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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1634For PL-PRO activityPROSITE-ProRule annotation1
Active sitei1800For PL-PRO activityPROSITE-ProRule annotation1
Active sitei3332For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3439For 3CL-PRO activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1714 – 1751C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4355 – 4371By similarityAdd BLAST17
Zinc fingeri4397 – 4410By similarityAdd BLAST14

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiBat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004)
Taxonomic identifieri694007 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiTylonycteris pachypus (Lesser bamboo bat) [TaxID: 258959]
Proteomesi
  • UP000006574 Componenti: Genome

Subcellular locationi

Non-structural protein 7 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 8 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 9 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Non-structural protein 10 :
  • Host cytoplasmhost perinuclear region By similarity

  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2145 – 2165HelicalSequence analysisAdd BLAST21
Transmembranei2222 – 2242HelicalSequence analysisAdd BLAST21
Transmembranei2326 – 2346HelicalSequence analysisAdd BLAST21
Transmembranei2350 – 2370HelicalSequence analysisAdd BLAST21
Transmembranei2375 – 2395HelicalSequence analysisAdd BLAST21
Transmembranei2800 – 2820HelicalSequence analysisAdd BLAST21
Transmembranei3072 – 3092HelicalSequence analysisAdd BLAST21
Transmembranei3105 – 3125HelicalSequence analysisAdd BLAST21
Transmembranei3149 – 3169HelicalSequence analysisAdd BLAST21
Transmembranei3603 – 3623HelicalSequence analysisAdd BLAST21
Transmembranei3637 – 3657HelicalSequence analysisAdd BLAST21
Transmembranei3662 – 3682HelicalSequence analysisAdd BLAST21
Transmembranei3707 – 3727HelicalSequence analysisAdd BLAST21
Transmembranei3735 – 3755HelicalSequence analysisAdd BLAST21
Transmembranei3784 – 3804HelicalSequence analysisAdd BLAST21
Transmembranei3808 – 3828HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003380861 – 4434Replicase polyprotein 1aAdd BLAST4434
ChainiPRO_00003380871 – 195Non-structural protein 1Sequence analysisAdd BLAST195
ChainiPRO_0000338088196 – 847Non-structural protein 2Sequence analysisAdd BLAST652
ChainiPRO_0000338089848 – 2784Non-structural protein 3Sequence analysisAdd BLAST1937
ChainiPRO_00003380902785 – 3291Non-structural protein 4Sequence analysisAdd BLAST507
ChainiPRO_00003380913292 – 35973C-like proteinaseSequence analysisAdd BLAST306
ChainiPRO_00003380923598 – 3889Non-structural protein 6Sequence analysisAdd BLAST292
ChainiPRO_00003380933890 – 3972Non-structural protein 7Sequence analysisAdd BLAST83
ChainiPRO_00003380943973 – 4171Non-structural protein 8Sequence analysisAdd BLAST199
ChainiPRO_00003380954172 – 4281Non-structural protein 9Sequence analysisAdd BLAST110
ChainiPRO_00003380974281 – 4434Non-structural protein 11Sequence analysisAdd BLAST154
ChainiPRO_00003380964282 – 4420Non-structural protein 10Sequence analysisAdd BLAST139

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei195 – 196CleavageSequence analysis2
Sitei847 – 848Cleavage; by PL-PROSequence analysis2
Sitei2784 – 2785Cleavage; by PL-PROSequence analysis2
Sitei3291 – 3292Cleavage; by 3CL-PROSequence analysis2
Sitei3597 – 3598Cleavage; by 3CL-PROSequence analysis2
Sitei3889 – 3890Cleavage; by 3CL-PROSequence analysis2
Sitei3972 – 3973Cleavage; by 3CL-PROSequence analysis2
Sitei4171 – 4172Cleavage; by 3CL-PROSequence analysis2
Sitei4281 – 4282Cleavage; by 3CL-PROSequence analysis2
Sitei4420 – 4421Cleavage; by 3CL-PROSequence analysis2

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

Secondary structure

14434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3302 – 3305Combined sources4
Beta strandi3308 – 3313Combined sources6
Beta strandi3316 – 3323Combined sources8
Beta strandi3326 – 3330Combined sources5
Helixi3331 – 3334Combined sources4
Helixi3337 – 3339Combined sources3
Helixi3345 – 3350Combined sources6
Helixi3354 – 3356Combined sources3
Beta strandi3358 – 3360Combined sources3
Beta strandi3363 – 3365Combined sources3
Beta strandi3367 – 3369Combined sources3
Beta strandi3371 – 3377Combined sources7
Beta strandi3380 – 3387Combined sources8
Beta strandi3394 – 3397Combined sources4
Beta strandi3405 – 3412Combined sources8
Beta strandi3415 – 3423Combined sources9
Beta strandi3442 – 3447Combined sources6
Beta strandi3450 – 3462Combined sources13
Beta strandi3465 – 3469Combined sources5
Helixi3476 – 3478Combined sources3
Beta strandi3481 – 3484Combined sources4
Helixi3495 – 3507Combined sources13
Helixi3521 – 3530Combined sources10
Helixi3540 – 3549Combined sources10
Helixi3553 – 3565Combined sources13
Beta strandi3575 – 3577Combined sources3
Helixi3584 – 3591Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YNAX-ray1.50A/B3292-3597[»]
2YNBX-ray1.96A/B3292-3597[»]
ProteinModelPortaliP0C6T4.
SMRiP0C6T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1152 – 1321MacroPROSITE-ProRule annotationAdd BLAST170
Domaini1593 – 1864Peptidase C16PROSITE-ProRule annotationAdd BLAST272
Domaini3292 – 3597Peptidase C30PROSITE-ProRule annotationAdd BLAST306

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2112 – 2395HD1By similarityAdd BLAST284
Regioni2800 – 3169HD2By similarityAdd BLAST370
Regioni3603 – 3828HD3By similarityAdd BLAST226

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi960 – 1049Glu-richAdd BLAST90
Compositional biasi4161 – 4166Poly-Ser6

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1714 – 1751C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4355 – 4371By similarityAdd BLAST17
Zinc fingeri4397 – 4410By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR032505. Corona_NSP4_C.
IPR002589. Macro_dom.
IPR032592. NAR_dom.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF01661. Macro. 1 hit.
PF16251. NAR. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Replicase polyprotein 1a (identifier: P0C6T4-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSKASVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTDD MACKLTPWFE
60 70 80 90 100
DGETAFNQVS SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS
110 120 130 140 150
KHFLVNQLAY KDQVGAAMMR TTLNAKPLGM FFPYDSSLET GEYTFLLRKN
160 170 180 190 200
GLGGQLFRER PWDRKETPYV EILDDLEADP TGKYSQNLLK KLIGGDCIPI
210 220 230 240 250
DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS DRFIVLNKKL
260 270 280 290 300
YRVVWNVTRR NVPYPKQTAF TIVSVVQCDD KDSVPEHTFT IGSQILMVSP
310 320 330 340 350
LKATNNKNFN LKQRLLYTFY GKDAVQQPGY IYHSAYVDCN ACGRGTWCTG
360 370 380 390 400
NAIQGFACDC GANYSANDVD LQSSGLVPRN ALFLANCPCA NNGACSHSAA
410 420 430 440 450
QVYNILDGKA CVEVGGKSFT LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR
460 470 480 490 500
IGDAIFTGCT GTWDKVVETA NLFLEKAQRS LNFCQQFALT EVVLAILSGT
510 520 530 540 550
TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI NIGANGVCNA
560 570 580 590 600
TINAPFIAFT GLGESFKKVS AIPWKICSNL KSALDYYSSN IMFRVFPYDI
610 620 630 640 650
PCDVSNFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SSACQTALSS
660 670 680 690 700
FLNACVAASR ATAGFINDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ
710 720 730 740 750
QVLDIMSKAM KLLHTNVSWA GTKLSAIIYE GREALLFNSG TYFCLSTKAK
760 770 780 790 800
TLQGQMNLVL PGDYNKKTLG ILDPVPNADT IDVNANSTVV DVVHGQLEPT
810 820 830 840 850
NEHGPSMIVG NYVLVSDKLF VRTEDEEFYP LCTNGKVVST LFRLKGGMPS
860 870 880 890 900
KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE
910 920 930 940 950
DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN
960 970 980 990 1000
MTFSINPVED VEEVEEFIED DYLSDELPIA DDEEAWARAV EEVMPLDDIL
1010 1020 1030 1040 1050
VAEIELEEDP PLETALESVE AEVVETAEAQ EPSVESIDST PSTSTVVGEN
1060 1070 1080 1090 1100
DLSVKPMSRV AETDDVLELE TAVVGGPVSD VTAIVTNDIV SVEQAQQCGV
1110 1120 1130 1140 1150
SSLPIQDEAS ENQVHQVSDL QGNELLCSET KVEIVQPRQD LKPRRSRKSK
1160 1170 1180 1190 1200
VDLSKYKHTV INNSVTLVLG DAIQIASLLP KCILVNAANR HLKHGGGIAG
1210 1220 1230 1240 1250
VINKASGGDV QEESDEYISN NGPLHVGDSV LLKGHGLADA ILHVVGPDAR
1260 1270 1280 1290 1300
NNEDAALLKR CYKAFNKHTI VVTPLISAGI FSVDPKVSFE YLLANVTTTT
1310 1320 1330 1340 1350
YVVVNNEDIY NTLATPSKPD GLVYSFEGWR GTVRTAKNYG FTCFICTEYS
1360 1370 1380 1390 1400
ANVKFLRTKG VDTTKKIQTV DGVSYYLYSA RDALTDVIAA ANGCSGICAM
1410 1420 1430 1440 1450
PFGYVTHGLD LAQSGNYVRQ VKVPYVCLLA SKEQIPIMNS DVAIQTPETA
1460 1470 1480 1490 1500
FINNVTSNGG YHSWHLVSGD LIVKDVCYKK LLHWSGQTIC YADNKFYVVK
1510 1520 1530 1540 1550
NDVALPFSDL EACRAYLTSR AAQQVNIEVL VTIDGVNFRT VILNDTTTFR
1560 1570 1580 1590 1600
KQLGATFYKG VDISDAFPTV KMGGESLFVA DNLSESEKVV LKEYYGTSDV
1610 1620 1630 1640 1650
TFLQRYYSLQ PLVQQWKFVV HDGVKSLKLS NYNCYINATI MMIDMLHDIK
1660 1670 1680 1690 1700
FVVPALQNAY LRYKGGDPYD FLALIMAYGD CTFDNPDDEA KLLHTLLAKA
1710 1720 1730 1740 1750
ELTVSAKMVW REWCTVCGIR DIEYTGMRAC VYAGVNSMEE LQSVFNETCV
1760 1770 1780 1790 1800
CGSVKHRQLV EHSAPWLLVS GLNEVKVSTS TDPIYRAFNV FQGVETSVGH
1810 1820 1830 1840 1850
YVHIRVKDGL FYKYDSGSLT KTSDMKCKMT SVWYPTVRYT ADCNVVVYDL
1860 1870 1880 1890 1900
DGVTKVEVNP DLSNYYMKDG KYYTSKPTIK YSPATILPGS VYSNSCLVGV
1910 1920 1930 1940 1950
DGTPGSDTIS KFFNDLLGFD ETKPISKKLT YSLLPNEDGD VLLSEFSNYN
1960 1970 1980 1990 2000
PVYKKGVMLK GKPILWVNNG VCDSALNKPN RASLRQLYDV APIVLDNKYT
2010 2020 2030 2040 2050
VLQDNTSQLV EHNVPVVDDV PITTRKLIEV KCKGLNKPFV KGNFSFVNDP
2060 2070 2080 2090 2100
NGVTVVDTLG LTELRALYVD INTRYIVLRD NNWSSLFKLH TVESGDLQIV
2110 2120 2130 2140 2150
AAGGSVTRRA RVLLGASSLF ASFAKITVTA TTAACKTAGR GFCKFVVNYG
2160 2170 2180 2190 2200
VLQNMFVFLK MLFFLPFNYL WPKKQPTVDI GVSGLRTAGI VTTNIVKQCG
2210 2220 2230 2240 2250
TAAYYMLLGK FKRVDWKATL RLFLLLCTTI LLLSSIYHLV LFNQVLSSDV
2260 2270 2280 2290 2300
MLEDATGILA IYKEVRSYLG IRTLCDGLVV EYRNTSFDVM EFCSNRSVLC
2310 2320 2330 2340 2350
QWCLIGQDSL TRYSALQMLQ THITSYVLNI DWIWFALEFF LAYVLYTSSF
2360 2370 2380 2390 2400
NVLLLVVTAQ YFFAYTSAFV NWRAYNYIVS GLFFLVTHIP LHGLVRVYNF
2410 2420 2430 2440 2450
LACLWFLRKF YSHVINGCKD TACLLCYKRN RLTRVEASTI VCGTKRTFYI
2460 2470 2480 2490 2500
AANGGTSYCC KHNWNCVECD TAGVGNTFIC TEVANDLTTT LRRLIKPTDQ
2510 2520 2530 2540 2550
SHYYVDSVVV KDAVVELHYN RDGSSCYERY PLCYFTNLEK LKFKEVCKTP
2560 2570 2580 2590 2600
TGIPEHNFLI YDTNDRGQEN LARSACVYYS QVLCKPMLLV DVNLVTTVGD
2610 2620 2630 2640 2650
SREIAIKMLD SFINSFISLF SVSRDKLEKL INTARDCVRR GDDFQNVLKT
2660 2670 2680 2690 2700
FTDAARGHAG VESDVETTMV VDALQYAHKN DIQLTTECYN NYVPGYIKPD
2710 2720 2730 2740 2750
SINTLDLGCL IDLKAASVNQ TSMRNANGAC VWNSGDYMKL SDSFKRQIRI
2760 2770 2780 2790 2800
ACRKCNIPFR LTTSKLRAAD NILSVKFSAT KIVGGAPSWL LRVRDLTVKG
2810 2820 2830 2840 2850
YCILTLFVFT VAVLSWFCLP SYSIATVNFN DDRILTYKVI ENGIVRDIAP
2860 2870 2880 2890 2900
NDVCFANKYG HFSKWFNENH GGVYRNSMDC PITIAVIAGV AGARVANVPA
2910 2920 2930 2940 2950
NLAWVGKQIV LFVSRVFANT NVCFTPINEI PYDTFSDSGC VLSSECTLFR
2960 2970 2980 2990 3000
DAEGNLNPFC YDPTVLPGAS SYADMKPHVR YDMYDSDMYI KFPEVIVEST
3010 3020 3030 3040 3050
LRITKTLATQ YCRFGSCEES AAGVCISTNG SWALYNQNYS TRPGIYCGDD
3060 3070 3080 3090 3100
YFDIVRRLAI SLFQPVTYFQ LSTSLAMGLV LCVFLTAAFY YINKVKRALA
3110 3120 3130 3140 3150
DYTQCAVVAV VAALLNSLCL CFIVANPLLV APYTAMYYYA TFYLTGEPAF
3160 3170 3180 3190 3200
IMHISWYVMF GAVVPIWMLA SYTVGVMLRH LFWVLAYFSK KHVDVFTDGK
3210 3220 3230 3240 3250
LNCSFQDAAS NIFVIGKDTY VALRNAITQD SFVRYLSLFN KYKYYSGAMD
3260 3270 3280 3290 3300
TASYREACAA HLCKALQTYS ETGSDILYQP PNCSVTSSVL QSGLVKMSAP
3310 3320 3330 3340 3350
SGAVENCIVQ VTCGSMTLNG LWLDNTVWCP RHIMCPADQL TDPNYDALLI
3360 3370 3380 3390 3400
SKTNHSFIVQ KHIGAQANLR VVAHSMVGVL LKLTVDVANP STPAYTFSTV
3410 3420 3430 3440 3450
KPGASFSVLA CYNGKPTGVF TVNLRHNSTI KGSFLCGSCG SVGYTENGGV
3460 3470 3480 3490 3500
INFVYMHQME LSNGTHTGSS FDGVMYGAFE DKQTHQLQLT DKYCTINVVA
3510 3520 3530 3540 3550
WLYAAVLNGC KWFVKPTRVG IVTYNEWALS NQFTEFVGTQ SIDMLAHRTG
3560 3570 3580 3590 3600
VSVEQMLAAI QSLHAGFQGK TILGQSTLED EFTPDDVNMQ VMGVVMQSGV
3610 3620 3630 3640 3650
KRISYGFIHW LISTFVLAYV SVMQLTKFTM WTYLFETIPT QMTPLLLGFM
3660 3670 3680 3690 3700
ACVMFTVKHK HTFMSLFLLP VALCLTYANI VYEPQTLISS TLIAVANWLT
3710 3720 3730 3740 3750
PTSVYMRTTH FDFGLYISLS FVLAIIVRRL YRPSMSNLAL ALCSGVMWFY
3760 3770 3780 3790 3800
TYVIGDHSSP ITYLMFITTL TSDYTITVFA TVNLAKFISG LVFFYAPHLG
3810 3820 3830 3840 3850
FILPEVKLVL LIYLGLGYMC TMYFGVFSLL NLKLRVPLGV YDYSVSTQEF
3860 3870 3880 3890 3900
RFLTGNGLHA PRNSWEALIL NFKLLGIGGT PCIKVATVQS KLTDLKCTSV
3910 3920 3930 3940 3950
VLLTVLQQLH LESNSKAWSY CVKLHNEILA AVDPTEAFER FVCLFATLMS
3960 3970 3980 3990 4000
FSANVDLDAL ANDLFENSSV LQATLTEFSH LATYAELETA QSSYQKALNS
4010 4020 4030 4040 4050
GDASPQVLKA LQKAVNVAKN AYEKDKAVAR KLERMAEQAM TSMYKQARAE
4060 4070 4080 4090 4100
DKKAKIVSAM QTMLFGMIKK LDNDVLNGVI ANARNGCVPL SIVPLCASNK
4110 4120 4130 4140 4150
LRVVIPDISV WNKVVNWPSV SYAGSLWDIT VINNVDNEVV KPTDVVETNE
4160 4170 4180 4190 4200
SLTWPLVIEC SRSSSSAVKL QNNEIHPKGL KTMVITAGVD QVNCNSSAVA
4210 4220 4230 4240 4250
YYEPVQGHRM VMGLLSENAH LKWAKVEGKD GFINIELQPP CKFLIAGPKG
4260 4270 4280 4290 4300
PEIRYLYFVK NLNNLHRGQL LGHIAATVRL QAGANTEFAS NSTVLTLVAF
4310 4320 4330 4340 4350
AVDPAKAYLD YVGSGGTPLS NYVKMLAPKT GTGVAISVKP EATADQETYG
4360 4370 4380 4390 4400
GASVCLYCRA HIEHPDVSGV CKYKTRFVQI PAHVRDPVGF LLKNVPCNVC
4410 4420 4430
QYWVGYGCNC DALRNNTVPQ SKDTNFLNES GVLV
Note: Produced by conventional translation.
Length:4,434
Mass (Da):491,823
Last modified:June 10, 2008 - v1
Checksum:i942E92FE623EFBCD
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6W3-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6W3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Length:7,119
Mass (Da):795,200
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065505 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF065505 Genomic RNA. No translation available.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YNAX-ray1.50A/B3292-3597[»]
2YNBX-ray1.96A/B3292-3597[»]
ProteinModelPortaliP0C6T4.
SMRiP0C6T4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR032505. Corona_NSP4_C.
IPR002589. Macro_dom.
IPR032592. NAR_dom.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR009003. Peptidase_S1_PA.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF16348. Corona_NSP4_C. 1 hit.
PF01661. Macro. 1 hit.
PF16251. NAR. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTiSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiR1A_BCHK4
AccessioniPrimary (citable) accession number: P0C6T4
Secondary accession number(s): A3EX93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.