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P0C6W2

- R1AB_BCHK3

UniProt

P0C6W2 - R1AB_BCHK3

Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity
    The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.By similarity
    The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
    Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.By similarity

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    ATP + H2O = ADP + phosphate.
    TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei179 – 1802Cleavage; by PL-PROBy similarity
    Sitei818 – 8192Cleavage; by PL-PROBy similarity
    Active sitei1645 – 16451For PL-PRO activityPROSITE-ProRule annotation
    Active sitei1806 – 18061For PL-PRO activityPROSITE-ProRule annotation
    Sitei3234 – 32352Cleavage; by 3CL-PROBy similarity
    Active sitei3275 – 32751For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3379 – 33791For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3540 – 35412Cleavage; by 3CL-PROBy similarity
    Sitei3830 – 38312Cleavage; by 3CL-PROBy similarity
    Sitei3913 – 39142Cleavage; by 3CL-PROBy similarity
    Sitei4111 – 41122Cleavage; by 3CL-PROBy similarity
    Sitei4224 – 42252Cleavage; by 3CL-PROBy similarity
    Sitei4363 – 43642Cleavage; by 3CL-PROBy similarity
    Sitei5295 – 52962Cleavage; by 3CL-PROBy similarity
    Sitei5896 – 58972Cleavage; by 3CL-PROBy similarity
    Sitei6423 – 64242Cleavage; by 3CL-PROBy similarity
    Sitei6769 – 67702Cleavage; by 3CL-PROBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1723 – 176038C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4298 – 431417By similarityAdd
    BLAST
    Zinc fingeri4341 – 435414By similarityAdd
    BLAST
    Nucleotide bindingi5577 – 55848ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. helicase activity Source: UniProtKB-KW
    6. methyltransferase activity Source: InterPro
    7. omega peptidase activity Source: InterPro
    8. RNA binding Source: UniProtKB-KW
    9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    10. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    2. induction by virus of host autophagy Source: UniProtKB-KW
    3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    4. suppression by virus of host gene expression Source: UniProtKB-KW
    5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    7. transcription, DNA-templated Source: InterPro
    8. viral protein processing Source: InterPro
    9. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Protein family/group databases

    MEROPSiC16.009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Short name:
    pp1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 15 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    Leader protein
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p65 homolog
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name:
    PL-PRO
    Non-structural protein 4
    Short name:
    nsp4
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    nsp5
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Non-structural protein 8
    Short name:
    nsp8
    Non-structural protein 9
    Short name:
    nsp9
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    nsp12
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    nsp13
    Exoribonuclease (EC:3.1.13.-)
    Short name:
    ExoN
    Alternative name(s):
    nsp14
    Alternative name(s):
    NendoU
    nsp15
    Alternative name(s):
    nsp16
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiBat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3)
    Taxonomic identifieri442736 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiRhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399]
    ProteomesiUP000007450: Genome

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
    Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. host cell membrane Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Non-structural protein 1By similarityPRO_0000291335Add
    BLAST
    Chaini180 – 818639Non-structural protein 2By similarityPRO_0000291336Add
    BLAST
    Chaini819 – 27341916Non-structural protein 3By similarityPRO_0000291337Add
    BLAST
    Chaini2735 – 3234500Non-structural protein 4By similarityPRO_0000291338Add
    BLAST
    Chaini3235 – 35403063C-like proteinaseBy similarityPRO_0000291339Add
    BLAST
    Chaini3541 – 3830290Non-structural protein 6By similarityPRO_0000291340Add
    BLAST
    Chaini3831 – 391383Non-structural protein 7By similarityPRO_0000291341Add
    BLAST
    Chaini3914 – 4111198Non-structural protein 8By similarityPRO_0000291342Add
    BLAST
    Chaini4112 – 4224113Non-structural protein 9By similarityPRO_0000291343Add
    BLAST
    Chaini4225 – 4363139Non-structural protein 10By similarityPRO_0000291344Add
    BLAST
    Chaini4364 – 5295932RNA-directed RNA polymeraseBy similarityPRO_0000291345Add
    BLAST
    Chaini5296 – 5896601HelicaseBy similarityPRO_0000291346Add
    BLAST
    Chaini5897 – 6423527ExoribonucleaseBy similarityPRO_0000291347Add
    BLAST
    Chaini6424 – 6769346Uridylate-specific endoribonucleaseBy similarityPRO_0000291348Add
    BLAST
    Chaini6770 – 7067298Putative 2'-O-methyl transferaseBy similarityPRO_0000291349Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C6W2.
    SMRiP0C6W2. Positions 13-127, 819-930, 997-1171, 1325-1463, 1535-1846, 3235-3540, 3830-3913, 3915-4105, 4112-4224, 4234-4356, 6424-6767.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2197 – 221721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2298 – 231821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2345 – 236521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2744 – 276421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2986 – 300621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3016 – 303621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3048 – 306821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3071 – 309121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3099 – 311921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3136 – 315621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3558 – 357821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3580 – 360021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3606 – 362621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3652 – 367221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3679 – 369820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3722 – 374221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3750 – 377021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini998 – 1164167MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1605 – 1869265Peptidase C16PROSITE-ProRule annotationAdd
    BLAST
    Domaini3235 – 3540306Peptidase C30PROSITE-ProRule annotationAdd
    BLAST
    Domaini4975 – 5137163RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini5296 – 537984CV MBDAdd
    BLAST
    Domaini5552 – 5733182(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini5734 – 5903170(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2086 – 2365280HD1By similarityAdd
    BLAST
    Regioni2749 – 3156408HD2By similarityAdd
    BLAST
    Regioni3558 – 3770213HD3By similarityAdd
    BLAST
    Regioni4906 – 5208303RNA-directed RNA polymeraseBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi930 – 99667Glu-richAdd
    BLAST
    Compositional biasi2204 – 22074Poly-Leu
    Compositional biasi3760 – 37634Poly-Cys

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1723 – 176038C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4298 – 431417By similarityAdd
    BLAST
    Zinc fingeri4341 – 435414By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1ab (identifier: P0C6W2-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MESLVLGVNE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKNGT     50
    CGLVELEKGV LPQLEQPYVF IKRSDALSTN HGHKVVELVA ELDGIQFGRS 100
    GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG 150
    TDPIEDYEQN WNTKHGSGAL RELTRELNGG VVTRYVDNNF CGPDGYPLEC 200
    IKDFLARAGK SMCTLSEQLD YIESKRGVYC CREHEHEIVW FTERSEKSYE 250
    HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI 300
    RSVYPVATPQ ECNDMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV 350
    CEGPTTCGYL PTNAVVKMPC PACQDPEVGP EHSVADYHNH SNIETRLRKG 400
    GRTKCFGGCV FSYVGCYNKR AYWVPRASAN IGANHTGITG ENVETLNEDL 450
    LEILNRERVN INIVGDFRFN EEVAIILASF SASPSAFIET VKGLDYKSFK 500
    VIVESCGNYK VTNGKPVTGA WNIGQQRSIL TPLCGFPSQA AGVIRSIFSR 550
    TLDAANHSIL DLQRAAVTTL DGISEQSLRL VDAMVYTSDL LTNSVVVMAY 600
    VTGGLVQQTM QWLSNMLGTA VDKLKPVFTW VEAKLSAGVE FLRDAWEILK 650
    FLITGVFDVI KGQIQVATDN IKECVKIFLG VVNKALEMCL DQVTIAGTKL 700
    RALNLGEVFI AQSRGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDAHDTV 750
    LTSEEVVLKS GELEALETPI DSFTSGAVVG TPVCINGLML LELENKEQYC 800
    ALSPGLLATN NVFRLKGGAP VKGVTFGEDT VLEVQGYKNV KITFELDVRV 850
    DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTPMGIDLDE 900
    WSVATFYLFD DAGEEKLSSR MYCSFYPPDE EEDCEECEDE EETCEHEYGT 950
    EDDYKGLPLE FGASTETPHV EEEEEEEDWL DDAIEAEPEP EPLPEEPVNQ 1000
    FVGYLKLTDN VAIKCIDIVK EAQSAKPTVI VNAANTHLKH GGGVAGALNK 1050
    ATNGAMQNES DEYIRQNGPL TVGGSCLLSG HNLAEKCLHV VGPNLNAGED 1100
    VQLLKRAYEN FNSQDVLLAP LLSAGIFGAK PLQSLKMCVE IVRTQVYLAV 1150
    NDKSLYDQIV LDYLDSLKPK VESPNKEEEP KLEEPKAVQP VAEKPVDVKP 1200
    KIKACIDEVT TTLEETKFLT NKLLLFADIN GKLYQDSQNM LRGEDMSFLE 1250
    KDAPYIVGDV ITSGDITCVI IPAKKSGGTT EMLARALKEV PVAEYITTYP 1300
    GQGCAGYTLE EAKTALKKCK SAFYVLPSET PNEKEEVLGT VSWNLREMLA 1350
    HAEETRKLMP ICLDVRAIMA TIQRKYKGIK VQEGIVDYGV RFFFYTSKEP 1400
    VASIITKLNS LNEPLVTMPI GYVTHGLNLE EAARCMRSLK APAVVSVSSP 1450
    DAVTAYNGYL TSSSKTPEEY FVETTSLAGS YRDWSYSGQR TELGVEFLKR 1500
    GDKIVYHTTG SPIEFHLDGE VLPLDKLKSL LSLREVKTIK VFTTVDNTNL 1550
    HTHIVDMSMT YGQQFGPTYL DGADVTKIKP HVNHEGKTFF VLPSDDTLRS 1600
    EAFEYYHTID ESFLGRYMSA LNHTKKWKFP QVGGLTSIKW ADNNCYLSSV 1650
    LLALQQVEVK FNAPALQEAY YRARAGDAAN FCALILAYSN KTVGELGDVR 1700
    ETMTHLLQHA NLESAKRVLN VVCKHCGQKT TTLKGVEAVM YMGTLSYDEL 1750
    KTGVSIPCVC GRNATQYLVQ QESSFVMMSA PPAEYKLQQG AFLCANEYTG 1800
    NYQCGHYTHI TAKETLYRVD GAHLTKMSEY KGPVTDVFYK ETSYTTAIKP 1850
    VSYKLDGVTY TEIEPKLDGY YKKGNAYYTE QPIDLVPTQP MPNASFDNFK 1900
    LTCSNTKFAD DLNQMTGFKK PASRELTVTF FPDLNGDVVA IDYRHYSTSF 1950
    KKGAKLVHKP ILWHINQTTN KTTYKPNIWC LRCLWSTKPV DTSNSFEVLV 2000
    VEDTQGMDNL ACESQTTTSE EVVENPTVQK EIIECDVKTT EVVGNVILKP 2050
    SEEGVKVTQE LGHEDLMAAY VEETSITIKK PNELSLALGL KTLATHGAAA 2100
    INSVPWSKIL AYVKPFLGQT AVITSNCIKK CVQRVFSNYM PYVITLLFQL 2150
    CTFTKSTNSR IKASLPTTIA KNSVKSVAKL CLDVCINYVK SPKFSKLFTI 2200
    VMWLLLLSIC LGSLTYVTAV LGVCLSSLGV PSYCDGVREL YINSSNVTTM 2250
    DFCQGYFPCS VCLSGLDSLD SYPALETIQV TISSYKLDLT FLGLAAEWLL 2300
    AYMLFTKFFY LLGLSAIMQA FFGYFASHFI SNSWLMWFII SIVQMAPVSA 2350
    MVRMYIFFAS FYYVWKSYVH IMDGCTSSTC MMCYKRNRAT RVECTTIVNG 2400
    VKRSFYVYAN GGRGFCKAHN WNCLNCDTFC AGSTFISDEV ARDLSLQFKR 2450
    PINPTDQSAY VVDSVTVKNG ALHLYFDKAG QKTYERHPLS HFVNLDNLRA 2500
    NNTKGSLPIN VIVFDGKSKC EESAAKSASV YYSQLMCQPI LLLDQALVSD 2550
    VGDSTEVSVK MFDAYVDTFS ATFSVPMEKL KALVATAHSE LAKGVALDGV 2600
    LSTFVSAARQ GVVDTDVDTK DVIECLKLSH HSDIEVTGDS CNNFMLTYNK 2650
    VENMTPRDLG ACIDCNARHI NAQVAKSHNV SLVWNVKDYM SLSEQLRKQI 2700
    RSAAKKNNIP FRLTCATTRQ VVNVITTKIS LKGGKVVSTW FKLLLKVTLL 2750
    CVLAALFCYV IMPVHSLSVH DGYTNEIIGY KAIQDGVTRD IVSTDDCFAN 2800
    KHAGFDSWFS QRGGSYRNDK NCPVVAAIIT REIGFIVPGL PGTVLRALNG 2850
    DFLHFLPRVF SAVGNICYTP SKLIEYSDFA TSACVLAAEC TIFKDAMGKP 2900
    VPYCYDTNLL EGSISYSELR PDTRYVLMDG SIIQFPNTYL EGSVRVVTTF 2950
    DAEYCRHGTC ERSEVGVCLS TSGRWVLNNE HYRALPGVFC GVDAMNLIAN 3000
    IFTPLVQPVG ALDVSASVVA GGIIAILVTC AAYYFMKFRR AFGEYNHVVA 3050
    ANALLFLMSF TILCLAPAYS FLPGVYSIFY LYLTFYFTND VSFLAHLQWF 3100
    AMFSPIVPFW ITAIYVFCIS LKHFHWFFSN YLKKRVMFNG VTFSTFEEAA 3150
    LCTFLLNKEM YLRLRSETLL PLTQYNRYLA LYNKYKYFSG ALDTTSYREA 3200
    ACCHLAKALN DFSNSGADVL YQPPQTSITS AVLQSGFRKM AFPSGKVEGC 3250
    MVQVTCGTTT LNGLWLDDTV YCPRHVVCTA EDMLNPNYDD LLIRKSNHSF 3300
    LVQAGNVQLR VIGHSMQNCL LRLKVDTSNP KTPKYKFVRI QPGQTFSVLA 3350
    CYNGSPSGVY QCAMRPNHTI KGSFLNGSCG SVGFNIDYDC VSFCYMHHME 3400
    LPTGVHAGTD LEGKFYGPFV DRQTAQAAGT DTTITLNVLA WLYAAVINGD 3450
    RWFLNRFTTT LNDFNLVAMK YNYEPLTQDH VDILGPLSAQ TGIAVLDMCA 3500
    ALKELLQNGM NGRTILGSTI LEDEFTPFDV VRQCSGVTFQ GKFKKIVKGT 3550
    HHWMLLTFLT SLLILVQSTQ WSLFFFVYEN AFLPFALGIM AVAACAMLLV 3600
    KHKHAFLCLF LLPSLATVAY FNMVYMPASW VMRIMTWLEL ADTSLSGYRL 3650
    KDCVMYASAL VLLILMTART VYDDAARRVW TLMNVITLVY KVYYGNSLDQ 3700
    AISMWALVIS VTSNYSGVVT TIMFLARAIV FVCVEYYPLL FITGNTLQCI 3750
    MLVYCFLGYC CCCYFGLFCL LNRYFRLTLG VYDYLVSTQE FRYMNSQGLL 3800
    PPKSSIDAFK LNIKLLGIGG KPCIKVATVQ SKMSDVKCTS VVLLSVLQQL 3850
    RVESSSKLWA QCVQLHNDIL LAKDTTEAFE KMVSLLSVLL SMQGAVDINK 3900
    LCEEMLDNRA TLQAIASEFS SLPSYAAYAT AQEAYEQAVS NGDSEVVLKK 3950
    LKKSLNVAKS EFDHDAAMQR KLEKMADQAM TQMYKQARSE DKRAKVTSAM 4000
    QTMLFTMLRK LDNDALNNII NNARDGCVPL NIIPLTTAAK LMVVVPDYGT 4050
    YKNTCDGNTF TYASALWEIQ QVVDADSKIV QLSEINMDNS PNLAWPLIVT 4100
    ALRANSAVKL QNNELSPVAL RQMSCAAGTT QTACTDDNAL AYYNNAKGGR 4150
    FVLALLSDHQ DLKWARFPKS DGTGTIYTEL EPPCRFVTDT PKGPKVKYLY 4200
    FIKGLNNLNR GMVLGSLAAT VRLQAGNATE VPANSTVLSF CAFAVDPAKA 4250
    YKDYLASGGQ PITNCVKMLC THTGTGQAIT VTPEANMDQE SFGGASCCLY 4300
    CRCHIDHPNP KGFCDLKGKY VQIPTTCAND PVGFTLRNTV CTVCGMWKGY 4350
    GCSCDQLREP MMQSADASTF LNRVCGVSAA RLTPCGTGTS TDVVYRAFDI 4400
    YNEKVAGFAK FLKTNCCRFQ EKDEEGNLLD SYFVVKRHTM SNYQHEETIY 4450
    NLIKECPAVA VHDFFKFRVD GDMVPHISRQ RLTKYTMADL VYALRHFDEG 4500
    NCDTLKEILV TYNCCDDNYF NKKDWYDFVE NPDVLRVYAN LGERVRRALL 4550
    KTVQFCDAMR DAGIVGVLTL DNQDLNGNWY DFGDFVQVAP GCGVPIVDSY 4600
    YSLLMPILTL TKALAAESHM DADLAKPLVK WDLLKYDFTE ERLCLFDRYF 4650
    KYWDQTYHPN CINCLDDRCI LHCANFNVLF STVFPPTSFG PLVRKIFVDG 4700
    VPFVVSTGYH FRELGVVHNQ DVNLHSSRLS FKELLVYAAD PAMHAASGNL 4750
    LLDKRTTCFS VAALTNNVAF QTVKPGNFNK DFYDFAVSKG FFKEGSSVEL 4800
    KHFFFAQDGN AAISDYDYYR YNLPTMCDIR QLLFVVEVVD KYFDCYDGGC 4850
    INANQVIVNN LDKSAGFPFN KWGKARLYYD SMSYEDQDAL FAYTKRNVIP 4900
    TITQMNLKYA ISAKNRARTV AGVSICSTMT NRQFHQKLLK SIAATRGATV 4950
    VIGTSKFYGG WHNMLKTVYS DVESPHLMGW DYPKCDRAMP NMLRIMASLI 5000
    LARKHSTCCN LSHRFYRLAN ECAQVLSEMV MCGGSLYVKP GGTSSGDATT 5050
    AYANSVFNIC QAVTANVNAL LSTDGNKIAD KYVRNLQHRL YECLYRNRDV 5100
    DHEFVDEFYA YLRKHFSMMI LSDDAVVCYN SNYAAQGLVA SIKNFKAVLY 5150
    YQNNVFMSEA KCWTETDLTR GPHEFCSQHT MLVKQGDDYV YLPYPDPSRI 5200
    LGAGCFVDDI VKTDGTLMIE RFVSLAIDAY PLTKHPNQEY ADVFHLYLQY 5250
    IRKLHDELTG HMLDMYSVML TNDNTSRYWE PEFYEAMYTP HTVLQAVGAC 5300
    VLCNSQTSLR CGACIRRPFL CCKCCYDHVI STSHKLVLSV NPYVCNAPGC 5350
    DVTDVTQLYL GGMSYYCKSH KPPISFPLCA NGQVFGLYKN TCVGSDNVTD 5400
    FNAIATCDWT NAGDYILANT CTERLKLFAA ETLKATEETF KLSYGIATVR 5450
    EVLSDRELYL SWEVGKPRPP LNRNYVFTGY RVTKNSKVQI GEYTFEKGDY 5500
    GDAVVYRGTT TYKLNVGDYF VLTSHTVMPL SAPTLVPQEH YVRITGLYPT 5550
    LNISNEFSSN VANYQKIGMQ KYSTLQGPPG TGKSHFAIGL ALYYPSARIV 5600
    YTACSHAAVD ALCEKALKYL PIDKCSRIIP ARARVECFDK FKVNSTLEQY 5650
    VFCTVNALPE TTADIVVFDE ISMATNYDLS VVNARLRAKH YVYIGDPAQL 5700
    PAPRTLLTKG TLEPEYFNSV CRLMKTIGPD MFLGTCRRCP AEIVDTVSAL 5750
    VYDNKLKAHK EKSAQCFKMY YKGVITHDVS SAINRPQIGV VREFLTRNPA 5800
    WRKAVFISPY NSQNAVASKI LGLPTQTVDS SQGSEYDYVI FTQTTETAHS 5850
    CNVNRFNVAI TRAKIGILCI MSDRDLYDKL QFTSLEVPRR NVATLQAENV 5900
    TGLFKDCSKI ITGLHPTQAP THLSVDTKFK TEGLCVDIPG IPKDMTYRRL 5950
    ISMMGFKMNY QVNGYPNMFI TREEAIRHVR AWIGFDVEGC HATRDAVGTN 6000
    LPLQLGFSTG VNLVAVPTGY VDTENSTEFT RVNAKPPPGD QFKHLIPLMY 6050
    KGLPWNVVRI KIVQMLSDTL KGLSDRVVFV LWAHGFELTS MKYFVKIGPE 6100
    RTCCLCDKRA TCFSTSSDTY ACWNHSVGFD YVYNPFMIDV QQWGFTGNLQ 6150
    SNHDQHCQVH GNAHVASCDA IMTRCLAVHE CFVKRVDWSV EYPIIGDELK 6200
    INAACRKVQH MVVKSALLAD KFTVLHDIGN PKAIRCVPQA EVDWKFYDAQ 6250
    PCSDKAYKIE ELFYSYATHH DKFTDGVCLF WNCNVDRYPA NAIVCRFDTR 6300
    VLSNLNLPGC DGGSLYVNKH AFHTPAFDKS AFTHLKQLPF FYYSDSPCES 6350
    HGKQVVSDID YVPLKSATCI TRCNLGGAVC RHHANEYRQY LDAYNMMISA 6400
    GFSLWIYKQF DTYNLWNTFT KLQSLENVAY NVVNKGHFDG QSGEAPVSII 6450
    NNAVYTKVDG IDVEIFENKT TLPVNVAFEL WAKRNIKPVP EIKILNNLGV 6500
    DIAANNVIWD YKREAPAHVS TIGVCTMTDI AKKPTESACS SLIVLFDGRV 6550
    EGQVDFFRNA RNGVLITEGS VKGLTPSKGP AQASVNGVTL IGESVKTQFN 6600
    YFKKVDGIIQ QLPETYFTQS RDLEDFKPRS QMETDFLELA MDEFIQRYKL 6650
    EGYAFEHIVY GDFSHGQLGG LHLMIGLAKR SQDSLLKLED FIPMDSTVKN 6700
    YFITDAQTGS SKCVCSVIDL LLDDFVEIIK SQDLSVVSKV VKVTIDYAEI 6750
    SFMLWCKDGH VETFYPKLQA SQAWQPGVAM PNLYKMQRML LEKCDLQNYG 6800
    ENAVIPKGIM MNVAKYTQLC QYLNTLTLAV PYNMRVIHFG AGSDKGVAPG 6850
    TAVLRQWLPT GTLLVDSDLN DFVSDADSTL IGDCATVHTA NKWDLIISDM 6900
    YDPKTKHVLK DNDSKEGFFT YLCGFIKQKL ALGGSVAVKI TEHSWNADLY 6950
    KLMGHFSWWT AFVTNVNASS SEAFLIGVNY LGKPKEQIDG YTMHANYIFW 7000
    RNTNPIQLSS YSLFDMSKFP LKLRGTAVMS LKENQINDMI YSLLEKGRLI 7050
    IRENNRVVVS SDILVNN 7067

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:7,067
    Mass (Da):789,496
    Last modified:June 10, 2008 - v1
    Checksum:i9EE66BBA1B5E6339
    GO
    Isoform Replicase polyprotein 1a (identifier: P0C6F8-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform can be found in the external entry P0C6F8.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by conventional translation.

    Length:4,376
    Mass (Da):485,585
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ022305 Genomic RNA. Translation: AAY88865.2.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ022305 Genomic RNA. Translation: AAY88865.2 .

    3D structure databases

    ProteinModelPortali P0C6W2.
    SMRi P0C6W2. Positions 13-127, 819-930, 997-1171, 1325-1463, 1535-1846, 3235-3540, 3830-3913, 3915-4105, 4112-4224, 4234-4356, 6424-6767.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C16.009.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR009466. NSP11.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF06478. Corona_RPol_N. 1 hit.
    PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEi PS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate HKU3-1.

    Entry informationi

    Entry nameiR1AB_BCHK3
    AccessioniPrimary (citable) accession number: P0C6W2
    Secondary accession number(s): Q3LZX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Bat coronavirus HKU3 is highly similar to SARS-CoV (SARS-like).

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3