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P0C6V9

- R1AB_BC279

UniProt

P0C6V9 - R1AB_BC279

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Protein

Replicase polyprotein 1ab

Gene

rep

Organism
Bat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.By similarity
Host translation inhibitor nsp1: Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.By similarity
Non-structural protein 2: May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.By similarity
Papain-like proteinase: Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.By similarity
Non-structural protein 4: Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.By similarity
Proteinase 3CL-PRO: Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).By similarityPROSITE-ProRule annotation
Non-structural protein 6: Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.By similarity
Non-structural protein 7: Forms an hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity
Non-structural protein 8: Forms an hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.By similarity
Non-structural protein 9: May participate in viral replication by acting as a ssRNA-binding protein.By similarity
Non-structural protein 10: Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.By similarity
RNA-directed RNA polymerase: Responsible for replication and transcription of the viral RNA genome.By similarity
Helicase: Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.By similarity
Guanine-N7 methyltransferase: Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.By similarity
Uridylate-specific endoribonuclease: Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.By similarity
2'-O-methyltransferase: Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1802CleavageBy similarity
Sitei818 – 8192Cleavage; by PL-PROBy similarity
Active sitei1657 – 16571For PL-PRO activityPROSITE-ProRule annotation
Active sitei1818 – 18181For PL-PRO activityPROSITE-ProRule annotation
Sitei3246 – 32472Cleavage; by PL-PROBy similarity
Active sitei3287 – 32871For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3391 – 33911For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3552 – 35532Cleavage; by 3CL-PROBy similarity
Sitei3842 – 38432Cleavage; by 3CL-PROBy similarity
Sitei3925 – 39262Cleavage; by 3CL-PROBy similarity
Sitei4123 – 41242Cleavage; by 3CL-PROBy similarity
Sitei4236 – 42372Cleavage; by 3CL-PROBy similarity
Sitei4375 – 43762Cleavage; by 3CL-PROBy similarity
Sitei5307 – 53082Cleavage; by 3CL-PROBy similarity
Sitei5908 – 59092Cleavage; by 3CL-PROBy similarity
Sitei6435 – 64362Cleavage; by 3CL-PROBy similarity
Sitei6781 – 67822Cleavage; by 3CL-PROBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1735 – 177238C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4310 – 432617By similarityAdd
BLAST
Zinc fingeri4353 – 436614By similarityAdd
BLAST
Nucleotide bindingi5589 – 55968ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. methyltransferase activity Source: InterPro
  7. omega peptidase activity Source: InterPro
  8. RNA binding Source: UniProtKB-KW
  9. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host gene expression Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. viral protein processing Source: InterPro
  9. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Helicase, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host ISG15 by virus, Inhibition of host NF-kappa-B by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion, Viral RNA replication

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1ab
Short name:
pp1ab
Alternative name(s):
ORF1ab polyprotein
Cleaved into the following 15 chains:
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Papain-like proteinase (EC:3.4.19.12, EC:3.4.22.69)
Short name:
PL-PRO
Alternative name(s):
Non-structural protein 3
Short name:
nsp3
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
RNA-directed RNA polymerase (EC:2.7.7.48)
Short name:
Pol
Short name:
RdRp
Alternative name(s):
nsp12
Helicase (EC:3.6.4.12, EC:3.6.4.13)
Short name:
Hel
Alternative name(s):
nsp13
Alternative name(s):
nsp14
Alternative name(s):
NendoU
nsp15
Alternative name(s):
nsp16
Gene namesi
Name:rep
ORF Names:1a-1b
OrganismiBat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)
Taxonomic identifieri389167 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
ProteomesiUP000006573: Genome

Subcellular locationi

Chain Papain-like proteinase : Host membrane; Multi-pass membrane protein. Host cytoplasm By similarity
Chain Non-structural protein 4 : Host membrane; Multi-pass membrane protein. Host cytoplasm
Note: Localizes in virally-induced cytoplasmic double-membrane vesicles.By similarity
Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Helicase : Host endoplasmic reticulum-Golgi intermediate compartment Curated
Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2209 – 222921HelicalSequence AnalysisAdd
BLAST
Transmembranei2310 – 233021HelicalSequence AnalysisAdd
BLAST
Transmembranei2357 – 237721HelicalSequence AnalysisAdd
BLAST
Transmembranei2761 – 278121HelicalSequence AnalysisAdd
BLAST
Transmembranei2998 – 301821HelicalSequence AnalysisAdd
BLAST
Transmembranei3028 – 304821HelicalSequence AnalysisAdd
BLAST
Transmembranei3060 – 308021HelicalSequence AnalysisAdd
BLAST
Transmembranei3083 – 310321HelicalSequence AnalysisAdd
BLAST
Transmembranei3111 – 313121HelicalSequence AnalysisAdd
BLAST
Transmembranei3148 – 316821HelicalSequence AnalysisAdd
BLAST
Transmembranei3570 – 359021HelicalSequence AnalysisAdd
BLAST
Transmembranei3592 – 361221HelicalSequence AnalysisAdd
BLAST
Transmembranei3618 – 363821HelicalSequence AnalysisAdd
BLAST
Transmembranei3665 – 368420HelicalSequence AnalysisAdd
BLAST
Transmembranei3691 – 371020HelicalSequence AnalysisAdd
BLAST
Transmembranei3734 – 375421HelicalSequence AnalysisAdd
BLAST
Transmembranei3762 – 378221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Host translation inhibitor nsp1By similarityPRO_0000289896Add
BLAST
Chaini180 – 818639Non-structural protein 2By similarityPRO_0000289897Add
BLAST
Chaini819 – 27461928Papain-like proteinaseBy similarityPRO_0000289898Add
BLAST
Chaini2747 – 3246500Non-structural protein 4By similarityPRO_0000289899Add
BLAST
Chaini3247 – 35523063C-like proteinaseBy similarityPRO_0000289900Add
BLAST
Chaini3553 – 3842290Non-structural protein 6By similarityPRO_0000289901Add
BLAST
Chaini3843 – 392583Non-structural protein 7By similarityPRO_0000289902Add
BLAST
Chaini3926 – 4123198Non-structural protein 8By similarityPRO_0000289903Add
BLAST
Chaini4124 – 4236113Non-structural protein 9By similarityPRO_0000289904Add
BLAST
Chaini4237 – 4375139Non-structural protein 10By similarityPRO_0000289905Add
BLAST
Chaini4376 – 5307932RNA-directed RNA polymeraseBy similarityPRO_0000289906Add
BLAST
Chaini5308 – 5908601HelicaseBy similarityPRO_0000289907Add
BLAST
Chaini5909 – 6435527Guanine-N7 methyltransferaseBy similarityPRO_0000289908Add
BLAST
Chaini6436 – 6781346Uridylate-specific endoribonucleaseBy similarityPRO_0000289909Add
BLAST
Chaini6782 – 70792982'-O-methyltransferaseBy similarityPRO_0000289910Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Interactioni

Subunit structurei

Nsp2 interacts with host PHB and PHB2. 3CL-PRO exists as monomer and homodimer. Nsp4 interacts with PL-PRO and nsp6. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer dsRNA-encircling ring structure. Nsp9 is a dimer. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities. Nsp14 interacts (via N-terminus) with DDX1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6V9.
SMRiP0C6V9. Positions 13-127, 819-930, 1000-1174, 1337-1475, 1547-1860, 3247-3552, 3842-3925, 3927-4117, 4124-4236, 4246-4368, 6436-6779.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1001 – 1167167MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1617 – 1881265Peptidase C16PROSITE-ProRule annotationAdd
BLAST
Domaini3247 – 3552306Peptidase C30PROSITE-ProRule annotationAdd
BLAST
Domaini4987 – 5149163RdRp catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini5308 – 539184CV MBDAdd
BLAST
Domaini5564 – 5745182(+)RNA virus helicase ATP-bindingAdd
BLAST
Domaini5746 – 5915170(+)RNA virus helicase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2098 – 2377280HD1By similarityAdd
BLAST
Regioni2761 – 3168408HD2By similarityAdd
BLAST
Regioni3570 – 3782213HD3By similarityAdd
BLAST
Regioni4918 – 5220303RNA-directed RNA polymeraseBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi930 – 99970Glu-richAdd
BLAST
Compositional biasi2216 – 22194Poly-Leu
Compositional biasi3772 – 37754Poly-Cys
Compositional biasi5312 – 533726Cys-richAdd
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1735 – 177238C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4310 – 432617By similarityAdd
BLAST
Zinc fingeri4353 – 436614By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1ab (identifier: P0C6V9-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MESLALGVSE KTHVQLSLPV LQVRDVLVRG FGDSVEEALA EAREHLKNGT
60 70 80 90 100
CGLVELEKGV LPQLEQPYVF IKRSDAQGTN HGYKVVELVA ELDGIQYGRS
110 120 130 140 150
GTTLGVLVPH VGETPVAYRN VLLRKNGNKG AGGHSYGIDL KSYDLGVELG
160 170 180 190 200
TDPIEDYEQN WNTKHGGGVL RELIRELNGG AFTRYVDNNF CGPDGYPLEC
210 220 230 240 250
IKDLLARAGK SMCTLSEQLD YIESKRGVYC CREHEHEIVW FTERSEKSYE
260 270 280 290 300
RQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI
310 320 330 340 350
RSVYPVATPQ ECNDMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV
360 370 380 390 400
CEGPTTCGYL PANAVVKMPC PACQDPEVGP EHSVADYHNH SNIETRLRKG
410 420 430 440 450
GRTKCFGGCV FAYVGCYNKR AYWVPRASAN IGASHTGITG DNVETLNEDL
460 470 480 490 500
MEILNRDRVN INIVGDFHLN EEVAIILASF SASTCAFVDT VKGLDYKTFK
510 520 530 540 550
DIVESCGNFK VTRGRAKKGA WNIGQEKSIL TPLYGFPSQA AGVIRSIFTR
560 570 580 590 600
ALDTANHSIP DLQRAAITIL DGISEQSLRL IDAMVYTSDL LTNSVIVMAY
610 620 630 640 650
VTGGLVQQIT QWLSNMLGTT VDKLKPVFTW VEAKLSAGIE FLRDAWEILK
660 670 680 690 700
FLVTGVFDIV KGQIQVASDN LKECVKAFLD VLNKALEMCI DQVIIAGAKL
710 720 730 740 750
RTLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLRAPKEVT FFEGDSHDTV
760 770 780 790 800
FTSEEVVLKN GELEALETPV DSFTNGAVIG TPVCVNGLML LELKDKEQYC
810 820 830 840 850
ALSPGLLATN NVFSLKGGAP VKGVTFGEDT VLEVQGYKNV KITFELDERV
860 870 880 890 900
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE
910 920 930 940 950
WSVATFYLFD DAGEEKLSSR MYCSFYPPDE EEDCEEYEDE EEIPEETCEH
960 970 980 990 1000
EYGTEDDYKG LPLEFGASTE IQQVDEEEEE DWLEEAIAAK PEPEPLPEEP
1010 1020 1030 1040 1050
VNQFTGYLKL TDNVAIKCVD IVKEAQHAKP TVIVNAANVH LKHGGGVAGA
1060 1070 1080 1090 1100
LNKATNGAMQ QESDDYIKKN GPLTVGGSCL LSGHNLAKKC MHVVGPNLNA
1110 1120 1130 1140 1150
GEDVQLLKAA YANFNSQDVL LAPLLSAGIF GAKPLQSLKM CVETVRTQVY
1160 1170 1180 1190 1200
FAVNDQDLYD HVVLGYLDSL KPKVETPTQE NLELKEQPAV ETLTQENLEL
1210 1220 1230 1240 1250
EELPVIEKPV DVKFKARIEE VNTSLEETKF LTSRLLLFAD INGKLYQDSQ
1260 1270 1280 1290 1300
NMLRGEDMFF LEKDAPYIVG DVISSGDITC VIIPAKKAGG TTEMLAKALK
1310 1320 1330 1340 1350
KVPVSEYITT YPGQGCAGYT LEEAKTALRK CKSVFYVLPS KTPNDKEEIL
1360 1370 1380 1390 1400
GTVSWNLREM LAHAEETRKL MLICMDVKAL MSTIHRRYKG IKVQEGIVDY
1410 1420 1430 1440 1450
GVRFFFYTSK EPVASIITKL NLLNEPLVTM PIGYVTHGLN LEEAARCMRS
1460 1470 1480 1490 1500
LKAPAVVSVS SPDAVTTYNG YLTSSSKTSE EHFIETVSLA GMYRDWSYSG
1510 1520 1530 1540 1550
QRTELGVEFL KRGDKVVYHT VGSPIQFHLD GEVLLLDKLK SLLSLREVRT
1560 1570 1580 1590 1600
IKVFTTVDNT NLHTQIVDMS MTYGQQFGPT YLDGADVTKI KPHAKHEGKT
1610 1620 1630 1640 1650
FFVLPSDDTL RSEAFEYYHT LDESFLGRYM SALNHTKKWK FPQIGGLTSI
1660 1670 1680 1690 1700
KWADNNCYLS SVLLALQQIE VKFNAPALQE AYYRARAGDA ANFCALILAY
1710 1720 1730 1740 1750
SNRTVGELGD VRETMTHLLQ HANLESAKRV LNVVCKTCGQ KSTTLTGVEA
1760 1770 1780 1790 1800
VMYMGTLSYE ELKTGVTIPC ICGRDATQYL VQQESSFVMM SAPPSEYTLQ
1810 1820 1830 1840 1850
QGAFLCANEY TGSYQCGHYT HVTVKETLYR IDGAYLTKMS EYKGPVTDVF
1860 1870 1880 1890 1900
YKEISYTTTI KPVSYKLDGV IYTEIQPKLD EYYKKDNAYY TEQPIDLVPT
1910 1920 1930 1940 1950
QPLPNASFDN FKLTCSNTKF ADDLNQMTGF KKPASRELSV TFFPDLNGDV
1960 1970 1980 1990 2000
VAIDYRHYSA SFKKGAKLLH KPIIWHINQT TNKTTYKPNT WCLRCLWSTK
2010 2020 2030 2040 2050
PVETSNSFEV LEVEDTQGMD NLACESQTPT SEEVVENPTI QKEVIECDVK
2060 2070 2080 2090 2100
TIEVVGNVIL KPSEEGVKVT QELGHEDLMA AYVEETSITI KKPNELSLAL
2110 2120 2130 2140 2150
GLRTLATHGA AAINSVPWSK ILAYVKPFLG QAAVTTTNCI KRCVQRVFNN
2160 2170 2180 2190 2200
YMPYVITLLF QLCTFTRSTN SRIRASLPTT IAKNSVKSVA KLCLDVCINY
2210 2220 2230 2240 2250
VKSPKFSKLF TIAMWLLLLS ICLGSLIYVT AAFGVLLSNL GIPSYCDGVR
2260 2270 2280 2290 2300
ESYVNSSNVT TMDFCEGSFL CSVCLNGLDS LDSYPALETI QVTISSYKLD
2310 2320 2330 2340 2350
LTSLGLAAEW FLAYMLFTKF FYLLGLSAIM QVFFGYFASH FISNSWLMWF
2360 2370 2380 2390 2400
IISIVQMAPV SAMVRMYIFF AFCYYVWKSY VHIMDGCTSS TCMMCYKRNR
2410 2420 2430 2440 2450
ATRVECTTIV NGMKRSFYVY ANGGRGFCKA HNWNCLNCDT FCAGSTFISD
2460 2470 2480 2490 2500
EVARDLSLQF KRPINPTDQS SYVVDSVAVK NGALHLYFDK AGQKTYERHP
2510 2520 2530 2540 2550
LSHFVNLDNL RANNTKGSLP INVIVFDGKS KCDESAAKSA SVYYSQLMCQ
2560 2570 2580 2590 2600
PILLLDQALV SDVGDSTEVS VKMFDAYVDT FSATFSVPME KLKALVATAH
2610 2620 2630 2640 2650
SELAKGVALD GVLSTFVSAA RQGVVDTDVD TKDVIECLKL SHHSDLEVTG
2660 2670 2680 2690 2700
DSCNNFMLTY NKVENMTPRD LGACIDCNAR HINAQVAKSH NVSLIWNVKD
2710 2720 2730 2740 2750
YMSLSEQLRK QIRSAAKKNN IPFRLTCATT RQVVNAITTK ISLKGGKIVS
2760 2770 2780 2790 2800
TWFKLMLKAT LLCVLAALFC YIIMPVHSLS VHDGYTNEII GYKAIQDGVT
2810 2820 2830 2840 2850
RDIMATDDCF ANKHAGFDSW FSQRGGSYRN DKSCPVVAAI ITREIGFIVP
2860 2870 2880 2890 2900
GLPGTVLRAI NGDFLHFLPR VFSAVGNICY TPSKLIEYSD FATSACVLAA
2910 2920 2930 2940 2950
ECTIFKDAMG KPVPYCYDTN LLEGSISYSE LRPDTRYVLM DGSIIQFPNT
2960 2970 2980 2990 3000
YLEGSVRVVT TFDAEYCRHG TCERSEAGVC LSTSGRWVLN NEHYRALPGV
3010 3020 3030 3040 3050
FCGVDAMNLI ANIFTPLVQP VGALDVSASV VAGGIIAILV TCAAYYFMKF
3060 3070 3080 3090 3100
RRAFGEYNHV VAANALLFLM SFTILCLAPA YSFLPGVYSI FYLYLTFYFT
3110 3120 3130 3140 3150
NDVSFLAHLQ WFAMFSPIVP FWITAIYVFC ISLKHCHWFF NNYLRKRVMF
3160 3170 3180 3190 3200
NGVTFSTFEE AALCTFLLNK EMYLKLRSET LLPLTQYNRY LALYNKYKYF
3210 3220 3230 3240 3250
SGALDTTSYR EAACCHLAKA LNDFSNSGAD VLYQPPQTSI TSAVLQSGFR
3260 3270 3280 3290 3300
KMAFPSGKVE GCMVQVTCGT TTLNGLWLDD TVYCPRHVIC TAEDMLNPNY
3310 3320 3330 3340 3350
EDLLIRKSNH SFLVQAGNVQ LRVIGHSMQN CLLRLKVDTS NPKTPKYKFV
3360 3370 3380 3390 3400
RIQPGQTFSV LACYNGSPSG VYQCAMRPNY TIKGSFLNGS CGSVGFNIDY
3410 3420 3430 3440 3450
DCVSFCYMHH MELPTGVHAG TDLEGKFYGP FVDRQTAQAA GTDTTITLNV
3460 3470 3480 3490 3500
LAWLYAAVIN GDRWFLNRFT TTLNDFNLVA MKYNYEPLTQ DHVDILGPLS
3510 3520 3530 3540 3550
AQTGIAVLDM CAALKELLQN GMNGRTILGS TILEDEFTPF DVVRQCSGVT
3560 3570 3580 3590 3600
FQGKFKKIVK GTHHWMLLTF LTSLLILVQS TQWSLFFFVY ENAFLPFTLG
3610 3620 3630 3640 3650
IMAIAACAML LVKHKHAFLC LFLLPSLATV AYFNMVYMPA SWVMRIMTWL
3660 3670 3680 3690 3700
ELADTSLSGY RLKDCVMYAS ALVLLVLMTA RTVYDDAARR VWTLMNVITL
3710 3720 3730 3740 3750
VYKVYYGNSL DQAISMWALV ISVTSNYSGV VTTIMFLARA IVFVCVEYYP
3760 3770 3780 3790 3800
LLFITGNTLQ CIMLVYCFLG YCCCCYFGLF CLLNRYFRLT LGVYDYLVST
3810 3820 3830 3840 3850
QEFRYMNSQG LLPPKSSIDA FKLNIKLLGI GGKPCIKVAT VQSKMSDVKC
3860 3870 3880 3890 3900
TSVVLLSVLQ QLRVESSSKL WAQCVQLHND ILLAKDTTEA FEKMVSLLSV
3910 3920 3930 3940 3950
LLSMQGAVDI NKLCEEMLDN RATLQAIASE FSSLPSYAAY ATAQEAYEQA
3960 3970 3980 3990 4000
VANGDSEVVL KKLKKSLNVA KSEFDRDAAM QRKLEKMADQ AMTQMYKQAR
4010 4020 4030 4040 4050
SEDKRAKVTS AMQTMLFTML RKLDNDALNN IINNARDGCV PLNIIPLTTA
4060 4070 4080 4090 4100
AKLMVVVPDY GTYKNTCDGN TFTYASALWE IQQVVDADSK IVQLSEINMD
4110 4120 4130 4140 4150
NSQNLAWPLI VTALRANSAV KLQNNELSPV ALRQMSCAAG TTQTACTDDN
4160 4170 4180 4190 4200
ALAYYNNSKG GRFVLALLSD HQDLKWARFP KSDGTGTIYT ELEPPCRFVT
4210 4220 4230 4240 4250
DTPRGPKVKY LYFIKGLNNL NRGMVLGSLA ATVRLQAGNA TEVPANSAVL
4260 4270 4280 4290 4300
SFCAFAVDPA KAYKDYLASG GQPITNCVKM LCTHTGTGQA ITVTPEANMD
4310 4320 4330 4340 4350
QESFGGASCC LYCRCHIDHP NPKGFCDLKG KYVQIPATCA NDPVGFTLKN
4360 4370 4380 4390 4400
TVCTVCGTWK GYGCSCDQLR EPMMQSADAS TFLNRVCGVS AARLTPCGTG
4410 4420 4430 4440 4450
TSTDVVYRAF DIYNERVAGF AKFLKTNCCR FQEKDEEGNL LDSYFVVKRH
4460 4470 4480 4490 4500
TMSNYQHEET IYNLVKECPA VAVHDFFKFR VDGDMVPHIS RQRLTKYTMA
4510 4520 4530 4540 4550
DLVYALRHFD EGNCDTLKEI LVTYNCCDDD YFNKKDWYDF VENPDILRVY
4560 4570 4580 4590 4600
ANLGERVRQA LLKTVQFCDA MRDAGIVGVL TLDNQDLNGN WYDFGDFVQV
4610 4620 4630 4640 4650
APGCGVPIVD SYYSLLMPIL TLTKALAAES HMDADLAKPL IKWDLLKYDF
4660 4670 4680 4690 4700
TEERLCLFDR YFKYWDQTYH PNCINCLDDR CILHCANFNV LFSTVFPPTS
4710 4720 4730 4740 4750
FGPLVRKIFV DGVPFVVSTG YHFRELGVVH NQDVNLHSSR LSFKELLVYA
4760 4770 4780 4790 4800
ADPAMHAASG NLLLDKRTTC FSVAALTNNV AFQTVKPGNF NKDFYDFAVS
4810 4820 4830 4840 4850
KGFFKEGSSV ELKHFFFAQD GNAAISDYDY YRYNLPTMCD IRQLLFVVEV
4860 4870 4880 4890 4900
VDKYFDCYDG GCINANQVIV NNLDKSAGFP FNKWGKARLY YDSMSYEDQD
4910 4920 4930 4940 4950
VLFAYTKRNV IPTITQMNLK YAISAKNRAR TVAGVSICST MTNRQFHQKL
4960 4970 4980 4990 5000
LKSIAATRGA TVVIGTSKFY GGWHNMLKTV YSDVETPHLM GWDYPKCDRA
5010 5020 5030 5040 5050
MPNMLRIMAS LVLARKHSTC CNLSHRFYRL ANECAQVLSE MVMCGGSLYV
5060 5070 5080 5090 5100
KPGGTSSGDA TTAYANSVFN ICQAVTANVN ALLSTDGNKI ADKYVRNLQH
5110 5120 5130 5140 5150
RLYECLYRNR DVDHEFVDEF YAYLRKHFSM MILSDDAVVC YNSNYAAQGL
5160 5170 5180 5190 5200
VASIKNFKAV HYYQNNVFMS EAKCWTETDL TKGPHEFCSQ HTMLVKQGDD
5210 5220 5230 5240 5250
YVYLPYPDPS RILGAGCFVD DIVKTDGTLM IERFVSLAID AYPLTKHPNQ
5260 5270 5280 5290 5300
EYADVFHLYL QYIRKLHDEL TGHMLDMYSV MLTNDNTSRY WEPEFYEAMY
5310 5320 5330 5340 5350
TPHTVLQAVG ACVLCNSQTS LRCGACIRRP FLCCKCCYDH VISTSHKLVL
5360 5370 5380 5390 5400
SVNPYVCNAP GCDVTDVTQL YLGGMSYYCK LHKPPISFPL CANGQVFGLY
5410 5420 5430 5440 5450
KNTCVGSDNV TDFNAIATCD WTNAGDYILA NTCTERLKLF AAETLKATEE
5460 5470 5480 5490 5500
TFKLSYGIAT VREVLSDREL HLSWEVGKPR PPLNRNYVFT GYRVTKNSKV
5510 5520 5530 5540 5550
QIGEYTFEKG DYGDAVVYRG TTTYKLNVGD YFVLTSHTVM PLSAPTLVPQ
5560 5570 5580 5590 5600
EHYVRITGLY PTLNISDEFS SNVANYQKVG MQKYSTLQGP PGTGKSHFAI
5610 5620 5630 5640 5650
GLALYYPSAR IVYTACSHAA VDALCEKALK YLPIDKCSRI IPARARVECF
5660 5670 5680 5690 5700
DKFKVNSTLE QYVFCTVNAL PETTADIVVF DEISMATNYD LSVVNARLRA
5710 5720 5730 5740 5750
KHYVYIGDPA QLPAPRTLLT KGTLEPEYFN SVCRLMKTIG PDMFLGTCRR
5760 5770 5780 5790 5800
CPAEIVDTVS ALVYDNKLKA HKEKSAQCFK MFYKGVITHD VSSAINRPQI
5810 5820 5830 5840 5850
GVVREFLTRN PAWRKAVFIS PYNSQNAVAS KILGLPTQTV DSSQGSEYDY
5860 5870 5880 5890 5900
VIFTQTTETA HSCNVNRFNV AITRAKIGIL CIMSDRDLYD KLQFTSLEVP
5910 5920 5930 5940 5950
RRNVATLQAE NVTGLFKDCS KIITGLHPTQ APTHLSVDTK FKTEGLCVDI
5960 5970 5980 5990 6000
PGIPKDMTYR RLISMMGFKM NYQVNGYPNM FITREEAIRH VRAWIGFDVE
6010 6020 6030 6040 6050
GCHATRDAVG TNLPLQLGFS TGVNLVAVPT GYVDTENNTE FTRVNAKPPP
6060 6070 6080 6090 6100
GDQFKHLIPL MYKGLPWNVV RIKIVQMLSD TLKGLSDRVV FVLWAHGFEL
6110 6120 6130 6140 6150
TSMKYFVKIG PERTCCLCDR RATCFSTSSD TYACWNHSVG FDYVYNPFMI
6160 6170 6180 6190 6200
DVQQWGFTGN LQSNHDQHCQ VHGNAHVASC DAIMTRCLAV HECFVKRVDW
6210 6220 6230 6240 6250
SVEYPIIGDE LKINAACRKV QHMVVKSALL ADKFSVLHDI GNPKAIKCVP
6260 6270 6280 6290 6300
QAEVDWKFYD AQPCSDKAYK IEELFYSYAT HHDKFTDGVC LFWNCNVDRY
6310 6320 6330 6340 6350
PANAIVCRFD TRVLSNLNLP GCDGGSLYVN KHAFHTPAFD KSAFTYLKQL
6360 6370 6380 6390 6400
PFFYYSDSPC ESHGKQVVSD IDYVPLKSAT CITRCNLGGA VCRRHANEYR
6410 6420 6430 6440 6450
QYLDAYNMMI SAGFSLWIYK QFDTYNLWNT FTRLQSLENV AYNVVNKGHF
6460 6470 6480 6490 6500
DGQIGEAPVS IINNAVYTKV DGNDVEIFEN KTTLPVNVAF ELWAKRNIKP
6510 6520 6530 6540 6550
VPEIKILNNL GVDIAANTVI WDYKREAPAH VSTIGVCTMT DIAKKPTESA
6560 6570 6580 6590 6600
CSSLTVLFDG RVEGQVDLFR NARNGVLITE GSVKGLTPSK GPAQASVNGV
6610 6620 6630 6640 6650
TLIGESVKTQ FNYFKKVDGI IQQLPETYFT QSRDLEDFKP RSKMETDFLE
6660 6670 6680 6690 6700
LAMDEFIQRY KLEGYAFEHI VYGDFSHGQL GGLHLMIGLA KRSQDSPLKL
6710 6720 6730 6740 6750
EDFIPTDSTV KNYFITDAQT GSSKCVCSVI DLLLDDFVEI IKSQDLSVIS
6760 6770 6780 6790 6800
KVVKVTIDYA EISFMLWCKD GHVETFYPKL QASQAWQPGV AMPNLYKMQR
6810 6820 6830 6840 6850
MLLEKCDLQN YGENAVIPKG IMMNVAKYTQ LCQYLNTLTL AVPYNMRVIH
6860 6870 6880 6890 6900
FGAGSDKGVA PGTAVLRQWL PTGALLVDSD LNDFVSDADS TLIGDCATVH
6910 6920 6930 6940 6950
TANKWDLIIS DMYDPKTKHV TKENDSKEGF FTYLCGFIKQ KLALGGSVAV
6960 6970 6980 6990 7000
KITEHSWNAD LYKLMGHFSW WTAFVTNVNA SSSEAFLIGV NYLGKLREQI
7010 7020 7030 7040 7050
DGYTMHANYI FWRNTNPIQL SSYSLFDMSK FPLKLRGTAV MSLKENQIND
7060 7070
MIYSLLENGR LIIRENNRVV VSSDILVNN

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,079
Mass (Da):791,654
Last modified:June 10, 2008 - v1
Checksum:iCE92AE7AE019B902
GO
Isoform Replicase polyprotein 1a (identifier: P0C6F5-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

The sequence of this isoform can be found in the external entry P0C6F5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Length:4,388
Mass (Da):487,709
GO

Sequence cautioni

The sequence ABG47068.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ648857 Genomic RNA. Translation: ABG47068.1. Different initiation.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ648857 Genomic RNA. Translation: ABG47068.1 . Different initiation.

3D structure databases

ProteinModelPortali P0C6V9.
SMRi P0C6V9. Positions 13-127, 819-930, 1000-1174, 1337-1475, 1547-1860, 3247-3552, 3842-3925, 3927-4117, 4124-4236, 4246-4368, 6436-6779.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR021590. NSP1.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF06478. Corona_RPol_N. 1 hit.
PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1AB_BC279
AccessioniPrimary (citable) accession number: P0C6V9
Secondary accession number(s): Q0Q476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus 279/2005 is highly similar to SARS-CoV (SARS-like).

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3