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P0C6W0 (R1AB_BC512) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p9
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p87
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.-
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PLP1/PLP2
    Papain-like proteinases 1/2
    p195
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p34
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p5
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p23
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p14
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
    p100
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
    p66
    p66-HEL
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismBat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)
Taxonomic identifier693999 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostScotophilus kuhlii (Lesser asiatic yellow bat) [TaxID: 153297]

Protein attributes

Sequence length6793 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Miscellaneous

Bat coronavirus 512/2005 is highly similar to porcine epidemic diarrhea virus (PEDV).

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 (+)RNA virus helicase ATP-binding domain.

Contains 1 (+)RNA virus helicase C-terminal domain.

Contains 1 CV MBD (coronavirus metal-binding) domain.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
Gene Ontology (GO)
   Biological_processinduction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host IRF3 activity

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: P0C6W0-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: P0C6F6-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry P0C6F6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 110110Non-structural protein 1 By similarity
PRO_0000289911
Chain111 – 897787Non-structural protein 2 By similarity
PRO_0000289912
Chain898 – 25301633Non-structural protein 3 By similarity
PRO_0000289913
Chain2531 – 3012482Non-structural protein 4 By similarity
PRO_0000289914
Chain3013 – 33143023C-like proteinase By similarity
PRO_0000289915
Chain3315 – 3590276Non-structural protein 6 By similarity
PRO_0000289916
Chain3591 – 367383Non-structural protein 7 By similarity
PRO_0000289917
Chain3674 – 3868195Non-structural protein 8 By similarity
PRO_0000289918
Chain3869 – 3976108Non-structural protein 9 By similarity
PRO_0000289919
Chain3977 – 4111135Non-structural protein 10 By similarity
PRO_0000289920
Chain4112 – 5038927RNA-directed RNA polymerase By similarity
PRO_0000289921
Chain5039 – 5557519Helicase By similarity
PRO_0000289922
Chain5558 – 6153596Exoribonuclease By similarity
PRO_0000289923
Chain6154 – 6492339Uridylate-specific endoribonuclease By similarity
PRO_0000289924
Chain6493 – 6793301Putative 2'-O-methyl transferase By similarity
PRO_0000289925

Regions

Transmembrane1973 – 199321Helical; Potential
Transmembrane2036 – 205621Helical; Potential
Transmembrane2119 – 213921Helical; Potential
Transmembrane2141 – 216121Helical; Potential
Transmembrane2164 – 218421Helical; Potential
Transmembrane2543 – 256321Helical; Potential
Transmembrane2634 – 265421Helical; Potential
Transmembrane2669 – 268921Helical; Potential
Transmembrane2769 – 278921Helical; Potential
Transmembrane2802 – 282221Helical; Potential
Transmembrane2829 – 284921Helical; Potential
Transmembrane2878 – 289821Helical; Potential
Transmembrane3351 – 337121Helical; Potential
Transmembrane3376 – 339621Helical; Potential
Transmembrane3414 – 343421Helical; Potential
Transmembrane3443 – 346321Helical; Potential
Transmembrane3466 – 348621Helical; Potential
Transmembrane3488 – 350720Helical; Potential
Transmembrane3511 – 353121Helical; Potential
Domain1069 – 1302234Peptidase C16 1
Domain1303 – 1467165Macro
Domain1699 – 1965267Peptidase C16 2
Domain3013 – 3314302Peptidase C30
Domain5039 – 512284CV MBD
Domain5296 – 5477182(+)RNA virus helicase ATP-binding
Domain5478 – 5647170(+)RNA virus helicase C-terminal
Zinc finger1174 – 120532C4-type By similarity
Zinc finger4050 – 406617 By similarity
Zinc finger4092 – 410514 By similarity
Nucleotide binding5321 – 53288ATP By similarity
Region1973 – 2184212HD1 By similarity
Region2543 – 2898356HD2 By similarity
Region3351 – 3531181HD3 By similarity
Region4649 – 4951303RNA-directed RNA polymerase By similarity
Compositional bias1827 – 1946120Val-rich

Sites

Active site11031For PL1-PRO activity By similarity
Active site12521For PL1-PRO activity By similarity
Active site17371For PL2-PRO activity By similarity
Active site19021For PL2-PRO activity By similarity
Active site30531For 3CL-PRO activity By similarity
Active site31561For 3CL-PRO activity By similarity
Site110 – 1112Cleavage; by PL1-PRO By similarity
Site897 – 8982Cleavage; by PL1-PRO By similarity
Site2530 – 25312Cleavage; by PL2-PRO By similarity
Site3012 – 30132Cleavage; by 3CL-PRO By similarity
Site3314 – 33152Cleavage; by 3CL-PRO By similarity
Site3590 – 35912Cleavage; by 3CL-PRO By similarity
Site3673 – 36742Cleavage; by 3CL-PRO By similarity
Site3868 – 38692Cleavage; by 3CL-PRO By similarity
Site3976 – 39772Cleavage; by 3CL-PRO By similarity
Site4111 – 41122Cleavage; by 3CL-PRO By similarity
Site5038 – 50392Cleavage; by 3CL-PRO By similarity
Site5557 – 55582Cleavage; by 3CL-PRO By similarity
Site6153 – 61542Cleavage; by 3CL-PRO By similarity
Site6492 – 64932Cleavage; by 3CL-PRO By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 6C76171F3E67FE1D

FASTA6,793757,262
        10         20         30         40         50         60 
MASNHISLAF ANDEEISAIG FGSVEEAVSY YSDAAVNGFD QCRFVSLGLQ DAVVGVEDDD 

        70         80         90        100        110        120 
VVMLITGVTQ LRAYLGTFGD RPLNLRGWLL FSNCNYFLEE LDLVFGRCGG TTIPVDQFMC 

       130        140        150        160        170        180 
GADGAPVIQE GDWTFMDYFQ DSNQFTLNGI TYVKAWDVDR KPNDYAKQNV TCIRRITYIT 

       190        200        210        220        230        240 
DHRHVLADGT TMKTARHPKV NKSVVLDSPF DQIYKEVGSP FMGNGSTFVE MLKDPAFFHA 

       250        260        270        280        290        300 
LITCECGRSE WTVGDWKGYN SLCCNIKCKP ITIVTPKAVP GAVVITKAGI GAGLKCYNNV 

       310        320        330        340        350        360 
FLKHIIDLVV PGTNLGWGVW RIAKVQSKDD VATSGNVLVD DPEDRLDPCY FGNDGPFATK 

       370        380        390        400        410        420 
FKFQLLANSF DDEVKGAIVQ GVVHVNTAIC DVVKDILGLP WFVKKLGSLV TVMWDQFVAG 

       430        440        450        460        470        480 
VQSMKICTLK VVQLAKALSC ATMSVVKGVI TLVAEVPEIF KRLFYTLTSA LKSLCTSSCD 

       490        500        510        520        530        540 
ALVVAGKSFA KIGDYVLLPS ALVRLVSSKV KGKAQSGIKQ LQFATVVLGD THKVESDRVE 

       550        560        570        580        590        600 
FSSVNLKMVD EEFPLNPVGH TVAVGNQAFF CSDGLYRFMA DRDLVITSPI FKPELELEPI 

       610        620        630        640        650        660 
FECDAIPGFP KVAASNVAEL CVKVDTLLFN YDKIYKKYST IIKGDRCYIQ CTHTFKAPSY 

       670        680        690        700        710        720 
YFDDDEFVEL CTKYYKLPDF DAFYNAVHAA TDMDQFCALC TSGFEVFIPR VPDCPPILND 

       730        740        750        760        770        780 
IDGGSIWTSF ILSVRSATDF IKTLKIDLGL NGVVVFVTKK FRKAGALLQK LYNAFLDTVT 

       790        800        810        820        830        840 
SFIKVAGVAF KYCATCVPKI VINGCYHTVT RLFAKDLQIP TEDGVADFNT FNHCVFPVNP 

       850        860        870        880        890        900 
TRIETDSLEL EEVDFVEPGV DGKLVILDDY SFYSDGTNYY PSDGKGVVAS CFKKKGGGVV 

       910        920        930        940        950        960 
TISDEVQVRT IDPVYKVRLE YEFEDETLVK VCEKAIGTKL KVTGDWSNLL ETLEKAMDVV 

       970        980        990       1000       1010       1020 
RQHLDVPDYF VYDEEGGTDL NLTIMVSQWP LSSDSEDDFK AVDDEPNANT DETVDTFAED 

      1030       1040       1050       1060       1070       1080 
VAETQNVQQD VTQDEVEAVC DLVVKATEEG PIEHEELSED QKEVQQALAF IEDKPVVVKP 

      1090       1100       1110       1120       1130       1140 
DVFAFSYASY GGLKVLNQSS NNCWVSSALV QLQLTGLLDS DEMQLFNAGR VSPMVKRCYE 

      1150       1160       1170       1180       1190       1200 
SQRAIFGSLG DVSACLESLL KDRDGMSITC TIDCGCGPGV RVYENAIFRF TPLKTAFPMG 

      1210       1220       1230       1240       1250       1260 
RCLICSKTLM HTITQMKGTG IFCRDATALD VDTLVVKPLC AAVYVGAQDG GHYLTNMYDA 

      1270       1280       1290       1300       1310       1320 
NMAVDGHGRH PIKFNTINTL CYKDVDWEVS NGSCDVKPFL TYKNIEFYQG ELSALLSVNH 

      1330       1340       1350       1360       1370       1380 
DFVVNAANEQ LSHGGGIAKA LDDLTKGELQ VLSNQYVSRN GSIKVGSGVL IKCKEHSILN 

      1390       1400       1410       1420       1430       1440 
VVGPRKGKHA AELLTKAYTF VFKQKGVPLM PLLSVGIFKV PITESLAAFL ACVGDRVCKC 

      1450       1460       1470       1480       1490       1500 
FCYTDKERLA IQNFVTSFQT EQPVEPLPVI QEVKGVQLEK PVPDVKVENP CEPFRIEGDA 

      1510       1520       1530       1540       1550       1560 
KFYDLTPSMV QSLQVTRLVS FTNSDLCLGS FVRDCDGYVQ GSLGGAIANY KKSNPVLPAG 

      1570       1580       1590       1600       1610       1620 
NCVTLKCDGF ISFTFVILPK EGDTNYEKNF NRAIAKFLKL KGSLLVVVED SSVFNKISHA 

      1630       1640       1650       1660       1670       1680 
SVAGYVAKPA LVDTLFEAKP VQVVVTQDQR SFHTVELSTS QTYGQQLGDC VVEDKKVTNL 

      1690       1700       1710       1720       1730       1740 
KPVSKDKVVS VVPNVDWDKH YGFVDAGIFH TLDHTMFVFD NNVVNGKRVL RTSDNNCWIN 

      1750       1760       1770       1780       1790       1800 
AVCLQLQFAN AKFKPKGLQQ LWESYCTGDV AMFVHWLYWI TGVEKGEPSD AENTLNIISR 

      1810       1820       1830       1840       1850       1860 
FLKPQGSVEM LRATSTTCDG TCSTKRVVST PVVNASVLKV GLDDGNCVHG LPLVDRVVSV 

      1870       1880       1890       1900       1910       1920 
NGTVIITNVG DTPGKPVVAT ENLLLDGVSY TVFQDSTTGV GHYTVFDKEA KLMFDGDVLK 

      1930       1940       1950       1960       1970       1980 
PCDLNVSPVT SVVVCNNKKI VVQDPVKRVE LDASKFLDTM NVASEKFFTF GDFVSRNIIV 

      1990       2000       2010       2020       2030       2040 
LIVYLFSLLA ICFRALKKRD MKVMAGVPER TGIILKRSVK YNYKALKFFF RLKFQYIKVF 

      2050       2060       2070       2080       2090       2100 
LKFSLVLYTL YALMFMFIRF TPVGTPICKR YTDGYANSTF DKNDYCGNVL CKICLYGYEE 

      2110       2120       2130       2140       2150       2160 
LSDFTHTRVI WQHLKDPLIG NILPLFYLVF LIIFGGFFVR IGITYFIMQY INAAGVALGY 

      2170       2180       2190       2200       2210       2220 
QDNVWLLHLL PFNSMGNIIV VAFIVTRILL FLKHVLFGCD KPSCIACSKS AKLTRVPLQT 

      2230       2240       2250       2260       2270       2280 
ILQGVTKSFY VNANGGKKFC KKHNFFCVDC DSYGYGCTFI NDVIAPELSN VTKLNVIPTG 

      2290       2300       2310       2320       2330       2340 
PATIIIDKVE FSNGFYYLYS GSTFWKYNFD ITEAKYACKD VLKNCNILTD FVVFNNSGSN 

      2350       2360       2370       2380       2390       2400 
VTQVKNACVY FSQLLCKPIK LVDSALLASL NVDFSANLHK AFVEVLSNSF GKDLSNCSNM 

      2410       2420       2430       2440       2450       2460 
NECRESLGLS DVPEEEFSAA VSEAHRYDVL ISDVSFNNLI VSYAKPEEKL AVHDIANCMR 

      2470       2480       2490       2500       2510       2520 
VGAKVVNHNV LTKDNVPVVW LAKDFIALSE EARKYIVRTT KTKGINFMLT FNDRRMHLTI 

      2530       2540       2550       2560       2570       2580 
PTISVANKKG AGLPSLFTRL YSFFWHLCVL IVVLFVATSL LDFSAQVTSD TQYDFKYIEN 

      2590       2600       2610       2620       2630       2640 
GVLKVFEKPL DCVHNAFVNF NEWHNAKFGS IPTNSRRCPI VVGTSDEVRY IPGVPAGVFL 

      2650       2660       2670       2680       2690       2700 
YGKSLIFAMS TIFGTSGLCF DDRGLTDPDS CIFNSACTTL SGIGGRNVYC YREGVVDNAK 

      2710       2720       2730       2740       2750       2760 
LYSSLLPHSY YRLMDGNHIV LPEIITRGFG IRTIKTQAMT YCRTGECIDS QAGVCVGLDR 

      2770       2780       2790       2800       2810       2820 
FFVYSKTPGS DYVCGTGFFS LLFNVIGMFS NSIPVTVMSG QILLNCVVAF TAVMACFAFT 

      2830       2840       2850       2860       2870       2880 
KFKRLFGDMS FGVLSVGLCT VVNNLSYVVT QNSIGMLAYA TLYFLCTKGV RYSWVWHVGF 

      2890       2900       2910       2920       2930       2940 
AISYCFLAPW WVVLAYLICA LLEFLPNLFK LKVSTQLFEG DKFVGSFESA ASGTFVLDMH 

      2950       2960       2970       2980       2990       3000 
SYQKLANSIS TEKLKQYCAS YNRYKYYSGS ASEADYRLAC FAHLAKAMSD FANDHMDKLY 

      3010       3020       3030       3040       3050       3060 
TPPTVSYNST LQAGLRKMAQ PSGIVEGCIV RVSYGNLTLN GLWLGDTVIC PRHVIASNTT 

      3070       3080       3090       3100       3110       3120 
NVIDYDHAMS LVRLHNFSIS SGNMFLGVIS ASMRGTLLHI KVNQSNVNTP NYTYKVLKPG 

      3130       3140       3150       3160       3170       3180 
DSFNILACYD GSAAGVYGVN MRTNYTIRGS FISGACGSPG YNINNGVVEF CYMHHLELGS 

      3190       3200       3210       3220       3230       3240 
GCHVGSDMDG TMYGKYEDQP TLQIEGASNL VTENVCSWLY GALINGDRWW LSSVSVGVDT 

      3250       3260       3270       3280       3290       3300 
YNEWALRNGM TALKNVDCFS LLVAKTGVDV GRLLASIQKL HGNFGGKSIL GCTSLCDEFT 

      3310       3320       3330       3340       3350       3360 
LSEVVKQMYG VTLQSGKVSR AFRNASIVCC LLFLFLSEML NHSKLFWINP GYITPVFLAI 

      3370       3380       3390       3400       3410       3420 
IVASSALMLL VKHKLLFLQL YLLPSLCIVS GYNIFKDYHF YTYMLEEFDY KVPFGGFNVT 

      3430       3440       3450       3460       3470       3480 
GVLNISLCCF VMGLHTFRFL QTPNKIFSYV VAVLTVLYTY YYSTDVLGLI LTSMSGFTNY 

      3490       3500       3510       3520       3530       3540 
WFIGTATYKL ATYVLPHTSL LDSFDAIKAV VFLYLLLGYC NCVYYGSLYW INRFCKLTLG 

      3550       3560       3570       3580       3590       3600 
CYEFKVSAAE FKYMVANGLR APTGVFDALI LSLKLIGVGG RKTIKISSVQ SKLTDLKCTN 

      3610       3620       3630       3640       3650       3660 
VVLLGCLSNM NIAANSREWA YCVDLHNKIN LCNDAEAAQE MLLALLAFFL SKNSAFGVDE 

      3670       3680       3690       3700       3710       3720 
LLDSYFNDSS VLQSVAATYV NLPSYLAYET ARQSYEDALA NGSPPQLVKQ LRHAMNVAKS 

      3730       3740       3750       3760       3770       3780 
EFDREASTQR KLDRMAEQAA SQMYKEARAV NRKSKVVSAM HSLLFGMLRR LDMSSVDTIL 

      3790       3800       3810       3820       3830       3840 
SLAKDGVVPL SIIPAVSATK LNIVVSDIES YSKIQREGCV HYAGVIWSVV DIKDNDGKPV 

      3850       3860       3870       3880       3890       3900 
HAKEVVTSNV ESLAWPLFLN CERIIKLQNN EIIPSKIKQR PIKAEGEGVV ADGNALYSNE 

      3910       3920       3930       3940       3950       3960 
GGRTFMYAFI SDKPDLKVVK WEFDGGSNAI ELEPPCKFLV EAPSGPVVKY LYFVRNLNNL 

      3970       3980       3990       4000       4010       4020 
RRGAVLGFIG ATVRLQAGKQ TEQATNSSLL TLCAFAVDPP KTYLDAVKSG HRPVGNCVKM 

      4030       4040       4050       4060       4070       4080 
LANGSGNGQA ITNGVEASTN QDSYGGASVC LYCRAHVEHP DMDGFCKLRG KYVQVPLGTL 

      4090       4100       4110       4120       4130       4140 
DPIRFVLENT VCKVCGCWQA NGCTCDRAVI QSVDSGYLNR VRGSSAARLE PLNGSDTHHV 

      4150       4160       4170       4180       4190       4200 
FRAFDVYNRD VACISKFLKV NCVRLKNLDK HDAFWIVKKC TKSVMEHEQS IYNLISDCGA 

      4210       4220       4230       4240       4250       4260 
VAKHDFFTWK EGRSVYGNVC RQDLTEYTMM DLCYALRNFD ENNCETLKKI LVVVGACDES 

      4270       4280       4290       4300       4310       4320 
YFDNKLWFDP VENEDVHRVY AKLGTIVARA MLKCVKYCDA MVEQGIVGVI TLDNQDLNGD 

      4330       4340       4350       4360       4370       4380 
FYDFGDFVTS VKGMGVPICT SYYSYMMPVM GMTNCLASEC FIKSDIFGED FRTFDLLAYD 

      4390       4400       4410       4420       4430       4440 
FTEHKVNLFN KYFKHWGQTY HPNCEDCHDE SCIVHCANFN TLFATTIPIT AFGPLCRKCW 

      4450       4460       4470       4480       4490       4500 
IDGVPLVTTA GYHFKQLGIV WNKDLNLHSS RLTINELLQF CADPSLLIAS SPALVDKRTV 

      4510       4520       4530       4540       4550       4560 
CFSVAALGTG MTNQTVKPGH FNREFYDFLR SQGFFEEGSE LTLKHFFFAQ KGDAAVRDFD 

      4570       4580       4590       4600       4610       4620 
YYRYNRTTVL DICQARVVYQ IVQCYFGMYE GGCITAKEVI VNNLNKSAGY PFNKFGKAGL 

      4630       4640       4650       4660       4670       4680 
YYDSLSYEEQ DDLYAYTKRN IIPTMTQLNL KYAISGKDRA RTVGGVSLLS TMTTRQYHQK 

      4690       4700       4710       4720       4730       4740 
HLKSIVNTRG ASVVIGTTKF YGGWDNMLKT LIKDVENPHL MGWDYPKCDR ALPNMIRMIS 

      4750       4760       4770       4780       4790       4800 
AMILGSKHVN CCSSSDRYYR LCNELAQVLT EMVYSNGGFY VKPGGTTSGD ATTAYANSVF 

      4810       4820       4830       4840       4850       4860 
NIFQATSANV NRLLSVDSNT CNNIEVKQLQ RKLYDCCYRS SSVDQSFVEE YFGYLRKHFS 

      4870       4880       4890       4900       4910       4920 
MMILSDDGVV CYNSEYAALG YVADLNAFKA VLYYQNNVFM SASKCWIEPD INKGPHEFCS 

      4930       4940       4950       4960       4970       4980 
QHTMQIVDKD GTYYLPYPDP SRILSAGVFV DDIVKTDPVI LLERYVSLAI DAYPLSKHDN 

      4990       5000       5010       5020       5030       5040 
PEYRRVFTVM LDWVKHLYKT LNQGVLDSFS VTLLEDATAK FWDESFYASM YEQSSVLQSA 

      5050       5060       5070       5080       5090       5100 
GLCVVCSSQT VLRCGDCIRR PMLCTKCAYD HVVSTSHKFI LAITPYVCCS SGCGVSDVTK 

      5110       5120       5130       5140       5150       5160 
LYLGGLSYWC VDHKPRLSFP LCSSGNVFGL YKNSATGSPD VDDFNTLATS DWTDVKDYKL 

      5170       5180       5190       5200       5210       5220 
ANDVKDSLRL FAAETIKAKE ESVKSSYACA TIHEVVGPKE LVLKWEVGKP RPPLSRNSVF 

      5230       5240       5250       5260       5270       5280 
TCYHITKNTK FQVGEFTFEK LDYDNDAVSY KSTATTKLVP GMVFVLTSHN VQPLRAPTII 

      5290       5300       5310       5320       5330       5340 
NQERYSTLHK LRPAFNIHED YSNLIPYYQL IGKQKLTTIQ GPPGSGKSHC VIGLGLYFPG 

      5350       5360       5370       5380       5390       5400 
ARIVFTACSH AAVDSLCVKA ATAYSSDRCS RIIPQKARIE CYDGFKSNNT SAQYLFSTVN 

      5410       5420       5430       5440       5450       5460 
ALPEVNADIC VVDEVSMCTN YDLSVINQRV NYRHIVYVGD PQQLPAPRVM ITRGVLVPED 

      5470       5480       5490       5500       5510       5520 
YNVVTRRMCV LKPDIFLHKC YRCPAEIVNT VSEMVYENQF VPVKSESKEC FKIYCRGNVQ 

      5530       5540       5550       5560       5570       5580 
VDNGSSINRR QLEVVRMFLA KNPKWAKAVF ISPYNSQNYV AGRVLGLQIQ TVDSSQGSEY 

      5590       5600       5610       5620       5630       5640 
DYVIYTQTSD TAHASNVNRF NVAITRAKKG ILCIMCDREL FDILKFYELK LSDLQVGDGC 

      5650       5660       5670       5680       5690       5700 
GLFKDCYKGE DNLPPSHAPT FMSLSDNFKT DKDLAVQIGV NGPVKYEHVI SFMGFRFDIN 

      5710       5720       5730       5740       5750       5760 
VPNQHTLFCT RDFAMRNARG WLGFDVEGAH VIGSNVGTNV PLQLGFSNGV DFVVRPEGCV 

      5770       5780       5790       5800       5810       5820 
STEVGDVIQP VRARAPPGDQ FTHLLPLLRK GQPWSVIRRR IVQMCSDYLA NLSDTLIFVL 

      5830       5840       5850       5860       5870       5880 
WSGGLELTTM RYFVKLGPVQ TCDCGKRATC YNSTNHTFSC FRHALGSDYI YNCYCIDIQQ 

      5890       5900       5910       5920       5930       5940 
WGYTGSLSMN HHEVCNIHRN EHVASGDAAM TRCLAIHDCF VKNVDWSITY PFIANEQAIN 

      5950       5960       5970       5980       5990       6000 
KSGRLVQSHV MRAVLKLYNP KAIHDVGNPK GIRCVVTDAS WYCYDKNPTN TNVKMLEYDY 

      6010       6020       6030       6040       6050       6060 
ITHGQLDGLC LFWNCNVDMY PEFSVVCRFD TRMRSTLNLE GCNGGSLYVN NHAFHTPAYD 

      6070       6080       6090       6100       6110       6120 
KRAFAKLKAM PFFFYDDSEC EKLQDAVNYV PLRASNCITR CNVGGAVCSK HCALYHNYVM 

      6130       6140       6150       6160       6170       6180 
AYNTFTTAGF TIWVPNSFDM FNLWQTFKNS NVQGLENIAY NVVKKGSFVG VEGELPVAVV 

      6190       6200       6210       6220       6230       6240 
NDKVMVRDGV SDNVVFVNNT SLPTNVAFEL YAKRKVGLTP PLTILKNLGV VCTSKCVLWD 

      6250       6260       6270       6280       6290       6300 
YEASRPLTTF TKDVCKYTDF DGDVCTLFDN SVPGAFERFT VTKNAVLISL TAVKKLTAIK 

      6310       6320       6330       6340       6350       6360 
LTYGYLNGVP VFTHEDKPFT WYIYTRKDGA FVEYPDGYFT QGRVISDFQP RSNMEEDFLN 

      6370       6380       6390       6400       6410       6420 
MDMGLFISKY GLEDYGFEHV VFGDVSKTTL GGLHLLISQI RLSKIGVLKV EDFVSSSDST 

      6430       6440       6450       6460       6470       6480 
LKSCTVTYVD NPSSKMVCTY VDLLLDDFVN ILKSVDLSVV SKVHEVVIDC KVWRWMLWCK 

      6490       6500       6510       6520       6530       6540 
DHKVQTFYPQ LQSAEWKCGY SMPSIYKIQR MCLEPCNLYN YGSGLKLPDG IMFNVVKYTQ 

      6550       6560       6570       6580       6590       6600 
LCQYLNSTTM CVPHHMRVLH LGAGSDKGVA PGTAVLRRWL PLDAVIVDND VNDYVSDADF 

      6610       6620       6630       6640       6650       6660 
SYTGDCASMY LTDKFDLVIS DMYDGRTKSC DGDNVSKEGF FPYINGVITE KLALGGTVAI 

      6670       6680       6690       6700       6710       6720 
KITEFSWNKK LYELIQKFEY WTLFCTSVNT SSSEAFLIGV HFLGDFSTNA IIDGNIMHAN 

      6730       6740       6750       6760       6770       6780 
YIFWRNSTIM TMSYNSVLDL SKFSCKHKAT VVVNLKDSSV TDLVLGLLKN GKLLIRNNGV 

      6790 
VCGFSNHLVN STK 

« Hide

Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See P0C6F6.

References

[1]"Prevalence and genetic diversity of coronaviruses in bats from China."
Tang X.C., Zhang J.X., Zhang S.Y., Wang P., Fan X.H., Li L.F., Li G., Dong B.Q., Liu W., Cheung C.L., Xu K.M., Song W.J., Vijaykrishna D., Poon L.L.M., Peiris J.S.M., Smith G.J., Chen H., Guan Y.
J. Virol. 80:7481-7490(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ648858 Genomic RNA. Translation: ABG47077.1.
RefSeqYP_001351683.1. NC_009657.1. [P0C6W0-1]

3D structure databases

ProteinModelPortalP0C6W0.
SMRP0C6W0. Positions 3013-3311, 3871-3975, 3982-4107.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0C6W0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11266518.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_BC512
AccessionPrimary (citable) accession number: P0C6W0
Secondary accession number(s): Q0Q467
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries