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P0C6T7

- R1A_BCRP3

UniProt

P0C6T7 - R1A_BCRP3

Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.By similarity
    The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.PROSITE-ProRule annotation
    Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
    Nsp9 is a ssRNA-binding protein.By similarity
    Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.By similarity

    Catalytic activityi

    TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei179 – 1802CleavageBy similarity
    Sitei818 – 8192Cleavage; by PL-PROBy similarity
    Active sitei1649 – 16491For PL-PRO activityPROSITE-ProRule annotation
    Active sitei1810 – 18101For PL-PRO activityPROSITE-ProRule annotation
    Sitei3238 – 32392Cleavage; by PL-PROBy similarity
    Active sitei3279 – 32791For 3CL-PRO activityPROSITE-ProRule annotation
    Active sitei3383 – 33831For 3CL-PRO activityPROSITE-ProRule annotation
    Sitei3544 – 35452Cleavage; by 3CL-PROBy similarity
    Sitei3834 – 38352Cleavage; by 3CL-PROBy similarity
    Sitei3917 – 39182Cleavage; by 3CL-PROBy similarity
    Sitei4115 – 41162Cleavage; by 3CL-PROBy similarity
    Sitei4228 – 42292Cleavage; by 3CL-PROBy similarity
    Sitei4367 – 43682Cleavage; by 3CL-PROBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1727 – 176438C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4302 – 431817By similarityAdd
    BLAST
    Zinc fingeri4345 – 435814By similarityAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: InterPro
    2. hydrolase activity, acting on acid anhydrides Source: InterPro
    3. hydrolase activity, acting on ester bonds Source: InterPro
    4. omega peptidase activity Source: InterPro
    5. RNA binding Source: UniProtKB-KW
    6. RNA-directed RNA polymerase activity Source: InterPro
    7. zinc ion binding Source: InterPro

    GO - Biological processi

    1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
    2. induction by virus of host autophagy Source: UniProtKB-KW
    3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
    4. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
    6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    7. viral genome replication Source: InterPro
    8. viral protein processing Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicase polyprotein 1a
    Short name:
    pp1a
    Alternative name(s):
    ORF1a polyprotein
    Cleaved into the following 11 chains:
    Non-structural protein 1
    Short name:
    nsp1
    Alternative name(s):
    Leader protein
    Non-structural protein 2
    Short name:
    nsp2
    Alternative name(s):
    p65 homolog
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name:
    PL-PRO
    Non-structural protein 4
    Short name:
    nsp4
    3C-like proteinase (EC:3.4.22.-)
    Short name:
    3CL-PRO
    Short name:
    3CLp
    Alternative name(s):
    nsp5
    Non-structural protein 6
    Short name:
    nsp6
    Non-structural protein 7
    Short name:
    nsp7
    Non-structural protein 8
    Short name:
    nsp8
    Non-structural protein 9
    Short name:
    nsp9
    Non-structural protein 10
    Short name:
    nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name:
    GFL
    Non-structural protein 11
    Short name:
    nsp11
    Gene namesi
    ORF Names:1a
    OrganismiBat coronavirus Rp3/2004 (BtCoV/Rp3/2004) (SARS-like coronavirus Rp3)
    Taxonomic identifieri349344 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiRhinolophus ferrumequinum (Greater horseshoe bat) [TaxID: 59479]
    Rhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
    Rhinolophus pearsonii [TaxID: 188571]
    Rhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399]
    ProteomesiUP000006570: Genome

    Subcellular locationi

    Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity
    Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
    Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.By similarity

    GO - Cellular componenti

    1. host cell membrane Source: UniProtKB-SubCell
    2. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 43804380Replicase polyprotein 1aPRO_0000338122Add
    BLAST
    Chaini1 – 179179Non-structural protein 1By similarityPRO_0000338123Add
    BLAST
    Chaini180 – 818639Non-structural protein 2By similarityPRO_0000338124Add
    BLAST
    Chaini819 – 27381920Non-structural protein 3By similarityPRO_0000338125Add
    BLAST
    Chaini2739 – 3238500Non-structural protein 4By similarityPRO_0000338126Add
    BLAST
    Chaini3239 – 35443063C-like proteinaseBy similarityPRO_0000338127Add
    BLAST
    Chaini3545 – 3834290Non-structural protein 6By similarityPRO_0000338128Add
    BLAST
    Chaini3835 – 391783Non-structural protein 7By similarityPRO_0000338129Add
    BLAST
    Chaini3918 – 4115198Non-structural protein 8By similarityPRO_0000338130Add
    BLAST
    Chaini4116 – 4228113Non-structural protein 9By similarityPRO_0000338131Add
    BLAST
    Chaini4229 – 4367139Non-structural protein 10By similarityPRO_0000338132Add
    BLAST
    Chaini4368 – 438013Non-structural protein 11Sequence AnalysisPRO_0000338133Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.By similarity

    Interactioni

    Subunit structurei

    3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0C6T7.
    SMRiP0C6T7. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2201 – 222121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2312 – 233423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2349 – 236921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei2753 – 277321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3020 – 304021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3059 – 307921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3081 – 310121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3103 – 312321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3140 – 316021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3562 – 358221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3584 – 360421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3610 – 363021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3657 – 367620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3683 – 370220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3726 – 374621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei3754 – 377421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1001 – 1167167MacroPROSITE-ProRule annotationAdd
    BLAST
    Domaini1609 – 1873265Peptidase C16PROSITE-ProRule annotationAdd
    BLAST
    Domaini3239 – 3544306Peptidase C30PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2201 – 2369169HD1By similarityAdd
    BLAST
    Regioni2753 – 3160408HD2By similarityAdd
    BLAST
    Regioni3562 – 3774213HD3By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi930 – 99970Glu-richAdd
    BLAST
    Compositional biasi2208 – 22114Poly-Leu
    Compositional biasi3764 – 37674Poly-Cys

    Domaini

    The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

    Sequence similaritiesi

    Contains 1 Macro domain.PROSITE-ProRule annotation
    Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1727 – 176438C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri4302 – 431817By similarityAdd
    BLAST
    Zinc fingeri4345 – 435814By similarityAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    InterProiIPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view]
    PfamiPF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view]
    SUPFAMiSSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    PROSITEiPS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform Replicase polyprotein 1a (identifier: P0C6T7-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1a, ORF1a polyprotein

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MESLVLGINE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKSGT     50
    CGIVELEKGV LPQPEQPYVF IKRSDAQGTD HGHRVRELVA ELDGVQYGRS 100
    GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG 150
    TDPIEDYEQN WNTKHGSGVL RELTRELNGG ALTRYVDNNF CGPDGYPLEC 200
    IKDLLARAGK SMCTLSEQLD YIESKRGVYC CRDHGHEIAW FTERSDKSYE 250
    HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI 300
    RSVYPVATPQ ECNNMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV 350
    SEGPNTCGYL PTNAVVKMPC PACQDPEIGP EHSAADYHNH SNIETRLRKG 400
    GRTRCFGGCV FAYVGCYNKR AYWVPRASAD IGSGHTGITG DNVETLNEDL 450
    LEILSRERVN INIVGDFQLN EEVAIILASF SASTSAFIDT IKSLDYKSFK 500
    TIVESCGNYK VTKGKPIKGA WNIGQHRSVL TPLCGFPSQA AGVIRSIFSR 550
    TLDAANHSIP DLQRAAVTIL DSISEQSLRL VDAMVYTSNL LTNSVIIMAY 600
    VTGGLVQQTS QWLSNLLDTT VEKLRPIFAW IEAKLSAGVE FLKDAWEILK 650
    FLITGVFDIV KGQIQVASDN IKDCVKCFVD VVNKALEMCI DQVTIAGAKL 700
    RSLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDSHDTV 750
    LTSEEVVLKN GELEALETPV DSFTNGAVVG TPVCINGLML LEIKANEQYC 800
    ALSPGLLATN NVFRLKGGAP TKGVTFGEDT VVEVQGYKNV RITFELDERV 850
    DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE 900
    WSVATFYLFD DSGEEKLSSR MYCSFYPPDE EEDCEEYEEE EEVSERTCEH 950
    EYGTEEDYKG LPLEFGASTD IIQVEEQEEE DWLDDAVEAE PEPEPLHEEP 1000
    VNQLTGYLKL TDNVAIKCVD IVEEAQNANP MVIVNAANIH LKHGGGVAGA 1050
    LNKATNGAMQ KESDHYIKLN GPLTVGGSCL LSGHNLAKKC LHVVGPNLNA 1100
    GEDIQLLKAA YENFNSQDIL LAPLLSAGIF GAKPLQSLQM CVQTVRTQVY 1150
    IVVNDKVLYE QVVMDYLDSL KPKVEAPKQE VLPKAEYPKV DEKSVVQKTI 1200
    DVKPKIKACI DEVTTTLEET KFLTNKLLLF TDINGKLYQD SKNMLRGEDM 1250
    SFLEKDAPYM VGDVITSGDI TCVVIPSKKA GGTTEMLSRA LKKVPINEYI 1300
    TTYPGQGCAG YTLEEAKTAL KKCKSAFYVL PSETPNAKEE ILGTVSWNLR 1350
    EMLAHAEETR KLMPVCMDVR AIMATIQRKY KGIKIQEGIV DYGVRFFFYT 1400
    SKEPVASIIT KLNSLNEPLV TMPIGYVTHG FNLEEAARCM RSLKAPAIVS 1450
    VSSPDAVTTY NGYLTSSSKT SEDHFVETVS LAGSYRDWSY SGQRTELGVE 1500
    FLKRGEKIVY HTLESPVKFH LDGEVLPLDK LKSLLSLREV KTIKVFTTVD 1550
    NTNLHTQLVD MSMTYGQQLG PTYLEGADVT KIKPHVNHEG KTFFVLPSDD 1600
    TLRSEAFEYY HTLDESFLGR YMSALNHTKK WKFPQVGGLT SIKWADNNCY 1650
    LSSVLLALQQ IEVKFNAPAL QEAYYRARAG DAANFCALIL AYSNKTVGEL 1700
    GDVRETMTHL LQHANLESAK RVLNVVCKHC GQKTTTLTGV EAVMYMGTLS 1750
    YDNLKMGVSI PCVCGRDATQ YLVQQESSFV MMSAPPAEYK LQQGTFLCAN 1800
    EYTGNYQCGH YTHITAKETL YRIDGAHLTK MSEYKGPVTD VFYKETSYTT 1850
    TIKPVSYKLD GVTYTEIEPK LDGYYKKDNA YYTEQPIDLI PTQPLPNASF 1900
    DNFKLTCSNT KFADDLNQMT GFTKPASREL SVTFFPDLNG DVVAIDYRHY 1950
    SASFKKGAKL LHKPIVWHIN QATTKTTFKP NTWCLRCLWS TKPVDTSNSF 2000
    EVLAVEDTQG MDNLACESQQ PTPEEVVENP TIQKEVIECD VKTTEVVGNV 2050
    ILKPSDEGVK VTQELDHEDL MAAYVENTSI TIKKPNELSL ALGLKTIATH 2100
    GIAAINSVPW GKILAYVKPF LGQAAVTTSN CAKRLVQRMF NNYMPYVLTL 2150
    LFQLCTFTKS TNSRIRASLP TTIAKNSVRG IVRLCLDAGI NYVKSPKFSK 2200
    LFTIAMWLLL LSICLGSLIY VTAALGVLLS NFGAPSYCSG VRESYLNSSN 2250
    VTTMDFCEGS FPCSVCLSGL DSLDSYPALE TIQVTISSYK LDLTILGLAA 2300
    EWFFAYMLFT KFFYLLGLSA IMQVFFGYFA SHFISNSWLM WFIISIVQMA 2350
    PVSAMVRMYI FFASFYYIWK SYVHIMDGCT SSTCMMCYKR NRATRVECTT 2400
    IVNGMKRSFY VYANGGRGFC KTHNWNCLNC DTFCAGSTFI SDEVARDLSL 2450
    QFKRPINPTD QSSYVVDSVA VKNGALHLYF DKAGQKTYER HPLSHFVNLD 2500
    NLRANNTKGS LPINVIVFDG KSKCDESAAK SASVYYSQLM CQPILLLDQA 2550
    LVSDVGDSTE VSVKMFDAYV DTFSATFSVP MEKLKALVAT AHSELAKGVA 2600
    LDGVLSTFVS ASRQGVVDTD VDTKDVIECL KLSHHSDLEV TGDSCNNFML 2650
    TYNKVENMTP RDLGACIDCN ARHINAQVAR SHNVSLIWNV KDYMSLSEQL 2700
    RKQIRSAAKK NNIPFRLTCA TTRQVVNVIT TKISLKGGKI VSTWFKIMLK 2750
    ATLLCVLAAL VCYIVMPVHI LSVHGGYTNE IIGYKAIQDG VTRDIVSTDD 2800
    CFANKHAGFD SWFSQRGGSY KNDKSCPVVA AIITREIGFI VPGLPGTVLR 2850
    AINGDFLHFL PRVFSAVGNI CYTPSKLIEY SDFSTSACVL AAECTIFKDA 2900
    MGKPVPYCYD TNLLEGSISY SELRPDTRYV LMDGSIIQFP NAYLEGSVRV 2950
    VTTFDAEYCR HGTCERSEAG ICLSTSGRWV LNNEHYRALP GVFCGVDAMN 3000
    LIANIFTPLV QPVGALDVSA SVVAGGIIAI LVTCAAYYFM KFRRAFGEYN 3050
    HVVAANAPLF LMSFTILCLA PAYSFLPGVY SVFYLYLTFY FTNDVSFLAH 3100
    LQWFAMFSPI VPFWITAIYV FCISLKHFHW FFNNYLRKRV VFNGVTFSTF 3150
    EEAALCTFLL NKEMYLKLRS ETLLPLTQYN RYLALYNKYK YFSGALDTTS 3200
    YREAACCHLA KALNDFSNSG ADVLYQPPQT SITSAVLQSG FRKMAFPSGK 3250
    VEGCMVQVTC GTTTLNGLWL DDTVYCPRHV ICTAEDMLNP NYEDLLIRKS 3300
    NHSFLVQAGN VQLRVIGHSM QNCLLRLKVD TSNPKTPKYK FVRIQPGQTF 3350
    SVLACYNGSP SGVYQCAMRP NHTIKGSFLN GSCGSVGFNI DYDCVSFCYM 3400
    HHMELPTEVH AGTDLEGKFY GPFVDRQTAQ AAGTDTTITL NVLAWLYAAV 3450
    INGDRWFLNR FTTTLNDFNL VAMKYNYEPL TQDHVDILGP LSAQTGIAVL 3500
    DMCAALKELL QNGMNGRTIL GSTILEDEFT PFDVVRQCSG VTFQGKFKRI 3550
    VKGTHHWMLL TFLTSLLILV QSTQWSLFFF VYENAFLPFT LGIMAVAACA 3600
    MLLVKHKHAF LCLFLLPSLA TVAYFNMVYM PASWVMRIMT WLELADTSLS 3650
    GYRLKDCVMY ASALVLLVLM TARTVYDDAA RRVWTLMNVI TLVYKVYYGN 3700
    ALDQAISMWA LVISVTSNYS GVVTTIMFLA RAIVFVCVEY YPLLFITGNT 3750
    LQCIMLVYCF LGYCCCCYFG LFCLLNRYFR LTLGVYDYLV STQEFRYMNS 3800
    QGLLPPKSSI DAFKLNIKLL GIGGKPCIKV ATVQSKMSDV KCTSVVLLSV 3850
    LQQLRVESSS KLWAQCVQLH NDILLAKDTT EAFEKMVSLL SVLLSMQGAV 3900
    DINKLCEEML DNRATLQAIA SEFSSLPSYA AYATAQEAYE QAVANGDSEV 3950
    VLKKLKKSLN VAKSEFDRNA AMQRKLEKMA DQAMTQMYKQ ARSEDKRAKV 4000
    TSAMQTMLFT MLRKLDNDAL NNIINNARDG CVPLNIIPLT TAAKLMVVVP 4050
    DYGTYKNTCD GNTFTYASAL WEIQQVVDAD SKIVQLSEIN MENSSNLAWP 4100
    LIVTALRANS AVKLQNNELS PVALRQMSCA AGTTQTACTD DNALAYYNNS 4150
    KGGRFVLALL SDHQDLKWAR FPKSDGTGTI YTELEPPCRF VTDTPKGPKV 4200
    KYLHFIKGLN NLNRGMVLGS LAATVRLQAG NATEVPANST VLSFCAFAVD 4250
    PAKAYKDYLA SGGQPITNCV KMLCTHTGTG QAITVTPEAN MDQESFGGAS 4300
    CCLYCRCHID HPNPKGFCDL KGKYVQIPTT CANDPVGFTL RNTVCTVCGM 4350
    WKGYGCSCDQ LREPMMQSAD ASTFLNGFAV 4380

    Note: Produced by conventional translation.

    Length:4,380
    Mass (Da):486,337
    Last modified:June 10, 2008 - v1
    Checksum:i0DDA57CD3DF1535B
    GO
    Isoform Replicase polyprotein 1ab (identifier: P0C6W6-1) [UniParc]FASTAAdd to Basket

    Also known as: pp1ab

    The sequence of this isoform can be found in the external entry P0C6W6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

    Length:7,071
    Mass (Da):790,103
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ071615 Genomic RNA. Translation: AAZ67050.1.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ071615 Genomic RNA. Translation: AAZ67050.1 .

    3D structure databases

    ProteinModelPortali P0C6T7.
    SMRi P0C6T7. Positions 13-127, 819-930, 997-1174, 1329-1467, 1539-1852, 3239-3544, 3834-3917, 3919-4109, 4116-4228, 4238-4360.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002589. Macro_dom.
    IPR021590. NSP1.
    IPR024375. Nsp3_coronavir.
    IPR014828. NSP7.
    IPR014829. NSP8.
    IPR014822. NSP9.
    IPR008740. Peptidase_C30.
    IPR013016. Peptidase_C30/C16.
    IPR018995. RNA_synth_NSP10_coronavirus.
    IPR024358. SARS-CoV_Nsp3_N.
    IPR022733. SARS_polyprot_cleavage.
    IPR009003. Trypsin-like_Pept_dom.
    IPR014827. Viral_protease.
    [Graphical view ]
    Pfami PF12379. DUF3655. 1 hit.
    PF01661. Macro. 1 hit.
    PF11501. Nsp1. 1 hit.
    PF09401. NSP10. 1 hit.
    PF12124. Nsp3_PL2pro. 1 hit.
    PF08716. nsp7. 1 hit.
    PF08717. nsp8. 1 hit.
    PF08710. nsp9. 1 hit.
    PF05409. Peptidase_C30. 1 hit.
    PF11633. SUD-M. 1 hit.
    PF08715. Viral_protease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101816. SSF101816. 1 hit.
    SSF144246. SSF144246. 1 hit.
    SSF50494. SSF50494. 1 hit.
    PROSITEi PS51442. M_PRO. 1 hit.
    PS51154. MACRO. 1 hit.
    PS51124. PEPTIDASE_C16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiR1A_BCRP3
    AccessioniPrimary (citable) accession number: P0C6T7
    Secondary accession number(s): Q3I5J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Bat coronavirus rp3 is highly similar to SARS-CoV (SARS-like).

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3