P0C6T5 (R1A_BCHK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replicase polyprotein 1a
Short name=pp1a

Alternative name(s):
ORF1a polyprotein

Cleaved into the following 11 chains:

1. Non-structural protein 1
   Short name=nsp1
   Alternative name(s):
   Leader protein
2. Non-structural protein 2
   Short name=nsp2
   Alternative name(s):
   p65 homolog
3. Non-structural protein 3
   Short name=nsp3
   EC=3.4.19.12
   EC=3.4.22.69
   Alternative name(s):
   PL2-PRO
   Papain-like proteinase
   Short name=PL-PRO
4. Non-structural protein 4
   Short name=nsp4
5. 3C-like proteinase
   Short name=3CL-PRO
   Short name=3CLp
   EC=3.4.22.-
   Alternative name(s):
   nsp5
6. Non-structural protein 6
   Short name=nsp6
7. Non-structural protein 7
   Short name=nsp7
8. Non-structural protein 8
   Short name=nsp8
9. Non-structural protein 9
   Short name=nsp9
10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
11. Non-structural protein 11
    Short name=nsp11

Gene names

ORF Names:

1a

Organism

Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004) [Complete proteome]

Taxonomic identifier

694008 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirinae › Betacoronavirus

Virus host

Pipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus) [TaxID: 105295]

Protein attributes

Sequence length	4481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity. The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity. Nsp9 is a ssRNA-binding protein By similarity.
Catalytic activity	TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.
Subcellular location	Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential. Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential. Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential. Non-structural protein 7: Host cytoplasm › host perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Non-structural protein 8: Host cytoplasm › host perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Non-structural protein 9: Host cytoplasm › host perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity. Non-structural protein 10: Host cytoplasm › host perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Domain	The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.
Post-translational modification	Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.
Sequence similarities	Belongs to the coronaviruses polyprotein 1ab family. Contains 1 Macro domain. Contains 1 peptidase C16 domain. Contains 1 peptidase C30 domain.

Ontologies

Keywords
Biological process	Activation of host autophagy by virus Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host IRF3 by virus Inhibition of host ISG15 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Modulation of host ubiquitin pathway by viral deubiquitinase Modulation of host ubiquitin pathway by virus Ubl conjugation pathway Viral immunoevasion
Cellular component	Host cytoplasm Host membrane Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Repeat Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	induction by virus of host autophagy Inferred from electronic annotation. Source: UniProtKB-KW modulation by virus of host protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host IRF3 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host ISG15 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro viral protein processing Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral replication complex Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro omega peptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform Replicase polyprotein 1a (identifier: P0C6T5-1)

Also known as: pp1a; ORF1a polyprotein;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by conventional translation.

Isoform Replicase polyprotein 1ab (identifier: P0C6W4-1)

Also known as: pp1ab;

The sequence of this isoform can be found in the external entry P0C6W4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 4481	4481	Replicase polyprotein 1a		PRO_0000338098
	Chain	1 – 195	195	Non-structural protein 1 Potential		PRO_0000338099
	Chain	196 – 851	656	Non-structural protein 2 Potential		PRO_0000338100
	Chain	852 – 2830	1979	Non-structural protein 3 Potential		PRO_0000338101
	Chain	2831 – 3338	508	Non-structural protein 4 Potential		PRO_0000338102
	Chain	3339 – 3644	306	3C-like proteinase Potential		PRO_0000338103
	Chain	3645 – 3936	292	Non-structural protein 6 Potential		PRO_0000338104
	Chain	3937 – 4019	83	Non-structural protein 7 Potential		PRO_0000338105
	Chain	4020 – 4218	199	Non-structural protein 8 Potential		PRO_0000338106
	Chain	4219 – 4328	110	Non-structural protein 9 Potential		PRO_0000338107
	Chain	4328 – 4481	154	Non-structural protein 11 Potential		PRO_0000338109
	Chain	4329 – 4467	139	Non-structural protein 10 Potential		PRO_0000338108
Regions
	Transmembrane	2196 – 2216	21	Helical; Potential
	Transmembrane	2268 – 2288	21	Helical; Potential
	Transmembrane	2372 – 2392	21	Helical; Potential
	Transmembrane	2396 – 2416	21	Helical; Potential
	Transmembrane	2421 – 2441	21	Helical; Potential
	Transmembrane	2848 – 2868	21	Helical; Potential
	Transmembrane	3119 – 3139	21	Helical; Potential
	Transmembrane	3152 – 3172	21	Helical; Potential
	Transmembrane	3203 – 3223	21	Helical; Potential
	Transmembrane	3650 – 3670	21	Helical; Potential
	Transmembrane	3684 – 3704	21	Helical; Potential
	Transmembrane	3709 – 3729	21	Helical; Potential
	Transmembrane	3760 – 3777	18	Helical; Potential
	Transmembrane	3782 – 3802	21	Helical; Potential
	Transmembrane	3823 – 3843	21	Helical; Potential
	Transmembrane	3855 – 3875	21	Helical; Potential
	Domain	1186 – 1345	160	Macro
	Domain	1628 – 1902	275	Peptidase C16
	Domain	3339 – 3644	306	Peptidase C30
	Zinc finger	1748 – 1785	38	C4-type By similarity
	Zinc finger	4402 – 4418	17	By similarity
	Zinc finger	4444 – 4457	14	By similarity
	Region	2158 – 2441	284	HD1 By similarity
	Region	2848 – 3223	376	HD2 By similarity
	Region	3650 – 3875	226	HD3 By similarity
	Compositional bias	964 – 1066	103	Glu-rich
Sites
	Active site	1668	1	For PL-PRO activity By similarity
	Active site	1838	1	For PL-PRO activity By similarity
	Active site	3379	1	For 3CL-PRO activity By similarity
	Active site	3486	1	For 3CL-PRO activity By similarity
	Site	195 – 196	2	Cleavage Potential
	Site	851 – 852	2	Cleavage; by PL-PRO Potential
	Site	2830 – 2831	2	Cleavage; by PL-PRO Potential
	Site	3338 – 3339	2	Cleavage; by 3CL-PRO Potential
	Site	3644 – 3645	2	Cleavage; by 3CL-PRO Potential
	Site	3936 – 3937	2	Cleavage; by 3CL-PRO Potential
	Site	4019 – 4020	2	Cleavage; by 3CL-PRO Potential
	Site	4218 – 4219	2	Cleavage; by 3CL-PRO Potential
	Site	4328 – 4329	2	Cleavage; by 3CL-PRO Potential
	Site	4467 – 4468	2	Cleavage; by 3CL-PRO Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 6EA797D2808AF75F	FASTA	4,481	494,801
	10 20 30 40 50 60 MSFVAGVAPQ GARGKYRAEL NTEKRTDHVS LKASLCDAGD LVLKISPWFM DGESAYKHVS 70 80 90 100 110 120 EQLSKGSKLL FVPQTLKGFI RHLPGPRVYL VERLTGGTYS DPFMVNQLAY QNAAGEGVIG 130 140 150 160 170 180 TTLQGKRVGM FFPFDADLVT GEFQFLLRKK GFGGNRFRDA PWDYNWTPYS DLMDALEADP 190 200 210 220 230 240 CGKYSQSLLK KLVGGDFTPI DQYMCGKNGK PIAEFAALMA SEGITKLADV EAEVKSRTDS 250 260 270 280 290 300 DRYIVFKNKL YRIVWNVQRK DVAYSKQSAF TMNSIVQLDT MEDVPRHSFT IGSEIQVIAP 310 320 330 340 350 360 STAVQANGHL NLKQRLLYAF YGKQAVSEPN YIYHSAYVDC TSCGKGSWLT GNAVQGFACD 370 380 390 400 410 420 CGAHYCANDV DLQSSGLVRK NAVLLTTCPC NKDGECKHTL PQLVSMMTDK CDVEVVGKTF 430 440 450 460 470 480 ILTYGGVIYA YMGCSGGTMH FIPRAKSCVS KIGDAIFTGC TGTWSKVCET ANLFLERAQH 490 500 510 520 530 540 AINFVNEFVL TETVVALLSG TTSSIEELRD LCRNATFEKV RDYLTPRGWI VTMGSYIEGV 550 560 570 580 590 600 INVGAAGVCN AALNAPFIVL SGLGESFKKV AATPWKLCSS LRETLDHYAD SITYRVFPYD 610 620 630 640 650 660 IPCDVTDYTA LLLDCAVLTG ASAYFVARYV DEKVEQLTNL VFSSCQSAVA AFVQACMSTY 670 680 690 700 710 720 KATAKFISDM FTLIKVVSER LYVYTSVGFV VVGDYSSQLL KQFMHILSKA MQLLHTTVSW 730 740 750 760 770 780 AGSKLPSVVY NGRDSLVFPS GTYYCVSTQG RSLQDQFDLV IPGDLSKKQI GILEPTPNST 790 800 810 820 830 840 TVDKKINTNV VEVVVGQLEP TKEHSPELVV GDYVIISNKI FVRSVEDSET VFYPLCTDGK 850 860 870 880 890 900 IVPTLFRLKG GAPPKGVKFG GEQTKEITAV RSVSVDYDVH PVLDALLAGS ELATFTVEKD 910 920 930 940 950 960 LPVKDFVDVV KDEVIELLSK LLRGYNVDGF DLEDFADTPC YVYNAEGDLA WSSTMTFSVN 970 980 990 1000 1010 1020 PVEEVEEECD DDYVEDEYLS EEMLVEEDEN SWAAAVEAVI PMEDVQLDTL VAEIDVSEPA 1030 1040 1050 1060 1070 1080 DDVAEQASTE EVEVPSACVL EASQVANAAE VESCEAEVSS SIPLHEDANA AKANDCAEGM 1090 1100 1110 1120 1130 1140 PALDSTETVS KLSVDTPVGD VTQDDATSSN ATVISEDVHT ATHSKGLVAV PEVVPEKALG 1150 1160 1170 1180 1190 1200 TSVERMRSTS EWTVVETSLK QETAVIVKND SSAKPQRVKK PKAENPLKNF KHIVLNNDVT 1210 1220 1230 1240 1250 1260 LVFGDAIAVA RATEDCILVN AANTHLKHGG GIAAAIDRAS GGLVQAESDD YVNFYGPLNV 1270 1280 1290 1300 1310 1320 GDSTLLKGHG LATGILHVVG PDARANQDIQ LLKRCYKAFN KYPLVVSPLI SAGIFCVEPR 1330 1340 1350 1360 1370 1380 VSLEYLLSVV HTKTYVVVNS EKVYNDLAAP KPPTGLTYSH EGWRGIIRNA KSFGFTCFIC 1390 1400 1410 1420 1430 1440 TDQSANAKLL KGRGVDLTKK TQTVDGVKYY LYSSKDPLTD IITAANACKG ICAMPIGYVT 1450 1460 1470 1480 1490 1500 HGLDLAQAGQ QVKKITVPYV CLLASKDQVP ILNSDVAVQT PEQSFINTVI ANGGYHCWHL 1510 1520 1530 1540 1550 1560 VTGELIVKGV SYRKLLNWSD QTICYADNKF YVVKGQIALP FDSLEKCRTY LTSRAAQQKN 1570 1580 1590 1600 1610 1620 VDVLVTIDGV NFRTVVLNNT TTYRVQLGSV FYKGSDISDT IPTEKMSGEA VYLADNLSEA 1630 1640 1650 1660 1670 1680 EKAVLSEVYG TADTAFLHRY YSLLALVKKW KYTVHDGVKS LKLNSNNCYV NVTMLMLDML 1690 1700 1710 1720 1730 1740 KEIKFIVPAL QAAYLKHKGG DSTEFIALIM AYGDCTYGEP DDASRLLHTI LSKAELTTQA 1750 1760 1770 1780 1790 1800 KMVWRQWCNV CGVQDTTTTG LKACIYVGMN SLDELHATHE ECCQCGDVRK RQLVEHNAPW 1810 1820 1830 1840 1850 1860 LLLSGLNEAK VMTPTSQSAG PDYTAFNVFQ GVETSVGHYL HVRVKDNLLY KYDSGSLSKT 1870 1880 1890 1900 1910 1920 SDMKCKMTDV YYPKQRYSAD CNVVVYSLDG NTWADVDPDL SAFYMKDGKY FTKKPVIEYS 1930 1940 1950 1960 1970 1980 PATILSGSVY TNSCLVGHDG TIGSDAISSS FNNLLGFDNS KPVSKKLTYS FFPDFEGDVI 1990 2000 2010 2020 2030 2040 LTEYSTYDPI YKNGAMLHGK PILWVNNSKF DSALNKFNRA TLRQVYDIAP VTLENKYTVL 2050 2060 2070 2080 2090 2100 QDNQIQQVEV EAPKEDAKPQ SPVQVAEDID NKLPIIKCKG LKKPFVKDGY SFVNDPQGVN 2110 2120 2130 2140 2150 2160 VIDTLGIDDL RALYVDRNLR LIVLKENNWS ALFNIHTVEK GDLSVIAASG SITRRVKILL 2170 2180 2190 2200 2210 2220 GASSLFAQFA SVTVNVTTAM GKALGRMTRN VITNTGIIGQ GFALLKMLLI LPFTFWKSKN 2230 2240 2250 2260 2270 2280 QSTVKVEVGA LRTAGIVTTN VVKQCASAAY DVLVVKFKRI DWKSTLRLLF LICTTGLLLS 2290 2300 2310 2320 2330 2340 SLYYLFLFHQ VLTSDVMLDG AEGMLATYRE LRSYLGIHSL CDGMVEAYRN VSYDVNDFCS 2350 2360 2370 2380 2390 2400 NRSALCNWCL IGQDSLTRYS AFQMIQTHVT SYVINIDWVW FVMEFALAYV LYTSTFNVLL 2410 2420 2430 2440 2450 2460 LVVSSQYFFS YTGAFVNWRS YNYLVSGYFF CVTHIPLLGL VRIYNFLACL WFLRRFYNHV 2470 2480 2490 2500 2510 2520 INGCKDTACL LCYKRNRLTR VEASTVVCGS KRTFYIVANG GTSFCCRHNW NCVDCDTAGI 2530 2540 2550 2560 2570 2580 GNTFICEEVA NDLTTSLRRL VKPTDKSHYY VESVTVKDSV VQLHYSREGA SCYERYPLCY 2590 2600 2610 2620 2630 2640 FTNLDKLKFK EVCKTPTGIP EHNFLIYDSS DRGQENLARS ACVYYSQVLS KPMLLVDSNM 2650 2660 2670 2680 2690 2700 VTTVGDSREI ASKMLDSYVN SFISLFGVNR DKLDKLVATA RDCVKRGDDF QTVIKTFTDA 2710 2720 2730 2740 2750 2760 ARGPAGVESD VETSSIVDAL QYAYKHDLQL TTEGFNNYVP SYIKPDSVAT ADLGCLIDLN 2770 2780 2790 2800 2810 2820 AASVNQTSIR NANGACIWNS SDYMKLSDSL KRQIRIACRK CNIPFRLTTS RLRSADNILS 2830 2840 2850 2860 2870 2880 VKFSATKLSG GAPKWLLKLR DFTWKSYCVV TLVVFAMAVL SYLCLPAFNM SQVSFHEDRI 2890 2900 2910 2920 2930 2940 LTYKVVENGI IRDITPSDTC FANKYQSFSK WFNEHYGGLF NNDISCPVTV AVIAGVAGAR 2950 2960 2970 2980 2990 3000 VPNLPANVAW VGRQIVLFVS RVFASSNNVC YTPTAEIPYE RFSDSGCVLA SECTLFRDAE 3010 3020 3030 3040 3050 3060 GKINPYCYDP TVLPGASAYD QMKPHVRYDM YDSDMYIKFP EVVFESTLRI TKTLATRYCR 3070 3080 3090 3100 3110 3120 FGSCEDANEG VCITTNGSWA IYNDHYANKP GVYCGDNYFD IVRRLGLSLF QPVTYFQLST 3130 3140 3150 3160 3170 3180 SLALGVMLCI FLTIAFYYVN KVKRALADYT QCAVVAVAAA LLNSLCLCFV VSNPLLVLPY 3190 3200 3210 3220 3230 3240 TALYYYATFY LTGEPAFVMH VSWFVMFGTV VPIWMVFAYI VGVCLRHLLW VMAYFSKKHV 3250 3260 3270 3280 3290 3300 EVFTDGKLNC SFQDAAANIF VINKDTYVAL RNSITQDSYN RYLSMFNKYK YYSGAMDTAS 3310 3320 3330 3340 3350 3360 YREASAAHLC KALQVYSETG SDVLFQPPNC SVTSSVLQSG LVKMAAPSGV VENCMVQVTC 3370 3380 3390 3400 3410 3420 GSMTLNGLWL DNYVWCPRHV MCPADQLSDP NYDALLVSKT NLSFIVQKNV GAPANLRVVG 3430 3440 3450 3460 3470 3480 HTMVGTLLKL TVESANPQTP AYTFTTVKPG ASFSVLACYN GRPTGVFMVN MRQNSTIKGS 3490 3500 3510 3520 3530 3540 FLCGSCGSVG YTQEGNVINF CYMHQMELSN GTHTGCAFDG VMYGAFEDRQ VHQVQLSDKY 3550 3560 3570 3580 3590 3600 CTINIVAWLY AAILNGCNWF VKPNKTGIAT FNEWAMSNQF TEFIGTQSVD MLAHKTGVSV 3610 3620 3630 3640 3650 3660 EQLLYAIQTL HKGFQGKTIL GNSMLEDEFT PDDVNMQVMG VVMQSGVKRI SYGLVHWLFT 3670 3680 3690 3700 3710 3720 TLLLAYVATL QLTKFTIWNY LFEVIPLQLT PLVLCVMACV MLTVKHKHTF LTLFLLPTAI 3730 3740 3750 3760 3770 3780 CLTYANIVYE PQTPVSSALI AVANWLNPAS VYMRTTHTDL GVYLSLCFAL AVVVRRLYRP 3790 3800 3810 3820 3830 3840 NASNLALALG SAMVWFYTYT TGDCSSPLTY LMFLTTLTSD YTVTVFLAVN VAKFFARVVF 3850 3860 3870 3880 3890 3900 LYAPHAGFIF PEVKLVLLMY LAVGYFCTVY FGVFSLLNLK LRVPLGVYDY TVSTQEFRYL 3910 3920 3930 3940 3950 3960 TGNGLHAPRN SWEALRLNMK LIGIGGTPCI KIASVQSKLT DLKCTSVVLL SVLQQLHLEA 3970 3980 3990 4000 4010 4020 NSKAWAHCVK LHNDILAATD PTEAFDNFVC LFATLMSFSA NVDLEALASD LLDHPSVLQA 4030 4040 4050 4060 4070 4080 TLSEFSHLAS YAELEAAQSS YQKALNSGDA SPQVLKALQK AVNIAKNAYE KDKAVARKLE 4090 4100 4110 4120 4130 4140 RMAEQAMTSM YKQARAEDKK AKIVSAMQTM LFGMIKKLDN DVLNGVISNA RNGCVPLSVV 4150 4160 4170 4180 4190 4200 PLCASNKLRV VIPDITIWNK VVTWPSLSYA GALWDISLIN NVDGEVVKSS DVTETNESLT 4210 4220 4230 4240 4250 4260 WPLVLECTRA ASSAVTLQNN EIRPSGLKTM VVSAGIDHAN CNTSSLAYYE PVEGRKMLMG 4270 4280 4290 4300 4310 4320 ILSENAHLKW AKVEGRDGFV NIELQPPCKF LIAGPKGPEV RYLYFVKNLN NLHRGQLLGH 4330 4340 4350 4360 4370 4380 IAATVRLQAG SNTEFAINSS VLSAVTFSVD PGKAYLDFVN AGGAPLTNCV KMLTPKTGTG 4390 4400 4410 4420 4430 4440 IAVSVKPEAN ADQDTYGGAS VCLYCRAHIE HPDVTGVCKF KGKFVQVPLH IRDPVGFCLQ 4450 4460 4470 4480 NTPCNVCQFW IGHGCNCDAL RGTTIPQSKD SNFLNESGVL L
	Isoform Replicase polyprotein 1ab (pp1ab) [UniParc]. See P0C6W4.		–	–

References

[1]

"Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features." Woo P.C.Y., Wang M., Lau S.K.P., Xu H.F., Poon R.W.S., Guo R., Wong B.H.L., Gao K., Tsoi H.-W., Huang Y., Li K.S.M., Lam C.S.F., Chan K.-H., Zheng B.-J., Yuen K.-Y. J. Virol. 81:1574-1585(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate HKU5-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF065509 Genomic RNA. No translation available.
3D structure databases
ProteinModelPortal	P0C6T5.
SMR	P0C6T5. Positions 3937-4007, 4268-4328, 4335-4459.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002589. A1pp. IPR024375. Nsp3_coronavir. IPR014828. NSP7. IPR014829. NSP8. IPR014822. NSP9. IPR008740. Peptidase_C30. IPR013016. Peptidase_C30/C16. IPR018995. RNA_synth_NSP10_coronavirus. IPR009003. Trypsin-like_Pept_dom. IPR014827. Viral_protease. [Graphical view]
Pfam	PF01661. Macro. 1 hit. PF09401. NSP10. 1 hit. PF11633. Nsp3. 1 hit. PF08716. nsp7. 1 hit. PF08717. nsp8. 1 hit. PF08710. nsp9. 1 hit. PF05409. Peptidase_C30. 1 hit. PF08715. Viral_protease. 1 hit. [Graphical view]
SUPFAM	SSF101816. Nsp9. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit. SSF144246. SSF144246. 1 hit.
PROSITE	PS51442. M_PRO. 1 hit. PS51154. MACRO. 1 hit. PS51124. PEPTIDASE_C16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

R1A_BCHK5

Accession

Primary (citable) accession number: P0C6T5
Secondary accession number(s): A3EXC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	April 3, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program