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P0C6F5

- R1A_BC279

UniProt

P0C6F5 - R1A_BC279

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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity

Catalytic activityi

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1802CleavageBy similarity
Sitei818 – 8192Cleavage; by PL-PROBy similarity
Active sitei1657 – 16571For PL-PRO activityPROSITE-ProRule annotation
Active sitei1818 – 18181For PL-PRO activityPROSITE-ProRule annotation
Sitei3246 – 32472Cleavage; by PL-PROBy similarity
Active sitei3287 – 32871For 3CL-PRO activityPROSITE-ProRule annotation
Active sitei3391 – 33911For 3CL-PRO activityPROSITE-ProRule annotation
Sitei3552 – 35532Cleavage; by 3CL-PROBy similarity
Sitei3842 – 38432Cleavage; by 3CL-PROBy similarity
Sitei3925 – 39262Cleavage; by 3CL-PROBy similarity
Sitei4123 – 41242Cleavage; by 3CL-PROBy similarity
Sitei4236 – 42372Cleavage; by 3CL-PROBy similarity
Sitei4375 – 43762Cleavage; by 3CL-PROBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1735 – 177238C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4310 – 432617By similarityAdd
BLAST
Zinc fingeri4353 – 436614By similarityAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. hydrolase activity, acting on acid anhydrides Source: InterPro
  3. hydrolase activity, acting on ester bonds Source: InterPro
  4. omega peptidase activity Source: InterPro
  5. RNA binding Source: UniProtKB-KW
  6. RNA-directed RNA polymerase activity Source: InterPro
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. induction by virus of catabolism of host mRNA Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB-KW
  3. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
  4. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  5. suppression by virus of host ISG15 activity Source: UniProtKB-KW
  6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  7. viral genome replication Source: InterPro
  8. viral protein processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Activation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Protein family/group databases

MEROPSiC16.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
Non-structural protein 11
Short name:
nsp11
Gene namesi
ORF Names:1a
OrganismiBat coronavirus 279/2005 (BtCoV) (BtCoV/279/2005)
Taxonomic identifieri389167 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiRhinolophus macrotis (Big-eared horseshoe bat) [TaxID: 196889]
ProteomesiUP000006573: Genome

Subcellular locationi

Chain Non-structural protein 7 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 8 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 9 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Chain Non-structural protein 10 : Host cytoplasmhost perinuclear region By similarity
Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 43884388Replicase polyprotein 1aPRO_0000338038Add
BLAST
Chaini1 – 179179Non-structural protein 1By similarityPRO_0000338039Add
BLAST
Chaini180 – 818639Non-structural protein 2By similarityPRO_0000338040Add
BLAST
Chaini819 – 27461928Non-structural protein 3By similarityPRO_0000338041Add
BLAST
Chaini2747 – 3246500Non-structural protein 4By similarityPRO_0000338042Add
BLAST
Chaini3247 – 35523063C-like proteinaseBy similarityPRO_0000338043Add
BLAST
Chaini3553 – 3842290Non-structural protein 6By similarityPRO_0000338044Add
BLAST
Chaini3843 – 392583Non-structural protein 7By similarityPRO_0000338045Add
BLAST
Chaini3926 – 4123198Non-structural protein 8By similarityPRO_0000338046Add
BLAST
Chaini4124 – 4236113Non-structural protein 9By similarityPRO_0000338047Add
BLAST
Chaini4237 – 4375139Non-structural protein 10By similarityPRO_0000338048Add
BLAST
Chaini4376 – 438813Non-structural protein 11Sequence AnalysisPRO_0000338049Add
BLAST

Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Interactioni

Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C6F5.
SMRiP0C6F5. Positions 13-127, 819-930, 1000-1174, 1337-1475, 1547-1860, 3247-3552, 3842-3925, 3927-4117, 4124-4236, 4246-4368.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2209 – 222921HelicalSequence AnalysisAdd
BLAST
Transmembranei2310 – 233021HelicalSequence AnalysisAdd
BLAST
Transmembranei2357 – 237721HelicalSequence AnalysisAdd
BLAST
Transmembranei2761 – 278121HelicalSequence AnalysisAdd
BLAST
Transmembranei2998 – 301821HelicalSequence AnalysisAdd
BLAST
Transmembranei3028 – 304821HelicalSequence AnalysisAdd
BLAST
Transmembranei3060 – 308021HelicalSequence AnalysisAdd
BLAST
Transmembranei3083 – 310321HelicalSequence AnalysisAdd
BLAST
Transmembranei3111 – 313121HelicalSequence AnalysisAdd
BLAST
Transmembranei3148 – 316821HelicalSequence AnalysisAdd
BLAST
Transmembranei3570 – 359021HelicalSequence AnalysisAdd
BLAST
Transmembranei3592 – 361221HelicalSequence AnalysisAdd
BLAST
Transmembranei3618 – 363821HelicalSequence AnalysisAdd
BLAST
Transmembranei3665 – 368420HelicalSequence AnalysisAdd
BLAST
Transmembranei3691 – 371020HelicalSequence AnalysisAdd
BLAST
Transmembranei3734 – 375421HelicalSequence AnalysisAdd
BLAST
Transmembranei3762 – 378221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1001 – 1167167MacroPROSITE-ProRule annotationAdd
BLAST
Domaini1617 – 1881265Peptidase C16PROSITE-ProRule annotationAdd
BLAST
Domaini3247 – 3552306Peptidase C30PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2098 – 2377280HD1By similarityAdd
BLAST
Regioni2761 – 3168408HD2By similarityAdd
BLAST
Regioni3570 – 3782213HD3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi930 – 99970Glu-richAdd
BLAST
Compositional biasi2216 – 22194Poly-Leu
Compositional biasi3772 – 37754Poly-Cys

Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

Sequence similaritiesi

Contains 1 Macro domain.PROSITE-ProRule annotation
Contains 1 peptidase C16 domain.PROSITE-ProRule annotation
Contains 1 peptidase C30 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1735 – 177238C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri4310 – 432617By similarityAdd
BLAST
Zinc fingeri4353 – 436614By similarityAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

InterProiIPR002589. Macro_dom.
IPR021590. NSP1.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamiPF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SUPFAMiSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform Replicase polyprotein 1a (identifier: P0C6F5-1) [UniParc]FASTAAdd to Basket

Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MESLALGVSE KTHVQLSLPV LQVRDVLVRG FGDSVEEALA EAREHLKNGT
60 70 80 90 100
CGLVELEKGV LPQLEQPYVF IKRSDAQGTN HGYKVVELVA ELDGIQYGRS
110 120 130 140 150
GTTLGVLVPH VGETPVAYRN VLLRKNGNKG AGGHSYGIDL KSYDLGVELG
160 170 180 190 200
TDPIEDYEQN WNTKHGGGVL RELIRELNGG AFTRYVDNNF CGPDGYPLEC
210 220 230 240 250
IKDLLARAGK SMCTLSEQLD YIESKRGVYC CREHEHEIVW FTERSEKSYE
260 270 280 290 300
RQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI
310 320 330 340 350
RSVYPVATPQ ECNDMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV
360 370 380 390 400
CEGPTTCGYL PANAVVKMPC PACQDPEVGP EHSVADYHNH SNIETRLRKG
410 420 430 440 450
GRTKCFGGCV FAYVGCYNKR AYWVPRASAN IGASHTGITG DNVETLNEDL
460 470 480 490 500
MEILNRDRVN INIVGDFHLN EEVAIILASF SASTCAFVDT VKGLDYKTFK
510 520 530 540 550
DIVESCGNFK VTRGRAKKGA WNIGQEKSIL TPLYGFPSQA AGVIRSIFTR
560 570 580 590 600
ALDTANHSIP DLQRAAITIL DGISEQSLRL IDAMVYTSDL LTNSVIVMAY
610 620 630 640 650
VTGGLVQQIT QWLSNMLGTT VDKLKPVFTW VEAKLSAGIE FLRDAWEILK
660 670 680 690 700
FLVTGVFDIV KGQIQVASDN LKECVKAFLD VLNKALEMCI DQVIIAGAKL
710 720 730 740 750
RTLNLGEVFI AQSKGLYRQC IRGKEQLQLL MPLRAPKEVT FFEGDSHDTV
760 770 780 790 800
FTSEEVVLKN GELEALETPV DSFTNGAVIG TPVCVNGLML LELKDKEQYC
810 820 830 840 850
ALSPGLLATN NVFSLKGGAP VKGVTFGEDT VLEVQGYKNV KITFELDERV
860 870 880 890 900
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTNMGIDLDE
910 920 930 940 950
WSVATFYLFD DAGEEKLSSR MYCSFYPPDE EEDCEEYEDE EEIPEETCEH
960 970 980 990 1000
EYGTEDDYKG LPLEFGASTE IQQVDEEEEE DWLEEAIAAK PEPEPLPEEP
1010 1020 1030 1040 1050
VNQFTGYLKL TDNVAIKCVD IVKEAQHAKP TVIVNAANVH LKHGGGVAGA
1060 1070 1080 1090 1100
LNKATNGAMQ QESDDYIKKN GPLTVGGSCL LSGHNLAKKC MHVVGPNLNA
1110 1120 1130 1140 1150
GEDVQLLKAA YANFNSQDVL LAPLLSAGIF GAKPLQSLKM CVETVRTQVY
1160 1170 1180 1190 1200
FAVNDQDLYD HVVLGYLDSL KPKVETPTQE NLELKEQPAV ETLTQENLEL
1210 1220 1230 1240 1250
EELPVIEKPV DVKFKARIEE VNTSLEETKF LTSRLLLFAD INGKLYQDSQ
1260 1270 1280 1290 1300
NMLRGEDMFF LEKDAPYIVG DVISSGDITC VIIPAKKAGG TTEMLAKALK
1310 1320 1330 1340 1350
KVPVSEYITT YPGQGCAGYT LEEAKTALRK CKSVFYVLPS KTPNDKEEIL
1360 1370 1380 1390 1400
GTVSWNLREM LAHAEETRKL MLICMDVKAL MSTIHRRYKG IKVQEGIVDY
1410 1420 1430 1440 1450
GVRFFFYTSK EPVASIITKL NLLNEPLVTM PIGYVTHGLN LEEAARCMRS
1460 1470 1480 1490 1500
LKAPAVVSVS SPDAVTTYNG YLTSSSKTSE EHFIETVSLA GMYRDWSYSG
1510 1520 1530 1540 1550
QRTELGVEFL KRGDKVVYHT VGSPIQFHLD GEVLLLDKLK SLLSLREVRT
1560 1570 1580 1590 1600
IKVFTTVDNT NLHTQIVDMS MTYGQQFGPT YLDGADVTKI KPHAKHEGKT
1610 1620 1630 1640 1650
FFVLPSDDTL RSEAFEYYHT LDESFLGRYM SALNHTKKWK FPQIGGLTSI
1660 1670 1680 1690 1700
KWADNNCYLS SVLLALQQIE VKFNAPALQE AYYRARAGDA ANFCALILAY
1710 1720 1730 1740 1750
SNRTVGELGD VRETMTHLLQ HANLESAKRV LNVVCKTCGQ KSTTLTGVEA
1760 1770 1780 1790 1800
VMYMGTLSYE ELKTGVTIPC ICGRDATQYL VQQESSFVMM SAPPSEYTLQ
1810 1820 1830 1840 1850
QGAFLCANEY TGSYQCGHYT HVTVKETLYR IDGAYLTKMS EYKGPVTDVF
1860 1870 1880 1890 1900
YKEISYTTTI KPVSYKLDGV IYTEIQPKLD EYYKKDNAYY TEQPIDLVPT
1910 1920 1930 1940 1950
QPLPNASFDN FKLTCSNTKF ADDLNQMTGF KKPASRELSV TFFPDLNGDV
1960 1970 1980 1990 2000
VAIDYRHYSA SFKKGAKLLH KPIIWHINQT TNKTTYKPNT WCLRCLWSTK
2010 2020 2030 2040 2050
PVETSNSFEV LEVEDTQGMD NLACESQTPT SEEVVENPTI QKEVIECDVK
2060 2070 2080 2090 2100
TIEVVGNVIL KPSEEGVKVT QELGHEDLMA AYVEETSITI KKPNELSLAL
2110 2120 2130 2140 2150
GLRTLATHGA AAINSVPWSK ILAYVKPFLG QAAVTTTNCI KRCVQRVFNN
2160 2170 2180 2190 2200
YMPYVITLLF QLCTFTRSTN SRIRASLPTT IAKNSVKSVA KLCLDVCINY
2210 2220 2230 2240 2250
VKSPKFSKLF TIAMWLLLLS ICLGSLIYVT AAFGVLLSNL GIPSYCDGVR
2260 2270 2280 2290 2300
ESYVNSSNVT TMDFCEGSFL CSVCLNGLDS LDSYPALETI QVTISSYKLD
2310 2320 2330 2340 2350
LTSLGLAAEW FLAYMLFTKF FYLLGLSAIM QVFFGYFASH FISNSWLMWF
2360 2370 2380 2390 2400
IISIVQMAPV SAMVRMYIFF AFCYYVWKSY VHIMDGCTSS TCMMCYKRNR
2410 2420 2430 2440 2450
ATRVECTTIV NGMKRSFYVY ANGGRGFCKA HNWNCLNCDT FCAGSTFISD
2460 2470 2480 2490 2500
EVARDLSLQF KRPINPTDQS SYVVDSVAVK NGALHLYFDK AGQKTYERHP
2510 2520 2530 2540 2550
LSHFVNLDNL RANNTKGSLP INVIVFDGKS KCDESAAKSA SVYYSQLMCQ
2560 2570 2580 2590 2600
PILLLDQALV SDVGDSTEVS VKMFDAYVDT FSATFSVPME KLKALVATAH
2610 2620 2630 2640 2650
SELAKGVALD GVLSTFVSAA RQGVVDTDVD TKDVIECLKL SHHSDLEVTG
2660 2670 2680 2690 2700
DSCNNFMLTY NKVENMTPRD LGACIDCNAR HINAQVAKSH NVSLIWNVKD
2710 2720 2730 2740 2750
YMSLSEQLRK QIRSAAKKNN IPFRLTCATT RQVVNAITTK ISLKGGKIVS
2760 2770 2780 2790 2800
TWFKLMLKAT LLCVLAALFC YIIMPVHSLS VHDGYTNEII GYKAIQDGVT
2810 2820 2830 2840 2850
RDIMATDDCF ANKHAGFDSW FSQRGGSYRN DKSCPVVAAI ITREIGFIVP
2860 2870 2880 2890 2900
GLPGTVLRAI NGDFLHFLPR VFSAVGNICY TPSKLIEYSD FATSACVLAA
2910 2920 2930 2940 2950
ECTIFKDAMG KPVPYCYDTN LLEGSISYSE LRPDTRYVLM DGSIIQFPNT
2960 2970 2980 2990 3000
YLEGSVRVVT TFDAEYCRHG TCERSEAGVC LSTSGRWVLN NEHYRALPGV
3010 3020 3030 3040 3050
FCGVDAMNLI ANIFTPLVQP VGALDVSASV VAGGIIAILV TCAAYYFMKF
3060 3070 3080 3090 3100
RRAFGEYNHV VAANALLFLM SFTILCLAPA YSFLPGVYSI FYLYLTFYFT
3110 3120 3130 3140 3150
NDVSFLAHLQ WFAMFSPIVP FWITAIYVFC ISLKHCHWFF NNYLRKRVMF
3160 3170 3180 3190 3200
NGVTFSTFEE AALCTFLLNK EMYLKLRSET LLPLTQYNRY LALYNKYKYF
3210 3220 3230 3240 3250
SGALDTTSYR EAACCHLAKA LNDFSNSGAD VLYQPPQTSI TSAVLQSGFR
3260 3270 3280 3290 3300
KMAFPSGKVE GCMVQVTCGT TTLNGLWLDD TVYCPRHVIC TAEDMLNPNY
3310 3320 3330 3340 3350
EDLLIRKSNH SFLVQAGNVQ LRVIGHSMQN CLLRLKVDTS NPKTPKYKFV
3360 3370 3380 3390 3400
RIQPGQTFSV LACYNGSPSG VYQCAMRPNY TIKGSFLNGS CGSVGFNIDY
3410 3420 3430 3440 3450
DCVSFCYMHH MELPTGVHAG TDLEGKFYGP FVDRQTAQAA GTDTTITLNV
3460 3470 3480 3490 3500
LAWLYAAVIN GDRWFLNRFT TTLNDFNLVA MKYNYEPLTQ DHVDILGPLS
3510 3520 3530 3540 3550
AQTGIAVLDM CAALKELLQN GMNGRTILGS TILEDEFTPF DVVRQCSGVT
3560 3570 3580 3590 3600
FQGKFKKIVK GTHHWMLLTF LTSLLILVQS TQWSLFFFVY ENAFLPFTLG
3610 3620 3630 3640 3650
IMAIAACAML LVKHKHAFLC LFLLPSLATV AYFNMVYMPA SWVMRIMTWL
3660 3670 3680 3690 3700
ELADTSLSGY RLKDCVMYAS ALVLLVLMTA RTVYDDAARR VWTLMNVITL
3710 3720 3730 3740 3750
VYKVYYGNSL DQAISMWALV ISVTSNYSGV VTTIMFLARA IVFVCVEYYP
3760 3770 3780 3790 3800
LLFITGNTLQ CIMLVYCFLG YCCCCYFGLF CLLNRYFRLT LGVYDYLVST
3810 3820 3830 3840 3850
QEFRYMNSQG LLPPKSSIDA FKLNIKLLGI GGKPCIKVAT VQSKMSDVKC
3860 3870 3880 3890 3900
TSVVLLSVLQ QLRVESSSKL WAQCVQLHND ILLAKDTTEA FEKMVSLLSV
3910 3920 3930 3940 3950
LLSMQGAVDI NKLCEEMLDN RATLQAIASE FSSLPSYAAY ATAQEAYEQA
3960 3970 3980 3990 4000
VANGDSEVVL KKLKKSLNVA KSEFDRDAAM QRKLEKMADQ AMTQMYKQAR
4010 4020 4030 4040 4050
SEDKRAKVTS AMQTMLFTML RKLDNDALNN IINNARDGCV PLNIIPLTTA
4060 4070 4080 4090 4100
AKLMVVVPDY GTYKNTCDGN TFTYASALWE IQQVVDADSK IVQLSEINMD
4110 4120 4130 4140 4150
NSQNLAWPLI VTALRANSAV KLQNNELSPV ALRQMSCAAG TTQTACTDDN
4160 4170 4180 4190 4200
ALAYYNNSKG GRFVLALLSD HQDLKWARFP KSDGTGTIYT ELEPPCRFVT
4210 4220 4230 4240 4250
DTPRGPKVKY LYFIKGLNNL NRGMVLGSLA ATVRLQAGNA TEVPANSAVL
4260 4270 4280 4290 4300
SFCAFAVDPA KAYKDYLASG GQPITNCVKM LCTHTGTGQA ITVTPEANMD
4310 4320 4330 4340 4350
QESFGGASCC LYCRCHIDHP NPKGFCDLKG KYVQIPATCA NDPVGFTLKN
4360 4370 4380
TVCTVCGTWK GYGCSCDQLR EPMMQSADAS TFLNGFAV

Note: Produced by conventional translation.

Length:4,388
Mass (Da):487,709
Last modified:June 10, 2008 - v1
Checksum:i1D91B831273D6F2A
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6V9-1) [UniParc]FASTAAdd to Basket

Also known as: pp1ab

The sequence of this isoform can be found in the external entry P0C6V9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.

Length:7,079
Mass (Da):791,654
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648857 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ648857 Genomic RNA. No translation available.

3D structure databases

ProteinModelPortali P0C6F5.
SMRi P0C6F5. Positions 13-127, 819-930, 1000-1174, 1337-1475, 1547-1860, 3247-3552, 3842-3925, 3927-4117, 4124-4236, 4246-4368.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C16.009.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002589. Macro_dom.
IPR021590. NSP1.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR024358. SARS-CoV_Nsp3_N.
IPR022733. SARS_polyprot_cleavage.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view ]
Pfami PF12379. DUF3655. 1 hit.
PF01661. Macro. 1 hit.
PF11501. Nsp1. 1 hit.
PF09401. NSP10. 1 hit.
PF12124. Nsp3_PL2pro. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view ]
SUPFAMi SSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEi PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiR1A_BC279
AccessioniPrimary (citable) accession number: P0C6F5
Secondary accession number(s): Q0Q476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Bat coronavirus 279/2005 is highly similar to SARS-CoV (SARS-like).

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3