Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0C6V0

- R1A_CVMA5

UniProt

P0C6V0 - R1A_CVMA5

Protein

Replicase polyprotein 1a

Gene

1a

Organism
Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.By similarity

    Catalytic activityi

    TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei247 – 2482Cleavage; by PL1-PROCurated
    Sitei832 – 8332Cleavage; by PL1-PROCurated
    Active sitei1121 – 11211For PL1-PRO activityPROSITE-ProRule annotation
    Active sitei1272 – 12721For PL1-PRO activityPROSITE-ProRule annotation
    Active sitei1716 – 17161For PL2-PRO activityPROSITE-ProRule annotation
    Active sitei1873 – 18731For PL2-PRO activityPROSITE-ProRule annotation
    Sitei2837 – 28382Cleavage; by PL2-PRO
    Sitei3333 – 33342Cleavage; by 3CL-PRO
    Active sitei3374 – 33741For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3478 – 34781For 3CL-PRO activity
    Sitei3635 – 36362Cleavage; by 3CL-PRO
    Sitei3921 – 39222Cleavage; by 3CL-PRO
    Sitei4013 – 40142Cleavage; by 3CL-PRO
    Sitei4207 – 42082Cleavage; by 3CL-PRO
    Sitei4317 – 43182Cleavage; by 3CL-PRO
    Sitei4454 – 44552Cleavage; by 3CL-PRO

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1198 – 122629C4-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1794 – 183037C4-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4391 – 440717By similarityAdd
    BLAST
    Zinc fingeri4433 – 444614By similarityAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: InterPro
    2. omega peptidase activity Source: InterPro
    3. RNA binding Source: UniProtKB-KW
    4. RNA-directed RNA polymerase activity Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    2. induction by virus of host autophagy Source: UniProtKB-KW
    3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    4. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    7. viral genome replication Source: InterPro
    8. viral protein processing Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1a
    Short name:
    pp1a
    Alternative name(s):
    ORF1a polyprotein
    Cleaved into the following 11 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    p28
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p65
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PL1/PL2
    Papain-like proteinases 1/2
    p210
    Non-structural protein 4
    Short name:
    nsp4
    Alternative name(s):
    Peptide HD2
    p44
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    M-PRO
    nsp5
    p27
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Alternative name(s):
    p10
    Non-structural protein 8
    Short name:
    nsp8
    Alternative name(s):
    p22
    Non-structural protein 9
    Short name:
    nsp9
    Alternative name(s):
    p12
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    p15
    Non-structural protein 11
    Short name:
    nsp11
    Gene namesi
    ORF Names:1a
    OrganismiMurine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
    Taxonomic identifieri11142 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]
    ProteomesiUP000007192: Genome

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.

    GO - Cellular componenti

    1. host cell membrane Source: UniProtKB-SubCell
    2. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi3331 – 33311F → A, H or W: No effect. 1 Publication
    Mutagenesisi3332 – 33321L → I or S: No processing between peptide HD2 and 3CL-PRO. 1 Publication
    Mutagenesisi3333 – 33331Q → A, K or R: No processing between peptide HD2 and 3CL-PRO. 1 Publication
    Mutagenesisi3334 – 33341S → A: No effect. 1 Publication
    Mutagenesisi3334 – 33341S → C: No processing between peptide HD2 and 3CL-PRO. 1 Publication
    Mutagenesisi3335 – 33351G → A: No effect. 1 Publication
    Mutagenesisi3335 – 33351G → P: No processing between peptide HD2 and 3CL-PRO. 1 Publication
    Mutagenesisi3336 – 33361I → L: No effect. 1 Publication
    Mutagenesisi3478 – 34781C → A: Complete loss of 3CL-PRO activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 44684468Replicase polyprotein 1aPRO_0000338278Add
    BLAST
    Chaini1 – 247247Non-structural protein 1CuratedPRO_0000338279Add
    BLAST
    Chaini248 – 832585Non-structural protein 2CuratedPRO_0000338280Add
    BLAST
    Chaini833 – 28372005Non-structural protein 3CuratedPRO_0000338281Add
    BLAST
    Chaini2838 – 3333496Non-structural protein 4PRO_0000338282Add
    BLAST
    Chaini3334 – 36353023C-like proteinasePRO_0000338283Add
    BLAST
    Chaini3636 – 3921286Non-structural protein 6CuratedPRO_0000338284Add
    BLAST
    Chaini3922 – 401392Non-structural protein 7PRO_0000338285Add
    BLAST
    Chaini4014 – 4207194Non-structural protein 8PRO_0000338286Add
    BLAST
    Chaini4208 – 4317110Non-structural protein 9PRO_0000338287Add
    BLAST
    Chaini4318 – 4454137Non-structural protein 10PRO_0000338288Add
    BLAST
    Chaini4455 – 446814Non-structural protein 11Sequence AnalysisPRO_0000338289Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    Secondary structure

    1
    4468
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi840 – 8423
    Beta strandi849 – 8535
    Beta strandi859 – 8613
    Helixi862 – 8709
    Beta strandi874 – 8774
    Helixi882 – 89615
    Helixi904 – 9074
    Helixi909 – 91810
    Beta strandi923 – 9308
    Beta strandi935 – 9428

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2M0ANMR-A833-946[»]
    ProteinModelPortaliP0C6V0.
    SMRiP0C6V0. Positions 4049-4199, 4323-4447.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2286 – 230621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2314 – 233421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2400 – 242021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2442 – 246221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2625 – 264521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2847 – 286721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3096 – 311621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3118 – 313821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3150 – 317021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3177 – 319721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3202 – 322221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3644 – 366421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3674 – 369421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3699 – 371921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3742 – 376221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3769 – 378921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3796 – 381621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3840 – 386021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1084 – 1333250Peptidase C16 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1323 – 1482160MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1678 – 1937260Peptidase C16 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini3334 – 3635302Peptidase C30PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2225 – 2645421HD1Add
    BLAST
    Regioni2847 – 3222376HD2Add
    BLAST
    Regioni3526 – 3860335HD3Add
    BLAST

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1198 – 122629C4-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1794 – 183037C4-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4391 – 440717By similarityAdd
    BLAST
    Zinc fingeri4433 – 444614By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    InterProiIPR022570. Coronavirus_NSP1.
    IPR002589. Macro_dom.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR002705. Pept_C30/C16_B_coronavir.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF11963. DUF3477. 2 hits.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF01831. Peptidase_C16. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SMARTiSM00506. A1pp. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEiPS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1a (identifier: P0C6V0-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK     50
    GKTLVNHVRV NCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG 100
    SAVLVKPSKR LSIQAWTNLG VLPKTAAMGL FKRVCLCNTR ECSCDAHVAF 150
    HLFTVQPDGV CLGNGRFIGW FVPVTAIPEY AKQWLQPWSI LLRKGGNKGS 200
    VTSGHFRRAV TMPVYDFNVE DACEEVHLNP KGKYSCKAYA LLKGYRGVKP 250
    ILFVDQYGCD YTGCLAKGLE DYGDLTLSEM KELFPVWRDS LDSEVLVAWH 300
    VDRDPRAAMR LQTLATVRCI DYVGQPTEDV VDGDVVVREP AHLLAANAIV 350
    KRLPRLVETM LYTDSSVTEF CYKTKLCECG FITQFGYVDC CGDTCDFRGW 400
    VAGNMMDGFP CPGCTKNYMP WELEAQSSGV IPEGGVLFTQ STDTVNRESF 450
    KLYGHAVVPF GSAVYWSPCP GMWLPVIWSS VKSYSGLTYT GVVGCKAIVQ 500
    ETDAICRSLY MDYVQHKCGN LEQRAILGLD DVYHRQLLVN RGDYSLLLEN 550
    VDLFVKRRAE FACKFATCGD GLVPLLLDGL VPRSYYLIKS GQAFTSMMVN 600
    FSHEVTDMCM DMALLFMHDV KVATKYVKKV TGKLAVRFKA LGVAVVRKIT 650
    EWFDLAVDIA ASAAGWLCYQ LVNGLFAVAN GVITFVQEVP ELVKNFVDKF 700
    KAFFKVLIDS MSVSILSGLT VVKTASNRVC LAGSKVYEVV QKSLSAYVMP 750
    VGCSEATCLV GEIEPAVFED DVVDVVKAPL TYQGCCKPPT SFEKICIVDK 800
    LYMAKCGDQF YPVVVDNDTV GVLDQCWRFP CAGKKVEFND KPKVRKIPST 850
    RKIKITFALD ATFDSVLSKA CSEFEVDKDV TLDELLDVVL DAVESTLSPC 900
    KEHDVIGTKV CALLDRLAGD YVYLFDEGGD EVIAPRMYCS FSAPDDEDCV 950
    AADVVDADEN QDDDAEDSAV LVADTQEEDG VAKGQVEADS EICVAHTGSQ 1000
    EELAEPDAVG SQTPIASAEE TEVGEASDRE GIAEAKATVC ADAVDACPDQ 1050
    VEAFEIEKVE DSILDELQTE LNAPADKTYE DVLAFDAVCS EALSAFYAVP 1100
    SDETHFKVCG FYSPAIERTN CWLRSTLIVM QSLPLEFKDL EMQKLWLSYK 1150
    AGYDQCFVDK LVKSVPKSII LPQGGYVADF AYFFLSQCSF KAYANWRCLE 1200
    CDMELKLQGL DAMFFYGDVV SHMCKCGNSM TLLSADIPYT LHFGVRDDKF 1250
    CAFYTPRKVF RAACAVDVND CHSMAVVEGK QIDGKVVTKF IGDKFDFMVG 1300
    YGMTFSMSPF ELAQLYGSCI TPNVCFVKGD VIKVVRLVNA EVIVNPANGR 1350
    MAHGAGVAGA IAEKAGSAFI KETSDMVKAQ GVCQVGECYE SAGGKLCKKV 1400
    LNIVGPDARG HGKQCYSLLE RAYQHINKCD NVVTTLISAG IFSVPTDVSL 1450
    TYLLGVVTKN VILVSNNQDD FDVIEKCQVT SVAGTKALSL QLAKNLCRDV 1500
    KFVTNACSSL FSESCFVSSY DVLQEVEALR HDIQLDDDAR VFVQANMDCL 1550
    PTDWRLVNKF DSVDGVRTIK YFECPGGIFV SSQGKKFGYV QNGSFKEASV 1600
    SQIRALLANK VDVLCTVDGV NFRSCCVAEG EVFGKTLGSV FCDGINVTKV 1650
    RCSAIYKGKV FFQYSDLSEA DLVAVKDAFG FDEPQLLKYY TMLGMCKWPV 1700
    VVCGNYFAFK QSNNNCYINV ACLMLQHLSL KFPKWQWQEA WNEFRSGKPL 1750
    RFVSLVLAKG SFKFNEPSDS IDFMRVVLRE ADLSGATCNL EFVCKCGVKQ 1800
    EQRKGVDAVM HFGTLDKGDL VRGYNIACTC GSKLVHCTQF NVPFLICSNT 1850
    PEGRKLPDDV VAANIFTGGS VGHYTHVKCK PKYQLYDACN VNKVSEAKGN 1900
    FTDCLYLKNL KQTFSSVLTT FYLDDVKCVE YKPDLSQYYC ESGKYYTKPI 1950
    IKAQFRTFEK VDGVYTNFKL VGHSIAEKLN AKLGFDCNSP FVEYKITEWP 2000
    TATGDVVLAS DDLYVSRYSS GCITFGKPVV WLGHEEASLK SLTYFNRPSV 2050
    VCENKFNVLP VDVSEPTDKG PVPAAVLVTG VPGADASAGA GIAKEQKACA 2100
    SASVEDQVVT EVRQEPSVSA ADVKEVKLNG VKKPVKVEGS VVVNDPTSET 2150
    KVVKSLSIVD VYDMFLTGCK YVVWTANELS RLVNSPTVRE YVKWGMGKIV 2200
    TPAKLLLLRD EKQEFVAPKV VKAKAIACYC AVKWFLLYCF SWIKFNTDNK 2250
    VIYTTEVASK LTFKLCCLAF KNALQTFNWS VVSRGFFLVA TVFLLWFNFL 2300
    YANVILSDFY LPNIGPLPTF VGQIVAWFKT TFGVSTICDF YQVTDLGYRS 2350
    SFCNGSMVCE LCFSGFDMLD NYDAINVVQH VVDRRLSFDY ISLFKLVVEL 2400
    VIGYSLYTVC FYPLFVLIGM QLLTTWLPEF FMLETMHWSA RLFVFVANML 2450
    PAFTLLRFYI VVTAMYKVYC LCRHVMYGCS KPGCLFCYKR NRSVRVKCST 2500
    VVGGSLRYYD VMANGGTGFC TKHQWNCLNC NSWKPGNTFI THEAAADLSK 2550
    ELKRPVNPTD SAYYSVTEVK QVGCSMRLFY ERDGQRVYDD VNASLFVDMN 2600
    GLLHSKVKGV PETHVVVVEN EADKAGFLGA AVFYAQSLYR PMLMVEKKLI 2650
    TTANTGLSVS RTMFDLYVDS LLNVLDVDRK SLTSFVNAAH NSLKEGVQLE 2700
    QVMDTFIGCA RRKCAIDSDV ETKSITKSVM SAVNAGVDFT DESCNNLVPT 2750
    YVKSDTIVAA DLGVLIQNNA KHVQANVAKA ANVACIWSVD AFNQLSADLQ 2800
    HRLRKACSKT GLKIKLTYNK QEANVPILTT PFSLKGGAVF SRMLQWLFVA 2850
    NLICFIVLWA LMPTYAVHKS DMQLPLYASF KVIDNGVLRD VSVTDACFAN 2900
    KFNQFDQWYE STFGLAYYRN SKACPVVVAV IDQDIGHTLF NVPTTVLRYG 2950
    FHVLHFITHA FATDSVQCYT PHMQIPYDNF YASGCVLSSL CTMLAHADGT 3000
    PHPYCYTGGV MHNASLYSSL APHVRYNLAS SNGYIRFPEV VSEGIVRVVR 3050
    TRSMTYCRVG LCEEAEEGIC FNFNRSWVLN NPYYRAMPGT FCGRNAFDLI 3100
    HQVLGGLVRP IDFFALTASS VAGAILAIIV VLAFYYLIKL KRAFGDYTSV 3150
    VVINVIVWCI NFLMLFVFQV YPTLSCLYAC FYFYTTLYFP SEISVVMHLQ 3200
    WLVMYGAIMP LWFCIIYVAV VVSNHALWLF SYCRKIGTEV RSDGTFEEMA 3250
    LTTFMITKES YCKLKNSVSD VAFNRYLSLY NKYRYFSGKM DTAAYREAAC 3300
    SQLAKAMETF NHNNGNDVLY QPPTASVTTS FLQSGIVKMV SPTSKVEPCI 3350
    VSVTYGNMTL NGLWLDDKVY CPRHVICSSA DMTDPDYPNL LCRVTSSDFC 3400
    VMSGRMSLTV MSYQMQGCQL VLTVTLQNPN TPKYSFGVVK PGETFTVLAA 3450
    YNGRPQGAFH VTLRSSHTIK GSFLCGSCGS VGYVLTGDSV RFVYMHQLEL 3500
    STGCHTGTDF SGNFYGPYRD AQVVQLPVQD YTQTVNVVAW LYAAIFNRCN 3550
    WFVQSDSCSL EEFNVWAMTN GFSSIKADLV LDALASMTGV TVEQVLAAIK 3600
    RLHSGFQGKQ ILGSCVLEDE TPSDVYQQLA GVKLQSKRTR VIKGTCCWIL 3650
    ASTFLFCSII SAFVKWTMFM YVTTHMLGVT LCALCFVSFA MLLIKHKHLY 3700
    LTMYIMPVLC TFYTNYLVVY KQSFRGLAYA WLSHFVPAVD YTYMDEVLYG 3750
    VVLLVAMVFV TMRSINHDVF SIMFLVGRLV SLVSMWYFGA NLEEEVLLFL 3800
    TSLFGTYTWT TMLSLATAKV IAKWLAVNVL YFTDVPQIKL VLLSYLCIGY 3850
    VCCCYWGILS LLNSIFRMPL GVYNYKISVQ ELRYMNANGL RPPRNSFEAL 3900
    MLNFKLLGIG GVPVIEVSQI QSRLTDVKCA NVVLLNCLQH LHIASNSKLW 3950
    QYCSTLHNEI LATSDLSMAF DKLAQLLVVL FANPAAVDSK CLASIEEVSD 4000
    DYVRDNTVLQ ALQSEFVNMA SFVEYELAKK NLDEAKASGS ANQQQIKQLE 4050
    KACNIAKSAY ERDRAVARKL ERMADLALTN MYKEARINDK KSKVVSALQT 4100
    MLFSMVRKLD NQALNSILDN AVKGCVPLNA IPSLTSNTLT IIVPDKQVFD 4150
    QVVDNVYVTY AGNVWHIQFI QDADGAVKQL NEIDVNSTWP LVIAANRHNE 4200
    VSTVVLQNNE LMPQKLRTQV VNSGSDMNCN TPTQCYYNTT GTGKIVYAIL 4250
    SDCDGLKYTK IVKEDGNCVV LELDPPCKFS VQDVKGLKIK YLYFVKGCNT 4300
    LARGWVVGTL SSTVRLQAGT ATEYASNSAI LSLCAFSVDP KKTYLDYIKQ 4350
    GGVPVTNCVK MLCDHAGTGM AITIKPEATT NQDSYGGASV CIYCRSRVEH 4400
    PDVDGLCKLR GKFVQVPLGI KDPVSYVLTH DVCQVCGFWR DGSCSCVGTG 4450
    SQFQSKDTNF LNGFGVQV 4468

    Note: Produced by conventional translation.

    Length:4,468
    Mass (Da):496,341
    Last modified:June 10, 2008 - v1
    Checksum:i53FB55E845B646A5
    GO
    Isoform Replicase polyprotein 1ab (identifier: P0C6X9-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    The sequence of this isoform can be found in the external entry P0C6X9.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:7,176
    Mass (Da):802,596
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti287 – 2882WR → CA(PubMed:2545027)Curated
    Sequence conflicti311 – 3111L → V(PubMed:2545027)Curated
    Sequence conflicti570 – 5701D → G(PubMed:2545027)Curated
    Sequence conflicti3620 – 36201E → EL(PubMed:9426441)Curated
    Sequence conflicti3711 – 37111T → TL(PubMed:9426441)Curated
    Sequence conflicti3968 – 39681M → V(PubMed:9426441)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1699 – 16991P → S in strain: Isolate C12 mutant.
    Natural varianti2196 – 21961M → K in strain: Isolate C12 mutant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73559 Genomic RNA. No translation available.
    AF029248 Genomic RNA. Translation: AAB86820.1.
    M27198 Genomic RNA. Translation: AAA74011.1.
    PIRiA32440.
    S15760.
    RefSeqiNP_045298.1. NC_001846.1.

    Genome annotation databases

    GeneIDi1489749.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73559 Genomic RNA. No translation available.
    AF029248 Genomic RNA. Translation: AAB86820.1 .
    M27198 Genomic RNA. Translation: AAA74011.1 .
    PIRi A32440.
    S15760.
    RefSeqi NP_045298.1. NC_001846.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2M0A NMR - A 833-946 [» ]
    ProteinModelPortali P0C6V0.
    SMRi P0C6V0. Positions 4049-4199, 4323-4447.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1489749.

    Family and domain databases

    InterProi IPR022570. Coronavirus_NSP1.
    IPR002589. Macro_dom.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR002705. Pept_C30/C16_B_coronavir.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR029063. SAM-dependent_MTases-like.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF11963. DUF3477. 2 hits.
    PF01661. Macro. 1 hit.
    PF09401. NSP10. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF01831. Peptidase_C16. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view ]
    SMARTi SM00506. A1pp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF53335. SSF53335. 1 hit.
    PROSITEi PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse hepatitis virus strain A59 RNA polymerase gene ORF 1a: heterogeneity among MHV strains."
      Bonilla P.J., Gorbalenya A.E., Weiss S.R.
      Virology 198:736-740(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein."
      Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.
      Virology 239:1-10(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate C12 mutant.
    3. "Molecular cloning of the gene encoding the putative polymerase of mouse hepatitis coronavirus, strain A59."
      Pachuk C.J., Bredenbeek P.J., Zoltick P.W., Spaan W.J.M., Weiss S.R.
      Virology 171:141-148(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-597.
    4. "Mouse hepatitis virus 3C-like protease cleaves a 22-kilodalton protein from the open reading frame 1a polyprotein in virus-infected cells and in vitro."
      Lu X.T., Sims A.C., Denison M.R.
      J. Virol. 72:2265-2271(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, CHARACTERIZATION OF NSP8.
    5. "Further requirements for cleavage by the murine coronavirus 3C-like proteinase: identification of a cleavage site within ORF1b."
      Pinon J.D., Teng H., Weiss S.R.
      Virology 263:471-484(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, MUTAGENESIS OF PHE-3331; LEU-3332; GLN-3333; SER-3334; GLY-3335; ILE-3336 AND CYS-3478.
    6. "Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly."
      Bost A.G., Carnahan R.H., Lu X.T., Denison M.R.
      J. Virol. 74:3379-3387(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, CHARACTERIZATION OF NSP7; NSP9 AND NSP10.

    Entry informationi

    Entry nameiR1A_CVMA5
    AccessioniPrimary (citable) accession number: P0C6V0
    Secondary accession number(s): P16342
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3