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P0C6V2 (R1A_CVPPU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1a

Short name=pp1a
Alternative name(s):
ORF1a polyprotein

Cleaved into the following 11 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p9
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p87
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.-
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PLP1/PLP2
    Papain-like proteinases 1/2
    p195
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p34
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p5
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p23
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p14
  11. Non-structural protein 11
    Short name=nsp11
Gene names
ORF Names:1a
OrganismPorcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV) [Complete proteome]
Taxonomic identifier11151 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length4017 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO is autocatalytically processed.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandMetal-binding
RNA-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host IRF3 activity

Inferred from electronic annotation. Source: UniProtKB-KW

viral genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: InterPro

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1a (identifier: P0C6V2-1)

Also known as: pp1a; ORF1a polyprotein;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6Y5-1)

Also known as: pp1ab;

The sequence of this isoform can be found in the external entry P0C6Y5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 40174017Replicase polyprotein 1a
PRO_0000338302
Chain1 – 110110Non-structural protein 1 By similarity
PRO_0000338303
Chain111 – 879769Non-structural protein 2 By similarity
PRO_0000338304
Chain880 – 23881509Non-structural protein 3 By similarity
PRO_0000338305
Chain2389 – 2878490Non-structural protein 4 By similarity
PRO_0000338306
Chain2879 – 31803023C-like proteinase
PRO_0000338307
Chain3181 – 3474294Non-structural protein 6 By similarity
PRO_0000338308
Chain3475 – 355783Non-structural protein 7 By similarity
PRO_0000338309
Chain3558 – 3752195Non-structural protein 8 By similarity
PRO_0000338310
Chain3753 – 3863111Non-structural protein 9
PRO_0000338311
Chain3864 – 3998135Non-structural protein 10 By similarity
PRO_0000338312
Chain3999 – 401719Non-structural protein 11 Potential
PRO_0000338313

Regions

Transmembrane1896 – 191621Helical; Potential
Transmembrane1995 – 201521Helical; Potential
Transmembrane2033 – 205321Helical; Potential
Transmembrane2401 – 242121Helical; Potential
Transmembrane2467 – 248721Helical; Potential
Transmembrane2497 – 251721Helical; Potential
Transmembrane2538 – 255821Helical; Potential
Transmembrane2666 – 268621Helical; Potential
Transmembrane2695 – 271521Helical; Potential
Transmembrane2721 – 274121Helical; Potential
Transmembrane2746 – 276621Helical; Potential
Transmembrane3187 – 320721Helical; Potential
Transmembrane3217 – 323721Helical; Potential
Transmembrane3242 – 326221Helical; Potential
Transmembrane3280 – 330021Helical; Potential
Transmembrane3313 – 333321Helical; Potential
Transmembrane3347 – 336721Helical; Potential
Transmembrane3371 – 339121Helical; Potential
Transmembrane3394 – 341421Helical; Potential
Domain1055 – 1299245Peptidase C16 1
Domain1318 – 1489172Macro
Domain1550 – 1803254Peptidase C16 2
Domain2879 – 3180302Peptidase C30
Zinc finger1164 – 119532C4-type
Zinc finger3937 – 395317 By similarity
Zinc finger3979 – 399214 By similarity
Region1896 – 2053158HD1
Region2401 – 2766366HD2
Region3187 – 3414228HD3
Compositional bias932 – 1042111Glu-rich

Sites

Active site10931For PL1-PRO activity By similarity
Active site12441For PL1-PRO activity By similarity
Active site15881For PL2-PRO activity By similarity
Active site17411For PL2-PRO activity By similarity
Active site29191For 3CL-PRO activity By similarity
Active site30221For 3CL-PRO activity By similarity
Site110 – 1112Cleavage; by PL1-PRO By similarity
Site879 – 8802Cleavage; by PL1-PRO By similarity
Site2388 – 23892Cleavage; by PL2-PRO By similarity
Site2878 – 28792Cleavage; by 3CL-PRO By similarity
Site3180 – 31812Cleavage; by 3CL-PRO By similarity
Site3474 – 34752Cleavage; by 3CL-PRO By similarity
Site3557 – 35582Cleavage; by 3CL-PRO By similarity
Site3752 – 37532Cleavage; by 3CL-PRO By similarity
Site3863 – 38642Cleavage; by 3CL-PRO By similarity
Site3998 – 39992Cleavage; by 3CL-PRO By similarity

Natural variations

Natural variant5721F → S in strain: Isolate Purdue-115.
Natural variant10411E → D in strain: Isolate Purdue-115.
Natural variant23751P → T in strain: Isolate Purdue-115.
Natural variant23811E → Q in strain: Isolate Purdue-115.

Secondary structure

.................................................................................................... 4017
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 97ED2B170C4FD248

FASTA4,017447,352
        10         20         30         40         50         60 
MSSKQFKILV NEDYQVNVPS LPIRDVLQEI KYCYRNGFEG YVFVPEYCRD LVDCDRKDHY 

        70         80         90        100        110        120 
VIGVLGNGVS DLKPVLLTEP SVMLQGFIVR ANCNGVLEDF DLKIARTGRG AIYVDQYMCG 

       130        140        150        160        170        180 
ADGKPVIEGD FKDYFGDEDI IEFEGEEYHC AWTTVRDEKP LNQQTLFTIQ EIQYNLDIPH 

       190        200        210        220        230        240 
KLPNCATRHV APPVKKNSKI VLSEDYKKLY DIFGSPFMGN GDCLSKCFDT LHFIAATLRC 

       250        260        270        280        290        300 
PCGSESSGVG DWTGFKTACC GLSGKVKGVT LGDIKPGDAV VTSMSAGKGV KFFANCVLQY 

       310        320        330        340        350        360 
AGDVEGVSIW KVIKTFTVDE TVCTPGFEGE LNDFIKPESK SLVACSVKRA FITGDIDDAV 

       370        380        390        400        410        420 
HDCIITGKLD LSTNLFGNVG LLFKKTPWFV QKCGALFVDA WKVVEELCGS LTLTYKQIYE 

       430        440        450        460        470        480 
VVASLCTSAF TIVNYKPTFV VPDNRVKDLV DKCVKVLVKA FDVFTQIITI AGIEAKCFVL 

       490        500        510        520        530        540 
GAKYLLFNNA LVKLVSVKIL GKKQKGLECA FFATSLVGAT VNVTPKRTET ATISLNKVDD 

       550        560        570        580        590        600 
VVAPGEGYIV IVGDMAFYKS GEYYFMMSSP NFVLTNNVFK AVKVPSYDIV YDVDNDTKSK 

       610        620        630        640        650        660 
MIAKLGSSFE YDGDIDAAIV KVNELLIEFR QQSLCFRAFK DDKSIFVEAY FKKYKMPACL 

       670        680        690        700        710        720 
AKHIGLWNII KKDSCKRGFL NLFNHLNELE DIKETNIQAI KNILCPDPLL DLDYGAIWYN 

       730        740        750        760        770        780 
CMPGCSDPSV LGSVQLLIGN GVKVVCDGCK GFANQLSKGY NKLCNAARND IEIGGIPFST 

       790        800        810        820        830        840 
FKTPTNTFIE MTDAIYSVIE QGKALSFRDA DVPVVDNGTI STADWSEPIL LEPAEYVKPK 

       850        860        870        880        890        900 
NNGNVIVIAG YTFYKDEDEH FYPYGFGKIV QRMYNKMGGG DKTVSFSEEV DVQEIAPVTR 

       910        920        930        940        950        960 
VKLEFEFDNE IVTGVLERAI GTRYKFTGTT WEEFEESISE ELDAIFDTLA NQGVELEGYF 

       970        980        990       1000       1010       1020 
IYDTCGGFDI KNPDGIMISQ YDINITADEK SEVSASSEEE EVESVEEDPE NEIVEASEGA 

      1030       1040       1050       1060       1070       1080 
EGTSSQEEVE TVEVADITST EEDVDIVEVS AKDDPWAAAV DVQEAEQFNP SLPPFKTTNL 

      1090       1100       1110       1120       1130       1140 
NGKIILKQGD NNCWINACCY QLQAFDFFNN EAWEKFKKGD VMDFVNLCYA ATTLARGHSG 

      1150       1160       1170       1180       1190       1200 
DAEYLLELML NDYSTAKIVL AAKCGCGEKE IVLERAVFKL TPLKESFNYG VCGDCMQVNT 

      1210       1220       1230       1240       1250       1260 
CRFLSVEGSG VFVHDILSKQ TPEAMFVVKP VMHAVYTGTT QNGHYMVDDI EHGYCVDGMG 

      1270       1280       1290       1300       1310       1320 
IKPLKKRCYT STLFINANVM TRAEKPKQEF KVEKVEQQPI VEENKSSIEK EEIQSPKNDD 

      1330       1340       1350       1360       1370       1380 
LILPFYKAGK LSFYQGALDV LINFLEPDVI VNAANGDLKH MGGVARAIDV FTGGKLTERS 

      1390       1400       1410       1420       1430       1440 
KDYLKKNKSI APGNAVFFEN VIEHLSVLNA VGPRNGDSRV EAKLCNVYKA IAKCEGKILT 

      1450       1460       1470       1480       1490       1500 
PLISVGIFNV RLETSLQCLL KTVNDRGLNV FVYTDQERQT IENFFSCSIP VNVTEDNVNH 

      1510       1520       1530       1540       1550       1560 
ERVSVSFDKT YGEQLKGTVV IKDKDVTNQL PSAFDVGQKV IKAIDIDWQA HYGFRDAAAF 

      1570       1580       1590       1600       1610       1620 
SASSHDAYKF EVVTHSNFIV HKQTDNNCWI NAICLALQRL KPQWKFPGVR GLWNEFLERK 

      1630       1640       1650       1660       1670       1680 
TQGFVHMLYH ISGVKKGEPG DAELMLHKLG DLMDNDCEII VTHTTACDKC AKVEKFVGPV 

      1690       1700       1710       1720       1730       1740 
VAAPLAIHGT DETCVHGVSV NVKVTQIKGT VAITSLIGPI IGEVLEATGY ICYSGSNRNG 

      1750       1760       1770       1780       1790       1800 
HYTYYDNRNG LVVDAEKAYH FNRDLLQVTT AIASNFVVKK PQAEERPKNC AFNKVAASPK 

      1810       1820       1830       1840       1850       1860 
IVQEQKLLAI ESGANYALTE FGRYADMFFM AGDKILRLLL EVFKYLLVLF MCLRSTKMPK 

      1870       1880       1890       1900       1910       1920 
VKVKPPLAFK DFGAKVRTLN YMRQLNKPSV WRYAKLVLLL IAIYNFFYLF VSIPVVHKLT 

      1930       1940       1950       1960       1970       1980 
CNGAVQAYKN SSFIKSAVCG NSILCKACLA SYDELADFQH LQVTWDFKSD PLWNRLVQLS 

      1990       2000       2010       2020       2030       2040 
YFAFLAVFGN NYVRCFLMYF VSQYLNLWLS YFGYVEYSWF LHVVNFESIS AEFVIVVIVV 

      2050       2060       2070       2080       2090       2100 
KAVLALKHIV FACSNPSCKT CSRTARQTRI PIQVVVNGSM KTVYVHANGT GKFCKKHNFY 

      2110       2120       2130       2140       2150       2160 
CKNCDSYGFE NTFICDEIVR DLSNSVKQTV YATDRSHQEV TKVECSDGFY RFYVGDEFTS 

      2170       2180       2190       2200       2210       2220 
YDYDVKHKKY SSQEVLKSML LLDDFIVYSP SGSALANVRN ACVYFSQLIG KPIKIVNSDL 

      2230       2240       2250       2260       2270       2280 
LEDLSVDFKG ALFNAKKNVI KNSFNVDVSE CKNLDECYRA CNLNVSFSTF EMAVNNAHRF 

      2290       2300       2310       2320       2330       2340 
GILITDRSFN NFWPSKVKPG SSGVSAMDIG KCMTSDAKIV NAKVLTQRGK SVVWLSQDFA 

      2350       2360       2370       2380       2390       2400 
ALSSTAQKVL VKTFVEEGVN FSLTFNAVGS DDDLPYERFT ESVSPKSGSG FFDVITQLKQ 

      2410       2420       2430       2440       2450       2460 
IVILVFVFIF ICGLCSVYSV ATQSYIESAE GYDYMVIKNG IVQPFDDTIS CVHNTYKGFG 

      2470       2480       2490       2500       2510       2520 
DWFKAKYGFI PTFGKSCPIV VGTVFDLENM RPIPDVPAYV SIVGRSLVFA INAAFGVTNM 

      2530       2540       2550       2560       2570       2580 
CYDHTGNAVS KDSYFDTCVF NTACTTLTGL GGTIVYCAKQ GLVEGAKLYS DLMPDYYYEH 

      2590       2600       2610       2620       2630       2640 
ASGNMVKLPA IIRGLGLRFV KTQATTYCRV GECIDSKAGF CFGGDNWFVY DNEFGNGYIC 

      2650       2660       2670       2680       2690       2700 
GNSVLGFFKN VFKLFNSNMS VVATSGAMLV NIIIACLAIA MCYGVLKFKK IFGDCTFLIV 

      2710       2720       2730       2740       2750       2760 
MIIVTLVVNN VSYFVTQNTF FMIIYAIVYY FITRKLAYPG ILDAGFIIAY INMAPWYVIT 

      2770       2780       2790       2800       2810       2820 
AYILVFLYDS LPSLFKLKVS TNLFEGDKFV GNFESAAMGT FVIDMRSYET IVNSTSIARI 

      2830       2840       2850       2860       2870       2880 
KSYANSFNKY KYYTGSMGEA DYRMACYAHL GKALMDYSVN RTDMLYTPPT VSVNSTLQSG 

      2890       2900       2910       2920       2930       2940 
LRKMAQPSGL VEPCIVRVSY GNNVLNGLWL GDEVICPRHV IASDTTRVIN YENEMSSVRL 

      2950       2960       2970       2980       2990       3000 
HNFSVSKNNV FLGVVSARYK GVNLVLKVNQ VNPNTPEHKF KSIKAGESFN ILACYEGCPG 

      3010       3020       3030       3040       3050       3060 
SVYGVNMRSQ GTIKGSFIAG TCGSVGYVLE NGILYFVYMH HLELGNGSHV GSNFEGEMYG 

      3070       3080       3090       3100       3110       3120 
GYEDQPSMQL EGTNVMSSDN VVAFLYAALI NGERWFVTNT SMSLESYNTW AKTNSFTELS 

      3130       3140       3150       3160       3170       3180 
STDAFSMLAA KTGQSVEKLL DSIVRLNKGF GGRTILSYGS LCDEFTPTEV IRQMYGVNLQ 

      3190       3200       3210       3220       3230       3240 
AGKVKSFFYP IMTAMTILFA FWLEFFMYTP FTWINPTFVS IVLAVTTLIS TVFVSGIKHK 

      3250       3260       3270       3280       3290       3300 
MLFFMSFVLP SVILVTAHNL FWDFSYYESL QSIVENTNTM FLPVDMQGVM LTVFCFIVFV 

      3310       3320       3330       3340       3350       3360 
TYSVRFFTCK QSWFSLAVTT ILVIFNMVKI FGTSDEPWTE NQIAFCFVNM LTMIVSLTTK 

      3370       3380       3390       3400       3410       3420 
DWMVVIASYR IAYYIVVCVM PSAFVSDFGF MKCISIVYMA CGYLFCCYYG ILYWVNRFTC 

      3430       3440       3450       3460       3470       3480 
MTCGVYQFTV SAAELKYMTA NNLSAPKNAY DAMILSAKLI GVGGKRNIKI STVQSKLTEM 

      3490       3500       3510       3520       3530       3540 
KCTNVVLLGL LSKMHVESNS KEWNYCVGLH NEINLCDDPE IVLEKLLALI AFFLSKHNTC 

      3550       3560       3570       3580       3590       3600 
DLSELIESYF ENTTILQSVA SAYAALPSWI ALEKARADLE EAKKNDVSPQ ILKQLTKAFN 

      3610       3620       3630       3640       3650       3660 
IAKSDFEREA SVQKKLDKMA EQAAASMYKE ARAVDRKSKI VSAMHSLLFG MLKKLDMSSV 

      3670       3680       3690       3700       3710       3720 
NTIIDQARNG VLPLSIIPAA SATRLVVITP SLEVFSKIRQ ENNVHYAGAI WTIVEVKDAN 

      3730       3740       3750       3760       3770       3780 
GSHVHLKEVT AANELNLTWP LSITCERTTK LQNNEIMPGK LKERAVRASA TLDGEAFGSG 

      3790       3800       3810       3820       3830       3840 
KALMASESGK SFMYAFIASD NNLKYVKWES NNDIIPIELE APLRFYVDGA NGPEVKYLYF 

      3850       3860       3870       3880       3890       3900 
VKNLNTLRRG AVLGYIGATV RLQAGKPTEH PSNSSLLTLC AFSPDPAKAY VDAVKRGMQP 

      3910       3920       3930       3940       3950       3960 
VNNCVKMLSN GAGNGMAVTN GVEANTQQDS YGGASVCIYC RCHVEHPAID GLCRYKGKFV 

      3970       3980       3990       4000       4010 
QIPTGTQDPI RFCIENEVCV VCGCWLNNGC MCDRTSMQSF TVDQSYLNEC GVLVQLD 

« Hide

Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

See P0C6Y5.

References

[1]"Complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus."
Eleouet J., Rasschaert D., Lambert P., Levy L., Vende P., Laude H.
Virology 206:817-822(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Isolate Purdue-115.
[2]"Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome."
Almazan F., Gonzalez J.M., Penzes Z., Izeta A., Calvo E., Plana-Duran J., Enjuanes L.
Proc. Natl. Acad. Sci. U.S.A. 97:5516-5521(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate PUR46-MAD.
[3]"Conservation of substrate specificities among coronavirus main proteases."
Hegyi A., Ziebuhr J.
J. Gen. Virol. 83:595-599(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.
Strain: Isolate Purdue-115.
[4]"Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain."
Anand K., Palm G.J., Mesters J.R., Siddell S.G., Ziebuhr J., Hilgenfeld R.
EMBO J. 21:3213-3224(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 2879-3180.
[5]"Coronavirus main proteinase (3CLpro) structure: basis for design of anti-SARS drugs."
Anand K., Ziebuhr J., Wadhwani P., Mesters J.R., Hilgenfeld R.
Science 300:1763-1767(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.37 ANGSTROMS) OF 2879-3180 COMPLEXED WITH THE SUBSTRATE-ANALOG HEXAPEPTIDYL CMK.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z34093 mRNA. Translation: CAA83979.1.
AJ271965 Genomic RNA. Translation: CAB91144.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LVOX-ray1.96A/B/C/D/E/F2879-3180[»]
1P9UX-ray2.37A/B/C/D/E/F2879-3180[»]
2AMPX-ray2.70A/B2879-3180[»]
3MP2X-ray2.50A1071-1281[»]
3ZBDX-ray1.49A/B1-105[»]
4F49X-ray2.25A/B/C/D2879-3181[»]
ProteinModelPortalP0C6V2.
SMRP0C6V2. Positions 2879-3179, 3869-3994.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF01661. Macro. 1 hit.
PF09401. NSP10. 2 hits.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 2 hits.
SSF50494. SSF50494. 1 hit.
PROSITEPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0C6V2.

Entry information

Entry nameR1A_CVPPU
AccessionPrimary (citable) accession number: P0C6V2
Secondary accession number(s): Q9IW06
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references