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P0C6W0

- R1AB_BC512

UniProt

P0C6W0 - R1AB_BC512

Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
    The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.By similarity
    The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.By similarity
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    ATP + H2O = ADP + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei110 – 1112Cleavage; by PL1-PROBy similarity
    Sitei897 – 8982Cleavage; by PL1-PROBy similarity
    Active sitei1103 – 11031For PL1-PRO activityPROSITE-ProRule annotation
    Active sitei1252 – 12521For PL1-PRO activityPROSITE-ProRule annotation
    Active sitei1737 – 17371For PL2-PRO activityPROSITE-ProRule annotation
    Active sitei1902 – 19021For PL2-PRO activityPROSITE-ProRule annotation
    Sitei2530 – 25312Cleavage; by PL2-PROBy similarity
    Sitei3012 – 30132Cleavage; by 3CL-PROBy similarity
    Active sitei3053 – 30531For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3156 – 31561For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3314 – 33152Cleavage; by 3CL-PROBy similarity
    Sitei3590 – 35912Cleavage; by 3CL-PROBy similarity
    Sitei3673 – 36742Cleavage; by 3CL-PROBy similarity
    Sitei3868 – 38692Cleavage; by 3CL-PROBy similarity
    Sitei3976 – 39772Cleavage; by 3CL-PROBy similarity
    Sitei4111 – 41122Cleavage; by 3CL-PROBy similarity
    Sitei5038 – 50392Cleavage; by 3CL-PROBy similarity
    Sitei5557 – 55582Cleavage; by 3CL-PROBy similarity
    Sitei6153 – 61542Cleavage; by 3CL-PROBy similarity
    Sitei6492 – 64932Cleavage; by 3CL-PROBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1174 – 120532C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4050 – 406617By similarityAdd
    BLAST
    Zinc fingeri4092 – 410514By similarityAdd
    BLAST
    Nucleotide bindingi5321 – 53288ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    5. helicase activity Source: UniProtKB-KW
    6. methyltransferase activity Source: InterPro
    7. omega peptidase activity Source: InterPro
    8. RNA binding Source: UniProtKB-KW
    9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    10. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of host autophagy Source: UniProtKB-KW
    2. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    3. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    4. transcription, DNA-templated Source: InterPro
    5. viral protein processing Source: InterPro
    6. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Activation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1ab
    Short name:
    pp1ab
    Alternative name(s):
    ORF1ab polyprotein
    Cleaved into the following 15 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    p9
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p87
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PLP1/PLP2
    Papain-like proteinases 1/2
    p195
    Non-structural protein 4
    Short name:
    nsp4
    Alternative name(s):
    Peptide HD2
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    M-PRO
    nsp5
    p34
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Alternative name(s):
    p5
    Non-structural protein 8
    Short name:
    nsp8
    Alternative name(s):
    p23
    Non-structural protein 9
    Short name:
    nsp9
    Alternative name(s):
    p12
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    p14
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Short name:
    Pol
    Short name:
    RdRp
    Alternative name(s):
    nsp12
    p100
    Helicase (EC:3.6.4.12, EC:3.6.4.13)
    Short name:
    Hel
    Alternative name(s):
    nsp13
    p66
    p66-HEL
    Exoribonuclease (EC:3.1.13.-)
    Short name:
    ExoN
    Alternative name(s):
    nsp14
    Alternative name(s):
    NendoU
    nsp15
    Alternative name(s):
    nsp16
    Gene namesi
    Name:rep
    ORF Names:1a-1b
    OrganismiBat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
    Taxonomic identifieri693999 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
    Virus hostiScotophilus kuhlii (Lesser asiatic yellow bat) [TaxID: 153297]

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
    Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. host cell membrane Source: UniProtKB-SubCell
    3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 110110Non-structural protein 1By similarityPRO_0000289911Add
    BLAST
    Chaini111 – 897787Non-structural protein 2By similarityPRO_0000289912Add
    BLAST
    Chaini898 – 25301633Non-structural protein 3By similarityPRO_0000289913Add
    BLAST
    Chaini2531 – 3012482Non-structural protein 4By similarityPRO_0000289914Add
    BLAST
    Chaini3013 – 33143023C-like proteinaseBy similarityPRO_0000289915Add
    BLAST
    Chaini3315 – 3590276Non-structural protein 6By similarityPRO_0000289916Add
    BLAST
    Chaini3591 – 367383Non-structural protein 7By similarityPRO_0000289917Add
    BLAST
    Chaini3674 – 3868195Non-structural protein 8By similarityPRO_0000289918Add
    BLAST
    Chaini3869 – 3976108Non-structural protein 9By similarityPRO_0000289919Add
    BLAST
    Chaini3977 – 4111135Non-structural protein 10By similarityPRO_0000289920Add
    BLAST
    Chaini4112 – 5038927RNA-directed RNA polymeraseBy similarityPRO_0000289921Add
    BLAST
    Chaini5039 – 5557519HelicaseBy similarityPRO_0000289922Add
    BLAST
    Chaini5558 – 6153596ExoribonucleaseBy similarityPRO_0000289923Add
    BLAST
    Chaini6154 – 6492339Uridylate-specific endoribonucleaseBy similarityPRO_0000289924Add
    BLAST
    Chaini6493 – 6793301Putative 2'-O-methyl transferaseBy similarityPRO_0000289925Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Proteomic databases

    PRIDEiP0C6W0.

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C6W0.
    SMRiP0C6W0. Positions 3013-3311, 3871-3975, 3982-4107.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1973 – 199321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2036 – 205621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2119 – 213921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2141 – 216121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2164 – 218421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2543 – 256321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2634 – 265421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2669 – 268921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2769 – 278921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2802 – 282221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2829 – 284921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2878 – 289821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3351 – 337121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3376 – 339621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3414 – 343421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3443 – 346321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3466 – 348621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3488 – 350720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3511 – 353121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1069 – 1302234Peptidase C16 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1303 – 1467165MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1699 – 1965267Peptidase C16 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini3013 – 3314302Peptidase C30PROSITE-ProRule annotationAdd
    BLAST
    Domaini5039 – 512284CV MBDAdd
    BLAST
    Domaini5296 – 5477182(+)RNA virus helicase ATP-bindingAdd
    BLAST
    Domaini5478 – 5647170(+)RNA virus helicase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1973 – 2184212HD1By similarityAdd
    BLAST
    Regioni2543 – 2898356HD2By similarityAdd
    BLAST
    Regioni3351 – 3531181HD3By similarityAdd
    BLAST
    Regioni4649 – 4951303RNA-directed RNA polymeraseBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1827 – 1946120Val-richAdd
    BLAST

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1174 – 120532C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4050 – 406617By similarityAdd
    BLAST
    Zinc fingeri4092 – 410514By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR009466. NSP11.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF06478. Corona_RPol_N. 1 hit.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 2 hits.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 2 hits.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1ab (identifier: P0C6W0-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MASNHISLAF ANDEEISAIG FGSVEEAVSY YSDAAVNGFD QCRFVSLGLQ     50
    DAVVGVEDDD VVMLITGVTQ LRAYLGTFGD RPLNLRGWLL FSNCNYFLEE 100
    LDLVFGRCGG TTIPVDQFMC GADGAPVIQE GDWTFMDYFQ DSNQFTLNGI 150
    TYVKAWDVDR KPNDYAKQNV TCIRRITYIT DHRHVLADGT TMKTARHPKV 200
    NKSVVLDSPF DQIYKEVGSP FMGNGSTFVE MLKDPAFFHA LITCECGRSE 250
    WTVGDWKGYN SLCCNIKCKP ITIVTPKAVP GAVVITKAGI GAGLKCYNNV 300
    FLKHIIDLVV PGTNLGWGVW RIAKVQSKDD VATSGNVLVD DPEDRLDPCY 350
    FGNDGPFATK FKFQLLANSF DDEVKGAIVQ GVVHVNTAIC DVVKDILGLP 400
    WFVKKLGSLV TVMWDQFVAG VQSMKICTLK VVQLAKALSC ATMSVVKGVI 450
    TLVAEVPEIF KRLFYTLTSA LKSLCTSSCD ALVVAGKSFA KIGDYVLLPS 500
    ALVRLVSSKV KGKAQSGIKQ LQFATVVLGD THKVESDRVE FSSVNLKMVD 550
    EEFPLNPVGH TVAVGNQAFF CSDGLYRFMA DRDLVITSPI FKPELELEPI 600
    FECDAIPGFP KVAASNVAEL CVKVDTLLFN YDKIYKKYST IIKGDRCYIQ 650
    CTHTFKAPSY YFDDDEFVEL CTKYYKLPDF DAFYNAVHAA TDMDQFCALC 700
    TSGFEVFIPR VPDCPPILND IDGGSIWTSF ILSVRSATDF IKTLKIDLGL 750
    NGVVVFVTKK FRKAGALLQK LYNAFLDTVT SFIKVAGVAF KYCATCVPKI 800
    VINGCYHTVT RLFAKDLQIP TEDGVADFNT FNHCVFPVNP TRIETDSLEL 850
    EEVDFVEPGV DGKLVILDDY SFYSDGTNYY PSDGKGVVAS CFKKKGGGVV 900
    TISDEVQVRT IDPVYKVRLE YEFEDETLVK VCEKAIGTKL KVTGDWSNLL 950
    ETLEKAMDVV RQHLDVPDYF VYDEEGGTDL NLTIMVSQWP LSSDSEDDFK 1000
    AVDDEPNANT DETVDTFAED VAETQNVQQD VTQDEVEAVC DLVVKATEEG 1050
    PIEHEELSED QKEVQQALAF IEDKPVVVKP DVFAFSYASY GGLKVLNQSS 1100
    NNCWVSSALV QLQLTGLLDS DEMQLFNAGR VSPMVKRCYE SQRAIFGSLG 1150
    DVSACLESLL KDRDGMSITC TIDCGCGPGV RVYENAIFRF TPLKTAFPMG 1200
    RCLICSKTLM HTITQMKGTG IFCRDATALD VDTLVVKPLC AAVYVGAQDG 1250
    GHYLTNMYDA NMAVDGHGRH PIKFNTINTL CYKDVDWEVS NGSCDVKPFL 1300
    TYKNIEFYQG ELSALLSVNH DFVVNAANEQ LSHGGGIAKA LDDLTKGELQ 1350
    VLSNQYVSRN GSIKVGSGVL IKCKEHSILN VVGPRKGKHA AELLTKAYTF 1400
    VFKQKGVPLM PLLSVGIFKV PITESLAAFL ACVGDRVCKC FCYTDKERLA 1450
    IQNFVTSFQT EQPVEPLPVI QEVKGVQLEK PVPDVKVENP CEPFRIEGDA 1500
    KFYDLTPSMV QSLQVTRLVS FTNSDLCLGS FVRDCDGYVQ GSLGGAIANY 1550
    KKSNPVLPAG NCVTLKCDGF ISFTFVILPK EGDTNYEKNF NRAIAKFLKL 1600
    KGSLLVVVED SSVFNKISHA SVAGYVAKPA LVDTLFEAKP VQVVVTQDQR 1650
    SFHTVELSTS QTYGQQLGDC VVEDKKVTNL KPVSKDKVVS VVPNVDWDKH 1700
    YGFVDAGIFH TLDHTMFVFD NNVVNGKRVL RTSDNNCWIN AVCLQLQFAN 1750
    AKFKPKGLQQ LWESYCTGDV AMFVHWLYWI TGVEKGEPSD AENTLNIISR 1800
    FLKPQGSVEM LRATSTTCDG TCSTKRVVST PVVNASVLKV GLDDGNCVHG 1850
    LPLVDRVVSV NGTVIITNVG DTPGKPVVAT ENLLLDGVSY TVFQDSTTGV 1900
    GHYTVFDKEA KLMFDGDVLK PCDLNVSPVT SVVVCNNKKI VVQDPVKRVE 1950
    LDASKFLDTM NVASEKFFTF GDFVSRNIIV LIVYLFSLLA ICFRALKKRD 2000
    MKVMAGVPER TGIILKRSVK YNYKALKFFF RLKFQYIKVF LKFSLVLYTL 2050
    YALMFMFIRF TPVGTPICKR YTDGYANSTF DKNDYCGNVL CKICLYGYEE 2100
    LSDFTHTRVI WQHLKDPLIG NILPLFYLVF LIIFGGFFVR IGITYFIMQY 2150
    INAAGVALGY QDNVWLLHLL PFNSMGNIIV VAFIVTRILL FLKHVLFGCD 2200
    KPSCIACSKS AKLTRVPLQT ILQGVTKSFY VNANGGKKFC KKHNFFCVDC 2250
    DSYGYGCTFI NDVIAPELSN VTKLNVIPTG PATIIIDKVE FSNGFYYLYS 2300
    GSTFWKYNFD ITEAKYACKD VLKNCNILTD FVVFNNSGSN VTQVKNACVY 2350
    FSQLLCKPIK LVDSALLASL NVDFSANLHK AFVEVLSNSF GKDLSNCSNM 2400
    NECRESLGLS DVPEEEFSAA VSEAHRYDVL ISDVSFNNLI VSYAKPEEKL 2450
    AVHDIANCMR VGAKVVNHNV LTKDNVPVVW LAKDFIALSE EARKYIVRTT 2500
    KTKGINFMLT FNDRRMHLTI PTISVANKKG AGLPSLFTRL YSFFWHLCVL 2550
    IVVLFVATSL LDFSAQVTSD TQYDFKYIEN GVLKVFEKPL DCVHNAFVNF 2600
    NEWHNAKFGS IPTNSRRCPI VVGTSDEVRY IPGVPAGVFL YGKSLIFAMS 2650
    TIFGTSGLCF DDRGLTDPDS CIFNSACTTL SGIGGRNVYC YREGVVDNAK 2700
    LYSSLLPHSY YRLMDGNHIV LPEIITRGFG IRTIKTQAMT YCRTGECIDS 2750
    QAGVCVGLDR FFVYSKTPGS DYVCGTGFFS LLFNVIGMFS NSIPVTVMSG 2800
    QILLNCVVAF TAVMACFAFT KFKRLFGDMS FGVLSVGLCT VVNNLSYVVT 2850
    QNSIGMLAYA TLYFLCTKGV RYSWVWHVGF AISYCFLAPW WVVLAYLICA 2900
    LLEFLPNLFK LKVSTQLFEG DKFVGSFESA ASGTFVLDMH SYQKLANSIS 2950
    TEKLKQYCAS YNRYKYYSGS ASEADYRLAC FAHLAKAMSD FANDHMDKLY 3000
    TPPTVSYNST LQAGLRKMAQ PSGIVEGCIV RVSYGNLTLN GLWLGDTVIC 3050
    PRHVIASNTT NVIDYDHAMS LVRLHNFSIS SGNMFLGVIS ASMRGTLLHI 3100
    KVNQSNVNTP NYTYKVLKPG DSFNILACYD GSAAGVYGVN MRTNYTIRGS 3150
    FISGACGSPG YNINNGVVEF CYMHHLELGS GCHVGSDMDG TMYGKYEDQP 3200
    TLQIEGASNL VTENVCSWLY GALINGDRWW LSSVSVGVDT YNEWALRNGM 3250
    TALKNVDCFS LLVAKTGVDV GRLLASIQKL HGNFGGKSIL GCTSLCDEFT 3300
    LSEVVKQMYG VTLQSGKVSR AFRNASIVCC LLFLFLSEML NHSKLFWINP 3350
    GYITPVFLAI IVASSALMLL VKHKLLFLQL YLLPSLCIVS GYNIFKDYHF 3400
    YTYMLEEFDY KVPFGGFNVT GVLNISLCCF VMGLHTFRFL QTPNKIFSYV 3450
    VAVLTVLYTY YYSTDVLGLI LTSMSGFTNY WFIGTATYKL ATYVLPHTSL 3500
    LDSFDAIKAV VFLYLLLGYC NCVYYGSLYW INRFCKLTLG CYEFKVSAAE 3550
    FKYMVANGLR APTGVFDALI LSLKLIGVGG RKTIKISSVQ SKLTDLKCTN 3600
    VVLLGCLSNM NIAANSREWA YCVDLHNKIN LCNDAEAAQE MLLALLAFFL 3650
    SKNSAFGVDE LLDSYFNDSS VLQSVAATYV NLPSYLAYET ARQSYEDALA 3700
    NGSPPQLVKQ LRHAMNVAKS EFDREASTQR KLDRMAEQAA SQMYKEARAV 3750
    NRKSKVVSAM HSLLFGMLRR LDMSSVDTIL SLAKDGVVPL SIIPAVSATK 3800
    LNIVVSDIES YSKIQREGCV HYAGVIWSVV DIKDNDGKPV HAKEVVTSNV 3850
    ESLAWPLFLN CERIIKLQNN EIIPSKIKQR PIKAEGEGVV ADGNALYSNE 3900
    GGRTFMYAFI SDKPDLKVVK WEFDGGSNAI ELEPPCKFLV EAPSGPVVKY 3950
    LYFVRNLNNL RRGAVLGFIG ATVRLQAGKQ TEQATNSSLL TLCAFAVDPP 4000
    KTYLDAVKSG HRPVGNCVKM LANGSGNGQA ITNGVEASTN QDSYGGASVC 4050
    LYCRAHVEHP DMDGFCKLRG KYVQVPLGTL DPIRFVLENT VCKVCGCWQA 4100
    NGCTCDRAVI QSVDSGYLNR VRGSSAARLE PLNGSDTHHV FRAFDVYNRD 4150
    VACISKFLKV NCVRLKNLDK HDAFWIVKKC TKSVMEHEQS IYNLISDCGA 4200
    VAKHDFFTWK EGRSVYGNVC RQDLTEYTMM DLCYALRNFD ENNCETLKKI 4250
    LVVVGACDES YFDNKLWFDP VENEDVHRVY AKLGTIVARA MLKCVKYCDA 4300
    MVEQGIVGVI TLDNQDLNGD FYDFGDFVTS VKGMGVPICT SYYSYMMPVM 4350
    GMTNCLASEC FIKSDIFGED FRTFDLLAYD FTEHKVNLFN KYFKHWGQTY 4400
    HPNCEDCHDE SCIVHCANFN TLFATTIPIT AFGPLCRKCW IDGVPLVTTA 4450
    GYHFKQLGIV WNKDLNLHSS RLTINELLQF CADPSLLIAS SPALVDKRTV 4500
    CFSVAALGTG MTNQTVKPGH FNREFYDFLR SQGFFEEGSE LTLKHFFFAQ 4550
    KGDAAVRDFD YYRYNRTTVL DICQARVVYQ IVQCYFGMYE GGCITAKEVI 4600
    VNNLNKSAGY PFNKFGKAGL YYDSLSYEEQ DDLYAYTKRN IIPTMTQLNL 4650
    KYAISGKDRA RTVGGVSLLS TMTTRQYHQK HLKSIVNTRG ASVVIGTTKF 4700
    YGGWDNMLKT LIKDVENPHL MGWDYPKCDR ALPNMIRMIS AMILGSKHVN 4750
    CCSSSDRYYR LCNELAQVLT EMVYSNGGFY VKPGGTTSGD ATTAYANSVF 4800
    NIFQATSANV NRLLSVDSNT CNNIEVKQLQ RKLYDCCYRS SSVDQSFVEE 4850
    YFGYLRKHFS MMILSDDGVV CYNSEYAALG YVADLNAFKA VLYYQNNVFM 4900
    SASKCWIEPD INKGPHEFCS QHTMQIVDKD GTYYLPYPDP SRILSAGVFV 4950
    DDIVKTDPVI LLERYVSLAI DAYPLSKHDN PEYRRVFTVM LDWVKHLYKT 5000
    LNQGVLDSFS VTLLEDATAK FWDESFYASM YEQSSVLQSA GLCVVCSSQT 5050
    VLRCGDCIRR PMLCTKCAYD HVVSTSHKFI LAITPYVCCS SGCGVSDVTK 5100
    LYLGGLSYWC VDHKPRLSFP LCSSGNVFGL YKNSATGSPD VDDFNTLATS 5150
    DWTDVKDYKL ANDVKDSLRL FAAETIKAKE ESVKSSYACA TIHEVVGPKE 5200
    LVLKWEVGKP RPPLSRNSVF TCYHITKNTK FQVGEFTFEK LDYDNDAVSY 5250
    KSTATTKLVP GMVFVLTSHN VQPLRAPTII NQERYSTLHK LRPAFNIHED 5300
    YSNLIPYYQL IGKQKLTTIQ GPPGSGKSHC VIGLGLYFPG ARIVFTACSH 5350
    AAVDSLCVKA ATAYSSDRCS RIIPQKARIE CYDGFKSNNT SAQYLFSTVN 5400
    ALPEVNADIC VVDEVSMCTN YDLSVINQRV NYRHIVYVGD PQQLPAPRVM 5450
    ITRGVLVPED YNVVTRRMCV LKPDIFLHKC YRCPAEIVNT VSEMVYENQF 5500
    VPVKSESKEC FKIYCRGNVQ VDNGSSINRR QLEVVRMFLA KNPKWAKAVF 5550
    ISPYNSQNYV AGRVLGLQIQ TVDSSQGSEY DYVIYTQTSD TAHASNVNRF 5600
    NVAITRAKKG ILCIMCDREL FDILKFYELK LSDLQVGDGC GLFKDCYKGE 5650
    DNLPPSHAPT FMSLSDNFKT DKDLAVQIGV NGPVKYEHVI SFMGFRFDIN 5700
    VPNQHTLFCT RDFAMRNARG WLGFDVEGAH VIGSNVGTNV PLQLGFSNGV 5750
    DFVVRPEGCV STEVGDVIQP VRARAPPGDQ FTHLLPLLRK GQPWSVIRRR 5800
    IVQMCSDYLA NLSDTLIFVL WSGGLELTTM RYFVKLGPVQ TCDCGKRATC 5850
    YNSTNHTFSC FRHALGSDYI YNCYCIDIQQ WGYTGSLSMN HHEVCNIHRN 5900
    EHVASGDAAM TRCLAIHDCF VKNVDWSITY PFIANEQAIN KSGRLVQSHV 5950
    MRAVLKLYNP KAIHDVGNPK GIRCVVTDAS WYCYDKNPTN TNVKMLEYDY 6000
    ITHGQLDGLC LFWNCNVDMY PEFSVVCRFD TRMRSTLNLE GCNGGSLYVN 6050
    NHAFHTPAYD KRAFAKLKAM PFFFYDDSEC EKLQDAVNYV PLRASNCITR 6100
    CNVGGAVCSK HCALYHNYVM AYNTFTTAGF TIWVPNSFDM FNLWQTFKNS 6150
    NVQGLENIAY NVVKKGSFVG VEGELPVAVV NDKVMVRDGV SDNVVFVNNT 6200
    SLPTNVAFEL YAKRKVGLTP PLTILKNLGV VCTSKCVLWD YEASRPLTTF 6250
    TKDVCKYTDF DGDVCTLFDN SVPGAFERFT VTKNAVLISL TAVKKLTAIK 6300
    LTYGYLNGVP VFTHEDKPFT WYIYTRKDGA FVEYPDGYFT QGRVISDFQP 6350
    RSNMEEDFLN MDMGLFISKY GLEDYGFEHV VFGDVSKTTL GGLHLLISQI 6400
    RLSKIGVLKV EDFVSSSDST LKSCTVTYVD NPSSKMVCTY VDLLLDDFVN 6450
    ILKSVDLSVV SKVHEVVIDC KVWRWMLWCK DHKVQTFYPQ LQSAEWKCGY 6500
    SMPSIYKIQR MCLEPCNLYN YGSGLKLPDG IMFNVVKYTQ LCQYLNSTTM 6550
    CVPHHMRVLH LGAGSDKGVA PGTAVLRRWL PLDAVIVDND VNDYVSDADF 6600
    SYTGDCASMY LTDKFDLVIS DMYDGRTKSC DGDNVSKEGF FPYINGVITE 6650
    KLALGGTVAI KITEFSWNKK LYELIQKFEY WTLFCTSVNT SSSEAFLIGV 6700
    HFLGDFSTNA IIDGNIMHAN YIFWRNSTIM TMSYNSVLDL SKFSCKHKAT 6750
    VVVNLKDSSV TDLVLGLLKN GKLLIRNNGV VCGFSNHLVN STK 6793

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:6,793
    Mass (Da):757,262
    Last modified:June 10, 2008 - v1
    Checksum:i6C76171F3E67FE1D
    GO
    Isoform Replicase polyprotein 1a (identifier: P0C6F6-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    The sequence of this isoform can be found in the external entry P0C6F6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by conventional translation.

    Length:4,128
    Mass (Da):456,581
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ648858 Genomic RNA. Translation: ABG47077.1.
    RefSeqiYP_001351683.1. NC_009657.1. [P0C6W0-1]

    Genome annotation databases

    GeneIDi11266518.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ648858 Genomic RNA. Translation: ABG47077.1 .
    RefSeqi YP_001351683.1. NC_009657.1. [P0C6W0-1 ]

    3D structure databases

    ProteinModelPortali P0C6W0.
    SMRi P0C6W0. Positions 3013-3311, 3871-3975, 3982-4107.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0C6W0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 11266518.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
    IPR009461. Coronavirus_NSP16.
    IPR027352. CV_MBD_dom.
    IPR002589. Macro_dom.
    IPR009466. NSP11.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR027417. P-loop_NTPase.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009469. RNA_pol_N_coronovir.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF06478. Corona_RPol_N. 1 hit.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF06471. NSP11. 1 hit.
    PF06460. NSP13. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF01443. Viral_helicase1. 1 hit.
    PF08715. Viral_protease. 2 hits.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEi PS51653. CV_MBD. 1 hit.
    PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 2 hits.
    PS51657. PSRV_HELICASE. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiR1AB_BC512
    AccessioniPrimary (citable) accession number: P0C6W0
    Secondary accession number(s): Q0Q467
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Bat coronavirus 512/2005 is highly similar to porcine epidemic diarrhea virus (PEDV).

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3