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P0C6W0

- R1AB_BC512

UniProt

P0C6W0 - R1AB_BC512

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Protein

Replicase polyprotein 1ab

Gene
rep, 1a-1b
Organism
Bat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.
Nsp9 is a ssRNA-binding protein By similarity.
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei110 – 1112Cleavage; by PL1-PRO By similarity
Sitei897 – 8982Cleavage; by PL1-PRO By similarity
Active sitei1103 – 11031For PL1-PRO activity By similarity
Active sitei1252 – 12521For PL1-PRO activity By similarity
Active sitei1737 – 17371For PL2-PRO activity By similarity
Active sitei1902 – 19021For PL2-PRO activity By similarity
Sitei2530 – 25312Cleavage; by PL2-PRO By similarity
Sitei3012 – 30132Cleavage; by 3CL-PRO By similarity
Active sitei3053 – 30531For 3CL-PRO activity By similarity
Active sitei3156 – 31561For 3CL-PRO activity By similarity
Sitei3314 – 33152Cleavage; by 3CL-PRO By similarity
Sitei3590 – 35912Cleavage; by 3CL-PRO By similarity
Sitei3673 – 36742Cleavage; by 3CL-PRO By similarity
Sitei3868 – 38692Cleavage; by 3CL-PRO By similarity
Sitei3976 – 39772Cleavage; by 3CL-PRO By similarity
Sitei4111 – 41122Cleavage; by 3CL-PRO By similarity
Sitei5038 – 50392Cleavage; by 3CL-PRO By similarity
Sitei5557 – 55582Cleavage; by 3CL-PRO By similarity
Sitei6153 – 61542Cleavage; by 3CL-PRO By similarity
Sitei6492 – 64932Cleavage; by 3CL-PRO By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1174 – 120532C4-type By similarityAdd
BLAST
Zinc fingeri4050 – 406617 By similarityAdd
BLAST
Zinc fingeri4092 – 410514 By similarityAdd
BLAST
Nucleotide bindingi5321 – 53288ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. methyltransferase activity Source: InterPro
  7. omega peptidase activity Source: InterPro
  8. RNA binding Source: UniProtKB-KW
  9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of host autophagy Source: UniProtKB-KW
  2. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  3. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
  5. viral protein processing Source: InterPro
  6. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p9
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p87
Alternative name(s):
PL1-PRO/PL2-PRO
PLP1/PLP2
Papain-like proteinases 1/2
p195
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p34
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p5
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p23
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p14
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
p100
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
p66
p66-HEL
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
Taxonomic identifieri693999 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostiScotophilus kuhlii (Lesser asiatic yellow bat) [TaxID: 153297]

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1973 – 199321Helical; Reviewed predictionAdd
BLAST
Transmembranei2036 – 205621Helical; Reviewed predictionAdd
BLAST
Transmembranei2119 – 213921Helical; Reviewed predictionAdd
BLAST
Transmembranei2141 – 216121Helical; Reviewed predictionAdd
BLAST
Transmembranei2164 – 218421Helical; Reviewed predictionAdd
BLAST
Transmembranei2543 – 256321Helical; Reviewed predictionAdd
BLAST
Transmembranei2634 – 265421Helical; Reviewed predictionAdd
BLAST
Transmembranei2669 – 268921Helical; Reviewed predictionAdd
BLAST
Transmembranei2769 – 278921Helical; Reviewed predictionAdd
BLAST
Transmembranei2802 – 282221Helical; Reviewed predictionAdd
BLAST
Transmembranei2829 – 284921Helical; Reviewed predictionAdd
BLAST
Transmembranei2878 – 289821Helical; Reviewed predictionAdd
BLAST
Transmembranei3351 – 337121Helical; Reviewed predictionAdd
BLAST
Transmembranei3376 – 339621Helical; Reviewed predictionAdd
BLAST
Transmembranei3414 – 343421Helical; Reviewed predictionAdd
BLAST
Transmembranei3443 – 346321Helical; Reviewed predictionAdd
BLAST
Transmembranei3466 – 348621Helical; Reviewed predictionAdd
BLAST
Transmembranei3488 – 350720Helical; Reviewed predictionAdd
BLAST
Transmembranei3511 – 353121Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 110110Non-structural protein 1 By similarityPRO_0000289911Add
BLAST
Chaini111 – 897787Non-structural protein 2 By similarityPRO_0000289912Add
BLAST
Chaini898 – 25301633Non-structural protein 3 By similarityPRO_0000289913Add
BLAST
Chaini2531 – 3012482Non-structural protein 4 By similarityPRO_0000289914Add
BLAST
Chaini3013 – 33143023C-like proteinase By similarityPRO_0000289915Add
BLAST
Chaini3315 – 3590276Non-structural protein 6 By similarityPRO_0000289916Add
BLAST
Chaini3591 – 367383Non-structural protein 7 By similarityPRO_0000289917Add
BLAST
Chaini3674 – 3868195Non-structural protein 8 By similarityPRO_0000289918Add
BLAST
Chaini3869 – 3976108Non-structural protein 9 By similarityPRO_0000289919Add
BLAST
Chaini3977 – 4111135Non-structural protein 10 By similarityPRO_0000289920Add
BLAST
Chaini4112 – 5038927RNA-directed RNA polymerase By similarityPRO_0000289921Add
BLAST
Chaini5039 – 5557519Helicase By similarityPRO_0000289922Add
BLAST
Chaini5558 – 6153596Exoribonuclease By similarityPRO_0000289923Add
BLAST
Chaini6154 – 6492339Uridylate-specific endoribonuclease By similarityPRO_0000289924Add
BLAST
Chaini6493 – 6793301Putative 2'-O-methyl transferase By similarityPRO_0000289925Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Proteomic databases

PRIDEiP0C6W0.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6W0.
SMRiP0C6W0. Positions 3013-3311, 3871-3975, 3982-4107.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1069 – 1302234Peptidase C16 1Add
BLAST
Domaini1303 – 1467165MacroAdd
BLAST
Domaini1699 – 1965267Peptidase C16 2Add
BLAST
Domaini3013 – 3314302Peptidase C30Add
BLAST
Domaini5039 – 512284CV MBDAdd
BLAST
Domaini5296 – 5477182(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini5478 – 5647170(+)RNA virus helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1973 – 2184212HD1 By similarityAdd
BLAST
Regioni2543 – 2898356HD2 By similarityAdd
BLAST
Regioni3351 – 3531181HD3 By similarityAdd
BLAST
Regioni4649 – 4951303RNA-directed RNA polymerase By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1827 – 1946120Val-richAdd
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6W0-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MASNHISLAF ANDEEISAIG FGSVEEAVSY YSDAAVNGFD QCRFVSLGLQ     50
DAVVGVEDDD VVMLITGVTQ LRAYLGTFGD RPLNLRGWLL FSNCNYFLEE 100
LDLVFGRCGG TTIPVDQFMC GADGAPVIQE GDWTFMDYFQ DSNQFTLNGI 150
TYVKAWDVDR KPNDYAKQNV TCIRRITYIT DHRHVLADGT TMKTARHPKV 200
NKSVVLDSPF DQIYKEVGSP FMGNGSTFVE MLKDPAFFHA LITCECGRSE 250
WTVGDWKGYN SLCCNIKCKP ITIVTPKAVP GAVVITKAGI GAGLKCYNNV 300
FLKHIIDLVV PGTNLGWGVW RIAKVQSKDD VATSGNVLVD DPEDRLDPCY 350
FGNDGPFATK FKFQLLANSF DDEVKGAIVQ GVVHVNTAIC DVVKDILGLP 400
WFVKKLGSLV TVMWDQFVAG VQSMKICTLK VVQLAKALSC ATMSVVKGVI 450
TLVAEVPEIF KRLFYTLTSA LKSLCTSSCD ALVVAGKSFA KIGDYVLLPS 500
ALVRLVSSKV KGKAQSGIKQ LQFATVVLGD THKVESDRVE FSSVNLKMVD 550
EEFPLNPVGH TVAVGNQAFF CSDGLYRFMA DRDLVITSPI FKPELELEPI 600
FECDAIPGFP KVAASNVAEL CVKVDTLLFN YDKIYKKYST IIKGDRCYIQ 650
CTHTFKAPSY YFDDDEFVEL CTKYYKLPDF DAFYNAVHAA TDMDQFCALC 700
TSGFEVFIPR VPDCPPILND IDGGSIWTSF ILSVRSATDF IKTLKIDLGL 750
NGVVVFVTKK FRKAGALLQK LYNAFLDTVT SFIKVAGVAF KYCATCVPKI 800
VINGCYHTVT RLFAKDLQIP TEDGVADFNT FNHCVFPVNP TRIETDSLEL 850
EEVDFVEPGV DGKLVILDDY SFYSDGTNYY PSDGKGVVAS CFKKKGGGVV 900
TISDEVQVRT IDPVYKVRLE YEFEDETLVK VCEKAIGTKL KVTGDWSNLL 950
ETLEKAMDVV RQHLDVPDYF VYDEEGGTDL NLTIMVSQWP LSSDSEDDFK 1000
AVDDEPNANT DETVDTFAED VAETQNVQQD VTQDEVEAVC DLVVKATEEG 1050
PIEHEELSED QKEVQQALAF IEDKPVVVKP DVFAFSYASY GGLKVLNQSS 1100
NNCWVSSALV QLQLTGLLDS DEMQLFNAGR VSPMVKRCYE SQRAIFGSLG 1150
DVSACLESLL KDRDGMSITC TIDCGCGPGV RVYENAIFRF TPLKTAFPMG 1200
RCLICSKTLM HTITQMKGTG IFCRDATALD VDTLVVKPLC AAVYVGAQDG 1250
GHYLTNMYDA NMAVDGHGRH PIKFNTINTL CYKDVDWEVS NGSCDVKPFL 1300
TYKNIEFYQG ELSALLSVNH DFVVNAANEQ LSHGGGIAKA LDDLTKGELQ 1350
VLSNQYVSRN GSIKVGSGVL IKCKEHSILN VVGPRKGKHA AELLTKAYTF 1400
VFKQKGVPLM PLLSVGIFKV PITESLAAFL ACVGDRVCKC FCYTDKERLA 1450
IQNFVTSFQT EQPVEPLPVI QEVKGVQLEK PVPDVKVENP CEPFRIEGDA 1500
KFYDLTPSMV QSLQVTRLVS FTNSDLCLGS FVRDCDGYVQ GSLGGAIANY 1550
KKSNPVLPAG NCVTLKCDGF ISFTFVILPK EGDTNYEKNF NRAIAKFLKL 1600
KGSLLVVVED SSVFNKISHA SVAGYVAKPA LVDTLFEAKP VQVVVTQDQR 1650
SFHTVELSTS QTYGQQLGDC VVEDKKVTNL KPVSKDKVVS VVPNVDWDKH 1700
YGFVDAGIFH TLDHTMFVFD NNVVNGKRVL RTSDNNCWIN AVCLQLQFAN 1750
AKFKPKGLQQ LWESYCTGDV AMFVHWLYWI TGVEKGEPSD AENTLNIISR 1800
FLKPQGSVEM LRATSTTCDG TCSTKRVVST PVVNASVLKV GLDDGNCVHG 1850
LPLVDRVVSV NGTVIITNVG DTPGKPVVAT ENLLLDGVSY TVFQDSTTGV 1900
GHYTVFDKEA KLMFDGDVLK PCDLNVSPVT SVVVCNNKKI VVQDPVKRVE 1950
LDASKFLDTM NVASEKFFTF GDFVSRNIIV LIVYLFSLLA ICFRALKKRD 2000
MKVMAGVPER TGIILKRSVK YNYKALKFFF RLKFQYIKVF LKFSLVLYTL 2050
YALMFMFIRF TPVGTPICKR YTDGYANSTF DKNDYCGNVL CKICLYGYEE 2100
LSDFTHTRVI WQHLKDPLIG NILPLFYLVF LIIFGGFFVR IGITYFIMQY 2150
INAAGVALGY QDNVWLLHLL PFNSMGNIIV VAFIVTRILL FLKHVLFGCD 2200
KPSCIACSKS AKLTRVPLQT ILQGVTKSFY VNANGGKKFC KKHNFFCVDC 2250
DSYGYGCTFI NDVIAPELSN VTKLNVIPTG PATIIIDKVE FSNGFYYLYS 2300
GSTFWKYNFD ITEAKYACKD VLKNCNILTD FVVFNNSGSN VTQVKNACVY 2350
FSQLLCKPIK LVDSALLASL NVDFSANLHK AFVEVLSNSF GKDLSNCSNM 2400
NECRESLGLS DVPEEEFSAA VSEAHRYDVL ISDVSFNNLI VSYAKPEEKL 2450
AVHDIANCMR VGAKVVNHNV LTKDNVPVVW LAKDFIALSE EARKYIVRTT 2500
KTKGINFMLT FNDRRMHLTI PTISVANKKG AGLPSLFTRL YSFFWHLCVL 2550
IVVLFVATSL LDFSAQVTSD TQYDFKYIEN GVLKVFEKPL DCVHNAFVNF 2600
NEWHNAKFGS IPTNSRRCPI VVGTSDEVRY IPGVPAGVFL YGKSLIFAMS 2650
TIFGTSGLCF DDRGLTDPDS CIFNSACTTL SGIGGRNVYC YREGVVDNAK 2700
LYSSLLPHSY YRLMDGNHIV LPEIITRGFG IRTIKTQAMT YCRTGECIDS 2750
QAGVCVGLDR FFVYSKTPGS DYVCGTGFFS LLFNVIGMFS NSIPVTVMSG 2800
QILLNCVVAF TAVMACFAFT KFKRLFGDMS FGVLSVGLCT VVNNLSYVVT 2850
QNSIGMLAYA TLYFLCTKGV RYSWVWHVGF AISYCFLAPW WVVLAYLICA 2900
LLEFLPNLFK LKVSTQLFEG DKFVGSFESA ASGTFVLDMH SYQKLANSIS 2950
TEKLKQYCAS YNRYKYYSGS ASEADYRLAC FAHLAKAMSD FANDHMDKLY 3000
TPPTVSYNST LQAGLRKMAQ PSGIVEGCIV RVSYGNLTLN GLWLGDTVIC 3050
PRHVIASNTT NVIDYDHAMS LVRLHNFSIS SGNMFLGVIS ASMRGTLLHI 3100
KVNQSNVNTP NYTYKVLKPG DSFNILACYD GSAAGVYGVN MRTNYTIRGS 3150
FISGACGSPG YNINNGVVEF CYMHHLELGS GCHVGSDMDG TMYGKYEDQP 3200
TLQIEGASNL VTENVCSWLY GALINGDRWW LSSVSVGVDT YNEWALRNGM 3250
TALKNVDCFS LLVAKTGVDV GRLLASIQKL HGNFGGKSIL GCTSLCDEFT 3300
LSEVVKQMYG VTLQSGKVSR AFRNASIVCC LLFLFLSEML NHSKLFWINP 3350
GYITPVFLAI IVASSALMLL VKHKLLFLQL YLLPSLCIVS GYNIFKDYHF 3400
YTYMLEEFDY KVPFGGFNVT GVLNISLCCF VMGLHTFRFL QTPNKIFSYV 3450
VAVLTVLYTY YYSTDVLGLI LTSMSGFTNY WFIGTATYKL ATYVLPHTSL 3500
LDSFDAIKAV VFLYLLLGYC NCVYYGSLYW INRFCKLTLG CYEFKVSAAE 3550
FKYMVANGLR APTGVFDALI LSLKLIGVGG RKTIKISSVQ SKLTDLKCTN 3600
VVLLGCLSNM NIAANSREWA YCVDLHNKIN LCNDAEAAQE MLLALLAFFL 3650
SKNSAFGVDE LLDSYFNDSS VLQSVAATYV NLPSYLAYET ARQSYEDALA 3700
NGSPPQLVKQ LRHAMNVAKS EFDREASTQR KLDRMAEQAA SQMYKEARAV 3750
NRKSKVVSAM HSLLFGMLRR LDMSSVDTIL SLAKDGVVPL SIIPAVSATK 3800
LNIVVSDIES YSKIQREGCV HYAGVIWSVV DIKDNDGKPV HAKEVVTSNV 3850
ESLAWPLFLN CERIIKLQNN EIIPSKIKQR PIKAEGEGVV ADGNALYSNE 3900
GGRTFMYAFI SDKPDLKVVK WEFDGGSNAI ELEPPCKFLV EAPSGPVVKY 3950
LYFVRNLNNL RRGAVLGFIG ATVRLQAGKQ TEQATNSSLL TLCAFAVDPP 4000
KTYLDAVKSG HRPVGNCVKM LANGSGNGQA ITNGVEASTN QDSYGGASVC 4050
LYCRAHVEHP DMDGFCKLRG KYVQVPLGTL DPIRFVLENT VCKVCGCWQA 4100
NGCTCDRAVI QSVDSGYLNR VRGSSAARLE PLNGSDTHHV FRAFDVYNRD 4150
VACISKFLKV NCVRLKNLDK HDAFWIVKKC TKSVMEHEQS IYNLISDCGA 4200
VAKHDFFTWK EGRSVYGNVC RQDLTEYTMM DLCYALRNFD ENNCETLKKI 4250
LVVVGACDES YFDNKLWFDP VENEDVHRVY AKLGTIVARA MLKCVKYCDA 4300
MVEQGIVGVI TLDNQDLNGD FYDFGDFVTS VKGMGVPICT SYYSYMMPVM 4350
GMTNCLASEC FIKSDIFGED FRTFDLLAYD FTEHKVNLFN KYFKHWGQTY 4400
HPNCEDCHDE SCIVHCANFN TLFATTIPIT AFGPLCRKCW IDGVPLVTTA 4450
GYHFKQLGIV WNKDLNLHSS RLTINELLQF CADPSLLIAS SPALVDKRTV 4500
CFSVAALGTG MTNQTVKPGH FNREFYDFLR SQGFFEEGSE LTLKHFFFAQ 4550
KGDAAVRDFD YYRYNRTTVL DICQARVVYQ IVQCYFGMYE GGCITAKEVI 4600
VNNLNKSAGY PFNKFGKAGL YYDSLSYEEQ DDLYAYTKRN IIPTMTQLNL 4650
KYAISGKDRA RTVGGVSLLS TMTTRQYHQK HLKSIVNTRG ASVVIGTTKF 4700
YGGWDNMLKT LIKDVENPHL MGWDYPKCDR ALPNMIRMIS AMILGSKHVN 4750
CCSSSDRYYR LCNELAQVLT EMVYSNGGFY VKPGGTTSGD ATTAYANSVF 4800
NIFQATSANV NRLLSVDSNT CNNIEVKQLQ RKLYDCCYRS SSVDQSFVEE 4850
YFGYLRKHFS MMILSDDGVV CYNSEYAALG YVADLNAFKA VLYYQNNVFM 4900
SASKCWIEPD INKGPHEFCS QHTMQIVDKD GTYYLPYPDP SRILSAGVFV 4950
DDIVKTDPVI LLERYVSLAI DAYPLSKHDN PEYRRVFTVM LDWVKHLYKT 5000
LNQGVLDSFS VTLLEDATAK FWDESFYASM YEQSSVLQSA GLCVVCSSQT 5050
VLRCGDCIRR PMLCTKCAYD HVVSTSHKFI LAITPYVCCS SGCGVSDVTK 5100
LYLGGLSYWC VDHKPRLSFP LCSSGNVFGL YKNSATGSPD VDDFNTLATS 5150
DWTDVKDYKL ANDVKDSLRL FAAETIKAKE ESVKSSYACA TIHEVVGPKE 5200
LVLKWEVGKP RPPLSRNSVF TCYHITKNTK FQVGEFTFEK LDYDNDAVSY 5250
KSTATTKLVP GMVFVLTSHN VQPLRAPTII NQERYSTLHK LRPAFNIHED 5300
YSNLIPYYQL IGKQKLTTIQ GPPGSGKSHC VIGLGLYFPG ARIVFTACSH 5350
AAVDSLCVKA ATAYSSDRCS RIIPQKARIE CYDGFKSNNT SAQYLFSTVN 5400
ALPEVNADIC VVDEVSMCTN YDLSVINQRV NYRHIVYVGD PQQLPAPRVM 5450
ITRGVLVPED YNVVTRRMCV LKPDIFLHKC YRCPAEIVNT VSEMVYENQF 5500
VPVKSESKEC FKIYCRGNVQ VDNGSSINRR QLEVVRMFLA KNPKWAKAVF 5550
ISPYNSQNYV AGRVLGLQIQ TVDSSQGSEY DYVIYTQTSD TAHASNVNRF 5600
NVAITRAKKG ILCIMCDREL FDILKFYELK LSDLQVGDGC GLFKDCYKGE 5650
DNLPPSHAPT FMSLSDNFKT DKDLAVQIGV NGPVKYEHVI SFMGFRFDIN 5700
VPNQHTLFCT RDFAMRNARG WLGFDVEGAH VIGSNVGTNV PLQLGFSNGV 5750
DFVVRPEGCV STEVGDVIQP VRARAPPGDQ FTHLLPLLRK GQPWSVIRRR 5800
IVQMCSDYLA NLSDTLIFVL WSGGLELTTM RYFVKLGPVQ TCDCGKRATC 5850
YNSTNHTFSC FRHALGSDYI YNCYCIDIQQ WGYTGSLSMN HHEVCNIHRN 5900
EHVASGDAAM TRCLAIHDCF VKNVDWSITY PFIANEQAIN KSGRLVQSHV 5950
MRAVLKLYNP KAIHDVGNPK GIRCVVTDAS WYCYDKNPTN TNVKMLEYDY 6000
ITHGQLDGLC LFWNCNVDMY PEFSVVCRFD TRMRSTLNLE GCNGGSLYVN 6050
NHAFHTPAYD KRAFAKLKAM PFFFYDDSEC EKLQDAVNYV PLRASNCITR 6100
CNVGGAVCSK HCALYHNYVM AYNTFTTAGF TIWVPNSFDM FNLWQTFKNS 6150
NVQGLENIAY NVVKKGSFVG VEGELPVAVV NDKVMVRDGV SDNVVFVNNT 6200
SLPTNVAFEL YAKRKVGLTP PLTILKNLGV VCTSKCVLWD YEASRPLTTF 6250
TKDVCKYTDF DGDVCTLFDN SVPGAFERFT VTKNAVLISL TAVKKLTAIK 6300
LTYGYLNGVP VFTHEDKPFT WYIYTRKDGA FVEYPDGYFT QGRVISDFQP 6350
RSNMEEDFLN MDMGLFISKY GLEDYGFEHV VFGDVSKTTL GGLHLLISQI 6400
RLSKIGVLKV EDFVSSSDST LKSCTVTYVD NPSSKMVCTY VDLLLDDFVN 6450
ILKSVDLSVV SKVHEVVIDC KVWRWMLWCK DHKVQTFYPQ LQSAEWKCGY 6500
SMPSIYKIQR MCLEPCNLYN YGSGLKLPDG IMFNVVKYTQ LCQYLNSTTM 6550
CVPHHMRVLH LGAGSDKGVA PGTAVLRRWL PLDAVIVDND VNDYVSDADF 6600
SYTGDCASMY LTDKFDLVIS DMYDGRTKSC DGDNVSKEGF FPYINGVITE 6650
KLALGGTVAI KITEFSWNKK LYELIQKFEY WTLFCTSVNT SSSEAFLIGV 6700
HFLGDFSTNA IIDGNIMHAN YIFWRNSTIM TMSYNSVLDL SKFSCKHKAT 6750
VVVNLKDSSV TDLVLGLLKN GKLLIRNNGV VCGFSNHLVN STK 6793

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:6,793
Mass (Da):757,262
Last modified:June 10, 2008 - v1
Checksum:i6C76171F3E67FE1D
GO
Isoform Replicase polyprotein 1a (identifier: P0C6F6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6F6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,128
Mass (Da):456,581
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648858 Genomic RNA. Translation: ABG47077.1.
RefSeqiYP_001351683.1. NC_009657.1. [P0C6W0-1]

Genome annotation databases

GeneIDi11266518.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648858 Genomic RNA. Translation: ABG47077.1 .
RefSeqi YP_001351683.1. NC_009657.1. [P0C6W0-1 ]

3D structure databases

ProteinModelPortali P0C6W0.
SMRi P0C6W0. Positions 3013-3311, 3871-3975, 3982-4107.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0C6W0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 11266518.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1AB_BC512
AccessioniPrimary (citable) accession number: P0C6W0
Secondary accession number(s): Q0Q467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus 512/2005 is highly similar to porcine epidemic diarrhea virus (PEDV).

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi