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Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).By similarity
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1103For PL1-PRO activityPROSITE-ProRule annotation1
Active sitei1252For PL1-PRO activityPROSITE-ProRule annotation1
Active sitei1737For PL2-PRO activityPROSITE-ProRule annotation1
Active sitei1902For PL2-PRO activityPROSITE-ProRule annotation1
Active sitei3053For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3156For 3CL-PRO activityPROSITE-ProRule annotation1
Metal bindingi5043Zinc 1PROSITE-ProRule annotation1
Metal bindingi5046Zinc 1PROSITE-ProRule annotation1
Metal bindingi5054Zinc 2PROSITE-ProRule annotation1
Metal bindingi5057Zinc 1PROSITE-ProRule annotation1
Metal bindingi5064Zinc 1PROSITE-ProRule annotation1
Metal bindingi5067Zinc 2PROSITE-ProRule annotation1
Metal bindingi5071Zinc 2PROSITE-ProRule annotation1
Metal bindingi5077Zinc 2PROSITE-ProRule annotation1
Metal bindingi5088Zinc 3PROSITE-ProRule annotation1
Metal bindingi5093Zinc 3PROSITE-ProRule annotation1
Metal bindingi5110Zinc 3PROSITE-ProRule annotation1
Metal bindingi5113Zinc 3PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1174 – 1205C4-typePROSITE-ProRule annotationAdd BLAST32
Zinc fingeri4050 – 4066By similarityAdd BLAST17
Zinc fingeri4092 – 4105By similarityAdd BLAST14
Nucleotide bindingi5321 – 5328ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Methyltransferase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p9
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p87
Alternative name(s):
PL1-PRO/PL2-PRO
PLP1/PLP2
Papain-like proteinases 1/2
p195
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p34
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p5
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p23
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p14
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
p100
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
p66
p66-HEL
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
Taxonomic identifieri693999 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiScotophilus kuhlii (Lesser asiatic yellow bat) [TaxID: 153297]

Subcellular locationi

Non-structural protein 7 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 8 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 9 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 10 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Helicase :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1973 – 1993HelicalSequence analysisAdd BLAST21
Transmembranei2036 – 2056HelicalSequence analysisAdd BLAST21
Transmembranei2119 – 2139HelicalSequence analysisAdd BLAST21
Transmembranei2141 – 2161HelicalSequence analysisAdd BLAST21
Transmembranei2164 – 2184HelicalSequence analysisAdd BLAST21
Transmembranei2543 – 2563HelicalSequence analysisAdd BLAST21
Transmembranei2634 – 2654HelicalSequence analysisAdd BLAST21
Transmembranei2669 – 2689HelicalSequence analysisAdd BLAST21
Transmembranei2769 – 2789HelicalSequence analysisAdd BLAST21
Transmembranei2802 – 2822HelicalSequence analysisAdd BLAST21
Transmembranei2829 – 2849HelicalSequence analysisAdd BLAST21
Transmembranei2878 – 2898HelicalSequence analysisAdd BLAST21
Transmembranei3351 – 3371HelicalSequence analysisAdd BLAST21
Transmembranei3376 – 3396HelicalSequence analysisAdd BLAST21
Transmembranei3414 – 3434HelicalSequence analysisAdd BLAST21
Transmembranei3443 – 3463HelicalSequence analysisAdd BLAST21
Transmembranei3466 – 3486HelicalSequence analysisAdd BLAST21
Transmembranei3488 – 3507HelicalSequence analysisAdd BLAST20
Transmembranei3511 – 3531HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002899111 – 110Non-structural protein 1By similarityAdd BLAST110
ChainiPRO_0000289912111 – 897Non-structural protein 2By similarityAdd BLAST787
ChainiPRO_0000289913898 – 2530Non-structural protein 3By similarityAdd BLAST1633
ChainiPRO_00002899142531 – 3012Non-structural protein 4By similarityAdd BLAST482
ChainiPRO_00002899153013 – 33143C-like proteinaseBy similarityAdd BLAST302
ChainiPRO_00002899163315 – 3590Non-structural protein 6By similarityAdd BLAST276
ChainiPRO_00002899173591 – 3673Non-structural protein 7By similarityAdd BLAST83
ChainiPRO_00002899183674 – 3868Non-structural protein 8By similarityAdd BLAST195
ChainiPRO_00002899193869 – 3976Non-structural protein 9By similarityAdd BLAST108
ChainiPRO_00002899203977 – 4111Non-structural protein 10By similarityAdd BLAST135
ChainiPRO_00002899214112 – 5038RNA-directed RNA polymeraseBy similarityAdd BLAST927
ChainiPRO_00002899225039 – 5557HelicaseBy similarityAdd BLAST519
ChainiPRO_00002899235558 – 6153ExoribonucleaseBy similarityAdd BLAST596
ChainiPRO_00002899246154 – 6492Uridylate-specific endoribonucleaseBy similarityAdd BLAST339
ChainiPRO_00002899256493 – 6793Putative 2'-O-methyl transferaseBy similarityAdd BLAST301

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110 – 111Cleavage; by PL1-PROBy similarity2
Sitei897 – 898Cleavage; by PL1-PROBy similarity2
Sitei2530 – 2531Cleavage; by PL2-PROBy similarity2
Sitei3012 – 3013Cleavage; by 3CL-PROBy similarity2
Sitei3314 – 3315Cleavage; by 3CL-PROBy similarity2
Sitei3590 – 3591Cleavage; by 3CL-PROBy similarity2
Sitei3673 – 3674Cleavage; by 3CL-PROBy similarity2
Sitei3868 – 3869Cleavage; by 3CL-PROBy similarity2
Sitei3976 – 3977Cleavage; by 3CL-PROBy similarity2
Sitei4111 – 4112Cleavage; by 3CL-PROBy similarity2
Sitei5038 – 5039Cleavage; by 3CL-PROBy similarity2
Sitei5557 – 5558Cleavage; by 3CL-PROBy similarity2
Sitei6153 – 6154Cleavage; by 3CL-PROBy similarity2
Sitei6492 – 6493Cleavage; by 3CL-PROBy similarity2

Proteomic databases

PRIDEiP0C6W0.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6W0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1069 – 1302Peptidase C16 1PROSITE-ProRule annotationAdd BLAST234
Domaini1303 – 1467MacroPROSITE-ProRule annotationAdd BLAST165
Domaini1699 – 1965Peptidase C16 2PROSITE-ProRule annotationAdd BLAST267
Domaini3013 – 3314Peptidase C30PROSITE-ProRule annotationAdd BLAST302
Domaini5039 – 5122CV ZBDPROSITE-ProRule annotationAdd BLAST84
Domaini5296 – 5477(+)RNA virus helicase ATP-bindingAdd BLAST182
Domaini5478 – 5647(+)RNA virus helicase C-terminalAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1973 – 2184HD1By similarityAdd BLAST212
Regioni2543 – 2898HD2By similarityAdd BLAST356
Regioni3351 – 3531HD3By similarityAdd BLAST181
Regioni4649 – 4951RNA-directed RNA polymeraseBy similarityAdd BLAST303

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1827 – 1946Val-richAdd BLAST120

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 CV ZBD (coronavirus zinc-binding) domain.PROSITE-ProRule annotation
Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1174 – 1205C4-typePROSITE-ProRule annotationAdd BLAST32
Zinc fingeri4050 – 4066By similarityAdd BLAST17
Zinc fingeri4092 – 4105By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR032505. Corona_NSP4_C.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_ZBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Replicase polyprotein 1ab (identifier: P0C6W0-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MASNHISLAF ANDEEISAIG FGSVEEAVSY YSDAAVNGFD QCRFVSLGLQ
60 70 80 90 100
DAVVGVEDDD VVMLITGVTQ LRAYLGTFGD RPLNLRGWLL FSNCNYFLEE
110 120 130 140 150
LDLVFGRCGG TTIPVDQFMC GADGAPVIQE GDWTFMDYFQ DSNQFTLNGI
160 170 180 190 200
TYVKAWDVDR KPNDYAKQNV TCIRRITYIT DHRHVLADGT TMKTARHPKV
210 220 230 240 250
NKSVVLDSPF DQIYKEVGSP FMGNGSTFVE MLKDPAFFHA LITCECGRSE
260 270 280 290 300
WTVGDWKGYN SLCCNIKCKP ITIVTPKAVP GAVVITKAGI GAGLKCYNNV
310 320 330 340 350
FLKHIIDLVV PGTNLGWGVW RIAKVQSKDD VATSGNVLVD DPEDRLDPCY
360 370 380 390 400
FGNDGPFATK FKFQLLANSF DDEVKGAIVQ GVVHVNTAIC DVVKDILGLP
410 420 430 440 450
WFVKKLGSLV TVMWDQFVAG VQSMKICTLK VVQLAKALSC ATMSVVKGVI
460 470 480 490 500
TLVAEVPEIF KRLFYTLTSA LKSLCTSSCD ALVVAGKSFA KIGDYVLLPS
510 520 530 540 550
ALVRLVSSKV KGKAQSGIKQ LQFATVVLGD THKVESDRVE FSSVNLKMVD
560 570 580 590 600
EEFPLNPVGH TVAVGNQAFF CSDGLYRFMA DRDLVITSPI FKPELELEPI
610 620 630 640 650
FECDAIPGFP KVAASNVAEL CVKVDTLLFN YDKIYKKYST IIKGDRCYIQ
660 670 680 690 700
CTHTFKAPSY YFDDDEFVEL CTKYYKLPDF DAFYNAVHAA TDMDQFCALC
710 720 730 740 750
TSGFEVFIPR VPDCPPILND IDGGSIWTSF ILSVRSATDF IKTLKIDLGL
760 770 780 790 800
NGVVVFVTKK FRKAGALLQK LYNAFLDTVT SFIKVAGVAF KYCATCVPKI
810 820 830 840 850
VINGCYHTVT RLFAKDLQIP TEDGVADFNT FNHCVFPVNP TRIETDSLEL
860 870 880 890 900
EEVDFVEPGV DGKLVILDDY SFYSDGTNYY PSDGKGVVAS CFKKKGGGVV
910 920 930 940 950
TISDEVQVRT IDPVYKVRLE YEFEDETLVK VCEKAIGTKL KVTGDWSNLL
960 970 980 990 1000
ETLEKAMDVV RQHLDVPDYF VYDEEGGTDL NLTIMVSQWP LSSDSEDDFK
1010 1020 1030 1040 1050
AVDDEPNANT DETVDTFAED VAETQNVQQD VTQDEVEAVC DLVVKATEEG
1060 1070 1080 1090 1100
PIEHEELSED QKEVQQALAF IEDKPVVVKP DVFAFSYASY GGLKVLNQSS
1110 1120 1130 1140 1150
NNCWVSSALV QLQLTGLLDS DEMQLFNAGR VSPMVKRCYE SQRAIFGSLG
1160 1170 1180 1190 1200
DVSACLESLL KDRDGMSITC TIDCGCGPGV RVYENAIFRF TPLKTAFPMG
1210 1220 1230 1240 1250
RCLICSKTLM HTITQMKGTG IFCRDATALD VDTLVVKPLC AAVYVGAQDG
1260 1270 1280 1290 1300
GHYLTNMYDA NMAVDGHGRH PIKFNTINTL CYKDVDWEVS NGSCDVKPFL
1310 1320 1330 1340 1350
TYKNIEFYQG ELSALLSVNH DFVVNAANEQ LSHGGGIAKA LDDLTKGELQ
1360 1370 1380 1390 1400
VLSNQYVSRN GSIKVGSGVL IKCKEHSILN VVGPRKGKHA AELLTKAYTF
1410 1420 1430 1440 1450
VFKQKGVPLM PLLSVGIFKV PITESLAAFL ACVGDRVCKC FCYTDKERLA
1460 1470 1480 1490 1500
IQNFVTSFQT EQPVEPLPVI QEVKGVQLEK PVPDVKVENP CEPFRIEGDA
1510 1520 1530 1540 1550
KFYDLTPSMV QSLQVTRLVS FTNSDLCLGS FVRDCDGYVQ GSLGGAIANY
1560 1570 1580 1590 1600
KKSNPVLPAG NCVTLKCDGF ISFTFVILPK EGDTNYEKNF NRAIAKFLKL
1610 1620 1630 1640 1650
KGSLLVVVED SSVFNKISHA SVAGYVAKPA LVDTLFEAKP VQVVVTQDQR
1660 1670 1680 1690 1700
SFHTVELSTS QTYGQQLGDC VVEDKKVTNL KPVSKDKVVS VVPNVDWDKH
1710 1720 1730 1740 1750
YGFVDAGIFH TLDHTMFVFD NNVVNGKRVL RTSDNNCWIN AVCLQLQFAN
1760 1770 1780 1790 1800
AKFKPKGLQQ LWESYCTGDV AMFVHWLYWI TGVEKGEPSD AENTLNIISR
1810 1820 1830 1840 1850
FLKPQGSVEM LRATSTTCDG TCSTKRVVST PVVNASVLKV GLDDGNCVHG
1860 1870 1880 1890 1900
LPLVDRVVSV NGTVIITNVG DTPGKPVVAT ENLLLDGVSY TVFQDSTTGV
1910 1920 1930 1940 1950
GHYTVFDKEA KLMFDGDVLK PCDLNVSPVT SVVVCNNKKI VVQDPVKRVE
1960 1970 1980 1990 2000
LDASKFLDTM NVASEKFFTF GDFVSRNIIV LIVYLFSLLA ICFRALKKRD
2010 2020 2030 2040 2050
MKVMAGVPER TGIILKRSVK YNYKALKFFF RLKFQYIKVF LKFSLVLYTL
2060 2070 2080 2090 2100
YALMFMFIRF TPVGTPICKR YTDGYANSTF DKNDYCGNVL CKICLYGYEE
2110 2120 2130 2140 2150
LSDFTHTRVI WQHLKDPLIG NILPLFYLVF LIIFGGFFVR IGITYFIMQY
2160 2170 2180 2190 2200
INAAGVALGY QDNVWLLHLL PFNSMGNIIV VAFIVTRILL FLKHVLFGCD
2210 2220 2230 2240 2250
KPSCIACSKS AKLTRVPLQT ILQGVTKSFY VNANGGKKFC KKHNFFCVDC
2260 2270 2280 2290 2300
DSYGYGCTFI NDVIAPELSN VTKLNVIPTG PATIIIDKVE FSNGFYYLYS
2310 2320 2330 2340 2350
GSTFWKYNFD ITEAKYACKD VLKNCNILTD FVVFNNSGSN VTQVKNACVY
2360 2370 2380 2390 2400
FSQLLCKPIK LVDSALLASL NVDFSANLHK AFVEVLSNSF GKDLSNCSNM
2410 2420 2430 2440 2450
NECRESLGLS DVPEEEFSAA VSEAHRYDVL ISDVSFNNLI VSYAKPEEKL
2460 2470 2480 2490 2500
AVHDIANCMR VGAKVVNHNV LTKDNVPVVW LAKDFIALSE EARKYIVRTT
2510 2520 2530 2540 2550
KTKGINFMLT FNDRRMHLTI PTISVANKKG AGLPSLFTRL YSFFWHLCVL
2560 2570 2580 2590 2600
IVVLFVATSL LDFSAQVTSD TQYDFKYIEN GVLKVFEKPL DCVHNAFVNF
2610 2620 2630 2640 2650
NEWHNAKFGS IPTNSRRCPI VVGTSDEVRY IPGVPAGVFL YGKSLIFAMS
2660 2670 2680 2690 2700
TIFGTSGLCF DDRGLTDPDS CIFNSACTTL SGIGGRNVYC YREGVVDNAK
2710 2720 2730 2740 2750
LYSSLLPHSY YRLMDGNHIV LPEIITRGFG IRTIKTQAMT YCRTGECIDS
2760 2770 2780 2790 2800
QAGVCVGLDR FFVYSKTPGS DYVCGTGFFS LLFNVIGMFS NSIPVTVMSG
2810 2820 2830 2840 2850
QILLNCVVAF TAVMACFAFT KFKRLFGDMS FGVLSVGLCT VVNNLSYVVT
2860 2870 2880 2890 2900
QNSIGMLAYA TLYFLCTKGV RYSWVWHVGF AISYCFLAPW WVVLAYLICA
2910 2920 2930 2940 2950
LLEFLPNLFK LKVSTQLFEG DKFVGSFESA ASGTFVLDMH SYQKLANSIS
2960 2970 2980 2990 3000
TEKLKQYCAS YNRYKYYSGS ASEADYRLAC FAHLAKAMSD FANDHMDKLY
3010 3020 3030 3040 3050
TPPTVSYNST LQAGLRKMAQ PSGIVEGCIV RVSYGNLTLN GLWLGDTVIC
3060 3070 3080 3090 3100
PRHVIASNTT NVIDYDHAMS LVRLHNFSIS SGNMFLGVIS ASMRGTLLHI
3110 3120 3130 3140 3150
KVNQSNVNTP NYTYKVLKPG DSFNILACYD GSAAGVYGVN MRTNYTIRGS
3160 3170 3180 3190 3200
FISGACGSPG YNINNGVVEF CYMHHLELGS GCHVGSDMDG TMYGKYEDQP
3210 3220 3230 3240 3250
TLQIEGASNL VTENVCSWLY GALINGDRWW LSSVSVGVDT YNEWALRNGM
3260 3270 3280 3290 3300
TALKNVDCFS LLVAKTGVDV GRLLASIQKL HGNFGGKSIL GCTSLCDEFT
3310 3320 3330 3340 3350
LSEVVKQMYG VTLQSGKVSR AFRNASIVCC LLFLFLSEML NHSKLFWINP
3360 3370 3380 3390 3400
GYITPVFLAI IVASSALMLL VKHKLLFLQL YLLPSLCIVS GYNIFKDYHF
3410 3420 3430 3440 3450
YTYMLEEFDY KVPFGGFNVT GVLNISLCCF VMGLHTFRFL QTPNKIFSYV
3460 3470 3480 3490 3500
VAVLTVLYTY YYSTDVLGLI LTSMSGFTNY WFIGTATYKL ATYVLPHTSL
3510 3520 3530 3540 3550
LDSFDAIKAV VFLYLLLGYC NCVYYGSLYW INRFCKLTLG CYEFKVSAAE
3560 3570 3580 3590 3600
FKYMVANGLR APTGVFDALI LSLKLIGVGG RKTIKISSVQ SKLTDLKCTN
3610 3620 3630 3640 3650
VVLLGCLSNM NIAANSREWA YCVDLHNKIN LCNDAEAAQE MLLALLAFFL
3660 3670 3680 3690 3700
SKNSAFGVDE LLDSYFNDSS VLQSVAATYV NLPSYLAYET ARQSYEDALA
3710 3720 3730 3740 3750
NGSPPQLVKQ LRHAMNVAKS EFDREASTQR KLDRMAEQAA SQMYKEARAV
3760 3770 3780 3790 3800
NRKSKVVSAM HSLLFGMLRR LDMSSVDTIL SLAKDGVVPL SIIPAVSATK
3810 3820 3830 3840 3850
LNIVVSDIES YSKIQREGCV HYAGVIWSVV DIKDNDGKPV HAKEVVTSNV
3860 3870 3880 3890 3900
ESLAWPLFLN CERIIKLQNN EIIPSKIKQR PIKAEGEGVV ADGNALYSNE
3910 3920 3930 3940 3950
GGRTFMYAFI SDKPDLKVVK WEFDGGSNAI ELEPPCKFLV EAPSGPVVKY
3960 3970 3980 3990 4000
LYFVRNLNNL RRGAVLGFIG ATVRLQAGKQ TEQATNSSLL TLCAFAVDPP
4010 4020 4030 4040 4050
KTYLDAVKSG HRPVGNCVKM LANGSGNGQA ITNGVEASTN QDSYGGASVC
4060 4070 4080 4090 4100
LYCRAHVEHP DMDGFCKLRG KYVQVPLGTL DPIRFVLENT VCKVCGCWQA
4110 4120 4130 4140 4150
NGCTCDRAVI QSVDSGYLNR VRGSSAARLE PLNGSDTHHV FRAFDVYNRD
4160 4170 4180 4190 4200
VACISKFLKV NCVRLKNLDK HDAFWIVKKC TKSVMEHEQS IYNLISDCGA
4210 4220 4230 4240 4250
VAKHDFFTWK EGRSVYGNVC RQDLTEYTMM DLCYALRNFD ENNCETLKKI
4260 4270 4280 4290 4300
LVVVGACDES YFDNKLWFDP VENEDVHRVY AKLGTIVARA MLKCVKYCDA
4310 4320 4330 4340 4350
MVEQGIVGVI TLDNQDLNGD FYDFGDFVTS VKGMGVPICT SYYSYMMPVM
4360 4370 4380 4390 4400
GMTNCLASEC FIKSDIFGED FRTFDLLAYD FTEHKVNLFN KYFKHWGQTY
4410 4420 4430 4440 4450
HPNCEDCHDE SCIVHCANFN TLFATTIPIT AFGPLCRKCW IDGVPLVTTA
4460 4470 4480 4490 4500
GYHFKQLGIV WNKDLNLHSS RLTINELLQF CADPSLLIAS SPALVDKRTV
4510 4520 4530 4540 4550
CFSVAALGTG MTNQTVKPGH FNREFYDFLR SQGFFEEGSE LTLKHFFFAQ
4560 4570 4580 4590 4600
KGDAAVRDFD YYRYNRTTVL DICQARVVYQ IVQCYFGMYE GGCITAKEVI
4610 4620 4630 4640 4650
VNNLNKSAGY PFNKFGKAGL YYDSLSYEEQ DDLYAYTKRN IIPTMTQLNL
4660 4670 4680 4690 4700
KYAISGKDRA RTVGGVSLLS TMTTRQYHQK HLKSIVNTRG ASVVIGTTKF
4710 4720 4730 4740 4750
YGGWDNMLKT LIKDVENPHL MGWDYPKCDR ALPNMIRMIS AMILGSKHVN
4760 4770 4780 4790 4800
CCSSSDRYYR LCNELAQVLT EMVYSNGGFY VKPGGTTSGD ATTAYANSVF
4810 4820 4830 4840 4850
NIFQATSANV NRLLSVDSNT CNNIEVKQLQ RKLYDCCYRS SSVDQSFVEE
4860 4870 4880 4890 4900
YFGYLRKHFS MMILSDDGVV CYNSEYAALG YVADLNAFKA VLYYQNNVFM
4910 4920 4930 4940 4950
SASKCWIEPD INKGPHEFCS QHTMQIVDKD GTYYLPYPDP SRILSAGVFV
4960 4970 4980 4990 5000
DDIVKTDPVI LLERYVSLAI DAYPLSKHDN PEYRRVFTVM LDWVKHLYKT
5010 5020 5030 5040 5050
LNQGVLDSFS VTLLEDATAK FWDESFYASM YEQSSVLQSA GLCVVCSSQT
5060 5070 5080 5090 5100
VLRCGDCIRR PMLCTKCAYD HVVSTSHKFI LAITPYVCCS SGCGVSDVTK
5110 5120 5130 5140 5150
LYLGGLSYWC VDHKPRLSFP LCSSGNVFGL YKNSATGSPD VDDFNTLATS
5160 5170 5180 5190 5200
DWTDVKDYKL ANDVKDSLRL FAAETIKAKE ESVKSSYACA TIHEVVGPKE
5210 5220 5230 5240 5250
LVLKWEVGKP RPPLSRNSVF TCYHITKNTK FQVGEFTFEK LDYDNDAVSY
5260 5270 5280 5290 5300
KSTATTKLVP GMVFVLTSHN VQPLRAPTII NQERYSTLHK LRPAFNIHED
5310 5320 5330 5340 5350
YSNLIPYYQL IGKQKLTTIQ GPPGSGKSHC VIGLGLYFPG ARIVFTACSH
5360 5370 5380 5390 5400
AAVDSLCVKA ATAYSSDRCS RIIPQKARIE CYDGFKSNNT SAQYLFSTVN
5410 5420 5430 5440 5450
ALPEVNADIC VVDEVSMCTN YDLSVINQRV NYRHIVYVGD PQQLPAPRVM
5460 5470 5480 5490 5500
ITRGVLVPED YNVVTRRMCV LKPDIFLHKC YRCPAEIVNT VSEMVYENQF
5510 5520 5530 5540 5550
VPVKSESKEC FKIYCRGNVQ VDNGSSINRR QLEVVRMFLA KNPKWAKAVF
5560 5570 5580 5590 5600
ISPYNSQNYV AGRVLGLQIQ TVDSSQGSEY DYVIYTQTSD TAHASNVNRF
5610 5620 5630 5640 5650
NVAITRAKKG ILCIMCDREL FDILKFYELK LSDLQVGDGC GLFKDCYKGE
5660 5670 5680 5690 5700
DNLPPSHAPT FMSLSDNFKT DKDLAVQIGV NGPVKYEHVI SFMGFRFDIN
5710 5720 5730 5740 5750
VPNQHTLFCT RDFAMRNARG WLGFDVEGAH VIGSNVGTNV PLQLGFSNGV
5760 5770 5780 5790 5800
DFVVRPEGCV STEVGDVIQP VRARAPPGDQ FTHLLPLLRK GQPWSVIRRR
5810 5820 5830 5840 5850
IVQMCSDYLA NLSDTLIFVL WSGGLELTTM RYFVKLGPVQ TCDCGKRATC
5860 5870 5880 5890 5900
YNSTNHTFSC FRHALGSDYI YNCYCIDIQQ WGYTGSLSMN HHEVCNIHRN
5910 5920 5930 5940 5950
EHVASGDAAM TRCLAIHDCF VKNVDWSITY PFIANEQAIN KSGRLVQSHV
5960 5970 5980 5990 6000
MRAVLKLYNP KAIHDVGNPK GIRCVVTDAS WYCYDKNPTN TNVKMLEYDY
6010 6020 6030 6040 6050
ITHGQLDGLC LFWNCNVDMY PEFSVVCRFD TRMRSTLNLE GCNGGSLYVN
6060 6070 6080 6090 6100
NHAFHTPAYD KRAFAKLKAM PFFFYDDSEC EKLQDAVNYV PLRASNCITR
6110 6120 6130 6140 6150
CNVGGAVCSK HCALYHNYVM AYNTFTTAGF TIWVPNSFDM FNLWQTFKNS
6160 6170 6180 6190 6200
NVQGLENIAY NVVKKGSFVG VEGELPVAVV NDKVMVRDGV SDNVVFVNNT
6210 6220 6230 6240 6250
SLPTNVAFEL YAKRKVGLTP PLTILKNLGV VCTSKCVLWD YEASRPLTTF
6260 6270 6280 6290 6300
TKDVCKYTDF DGDVCTLFDN SVPGAFERFT VTKNAVLISL TAVKKLTAIK
6310 6320 6330 6340 6350
LTYGYLNGVP VFTHEDKPFT WYIYTRKDGA FVEYPDGYFT QGRVISDFQP
6360 6370 6380 6390 6400
RSNMEEDFLN MDMGLFISKY GLEDYGFEHV VFGDVSKTTL GGLHLLISQI
6410 6420 6430 6440 6450
RLSKIGVLKV EDFVSSSDST LKSCTVTYVD NPSSKMVCTY VDLLLDDFVN
6460 6470 6480 6490 6500
ILKSVDLSVV SKVHEVVIDC KVWRWMLWCK DHKVQTFYPQ LQSAEWKCGY
6510 6520 6530 6540 6550
SMPSIYKIQR MCLEPCNLYN YGSGLKLPDG IMFNVVKYTQ LCQYLNSTTM
6560 6570 6580 6590 6600
CVPHHMRVLH LGAGSDKGVA PGTAVLRRWL PLDAVIVDND VNDYVSDADF
6610 6620 6630 6640 6650
SYTGDCASMY LTDKFDLVIS DMYDGRTKSC DGDNVSKEGF FPYINGVITE
6660 6670 6680 6690 6700
KLALGGTVAI KITEFSWNKK LYELIQKFEY WTLFCTSVNT SSSEAFLIGV
6710 6720 6730 6740 6750
HFLGDFSTNA IIDGNIMHAN YIFWRNSTIM TMSYNSVLDL SKFSCKHKAT
6760 6770 6780 6790
VVVNLKDSSV TDLVLGLLKN GKLLIRNNGV VCGFSNHLVN STK
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Length:6,793
Mass (Da):757,262
Last modified:June 10, 2008 - v1
Checksum:i6C76171F3E67FE1D
GO
Isoform Replicase polyprotein 1a (identifier: P0C6F6-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
The sequence of this isoform can be found in the external entry P0C6F6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.
Length:4,128
Mass (Da):456,581
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ648858 Genomic RNA. Translation: ABG47077.1.
RefSeqiYP_001351683.1. NC_009657.1. [P0C6W0-1]

Genome annotation databases

GeneIDi11266518.
KEGGivg:11266518.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ648858 Genomic RNA. Translation: ABG47077.1.
RefSeqiYP_001351683.1. NC_009657.1. [P0C6W0-1]

3D structure databases

ProteinModelPortaliP0C6W0.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP0C6W0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11266518.
KEGGivg:11266518.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR032505. Corona_NSP4_C.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_ZBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiR1AB_BC512
AccessioniPrimary (citable) accession number: P0C6W0
Secondary accession number(s): Q0Q467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus 512/2005 is highly similar to porcine epidemic diarrhea virus (PEDV).

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.