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P0C6F7

- R1A_BC133

UniProt

P0C6F7 - R1A_BC133

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Protein

Replicase polyprotein 1a

Gene
1a
Organism
Bat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.1 Publication
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function By similarity.1 Publication
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.1 Publication
Nsp9 is a ssRNA-binding protein By similarity.1 Publication
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.1 Publication

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei195 – 1962Cleavage Reviewed prediction
Sitei847 – 8482Cleavage; by PL-PRO Reviewed prediction
Active sitei1641 – 16411For PL-PRO activity By similarity
Active sitei1807 – 18071For PL-PRO activity By similarity
Sitei2791 – 27922Cleavage; by PL-PRO Reviewed prediction
Sitei3298 – 32992Cleavage; by 3CL-PRO Reviewed prediction
Active sitei3339 – 33391For 3CL-PRO activity By similarity
Active sitei3446 – 34461For 3CL-PRO activity By similarity
Sitei3604 – 36052Cleavage; by 3CL-PRO Reviewed prediction
Sitei3896 – 38972Cleavage; by 3CL-PRO Reviewed prediction
Sitei3979 – 39802Cleavage; by 3CL-PRO Reviewed prediction
Sitei4178 – 41792Cleavage; by 3CL-PRO Reviewed prediction
Sitei4288 – 42892Cleavage; by 3CL-PRO Reviewed prediction
Sitei4427 – 44282Cleavage; by 3CL-PRO Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1721 – 175838C4-type By similarityAdd
BLAST
Zinc fingeri4362 – 437817 By similarityAdd
BLAST
Zinc fingeri4404 – 441714 By similarityAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA-directed RNA polymerase activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host translation Source: UniProtKB-KW
  7. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  8. viral genome replication Source: InterPro
  9. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiBat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)
Taxonomic identifieri389230 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirusunclassified Betacoronavirus
Virus hostiTylonycteris pachypus (Lesser bamboo bat) [TaxID: 258959]
ProteomesiUP000007449: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2152 – 217221Helical; Reviewed predictionAdd
BLAST
Transmembranei2229 – 224921Helical; Reviewed predictionAdd
BLAST
Transmembranei2333 – 235321Helical; Reviewed predictionAdd
BLAST
Transmembranei2357 – 237721Helical; Reviewed predictionAdd
BLAST
Transmembranei2382 – 240221Helical; Reviewed predictionAdd
BLAST
Transmembranei2807 – 282721Helical; Reviewed predictionAdd
BLAST
Transmembranei3079 – 309921Helical; Reviewed predictionAdd
BLAST
Transmembranei3112 – 313221Helical; Reviewed predictionAdd
BLAST
Transmembranei3156 – 317621Helical; Reviewed predictionAdd
BLAST
Transmembranei3610 – 363021Helical; Reviewed predictionAdd
BLAST
Transmembranei3644 – 366421Helical; Reviewed predictionAdd
BLAST
Transmembranei3669 – 368921Helical; Reviewed predictionAdd
BLAST
Transmembranei3714 – 373421Helical; Reviewed predictionAdd
BLAST
Transmembranei3742 – 376221Helical; Reviewed predictionAdd
BLAST
Transmembranei3791 – 381121Helical; Reviewed predictionAdd
BLAST
Transmembranei3815 – 383521Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell membrane Source: UniProtKB-SubCell
  2. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 44414441Replicase polyprotein 1aPRO_0000338062Add
BLAST
Chaini1 – 195195Non-structural protein 1 Reviewed predictionPRO_0000338063Add
BLAST
Chaini196 – 847652Non-structural protein 2 Reviewed predictionPRO_0000338064Add
BLAST
Chaini848 – 27911944Non-structural protein 3 Reviewed predictionPRO_0000338065Add
BLAST
Chaini2792 – 3298507Non-structural protein 4 Reviewed predictionPRO_0000338066Add
BLAST
Chaini3299 – 36043063C-like proteinase Reviewed predictionPRO_0000338067Add
BLAST
Chaini3605 – 3896292Non-structural protein 6 Reviewed predictionPRO_0000338068Add
BLAST
Chaini3897 – 397983Non-structural protein 7 Reviewed predictionPRO_0000338069Add
BLAST
Chaini3980 – 4178199Non-structural protein 8 Reviewed predictionPRO_0000338070Add
BLAST
Chaini4179 – 4288110Non-structural protein 9 Reviewed predictionPRO_0000338071Add
BLAST
Chaini4288 – 4441154Non-structural protein 11 Reviewed predictionPRO_0000338073Add
BLAST
Chaini4289 – 4427139Non-structural protein 10 Reviewed predictionPRO_0000338072Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP0C6F7.
SMRiP0C6F7. Positions 3896-3979, 4228-4288, 4298-4418.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1159 – 1328170MacroAdd
BLAST
Domaini1600 – 1871272Peptidase C16Add
BLAST
Domaini3299 – 3604306Peptidase C30Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2119 – 2402284HD1 By similarityAdd
BLAST
Regioni2807 – 3176370HD2 By similarityAdd
BLAST
Regioni3610 – 3835226HD3 By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi960 – 1059100Glu-richAdd
BLAST

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane By similarity.

Sequence similaritiesi

Contains 1 Macro domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1721 – 175838C4-type By similarityAdd
BLAST
Zinc fingeri4362 – 437817 By similarityAdd
BLAST
Zinc fingeri4404 – 441714 By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR002589. Macro_dom.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1a (identifier: P0C6F7-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MLSKAGVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTED MASKLTPWFE     50
DGETAFNQVS SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS 100
KHFLVNQLAY KDHVGAAMMR TTLNVKPLGM FFPYDSSLET GEHTFLLRKN 150
GLGGQLFRER PWDRKETPYV EILDDLEADP TGKYSQNLLK KLIGGDCIPV 200
DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS DRFIVLNKKL 250
YRVVWNVTRR NVPYSKQTAF TVVSVIQCDD KESVPEHTFT IGSQILMVSP 300
LKATNNKNFN LKQRLLHTFY GKEAVQQPGY IYHSAYVDCN ACGRGTWCTG 350
NAIQGFACDC GANYSANDVD LQSSGLVPKN ALFLANCPCA NNGACSHNAA 400
QVYSILDGKA CVEVGGKSFT LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR 450
IGDAIFTGCT GTWDKVVETA NLFLEKAQHS LNFCQQFALT EVVLAILSGT 500
TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI NIGANGVCNA 550
TINAPFIAFT GLGESFKKVA AIPWKICSNL KSALDYYCSN IMFRVFPYDI 600
PCDVNDFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SNACQTALSS 650
FLNACVAASR ATAGFISDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ 700
QVLDIMSKAM KLLHTNVSWA GTKLSAIIYE GREALLFNSG TYFCLSTKAK 750
TLQDQMNLVL PGDYNKKTLG ILDPVPNADT IDVTANSTVV DVVHGQLEPT 800
NEHGPSMIVG NYVLVSDKLF VRTDDEEFYP LCINGKVVST LFRLKGGMPS 850
KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE 900
DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN 950
MTFSINPVED VEEVEEFIED DYLSDELPIA DDEEAWTRAV EEVMPLDDIL 1000
VAEIELEEDL PLETALESVE AEVGESISDE LCVVETAKAQ EPSVESTDST 1050
PSTSTVVSEN DLSVKPMSRV AETGDVLEVE TAVVGGPVSD VTASVVTNDI 1100
VSVEQAQQCG VSSLPIQDEA SENQVHQVPD LQCTSETKVE IVQPRQDLRP 1150
RRLRKSKVDL SKYKHTVINN SVTLVLGDAI QIASLLPKCV LVNAANRHLK 1200
HGGGIAGAIN KASGGDVQEE SDEYISNSGP LHVGDSVLLK GYGLADAILR 1250
VVGPDARNNE DAALLKRCYK TFNKHTIVVT PLISSGIFSV DPKVSFEYLL 1300
ANVTTTTYVV VNNEDIYNTL ATPSKPDGLV YSFEGWRGTV RTAKNYGFTC 1350
FICTEYSANV KFLRTKGVDT TKKIQTVDGV SYYLYSARDA LTDVIAAANG 1400
CPGICAMPFG YVTHGLDLAQ SGNYVRQVKV PYVCLLASKE QIPIMNSDVA 1450
IQTPETAFIN NVTSNGGYHS WHLVSGDLIV KDVCYKKLLH WSGQTICYAD 1500
NKFYVVKNDV ALPFSDLEAC RAYLTSRAAQ QVNIEVLVTI DGVNFRTVIL 1550
NDATTFRKQL GATFYKGVDI SDALPTVKMG GESLFVADNL SESEEVVLKE 1600
YYGTSDVTFL QRYYSLQPLV QQWKFVVHDG VKSLKLSNYN CYINATIMMI 1650
DMLHDIKFVV PALQNAYLRY KGGDPYDFLA LIMAYGDCTF DNPDDEAKLL 1700
HTLLAKAELT VSAKMVWREW CTVCGIRDIE YTGMRACVYA GVNSMEELQS 1750
VFNETCVCGS VKHRQLVEHS TPWLLVSGLN EVKVSTSTDP VYRAFNVFQG 1800
VETSVGHYVH VRVKDGLFYK YDSGSLTKTS DMKCKMTSVW YPKVRYTADC 1850
NVVVYDLDGV TKVEVNPDLS NYYMKDGKYY TSKPTIKYSP ATILPGSVYS 1900
NSCLVGVDGT PGSDTISKFF NDLLGFDETK PISKKLTYSL LPNEDGDVLL 1950
SEFNNYNPVY KKGVMLKGKP ILWVNNGVCD SALNKPNRAS LRQLYDVAPI 2000
VLDNKYTVLQ DNTSQLIEPN VPVVEDVSIT TRKLIEVKCK GLNKPFVKGN 2050
FSFVNDPNGV TVVDTLGLTE LRALYVDINT RYIVLRDNNW SSLFKLHTVE 2100
SGDLQIVANG GSVTRRARVL LGASSLFASF AKITVTATTA ACKTAGRSFC 2150
KFVVNYGVLQ NMFLFLKMLF FLPFNYLWPK KQPTVDVGVS GLRTAGVVTT 2200
NIVKQCGTAA YYMLLGKFKR VDWKATLRLF LLLCTTILLL SSIYHLVIFN 2250
QVLSSDVMLE DATGILAMYK EVRSYLGIRT LCDGLAVEYR NTSFDVVDFC 2300
SNRSVLCQWC LIGQDSLTRY SALQMLQTHI TSYVLNIDWI WFALEFFLAY 2350
VLYTSSFNVL LLVVTAQYFF AYTSAFVNWR AYNYIVSGLF FLVTHIPLHG 2400
LVRVYNFLAC LWFLRKFYSH VINGCKDTAC LLCYKRNRLT RVEASTIVCG 2450
TKRTFYIAAN GGTSYCCKHN WNCVECDTAG VGNTFICTEV ANDLTTTLRR 2500
LIKPTDQSHY YVDSVVVKDA VVELHYNRDG SSCYERYPLC YFTNLEKLKF 2550
KEVCKTPTGI PEHNFLIYDT NDRGQENLAR SACVYYSQVL CKPMLLVDVN 2600
LVTTVGDSRE IAIKMLDSFI NSFISLFSVS RDKLEKLINT ARDCVRRGDD 2650
FQTVLKTFTD AARGHAGVES DVETTMVVDA LQYAHKNDIQ LTTECYNNYV 2700
PGYIKPDSIN TLDLGCLIDL KAASVNQTSM RNANGACVWN SGDYMKLSDS 2750
FKRQIRIACR KCNIPFRLTT SKLRAADNIL SVKFSATKIV GGAPSWLLRV 2800
RDLTVKGYCI LTLFVFTVAV LSWFCLPSYS IATVNFNDDR ILTYKVIENG 2850
IVRDIAPNDA CFANKYGHFS KWFNENHGGV YRNSVDCPIT IAVIAGVAGA 2900
RVANVPATLA WVGRQIVLFV SRVFANTNVC FTPTNEIPYD TFSDSGCVLS 2950
SECTLFRDAE GNLNPFCYDP TVLPGASSYA DMKPHVRYDM YDSDMYIKFP 3000
EVIFESTLRI TKTLATQYCR FGSCEESAAG VCISTNGSWA LYNQNYSTRP 3050
GIYCGDDYFD IVRRLAVSLF QPVTYFQLST SLAMGLVLCV FLTAAFYYIN 3100
KVKRALADYT QCAVVAVVAA LLNSLCLCFI VANPLLVAPY TAMYYYATFY 3150
LTGEPAFIMH ISWYVMFGTV VPIWMLASYT VGVMLRHLFW VLAYFSKKHV 3200
DVFTDGKLNC SFQDAASNIF VIGKDTYVAL RNAITQDSFV RYLSLFNKYK 3250
YYSGAMDTAS YREACAAHLC KALQTYSETG SDILYQPPNC SVTSSVLQSG 3300
LVKMSAPSGA VENCIVQVTC GSMTLNGLWL DNTVWCPRHI MCPADQLTDP 3350
NYDALLISKT NHSFIVQKHI GAQANLRVVA HSMVGVLLKL TVDVANPSTP 3400
AYTFSTVKPG ASFSVLACYN GKPTGVFTVN LRHNSTIKGS FLCGSCGSVG 3450
YTENGGVLNF VYMHQMELSN GTHTGSSFDG VMYGAFEDKQ THQLQLTDKY 3500
CTINVVAWLY AAVLNGCKWF VKPTRVGIVT YNEWALSNQF TEFVGTQSID 3550
MLAHRTGVSV EQMLAAIQSL HAGFQGKTIL GQSTLEDEFT PDDVNMQVMG 3600
VVMQSGVKRI SYGFMHWLMS TLVLAYVSVM QLTKFTMWTY LFETIPTQMT 3650
PLLFGFMACV MFTVKHKHTF LSLFLLPVAL CLTYANIVYE PQTLVSSTLI 3700
AVANWLTPTS VYMRTTHLDF GLYISLSFVL AIIVRRLYRP SMSNLALALC 3750
SGVMWFYTYV IGDHSSPITY LMFITTLTSD YTITVFATVN LAKFISGLVF 3800
LYAPHLGFIL PEVKLVLLIY LCLGYMCTMY FGVFSLLNLK LRVPLGVYDY 3850
SVSTQEFRFL TGNGLHAPRN SWEALILNFK LLGIGGTPCI KVATVQSKLT 3900
DLKCTSVVLL TVLQQLHLES NSKAWSYCVK LHNEILAAVD PTEAFERFVC 3950
LFATLMSFSA NVDLDALAND LFENSSVLQA TLTEFSHLAT YAELETAQSS 4000
YQKALNSGDA SPQVLKALQK AVNVAKNAYE KDKAVARKLE RMAEQAMTSM 4050
YKQARAEDKK AKIVSAMQTM LFGMIKKLDN DVLNGVIANA RNGCVPLSIV 4100
PLCASNKLRV VIPDISVWNK VVNWPSVSYA GSLWDVTVIN NVDNEVVKPT 4150
DVVETNESLT WPLVIECSRA SSSAVKLQNN EIHPKGLKTM VVTAGIDQVN 4200
CSSSAVAYYE PVQGHRMVMG LLSENAHLKW AKVEGKDGFI NIELQPPCKF 4250
LIAGPKGPEI RYLYFVKNLN NLHRGQLLGH IAATVRLQAG ANTEFASNST 4300
VLTLVAFAVD PAKAYLDYVG SGGTPLSNYV KMLAPKTGTG VAISVKPEAT 4350
ADQETYGGAS VCLYCRAHIE HPDVSGVCKY KTRFVQIPAH VRDPVGFLLK 4400
NVPCNVCQYW VGYGCNCDAL RNNTVPQSKD TNFLNESGVL V 4441

Note: Produced by conventional translation.

Length:4,441
Mass (Da):492,464
Last modified:June 10, 2008 - v1
Checksum:iA6E0DB4CDDA40AC7
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6W1-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

The sequence of this isoform can be found in the external entry P0C6W1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,126
Mass (Da):795,781
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648794 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648794 Genomic RNA. No translation available.

3D structure databases

ProteinModelPortali P0C6F7.
SMRi P0C6F7. Positions 3896-3979, 4228-4288, 4298-4418.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002589. Macro_dom.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEi PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Suppression of host gene expression by nsp1 proteins of group 2 bat coronaviruses."
    Tohya Y., Narayanan K., Kamitani W., Huang C., Lokugamage K., Makino S.
    J. Virol. 83:5282-5288(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF NSP1.

Entry informationi

Entry nameiR1A_BC133
AccessioniPrimary (citable) accession number: P0C6F7
Secondary accession number(s): Q0Q4F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi