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P0C6U6 (R1A_CVHNL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1a

Short name=pp1a
Alternative name(s):
ORF1a polyprotein

Cleaved into the following 11 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    p9
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p87
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.-
    Alternative name(s):
    PL1-PRO/PL2-PRO
    PLP1/PLP2
    Papain-like proteinases 1/2
    p195
  4. Non-structural protein 4
    Short name=nsp4
    Alternative name(s):
    Peptide HD2
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    M-PRO
    nsp5
    p34
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
    Alternative name(s):
    p5
  8. Non-structural protein 8
    Short name=nsp8
    Alternative name(s):
    p23
  9. Non-structural protein 9
    Short name=nsp9
    Alternative name(s):
    p12
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
    p14
  11. Non-structural protein 11
    Short name=nsp11
Gene names
ORF Names:1a
OrganismHuman coronavirus NL63 (HCoV-NL63) [Complete proteome]
Taxonomic identifier277944 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeAlphacoronavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length4060 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3. Ref.2

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity. Ref.2

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity. Ref.2

Nsp9 is a ssRNA-binding protein By similarity. Ref.2

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 Macro domain.

Contains 2 peptidase C16 domains.

Contains 1 peptidase C30 domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host IRF3 by virus
Inhibition of host RLR pathway by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandMetal-binding
RNA-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processinduction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host IRF3 activity

Inferred from electronic annotation. Source: UniProtKB-KW

viral genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: InterPro

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1a (identifier: P0C6U6-1)

Also known as: pp1a; ORF1a polyprotein;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6X5-1)

Also known as: pp1ab;

The sequence of this isoform can be found in the external entry P0C6X5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 40604060Replicase polyprotein 1a
PRO_0000338230
Chain1 – 110110Non-structural protein 1 By similarity
PRO_0000338231
Chain111 – 898788Non-structural protein 2 By similarity
PRO_0000338232
Chain899 – 24621564Non-structural protein 3 By similarity
PRO_0000338233
Chain2463 – 2939477Non-structural protein 4 By similarity
PRO_0000338234
Chain2940 – 32423033C-like proteinase By similarity
PRO_0000338235
Chain3243 – 3521279Non-structural protein 6 By similarity
PRO_0000338236
Chain3522 – 360483Non-structural protein 7 By similarity
PRO_0000338237
Chain3605 – 3799195Non-structural protein 8 By similarity
PRO_0000338238
Chain3800 – 3908109Non-structural protein 9 By similarity
PRO_0000338239
Chain3909 – 4043135Non-structural protein 10 By similarity
PRO_0000338240
Chain4044 – 406017Non-structural protein 11 Potential
PRO_0000338241

Regions

Transmembrane1903 – 192321Helical; Potential
Transmembrane1968 – 198821Helical; Potential
Transmembrane2050 – 207021Helical; Potential
Transmembrane2073 – 209321Helical; Potential
Transmembrane2111 – 213121Helical; Potential
Transmembrane2468 – 248821Helical; Potential
Transmembrane2727 – 274721Helical; Potential
Transmembrane2752 – 276918Helical; Potential
Transmembrane2772 – 279221Helical; Potential
Transmembrane2800 – 282021Helical; Potential
Transmembrane3254 – 327421Helical; Potential
Transmembrane3279 – 329921Helical; Potential
Transmembrane3303 – 332321Helical; Potential
Transmembrane3342 – 336221Helical; Potential
Transmembrane3376 – 339621Helical; Potential
Transmembrane3397 – 341721Helical; Potential
Transmembrane3442 – 346221Helical; Potential
Domain1021 – 1262242Peptidase C16 1
Domain1263 – 1421159Macro
Domain1640 – 1886247Peptidase C16 2
Domain2940 – 3242303Peptidase C30
Zinc finger1134 – 116532C4-type 1 By similarity
Zinc finger1757 – 178832C4-type 2; atypical By similarity
Zinc finger3982 – 399817 By similarity
Zinc finger4024 – 403714 By similarity
Region1903 – 2131229HD1 By similarity
Region2468 – 2820353HD2 By similarity
Region3254 – 3462209HD3 By similarity
Compositional bias625 – 6284Poly-Leu
Compositional bias1975 – 19784Poly-Leu

Sites

Active site10621For PL1-PRO activity By similarity
Active site12121For PL1-PRO activity By similarity
Active site16781For PL2-PRO activity By similarity
Active site18361For PL2-PRO activity By similarity
Active site29801For 3CL-PRO activity By similarity
Active site30831For 3CL-PRO activity By similarity
Site110 – 1112Cleavage; by PL1-PRO By similarity
Site898 – 8992Cleavage; by PL1-PRO By similarity
Site2462 – 24632Cleavage; by PL2-PRO By similarity
Site2939 – 29402Cleavage; by 3CL-PRO By similarity
Site3242 – 32432Cleavage; by 3CL-PRO By similarity
Site3521 – 35222Cleavage; by 3CL-PRO By similarity
Site3604 – 36052Cleavage; by 3CL-PRO By similarity
Site3799 – 38002Cleavage; by 3CL-PRO By similarity
Site3908 – 39092Cleavage; by 3CL-PRO By similarity
Site4043 – 40442Cleavage; by 3CL-PRO By similarity

Secondary structure

................................................................................ 4060
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 0F6313C474B7ED9C

FASTA4,060451,388
        10         20         30         40         50         60 
MFYNQVTLAV ASDSEISGFG FAIPSVAVRT YSEAAAQGFQ ACRFVAFGLQ DCVTGINDDD 

        70         80         90        100        110        120 
YVIALTGTNQ LCAKILPFSD RPLNLRGWLI FSNSNYVLQD FDVVFGHGAG SVVFVDKYMC 

       130        140        150        160        170        180 
GFDGKPVLPK NMWEFRDYFN NNTDSIVIGG VTYQLAWDVI RKDLSYEQQN VLAIESIHYL 

       190        200        210        220        230        240 
GTTGHTLKSG CKLTNAKPPK YSSKVVLSGE WNAVYRAFGS PFITNGMSLL DIIVKPVFFN 

       250        260        270        280        290        300 
AFVKCNCGSE SWSVGAWDGY LSSCCGTPAK KLCVVPGNVV PGDVIITSTS AGCGVKYYAG 

       310        320        330        340        350        360 
LVVKHITNIT GVSLWRVTAV HSDGMFVASS SYDALLHRNS LDPFCFDVNT LLSNQLRLAF 

       370        380        390        400        410        420 
LGASVTEDVK FAASTGVIDI SAGMFGLYDD ILTNNKPWFV RKASGLFDAI WDAFVAAIKL 

       430        440        450        460        470        480 
VPTTTGVLVR FVKSIASTVL TVSNGVIIMC ADVPDAFQSV YRTFTQAICA AFDFSLDVFK 

       490        500        510        520        530        540 
IGDVKFKRLG DYVLTENALV RLTTEVVRGV RDARIKKAMF TKVVVGPTTE VKFSVIELAT 

       550        560        570        580        590        600 
VNLRLVDCAP VVCPKGKIVV IAGQAFFYSG GFYRFMVDPT TVLNDPVFTG DLFYTIKFSG 

       610        620        630        640        650        660 
FKLDGFNHQF VTASSATDAI IAVELLLLDF KTAVFVYTCV VDGCSVIVRR DATFATHVCF 

       670        680        690        700        710        720 
KDCYNVWEQF CIDNCGEPWF LTDYNAILQS NNPQCAIVQA SESKVLLERF LPKCPEILLS 

       730        740        750        760        770        780 
IDDGHLWNLF VEKFNFVTDW LKTLKLTLTS NGLLGNCAKR FRRVLVKLLD VYNGFLETVC 

       790        800        810        820        830        840 
SVAYTAGVCI KYYAVNVPYV VISGFVSRVI RRERCDMTFP CVSCVTFFYE FLDTCFGVSK 

       850        860        870        880        890        900 
PNAIDVEHLE LKETVFVEPK DGGQFFVSGD YLWYVVDDIY YPASCNGVLP VAFTKLAGGK 

       910        920        930        940        950        960 
ISFSDDVIVH DVEPTHKVKL IFEFEDDVVT SLCKKSFGKS IIYTGDWEGL HEVLTSAMNV 

       970        980        990       1000       1010       1020 
IGQHIKLPQF YIYDEEGGYD VSKPVMISQW PISNDSNGCV VEASTDFHQL ECIVDDSVRE 

      1030       1040       1050       1060       1070       1080 
EVDIIEQPFE EVEHVLSIKQ PFSFSFRDEL GVRVLDQSDN NCWISTTLVQ LQLTKLLDDS 

      1090       1100       1110       1120       1130       1140 
IEMQLFKVGK VDSIVQKCYE LSHLISGSLG DSGKLLSELL KEKYTCSITF EMSCDCGKKF 

      1150       1160       1170       1180       1190       1200 
DDQVGCLFWI MPYTKLFQKG ECCICHKMQT YKLVSMKGTG VFVQDPAPID IDAFPVKPIC 

      1210       1220       1230       1240       1250       1260 
SSVYLGVKGS GHYQTNLYSF NKAIDGFGVF DIKNSSVNTV CFVDVDFHSV EIEAGEVKPF 

      1270       1280       1290       1300       1310       1320 
AVYKNVKFYL GDISHLVNCV SFDFVVNAAN ENLLHGGGVA RAIDILTEGQ LQSLSKDYIS 

      1330       1340       1350       1360       1370       1380 
SNGPLKVGAG VMLECEKFNV FNVVGPRTGK HEHSLLVEAY NSILFENGIP LMPLLSCGIF 

      1390       1400       1410       1420       1430       1440 
GVRIENSLKA LFSCDINKPL QVFVYSSNEE QAVLKFLDGL DLTPVIDDVD VVKPFRVEGN 

      1450       1460       1470       1480       1490       1500 
FSFFDCGVNA LDGDIYLLFT NSILMLDKQG QLLDTKLNGI LQQAALDYLA TVKTVPAGNL 

      1510       1520       1530       1540       1550       1560 
VKLFVESCTI YMCVVPSIND LSFDKNLGRC VRKLNRLKTC VIANVPAIDV LKKLLSSLTL 

      1570       1580       1590       1600       1610       1620 
TVKFVVESNV MDVNDCFKND NVVLKITEDG INVKDVVVES SKSLGKQLGV VSDGVDSFEG 

      1630       1640       1650       1660       1670       1680 
VLPINTDTVL SVAPEVDWVA FYGFEKAALF ASLDVKPYGY PNDFVGGFRV LGTTDNNCWV 

      1690       1700       1710       1720       1730       1740 
NATCIILQYL KPTFKSKGLN VLWNKFVTGD VGPFVSFIYF ITMSSKGQKG DAEEALSKLS 

      1750       1760       1770       1780       1790       1800 
EYLISDSIVT LEQYSTCDIC KSTVVEVKSA IVCASVLKDG CDVGFCPHRH KLRSRVKFVN 

      1810       1820       1830       1840       1850       1860 
GRVVITNVGE PIISQPSKLL NGIAYTTFSG SFDNGHYVVY DAANNAVYDG ARLFSSDLST 

      1870       1880       1890       1900       1910       1920 
LAVTAIVVVG GCVTSNVPTI VSEKISVMDK LDTGAQKFFQ FGDFVMNNIV LFLTWLLSMF 

      1930       1940       1950       1960       1970       1980 
SLLRTSIMKH DIKVIAKAPK RTGVILTRSF KYNIRSALFV IKQKWCVIVT LFKFLLLLYA 

      1990       2000       2010       2020       2030       2040 
IYALVFMIVQ FSPFNSLLCG DIVSGYEKST FNKDIYCGNS MVCKMCLFSY QEFNDLDHTS 

      2050       2060       2070       2080       2090       2100 
LVWKHIRDPI LISLQPFVIL VILLIFGNMY LRFGLLYFVA QFISTFGSFL GFHQKQWFLH 

      2110       2120       2130       2140       2150       2160 
FVPFDVLCNE FLATFIVCKI VLFVRHIIVG CNNADCVACS KSARLKRVPL QTIINGMHKS 

      2170       2180       2190       2200       2210       2220 
FYVNANGGTC FCNKHNFFCV NCDSFGPGNT FINGDIAREL GNVVKTAVQP TAPAYVIIDK 

      2230       2240       2250       2260       2270       2280 
VDFVNGFYRL YSGDTFWRYD FDITESKYSC KEVLKNCNVL ENFIVYNNSG SNITQIKNAC 

      2290       2300       2310       2320       2330       2340 
VYFSQLLCEP IKLVNSELLS TLSVDFNGVL HKAYVDVLCN SFFKELTANM SMAECKATLG 

      2350       2360       2370       2380       2390       2400 
LTVSDDDFVS AVANAHRYDV LLSDLSFNNF FISYAKPEDK LSVYDIACCM RAGSKVVNHN 

      2410       2420       2430       2440       2450       2460 
VLIKESIPIV WGVKDFNTLS QEGKKYLVKT TKAKGLTFLL TFNDNQAITQ VPATSIVAKQ 

      2470       2480       2490       2500       2510       2520 
GAGFKRTYNF LWYVCLFVVA LFIGVSFIDY TTTVTSFHGY DFKYIENGQL KVFEAPLHCV 

      2530       2540       2550       2560       2570       2580 
RNVFDNFNQW HEAKFGVVTT NSDKCPIVVG VSERINVVPG VPTNVYLVGK TLVFTLQAAF 

      2590       2600       2610       2620       2630       2640 
GNTGVCYDFD GVTTSDKCIF NSACTRLEGL GGDNVYCYNT DLIEGSKPYS TLQPNAYYKY 

      2650       2660       2670       2680       2690       2700 
DAKNYVRFPE ILARGFGLRT IRTLATRYCR VGECRDSHKG VCFGFDKWYV NDGRVDDGYI 

      2710       2720       2730       2740       2750       2760 
CGDGLIDLLV NVLSIFSSSF SVVAMSGHML FNFLFAAFIT FLCFLVTKFK RVFGDLSYGV 

      2770       2780       2790       2800       2810       2820 
FTVVCATLIN NISYVVTQNL FFMLLYAILY FVFTRTVRYA WIWHIAYIVA YFLLIPWWLL 

      2830       2840       2850       2860       2870       2880 
TWFSFAAFLE LLPNVFKLKI STQLFEGDKF IGTFESAAAG TFVLDMRSYE RLINTISPEK 

      2890       2900       2910       2920       2930       2940 
LKNYAASYNK YKYYSGSASE ADYRCACYAH LAKAMLDYAK DHNDMLYSPP TISYNSTLQS 

      2950       2960       2970       2980       2990       3000 
GLKKMAQPSG CVERCVVRVC YGSTVLNGVW LGDTVTCPRH VIAPSTTVLI DYDHAYSTMR 

      3010       3020       3030       3040       3050       3060 
LHNFSVSHNG VFLGVVGVTM HGSVLRIKVS QSNVHTPKHV FKTLKPGDSF NILACYEGIA 

      3070       3080       3090       3100       3110       3120 
SGVFGVNLRT NFTIKGSFIN GACGSPGYNV RNDGTVEFCY LHQIELGSGA HVGSDFTGSV 

      3130       3140       3150       3160       3170       3180 
YGNFDDQPSL QVESANLMLS DNVVAFLYAA LLNGCRWWLC STRVNVDGFN EWAMANGYTS 

      3190       3200       3210       3220       3230       3240 
VSSVECYSIL AAKTGVSVEQ LLASIQHLHE GFGGKNILGY SSLCDEFTLA EVVKQMYGVN 

      3250       3260       3270       3280       3290       3300 
LQSGKVIFGL KTMFLFSVFF TMFWAELFIY TNTIWINPVI LTPIFCLLLF LSLVLTMFLK 

      3310       3320       3330       3340       3350       3360 
HKFLFLQVFL LPTVIATALY NCVLDYYIVK FLADHFNYNV SVLQMDVQGL VNVLVCLFVV 

      3370       3380       3390       3400       3410       3420 
FLHTWRFSKE RFTHWFTYVC SLIAVAYTYF YSGDFLSLLV MFLCAISSDW YIGAIVFRLS 

      3430       3440       3450       3460       3470       3480 
RLIVFFSPES VFSVFGDVKL TLVVYLICGY LVCTYWGILY WFNRFFKCTM GVYDFKVSAA 

      3490       3500       3510       3520       3530       3540 
EFKYMVANGL HAPHGPFDAL WLSFKLLGIG GDRCIKISTV QSKLTDLKCT NVVLLGCLSS 

      3550       3560       3570       3580       3590       3600 
MNIAANSSEW AYCVDLHNKI NLCDDPEKAQ SMLLALLAFF LSKHSDFGLD GLIDSYFDNS 

      3610       3620       3630       3640       3650       3660 
STLQSVASSF VSMPSYIAYE NARQAYEDAI ANGSSSQLIK QLKRAMNIAK SEFDHEISVQ 

      3670       3680       3690       3700       3710       3720 
KKINRMAEQA ATQMYKEARS VNRKSKVISA MHSLLFGMLR RLDMSSVETV LNLARDGVVP 

      3730       3740       3750       3760       3770       3780 
LSVIPATSAS KLTIVSPDLE SYSKIVCDGS VHYAGVVWTL NDVKDNDGRP VHVKEITKEN 

      3790       3800       3810       3820       3830       3840 
VETLTWPLIL NCERVVKLQN NEIMPGKLKQ KPMKAEGDGG VLGDGNALYN TEGGKTFMYA 

      3850       3860       3870       3880       3890       3900 
YISNKADLKF VKWEYEGGCN TIELDSPCRF MVETPNGPQV KYLYFVKNLN TLRRGAVLGF 

      3910       3920       3930       3940       3950       3960 
IGATIRLQAG KQTELAVNSG LLTACAFSVD PATTYLEAVK HGAKPVSNCI KMLSNGAGNG 

      3970       3980       3990       4000       4010       4020 
QAITTSVDAN TNQDSYGGAS ICLYCRAHVP HPSMDGYCKF KGKCVQVPIG CLDPIRFCLE 

      4030       4040       4050       4060 
NNVCNVCGCW LGHGCACDRT TIQSVDISYL NEQGVLVQLD 

« Hide

Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

See P0C6X5.

References

[1]"Identification of a new human coronavirus."
Van Der Hoek L., Pyrc K., Jebbink M.F., Vermeulen-Oost W., Berkhout R.J., Wolthers K.C., Wertheim-Van Dillen P.M., Kaandorp J., Spaargaren J., Berkhout B.
Nat. Med. 10:368-373(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate Amsterdam I.
[2]"Deubiquitinating and interferon antagonism activities of coronavirus papain-like proteases."
Clementz M.A., Chen Z., Banach B.S., Wang Y., Sun L., Ratia K., Baez-Santos Y.M., Wang J., Takayama J., Ghosh A.K., Li K., Mesecar A.D., Baker S.C.
J. Virol. 84:4619-4629(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY567487 Genomic RNA. No translation available.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2VRIX-ray1.80A1258-1421[»]
3TLOX-ray1.60A/B2940-3242[»]
ProteinModelPortalP0C6U6.
SMRP0C6U6. Positions 2940-3240, 3802-3907, 3915-4039.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002589. Macro_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR011050. Pectin_lyase_fold/virulence.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 2 hits.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF51126. SSF51126. 1 hit.
PROSITEPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0C6U6.

Entry information

Entry nameR1A_CVHNL
AccessionPrimary (citable) accession number: P0C6U6
Secondary accession number(s): Q6Q1S3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references