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P0C6V9

- R1AB_BC279

UniProt

P0C6V9 - R1AB_BC279

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Protein
Replicase polyprotein 1ab
Gene
rep, 1a-1b
Organism
Bat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.
The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium By similarity.
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.
Nsp9 is a ssRNA-binding protein By similarity.
NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1802Cleavage By similarity
Sitei818 – 8192Cleavage; by PL-PRO By similarity
Active sitei1657 – 16571For PL-PRO activity By similarity
Active sitei1818 – 18181For PL-PRO activity By similarity
Sitei3246 – 32472Cleavage; by PL-PRO By similarity
Active sitei3287 – 32871For 3CL-PRO activity By similarity
Active sitei3391 – 33911For 3CL-PRO activity By similarity
Sitei3552 – 35532Cleavage; by 3CL-PRO By similarity
Sitei3842 – 38432Cleavage; by 3CL-PRO By similarity
Sitei3925 – 39262Cleavage; by 3CL-PRO By similarity
Sitei4123 – 41242Cleavage; by 3CL-PRO By similarity
Sitei4236 – 42372Cleavage; by 3CL-PRO By similarity
Sitei4375 – 43762Cleavage; by 3CL-PRO By similarity
Sitei5307 – 53082Cleavage; by 3CL-PRO By similarity
Sitei5908 – 59092Cleavage; by 3CL-PRO By similarity
Sitei6435 – 64362Cleavage; by 3CL-PRO By similarity
Sitei6781 – 67822Cleavage; by 3CL-PRO By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1735 – 177238C4-type By similarity
Add
BLAST
Zinc fingeri4310 – 432617 By similarity
Add
BLAST
Zinc fingeri4353 – 436614 By similarity
Add
BLAST
Nucleotide bindingi5589 – 55968ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. endonuclease activity Source: UniProtKB-KW
  6. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  7. helicase activity Source: UniProtKB-KW
  8. methyltransferase activity Source: InterPro
  9. omega peptidase activity Source: InterPro
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  5. suppression by virus of host translation Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral RNA genome replication Source: InterPro
  9. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Exoribonuclease (EC:3.1.13.-)
Short name:
ExoN
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)
Taxonomic identifieri389167 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
ProteomesiUP000006573: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Reviewed prediction
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2209 – 222921Helical; Reviewed prediction
Add
BLAST
Transmembranei2310 – 233021Helical; Reviewed prediction
Add
BLAST
Transmembranei2357 – 237721Helical; Reviewed prediction
Add
BLAST
Transmembranei2761 – 278121Helical; Reviewed prediction
Add
BLAST
Transmembranei2998 – 301821Helical; Reviewed prediction
Add
BLAST
Transmembranei3028 – 304821Helical; Reviewed prediction
Add
BLAST
Transmembranei3060 – 308021Helical; Reviewed prediction
Add
BLAST
Transmembranei3083 – 310321Helical; Reviewed prediction
Add
BLAST
Transmembranei3111 – 313121Helical; Reviewed prediction
Add
BLAST
Transmembranei3148 – 316821Helical; Reviewed prediction
Add
BLAST
Transmembranei3570 – 359021Helical; Reviewed prediction
Add
BLAST
Transmembranei3592 – 361221Helical; Reviewed prediction
Add
BLAST
Transmembranei3618 – 363821Helical; Reviewed prediction
Add
BLAST
Transmembranei3665 – 368420Helical; Reviewed prediction
Add
BLAST
Transmembranei3691 – 371020Helical; Reviewed prediction
Add
BLAST
Transmembranei3734 – 375421Helical; Reviewed prediction
Add
BLAST
Transmembranei3762 – 378221Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
  2. host cell membrane Source: UniProtKB-SubCell
  3. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Non-structural protein 1 By similarity
PRO_0000289896Add
BLAST
Chaini180 – 818639Non-structural protein 2 By similarity
PRO_0000289897Add
BLAST
Chaini819 – 27461928Non-structural protein 3 By similarity
PRO_0000289898Add
BLAST
Chaini2747 – 3246500Non-structural protein 4 By similarity
PRO_0000289899Add
BLAST
Chaini3247 – 35523063C-like proteinase By similarity
PRO_0000289900Add
BLAST
Chaini3553 – 3842290Non-structural protein 6 By similarity
PRO_0000289901Add
BLAST
Chaini3843 – 392583Non-structural protein 7 By similarity
PRO_0000289902Add
BLAST
Chaini3926 – 4123198Non-structural protein 8 By similarity
PRO_0000289903Add
BLAST
Chaini4124 – 4236113Non-structural protein 9 By similarity
PRO_0000289904Add
BLAST
Chaini4237 – 4375139Non-structural protein 10 By similarity
PRO_0000289905Add
BLAST
Chaini4376 – 5307932RNA-directed RNA polymerase By similarity
PRO_0000289906Add
BLAST
Chaini5308 – 5908601Helicase By similarity
PRO_0000289907Add
BLAST
Chaini5909 – 6435527Exoribonuclease By similarity
PRO_0000289908Add
BLAST
Chaini6436 – 6781346Uridylate-specific endoribonuclease By similarity
PRO_0000289909Add
BLAST
Chaini6782 – 7079298Putative 2'-O-methyl transferase By similarity
PRO_0000289910Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6V9.
SMRiP0C6V9. Positions 13-127, 819-930, 1000-1174, 1337-1475, 1547-1860, 3247-3552, 3842-3925, 3927-4117, 4124-4236, 4246-4368, 6436-6779.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1001 – 1167167Macro
Add
BLAST
Domaini1617 – 1881265Peptidase C16
Add
BLAST
Domaini3247 – 3552306Peptidase C30
Add
BLAST
Domaini4987 – 5149163RdRp catalytic
Add
BLAST
Domaini5308 – 539184CV MBD
Add
BLAST
Domaini5564 – 5745182(+)RNA virus helicase ATP-binding
Add
BLAST
Domaini5746 – 5915170(+)RNA virus helicase C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2098 – 2377280HD1 By similarity
Add
BLAST
Regioni2761 – 3168408HD2 By similarity
Add
BLAST
Regioni3570 – 3782213HD3 By similarity
Add
BLAST
Regioni4918 – 5220303RNA-directed RNA polymerase By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi930 – 99970Glu-rich
Add
BLAST
Compositional biasi2216 – 22194Poly-Leu
Compositional biasi3772 – 37754Poly-Cys
Compositional biasi5312 – 533726Cys-rich
Add
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6V9-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MESLALGVSE KTHVQLSLPV LQVRDVLVRG FGDSVEEALA EAREHLKNGT     50
CGLVELEKGV LPQLEQPYVF IKRSDAQGTN HGYKVVELVA ELDGIQYGRS 100
GTTLGVLVPH VGETPVAYRN VLLRKNGNKG AGGHSYGIDL KSYDLGVELG 150
TDPIEDYEQN WNTKHGGGVL RELIRELNGG AFTRYVDNNF CGPDGYPLEC 200
IKDLLARAGK SMCTLSEQLD YIESKRGVYC CREHEHEIVW FTERSEKSYE 250
RQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI 300
RSVYPVATPQ ECNDMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV 350
CEGPTTCGYL PANAVVKMPC PACQDPEVGP EHSVADYHNH SNIETRLRKG 400
GRTKCFGGCV FAYVGCYNKR AYWVPRASAN IGASHTGITG DNVETLNEDL 450
MEILNRDRVN INIVGDFHLN EEVAIILASF SASTCAFVDT VKGLDYKTFK 500
DIVESCGNFK VTRGRAKKGA WNIGQEKSIL TPLYGFPSQA AGVIRSIFTR 550
ALDTANHSIP DLQRAAITIL DGISEQSLRL IDAMVYTSDL LTNSVIVMAY 600
VTGGLVQQIT QWLSNMLGTT VDKLKPVFTW VEAKLSAGIE FLRDAWEILK 650
FLVTGVFDIV KGQIQVASDN LKECVKAFLD VLNKALEMCI DQVIIAGAKL 700
RTLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLRAPKEVT FFEGDSHDTV 750
FTSEEVVLKN GELEALETPV DSFTNGAVIG TPVCVNGLML LELKDKEQYC 800
ALSPGLLATN NVFSLKGGAP VKGVTFGEDT VLEVQGYKNV KITFELDERV 850
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE 900
WSVATFYLFD DAGEEKLSSR MYCSFYPPDE EEDCEEYEDE EEIPEETCEH 950
EYGTEDDYKG LPLEFGASTE IQQVDEEEEE DWLEEAIAAK PEPEPLPEEP 1000
VNQFTGYLKL TDNVAIKCVD IVKEAQHAKP TVIVNAANVH LKHGGGVAGA 1050
LNKATNGAMQ QESDDYIKKN GPLTVGGSCL LSGHNLAKKC MHVVGPNLNA 1100
GEDVQLLKAA YANFNSQDVL LAPLLSAGIF GAKPLQSLKM CVETVRTQVY 1150
FAVNDQDLYD HVVLGYLDSL KPKVETPTQE NLELKEQPAV ETLTQENLEL 1200
EELPVIEKPV DVKFKARIEE VNTSLEETKF LTSRLLLFAD INGKLYQDSQ 1250
NMLRGEDMFF LEKDAPYIVG DVISSGDITC VIIPAKKAGG TTEMLAKALK 1300
KVPVSEYITT YPGQGCAGYT LEEAKTALRK CKSVFYVLPS KTPNDKEEIL 1350
GTVSWNLREM LAHAEETRKL MLICMDVKAL MSTIHRRYKG IKVQEGIVDY 1400
GVRFFFYTSK EPVASIITKL NLLNEPLVTM PIGYVTHGLN LEEAARCMRS 1450
LKAPAVVSVS SPDAVTTYNG YLTSSSKTSE EHFIETVSLA GMYRDWSYSG 1500
QRTELGVEFL KRGDKVVYHT VGSPIQFHLD GEVLLLDKLK SLLSLREVRT 1550
IKVFTTVDNT NLHTQIVDMS MTYGQQFGPT YLDGADVTKI KPHAKHEGKT 1600
FFVLPSDDTL RSEAFEYYHT LDESFLGRYM SALNHTKKWK FPQIGGLTSI 1650
KWADNNCYLS SVLLALQQIE VKFNAPALQE AYYRARAGDA ANFCALILAY 1700
SNRTVGELGD VRETMTHLLQ HANLESAKRV LNVVCKTCGQ KSTTLTGVEA 1750
VMYMGTLSYE ELKTGVTIPC ICGRDATQYL VQQESSFVMM SAPPSEYTLQ 1800
QGAFLCANEY TGSYQCGHYT HVTVKETLYR IDGAYLTKMS EYKGPVTDVF 1850
YKEISYTTTI KPVSYKLDGV IYTEIQPKLD EYYKKDNAYY TEQPIDLVPT 1900
QPLPNASFDN FKLTCSNTKF ADDLNQMTGF KKPASRELSV TFFPDLNGDV 1950
VAIDYRHYSA SFKKGAKLLH KPIIWHINQT TNKTTYKPNT WCLRCLWSTK 2000
PVETSNSFEV LEVEDTQGMD NLACESQTPT SEEVVENPTI QKEVIECDVK 2050
TIEVVGNVIL KPSEEGVKVT QELGHEDLMA AYVEETSITI KKPNELSLAL 2100
GLRTLATHGA AAINSVPWSK ILAYVKPFLG QAAVTTTNCI KRCVQRVFNN 2150
YMPYVITLLF QLCTFTRSTN SRIRASLPTT IAKNSVKSVA KLCLDVCINY 2200
VKSPKFSKLF TIAMWLLLLS ICLGSLIYVT AAFGVLLSNL GIPSYCDGVR 2250
ESYVNSSNVT TMDFCEGSFL CSVCLNGLDS LDSYPALETI QVTISSYKLD 2300
LTSLGLAAEW FLAYMLFTKF FYLLGLSAIM QVFFGYFASH FISNSWLMWF 2350
IISIVQMAPV SAMVRMYIFF AFCYYVWKSY VHIMDGCTSS TCMMCYKRNR 2400
ATRVECTTIV NGMKRSFYVY ANGGRGFCKA HNWNCLNCDT FCAGSTFISD 2450
EVARDLSLQF KRPINPTDQS SYVVDSVAVK NGALHLYFDK AGQKTYERHP 2500
LSHFVNLDNL RANNTKGSLP INVIVFDGKS KCDESAAKSA SVYYSQLMCQ 2550
PILLLDQALV SDVGDSTEVS VKMFDAYVDT FSATFSVPME KLKALVATAH 2600
SELAKGVALD GVLSTFVSAA RQGVVDTDVD TKDVIECLKL SHHSDLEVTG 2650
DSCNNFMLTY NKVENMTPRD LGACIDCNAR HINAQVAKSH NVSLIWNVKD 2700
YMSLSEQLRK QIRSAAKKNN IPFRLTCATT RQVVNAITTK ISLKGGKIVS 2750
TWFKLMLKAT LLCVLAALFC YIIMPVHSLS VHDGYTNEII GYKAIQDGVT 2800
RDIMATDDCF ANKHAGFDSW FSQRGGSYRN DKSCPVVAAI ITREIGFIVP 2850
GLPGTVLRAI NGDFLHFLPR VFSAVGNICY TPSKLIEYSD FATSACVLAA 2900
ECTIFKDAMG KPVPYCYDTN LLEGSISYSE LRPDTRYVLM DGSIIQFPNT 2950
YLEGSVRVVT TFDAEYCRHG TCERSEAGVC LSTSGRWVLN NEHYRALPGV 3000
FCGVDAMNLI ANIFTPLVQP VGALDVSASV VAGGIIAILV TCAAYYFMKF 3050
RRAFGEYNHV VAANALLFLM SFTILCLAPA YSFLPGVYSI FYLYLTFYFT 3100
NDVSFLAHLQ WFAMFSPIVP FWITAIYVFC ISLKHCHWFF NNYLRKRVMF 3150
NGVTFSTFEE AALCTFLLNK EMYLKLRSET LLPLTQYNRY LALYNKYKYF 3200
SGALDTTSYR EAACCHLAKA LNDFSNSGAD VLYQPPQTSI TSAVLQSGFR 3250
KMAFPSGKVE GCMVQVTCGT TTLNGLWLDD TVYCPRHVIC TAEDMLNPNY 3300
EDLLIRKSNH SFLVQAGNVQ LRVIGHSMQN CLLRLKVDTS NPKTPKYKFV 3350
RIQPGQTFSV LACYNGSPSG VYQCAMRPNY TIKGSFLNGS CGSVGFNIDY 3400
DCVSFCYMHH MELPTGVHAG TDLEGKFYGP FVDRQTAQAA GTDTTITLNV 3450
LAWLYAAVIN GDRWFLNRFT TTLNDFNLVA MKYNYEPLTQ DHVDILGPLS 3500
AQTGIAVLDM CAALKELLQN GMNGRTILGS TILEDEFTPF DVVRQCSGVT 3550
FQGKFKKIVK GTHHWMLLTF LTSLLILVQS TQWSLFFFVY ENAFLPFTLG 3600
IMAIAACAML LVKHKHAFLC LFLLPSLATV AYFNMVYMPA SWVMRIMTWL 3650
ELADTSLSGY RLKDCVMYAS ALVLLVLMTA RTVYDDAARR VWTLMNVITL 3700
VYKVYYGNSL DQAISMWALV ISVTSNYSGV VTTIMFLARA IVFVCVEYYP 3750
LLFITGNTLQ CIMLVYCFLG YCCCCYFGLF CLLNRYFRLT LGVYDYLVST 3800
QEFRYMNSQG LLPPKSSIDA FKLNIKLLGI GGKPCIKVAT VQSKMSDVKC 3850
TSVVLLSVLQ QLRVESSSKL WAQCVQLHND ILLAKDTTEA FEKMVSLLSV 3900
LLSMQGAVDI NKLCEEMLDN RATLQAIASE FSSLPSYAAY ATAQEAYEQA 3950
VANGDSEVVL KKLKKSLNVA KSEFDRDAAM QRKLEKMADQ AMTQMYKQAR 4000
SEDKRAKVTS AMQTMLFTML RKLDNDALNN IINNARDGCV PLNIIPLTTA 4050
AKLMVVVPDY GTYKNTCDGN TFTYASALWE IQQVVDADSK IVQLSEINMD 4100
NSQNLAWPLI VTALRANSAV KLQNNELSPV ALRQMSCAAG TTQTACTDDN 4150
ALAYYNNSKG GRFVLALLSD HQDLKWARFP KSDGTGTIYT ELEPPCRFVT 4200
DTPRGPKVKY LYFIKGLNNL NRGMVLGSLA ATVRLQAGNA TEVPANSAVL 4250
SFCAFAVDPA KAYKDYLASG GQPITNCVKM LCTHTGTGQA ITVTPEANMD 4300
QESFGGASCC LYCRCHIDHP NPKGFCDLKG KYVQIPATCA NDPVGFTLKN 4350
TVCTVCGTWK GYGCSCDQLR EPMMQSADAS TFLNRVCGVS AARLTPCGTG 4400
TSTDVVYRAF DIYNERVAGF AKFLKTNCCR FQEKDEEGNL LDSYFVVKRH 4450
TMSNYQHEET IYNLVKECPA VAVHDFFKFR VDGDMVPHIS RQRLTKYTMA 4500
DLVYALRHFD EGNCDTLKEI LVTYNCCDDD YFNKKDWYDF VENPDILRVY 4550
ANLGERVRQA LLKTVQFCDA MRDAGIVGVL TLDNQDLNGN WYDFGDFVQV 4600
APGCGVPIVD SYYSLLMPIL TLTKALAAES HMDADLAKPL IKWDLLKYDF 4650
TEERLCLFDR YFKYWDQTYH PNCINCLDDR CILHCANFNV LFSTVFPPTS 4700
FGPLVRKIFV DGVPFVVSTG YHFRELGVVH NQDVNLHSSR LSFKELLVYA 4750
ADPAMHAASG NLLLDKRTTC FSVAALTNNV AFQTVKPGNF NKDFYDFAVS 4800
KGFFKEGSSV ELKHFFFAQD GNAAISDYDY YRYNLPTMCD IRQLLFVVEV 4850
VDKYFDCYDG GCINANQVIV NNLDKSAGFP FNKWGKARLY YDSMSYEDQD 4900
VLFAYTKRNV IPTITQMNLK YAISAKNRAR TVAGVSICST MTNRQFHQKL 4950
LKSIAATRGA TVVIGTSKFY GGWHNMLKTV YSDVETPHLM GWDYPKCDRA 5000
MPNMLRIMAS LVLARKHSTC CNLSHRFYRL ANECAQVLSE MVMCGGSLYV 5050
KPGGTSSGDA TTAYANSVFN ICQAVTANVN ALLSTDGNKI ADKYVRNLQH 5100
RLYECLYRNR DVDHEFVDEF YAYLRKHFSM MILSDDAVVC YNSNYAAQGL 5150
VASIKNFKAV HYYQNNVFMS EAKCWTETDL TKGPHEFCSQ HTMLVKQGDD 5200
YVYLPYPDPS RILGAGCFVD DIVKTDGTLM IERFVSLAID AYPLTKHPNQ 5250
EYADVFHLYL QYIRKLHDEL TGHMLDMYSV MLTNDNTSRY WEPEFYEAMY 5300
TPHTVLQAVG ACVLCNSQTS LRCGACIRRP FLCCKCCYDH VISTSHKLVL 5350
SVNPYVCNAP GCDVTDVTQL YLGGMSYYCK LHKPPISFPL CANGQVFGLY 5400
KNTCVGSDNV TDFNAIATCD WTNAGDYILA NTCTERLKLF AAETLKATEE 5450
TFKLSYGIAT VREVLSDREL HLSWEVGKPR PPLNRNYVFT GYRVTKNSKV 5500
QIGEYTFEKG DYGDAVVYRG TTTYKLNVGD YFVLTSHTVM PLSAPTLVPQ 5550
EHYVRITGLY PTLNISDEFS SNVANYQKVG MQKYSTLQGP PGTGKSHFAI 5600
GLALYYPSAR IVYTACSHAA VDALCEKALK YLPIDKCSRI IPARARVECF 5650
DKFKVNSTLE QYVFCTVNAL PETTADIVVF DEISMATNYD LSVVNARLRA 5700
KHYVYIGDPA QLPAPRTLLT KGTLEPEYFN SVCRLMKTIG PDMFLGTCRR 5750
CPAEIVDTVS ALVYDNKLKA HKEKSAQCFK MFYKGVITHD VSSAINRPQI 5800
GVVREFLTRN PAWRKAVFIS PYNSQNAVAS KILGLPTQTV DSSQGSEYDY 5850
VIFTQTTETA HSCNVNRFNV AITRAKIGIL CIMSDRDLYD KLQFTSLEVP 5900
RRNVATLQAE NVTGLFKDCS KIITGLHPTQ APTHLSVDTK FKTEGLCVDI 5950
PGIPKDMTYR RLISMMGFKM NYQVNGYPNM FITREEAIRH VRAWIGFDVE 6000
GCHATRDAVG TNLPLQLGFS TGVNLVAVPT GYVDTENNTE FTRVNAKPPP 6050
GDQFKHLIPL MYKGLPWNVV RIKIVQMLSD TLKGLSDRVV FVLWAHGFEL 6100
TSMKYFVKIG PERTCCLCDR RATCFSTSSD TYACWNHSVG FDYVYNPFMI 6150
DVQQWGFTGN LQSNHDQHCQ VHGNAHVASC DAIMTRCLAV HECFVKRVDW 6200
SVEYPIIGDE LKINAACRKV QHMVVKSALL ADKFSVLHDI GNPKAIKCVP 6250
QAEVDWKFYD AQPCSDKAYK IEELFYSYAT HHDKFTDGVC LFWNCNVDRY 6300
PANAIVCRFD TRVLSNLNLP GCDGGSLYVN KHAFHTPAFD KSAFTYLKQL 6350
PFFYYSDSPC ESHGKQVVSD IDYVPLKSAT CITRCNLGGA VCRRHANEYR 6400
QYLDAYNMMI SAGFSLWIYK QFDTYNLWNT FTRLQSLENV AYNVVNKGHF 6450
DGQIGEAPVS IINNAVYTKV DGNDVEIFEN KTTLPVNVAF ELWAKRNIKP 6500
VPEIKILNNL GVDIAANTVI WDYKREAPAH VSTIGVCTMT DIAKKPTESA 6550
CSSLTVLFDG RVEGQVDLFR NARNGVLITE GSVKGLTPSK GPAQASVNGV 6600
TLIGESVKTQ FNYFKKVDGI IQQLPETYFT QSRDLEDFKP RSKMETDFLE 6650
LAMDEFIQRY KLEGYAFEHI VYGDFSHGQL GGLHLMIGLA KRSQDSPLKL 6700
EDFIPTDSTV KNYFITDAQT GSSKCVCSVI DLLLDDFVEI IKSQDLSVIS 6750
KVVKVTIDYA EISFMLWCKD GHVETFYPKL QASQAWQPGV AMPNLYKMQR 6800
MLLEKCDLQN YGENAVIPKG IMMNVAKYTQ LCQYLNTLTL AVPYNMRVIH 6850
FGAGSDKGVA PGTAVLRQWL PTGALLVDSD LNDFVSDADS TLIGDCATVH 6900
TANKWDLIIS DMYDPKTKHV TKENDSKEGF FTYLCGFIKQ KLALGGSVAV 6950
KITEHSWNAD LYKLMGHFSW WTAFVTNVNA SSSEAFLIGV NYLGKLREQI 7000
DGYTMHANYI FWRNTNPIQL SSYSLFDMSK FPLKLRGTAV MSLKENQIND 7050
MIYSLLENGR LIIRENNRVV VSSDILVNN 7079

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,079
Mass (Da):791,654
Last modified:June 10, 2008 - v1
Checksum:iCE92AE7AE019B902
GO
Isoform Replicase polyprotein 1a (identifier: P0C6F5-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6F5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,388
Mass (Da):487,709
GO

Sequence cautioni

The sequence ABG47068.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648857 Genomic RNA. Translation: ABG47068.1. Different initiation.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648857 Genomic RNA. Translation: ABG47068.1 . Different initiation.

3D structure databases

ProteinModelPortali P0C6V9.
SMRi P0C6V9. Positions 13-127, 819-930, 1000-1174, 1337-1475, 1547-1860, 3247-3552, 3842-3925, 3927-4117, 4124-4236, 4246-4368, 6436-6779.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1AB_BC279
AccessioniPrimary (citable) accession number: P0C6V9
Secondary accession number(s): Q0Q476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus 279/2005 is highly similar to SARS-CoV (SARS-like).

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi