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P0C6U7

- R1A_CVHOC

UniProt

P0C6U7 - R1A_CVHOC

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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Human coronavirus OC43 (HCoV-OC43)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei246 – 2472Cleavage; by PL1-PROBy similarity
Sitei851 – 8522Cleavage; by PL1-PROBy similarity
Active sitei1074 – 10741For PL1-PRO activityPROSITE-ProRule annotation
Active sitei1225 – 12251For PL1-PRO activityPROSITE-ProRule annotation
Active sitei1671 – 16711For PL2-PRO activityPROSITE-ProRule annotation
Active sitei1828 – 18281For PL2-PRO activityPROSITE-ProRule annotation
Sitei2750 – 27512Cleavage; by PL2-PROBy similarity
Sitei3246 – 32472Cleavage; by 3CL-PROBy similarity
Active sitei3287 – 32871For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3391 – 33911For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3549 – 35502Cleavage; by 3CL-PROBy similarity
Sitei3836 – 38372Cleavage; by 3CL-PROBy similarity
Sitei3925 – 39262Cleavage; by 3CL-PROBy similarity
Sitei4122 – 41232Cleavage; by 3CL-PROBy similarity
Sitei4232 – 42332Cleavage; by 3CL-PROBy similarity
Sitei4369 – 43702Cleavage; by 3CL-PROBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1151 – 117929C4-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1749 – 178537C4-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4306 – 432217By similarityAdd
BLAST
Zinc fingeri4348 – 436114By similarityAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA-directed RNA polymerase activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. viral genome replication Source: InterPro
  8. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p28
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65
Alternative name(s):
PL1-PRO/PL2-PRO
PL1/PL2
Papain-like proteinases 1/2
p210
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
p44
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p27
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p10
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p22
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p15
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiHuman coronavirus OC43 (HCoV-OC43)
Taxonomic identifieri31631 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007552: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 43834383Replicase polyprotein 1aPRO_0000338242Add
BLAST
Chaini1 – 246246Non-structural protein 1By similarityPRO_0000338243Add
BLAST
Chaini247 – 851605Non-structural protein 2By similarityPRO_0000338244Add
BLAST
Chaini852 – 27501899Non-structural protein 3By similarityPRO_0000338245Add
BLAST
Chaini2751 – 3246496Non-structural protein 4By similarityPRO_0000338246Add
BLAST
Chaini3247 – 35493033C-like proteinaseBy similarityPRO_0000338247Add
BLAST
Chaini3550 – 3836287Non-structural protein 6By similarityPRO_0000338248Add
BLAST
Chaini3837 – 392589Non-structural protein 7By similarityPRO_0000338249Add
BLAST
Chaini3926 – 4122197Non-structural protein 8By similarityPRO_0000338250Add
BLAST
Chaini4123 – 4232110Non-structural protein 9By similarityPRO_0000338251Add
BLAST
Chaini4233 – 4369137Non-structural protein 10By similarityPRO_0000338252Add
BLAST
Chaini4370 – 438314Non-structural protein 11Sequence AnalysisPRO_0000338253Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6U7.
SMRiP0C6U7. Positions 3964-4114, 4238-4362.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2138 – 215821HelicalSequence AnalysisAdd
BLAST
Transmembranei2199 – 221921HelicalSequence AnalysisAdd
BLAST
Transmembranei2221 – 224121HelicalSequence AnalysisAdd
BLAST
Transmembranei2313 – 233321HelicalSequence AnalysisAdd
BLAST
Transmembranei2343 – 236321HelicalSequence AnalysisAdd
BLAST
Transmembranei2365 – 238521HelicalSequence AnalysisAdd
BLAST
Transmembranei2752 – 277221HelicalSequence AnalysisAdd
BLAST
Transmembranei2824 – 284421HelicalSequence AnalysisAdd
BLAST
Transmembranei3009 – 302921HelicalSequence AnalysisAdd
BLAST
Transmembranei3031 – 305121HelicalSequence AnalysisAdd
BLAST
Transmembranei3063 – 308321HelicalSequence AnalysisAdd
BLAST
Transmembranei3090 – 311021HelicalSequence AnalysisAdd
BLAST
Transmembranei3115 – 313521HelicalSequence AnalysisAdd
BLAST
Transmembranei3558 – 357821HelicalSequence AnalysisAdd
BLAST
Transmembranei3588 – 360821HelicalSequence AnalysisAdd
BLAST
Transmembranei3615 – 363521HelicalSequence AnalysisAdd
BLAST
Transmembranei3657 – 367721HelicalSequence AnalysisAdd
BLAST
Transmembranei3684 – 370421HelicalSequence AnalysisAdd
BLAST
Transmembranei3711 – 373121HelicalSequence AnalysisAdd
BLAST
Transmembranei3755 – 377521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1036 – 1274239Peptidase C16 1PROSITE-ProRule annotationAdd
BLAST
Domaini1275 – 1435161MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1631 – 1892262Peptidase C16 2PROSITE-ProRule annotationAdd
BLAST
Domaini3247 – 3549303Peptidase C30PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2138 – 2385248HD1By similarityAdd
BLAST
Regioni2752 – 3135384HD2By similarityAdd
BLAST
Regioni3319 – 3775457HD3By similarityAdd
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1151 – 117929C4-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1749 – 178537C4-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4306 – 432217By similarityAdd
BLAST
Zinc fingeri4348 – 436114By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR022570. Coronavirus_NSP1.
IPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_B_coronavir.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF11963. DUF3477. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1a (identifier: P0C6U7-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MSKINKYGLE LHWAPEFPWM FEDAEEKLDN PSSSEVDMIC STTAQKLETD
60 70 80 90 100
GICPENHVMV DCRRLLKQEC CVQSSLIREI VMNASPYHLE VLLQDALQSR
110 120 130 140 150
EAVLVTTPLG MSLEACYVRG CNPKGWTMGL FRRRSVCNTG RCTVNKHVAY
160 170 180 190 200
QLYMIDPAGV CLGAGQFVGW VIPLAFMPVQ SRKFIVPWVM YLRKRGEKGA
210 220 230 240 250
YNKDHGCGGF GHVYDFKVED AYDQVHDEPK GKFSKKAYAL IRGYRGVKPL
260 270 280 290 300
LYVDQYGCDY TGSLADGLEA YADKTLQEMK ALFPTWSQEL PFDVIVAWHV
310 320 330 340 350
VRDPRYVMRL QSAATICSVA YVANPTEDLC DGSVVIKEPV HVYADDSIIL
360 370 380 390 400
RQYNLFDIMS HFYMEADTVV NAFYGVALKD CGFVMQFGYI DCEQDSCDFK
410 420 430 440 450
GWIPGNMIDG FACTTCGHVY EVGDLIAQSS GVLPVNPVLH TKSAAGYGGF
460 470 480 490 500
GCKDSFTLYG QTVVYFGGCV YWSPARNIWI PILKSSVKSY DSLVYTGVLG
510 520 530 540 550
CKAIVKETNL ICKALYLDYV QHKCGNLHQR ELLGVSDVWH KQLLINRGVY
560 570 580 590 600
KPLLENIDYF NMRRAKFSLE TFTVCADGFM PFLLDDLVPR AYYLAVSGQA
610 620 630 640 650
FCDYADKLCH AVVSKSKELL DVSLDSLGAA IHYLNSKIVD LAQHFSDFGT
660 670 680 690 700
SFVSKIVHFF KTFTTSTALA FAWVLFHVLH GAYIVVESDI YFVKNIPRYA
710 720 730 740 750
SAVAQAFQSV AKVVLDSLRV TFIDGLSCFK IGRRRICLSG RKIYEVERGL
760 770 780 790 800
LHSSQLPLDV YDLTMPSQVQ KAKQKPIYLK GSGSDFSLAD SVVEVVTTSL
810 820 830 840 850
TPCGYSEPPK VADKICIVDN VYMAKAGDKY YPVVVDDHVG LLDQAWRVPC
860 870 880 890 900
AGRRVTFKEQ PTVKEIISMP KIIKVFYELD NDFNTILNTA CGVFEVDDTV
910 920 930 940 950
DMEEFYAVVI DAIEEKLSPC KELEGVGAKV SAFLQKLEDN PLFLFDEAGE
960 970 980 990 1000
EVFAPKLYCA FTAPEDDDFL EESDVEEDDV EGEETDLTIT SAGQPCVASE
1010 1020 1030 1040 1050
QEESSEVLED TLDDGPSVET SDSQVEEDVE MSDFVDLESV IQDYENVCFE
1060 1070 1080 1090 1100
FYTTEPEFVK VLGLYVPKAT RNNCWLRSVL AVMQKLPCQF KDKNLQDLWV
1110 1120 1130 1140 1150
LYKQQYSQLF VDTLVNKIPA NIVLPQGGYV ADFAYWFLTL CDWQCVAYWK
1160 1170 1180 1190 1200
CIKCDLALKL KGLDAMFFYG DVVSHICKCG ESMVLIDVDV PFTAHFALKD
1210 1220 1230 1240 1250
KLFCAFITKR IVYKAACVVD VNDSHSMAVV DGKQIDDHRI TSITSDKFDF
1260 1270 1280 1290 1300
IIGHGMSFSM TTFEIAQLYG SCITPNVCFV KGDIIKVSKL VKAEVVVNPA
1310 1320 1330 1340 1350
NGHMVHGGGV AKAIAVAAGQ QFVKETTNMV KSKGVCATGD CYVSTGGKLC
1360 1370 1380 1390 1400
KTVLNVVGPD ARTQGKQSYV LLERVYKHFN NYDCVVTTLI SAGIFSVPSD
1410 1420 1430 1440 1450
VSLTYLLGTA KKQVVLVSNN QEDFDLISKC QITAVEGTKK LAARLSFNVG
1460 1470 1480 1490 1500
RSIVYETDAN KLILINDVAF VSTFNVLQDV LSLRHDIALD DDARTFVQSN
1510 1520 1530 1540 1550
VDVLPEGWRV VNKFYQINGV RTVKYFECTG GIDICSQDKV FGYVQQGIFN
1560 1570 1580 1590 1600
KATVAQIKAL FLDKVDILLT VDGVNFTNRF VPVGESFGKS LGNVFCDGVN
1610 1620 1630 1640 1650
VTKHKCDINY KGKVFFQFDN LSSEDLKAVR SSFNFDQKEL LAYYNMLVNC
1660 1670 1680 1690 1700
FKWQVVVNGK YFTFKQANNN CFVNVSCLML QSLHLTFKIV QWQEAWLEFR
1710 1720 1730 1740 1750
SGRPARFVAL VLAKGGFKFG DPADSRDFLR VVFSQVDLTG AICDFEIACK
1760 1770 1780 1790 1800
CGVKQEQRTG LDAVMHFGTL SREDLEIGYT VDCSCGKKLI HCVRFDVPFL
1810 1820 1830 1840 1850
ICSNTPASVK LPKGVGSANI FIGDNVGHYV HVKCEQSYQL YDASNVKKVT
1860 1870 1880 1890 1900
DVTGKLSDCL YLKNLKQTFK SVLTTYYLDD VKKIEYKPDL SQYYCDGGKY
1910 1920 1930 1940 1950
YTQRIIKAQF KTFEKVDGVY TNFKLIGHTV CDSLNSKLGF DSSKEFVEYK
1960 1970 1980 1990 2000
ITEWPTATGD VVLANDDLYV KRYERGCITF GKPVIWLSHE KASLNSLTYF
2010 2020 2030 2040 2050
NRPLLVDDNK FDVLKVDDVD DSGDSSESGA KETKEINIIK LSGVKKPFKV
2060 2070 2080 2090 2100
EDSVIVNDDT SETKYVKSLS IVDVYDMWLT GCKYVVRTAN ALSRAVNVPT
2110 2120 2130 2140 2150
IRKFIKFGMT LVSIPIDLLN LREIKPAVNV VKAVRNKTSA CFNFIKWLFV
2160 2170 2180 2190 2200
LLFGWIKISA DNKVIYTTEI ASKLTCKLVA LAFKNAFLTF KWSMVARGAC
2210 2220 2230 2240 2250
IIATIFLLWF NFIYANVIFS DFYLPKIGFL PTFVGKIAQW IKNTFSLVTI
2260 2270 2280 2290 2300
CDLYSIQDVG FKNQYCNGSI ACQFCLAGFD MLDNYKAIDV VQYEADRRAF
2310 2320 2330 2340 2350
VDYTGVLKIV IELIVSYALY TAWFYPLFAL ISIQILTTWL PELFMLSTLH
2360 2370 2380 2390 2400
WSFRLLVALA NMLPAHVFMR FYIIIASFIK LFSLFKHVAY GCSKSGCLFC
2410 2420 2430 2440 2450
YKRNRSLRVK CSTIVGGMIR YYDVMANGGT GFCSKHQWNC IDCDSYKPGN
2460 2470 2480 2490 2500
TFITVEAALD LSKELKRPIQ PTDVAYHTVT DVKQVGCSMR LFYDRDGQRI
2510 2520 2530 2540 2550
YDDVNASLFV DYSNLLHSKV KSVPNMHVVV VENDADKANF LNAAVFYAQS
2560 2570 2580 2590 2600
LFRPILMVDK NLITTANTGT SVTETMFDVY VDTFLSMFDV DKKSLNALIA
2610 2620 2630 2640 2650
TAHSSIKQGT QIYKVLDTFL SCARKSCSID SDVDTKCLAD SVMSAVSAGL
2660 2670 2680 2690 2700
ELTDESCNNL VPTYLKSDNI VAADLGVLIQ NSAKHVQGNV AKIAGVSCIW
2710 2720 2730 2740 2750
SVDAFNQFSS DFQHKLKKAC CKTGLKLKLT YNKQMANVSV LTTPFSLKGG
2760 2770 2780 2790 2800
AVFSYFVYVC FVLSLVCFIG LWCLMPTYTV HKSDFQLPVY ASYKVLDNGV
2810 2820 2830 2840 2850
IRDVSVEDVC FANKFEQFDQ WYESTFGLSY YSNSMACPIV VAVIDQDFGS
2860 2870 2880 2890 2900
TVFNVPTKVL RYGYHVLHFI THALSADGVQ CYTPHSQISY SNFYASGCVL
2910 2920 2930 2940 2950
SSACTMFTMA DGSPQPYCYT DGLMQNASLY SSLVPHVRYN LANAKGFIRF
2960 2970 2980 2990 3000
PEVLREGLVR VVRTRSMSYC RVGLCEEADE GICFNFNGSW VLNNDYYRSL
3010 3020 3030 3040 3050
PGTFCGRDVF DLIYQLFKGL AQPVDFLALT ASSIAGAILA VIVVLVFYYL
3060 3070 3080 3090 3100
IKLKRAFGDY TSVVFVNVIV WCVNFMMLFV FQVYPTLSCV YAICYFYATL
3110 3120 3130 3140 3150
YFPSEISVIM HLQWLVMYGT IMPLWFCLLY IAVVVSNHAF WVFSYCRKLG
3160 3170 3180 3190 3200
TSVRSDGTFE EMALTTFMIT KDSYCKLKNS LSDVAFNRYL SLYNKYRYYS
3210 3220 3230 3240 3250
GKMDTAAYRE AACSQLAKAM DTFTNNNGSD VLYQPPTASV STSFLQSGIV
3260 3270 3280 3290 3300
KMVNPTSKVE PCVVSVTYGN MTLNGLWLDD KVYCPRHVIC SASDMTNPDY
3310 3320 3330 3340 3350
TNLLCRVTSS DFTVLFDRLS LTVMSYQMRG CMLVLTVTLQ NSRTPKYTFG
3360 3370 3380 3390 3400
VVKPGETFTV LAAYNGKPQG AFHVTMRSSY TIKGSFLCGS CGSVGYVIMG
3410 3420 3430 3440 3450
DCVKFVYMHQ LELSTGCHTG TDFNGDFYGP YKDAQVVQLP IQDYIQSVNF
3460 3470 3480 3490 3500
LAWLYAAILN NCNWFIQSDK CSVEDFNVWA LSNGFSQVKS DLVIDALASM
3510 3520 3530 3540 3550
TGVSLETLLA AIKRLKNGFQ GRQIMGSCSF EDELTPSDVY QQLAGIKLQS
3560 3570 3580 3590 3600
KRTRLFKGTV CWIMASTFLF SCIITAFVKW TMFMYVTTNM FSITFCALCV
3610 3620 3630 3640 3650
ISLAMLLVKH KHLYLTMYIT PVLFTLLYNN YLVVYKHTFR GYVYAWLSYY
3660 3670 3680 3690 3700
VPSVEYTYTD EVIYGMLLLV GMVFVTLRSI NHDLFSFIMF VGRLISVFSL
3710 3720 3730 3740 3750
WYKGSNLEEE ILLMLASLFG TYTWTTVLSM AVAKVIAKWV AVNVLYFTDI
3760 3770 3780 3790 3800
PQIKIVLLCY LFIGYIISCY WGLFSLMNSL FRMPLGVYNY KISVQELRYM
3810 3820 3830 3840 3850
NANGLRPPKN SFEALMLNFK LLGIGGVPII EVSQFQSKLT DVKCANVVLL
3860 3870 3880 3890 3900
NCLQHLHVAS NSKLWHYCST LHNEILATSD LSVAFEKLAQ LLIVLFANPA
3910 3920 3930 3940 3950
AVDSKCLTSI EEVCDDYAKD NTVLQALQSE FVNMASFVEY EVAKKNLDEA
3960 3970 3980 3990 4000
RFSGSANQQQ LKQLEKACNI AKSAYERDRA VAKKLERMAD LALTNMYKEA
4010 4020 4030 4040 4050
RINDKKSKVV SALQTMLFSM VRKLDNQALN SILDNAVKGC VPLNAIPSLA
4060 4070 4080 4090 4100
ANTLNIIVPD KSVYDQIVDN IYVTYAGNVW QIQTIQDSDG TNKQLNEISD
4110 4120 4130 4140 4150
DCNWPLVIIA NRYNEVSATV LQNNELMPAK LKIQVVNSGP DQTCNTPTQC
4160 4170 4180 4190 4200
YYNNSNNGKI VYAILSDVDG LKYTKILKDD GNFVVLELDP PCKFTVQDAK
4210 4220 4230 4240 4250
GLKIKYLYFV KGCNTLARGW VVGTISSTVR LQAGTATEYA SNSSILSLCA
4260 4270 4280 4290 4300
FSVDPKKTYL DFIQQGGTPI ANCVKMLCDH AGTGMAITVK PDATTSQDSY
4310 4320 4330 4340 4350
GGASVCIYCR ARVEHPDVDG LCKLRGKFVQ VPVGIKDPVS YVLTHDVCRV
4360 4370 4380
CGFWRDGSCS CVSTDTTVQS KDTNFLNGFG VRV

Note: Produced by conventional translation.

Length:4,383
Mass (Da):491,305
Last modified:June 10, 2008 - v1
Checksum:i264040B0DDCDF54E
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6X6-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

The sequence of this isoform can be found in the external entry P0C6X6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,095
Mass (Da):797,914
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti426 – 4261I → M(PubMed:15650185)Curated
Sequence conflicti813 – 8131D → A(PubMed:15650185)Curated
Sequence conflicti4376 – 43772LN → FK(PubMed:15280490)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti88 – 881H → D in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti207 – 2071C → R in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti291 – 2911P → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti317 – 3171C → R in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti356 – 3561F → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti545 – 5451I → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti762 – 7621D → N in strain: Isolate 87309 Belgium 2003.
Natural varianti953 – 9531F → L in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural varianti989 – 9891I → V in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural varianti1305 – 13051V → A in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural varianti1328 – 13281N → D in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti1379 – 13791F → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti1504 – 15041L → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti1740 – 17401G → E in strain: Isolate 87309 Belgium 2003.
Natural varianti1825 – 18251N → K in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti1936 – 19361S → A in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural varianti1965 – 19651N → T in strain: ATCC VR-759, Isolate clinical OC43-Paris.
Natural varianti2004 – 20041L → S in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural varianti2022 – 20221S → G in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti2138 – 21403TSA → ISV in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti2256 – 22561I → M in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti2257 – 22571Q → H in strain: Isolate 87309 Belgium 2003.
Natural varianti2386 – 23861K → R in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti2500 – 25001I → T in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural varianti2921 – 29211D → E in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti2961 – 29611V → I in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti3086 – 30861T → I in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti3440 – 34401P → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti3451 – 34511L → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti3466 – 34661I → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti4067 – 40671I → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural varianti4071 – 40711I → V in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY903459 Genomic RNA. No translation available.
AY903460 Genomic RNA. No translation available.
AY585228 Genomic RNA. No translation available.
AY585229 Genomic RNA. No translation available.
AY391777 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY903459 Genomic RNA. No translation available.
AY903460 Genomic RNA. No translation available.
AY585228 Genomic RNA. No translation available.
AY585229 Genomic RNA. No translation available.
AY391777 Genomic RNA. No translation available.

3D structure databases

ProteinModelPortali P0C6U7.
SMRi P0C6U7. Positions 3964-4114, 4238-4362.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR022570. Coronavirus_NSP1.
IPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_B_coronavir.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF11963. DUF3477. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SMARTi SM00506. A1pp. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEi PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Circulation of genetically distinct contemporary human coronavirus OC43 strains."
    Vijgen L., Keyaerts E., Lemey P., Moes E., Li S., Vandamme A.M., Van Ranst M.
    Virology 337:85-92(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate 19572 Belgium 2004 and Isolate 87309 Belgium 2003.
  2. "Human respiratory coronavirus OC43: genetic stability and neuroinvasion."
    St Jean J.R., Jacomy H., Desforges M., Vabret A., Freymuth F., Talbot P.J.
    J. Virol. 78:8824-8834(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.
  3. "Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event."
    Vijgen L., Keyaerts E., Moes E., Thoelen I., Wollants E., Lemey P., Vandamme A.M., Van Ranst M.
    J. Virol. 79:1595-1604(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate ATCC VR-759.

Entry informationi

Entry nameiR1A_CVHOC
AccessioniPrimary (citable) accession number: P0C6U7
Secondary accession number(s): Q9WAC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence shown is that of isolate 19572 Belgium 2004.

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3