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Protein

DNA adenine methylase

Gene

dam

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciVCHO:VC2626-MONOMER.

Protein family/group databases

REBASEi4762. M.VchADamP.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.VchADam
Gene namesi
Name:dam
Ordered Locus Names:VC_2626
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000584: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277DNA adenine methylasePRO_0000087999Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243277.VC2626.

Structurei

3D structure databases

ProteinModelPortaliP0C6Q8.
SMRiP0C6Q8. Positions 3-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C6Q8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKQRAFLKW AGGKYSLVED IQRHLPEARE LVEPFVGAGS VFLNTDFERY
60 70 80 90 100
LLADINPDLI NFYNLLKTEP QAYIHEAKRW FVPENNRKEV YLDIRKQFNQ
110 120 130 140 150
SDDAMFRSLA FLYMNRFGFN GLCRYNKKGG FNVPFGSYKK PYFPEQELEF
160 170 180 190 200
FAEKAQRATF ICASYGETFA RAQSDSVIYC DPPYAPLSTT ANFTSYAGNG
210 220 230 240 250
FTLDDQAALA DIAEKTAKER GISVLISNHD TTHTRRLYRG AQLNVVKANR
260 270
TISRNGAGRN KVDELLALFT PHLSSQA
Length:277
Mass (Da):31,624
Last modified:March 18, 2008 - v1
Checksum:i48D831240F9E7C60
GO

Sequence cautioni

The sequence CAA48031.1 differs from that shown. Reason: Frameshift at several positions. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67820 Genomic DNA. Translation: CAA48031.1. Frameshift.
AE003852 Genomic DNA. Translation: AAF95767.1.
PIRiH82052.
S29558.
RefSeqiNP_232254.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95767; AAF95767; VC_2626.
GeneIDi2615643.
KEGGivch:VC2626.
PATRICi20084276. VBIVibCho83274_2504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67820 Genomic DNA. Translation: CAA48031.1. Frameshift.
AE003852 Genomic DNA. Translation: AAF95767.1.
PIRiH82052.
S29558.
RefSeqiNP_232254.1. NC_002505.1.

3D structure databases

ProteinModelPortaliP0C6Q8.
SMRiP0C6Q8. Positions 3-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243277.VC2626.

Protein family/group databases

REBASEi4762. M.VchADamP.

Protocols and materials databases

DNASUi2615643.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95767; AAF95767; VC_2626.
GeneIDi2615643.
KEGGivch:VC2626.
PATRICi20084276. VBIVibCho83274_2504.

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.

Enzyme and pathway databases

BioCyciVCHO:VC2626-MONOMER.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA adenine methyltransferase-encoding gene (dam) of Vibrio cholerae."
    Bandyopadhyay R., Das J.
    Gene 140:67-71(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiDMA_VIBCH
AccessioniPrimary (citable) accession number: P0C6Q8
Secondary accession number(s): Q08318, Q9KNV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.