P0C6Q3 (PGK_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	VC_0477

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity. HAMAP MF_00145
Subcellular location	Cytoplasm HAMAP MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.
Sequence caution	The sequence AAF93650.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Phosphoglycerate kinase HAMAP MF_00145		PRO_0000146034
Regions
	Nucleotide binding	340 – 343	4	ATP By similarity
	Region	21 – 23	3	Substrate binding By similarity
	Region	59 – 62	4	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	197	1	ATP By similarity
	Binding site	314	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C6Q3 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 0D7EBFBF5C93F719	FASTA	387	40,979
	10 20 30 40 50 60 MSVIKMIDLD LAGKRVFIRA DLNVPVKDGK VTSDARILAS LPTIKHCLEA GAKVMVTSHL 70 80 90 100 110 120 GRPTEGEYAE EFSLLPVVNY LNDALDCEVR LVKDYLDGVE LNAGELVVLE NVRFNKGEKK 130 140 150 160 170 180 NEEALSKKYA ALCDVFVMDA FGTAHRAQAS THGVGMFAPI ACAGPLLADE LEALGKAMDK 190 200 210 220 230 240 PARPMVAIVG GSKVSTKLTV LESLSKIADQ LVVGGGIANT FIAAAGHNVG KSLYEADLVE 250 260 270 280 290 300 TAKKLMEECA IPVATDVACA KAFDENAEAE IKHVSEVQDD DMIFDLGPNS TAELAEILKN 310 320 330 340 350 360 AKTILWNGPV GVFEFKNFEA GTRGIAEAIA QSEGFSVAGG GDTLAAIDKF GIKADVSYIS 370 380 TGGGAFLEFV EGKKLPAVEM LEARAKA

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003852 Genomic DNA. Translation: AAF93650.1. Different initiation.
PIR	F82317.
RefSeq	NP_230131.1. NC_002505.1.
3D structure databases
ProteinModelPortal	P0C6Q3.
SMR	P0C6Q3. Positions 2-386.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615271.
GenomeReviews	Gene locus VC_0477 in contig AE003852_GR.
KEGG	vch:VC0477.
PATRIC	20080051. VBIVibCho83274_0451.
TIGR	VC_0477.
Phylogenomic databases
HOGENOM	HBG453500.
OMA	DCIGDEV.
PhylomeDB	P0C6Q3.
ProtClustDB	PRK00073.
Family and domain databases
HAMAP	MF_00145. Phosphoglyc_kinase. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
KO	K00927.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_VIBCH

Accession

Primary (citable) accession number: P0C6Q3
Secondary accession number(s): P96154, Q9KUN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program