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P0C6W1

- R1AB_BC133

UniProt

P0C6W1 - R1AB_BC133

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Protein
Replicase polyprotein 1ab
Gene
rep, 1a-1b
Organism
Bat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.1 Publication
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.1 Publication
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.1 Publication
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.1 Publication
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.1 Publication
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.1 Publication
Nsp9 is a ssRNA-binding protein By similarity.1 Publication
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.1 Publication
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei195 – 1962Cleavage Reviewed prediction
Sitei847 – 8482Cleavage; by PL-PRO Reviewed prediction
Active sitei1641 – 16411For PL-PRO activity By similarity
Active sitei1807 – 18071For PL-PRO activity By similarity
Sitei2791 – 27922Cleavage; by PL-PRO Reviewed prediction
Sitei3298 – 32992Cleavage; by 3CL-PRO Reviewed prediction
Active sitei3339 – 33391For 3CL-PRO activity By similarity
Active sitei3446 – 34461For 3CL-PRO activity By similarity
Sitei3604 – 36052Cleavage; by 3CL-PRO Reviewed prediction
Sitei3896 – 38972Cleavage; by 3CL-PRO Reviewed prediction
Sitei3979 – 39802Cleavage; by 3CL-PRO Reviewed prediction
Sitei4178 – 41792Cleavage; by 3CL-PRO Reviewed prediction
Sitei4288 – 42892Cleavage; by 3CL-PRO Reviewed prediction
Sitei4427 – 44282Cleavage; by 3CL-PRO Reviewed prediction
Sitei5361 – 53622Cleavage; by 3CL-PRO Reviewed prediction
Sitei5959 – 59602Cleavage; by 3CL-PRO Reviewed prediction
Sitei6482 – 64832Cleavage; by 3CL-PRO Reviewed prediction
Sitei6824 – 68252Cleavage; by 3CL-PRO Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1721 – 175838C4-type By similarity
Add
BLAST
Zinc fingeri4362 – 437817 By similarity
Add
BLAST
Zinc fingeri4404 – 441714 By similarity
Add
BLAST
Nucleotide bindingi5643 – 56508ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. endonuclease activity Source: UniProtKB-KW
  6. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  7. helicase activity Source: UniProtKB-KW
  8. methyltransferase activity Source: InterPro
  9. omega peptidase activity Source: InterPro
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  5. suppression by virus of host translation Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral RNA genome replication Source: InterPro
  9. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)
Taxonomic identifieri389230 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirusunclassified Betacoronavirus
Virus hostiTylonycteris pachypus (Lesser bamboo bat) [TaxID: 258959]
ProteomesiUP000007449: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2119 – 213921Helical; Reviewed prediction
Add
BLAST
Transmembranei2152 – 217221Helical; Reviewed prediction
Add
BLAST
Transmembranei2229 – 224921Helical; Reviewed prediction
Add
BLAST
Transmembranei2333 – 235321Helical; Reviewed prediction
Add
BLAST
Transmembranei2357 – 237721Helical; Reviewed prediction
Add
BLAST
Transmembranei2382 – 240221Helical; Reviewed prediction
Add
BLAST
Transmembranei2807 – 282721Helical; Reviewed prediction
Add
BLAST
Transmembranei3079 – 309921Helical; Reviewed prediction
Add
BLAST
Transmembranei3112 – 313221Helical; Reviewed prediction
Add
BLAST
Transmembranei3156 – 317621Helical; Reviewed prediction
Add
BLAST
Transmembranei3610 – 363021Helical; Reviewed prediction
Add
BLAST
Transmembranei3644 – 366421Helical; Reviewed prediction
Add
BLAST
Transmembranei3669 – 368921Helical; Reviewed prediction
Add
BLAST
Transmembranei3714 – 373421Helical; Reviewed prediction
Add
BLAST
Transmembranei3742 – 376221Helical; Reviewed prediction
Add
BLAST
Transmembranei3791 – 381121Helical; Reviewed prediction
Add
BLAST
Transmembranei3815 – 383521Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Non-structural protein 1 Reviewed prediction
PRO_0000290273Add
BLAST
Chaini196 – 847652Non-structural protein 2 Reviewed prediction
PRO_0000290274Add
BLAST
Chaini848 – 27911944Non-structural protein 3 Reviewed prediction
PRO_0000290275Add
BLAST
Chaini2792 – 3298507Non-structural protein 4 Reviewed prediction
PRO_0000290276Add
BLAST
Chaini3299 – 36043063C-like proteinase Reviewed prediction
PRO_0000290277Add
BLAST
Chaini3605 – 3896292Non-structural protein 6 Reviewed prediction
PRO_0000290278Add
BLAST
Chaini3897 – 397983Non-structural protein 7 Reviewed prediction
PRO_0000290279Add
BLAST
Chaini3980 – 4178199Non-structural protein 8 Reviewed prediction
PRO_0000290280Add
BLAST
Chaini4179 – 4288110Non-structural protein 9 Reviewed prediction
PRO_0000290281Add
BLAST
Chaini4289 – 4427139Non-structural protein 10 Reviewed prediction
PRO_0000290282Add
BLAST
Chaini4428 – 5361934RNA-directed RNA polymerase Reviewed prediction
PRO_0000290283Add
BLAST
Chaini5362 – 5959598Helicase Reviewed prediction
PRO_0000290284Add
BLAST
Chaini5960 – 6482523Exoribonuclease Reviewed prediction
PRO_0000290285Add
BLAST
Chaini6483 – 6824342Uridylate-specific endoribonuclease Reviewed prediction
PRO_0000290286Add
BLAST
Chaini6825 – 7126302Putative 2'-O-methyl transferase Reviewed prediction
PRO_0000290287Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Proteomic databases

PRIDEiP0C6W1.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6W1.
SMRiP0C6W1. Positions 3896-3979, 4228-4288, 4298-4418.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1159 – 1328170Macro
Add
BLAST
Domaini1600 – 1871272Peptidase C16
Add
BLAST
Domaini3299 – 3604306Peptidase C30
Add
BLAST
Domaini5041 – 5203163RdRp catalytic
Add
BLAST
Domaini5362 – 544584CV MBD
Add
BLAST
Domaini5618 – 5799182(+)RNA virus helicase ATP-binding
Add
BLAST
Domaini5800 – 5974175(+)RNA virus helicase C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2119 – 2402284HD1 By similarity
Add
BLAST
Regioni2807 – 3176370HD2 By similarity
Add
BLAST
Regioni3610 – 3835226HD3 By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi960 – 1059100Glu-rich
Add
BLAST
Compositional biasi5366 – 539126Cys-rich
Add
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W1-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MLSKAGVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTED MASKLTPWFE     50
DGETAFNQVS SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS 100
KHFLVNQLAY KDHVGAAMMR TTLNVKPLGM FFPYDSSLET GEHTFLLRKN 150
GLGGQLFRER PWDRKETPYV EILDDLEADP TGKYSQNLLK KLIGGDCIPV 200
DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS DRFIVLNKKL 250
YRVVWNVTRR NVPYSKQTAF TVVSVIQCDD KESVPEHTFT IGSQILMVSP 300
LKATNNKNFN LKQRLLHTFY GKEAVQQPGY IYHSAYVDCN ACGRGTWCTG 350
NAIQGFACDC GANYSANDVD LQSSGLVPKN ALFLANCPCA NNGACSHNAA 400
QVYSILDGKA CVEVGGKSFT LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR 450
IGDAIFTGCT GTWDKVVETA NLFLEKAQHS LNFCQQFALT EVVLAILSGT 500
TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI NIGANGVCNA 550
TINAPFIAFT GLGESFKKVA AIPWKICSNL KSALDYYCSN IMFRVFPYDI 600
PCDVNDFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SNACQTALSS 650
FLNACVAASR ATAGFISDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ 700
QVLDIMSKAM KLLHTNVSWA GTKLSAIIYE GREALLFNSG TYFCLSTKAK 750
TLQDQMNLVL PGDYNKKTLG ILDPVPNADT IDVTANSTVV DVVHGQLEPT 800
NEHGPSMIVG NYVLVSDKLF VRTDDEEFYP LCINGKVVST LFRLKGGMPS 850
KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE 900
DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN 950
MTFSINPVED VEEVEEFIED DYLSDELPIA DDEEAWTRAV EEVMPLDDIL 1000
VAEIELEEDL PLETALESVE AEVGESISDE LCVVETAKAQ EPSVESTDST 1050
PSTSTVVSEN DLSVKPMSRV AETGDVLEVE TAVVGGPVSD VTASVVTNDI 1100
VSVEQAQQCG VSSLPIQDEA SENQVHQVPD LQCTSETKVE IVQPRQDLRP 1150
RRLRKSKVDL SKYKHTVINN SVTLVLGDAI QIASLLPKCV LVNAANRHLK 1200
HGGGIAGAIN KASGGDVQEE SDEYISNSGP LHVGDSVLLK GYGLADAILR 1250
VVGPDARNNE DAALLKRCYK TFNKHTIVVT PLISSGIFSV DPKVSFEYLL 1300
ANVTTTTYVV VNNEDIYNTL ATPSKPDGLV YSFEGWRGTV RTAKNYGFTC 1350
FICTEYSANV KFLRTKGVDT TKKIQTVDGV SYYLYSARDA LTDVIAAANG 1400
CPGICAMPFG YVTHGLDLAQ SGNYVRQVKV PYVCLLASKE QIPIMNSDVA 1450
IQTPETAFIN NVTSNGGYHS WHLVSGDLIV KDVCYKKLLH WSGQTICYAD 1500
NKFYVVKNDV ALPFSDLEAC RAYLTSRAAQ QVNIEVLVTI DGVNFRTVIL 1550
NDATTFRKQL GATFYKGVDI SDALPTVKMG GESLFVADNL SESEEVVLKE 1600
YYGTSDVTFL QRYYSLQPLV QQWKFVVHDG VKSLKLSNYN CYINATIMMI 1650
DMLHDIKFVV PALQNAYLRY KGGDPYDFLA LIMAYGDCTF DNPDDEAKLL 1700
HTLLAKAELT VSAKMVWREW CTVCGIRDIE YTGMRACVYA GVNSMEELQS 1750
VFNETCVCGS VKHRQLVEHS TPWLLVSGLN EVKVSTSTDP VYRAFNVFQG 1800
VETSVGHYVH VRVKDGLFYK YDSGSLTKTS DMKCKMTSVW YPKVRYTADC 1850
NVVVYDLDGV TKVEVNPDLS NYYMKDGKYY TSKPTIKYSP ATILPGSVYS 1900
NSCLVGVDGT PGSDTISKFF NDLLGFDETK PISKKLTYSL LPNEDGDVLL 1950
SEFNNYNPVY KKGVMLKGKP ILWVNNGVCD SALNKPNRAS LRQLYDVAPI 2000
VLDNKYTVLQ DNTSQLIEPN VPVVEDVSIT TRKLIEVKCK GLNKPFVKGN 2050
FSFVNDPNGV TVVDTLGLTE LRALYVDINT RYIVLRDNNW SSLFKLHTVE 2100
SGDLQIVANG GSVTRRARVL LGASSLFASF AKITVTATTA ACKTAGRSFC 2150
KFVVNYGVLQ NMFLFLKMLF FLPFNYLWPK KQPTVDVGVS GLRTAGVVTT 2200
NIVKQCGTAA YYMLLGKFKR VDWKATLRLF LLLCTTILLL SSIYHLVIFN 2250
QVLSSDVMLE DATGILAMYK EVRSYLGIRT LCDGLAVEYR NTSFDVVDFC 2300
SNRSVLCQWC LIGQDSLTRY SALQMLQTHI TSYVLNIDWI WFALEFFLAY 2350
VLYTSSFNVL LLVVTAQYFF AYTSAFVNWR AYNYIVSGLF FLVTHIPLHG 2400
LVRVYNFLAC LWFLRKFYSH VINGCKDTAC LLCYKRNRLT RVEASTIVCG 2450
TKRTFYIAAN GGTSYCCKHN WNCVECDTAG VGNTFICTEV ANDLTTTLRR 2500
LIKPTDQSHY YVDSVVVKDA VVELHYNRDG SSCYERYPLC YFTNLEKLKF 2550
KEVCKTPTGI PEHNFLIYDT NDRGQENLAR SACVYYSQVL CKPMLLVDVN 2600
LVTTVGDSRE IAIKMLDSFI NSFISLFSVS RDKLEKLINT ARDCVRRGDD 2650
FQTVLKTFTD AARGHAGVES DVETTMVVDA LQYAHKNDIQ LTTECYNNYV 2700
PGYIKPDSIN TLDLGCLIDL KAASVNQTSM RNANGACVWN SGDYMKLSDS 2750
FKRQIRIACR KCNIPFRLTT SKLRAADNIL SVKFSATKIV GGAPSWLLRV 2800
RDLTVKGYCI LTLFVFTVAV LSWFCLPSYS IATVNFNDDR ILTYKVIENG 2850
IVRDIAPNDA CFANKYGHFS KWFNENHGGV YRNSVDCPIT IAVIAGVAGA 2900
RVANVPATLA WVGRQIVLFV SRVFANTNVC FTPTNEIPYD TFSDSGCVLS 2950
SECTLFRDAE GNLNPFCYDP TVLPGASSYA DMKPHVRYDM YDSDMYIKFP 3000
EVIFESTLRI TKTLATQYCR FGSCEESAAG VCISTNGSWA LYNQNYSTRP 3050
GIYCGDDYFD IVRRLAVSLF QPVTYFQLST SLAMGLVLCV FLTAAFYYIN 3100
KVKRALADYT QCAVVAVVAA LLNSLCLCFI VANPLLVAPY TAMYYYATFY 3150
LTGEPAFIMH ISWYVMFGTV VPIWMLASYT VGVMLRHLFW VLAYFSKKHV 3200
DVFTDGKLNC SFQDAASNIF VIGKDTYVAL RNAITQDSFV RYLSLFNKYK 3250
YYSGAMDTAS YREACAAHLC KALQTYSETG SDILYQPPNC SVTSSVLQSG 3300
LVKMSAPSGA VENCIVQVTC GSMTLNGLWL DNTVWCPRHI MCPADQLTDP 3350
NYDALLISKT NHSFIVQKHI GAQANLRVVA HSMVGVLLKL TVDVANPSTP 3400
AYTFSTVKPG ASFSVLACYN GKPTGVFTVN LRHNSTIKGS FLCGSCGSVG 3450
YTENGGVLNF VYMHQMELSN GTHTGSSFDG VMYGAFEDKQ THQLQLTDKY 3500
CTINVVAWLY AAVLNGCKWF VKPTRVGIVT YNEWALSNQF TEFVGTQSID 3550
MLAHRTGVSV EQMLAAIQSL HAGFQGKTIL GQSTLEDEFT PDDVNMQVMG 3600
VVMQSGVKRI SYGFMHWLMS TLVLAYVSVM QLTKFTMWTY LFETIPTQMT 3650
PLLFGFMACV MFTVKHKHTF LSLFLLPVAL CLTYANIVYE PQTLVSSTLI 3700
AVANWLTPTS VYMRTTHLDF GLYISLSFVL AIIVRRLYRP SMSNLALALC 3750
SGVMWFYTYV IGDHSSPITY LMFITTLTSD YTITVFATVN LAKFISGLVF 3800
LYAPHLGFIL PEVKLVLLIY LCLGYMCTMY FGVFSLLNLK LRVPLGVYDY 3850
SVSTQEFRFL TGNGLHAPRN SWEALILNFK LLGIGGTPCI KVATVQSKLT 3900
DLKCTSVVLL TVLQQLHLES NSKAWSYCVK LHNEILAAVD PTEAFERFVC 3950
LFATLMSFSA NVDLDALAND LFENSSVLQA TLTEFSHLAT YAELETAQSS 4000
YQKALNSGDA SPQVLKALQK AVNVAKNAYE KDKAVARKLE RMAEQAMTSM 4050
YKQARAEDKK AKIVSAMQTM LFGMIKKLDN DVLNGVIANA RNGCVPLSIV 4100
PLCASNKLRV VIPDISVWNK VVNWPSVSYA GSLWDVTVIN NVDNEVVKPT 4150
DVVETNESLT WPLVIECSRA SSSAVKLQNN EIHPKGLKTM VVTAGIDQVN 4200
CSSSAVAYYE PVQGHRMVMG LLSENAHLKW AKVEGKDGFI NIELQPPCKF 4250
LIAGPKGPEI RYLYFVKNLN NLHRGQLLGH IAATVRLQAG ANTEFASNST 4300
VLTLVAFAVD PAKAYLDYVG SGGTPLSNYV KMLAPKTGTG VAISVKPEAT 4350
ADQETYGGAS VCLYCRAHIE HPDVSGVCKY KTRFVQIPAH VRDPVGFLLK 4400
NVPCNVCQYW VGYGCNCDAL RNNTVPQSKD TNFLNRVRGS SVNARLEPCS 4450
SGLTTDVVYR AFDICNFKAR VAGIGKYYKT NTCRFVQVDD EGHKLDSYFI 4500
VKRHTMSNYE LEKRCYDLLK DCDAVAIHDF FIFDVDKTKT PHIVRQSLTE 4550
YTMMDLVYAL RHFDQNNCEV LKSILVKYGC CEQSYFDNKL WFDFVENPSV 4600
IGVYHKLGER IRQAMLNTVK MCDHMVKSGL VGVLTLDNQD LNGKWYDFGD 4650
FVITQPGAGV AIVDSYYSYL MPVLSMTNCL AAETHKDCDF NKPLIEWLLL 4700
EYDYTDYKIG LFNKYFKHWD QTYHPNCVNC GDDRCILHCA NFNVLFSMVL 4750
PNTSFGPIVR KIFVDGVPFI VSCGYHYKEL GLVMNMDVNI HRHRLALKEL 4800
MMYAADPAMH IASASALWDL RTPCFSVAAL TTGLTFQTVR PGNFNKDFYD 4850
FVVSRGFFKE GSSVTLKHFF FAQDGHAAIT DYSYYAYNLP TMVDIKQMLF 4900
CMEVVDKYFD IYDGGCLNAS EVIVNNLDKS AGHPFNKFGK ARVYYESMSY 4950
QEQDELFAVT KRNVLPTITQ MNLKYAISAK NRARTVAGVS ILSTMTNRQY 5000
HQKMLKSMAA TRGATCVIGT TKFYGGWDFM LKTLYKDVES PHLMGWDYPK 5050
CDRAMPNMCR ILASLILARK HSTCCTNSDR FYRLANECAQ VLSEYVLCGG 5100
GYYVKPGGTS SGDATTAYAN SVFNILQATT ANVSALMSAN GNTIIDREIK 5150
DMQFDLYINV YRKVVPDPKF VDKYYAFLNK HFSMMILSDD GVVCYNSDYA 5200
AKGYVASIQN FKETLYYQNN VFMSEAKCWV ETNLEKGPHE FCSQHTLYIK 5250
DGDDGYFLPY PDPSRILSAG CFVDDIVKTD GTVMMERYVS LAIDAYPLTK 5300
HDDTEYQNVF WVYLQYIEKL YKDLTGHMLD SYSVMLCGDD SAKFWEEGFY 5350
RDLYSSPTTL QAVGSCVVCH SQTSLRCGTC IRRPFLCCKC CYDHVIATTH 5400
KMVLSVSPYV CNAPGCDVSD VTKLYLGGMS YYCNDHRPVC SFPLCANGLV 5450
FGLYKNMCTG SSSIMEFNRL ATCDWSDSGD YTLANTTTEP LKLFAAETLR 5500
ATEEASKQSY AIATIKEIVG ERELILVWEV GKSKPPLNRN YVFTGYHLTK 5550
NSKVQLGEYV FERIDYSDAV SYKSSTTYKL AVGDIFVLTS HSVATLSAPT 5600
IVNQERYLKI TGIYPTITVP EEFANHVVNF QKAGFSKYVT VQGPPGTGKS 5650
HFAIGLAIYY PTARIVYTAC SHAAVDALCA KAFKYLNIAK CSRIIPAKAR 5700
VECYDRFKVN DTNAQYLFST VNALPEISVD ILVVDEVSMC TNYDLSIINS 5750
RVKAKHIVYV GDPAQLPAPR TLLTRGTLEP ENFNSVTRLM CNLGPDIFLS 5800
VCYRCPKEIV NTVSALVYNN KLSAKKDASG QCFKILFKGS VTHDASSAIN 5850
RPQLNFVKTF IAANPNWSKA VFISPYNSQN AVARSMLGLT TQTVDSSQGS 5900
EYPYVIFCQT ADTAHANNLN RFNVAVTRAQ KGILCVMTSQ VLFDSLEFAE 5950
LSLNNYKLQS QIVTGLFKDC SREDVGLPPA YAPTYLSVDA KYKTTDELCV 6000
NLNITPNVTY SRVISRMGFK LDATIPGYPK LFITRDEAIR QVRSWIGFDV 6050
EGAHASRNAC GTNVPLQLGF STGVNFVVQP VGVVDTEWGS MLTTISARPP 6100
PGEQFKHLVP LMNKGATWPI VRRRIVQMLS DTLDKLSDYC TFVCWAHGFE 6150
LTSASYFCKI GKEQRCCMCS RRASTFSSPL QSYACWSHSS GYDYVYNPFF 6200
VDVQQWGYVG NLATNHDRYC GIHAGAHVAS SDAIMTRCLA IYDCFIERVD 6250
WDVTYPYISH EQKLNSCCRT VERNVVRSAV LSGKFDKIYD IGNPKGIPII 6300
SEPVEWHFYD AQPLSNKVKK LFYTDDVAKQ FEDGLCLFWN CNVSKYPSNA 6350
VVCRFDTRVH SEFNLPGCNG GSLYVNKHAF HTPAYDINAF RDLKPLPFFY 6400
YSTTPCEVHG SGNMLEDIDY VPLKSAVCIT ACNLGGAVCR KHAAEYRDYM 6450
EAYNIVSAAG FRLWVYKTFD IYNLWSTFVK VQGLENIAFN VIKQGHFTGV 6500
DGELPVAVVN DKIFTKNGTD DVCIFKNETA LPTNVAFELY AKRAVRSHPD 6550
LNLLRNLEVD VCYNFVLWDY DRNNIYGTTT IGVCKYTDID VNPNLNMCFD 6600
IRDKGSLERF MSMPNGVLIS DRKIKNYPCI IGPKHAYFNG AILRNIDAKQ 6650
PITFYLYKKV NNEFVSFSDT FYTCGRTVND FTALTPMEED FLVLDSDVFI 6700
KKYSLEDYAF EHVVYGDFSH TTLGGLHLLI GLYKKMRDGH ILMEEMLKDR 6750
ATVHNYFITD SNTASYKAVC SVIDLRLDDF VNIIKEMDLD VVSKVVKVPI 6800
DLTMIEFMLW CKDGKVQTFY PRLQATNDWK PGLTMPSLFK VQQMNLEPCL 6850
LANYKQSIPM PNGVHMNVAK YMQLCQYLNT CTLAVPANMR VIHFGAGCEK 6900
GVAPGTSVLR QWLPLDAVLI DNDLNEFVSD ADITIFGDCV TVHVGQQVDL 6950
LISDMYDPCT KAVGEVNQTK ALFFVYLCNF IKNNLALGGS VAIKITEHSW 7000
SADLYKIMGR FAYWTVFCTN ANASSSEGFL IGINFLGELK EEIDGNVMHA 7050
NYIFWRNSTP MNLSTYSLFD LSRFPLKLKG TPVLQLKESQ INELVISLLS 7100
QGKLLIRDND TLNVSTDVLV NFRKRL 7126

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,126
Mass (Da):795,781
Last modified:June 10, 2008 - v1
Checksum:i23A0EB77D0CFD96F
GO
Isoform Replicase polyprotein 1a (identifier: P0C6F7-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6F7.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,441
Mass (Da):492,464
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648794 Genomic RNA. Translation: ABG47051.1.
RefSeqiYP_729202.1. NC_008315.1. [P0C6W1-1]

Genome annotation databases

GeneIDi5142284.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648794 Genomic RNA. Translation: ABG47051.1 .
RefSeqi YP_729202.1. NC_008315.1. [P0C6W1-1 ]

3D structure databases

ProteinModelPortali P0C6W1.
SMRi P0C6W1. Positions 3896-3979, 4228-4288, 4298-4418.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0C6W1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5142284.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses."
    Tohya Y., Narayanan K., Kamitani W., Huang C., Lokugamage K., Makino S.
    J. Virol. 83:5282-5288(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF NSP1.

Entry informationi

Entry nameiR1AB_BC133
AccessioniPrimary (citable) accession number: P0C6W1
Secondary accession number(s): Q0Q4F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi