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P0C6V1

- R1A_CVMJH

UniProt

P0C6V1 - R1A_CVMJH

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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei247 – 2482Cleavage; by PL1-PROCurated
Sitei832 – 8332Cleavage; by PL1-PROCurated
Active sitei1120 – 11201For PL1-PRO activityPROSITE-ProRule annotation
Active sitei1271 – 12711For PL1-PRO activityPROSITE-ProRule annotation
Active sitei1715 – 17151For PL2-PRO activityPROSITE-ProRule annotation
Active sitei1872 – 18721For PL2-PRO activityPROSITE-ProRule annotation
Sitei2840 – 28412Cleavage; by PL2-PROCurated
Sitei3336 – 33372Cleavage; by 3CL-PROCurated
Active sitei3377 – 33771For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3481 – 34811For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3639 – 36402Cleavage; by 3CL-PROCurated
Sitei3927 – 39282Cleavage; by 3CL-PROCurated
Sitei4019 – 40202Cleavage; by 3CL-PROCurated
Sitei4213 – 42142Cleavage; by 3CL-PROCurated
Sitei4323 – 43242Cleavage; by 3CL-PROCurated
Sitei4460 – 44612Cleavage; by 3CL-PROCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1197 – 122529C4-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1793 – 182937C4-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4397 – 441317By similarityAdd
BLAST
Zinc fingeri4439 – 445214By similarityAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA-directed RNA polymerase activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. viral genome replication Source: InterPro
  8. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p28
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65
Alternative name(s):
PL1-PRO/PL2-PRO
PL1/PL2
Papain-like proteinases 1/2
p210
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
p44
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p27
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p10
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p22
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p15
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiMurine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Taxonomic identifieri11144 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007193: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2835 – 28351F → A: No processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2836 – 28361S → A: No effect. 1 Publication
Mutagenesisi2837 – 28371L → A: No effect. 1 Publication
Mutagenesisi2838 – 28381K → A: No effect. 1 Publication
Mutagenesisi2838 – 28381K → N: No effect. 1 Publication
Mutagenesisi2839 – 28391G → A: Partial processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2839 – 28391G → N: No processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2839 – 28391G → V: No processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2840 – 28401G → A: No processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2840 – 28401G → N: No processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2840 – 28401G → V: No processing between p210 and peptide HD2. 1 Publication
Mutagenesisi2841 – 28411A → N: No effect. 1 Publication
Mutagenesisi2842 – 28421V → N or M: No effect. 1 Publication
Mutagenesisi2846 – 28461V → M: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 44744474Replicase polyprotein 1aPRO_0000338290Add
BLAST
Chaini1 – 247247Non-structural protein 1CuratedPRO_0000338291Add
BLAST
Chaini248 – 832585Non-structural protein 2CuratedPRO_0000338292Add
BLAST
Chaini833 – 28402008Non-structural protein 3CuratedPRO_0000338293Add
BLAST
Chaini2841 – 3336496Non-structural protein 4CuratedPRO_0000338294Add
BLAST
Chaini3337 – 36393033C-like proteinaseCuratedPRO_0000338295Add
BLAST
Chaini3640 – 3927288Non-structural protein 6CuratedPRO_0000338296Add
BLAST
Chaini3928 – 401992Non-structural protein 7CuratedPRO_0000338297Add
BLAST
Chaini4020 – 4213194Non-structural protein 8PRO_0000338298Add
BLAST
Chaini4214 – 4323110Non-structural protein 9CuratedPRO_0000338299Add
BLAST
Chaini4323 – 4474152Non-structural protein 11Sequence AnalysisPRO_0000338301Add
BLAST
Chaini4324 – 4460137Non-structural protein 10CuratedPRO_0000338300Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6V1.
SMRiP0C6V1. Positions 4055-4205, 4329-4453.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2289 – 230921HelicalSequence AnalysisAdd
BLAST
Transmembranei2320 – 234021HelicalSequence AnalysisAdd
BLAST
Transmembranei2403 – 242321HelicalSequence AnalysisAdd
BLAST
Transmembranei2445 – 246521HelicalSequence AnalysisAdd
BLAST
Transmembranei2846 – 286621HelicalSequence AnalysisAdd
BLAST
Transmembranei3099 – 311921HelicalSequence AnalysisAdd
BLAST
Transmembranei3121 – 314121HelicalSequence AnalysisAdd
BLAST
Transmembranei3153 – 317321HelicalSequence AnalysisAdd
BLAST
Transmembranei3180 – 320021HelicalSequence AnalysisAdd
BLAST
Transmembranei3205 – 322521HelicalSequence AnalysisAdd
BLAST
Transmembranei3648 – 366821HelicalSequence AnalysisAdd
BLAST
Transmembranei3678 – 369821HelicalSequence AnalysisAdd
BLAST
Transmembranei3705 – 372521HelicalSequence AnalysisAdd
BLAST
Transmembranei3748 – 376821HelicalSequence AnalysisAdd
BLAST
Transmembranei3775 – 379521HelicalSequence AnalysisAdd
BLAST
Transmembranei3802 – 382221HelicalSequence AnalysisAdd
BLAST
Transmembranei3846 – 386621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1083 – 1320238Peptidase C16 1PROSITE-ProRule annotationAdd
BLAST
Domaini1321 – 1481161MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1677 – 1936260Peptidase C16 2PROSITE-ProRule annotationAdd
BLAST
Domaini3337 – 3639303Peptidase C30PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2228 – 2465238HD1Add
BLAST
Regioni2846 – 3225380HD2Add
BLAST
Regioni3648 – 3866219HD3Add
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1197 – 122529C4-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1793 – 182937C4-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4397 – 441317By similarityAdd
BLAST
Zinc fingeri4439 – 445214By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR022570. Coronavirus_NSP1.
IPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_B_coronavir.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF11963. DUF3477. 2 hits.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1a (identifier: P0C6V1-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK
60 70 80 90 100
GKTLVNHVRV DCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG
110 120 130 140 150
SAVLVKPSKR LSVQAWAKLG VLPKTPAMGL FKRFCLCNTR ECVCDAHVAF
160 170 180 190 200
QLFTVQPDGV CLGNGRFIGW FVPVTAIPEY AKQWLQPWSI LLRKGGNKGS
210 220 230 240 250
VTSGHFRRAV TMPVYDFNVE DACEEVHLNP RGKYSCKAYA LLRGYRGVKP
260 270 280 290 300
ILFVDQYGCD YTGCLAKGLE DYGDLTLSEM KELSPVWRDS LDNEVVVAWH
310 320 330 340 350
VDRDPRAVMR LQTLATVRSI EYVGQPIEDM VDGDVVMREP AHLLAPNAIV
360 370 380 390 400
KRLPRLVETM LYTDSSVTEF CYKTKLCDCG FITQFGYVDC CGDTCGFRGW
410 420 430 440 450
VPGNMMDGFP CPGCCKSYMP WELEAQSSGV IPEGGVLFTQ STDTVNRESF
460 470 480 490 500
KLYGHAVVPF GGAAYWSPYP GMWLPVIWSS VKSYSYLTYT GVVGCKAIVQ
510 520 530 540 550
ETDAICRFLY MDYVQHKCGN LEQRAILGLD DVYHRQLLVN RGDYSLLLEN
560 570 580 590 600
VDLFVKRRAE FACKFATCGD GLVPLLLDGL VPRSYYLIKS GQAFTSLMVN
610 620 630 640 650
FSREVVDMCM DMALLFMHDV KVATKYVKKV TGKVAVRFKA LGIAVVRKIT
660 670 680 690 700
EWFDLAVDTA ASAAGWLCYQ LVNGLFAVAN GVITFIQEVP ELVKNFVDKF
710 720 730 740 750
KTFFKVLIDS MSVSILSGLT VVKTASNRVC LAGSKVYEVV QKSLPAYIMP
760 770 780 790 800
VGCSEATCLV GEIEPAVFED DVVDVVKAPL TYQGCCKPPS SFEKICIVDK
810 820 830 840 850
LYMAKCGDQF YPVVVDNDTV GVLDQCWRFP CAGKKVVFND KPKVKEVPST
860 870 880 890 900
RKIKIIFALD ATFDSVLSKA CSEFEVDKDV TLDELLDVVL DAVESTLSPC
910 920 930 940 950
KEHGVIGTKV CALLERLVDD YVYLFDEGGE EVIASRMYCS FSAPDEDCVA
960 970 980 990 1000
TDVVYADENQ DDDADDPVVL VADTQEEDGV AREQVDSADS EICVAHTGGQ
1010 1020 1030 1040 1050
EMTEPDVVGS QTPIASAEET EVGEACDREG IAEVKATVCA DALDACPDQV
1060 1070 1080 1090 1100
EAFDIEKVED SILSELQTEL NAPADKTYED VLAFDAIYSE TLSAFYAVPS
1110 1120 1130 1140 1150
DETHFKVCGF YSPAIERTNC WLRSTLIVMQ SLPLEFKDLG MQKLWLSYKA
1160 1170 1180 1190 1200
GYDQCFVDKL VKSAPKSIIL PQGGYVADFA YFFLSQCSFK VHANWRCLKC
1210 1220 1230 1240 1250
GMELKLQGLD AVFFYGDVVS HMCKCGNSMT LLSADIPYTF DFGVRDDKFC
1260 1270 1280 1290 1300
AFYTPRKVFR AACAVDVNDC HSMAVVDGKQ IDGKVVTKFN GDKFDFMVGH
1310 1320 1330 1340 1350
GMTFSMSPFE IAQLYGSCIT PNVCFVKGDV IKVLRRVGAE VIVNPANGRM
1360 1370 1380 1390 1400
AHGAGVAGAI AKAAGKAFIN ETADMVKAQG VCQVGGCYES TGGKLCKKVL
1410 1420 1430 1440 1450
NIVGPDARGH GNECYSLLER AYQHINKCDN VVTTLISAGI FSVPTDVSLT
1460 1470 1480 1490 1500
YLLGVVTKNV ILVSNNQDDF DVIEKCQVTS VAGTKALSFQ LAKNLCRDVK
1510 1520 1530 1540 1550
FVTNACSSLF SESSFVSSYD VLQEVEALRH DIQLDDDARV FVQANMDCLP
1560 1570 1580 1590 1600
TDWRLVNKFD SVDGVRTIKY FECPGEVFVS SQGKKFGYVQ NGSFKEASVS
1610 1620 1630 1640 1650
QIRALLANKV DVLCTVDGVN FRSCCVAEGE VFGKTLGSVF CDGINVTKVR
1660 1670 1680 1690 1700
CSAIHKGKVF FQYSGLSAAD LAAVKDAFGF DEPQLLQYYS MLGMCKWPVV
1710 1720 1730 1740 1750
VCGNYFAFKQ SNNNCYINVA CLMLQHLSLK FPKWQWRRPG NEFRSGKPLR
1760 1770 1780 1790 1800
FVSLVLAKGS FKFNEPSDST DFIRVELREA DLSGATCDLE FICKCGVKQE
1810 1820 1830 1840 1850
QRKGVDAVMH FGTLDKSGLV KGYNIACTCG DKLVHCTQFN VPFLICSNTP
1860 1870 1880 1890 1900
EGKKLPDDVV AANIFTGGSV GHYTHVKCKP KYQLYDACNV SKVSEAKGNF
1910 1920 1930 1940 1950
TDCLYLKNLK QTFSSVLTTY YLDDVKCVAY KPDLSQYYCE SGKYYTKPII
1960 1970 1980 1990 2000
KAQFRTFEKV EGVYTNFKLV GHDIAEKLNA KLGFDCNSPF MEYKITEWPT
2010 2020 2030 2040 2050
ATGDVVLASD DLYVSRYSGG CVTFGKPVIW RGHEEASLKS LTYFNRPSVV
2060 2070 2080 2090 2100
CENKFNVLPV DVSEPTDRRP VPSAVLVTGA ASGADASAIS TEPGTAKEQK
2110 2120 2130 2140 2150
ACASDSVEDQ IVMEAQKKSS VTTVAVKEVK LNGVKKPVKW NCSVVVNDPT
2160 2170 2180 2190 2200
SETKVVKSLS IVDVYDMFLT GCRYVVWTAN ELSRLINSPT VREYVKWGMS
2210 2220 2230 2240 2250
KLIIPANLLL LRDEKQEFVA PKVVKAKAIA CYGAVKWFLL YCFSWIKFNT
2260 2270 2280 2290 2300
DNKVIYTTEV ASKLTFKLCC LAFKNALQTF NWSVVSRGFF LVATVFLLWF
2310 2320 2330 2340 2350
NFLYANVILS DFYLPNIGPL PMFVGQIVAW VKTTFGVLTI CDFYQVTDLG
2360 2370 2380 2390 2400
YRSSFCNGSM VCELCFSGFD MLDNYESINV VQHVVDRRVS FDYISLFKLV
2410 2420 2430 2440 2450
VELVIGYSLY TVCFYPLFVL VGMQLLTTWL PEFFMLGTMH WSARLFVFVA
2460 2470 2480 2490 2500
NMLPAFTLLR FYIVVTAMYK VYCLCRHVMY GCSKPGCLFC YKRNRSVRVK
2510 2520 2530 2540 2550
CSTVVGGSLR YYDVMANGGT GFCTKHQWNC LNCNSWKPGN TFITHEAAAD
2560 2570 2580 2590 2600
LSKELKRPVN PTDSAYYSVI EVKQVGCSMR LFYERDGQRV YDDVSASLFV
2610 2620 2630 2640 2650
DMNGLLHSKV KGVPETHVVV VENEADKAGF LNAAVFYAQS LYRPMLMVEK
2660 2670 2680 2690 2700
KLITTANTGL SVSRTMFDLY VYSLLRHLDV DRKSLTSFVN AAHNSLKEGV
2710 2720 2730 2740 2750
QLEQVMDTFV GCARRKCAID SDVETKSITK SVMAAVNAGV EVTDESCNNL
2760 2770 2780 2790 2800
VPTYVKSDTI VAADLGVLIQ NNAKHVQSNV AKAANVACIW SVDAFNQLSA
2810 2820 2830 2840 2850
DLQHRLRKAC VKTGLKIKLT YNKQEANVPI LTTPFSLKGG AVFSRVLQWL
2860 2870 2880 2890 2900
FVANLICFIV LWALMPTYAV HKSDMQLPLY ASFKVIDNGV LRDVSVTDAC
2910 2920 2930 2940 2950
FANKFNQFDQ WYESTFGLVY YRNSKACPVV VAVIDQDIGH TLFNVPTKVL
2960 2970 2980 2990 3000
RYGFHVLHFI THAFATDRVQ CYTPHMQIPY DNFYASGCVL SSLCTMLAHA
3010 3020 3030 3040 3050
DGTPHPYCYT EGVMHNASLY SSLVPHVRYN LASSNGYIRF PEVVSEGIVR
3060 3070 3080 3090 3100
VVRTRSMTYC RVGLCEEAEE GICFNFNSSW VLNNPYYRAM PGTFCGRNAF
3110 3120 3130 3140 3150
DLIHQVLGGL VQPIDFFALT ASSVAGAILA IIVVLAFYYL IKLKRAFGDY
3160 3170 3180 3190 3200
TSVVVINVIV WCINFLMLFV FQVYPTLSCL YACFYFYTTL YFPSEISVVM
3210 3220 3230 3240 3250
HLQWLVMYGA IMPLWFCITY VAVVVSNHAL WLFSYCRKIG TDVRSDGTFE
3260 3270 3280 3290 3300
EMALTTFMIT KESYCKLKNS VSDVAFNRYL SLYNKYRYFS GKMDTATYRE
3310 3320 3330 3340 3350
AACSQLAKAM ETFNHNNGND VLYQPPTASV TTSFLQSGIV KMVSPTSKVE
3360 3370 3380 3390 3400
PCVVSVTYGN MTLNGLWLDD KVYCPRHVIC SSADMTDPDY PNLLCRVTSS
3410 3420 3430 3440 3450
DFCVMSDRMS LTVMSYQMQG SLLVLTVTLQ NPNTPKYSFG VVKPGETFTV
3460 3470 3480 3490 3500
LAAYNGRPQG AFHVVMRSSH TIKGSFLCGS CGSVGYVLTG DSVRFVYMHQ
3510 3520 3530 3540 3550
LELSTGCHTG TDFSGNFYGP YRDAQVVQLP VQDYTQTVNV VAWLYAAILN
3560 3570 3580 3590 3600
RCNWFVQSDS CSLEEFNVWA MTNGFSSIKA DLVLDALASM TGVTVEQVLA
3610 3620 3630 3640 3650
AIKRLHSGFQ GKQILGSCVL EDELTPSDVY QQLAGVKLQS KRTRVIKGTC
3660 3670 3680 3690 3700
CWILASTFLF CSIISAFVKW TMFMYVTTHM LGVTLCALCF VIFAMLLIKH
3710 3720 3730 3740 3750
KHLYLTMYIM PVLCTLFYTN YLVVGYKQSF RGLAYAWLSY FVPAVDYTYM
3760 3770 3780 3790 3800
DEVLYGVVLL VAMVFVTMRS INHDVFSTMF LVGRLVSLVS MWYFGANLEE
3810 3820 3830 3840 3850
EVLLFLTSLF GTYTWTTMLS LATAKVIAKW LAVNVLYFTD IPQIKLVLLS
3860 3870 3880 3890 3900
YLCIGYVCCC YWGVLSLLNS IFRMPLGVYN YKISVQELRY MNANGLRPPR
3910 3920 3930 3940 3950
NSFEALMLNF KLLGIGGVPV IEVSQIQSRL TDVKCANVVL LNCLQHLHIA
3960 3970 3980 3990 4000
SNSKLWQYCS TLHNEILATS DLSVAFDKLA QLLVVLFANP AAVDSKCLAS
4010 4020 4030 4040 4050
IEEVSDDYVR DNTVLQALQS EFVNMASFVE YELAKKNLDE AKASGSANQQ
4060 4070 4080 4090 4100
QIKQLEKACN IAKSAYERDR AVARKLERMA DLALTNMYKE ARINDKKSKV
4110 4120 4130 4140 4150
VSALQTMLFS MVRKLDNQAL NSILDNAVKG CVPLNAIPPL TSNTLTIIVP
4160 4170 4180 4190 4200
DKQVFDQVVD NVYVTYAPNV WHIQSIQDAD GAVKQLNEID VNSTWPLVIS
4210 4220 4230 4240 4250
ANRHNEVSTV VLQNNELMPQ KLRTQVVNSG SDMNCNIPTQ CYYNTTGTGK
4260 4270 4280 4290 4300
IVYAILSDCD GLKYTKIVKE DGNCVVLELD PPCKFSVQDV KGLKIKYLYF
4310 4320 4330 4340 4350
VKGCNTLARG WVVGTLSSTV RLQAGTATEY ASNSAILSLC AFSVDPKKTY
4360 4370 4380 4390 4400
LDYIQQGGVP VTNCVKMLCD HAGTGMAITI KPEATTNQDS YGGASVCIYC
4410 4420 4430 4440 4450
RSRVEHPDVD GLCKLRGKFV QVPLGIKDPV SYVLTHDVCQ VCGFWRDGSC
4460 4470
SCVGTGSQFQ SKDTNFLNGF GVQV

Note: Produced by conventional translation.

Length:4,474
Mass (Da):497,595
Last modified:June 10, 2008 - v1
Checksum:i7F09A6BF0E052D83
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6Y0-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

The sequence of this isoform can be found in the external entry P0C6Y0.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,180
Mass (Da):803,440
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55148 Genomic RNA. Translation: AAA46457.1.
M18040 Genomic RNA. Translation: AAA46466.1.
S51684 Genomic RNA. Translation: AAB19566.1.
PIRiA36815. RRIHM2.
B36815. VFIHJH.
RefSeqiYP_209230.1. AC_000192.1. [P0C6V1-1]

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55148 Genomic RNA. Translation: AAA46457.1 .
M18040 Genomic RNA. Translation: AAA46466.1 .
S51684 Genomic RNA. Translation: AAB19566.1 .
PIRi A36815. RRIHM2.
B36815. VFIHJH.
RefSeqi YP_209230.1. AC_000192.1. [P0C6V1-1 ]

3D structure databases

ProteinModelPortali P0C6V1.
SMRi P0C6V1. Positions 4055-4205, 4329-4453.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR022570. Coronavirus_NSP1.
IPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_B_coronavir.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR029063. SAM-dependent_MTases-like.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF11963. DUF3477. 2 hits.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SMARTi SM00506. A1pp. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEi PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase."
    Lee H.-J., Shieh C.-K., Gorbalenya A.E., Koonin E.V., la Monica N., Tuler J., Bagdzhardzhyan A., Lai M.M.C.
    Virology 180:567-582(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Sequence and translation of the murine coronavirus 5'-end genomic RNA reveals the N-terminal structure of the putative RNA polymerase."
    Soe L.H., Shieh C.-K., Baker S.C., Chang M.F., Lai M.M.C.
    J. Virol. 61:3968-3976(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-595.
  3. "Mouse hepatitis virus strain A59 RNA polymerase gene ORF 1a: heterogeneity among MHV strains."
    Bonilla P.J., Gorbalenya A.E., Weiss S.R.
    Virology 198:736-740(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  4. "Murine coronavirus gene 1 polyprotein contains an autoproteolytic activity."
    Baker S.C., La Monica N., Shieh C.K., Lai M.M.
    Adv. Exp. Med. Biol. 276:283-289(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1021-1326.
  5. "Identification of the murine coronavirus MP1 cleavage site recognized by papain-like proteinase 2."
    Kanjanahaluethai A., Jukneliene D., Baker S.C.
    J. Virol. 77:7376-7382(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, MUTAGENESIS OF PHE-2835; SER-2836; LEU-2837; LYS-2838; GLY-2839; GLY-2840; ALA-2841; VAL-2842 AND VAL-2846.
  6. "Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a."
    Schiller J.J., Kanjanahaluethai A., Baker S.C.
    Virology 242:288-302(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, SUBCELLULAR LOCATION.
  7. "Conservation of substrate specificities among coronavirus main proteases."
    Hegyi A., Ziebuhr J.
    J. Gen. Virol. 83:595-599(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN.

Entry informationi

Entry nameiR1A_CVMJH
AccessioniPrimary (citable) accession number: P0C6V1
Secondary accession number(s): P19751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3