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P0C6M9 (LHDAG_HDVWO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large delta antigen

Short name=L-HDAg
Alternative name(s):
p27
OrganismHepatitis delta virus genotype I (isolate Woodchuck) (HDV) [Complete proteome]
Taxonomic identifier31764 [NCBI]
Taxonomic lineageVirusesDeltavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication By similarity.

Subunit structure

Homodimer. Homooctamer. Interacts with HBV HBsAg. May interact with clathrin to induce virion budding By similarity.

Subcellular location

Virion. Host nucleushost nucleolus. Note: isoprenylated in the cytoplasm, and translocates in the nucleus possibly after phosphorylation. Translocates after to nuclear speckle, then to the ER membrane where interaction with Hepatitis B virus antigene takes place By similarity.

Post-translational modification

Prenylated by host farnesyl-transferase in the cytoplasm prior to nucleus translocation By similarity.

Phosphorylated at serines by host CK2 and other kinases. phosphorylation does not seem to be important for its function By similarity.

Miscellaneous

This strain belongs to the genotype I found in North America, Europe, Africa, East and West Asia and the South Pacific.

Sequence similarities

Belongs to the hepatitis delta antigen family.

RNA editing

Edited at position 196.
Partially edited. RNA editing at this position occurs on the antigenomic strand and consists of a conversion of A to G catalyzed by the cellular enzyme ADAR1. The unedited RNA version gives rise to the small delta antigen (AC P29997), which ends with a nonsense codon at position 196. In the edited version, this amber codon is modified to a tryptophan codon and gives rise to the large delta antigen protein. S-HDAg suppresses editing of non-replicating antigenomic RNA, thereby regulating the extent of editing By similarity. Ref.2

Ontologies

Keywords
   Biological processViral penetration into host nucleus
Virus entry into host cell
   Cellular componentHost nucleus
Virion
   Coding sequence diversityRNA editing
   LigandRNA-binding
   PTMAcetylation
Lipoprotein
Methylation
Phosphoprotein
Prenylation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processviral entry into host cell

Inferred from electronic annotation. Source: UniProtKB-KW

viral penetration into host nucleus

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

intracellular

Inferred from electronic annotation. Source: GOC

virion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Large delta antigen
PRO_0000038158
Propeptide212 – 2143Removed in mature form By similarity
PRO_0000396678

Regions

Region12 – 6049Dimerization Potential
Region97 – 10711RNA-binding Potential
Region136 – 14611RNA-binding Potential
Motif66 – 7510Nuclear localization signal By similarity

Amino acid modifications

Modified residue21Phosphoserine; by host By similarity
Modified residue131Omega-N-methylated arginine; by host By similarity
Modified residue721N6-acetyllysine; by host By similarity
Modified residue1231Phosphoserine; by host By similarity
Modified residue1771Phosphoserine; by host By similarity
Modified residue2111Cysteine methyl ester; by host By similarity
Lipidation2111S-farnesyl cysteine; by host By similarity

Natural variations

Natural variant111G → R in strain: Isolate Woodchuck5.
Natural variant861R → G in strain: Isolate Woodchuck5.
Natural variant961D → E in strain: Isolate Woodchuck5.

Sequences

Sequence LengthMass (Da)Tools
P0C6M9 [UniParc].

Last modified April 14, 2009. Version 1.
Checksum: 44360B6EF6870B5C

FASTA21424,078
        10         20         30         40         50         60 
MSRSESRKNR GGREEILEQW VAGRKKLEEL ERDLRKTKKK LKKIEDENPW LGNIKGILGK 

        70         80         90        100        110        120 
KDKDGEGAPP AKRARTDQME VDSGPRKRPL RGGFTDKERQ DHRRRKALEN KKKQLSAGGK 

       130        140        150        160        170        180 
NLSKEEEEEL RRLTEEDERR ERRVAGPPVG GVNPLEGGSR GAPGGGFVPN LQGVPESPFS 

       190        200        210 
RTGEGLDIRG NQGFPWDILF PADPPFSPQS CRPQ 

« Hide

References

[1]"Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications."
Kuo M.Y.P., Goldberg J., Coates L., Mason W., Gerin J., Taylor J.
J. Virol. 62:1855-1861(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nucleotide sequence analysis of three different hepatitis delta viruses isolated from a woodchuck and humans."
Deny P., Zignego A.L., Rascalou N., Ponzetto A., Tiollais P., Brechot C.
J. Gen. Virol. 72:735-739(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], RNA EDITING.
Strain: Isolate Woodchuck5.
[3]"Hepatitis D."
Husa P., Linhartova A., Nemecek V., Husova L.
Acta Virol. 49:219-225(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[4]"Post-translational modification of delta antigen of hepatitis D virus."
Huang W.H., Chen C.W., Wu H.L., Chen P.J.
Curr. Top. Microbiol. Immunol. 307:91-112(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M21012 Genomic RNA. Translation: AAA45723.1. Different termination.
AJ307077 Genomic RNA. Translation: CAC32838.1.
PIRSAVLWC. A30054.

3D structure databases

ProteinModelPortalP0C6M9.
SMRP0C6M9. Positions 12-60.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D4.10.220.40. 1 hit.
InterProIPR027403. Delta_antigen_N.
IPR002506. HDV_ag.
[Graphical view]
PfamPF01517. HDV_ag. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLHDAG_HDVWO
AccessionPrimary (citable) accession number: P0C6M9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: February 19, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families