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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1161For PL1-PRO activityPROSITE-ProRule annotation1
Active sitei1312For PL1-PRO activityPROSITE-ProRule annotation1
Active sitei1757For PL2-PRO activityPROSITE-ProRule annotation1
Active sitei1914For PL2-PRO activityPROSITE-ProRule annotation1
Active sitei3375For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3479For 3CL-PRO activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1238 – 1266C4-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri1835 – 1871C4-type 2PROSITE-ProRule annotationAdd BLAST37
Zinc fingeri4394 – 4410By similarityAdd BLAST17
Zinc fingeri4436 – 4449By similarityAdd BLAST14

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
p28
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65
Alternative name(s):
PL1-PRO/PL2-PRO
PL1/PL2
Papain-like proteinases 1/2
p210
Non-structural protein 4
Short name:
nsp4
Alternative name(s):
Peptide HD2
p44
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
M-PRO
nsp5
p27
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Alternative name(s):
p10
Non-structural protein 8
Short name:
nsp8
Alternative name(s):
p22
Non-structural protein 9
Short name:
nsp9
Alternative name(s):
p12
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
p15
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiHuman coronavirus HKU1 (isolate N1) (HCoV-HKU1)
Taxonomic identifieri443239 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008170 Componenti: Genome

Subcellular locationi

Non-structural protein 7 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Non-structural protein 8 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Non-structural protein 9 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Non-structural protein 10 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2226 – 2246HelicalSequence analysisAdd BLAST21
Transmembranei2287 – 2307HelicalSequence analysisAdd BLAST21
Transmembranei2318 – 2338HelicalSequence analysisAdd BLAST21
Transmembranei2401 – 2421HelicalSequence analysisAdd BLAST21
Transmembranei2443 – 2463HelicalSequence analysisAdd BLAST21
Transmembranei2844 – 2864HelicalSequence analysisAdd BLAST21
Transmembranei3119 – 3139HelicalSequence analysisAdd BLAST21
Transmembranei3151 – 3171HelicalSequence analysisAdd BLAST21
Transmembranei3178 – 3198HelicalSequence analysisAdd BLAST21
Transmembranei3203 – 3223HelicalSequence analysisAdd BLAST21
Transmembranei3651 – 3671HelicalSequence analysisAdd BLAST21
Transmembranei3676 – 3696HelicalSequence analysisAdd BLAST21
Transmembranei3701 – 3721HelicalSequence analysisAdd BLAST21
Transmembranei3744 – 3764HelicalSequence analysisAdd BLAST21
Transmembranei3772 – 3792HelicalSequence analysisAdd BLAST21
Transmembranei3800 – 3820HelicalSequence analysisAdd BLAST21
Transmembranei3843 – 3863HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003381941 – 4471Replicase polyprotein 1aAdd BLAST4471
ChainiPRO_00003381951 – 222Non-structural protein 1By similarityAdd BLAST222
ChainiPRO_0000338196223 – 809Non-structural protein 2By similarityAdd BLAST587
ChainiPRO_0000338197810 – 2838Non-structural protein 3By similarityAdd BLAST2029
ChainiPRO_00003381982839 – 3334Non-structural protein 4By similarityAdd BLAST496
ChainiPRO_00003381993335 – 36373C-like proteinaseBy similarityAdd BLAST303
ChainiPRO_00003382003638 – 3924Non-structural protein 6By similarityAdd BLAST287
ChainiPRO_00003382013925 – 4016Non-structural protein 7By similarityAdd BLAST92
ChainiPRO_00003382024017 – 4210Non-structural protein 8By similarityAdd BLAST194
ChainiPRO_00003382034211 – 4320Non-structural protein 9By similarityAdd BLAST110
ChainiPRO_00003382044321 – 4457Non-structural protein 10By similarityAdd BLAST137
ChainiPRO_00003382054458 – 4471Non-structural protein 11Sequence analysisAdd BLAST14

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei222 – 223Cleavage; by PL1-PROBy similarity2
Sitei809 – 810Cleavage; by PL1-PROBy similarity2
Sitei2838 – 2839Cleavage; by PL2-PROBy similarity2
Sitei3334 – 3335Cleavage; by 3CL-PROBy similarity2
Sitei3637 – 3638Cleavage; by 3CL-PROBy similarity2
Sitei3924 – 3925Cleavage; by 3CL-PROBy similarity2
Sitei4016 – 4017Cleavage; by 3CL-PROBy similarity2
Sitei4210 – 4211Cleavage; by 3CL-PROBy similarity2
Sitei4320 – 4321Cleavage; by 3CL-PROBy similarity2
Sitei4457 – 4458Cleavage; by 3CL-PROBy similarity2

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

Secondary structure

14471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3345 – 3347Combined sources3
Helixi3348 – 3350Combined sources3
Beta strandi3351 – 3356Combined sources6
Beta strandi3359 – 3366Combined sources8
Beta strandi3369 – 3373Combined sources5
Helixi3374 – 3377Combined sources4
Beta strandi3380 – 3382Combined sources3
Helixi3388 – 3394Combined sources7
Helixi3397 – 3399Combined sources3
Beta strandi3400 – 3404Combined sources5
Beta strandi3407 – 3409Combined sources3
Beta strandi3411 – 3417Combined sources7
Beta strandi3420 – 3427Combined sources8
Beta strandi3434 – 3437Combined sources4
Beta strandi3445 – 3452Combined sources8
Beta strandi3455 – 3463Combined sources9
Beta strandi3482 – 3485Combined sources4
Beta strandi3491 – 3502Combined sources12
Beta strandi3505 – 3509Combined sources5
Beta strandi3521 – 3524Combined sources4
Helixi3535 – 3547Combined sources13
Helixi3561 – 3571Combined sources11
Helixi3580 – 3589Combined sources10
Helixi3593 – 3604Combined sources12
Beta strandi3615 – 3617Combined sources3
Helixi3624 – 3632Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D23X-ray2.50A/B/C/D3335-3634[»]
ProteinModelPortaliP0C6U3.
SMRiP0C6U3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C6U3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati945 – 954110
Repeati955 – 964210
Repeati965 – 974310
Repeati975 – 984410
Repeati985 – 994510
Repeati995 – 1004610
Repeati1005 – 1014710
Repeati1015 – 1024810
Repeati1025 – 1034910
Repeati1035 – 10441010
Repeati1045 – 10541110
Repeati1055 – 10641210
Repeati1065 – 10741310
Repeati1075 – 10841410
Domaini1123 – 1373Peptidase C16 1PROSITE-ProRule annotationAdd BLAST251
Domaini1351 – 1522MacroPROSITE-ProRule annotationAdd BLAST172
Domaini1718 – 1978Peptidase C16 2PROSITE-ProRule annotationAdd BLAST261
Domaini3335 – 3637Peptidase C30PROSITE-ProRule annotationAdd BLAST303

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni945 – 108414 X 10 AA tandem repeat of N-[DN]-D-E-D-V-V-T-G-DAdd BLAST140
Regioni2226 – 2463HD1By similarityAdd BLAST238
Regioni2844 – 3223HD2By similarityAdd BLAST380
Regioni3651 – 3863HD3By similarityAdd BLAST213

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi934 – 1116Asp-richAdd BLAST183
Compositional biasi2229 – 2321Phe-richAdd BLAST93
Compositional biasi2616 – 2619Poly-Val4

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 2 peptidase C16 domains.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1238 – 1266C4-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri1835 – 1871C4-type 2PROSITE-ProRule annotationAdd BLAST37
Zinc fingeri4394 – 4410By similarityAdd BLAST17
Zinc fingeri4436 – 4449By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR022570. B-CoV_NSP1.
IPR032505. Corona_NSP4_C.
IPR002589. Macro_dom.
IPR032592. NAR_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_B_coronavir.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF11963. DUF3477. 1 hit.
PF01661. Macro. 1 hit.
PF16251. NAR. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Replicase polyprotein 1a (identifier: P0C6U3-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIKTSKYGLG FKWAPEFRWL LPDAAEELAS PMKSDEGGLC PSTGQAMESV
60 70 80 90 100
GFVYDNHVKI DCRCILGQEW HVQSNLIRDI FVHEDLHVVE VLTKTAVKSG
110 120 130 140 150
TAILIKSPLH SLGGFPKGYV MGLFRSYKTK RYVVHHLSMT TSTTNFGEDF
160 170 180 190 200
LGWIVPFGFM PSYVHKWFQF CRLYIEESDL IISNFKFDDY DFSVEDAYAE
210 220 230 240 250
VHAEPKGKYS QKAYALLRQY RGIKPVLFVD QYGCDYSGKL ADCLQAYGHY
260 270 280 290 300
SLQDMRQKQS VWLANCDFDI VVAWHVVRDS RFVMRLQTIA TICGIKYVAQ
310 320 330 340 350
PTEDVVDGDV VIREPVHLLS ADAIVLKLPS LMKVMTHMDD FSIKSIYNVD
360 370 380 390 400
LCDCGFVMQY GYVDCFNDNC DFYGWVSGNM MDGFSCPLCC TVYDSSEVKA
410 420 430 440 450
QSSGVIPENP VLFTNSTDTV NHDSFNLYGY SVTPFGSCIY WSPRPGLWIP
460 470 480 490 500
IIKSSVKSYD DLVYSGVVGC KSIVKETALI THALYLDYVQ CKCGNLEQNH
510 520 530 540 550
ILGVNNSWCR QLLLNRGDYN MLLKNIDLFV KRRADFACKF AVCGDGFVPF
560 570 580 590 600
LLDGLIPRSY YLIQSGIFFT SLMSQFSQEV SDMCLKMCIL FMDRVSVATF
610 620 630 640 650
YIEHYVNRLV TQFKLLGTTL VNKMVNWFNT MLDASAPATG WLLYQLLNGL
660 670 680 690 700
FVVSQANFNF VALIPDYAKI LVNKFYTFFK LLLECVTVDV LKDMPVLKTI
710 720 730 740 750
NGLVCIVGNK FYNVSTGLIP GFVLPCNAQE QQIYFFEGVA ESVIVEDDVI
760 770 780 790 800
ENVKSSLSSY EYCQPPKSVE KICIIDNMYM GKCGDKFFPI VMNDKNICLL
810 820 830 840 850
DQAWRFPCAG RKVNFNEKPV VMEIPSLMTV KVMFDLDSTF DDILGKVCSE
860 870 880 890 900
FEVEKGVTVD DFVAVVCDAI ENALNSCKEH PVVGYQVRAF LNKLNENVVY
910 920 930 940 950
LFDEAGDEAM ASRMYCTFAI EDVEDVISSE AVEDTIDGVV EDTINDDEDV
960 970 980 990 1000
VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV
1010 1020 1030 1040 1050
VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV
1060 1070 1080 1090 1100
VTGDNDDEDV VTGDNDDEDV VTGDNDDEDV VTGDNNDEEI VTGDNDDQIV
1110 1120 1130 1140 1150
VTGDDVDDIE SIYDFDTYKA LLVFNDVYND ALFVSYGSSV ETETYFKVNG
1160 1170 1180 1190 1200
LWSPTITHTN CWLRSVLLVM QKLPFKFKDL AIENMWLSYK VGYNQSFVDY
1210 1220 1230 1240 1250
LLTTIPKAIV LPQGGFVADF AYWFLNQFDI NAYANWCCLK CGFSFDLNGL
1260 1270 1280 1290 1300
DALFFYGDIV SHVCKCGHNM TLIAADLPCT LHFSLFDDNF CAFCTPKKIF
1310 1320 1330 1340 1350
IAACAVDVNV CHSVAVIGDE QIDGKFVTKF SGDKFDFIVG YGMSFSMSSF
1360 1370 1380 1390 1400
ELPQLYGLCI TPNVCFVKGD IINVARLVKA DVIVNPANGH MLHGGGVAKA
1410 1420 1430 1440 1450
IAVAAGKKFS KETAAMVKSK GVCQVGDCYV STGGKLCKTI LNIVGPDARQ
1460 1470 1480 1490 1500
DGRQSYVLLA RAYKHLNNYD CCLSTLISAG IFSVPADVSL TYLLGVVDKQ
1510 1520 1530 1540 1550
VILVSNNKED FDIIQKCQIT SVVGTKALAV RLTANVGRVI KFETDAYKLF
1560 1570 1580 1590 1600
LSGDDCFVSN SSVIQEVLLL RHDIQLNNDV RDYLLSKMTS LPKDWRLINK
1610 1620 1630 1640 1650
FDVINGVKTV KYFECPNSIY ICSQGKDFGY VCDGSFYKAT VNQVCVLLAK
1660 1670 1680 1690 1700
KIDVLLTVDG VNFKSISLTV GEVFGKILGN VFCDGIDVTK LKCSDFYADK
1710 1720 1730 1740 1750
ILYQYENLSL ADISAVQSSF GFDQQQLLAY YNFLTVCKWS VVVNGPFFSF
1760 1770 1780 1790 1800
EQSHNNCYVN VACLMLQHIN LKFNKWQWQE AWYEFRAGRP HRLVALVLAK
1810 1820 1830 1840 1850
GHFKFDEPSD ATDFIRVVLK QADLSGAICE LELICDCGIK QESRVGVDAV
1860 1870 1880 1890 1900
MHFGTLAKTD LFNGYKIGCN CAGRIVHCTK LNVPFLICSN TPLSKDLPDD
1910 1920 1930 1940 1950
VVAANMFMGV GVGHYTHLKC GSPYQHYDAC SVKKYTGVSG CLTDCLYLKN
1960 1970 1980 1990 2000
LTQTFTSMLT NYFLDDVEMV AYNPDLSQYY CDNGKYYTKP IIKAQFKPFA
2010 2020 2030 2040 2050
KVDGVYTNFK LVGHDICAQL NDKLGFNVDL PFVEYKVTVW PVATGDVVLA
2060 2070 2080 2090 2100
SDDLYVKRYF KGCETFGKPV IWFCHDEASL NSLTYFNKPS FKSENRYSVL
2110 2120 2130 2140 2150
SVDSVSEESQ GNVVTSVMES QISTKEVKLK GVRKTVKIED AIIVNDENSS
2160 2170 2180 2190 2200
IKVVKSLSLV DVWDMYLTGC DYVVWVANEL SRLVKSPTVR EYIRYGIKPI
2210 2220 2230 2240 2250
TIPIDLLCLR DDNQTLLVPK IFKARAIEFY GFLKWLFIYV FSLLHFTNDK
2260 2270 2280 2290 2300
TIFYTTEIAS KFTFNLFCLA LKNAFQTFRW SIFIKGFLVV ATVFLFWFNF
2310 2320 2330 2340 2350
LYINVIFSDF YLPNISVFPI FVGRIVMWIK ATFGLVTICD FYSKLGVGFT
2360 2370 2380 2390 2400
SHFCNGSFIC ELCHSGFDML DTYAAIDFVQ YEVDRRVLFD YVSLVKLIVE
2410 2420 2430 2440 2450
LVIGYSLYTV WFYPLFCLIG LQLFTTWLPD LFMLETMHWL IRFIVFVANM
2460 2470 2480 2490 2500
LPAFVLLRFY IVVTAMYKVV GFIRHIVYGC NKAGCLFCYK RNCSVRVKCS
2510 2520 2530 2540 2550
TIVGGVIRYY DITANGGTGF CVKHQWNCFN CHSFKPGNTF ITVEAAIELS
2560 2570 2580 2590 2600
KELKRPVNPT DASHYVVTDI KQVGCMMRLF YDRDGQRVYD DVDASLFVDI
2610 2620 2630 2640 2650
NNLLHSKVKV VPNLYVVVVE SDADRANFLN AVVFYAQSLY RPILLVDKKL
2660 2670 2680 2690 2700
ITTACNGISV TQTMFDVYVD TFMSHFDVDR KSFNNFVNIA HASLREGVQL
2710 2720 2730 2740 2750
EKVLDTFVGC VRKCCSIDSD VETRFITKSM ISAVAAGLEF TDENYNNLVP
2760 2770 2780 2790 2800
TYLKSDNIVA ADLGVLIQNG AKHVQGNVAK AANISCIWFI DAFNQLTADL
2810 2820 2830 2840 2850
QHKLKKACVK TGLKLKLTFN KQEASVPILT TPFSLKGGVV LSNLLYILFF
2860 2870 2880 2890 2900
VSLICFILLW ALLPTYSVYK SDIHLPAYAS FKVIDNGVVR DISVNDLCFA
2910 2920 2930 2940 2950
NKFFQFDQWY ESTFGSVYYH NSMDCPIVVA VMDEDIGSTM FNVPTKVLRH
2960 2970 2980 2990 3000
GFHVLHFLTY AFASDSVQCY TPHIQISYND FYASGCVLSS LCTMFKRGDG
3010 3020 3030 3040 3050
TPHPYCYSDG VMKNASLYTS LVPHTRYSLA NSNGFIRFPD VISEGIVRIV
3060 3070 3080 3090 3100
RTRSMTYCRV GACEYAEEGI CFNFNSSWVL NNDYYRSMPG TFCGRDLFDL
3110 3120 3130 3140 3150
FYQFFSSLIR PIDFFSLTAS SIFGAILAIV VVLVFYYLIK LKRAFGDYTS
3160 3170 3180 3190 3200
VVVINVVVWC INFLMLFVFQ VYPICACVYA CFYFYVTLYF PSEISVIMHL
3210 3220 3230 3240 3250
QWIVMYGAIM PFWFCVTYVA MVIANHVLWL FSYCRKIGVN VCSDSTFEET
3260 3270 3280 3290 3300
SLTTFMITKD SYCRLKNSVS DVAYNRYLSL YNKYRYYSGK MDTAAYREAA
3310 3320 3330 3340 3350
CSQLAKAMET FNHNNGNDVL YQPPTASVST SFLQSGIVKM VSPTSKIEPC
3360 3370 3380 3390 3400
IVSVTYGSMT LNGLWLDDKV YCPRHVICSS SNMNEPDYSA LLCRVTLGDF
3410 3420 3430 3440 3450
TIMSGRMSLT VVSYQMQGCQ LVLTVSLQNP YTPKYTFGNV KPGETFTVLA
3460 3470 3480 3490 3500
AYNGRPQGAF HVTMRSSYTI KGSFLCGSCG SVGYVLTGDS VKFVYMHQLE
3510 3520 3530 3540 3550
LSTGCHTGTD FTGNFYGPYR DAQVVQLPVK DYVQTVNVIA WLYAAILNNC
3560 3570 3580 3590 3600
AWFVQNDVCS TEDFNVWAMA NGFSQVKADL VLDALASMTG VSIETLLAAI
3610 3620 3630 3640 3650
KRLYMGFQGR QILGSCTFED ELAPSDVYQQ LAGVKLQSKT KRFIKETIYW
3660 3670 3680 3690 3700
ILISTFLFSC IISAFVKWTI FMYINTHMIG VTLCVLCFVS FMMLLVKHKH
3710 3720 3730 3740 3750
FYLTMYIIPV LCTLFYVNYL VVYKEGFRGF TYVWLSYFVP AVNFTYVYEV
3760 3770 3780 3790 3800
FYGCILCVFA IFITMHSINH DIFSLMFLVG RIVTLISMWY FGSNLEEDVL
3810 3820 3830 3840 3850
LFITAFLGTY TWTTILSLAI AKIVANWLSV NIFYFTDVPY IKLILLSYLF
3860 3870 3880 3890 3900
IGYILSCYWG FFSLLNSVFR MPMGVYNYKI SVQELRYMNA NGLRPPRNSF
3910 3920 3930 3940 3950
EAILLNLKLL GIGGVPVIEV SQIQSKLTDV KCANVVLLNC LQHLHVASNS
3960 3970 3980 3990 4000
KLWQYCSVLH NEILSTSDLS VAFDKLAQLL IVLFANPAAV DTKCLASIDE
4010 4020 4030 4040 4050
VSDDYVQDST VLQALQSEFV NMASFVEYEV AKKNLADAKN SGSVNQQQIK
4060 4070 4080 4090 4100
QLEKACNIAK SVYERDKAVA RKLERMADLA LTNMYKEARI NDKKSKVVSA
4110 4120 4130 4140 4150
LQTMLFSMVR KLDNQALNSI LDNAVKGCVP LSAIPALAAN TLTIVIPDKQ
4160 4170 4180 4190 4200
VFDKVVDNVY VTYAGSVWHI QTVQDADGIN KQLTDISVDS NWPLVIIANR
4210 4220 4230 4240 4250
YNEVANAVMQ NNELMPHKLK IQVVNSGSDM NCNIPTQCYY NNGSSGRIVY
4260 4270 4280 4290 4300
AVLSDVDGLK YTKIMKDDGN CVVLELDPPC KFSIQDVKGL KIKYLYFIKG
4310 4320 4330 4340 4350
CNTLARGWVV GTLSSTIRLQ AGVATEYAAN SSILSLCAFS VDPKKTYLDY
4360 4370 4380 4390 4400
IQQGGVPIIN CVKMLCDHAG TGMAITIKPE ATINQDSYGG ASVCIYCRAR
4410 4420 4430 4440 4450
VEHPDVDGIC KLRGKFVQVP LGIKDPILYV LTHDVCQVCG FWRDGSCSCV
4460 4470
GSSVAVQSKD LNFLNGFGVL V
Note: Produced by conventional translation.
Length:4,471
Mass (Da):502,589
Last modified:June 10, 2008 - v1
Checksum:i6A17587F5DF95C46
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6X2-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6X2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Length:7,182
Mass (Da):810,941
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY597011 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY597011 Genomic RNA. No translation available.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D23X-ray2.50A/B/C/D3335-3634[»]
ProteinModelPortaliP0C6U3.
SMRiP0C6U3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP0C6U3.

Family and domain databases

InterProiIPR022570. B-CoV_NSP1.
IPR032505. Corona_NSP4_C.
IPR002589. Macro_dom.
IPR032592. NAR_dom.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR002705. Pept_C30/C16_B_coronavir.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF11963. DUF3477. 1 hit.
PF01661. Macro. 1 hit.
PF16251. NAR. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF01831. Peptidase_C16. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiR1A_CVHN1
AccessioniPrimary (citable) accession number: P0C6U3
Secondary accession number(s): Q5MQD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolate N1 belongs to genotype A.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.