similarity
PRO_0000291348Add
BLAST
Chaini6770 – 7067298Putative 2'-O-methyl transferase By similarity
PRO_0000291349Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6W2.
SMRiP0C6W2. Positions 13-127, 819-930, 997-1171, 1325-1463, 1535-1846, 3235-3540, 3830-3913, 3915-4105, 4112-4224, 4234-4356, 6424-6767.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini998 – 1164167Macro
Add
BLAST
Domaini1605 – 1869265Peptidase C16
Add
BLAST
Domaini3235 – 3540306Peptidase C30
Add
BLAST
Domaini4975 – 5137163RdRp catalytic
Add
BLAST
Domaini5296 – 537984CV MBD
Add
BLAST
Domaini5552 – 5733182(+)RNA virus helicase ATP-binding
Add
BLAST
Domaini5734 – 5903170(+)RNA virus helicase C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2086 – 2365280HD1 By similarity
Add
BLAST
Regioni2749 – 3156408HD2 By similarity
Add
BLAST
Regioni3558 – 3770213HD3 By similarity
Add
BLAST
Regioni4906 – 5208303RNA-directed RNA polymerase By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi930 – 99667Glu-rich
Add
BLAST
Compositional biasi2204 – 22074Poly-Leu
Compositional biasi3760 – 37634Poly-Cys

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W2-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MESLVLGVNE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKNGT     50
CGLVELEKGV LPQLEQPYVF IKRSDALSTN HGHKVVELVA ELDGIQFGRS 100
GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG 150
TDPIEDYEQN WNTKHGSGAL RELTRELNGG VVTRYVDNNF CGPDGYPLEC 200
IKDFLARAGK SMCTLSEQLD YIESKRGVYC CREHEHEIVW FTERSEKSYE 250
HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI 300
RSVYPVATPQ ECNDMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV 350
CEGPTTCGYL PTNAVVKMPC PACQDPEVGP EHSVADYHNH SNIETRLRKG 400
GRTKCFGGCV FSYVGCYNKR AYWVPRASAN IGANHTGITG ENVETLNEDL 450
LEILNRERVN INIVGDFRFN EEVAIILASF SASPSAFIET VKGLDYKSFK 500
VIVESCGNYK VTNGKPVTGA WNIGQQRSIL TPLCGFPSQA AGVIRSIFSR 550
TLDAANHSIL DLQRAAVTTL DGISEQSLRL VDAMVYTSDL LTNSVVVMAY 600
VTGGLVQQTM QWLSNMLGTA VDKLKPVFTW VEAKLSAGVE FLRDAWEILK 650
FLITGVFDVI KGQIQVATDN IKECVKIFLG VVNKALEMCL DQVTIAGTKL 700
RALNLGEVFI AQSRGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDAHDTV 750
LTSEEVVLKS GELEALETPI DSFTSGAVVG TPVCINGLML LELENKEQYC 800
ALSPGLLATN NVFRLKGGAP VKGVTFGEDT VLEVQGYKNV KITFELDVRV 850
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTPMGIDLDE 900
WSVATFYLFD DAGEEKLSSR MYCSFYPPDE EEDCEECEDE EETCEHEYGT 950
EDDYKGLPLE FGASTETPHV EEEEEEEDWL DDAIEAEPEP EPLPEEPVNQ 1000
FVGYLKLTDN VAIKCIDIVK EAQSAKPTVI VNAANTHLKH GGGVAGALNK 1050
ATNGAMQNES DEYIRQNGPL TVGGSCLLSG HNLAEKCLHV VGPNLNAGED 1100
VQLLKRAYEN FNSQDVLLAP LLSAGIFGAK PLQSLKMCVE IVRTQVYLAV 1150
NDKSLYDQIV LDYLDSLKPK VESPNKEEEP KLEEPKAVQP VAEKPVDVKP 1200
KIKACIDEVT TTLEETKFLT NKLLLFADIN GKLYQDSQNM LRGEDMSFLE 1250
KDAPYIVGDV ITSGDITCVI IPAKKSGGTT EMLARALKEV PVAEYITTYP 1300
GQGCAGYTLE EAKTALKKCK SAFYVLPSET PNEKEEVLGT VSWNLREMLA 1350
HAEETRKLMP ICLDVRAIMA TIQRKYKGIK VQEGIVDYGV RFFFYTSKEP 1400
VASIITKLNS LNEPLVTMPI GYVTHGLNLE EAARCMRSLK APAVVSVSSP 1450
DAVTAYNGYL TSSSKTPEEY FVETTSLAGS YRDWSYSGQR TELGVEFLKR 1500
GDKIVYHTTG SPIEFHLDGE VLPLDKLKSL LSLREVKTIK VFTTVDNTNL 1550
HTHIVDMSMT YGQQFGPTYL DGADVTKIKP HVNHEGKTFF VLPSDDTLRS 1600
EAFEYYHTID ESFLGRYMSA LNHTKKWKFP QVGGLTSIKW ADNNCYLSSV 1650
LLALQQVEVK FNAPALQEAY YRARAGDAAN FCALILAYSN KTVGELGDVR 1700
ETMTHLLQHA NLESAKRVLN VVCKHCGQKT TTLKGVEAVM YMGTLSYDEL 1750
KTGVSIPCVC GRNATQYLVQ QESSFVMMSA PPAEYKLQQG AFLCANEYTG 1800
NYQCGHYTHI TAKETLYRVD GAHLTKMSEY KGPVTDVFYK ETSYTTAIKP 1850
VSYKLDGVTY TEIEPKLDGY YKKGNAYYTE QPIDLVPTQP MPNASFDNFK 1900
LTCSNTKFAD DLNQMTGFKK PASRELTVTF FPDLNGDVVA IDYRHYSTSF 1950
KKGAKLVHKP ILWHINQTTN KTTYKPNIWC LRCLWSTKPV DTSNSFEVLV 2000
VEDTQGMDNL ACESQTTTSE EVVENPTVQK EIIECDVKTT EVVGNVILKP 2050
SEEGVKVTQE LGHEDLMAAY VEETSITIKK PNELSLALGL KTLATHGAAA 2100
INSVPWSKIL AYVKPFLGQT AVITSNCIKK CVQRVFSNYM PYVITLLFQL 2150
CTFTKSTNSR IKASLPTTIA KNSVKSVAKL CLDVCINYVK SPKFSKLFTI 2200
VMWLLLLSIC LGSLTYVTAV LGVCLSSLGV PSYCDGVREL YINSSNVTTM 2250
DFCQGYFPCS VCLSGLDSLD SYPALETIQV TISSYKLDLT FLGLAAEWLL 2300
AYMLFTKFFY LLGLSAIMQA FFGYFASHFI SNSWLMWFII SIVQMAPVSA 2350
MVRMYIFFAS FYYVWKSYVH IMDGCTSSTC MMCYKRNRAT RVECTTIVNG 2400
VKRSFYVYAN GGRGFCKAHN WNCLNCDTFC AGSTFISDEV ARDLSLQFKR 2450
PINPTDQSAY VVDSVTVKNG ALHLYFDKAG QKTYERHPLS HFVNLDNLRA 2500
NNTKGSLPIN VIVFDGKSKC EESAAKSASV YYSQLMCQPI LLLDQALVSD 2550
VGDSTEVSVK MFDAYVDTFS ATFSVPMEKL KALVATAHSE LAKGVALDGV 2600
LSTFVSAARQ GVVDTDVDTK DVIECLKLSH HSDIEVTGDS CNNFMLTYNK 2650
VENMTPRDLG ACIDCNARHI NAQVAKSHNV SLVWNVKDYM SLSEQLRKQI 2700
RSAAKKNNIP FRLTCATTRQ VVNVITTKIS LKGGKVVSTW FKLLLKVTLL 2750
CVLAALFCYV IMPVHSLSVH DGYTNEIIGY KAIQDGVTRD IVSTDDCFAN 2800
KHAGFDSWFS QRGGSYRNDK NCPVVAAIIT REIGFIVPGL PGTVLRALNG 2850
DFLHFLPRVF SAVGNICYTP SKLIEYSDFA TSACVLAAEC TIFKDAMGKP 2900
VPYCYDTNLL EGSISYSELR PDTRYVLMDG SIIQFPNTYL EGSVRVVTTF 2950
DAEYCRHGTC ERSEVGVCLS TSGRWVLNNE HYRALPGVFC GVDAMNLIAN 3000
IFTPLVQPVG ALDVSASVVA GGIIAILVTC AAYYFMKFRR AFGEYNHVVA 3050
ANALLFLMSF TILCLAPAYS FLPGVYSIFY LYLTFYFTND VSFLAHLQWF 3100
AMFSPIVPFW ITAIYVFCIS LKHFHWFFSN YLKKRVMFNG VTFSTFEEAA 3150
LCTFLLNKEM YLRLRSETLL PLTQYNRYLA LYNKYKYFSG ALDTTSYREA 3200
ACCHLAKALN DFSNSGADVL YQPPQTSITS AVLQSGFRKM AFPSGKVEGC 3250
MVQVTCGTTT LNGLWLDDTV YCPRHVVCTA EDMLNPNYDD LLIRKSNHSF 3300
LVQAGNVQLR VIGHSMQNCL LRLKVDTSNP KTPKYKFVRI QPGQTFSVLA 3350
CYNGSPSGVY QCAMRPNHTI KGSFLNGSCG SVGFNIDYDC VSFCYMHHME 3400
LPTGVHAGTD LEGKFYGPFV DRQTAQAAGT DTTITLNVLA WLYAAVINGD 3450
RWFLNRFTTT LNDFNLVAMK YNYEPLTQDH VDILGPLSAQ TGIAVLDMCA 3500
ALKELLQNGM NGRTILGSTI LEDEFTPFDV VRQCSGVTFQ GKFKKIVKGT 3550
HHWMLLTFLT SLLILVQSTQ WSLFFFVYEN AFLPFALGIM AVAACAMLLV 3600
KHKHAFLCLF LLPSLATVAY FNMVYMPASW VMRIMTWLEL ADTSLSGYRL 3650
KDCVMYASAL VLLILMTART VYDDAARRVW TLMNVITLVY KVYYGNSLDQ 3700
AISMWALVIS VTSNYSGVVT TIMFLARAIV FVCVEYYPLL FITGNTLQCI 3750
MLVYCFLGYC CCCYFGLFCL LNRYFRLTLG VYDYLVSTQE FRYMNSQGLL 3800
PPKSSIDAFK LNIKLLGIGG KPCIKVATVQ SKMSDVKCTS VVLLSVLQQL 3850
RVESSSKLWA QCVQLHNDIL LAKDTTEAFE KMVSLLSVLL SMQGAVDINK 3900
LCEEMLDNRA TLQAIASEFS SLPSYAAYAT AQEAYEQAVS NGDSEVVLKK 3950
LKKSLNVAKS EFDHDAAMQR KLEKMADQAM TQMYKQARSE DKRAKVTSAM 4000
QTMLFTMLRK LDNDALNNII NNARDGCVPL NIIPLTTAAK LMVVVPDYGT 4050
YKNTCDGNTF TYASALWEIQ QVVDADSKIV QLSEINMDNS PNLAWPLIVT 4100
ALRANSAVKL QNNELSPVAL RQMSCAAGTT QTACTDDNAL AYYNNAKGGR 4150
FVLALLSDHQ DLKWARFPKS DGTGTIYTEL EPPCRFVTDT PKGPKVKYLY 4200
FIKGLNNLNR GMVLGSLAAT VRLQAGNATE VPANSTVLSF CAFAVDPAKA 4250
YKDYLASGGQ PITNCVKMLC THTGTGQAIT VTPEANMDQE SFGGASCCLY 4300
CRCHIDHPNP KGFCDLKGKY VQIPTTCAND PVGFTLRNTV CTVCGMWKGY 4350
GCSCDQLREP MMQSADASTF LNRVCGVSAA RLTPCGTGTS TDVVYRAFDI 4400
YNEKVAGFAK FLKTNCCRFQ EKDEEGNLLD SYFVVKRHTM SNYQHEETIY 4450
NLIKECPAVA VHDFFKFRVD GDMVPHISRQ RLTKYTMADL VYALRHFDEG 4500
NCDTLKEILV TYNCCDDNYF NKKDWYDFVE NPDVLRVYAN LGERVRRALL 4550
KTVQFCDAMR DAGIVGVLTL DNQDLNGNWY DFGDFVQVAP GCGVPIVDSY 4600
YSLLMPILTL TKALAAESHM DADLAKPLVK WDLLKYDFTE ERLCLFDRYF 4650
KYWDQTYHPN CINCLDDRCI LHCANFNVLF STVFPPTSFG PLVRKIFVDG 4700
VPFVVSTGYH FRELGVVHNQ DVNLHSSRLS FKELLVYAAD PAMHAASGNL 4750
LLDKRTTCFS VAALTNNVAF QTVKPGNFNK DFYDFAVSKG FFKEGSSVEL 4800
KHFFFAQDGN AAISDYDYYR YNLPTMCDIR QLLFVVEVVD KYFDCYDGGC 4850
INANQVIVNN LDKSAGFPFN KWGKARLYYD SMSYEDQDAL FAYTKRNVIP 4900
TITQMNLKYA ISAKNRARTV AGVSICSTMT NRQFHQKLLK SIAATRGATV 4950
VIGTSKFYGG WHNMLKTVYS DVESPHLMGW DYPKCDRAMP NMLRIMASLI 5000
LARKHSTCCN LSHRFYRLAN ECAQVLSEMV MCGGSLYVKP GGTSSGDATT 5050
AYANSVFNIC QAVTANVNAL LSTDGNKIAD KYVRNLQHRL YECLYRNRDV 5100
DHEFVDEFYA YLRKHFSMMI LSDDAVVCYN SNYAAQGLVA SIKNFKAVLY 5150
YQNNVFMSEA KCWTETDLTR GPHEFCSQHT MLVKQGDDYV YLPYPDPSRI 5200
LGAGCFVDDI VKTDGTLMIE RFVSLAIDAY PLTKHPNQEY ADVFHLYLQY 5250
IRKLHDELTG HMLDMYSVML TNDNTSRYWE PEFYEAMYTP HTVLQAVGAC 5300
VLCNSQTSLR CGACIRRPFL CCKCCYDHVI STSHKLVLSV NPYVCNAPGC 5350
DVTDVTQLYL GGMSYYCKSH KPPISFPLCA NGQVFGLYKN TCVGSDNVTD 5400
FNAIATCDWT NAGDYILANT CTERLKLFAA ETLKATEETF KLSYGIATVR 5450
EVLSDRELYL SWEVGKPRPP LNRNYVFTGY RVTKNSKVQI GEYTFEKGDY 5500
GDAVVYRGTT TYKLNVGDYF VLTSHTVMPL SAPTLVPQEH YVRITGLYPT 5550
LNISNEFSSN VANYQKIGMQ KYSTLQGPPG TGKSHFAIGL ALYYPSARIV 5600
YTACSHAAVD ALCEKALKYL PIDKCSRIIP ARARVECFDK FKVNSTLEQY 5650
VFCTVNALPE TTADIVVFDE ISMATNYDLS VVNARLRAKH YVYIGDPAQL 5700
PAPRTLLTKG TLEPEYFNSV CRLMKTIGPD MFLGTCRRCP AEIVDTVSAL 5750
VYDNKLKAHK EKSAQCFKMY YKGVITHDVS SAINRPQIGV VREFLTRNPA 5800
WRKAVFISPY NSQNAVASKI LGLPTQTVDS SQGSEYDYVI FTQTTETAHS 5850
CNVNRFNVAI TRAKIGILCI MSDRDLYDKL QFTSLEVPRR NVATLQAENV 5900
TGLFKDCSKI ITGLHPTQAP THLSVDTKFK TEGLCVDIPG IPKDMTYRRL 5950
ISMMGFKMNY QVNGYPNMFI TREEAIRHVR AWIGFDVEGC HATRDAVGTN 6000
LPLQLGFSTG VNLVAVPTGY VDTENSTEFT RVNAKPPPGD QFKHLIPLMY 6050
KGLPWNVVRI KIVQMLSDTL KGLSDRVVFV LWAHGFELTS MKYFVKIGPE 6100
RTCCLCDKRA TCFSTSSDTY ACWNHSVGFD YVYNPFMIDV QQWGFTGNLQ 6150
SNHDQHCQVH GNAHVASCDA IMTRCLAVHE CFVKRVDWSV EYPIIGDELK 6200
INAACRKVQH MVVKSALLAD KFTVLHDIGN PKAIRCVPQA EVDWKFYDAQ 6250
PCSDKAYKIE ELFYSYATHH DKFTDGVCLF WNCNVDRYPA NAIVCRFDTR 6300
VLSNLNLPGC DGGSLYVNKH AFHTPAFDKS AFTHLKQLPF FYYSDSPCES 6350
HGKQVVSDID YVPLKSATCI TRCNLGGAVC RHHANEYRQY LDAYNMMISA 6400
GFSLWIYKQF DTYNLWNTFT KLQSLENVAY NVVNKGHFDG QSGEAPVSII 6450
NNAVYTKVDG IDVEIFENKT TLPVNVAFEL WAKRNIKPVP EIKILNNLGV 6500
DIAANNVIWD YKREAPAHVS TIGVCTMTDI AKKPTESACS SLIVLFDGRV 6550
EGQVDFFRNA RNGVLITEGS VKGLTPSKGP AQASVNGVTL IGESVKTQFN 6600
YFKKVDGIIQ QLPETYFTQS RDLEDFKPRS QMETDFLELA MDEFIQRYKL 6650
EGYAFEHIVY GDFSHGQLGG LHLMIGLAKR SQDSLLKLED FIPMDSTVKN 6700
YFITDAQTGS SKCVCSVIDL LLDDFVEIIK SQDLSVVSKV VKVTIDYAEI 6750
SFMLWCKDGH VETFYPKLQA SQAWQPGVAM PNLYKMQRML LEKCDLQNYG 6800
ENAVIPKGIM MNVAKYTQLC QYLNTLTLAV PYNMRVIHFG AGSDKGVAPG 6850
TAVLRQWLPT GTLLVDSDLN DFVSDADSTL IGDCATVHTA NKWDLIISDM 6900
YDPKTKHVLK DNDSKEGFFT YLCGFIKQKL ALGGSVAVKI TEHSWNADLY 6950
KLMGHFSWWT AFVTNVNASS SEAFLIGVNY LGKPKEQIDG YTMHANYIFW 7000
RNTNPIQLSS YSLFDMSKFP LKLRGTAVMS LKENQINDMI YSLLEKGRLI 7050
IRENNRVVVS SDILVNN 7067

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,067
Mass (Da):789,496
Last modified:June 10, 2008 - v1
Checksum:i9EE66BBA1B5E6339
GO
Isoform Replicase polyprotein 1a (identifier: P0C6F8-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6F8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,376
Mass (Da):485,585
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ022305 Genomic RNA. Translation: AAY88865.2.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ022305 Genomic RNA. Translation: AAY88865.2 .

3D structure databases

ProteinModelPortali P0C6W2.
SMRi P0C6W2. Positions 13-127, 819-930, 997-1171, 1325-1463, 1535-1846, 3235-3540, 3830-3913, 3915-4105, 4112-4224, 4234-4356, 6424-6767.
ModBasei Search...

Protein family/group databases

MEROPSi C16.009.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate HKU3-1.

Entry informationi

Entry nameiR1AB_BCHK3
AccessioniPrimary (citable) accession number: P0C6W2
Secondary accession number(s): Q3LZX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus HKU3 is highly similar to SARS-CoV (SARS-like).

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi