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P0C6U7 (R1A_CVHOC) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1a

Short name=pp1a
Alternative name(s):
ORF1a polyprotein

Cleaved into the following 11 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p28
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PL1/PL2
    Papain-like proteinases 1/2
    p210
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
    p44
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p27
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p10
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p22
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p15
  11. Non-structural protein 11
    Short name=nsp11
Gene names
ORF Names:1a
OrganismHuman coronavirus OC43 (HCoV-OC43) [Complete proteome]
Taxonomic identifier31631 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length4383 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activity

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Miscellaneous

The sequence shown is that of isolate 19572 Belgium 2004.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Eukaryotic host gene expression shutoff by virus
Eukaryotic host translation shutoff by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host IRF3 by virus
Inhibition of host ISG15 by virus
Inhibition of host RLR pathway by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandMetal-binding
RNA-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host IRF3 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

viral genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: InterPro

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1a (identifier: P0C6U7-1)

Also known as: pp1a; ORF1a polyprotein;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6X6-1)

Also known as: pp1ab;

The sequence of this isoform can be found in the external entry P0C6X6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 43834383Replicase polyprotein 1a
PRO_0000338242
Chain1 – 246246Non-structural protein 1 By similarity
PRO_0000338243
Chain247 – 851605Non-structural protein 2 By similarity
PRO_0000338244
Chain852 – 27501899Non-structural protein 3 By similarity
PRO_0000338245
Chain2751 – 3246496Non-structural protein 4 By similarity
PRO_0000338246
Chain3247 – 35493033C-like proteinase By similarity
PRO_0000338247
Chain3550 – 3836287Non-structural protein 6 By similarity
PRO_0000338248
Chain3837 – 392589Non-structural protein 7 By similarity
PRO_0000338249
Chain3926 – 4122197Non-structural protein 8 By similarity
PRO_0000338250
Chain4123 – 4232110Non-structural protein 9 By similarity
PRO_0000338251
Chain4233 – 4369137Non-structural protein 10 By similarity
PRO_0000338252
Chain4370 – 438314Non-structural protein 11 Potential
PRO_0000338253

Regions

Transmembrane2138 – 215821Helical; Potential
Transmembrane2199 – 221921Helical; Potential
Transmembrane2221 – 224121Helical; Potential
Transmembrane2313 – 233321Helical; Potential
Transmembrane2343 – 236321Helical; Potential
Transmembrane2365 – 238521Helical; Potential
Transmembrane2752 – 277221Helical; Potential
Transmembrane2824 – 284421Helical; Potential
Transmembrane3009 – 302921Helical; Potential
Transmembrane3031 – 305121Helical; Potential
Transmembrane3063 – 308321Helical; Potential
Transmembrane3090 – 311021Helical; Potential
Transmembrane3115 – 313521Helical; Potential
Transmembrane3558 – 357821Helical; Potential
Transmembrane3588 – 360821Helical; Potential
Transmembrane3615 – 363521Helical; Potential
Transmembrane3657 – 367721Helical; Potential
Transmembrane3684 – 370421Helical; Potential
Transmembrane3711 – 373121Helical; Potential
Transmembrane3755 – 377521Helical; Potential
Domain1036 – 1274239Peptidase C16 1
Domain1275 – 1435161Macro
Domain1631 – 1892262Peptidase C16 2
Domain3247 – 3549303Peptidase C30
Zinc finger1151 – 117929C4-type 1 By similarity
Zinc finger1749 – 178537C4-type 2 By similarity
Zinc finger4306 – 432217 By similarity
Zinc finger4348 – 436114 By similarity
Region2138 – 2385248HD1 By similarity
Region2752 – 3135384HD2 By similarity
Region3319 – 3775457HD3 By similarity

Sites

Active site10741For PL1-PRO activity By similarity
Active site12251For PL1-PRO activity By similarity
Active site16711For PL2-PRO activity By similarity
Active site18281For PL2-PRO activity By similarity
Active site32871For 3CL-PRO activity By similarity
Active site33911For 3CL-PRO activity By similarity
Site246 – 2472Cleavage; by PL1-PRO By similarity
Site851 – 8522Cleavage; by PL1-PRO By similarity
Site2750 – 27512Cleavage; by PL2-PRO By similarity
Site3246 – 32472Cleavage; by 3CL-PRO By similarity
Site3549 – 35502Cleavage; by 3CL-PRO By similarity
Site3836 – 38372Cleavage; by 3CL-PRO By similarity
Site3925 – 39262Cleavage; by 3CL-PRO By similarity
Site4122 – 41232Cleavage; by 3CL-PRO By similarity
Site4232 – 42332Cleavage; by 3CL-PRO By similarity
Site4369 – 43702Cleavage; by 3CL-PRO By similarity

Natural variations

Natural variant881H → D in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant2071C → R in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant2911P → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant3171C → R in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant3561F → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant5451I → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant7621D → N in strain: Isolate 87309 Belgium 2003.
Natural variant9531F → L in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural variant9891I → V in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural variant13051V → A in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural variant13281N → D in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant13791F → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant15041L → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant17401G → E in strain: Isolate 87309 Belgium 2003.
Natural variant18251N → K in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant19361S → A in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural variant19651N → T in strain: ATCC VR-759, Isolate clinical OC43-Paris.
Natural variant20041L → S in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural variant20221S → G in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant2138 – 21403TSA → ISV in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant22561I → M in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant22571Q → H in strain: Isolate 87309 Belgium 2003.
Natural variant23861K → R in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant25001I → T in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.
Natural variant29211D → E in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant29611V → I in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant30861T → I in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant34401P → L in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant34511L → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant34661I → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant40671I → V in strain: ATCC VR-759 and Isolate clinical OC43-Paris.
Natural variant40711I → V in strain: ATCC VR-759, Isolate clinical OC43-Paris and Isolate 87309 Belgium 2003.

Experimental info

Sequence conflict4261I → M Ref.3
Sequence conflict8131D → A Ref.3
Sequence conflict4376 – 43772LN → FK Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 264040B0DDCDF54E

FASTA4,383491,305
        10         20         30         40         50         60 
MSKINKYGLE LHWAPEFPWM FEDAEEKLDN PSSSEVDMIC STTAQKLETD GICPENHVMV 

        70         80         90        100        110        120 
DCRRLLKQEC CVQSSLIREI VMNASPYHLE VLLQDALQSR EAVLVTTPLG MSLEACYVRG 

       130        140        150        160        170        180 
CNPKGWTMGL FRRRSVCNTG RCTVNKHVAY QLYMIDPAGV CLGAGQFVGW VIPLAFMPVQ 

       190        200        210        220        230        240 
SRKFIVPWVM YLRKRGEKGA YNKDHGCGGF GHVYDFKVED AYDQVHDEPK GKFSKKAYAL 

       250        260        270        280        290        300 
IRGYRGVKPL LYVDQYGCDY TGSLADGLEA YADKTLQEMK ALFPTWSQEL PFDVIVAWHV 

       310        320        330        340        350        360 
VRDPRYVMRL QSAATICSVA YVANPTEDLC DGSVVIKEPV HVYADDSIIL RQYNLFDIMS 

       370        380        390        400        410        420 
HFYMEADTVV NAFYGVALKD CGFVMQFGYI DCEQDSCDFK GWIPGNMIDG FACTTCGHVY 

       430        440        450        460        470        480 
EVGDLIAQSS GVLPVNPVLH TKSAAGYGGF GCKDSFTLYG QTVVYFGGCV YWSPARNIWI 

       490        500        510        520        530        540 
PILKSSVKSY DSLVYTGVLG CKAIVKETNL ICKALYLDYV QHKCGNLHQR ELLGVSDVWH 

       550        560        570        580        590        600 
KQLLINRGVY KPLLENIDYF NMRRAKFSLE TFTVCADGFM PFLLDDLVPR AYYLAVSGQA 

       610        620        630        640        650        660 
FCDYADKLCH AVVSKSKELL DVSLDSLGAA IHYLNSKIVD LAQHFSDFGT SFVSKIVHFF 

       670        680        690        700        710        720 
KTFTTSTALA FAWVLFHVLH GAYIVVESDI YFVKNIPRYA SAVAQAFQSV AKVVLDSLRV 

       730        740        750        760        770        780 
TFIDGLSCFK IGRRRICLSG RKIYEVERGL LHSSQLPLDV YDLTMPSQVQ KAKQKPIYLK 

       790        800        810        820        830        840 
GSGSDFSLAD SVVEVVTTSL TPCGYSEPPK VADKICIVDN VYMAKAGDKY YPVVVDDHVG 

       850        860        870        880        890        900 
LLDQAWRVPC AGRRVTFKEQ PTVKEIISMP KIIKVFYELD NDFNTILNTA CGVFEVDDTV 

       910        920        930        940        950        960 
DMEEFYAVVI DAIEEKLSPC KELEGVGAKV SAFLQKLEDN PLFLFDEAGE EVFAPKLYCA 

       970        980        990       1000       1010       1020 
FTAPEDDDFL EESDVEEDDV EGEETDLTIT SAGQPCVASE QEESSEVLED TLDDGPSVET 

      1030       1040       1050       1060       1070       1080 
SDSQVEEDVE MSDFVDLESV IQDYENVCFE FYTTEPEFVK VLGLYVPKAT RNNCWLRSVL 

      1090       1100       1110       1120       1130       1140 
AVMQKLPCQF KDKNLQDLWV LYKQQYSQLF VDTLVNKIPA NIVLPQGGYV ADFAYWFLTL 

      1150       1160       1170       1180       1190       1200 
CDWQCVAYWK CIKCDLALKL KGLDAMFFYG DVVSHICKCG ESMVLIDVDV PFTAHFALKD 

      1210       1220       1230       1240       1250       1260 
KLFCAFITKR IVYKAACVVD VNDSHSMAVV DGKQIDDHRI TSITSDKFDF IIGHGMSFSM 

      1270       1280       1290       1300       1310       1320 
TTFEIAQLYG SCITPNVCFV KGDIIKVSKL VKAEVVVNPA NGHMVHGGGV AKAIAVAAGQ 

      1330       1340       1350       1360       1370       1380 
QFVKETTNMV KSKGVCATGD CYVSTGGKLC KTVLNVVGPD ARTQGKQSYV LLERVYKHFN 

      1390       1400       1410       1420       1430       1440 
NYDCVVTTLI SAGIFSVPSD VSLTYLLGTA KKQVVLVSNN QEDFDLISKC QITAVEGTKK 

      1450       1460       1470       1480       1490       1500 
LAARLSFNVG RSIVYETDAN KLILINDVAF VSTFNVLQDV LSLRHDIALD DDARTFVQSN 

      1510       1520       1530       1540       1550       1560 
VDVLPEGWRV VNKFYQINGV RTVKYFECTG GIDICSQDKV FGYVQQGIFN KATVAQIKAL 

      1570       1580       1590       1600       1610       1620 
FLDKVDILLT VDGVNFTNRF VPVGESFGKS LGNVFCDGVN VTKHKCDINY KGKVFFQFDN 

      1630       1640       1650       1660       1670       1680 
LSSEDLKAVR SSFNFDQKEL LAYYNMLVNC FKWQVVVNGK YFTFKQANNN CFVNVSCLML 

      1690       1700       1710       1720       1730       1740 
QSLHLTFKIV QWQEAWLEFR SGRPARFVAL VLAKGGFKFG DPADSRDFLR VVFSQVDLTG 

      1750       1760       1770       1780       1790       1800 
AICDFEIACK CGVKQEQRTG LDAVMHFGTL SREDLEIGYT VDCSCGKKLI HCVRFDVPFL 

      1810       1820       1830       1840       1850       1860 
ICSNTPASVK LPKGVGSANI FIGDNVGHYV HVKCEQSYQL YDASNVKKVT DVTGKLSDCL 

      1870       1880       1890       1900       1910       1920 
YLKNLKQTFK SVLTTYYLDD VKKIEYKPDL SQYYCDGGKY YTQRIIKAQF KTFEKVDGVY 

      1930       1940       1950       1960       1970       1980 
TNFKLIGHTV CDSLNSKLGF DSSKEFVEYK ITEWPTATGD VVLANDDLYV KRYERGCITF 

      1990       2000       2010       2020       2030       2040 
GKPVIWLSHE KASLNSLTYF NRPLLVDDNK FDVLKVDDVD DSGDSSESGA KETKEINIIK 

      2050       2060       2070       2080       2090       2100 
LSGVKKPFKV EDSVIVNDDT SETKYVKSLS IVDVYDMWLT GCKYVVRTAN ALSRAVNVPT 

      2110       2120       2130       2140       2150       2160 
IRKFIKFGMT LVSIPIDLLN LREIKPAVNV VKAVRNKTSA CFNFIKWLFV LLFGWIKISA 

      2170       2180       2190       2200       2210       2220 
DNKVIYTTEI ASKLTCKLVA LAFKNAFLTF KWSMVARGAC IIATIFLLWF NFIYANVIFS 

      2230       2240       2250       2260       2270       2280 
DFYLPKIGFL PTFVGKIAQW IKNTFSLVTI CDLYSIQDVG FKNQYCNGSI ACQFCLAGFD 

      2290       2300       2310       2320       2330       2340 
MLDNYKAIDV VQYEADRRAF VDYTGVLKIV IELIVSYALY TAWFYPLFAL ISIQILTTWL 

      2350       2360       2370       2380       2390       2400 
PELFMLSTLH WSFRLLVALA NMLPAHVFMR FYIIIASFIK LFSLFKHVAY GCSKSGCLFC 

      2410       2420       2430       2440       2450       2460 
YKRNRSLRVK CSTIVGGMIR YYDVMANGGT GFCSKHQWNC IDCDSYKPGN TFITVEAALD 

      2470       2480       2490       2500       2510       2520 
LSKELKRPIQ PTDVAYHTVT DVKQVGCSMR LFYDRDGQRI YDDVNASLFV DYSNLLHSKV 

      2530       2540       2550       2560       2570       2580 
KSVPNMHVVV VENDADKANF LNAAVFYAQS LFRPILMVDK NLITTANTGT SVTETMFDVY 

      2590       2600       2610       2620       2630       2640 
VDTFLSMFDV DKKSLNALIA TAHSSIKQGT QIYKVLDTFL SCARKSCSID SDVDTKCLAD 

      2650       2660       2670       2680       2690       2700 
SVMSAVSAGL ELTDESCNNL VPTYLKSDNI VAADLGVLIQ NSAKHVQGNV AKIAGVSCIW 

      2710       2720       2730       2740       2750       2760 
SVDAFNQFSS DFQHKLKKAC CKTGLKLKLT YNKQMANVSV LTTPFSLKGG AVFSYFVYVC 

      2770       2780       2790       2800       2810       2820 
FVLSLVCFIG LWCLMPTYTV HKSDFQLPVY ASYKVLDNGV IRDVSVEDVC FANKFEQFDQ 

      2830       2840       2850       2860       2870       2880 
WYESTFGLSY YSNSMACPIV VAVIDQDFGS TVFNVPTKVL RYGYHVLHFI THALSADGVQ 

      2890       2900       2910       2920       2930       2940 
CYTPHSQISY SNFYASGCVL SSACTMFTMA DGSPQPYCYT DGLMQNASLY SSLVPHVRYN 

      2950       2960       2970       2980       2990       3000 
LANAKGFIRF PEVLREGLVR VVRTRSMSYC RVGLCEEADE GICFNFNGSW VLNNDYYRSL 

      3010       3020       3030       3040       3050       3060 
PGTFCGRDVF DLIYQLFKGL AQPVDFLALT ASSIAGAILA VIVVLVFYYL IKLKRAFGDY 

      3070       3080       3090       3100       3110       3120 
TSVVFVNVIV WCVNFMMLFV FQVYPTLSCV YAICYFYATL YFPSEISVIM HLQWLVMYGT 

      3130       3140       3150       3160       3170       3180 
IMPLWFCLLY IAVVVSNHAF WVFSYCRKLG TSVRSDGTFE EMALTTFMIT KDSYCKLKNS 

      3190       3200       3210       3220       3230       3240 
LSDVAFNRYL SLYNKYRYYS GKMDTAAYRE AACSQLAKAM DTFTNNNGSD VLYQPPTASV 

      3250       3260       3270       3280       3290       3300 
STSFLQSGIV KMVNPTSKVE PCVVSVTYGN MTLNGLWLDD KVYCPRHVIC SASDMTNPDY 

      3310       3320       3330       3340       3350       3360 
TNLLCRVTSS DFTVLFDRLS LTVMSYQMRG CMLVLTVTLQ NSRTPKYTFG VVKPGETFTV 

      3370       3380       3390       3400       3410       3420 
LAAYNGKPQG AFHVTMRSSY TIKGSFLCGS CGSVGYVIMG DCVKFVYMHQ LELSTGCHTG 

      3430       3440       3450       3460       3470       3480 
TDFNGDFYGP YKDAQVVQLP IQDYIQSVNF LAWLYAAILN NCNWFIQSDK CSVEDFNVWA 

      3490       3500       3510       3520       3530       3540 
LSNGFSQVKS DLVIDALASM TGVSLETLLA AIKRLKNGFQ GRQIMGSCSF EDELTPSDVY 

      3550       3560       3570       3580       3590       3600 
QQLAGIKLQS KRTRLFKGTV CWIMASTFLF SCIITAFVKW TMFMYVTTNM FSITFCALCV 

      3610       3620       3630       3640       3650       3660 
ISLAMLLVKH KHLYLTMYIT PVLFTLLYNN YLVVYKHTFR GYVYAWLSYY VPSVEYTYTD 

      3670       3680       3690       3700       3710       3720 
EVIYGMLLLV GMVFVTLRSI NHDLFSFIMF VGRLISVFSL WYKGSNLEEE ILLMLASLFG 

      3730       3740       3750       3760       3770       3780 
TYTWTTVLSM AVAKVIAKWV AVNVLYFTDI PQIKIVLLCY LFIGYIISCY WGLFSLMNSL 

      3790       3800       3810       3820       3830       3840 
FRMPLGVYNY KISVQELRYM NANGLRPPKN SFEALMLNFK LLGIGGVPII EVSQFQSKLT 

      3850       3860       3870       3880       3890       3900 
DVKCANVVLL NCLQHLHVAS NSKLWHYCST LHNEILATSD LSVAFEKLAQ LLIVLFANPA 

      3910       3920       3930       3940       3950       3960 
AVDSKCLTSI EEVCDDYAKD NTVLQALQSE FVNMASFVEY EVAKKNLDEA RFSGSANQQQ 

      3970       3980       3990       4000       4010       4020 
LKQLEKACNI AKSAYERDRA VAKKLERMAD LALTNMYKEA RINDKKSKVV SALQTMLFSM 

      4030       4040       4050       4060       4070       4080 
VRKLDNQALN SILDNAVKGC VPLNAIPSLA ANTLNIIVPD KSVYDQIVDN IYVTYAGNVW 

      4090       4100       4110       4120       4130       4140 
QIQTIQDSDG TNKQLNEISD DCNWPLVIIA NRYNEVSATV LQNNELMPAK LKIQVVNSGP 

      4150       4160       4170       4180       4190       4200 
DQTCNTPTQC YYNNSNNGKI VYAILSDVDG LKYTKILKDD GNFVVLELDP PCKFTVQDAK 

      4210       4220       4230       4240       4250       4260 
GLKIKYLYFV KGCNTLARGW VVGTISSTVR LQAGTATEYA SNSSILSLCA FSVDPKKTYL 

      4270       4280       4290       4300       4310       4320 
DFIQQGGTPI ANCVKMLCDH AGTGMAITVK PDATTSQDSY GGASVCIYCR ARVEHPDVDG 

      4330       4340       4350       4360       4370       4380 
LCKLRGKFVQ VPVGIKDPVS YVLTHDVCRV CGFWRDGSCS CVSTDTTVQS KDTNFLNGFG 


VRV 

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Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

See P0C6X6.

References

[1]"Circulation of genetically distinct contemporary human coronavirus OC43 strains."
Vijgen L., Keyaerts E., Lemey P., Moes E., Li S., Vandamme A.M., Van Ranst M.
Virology 337:85-92(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate 19572 Belgium 2004 and Isolate 87309 Belgium 2003.
[2]"Human respiratory coronavirus OC43: genetic stability and neuroinvasion."
St Jean J.R., Jacomy H., Desforges M., Vabret A., Freymuth F., Talbot P.J.
J. Virol. 78:8824-8834(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.
[3]"Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event."
Vijgen L., Keyaerts E., Moes E., Thoelen I., Wollants E., Lemey P., Vandamme A.M., Van Ranst M.
J. Virol. 79:1595-1604(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate ATCC VR-759.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY903459 Genomic RNA. No translation available.
AY903460 Genomic RNA. No translation available.
AY585228 Genomic RNA. No translation available.
AY585229 Genomic RNA. No translation available.
AY391777 Genomic RNA. No translation available.

3D structure databases

ProteinModelPortalP0C6U7.
SMRP0C6U7. Positions 3964-4114, 4238-4362.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR022570. Coronavirus_NSP1.
IPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_sub.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF11963. DUF3477. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1A_CVHOC
AccessionPrimary (citable) accession number: P0C6U7
Secondary accession number(s): Q9WAC3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries