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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.1 Publication

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1533For PL-PRO activityPROSITE-ProRule annotation1
Active sitei1694For PL-PRO activityPROSITE-ProRule annotation1
Active sitei3144For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3248For 3CL-PRO activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1610 – 1647C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4168 – 4184By similarityAdd BLAST17
Zinc fingeri4211 – 4224By similarityAdd BLAST14

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiBat coronavirus HKU9 (BtCoV) (BtCoV/HKU9)
Taxonomic identifieri694006 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRousettus leschenaultii (Leschenault's rousette) [TaxID: 9408]
Proteomesi
  • UP000006576 Componenti: Genome

Subcellular locationi

Non-structural protein 7 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 8 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 9 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 10 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2040 – 2060HelicalSequence analysisAdd BLAST21
Transmembranei2081 – 2101HelicalSequence analysisAdd BLAST21
Transmembranei2162 – 2182HelicalSequence analysisAdd BLAST21
Transmembranei2183 – 2203HelicalSequence analysisAdd BLAST21
Transmembranei2218 – 2238HelicalSequence analysisAdd BLAST21
Transmembranei2621 – 2641HelicalSequence analysisAdd BLAST21
Transmembranei2719 – 2739HelicalSequence analysisAdd BLAST21
Transmembranei2865 – 2885HelicalSequence analysisAdd BLAST21
Transmembranei2887 – 2907HelicalSequence analysisAdd BLAST21
Transmembranei2916 – 2936HelicalSequence analysisAdd BLAST21
Transmembranei2946 – 2966HelicalSequence analysisAdd BLAST21
Transmembranei2970 – 2990HelicalSequence analysisAdd BLAST21
Transmembranei3423 – 3443HelicalSequence analysisAdd BLAST21
Transmembranei3449 – 3469HelicalSequence analysisAdd BLAST21
Transmembranei3474 – 3494HelicalSequence analysisAdd BLAST21
Transmembranei3517 – 3537HelicalSequence analysisAdd BLAST21
Transmembranei3569 – 3589HelicalSequence analysisAdd BLAST21
Transmembranei3592 – 3612HelicalSequence analysisAdd BLAST21
Transmembranei3620 – 3640HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003381101 – 4248Replicase polyprotein 1aAdd BLAST4248
ChainiPRO_00003381111 – 175Non-structural protein 1Sequence analysisAdd BLAST175
ChainiPRO_0000338112176 – 772Non-structural protein 2Sequence analysisAdd BLAST597
ChainiPRO_0000338113773 – 2609Non-structural protein 3Sequence analysisAdd BLAST1837
ChainiPRO_00003381142610 – 3103Non-structural protein 4Sequence analysisAdd BLAST494
ChainiPRO_00003381153104 – 34093C-like proteinaseSequence analysisAdd BLAST306
ChainiPRO_00003381163410 – 3699Non-structural protein 6Sequence analysisAdd BLAST290
ChainiPRO_00003381173700 – 3782Non-structural protein 7Sequence analysisAdd BLAST83
ChainiPRO_00003381183783 – 3982Non-structural protein 8Sequence analysisAdd BLAST200
ChainiPRO_00003381193983 – 4094Non-structural protein 9Sequence analysisAdd BLAST112
ChainiPRO_00003381214094 – 4248Non-structural protein 11Sequence analysisAdd BLAST155
ChainiPRO_00003381204095 – 4233Non-structural protein 10Sequence analysisAdd BLAST139

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei175 – 176CleavageSequence analysis2
Sitei772 – 773Cleavage; by PL-PROSequence analysis2
Sitei2609 – 2610Cleavage; by PL-PROSequence analysis2
Sitei3103 – 3104Cleavage; by 3CL-PROSequence analysis2
Sitei3409 – 3410Cleavage; by 3CL-PROSequence analysis2
Sitei3699 – 3700Cleavage; by 3CL-PROSequence analysis2
Sitei3782 – 3783Cleavage; by 3CL-PROSequence analysis2
Sitei3982 – 3983Cleavage; by 3CL-PROSequence analysis2
Sitei4094 – 4095Cleavage; by 3CL-PROSequence analysis2
Sitei4233 – 4234Cleavage; by 3CL-PROSequence analysis2

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6T6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini930 – 1097MacroPROSITE-ProRule annotationAdd BLAST168
Domaini1492 – 1757Peptidase C16PROSITE-ProRule annotationAdd BLAST266
Domaini3104 – 3409Peptidase C30PROSITE-ProRule annotationAdd BLAST306

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2015 – 2238HD1By similarityAdd BLAST224
Regioni2621 – 2990HD2By similarityAdd BLAST370
Regioni3423 – 3640HD3By similarityAdd BLAST218

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1610 – 1647C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4168 – 4184By similarityAdd BLAST17
Zinc fingeri4211 – 4224By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR032505. Corona_NSP4_C.
IPR002589. Macro_dom.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Replicase polyprotein 1a (identifier: P0C6T6-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGVPDPPKL KSMVVTTLKW CDPFANPNVT GWDIPIEEAL EYAKQQLRTP
60 70 80 90 100
EPQLVFVPYY LSHAPGISGD RVVITDSIWY ATNFGWQPIR ELAMDKDGVR
110 120 130 140 150
YGRGGTHGVL LPMQDPSFIM GDIDIQIRKY GIGANSPPDV LPLWDGFSDP
160 170 180 190 200
GPDVGPYLDF PDNCCPTKPK AKRGGDVYLS DQYGFDNNGI LVEPVMKLLG
210 220 230 240 250
VIKSDFTLEQ LLAALGKYRT EDGYDLPDGY VKVAIKVGRK AVPVLKQSIF
260 270 280 290 300
TVVGVTEQLV PGYYYPFSTS SVVEHTKPTR GGPVGKTVEA VMLSLYGTNN
310 320 330 340 350
YNPATPVARL KCSYCDYYGW TPLKDIGTVN CLCGAEFQLT SSCVDAESAG
360 370 380 390 400
VIKPGCVMLL DKSPGMRLIP GNRTYVSFGG AIWSPIGKVN GVTVWVPRAY
410 420 430 440 450
SIVAGEHSGA VGSGDTVAIN KELVEYLIEG IRVDADTLDN PTCATFIANL
460 470 480 490 500
DCDTKAPVVH TVESLQGLCL ANKIMLGDKP LPTDEFHPFI VGLAYHVQRA
510 520 530 540 550
CWYGALASRT FEAFRDFVRT EEERFAQFFG KVCAPINGCV YLAYTTGRVT
560 570 580 590 600
LFSAYQVLNT AIAKSKDAFG GVAAIVVDML KPILEWVLKK MSIAKGAWLP
610 620 630 640 650
YAEGLLALFK AQFTVVKGKF QFLRASLNSK CHSLCDLLTT IMSKLLTSVK
660 670 680 690 700
WAGCKVDALY TGTYYYFSRK GVLTEVQLCA KRLGLLLTPK QQKMEVEVLD
710 720 730 740 750
GDFDAPVTLT DLELEECTGV LEEVFGASDV KLVKGTLVSL ASKLFVRTED
760 770 780 790 800
GFLYRYVKSG GVLGKAFRLR GGGVSKVTFG DEEVHTIPNT VTVNFSYDVC
810 820 830 840 850
EGLDAILDKV MAPFQVEEGT KLEDLACVVQ KAVYERLSDL FSDCPAELRP
860 870 880 890 900
INLEDFLTSE CFVYSKDYEK ILMPEMYFSL EDAVPVDDEM VDDIEDTVEQ
910 920 930 940 950
ASDSDDQWLG DEGAEDCDNT IQDVDVATSM TTPCGYTKIA EHVYIKCADI
960 970 980 990 1000
VQEARNYSYA VLVNAANVNL HHGGGVAGAL NRATNNAMQK ESSEYIKANG
1010 1020 1030 1040 1050
SLQPGGHVLL SSHGLASHGI LHVVGPDKRL GQDLALLDAV YAAYTGFDSV
1060 1070 1080 1090 1100
LTPLVSAGIF GFTVEESLCS LVKNVACTTY VVVYDRQLYE RALATSFDVP
1110 1120 1130 1140 1150
GPQSSVQHVP AIDWAEAVEV QESIVDQVET PSLGAVDTVD SNADSGLNET
1160 1170 1180 1190 1200
ARSPENVVGS VPDDVVADVE SCVRDLVRQV VKKVKRDKRP PPIVPQQTVE
1210 1220 1230 1240 1250
QQPQEISSPG DCNTVLVDVV SMSFSAMVNF GKEKGLLIPV VIDYPAFLKV
1260 1270 1280 1290 1300
LKRFSPKEGL FSSNGYEFYG YSRDKPLHEV SKDLNSLGRP LIMIPFGFIV
1310 1320 1330 1340 1350
NGQTLAVSAV SMRGLTVPHT VVVPSESSVP LYRAYFNGVF SGDTTAVQDF
1360 1370 1380 1390 1400
VVDILLNGAR DWDVLQTTCT VDRKVYKTIC KRGNTYLCFD DTNLYAITGD
1410 1420 1430 1440 1450
VVLKFATVSK ARAYLETKLC APEPLIKVLT TVDGINYSTV LVSTAQSYRA
1460 1470 1480 1490 1500
QIGTVFCDGH DWSNKNPMPT DEGTHLYKQD NFSSAEVTAI REYYGVDDSN
1510 1520 1530 1540 1550
IIARAMSIRK TVQTWPYTVV DGRVLLAQRD SNCYLNVAIS LLQDIDVSFS
1560 1570 1580 1590 1600
TPWVCRAYDA LKGGNPLPMA EVLIALGKAT PGVSDDAHMV LSAVLNHGTV
1610 1620 1630 1640 1650
TARRVMQTVC EHCGVSQMVF TGTDACTFYG SVVLDDLYAP VSVVCQCGRP
1660 1670 1680 1690 1700
AIRYVSEQKS PWLLMSCTPT QVPLDTSGIW KTAIVFRGPV TAGHYMYAVN
1710 1720 1730 1740 1750
GTLISVYDAN TRRRTSDLKL PATDILYGPT SFTSDSKVET YYLDGVKRTT
1760 1770 1780 1790 1800
IDPDFSKYVK RGDYYFTTAP IEVVAAPKLV TSYDGFYLSS CQNPQLAESF
1810 1820 1830 1840 1850
NKAINATKTG PMKLLTMYPN VAGDVVAISD DNVVAHPYGS LHMGKPVLFV
1860 1870 1880 1890 1900
TRPNTWKKLV PLLSTVVVNT PNTYDVLAVD PLPVNNETSE EPISVKAPIP
1910 1920 1930 1940 1950
LYGLKATMVL NGTTYVPGNK GHLLCLKEFT LTDLQTFYVE GVQPFVLLKA
1960 1970 1980 1990 2000
SHLSKVLGLR VSDSSLHVNH LSKGVVYAYA ATRLTTRVTT SLLGGLVTRS
2010 2020 2030 2040 2050
VRKTADFVRS TNPGSKCVGL LCLFYQLFMR FWLLVKKPPI VKVSGIIAYN
2060 2070 2080 2090 2100
TGCGVTTCVL NYLRSRCGNI SWSRLLKLLR YMLYIWFVWT CLTICGVWLS
2110 2120 2130 2140 2150
EPYAPSLVTR FKYFLGIVMP CDYVLVNETG TGWLHHLCMA GMDSLDYPAL
2160 2170 2180 2190 2200
RMQQHRYGSP YNYTYILMLL EAFFAYLLYT PALPIVGILA VLHLIVLYLP
2210 2220 2230 2240 2250
IPLGNSWLVV FLYYIIRLVP FTSMLRMYIV IAFLWLCYKG FLHVRYGCNN
2260 2270 2280 2290 2300
VACLMCYKKN VAKRIECSTV VNGVKRMFYV NANGGTHFCT KHNWNCVSCD
2310 2320 2330 2340 2350
TYTVDSTFIC RQVALDLSAQ FKRPIIHTDE AYYEVTSVEV RNGYVYCYFE
2360 2370 2380 2390 2400
SDGQRSYERF PMDAFTNVSK LHYSELKGAA PAFNVLVFDA TNRIEENAVK
2410 2420 2430 2440 2450
TAAIYYAQLA CKPILLVDKR MVGVVGDDAT IARAMFEAYA QNYLLKYSIA
2460 2470 2480 2490 2500
MDKVKHLYST ALQQISSGMT VESVLKVFVG STRAEAKDLE SDVDTNDLVS
2510 2520 2530 2540 2550
CIRLCHQEGW EWTTDSWNNL VPTYIKQDTL STLEVGQFMT ANAKYVNANI
2560 2570 2580 2590 2600
AKGAAVNLIW RYADFIKLSE SMRRQLKVAA RKTGLNLLVT TSSLKADVPC
2610 2620 2630 2640 2650
MVTPFKIIGG HRRIVSWRRV LIHVFMLLVV LNPQWFTPWY IMRPIEYNVV
2660 2670 2680 2690 2700
DFKVIDNAVI RDITSADQCF ANKFSAFENW YSNRYGSYVN SRGCPMVVGV
2710 2720 2730 2740 2750
VSDIVGSLVP GLPARFLRVG TTLLPLVNYG LGAVGSVCYT PHYAINYDVF
2760 2770 2780 2790 2800
DTSACVLAAT CTLFSSASGE RMPYCADAAL IQNASRYDML KPHVMYPFYE
2810 2820 2830 2840 2850
HSGYIRFPEV ISAGVHIVRT MAMEYCKVGR CDVSEAGLCM SLQPRWVVNN
2860 2870 2880 2890 2900
AYFRQQSGVY CGTSAFDLFM NMLLPIFTPV GAVDITTSIL MGALLAVVVS
2910 2920 2930 2940 2950
MSLYYLLRFR RAFGDYSGVI FTNILAFVLN VIVLCLEGPY PMLPSIYAMV
2960 2970 2980 2990 3000
FLYATCYFGS DIACMMHVSF LIMFAGVVPL WVTVLYIVVV LSRHILWFAS
3010 3020 3030 3040 3050
LCTKRTVQVG DLAFHSFQDA ALQTFMLDKE VFLRLKREIS SDAYFKYLAM
3060 3070 3080 3090 3100
YNKYKYYSGP MDTAAYREAA CSHLVMALEK YSNGGGDTIY QPPRCSVASA
3110 3120 3130 3140 3150
ALQAGLTRMA HPSGLVEPCL VKVNYGSMTL NGIWLDNFVI CPRHVMCSRD
3160 3170 3180 3190 3200
ELANPDYPRL SMRAANYDFH VSQNGHNIRV IGHTMEGSLL KLTVDVNNPK
3210 3220 3230 3240 3250
TPAYSFIRVS TGQAMSLLAC YDGLPTGVYT CTLRSNGTMR ASFLCGSCGS
3260 3270 3280 3290 3300
PGFVMNGKEV QFCYLHQLEL PNGTHTGTDF SGVFYGPFED KQVPQLAAPD
3310 3320 3330 3340 3350
CTITVNVLAW LYAAVLSGEN WFLTKSSISP AEFNNCAVKY MCQSVTSESL
3360 3370 3380 3390 3400
QVLQPLAAKT GISVERMLSA LKVLLSAGFC GRTIMGSCSL EDEHTPYDIG
3410 3420 3430 3440 3450
RQMLGVKLQG KFQSMFRWTL QWFAIIFVLT ILILLQLAQW TFVGALPFTL
3460 3470 3480 3490 3500
LLPLIGFVAV CVGFVSLLIK HKHTYLTVYL LPVAMVTAYY NFQYTPEGVQ
3510 3520 3530 3540 3550
GYLLSLYNYV NPGRIDVIGT DLLTMLIISV ACTLLSVRMV RTDAYSRIWY
3560 3570 3580 3590 3600
VCTAVGWLYN CWTGSADTVA ISYLTFMVSV FTNYTGVACA SLYAAQFMVW
3610 3620 3630 3640 3650
VLKFLDPTIL LLYGRFRCVL VCYLLVGYLC TCYFGVFNLI NRLFRCTLGN
3660 3670 3680 3690 3700
YEYVVSSQEL RYMNSHGLLP PTNSWQALML NIKLAGIGGI PIYRVSTIQS
3710 3720 3730 3740 3750
NMTDLKCTSV VLLSVLQQLR VESSSKLWAL CVKLHNEILA SNSPTEAFEA
3760 3770 3780 3790 3800
FVSLLSVLLS LPGAINLDEL CSSILENNSV LQAVASEFSN LSSYVDYENA
3810 3820 3830 3840 3850
QKAYDTAVAT GAPASTVNAL KKAMNVAKSV LDKDVATTRK LERMSELAMT
3860 3870 3880 3890 3900
AMYKQARAED RRSKVTAAMQ TMLFNMIRRL DSDALSNILN NARNGVVPLG
3910 3920 3930 3940 3950
VIPRTAANKL LLVVPDFSVY TATITMPTLT YAGSAWDVMQ VADADGKTVN
3960 3970 3980 3990 4000
ATDITRENSV NLAWPLVVTA QRQQATSPVK LQNNELMPQT VKRMNVVAGV
4010 4020 4030 4040 4050
SQTACVTDAV AYYNATKEGR HVMAILADTD GLAFAKVEKS TGDGFVILEL
4060 4070 4080 4090 4100
EPPCKFMVDT PKGPALKYLY FTKGLKNLCR GTVLGTLACT VRLHAGSATE
4110 4120 4130 4140 4150
VASNSSILSL CSFSVDPEAT YKDYLDNGGS PIGNCVKMLT PHTGTGLAIT
4160 4170 4180 4190 4200
AKPDANIDQE SFGGASCCLY CRCHIEHPGA SGVCKYKGKF VQIPLVGVND
4210 4220 4230 4240
PIGFCIRNVV CAVCNMWQGY GCPCSSLREI NLQARDECFL NESGVLVE
Note: Produced by conventional translation.
Length:4,248
Mass (Da):468,590
Last modified:June 10, 2008 - v1
Checksum:iC0D0584A862070A7
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6W5-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6W5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Length:6,930
Mass (Da):769,729
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065513 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065513 Genomic RNA. No translation available.

3D structure databases

ProteinModelPortaliP0C6T6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR032505. Corona_NSP4_C.
IPR002589. Macro_dom.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
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Entry informationi

Entry nameiR1A_BCHK9
AccessioniPrimary (citable) accession number: P0C6T6
Secondary accession number(s): A3EXG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.