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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4

Gene

OST4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for efficient N-glycosylation. Specifically involved in maintaining stability of STT3A-containing OST complexes.Curated1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Biological processi

  • protein N-linked glycosylation via asparagine Source: UniProtKB

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4
Gene namesi
Name:OST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:32483. OST4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4LumenalSequence analysis4
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
Topological domaini26 – 37CytoplasmicSequence analysisAdd BLAST12

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • oligosaccharyltransferase complex Source: UniProtKB

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23V → K: Decreases interaction with STT3A, STT3B and RPN1. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000228474.
PharmGKBiPA165697090.

Polymorphism and mutation databases

BioMutaiOST4.
DMDMi182894143.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003286381 – 37Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4Add BLAST37

Proteomic databases

PaxDbiP0C6T2.
PeptideAtlasiP0C6T2.
PRIDEiP0C6T2.
TopDownProteomicsiP0C6T2.

Expressioni

Gene expression databases

BgeeiENSG00000228474.
GenevisibleiP0C6T2. HS.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.Curated1 Publication

Protein-protein interaction databases

BioGridi933663. 3 interactors.
STRINGi9606.ENSP00000454411.

Structurei

Secondary structure

137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 31Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LATNMR-A1-37[»]
ProteinModelPortaliP0C6T2.
SMRiP0C6T2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST4 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J6QP. Eukaryota.
ENOG410Y0TR. LUCA.
GeneTreeiENSGT00390000016516.
HOVERGENiHBG073488.
InParanoidiP0C6T2.
OrthoDBiEOG091G1C6R.
PhylomeDBiP0C6T2.

Family and domain databases

InterProiView protein in InterPro
IPR018943. Oligosaccaryltransferase.
PfamiView protein in Pfam
PF10215. Ost4. 1 hit.
SUPFAMiSSF103464. SSF103464. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C6T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
MITDVQLAIF ANMLGVSLFL LVVLYHYVAV NNPKKQE
Length:37
Mass (Da):4,193
Last modified:April 8, 2008 - v1
Checksum:iAADA830EA2A3952C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013403 Genomic DNA. No translation available.
BC015653 mRNA. No translation available.
CCDSiCCDS58703.1.
RefSeqiNP_001128165.1. NM_001134693.1.
UniGeneiHs.502948.

Genome annotation databases

EnsembliENST00000429985; ENSP00000455716; ENSG00000228474.
ENST00000447619; ENSP00000454411; ENSG00000228474.
ENST00000456793; ENSP00000457935; ENSG00000228474.
GeneIDi100128731.
KEGGihsa:100128731.
UCSCiuc002rig.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013403 Genomic DNA. No translation available.
BC015653 mRNA. No translation available.
CCDSiCCDS58703.1.
RefSeqiNP_001128165.1. NM_001134693.1.
UniGeneiHs.502948.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LATNMR-A1-37[»]
ProteinModelPortaliP0C6T2.
SMRiP0C6T2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi933663. 3 interactors.
STRINGi9606.ENSP00000454411.

Polymorphism and mutation databases

BioMutaiOST4.
DMDMi182894143.

Proteomic databases

PaxDbiP0C6T2.
PeptideAtlasiP0C6T2.
PRIDEiP0C6T2.
TopDownProteomicsiP0C6T2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000429985; ENSP00000455716; ENSG00000228474.
ENST00000447619; ENSP00000454411; ENSG00000228474.
ENST00000456793; ENSP00000457935; ENSG00000228474.
GeneIDi100128731.
KEGGihsa:100128731.
UCSCiuc002rig.4. human.

Organism-specific databases

CTDi100128731.
GeneCardsiOST4.
HGNCiHGNC:32483. OST4.
neXtProtiNX_P0C6T2.
OpenTargetsiENSG00000228474.
PharmGKBiPA165697090.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J6QP. Eukaryota.
ENOG410Y0TR. LUCA.
GeneTreeiENSGT00390000016516.
HOVERGENiHBG073488.
InParanoidiP0C6T2.
OrthoDBiEOG091G1C6R.
PhylomeDBiP0C6T2.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

ChiTaRSiOST4. human.
GenomeRNAii100128731.
PROiPR:P0C6T2.

Gene expression databases

BgeeiENSG00000228474.
GenevisibleiP0C6T2. HS.

Family and domain databases

InterProiView protein in InterPro
IPR018943. Oligosaccaryltransferase.
PfamiView protein in Pfam
PF10215. Ost4. 1 hit.
SUPFAMiSSF103464. SSF103464. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOST4_HUMAN
AccessioniPrimary (citable) accession number: P0C6T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: May 10, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.